CN105039485A - Method for improving extraction yield of yam diosgenin - Google Patents
Method for improving extraction yield of yam diosgenin Download PDFInfo
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- CN105039485A CN105039485A CN201510375903.2A CN201510375903A CN105039485A CN 105039485 A CN105039485 A CN 105039485A CN 201510375903 A CN201510375903 A CN 201510375903A CN 105039485 A CN105039485 A CN 105039485A
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Abstract
The invention provides a method for improving the extraction yield of yam diosgenin. The method comprises the following steps: firstly, cleaning and decontaminating fresh yam to obtain crushed objects; secondly, pre-treating the crushed objects for 24 hours by using a method for carrying out single segmented temperature control and enabling the pH to be constant as 5 to 6 as well as a segmented temperature control and coordinate pH regulation method, then transferring the fermented crushed objects into a hydrolysis bottle for acid hydrolysis, regulating a separated hydrolysate to be neutral, and drying the hydrolysate to obtain a preliminary extract; finally, drying the extract to obtain the yam diosgenin. According to the method provided by the invention, the process flow is simple, and the energy consumption is low; compared with the yield of the yam diosgenin in a conventional pre-fermentation process, the yield of the yam diosgenin is improved by over 30 percent.
Description
Technical field
The invention provides a kind of method of producing turmeric saponin, particularly provide a kind of novel method improving turmeric saponin extract yield, belong to technical field of biochemical industry.
Background technology
Yellow ginger has another name called Rhizome of Peltate Yam, be Dioscoreaceae Wild yam (
dioscrea.L) per nnial herb is the kind that Determination of Diosgenin is the highest in the world.Sapogenin is the important foundation raw material producing steroid hormone class medicine.Steroid hormone has anti-infective, antianaphylaxis, antiviral and Antishock function.
At present, the treatment process report about improving yield in the production technique of turmeric saponin is more.Such as, Tong Ling etc. adopt enzymolysis pre fermentation process yellow ginger, the final saponin yield of turmeric saponin on average can reach 2.37%, has raising by a relatively large margin can improve (technical study that the enzymolysis process such as Shaanxi Normal University's journal 20030601 Tong Ling extracts diosgenin) compared with the yield 1.84% of direct acid-hydrolysis method.Domestic application patent " a kind of cleaning manufacturing technique method of turmeric saponin " (application number 201310144472.X) describes a kind of method of direct pre fermentation process, 45-60 DEG C of spontaneous fermentation 48-72h process, and turmeric saponin yield also significantly improves; In addition, some heat are extracted and high pressure homogenization method technique also shows that the yield of saponin increases (colleges and universities' chemical engineering journal, the turmeric saponin novel technology for extracting researchs such as 20110402 Pan He woodss).Although above these report methods all higher amplitude improve the yield of turmeric saponin.But the yield of turmeric saponin still has very large room for promotion, in order to improve yield more significantly.The present invention, by lot of experiments in early stage, to temperature and pH value synergic adjustment, significantly improves pre fermentation effect, thus efficiently obtains turmeric saponin.
Summary of the invention
The object of the present invention is to provide a kind of processing method improving turmeric saponin extract yield.This processing method is the feature according to endogenous enzyme various in yellow ginger cell, temperature section is adopted to control in the pre fermentation stage that turmeric saponin extracts, in conjunction with the coordinately regulated method of pH, ensure that various endogenous enzyme is had an effect, give full play to various endogenous enzyme effect during the fermentation, reach the object improving turmeric saponin extract yield, reduce the yellow ginger consumption of unit saponin product, reduce the discharge of waste, enhance productivity, reduce production cost.
In order to realize above-mentioned object, the present invention once by lot of experiments research also persistent exploration, finally obtained following technical scheme:
Improve a method for turmeric saponin extract yield, it is characterized in that: comprise the following steps:
(1) yellow ginger cleaned and pulverize, obtained crushed material;
(2) adopt Discrete control temperature fermentation, constant pH 5 ~ 6, is characterized in that: temperature section controls 42 DEG C of fermentations 6 hours, is then warming up to 55 DEG C of fermentations 12 hours, then is warming up to 68 DEG C of fermentations 6 hours, and the pre fermentation time is 24 hours;
(3) by fermentation after crushed material proceed to hydrolysis bottle in carry out acid hydrolysis, hydrolyzate is separated;
(4) isolated hydrolyzate is adjusted to neutrality, then hydrolyzate is dried, obtained just extract;
(5) adopt gasoline or sherwood oil to extract primary extract, after being dried by extract, namely obtain turmeric saponin.
Preferably, a kind of method improving turmeric saponin extract yield as above, is characterized in that: comprise the following steps:
(1) yellow ginger cleaned and pulverize, obtained crushed material;
(2) adopt and sectional temperature-controlledly to ferment in conjunction with pH synergic adjustment, it is characterized in that: when pre fermentation starts, temperature is 42 DEG C, ferment 6 hours in pH4.5 ~ 5.5, then be warming up to 55 DEG C, pH3.5 ~ 4.5 ferment 12 hours, be warming up to again 68 DEG C, pH3.5 ~ 3.0 ferment 6 hours, the pre fermentation time is 24 hours;
(3) by fermentation after crushed material proceed to hydrolysis bottle in carry out acid hydrolysis, hydrolyzate is separated;
(4) isolated hydrolyzate is adjusted to neutrality, then hydrolyzate is dried, obtained just extract;
(5) adopt gasoline or sherwood oil to extract primary extract, after being dried by extract, namely obtain turmeric saponin.
Preferably, a kind of method improving turmeric saponin extract yield as above, is characterized in that: the pretreated method of yellow ginger is that the ratio being 1:2 ~ 3 in the mass ratio of cadmium yellow ginger and water adds water and is crushed to particle diameter 2-5mm by after cadmium yellow ginger cleaning removal of impurities.
Compared with prior art, the technology of the present invention has verified single Discrete control temperature, constant pH be 5 ~ 6 method and two-stage temperature control in conjunction with the coordinately regulated method of pH on the impact of turmeric saponin extract yield, determine two-stage temperature control in conjunction with the coordinately regulated method of pH.
The method technical process provided in the present invention is simple, energy consumption is low, and the yield of turmeric saponin, compared with the yield of turmeric saponin in existing pre fermentation technique, improves more than 30%.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is described further, but protection scope of the present invention is not limited to these embodiments.Every do not deviate from the present invention's design change or equivalent substituting include within scope.
embodiment 1
First, inquire into the pretreatment process of cadmium yellow ginger, its process program is: after cadmium yellow ginger cleaning removal of impurities, the ratio being 1:2 ~ 3 in the mass ratio of cadmium yellow ginger and water adds water and is crushed to particle diameter 2-5mm.
Secondly, inquired into single Discrete control temperature, the method for constant pH 5 ~ 6 and two-stage temperature control are in conjunction with the coordinately regulated method of pH on the impact of turmeric saponin extract yield, and its concrete grammar is as follows:
Get 4200 grams of cadmium yellow ginger, after cadmium yellow ginger cleaning removal of impurities, the ratio being 1:2 ~ 3 in the mass ratio of cadmium yellow ginger and water adds water and is crushed to particle diameter 2-5mm, and it is evenly divided into 21 equal portions; Carry out three groups of tests, often organize test seven parallel.Taking-up crushed material after each parallel test pre fermentation completes, proceeds to the crushed material after fermentation in hydrolysis bottle and carries out acid hydrolysis, be separated by hydrolyzate; Isolated hydrolyzate is adjusted to neutrality, then hydrolyzate is dried, obtained primary extract; Adopt gasoline or sherwood oil to extract primary extract, after being dried by extract, namely obtain turmeric saponin.The technological method of the extract yield of turmeric saponin removes its maximum and maximum value to the turmeric saponin yield of seven parallel tests, and remaining five parallel averages.
According to above-mentioned embodiment, testing program and result as follows:
Test group one (is numbered No. 1, test) as a control group, and test conditions is: at 70 DEG C, pre fermentation 24 hours under the condition of pH5 ~ 6.
Test group two is testing program one (being numbered No. 2, test), is single Discrete control temperature, constant pH 5 ~ 6.Test conditions is: 42 DEG C ferment 6 hours, is then warming up to 55 DEG C of fermentations 12 hours, then is warming up to 68 DEG C of fermentations 6 hours, and pH is constant is 5 ~ 6.
Test group three is testing program two (being numbered No. 3, test), for two-stage temperature control is in conjunction with pH synergic adjustment.Test conditions is: 42 DEG C, pH4.5 ~ 5.5 ferment 6 hours, be then warming up to 55 DEG C, pH3.5 ~ 4.5 ferment 12 hours, then be warming up to 68 DEG C, pH3.5 ~ 3.0 ferment 6 hours.
Through overtesting and calculating, in each group, the yield of turmeric saponin the results are shown in Table 1.
table 1 pre fermentation mode is on the impact of turmeric saponin yield
Can learn from upper table, adopt single Discrete control temperature, constant pH is 5 ~ 6 pre fermentation methods of 24 hours, and under the condition of the final yield of its turmeric saponin and existing 70 DEG C, pH5 ~ 6, pre fermentation improves about 15% in 24 hours; Adopt two-stage temperature control in conjunction with the pH synergic adjustment pre fermentation method of 24 hours, under the condition of the final yield of its turmeric saponin and existing 70 DEG C, pH5 ~ 6, pre fermentation improves more than 30% in 24 hours.
Claims (3)
1. improve a method for turmeric saponin extract yield, it is characterized in that: comprise the following steps:
(1) yellow ginger cleaned and pulverize, obtained crushed material;
(2) adopt Discrete control temperature fermentation, constant pH 5 ~ 6, is characterized in that: temperature section controls 42 DEG C of fermentations 6 hours, is then warming up to 55 DEG C of fermentations 12 hours, then is warming up to 68 DEG C of fermentations 6 hours, and the pre fermentation time is 24 hours;
(3) by fermentation after crushed material proceed to hydrolysis bottle in carry out acid hydrolysis, hydrolyzate is separated;
(4) isolated hydrolyzate is adjusted to neutrality, then hydrolyzate is dried, obtained just extract;
(5) adopt gasoline or sherwood oil to extract primary extract, after being dried by extract, namely obtain turmeric saponin.
2. a kind of method improving turmeric saponin extract yield as claimed in claim 1, is characterized in that: comprise the following steps:
(1) yellow ginger cleaned and pulverize, obtained crushed material;
(2) adopt and sectional temperature-controlledly to ferment in conjunction with pH synergic adjustment, it is characterized in that: when pre fermentation starts, temperature is 42 DEG C, ferment 6 hours in pH4.5 ~ 5.5, then be warming up to 55 DEG C, pH3.5 ~ 4.5 ferment 12 hours, be warming up to again 68 DEG C, pH3.5 ~ 3.0 ferment 6 hours, the pre fermentation time is 24 hours;
(3) by fermentation after crushed material proceed to hydrolysis bottle in carry out acid hydrolysis, hydrolyzate is separated;
(4) isolated hydrolyzate is adjusted to neutrality, then hydrolyzate is dried, obtained just extract;
(5) adopt gasoline or sherwood oil to extract primary extract, after being dried by extract, namely obtain turmeric saponin.
3. a kind of method improving turmeric saponin extract yield described in claim 1 or 2, is characterized in that: the pretreated method of yellow ginger is that the ratio being 1:2 ~ 3 in the mass ratio of cadmium yellow ginger and water adds water and is crushed to particle diameter 2-5mm by after cadmium yellow ginger cleaning removal of impurities.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110904181A (en) * | 2019-11-20 | 2020-03-24 | 华南农业大学 | Method for improving saponin yield of Dioscorea composita by natural fermentation |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560239A (en) * | 2009-05-25 | 2009-10-21 | 北京大学 | Production process for extracting saponins from yellow ginger by using solvent catalyzing method |
| CN101575358A (en) * | 2009-05-25 | 2009-11-11 | 北京大学 | Method for extracting saponin from hydrolysate with high saponin content |
| CN102464898A (en) * | 2010-11-13 | 2012-05-23 | 陕西理工学院 | Method for recovering yellow ginger pigment from yellow ginger hydrolysate saponin extraction waste liquid |
| CN103059095A (en) * | 2013-01-07 | 2013-04-24 | 李玉山 | Green extraction technology for comprehensive and efficient utilization of Dioscorea zingiberensis resource |
| CN103882087A (en) * | 2014-04-22 | 2014-06-25 | 中国地质大学（武汉） | Rapid fermentation production method of yam diosgenin |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101560239A (en) * | 2009-05-25 | 2009-10-21 | 北京大学 | Production process for extracting saponins from yellow ginger by using solvent catalyzing method |
| CN101575358A (en) * | 2009-05-25 | 2009-11-11 | 北京大学 | Method for extracting saponin from hydrolysate with high saponin content |
| CN102464898A (en) * | 2010-11-13 | 2012-05-23 | 陕西理工学院 | Method for recovering yellow ginger pigment from yellow ginger hydrolysate saponin extraction waste liquid |
| CN103059095A (en) * | 2013-01-07 | 2013-04-24 | 李玉山 | Green extraction technology for comprehensive and efficient utilization of Dioscorea zingiberensis resource |
| CN103882087A (en) * | 2014-04-22 | 2014-06-25 | 中国地质大学（武汉） | Rapid fermentation production method of yam diosgenin |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110904181A (en) * | 2019-11-20 | 2020-03-24 | 华南农业大学 | Method for improving saponin yield of Dioscorea composita by natural fermentation |
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