CN105029624A - Lycium ruthenicum Murr. beverage and preparing method thereof - Google Patents

Lycium ruthenicum Murr. beverage and preparing method thereof Download PDF

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Publication number
CN105029624A
CN105029624A CN201510561610.3A CN201510561610A CN105029624A CN 105029624 A CN105029624 A CN 105029624A CN 201510561610 A CN201510561610 A CN 201510561610A CN 105029624 A CN105029624 A CN 105029624A
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extract
black fruit
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马菊秀
任蓓蕾
雷占兰
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Qinghai Zhiye Technology Consulting Service Co Ltd
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Qinghai Zhiye Technology Consulting Service Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages

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Abstract

The invention relates to a Lycium ruthenicum Murr. beverage. Through combination of Lycium ruthenicum Murr. extracts with an antioxidant function, grape seed extracts with an antioxidant function, and coriolus mushroom extracts with an antioxidant function, an excellent antioxidant effect is realized. Meanwhile, the method and process for preparing the Lycium ruthenicum Murr. beverage are simple, easy to operate and capable of facilitating industrialized production.

Description

A kind of black fruit lyceum beverage and preparation method thereof
Technical field
The present invention relates to field of food, be specifically related to a kind of black fruit lyceum beverage.
Background technology
Black fruit lyceum (LyciumruthenicumMurr.), fruit black, another name is " Soviet Union matrimony vine ", Tibetan medicine claims " other agate ", anaesthetic claims " Qiao Nuoying-Ha Er mug(unit of measure) ", for Solanaceae Lycium, many quils, multi-branched, branch is hard in white or canescence, the irregular vertical stripe of tool, sprig top becomes echinate, and leaf 2-6 piece clusters on brachyplast, and 1-2, flower is born on brachyplast, berry is spherical, in atropurpureus (including mauve pigment) after ripe, slightly cave in top, diameter 4mm-9mm; Highly general at 20cm-50cm, flowering fruit bearing stage is in 5-10 month; Seed is kidney shape, brown.Perennial salt tolerant, the drought resisting wild barbarum resource of new excavation in recent years, be distributed in Ningxia, Gansu, Qinghai, the Inner Mongol, Xinjiang, Tibet and North Shaanxi loess plateau, also there is distribution in the areas such as the Central Asia, Caucasia and Europe, its ripe berry is black, be rich in anthocyanin class pigment, be different from matrimony vine, therefore locality is referred to as black fruit lyceum, among the people often eaten raw as wild fruit or squeezed the juice do red drink and eat, also in its fruit useful, contained pigment makes dyeing.
Effective medicinal components and the lycium barbarum of black fruit lyceum are close.Modern study finds that lycium ruthenicum fruit is containing a certain amount of essential fatty acid, alkaloid, phenols, protein, vitamin, and containing 18 kinds of essential amino acids, its trace element is also very abundant, in addition containing more reduction polysaccharide and colors, especially containing abundant natural procyanidins, the title of the king that custom has " OPC ".Black fruit lyceum has higher medical value and nutritive value as can be seen here, research finds, its active ingredient mainly comprises the following aspects: (1) nutritional labeling: the crude fat of black fruit lyceum is about 6%, and main fatty acid has 3 kinds, mostly is unrighted acid; Black fruit lyceum contains 17 seed amino acids, and wherein content of glutamic acid is the highest, is secondly asparatate, methionine and cystine minimum.Protein content is about about 11%, and total reducing sugar is about 34%, and content of reducing sugar is about 33%, in addition, has also been rich in multivitamin E etc.; (2) polyphenols such as anthocyanin; (3) Flavonoid substances: be rich in Flavonoid substances in lycium ruthenicum fruit and leaf, Flavonoid substances in fruit is mainly anthocyanin, but the flavones in leaf is different from the flavones in fruit, consists predominantly of rutin, Quercetin, cyanidenon, Isorhamnetin and Kaempferol; (4) OPC.
About the pharmacological action of black fruit lyceum, " brilliant pearl draft ", the Four-Volume Medical Code are recorded, and Tibetan medicine is used as medicine with its ripening fruits, the illnesss such as treatment cardiopyretic disease, heart disease, irregular menstruation, menelipsis." Uygur medicine will " is recorded, the diseases such as lycium ruthenicum fruit and the treatment of root micromicro urethral calculus, tinea scabies, gingiva bleeding gingival hemorrhage, among the people then as strengthening by means of tonics, improving eyesight and depressor.Modern study finds, its pharmacologically active relates generally to the following aspects: (1) is hypoglycemic, antifatigue, the effect of raising immunity of organisms: polysaccharide has good regulating action and is confirmed by a large amount of research institute, and also there is the multiple physiologically active such as raising hypoxia-bearing capability, enhancing immunity, much research shows, black fruit lyceum and lycium ruthenicum polysaccharide have a lot of immunoloregulation function, comprise the activation of various immunocyte, traditional Chinese medical science current research shows, and lycium ruthenicum polysaccharide can strengthen cell and get involved immunity and humoral immune reaction; (2) anti-oxidant anti-aging effect: the ripe berry of black fruit lyceum is rich in abundant pigment polyphenol substance, mauve pigment and OPC etc., the test of many research workers all demonstrates anthocyanin and has scavenging free radicals and oxidation resistant function, OPC has anti-oxidant, scavenging free radicals, and its oxidation resistance is 50 times of vitamin E; (3) anticancer antitumor action: research finds that lycium ruthenicum polysaccharide and pigment polyphenol also have certain prevention and resistant function to tumour and cancer, found by clinical and experimental study, OPC can prevent the carcinoma of the rectum, and preventative higher than colon cancer to the carcinoma of the rectum.
Grape pip has very high nutritive value and medical value, and wherein contained OPC is one of important component of grape seed biological activity.Grape pip procyanidin is the polymer be connected to form by C4-C6 or C4-C8 key covalency by catechin, epicatechin and gallate thereof, in molecule due to this kind of material, there is electron rich hydroxylic moiety, making it have the ability of stronger anti-oxidant, scavenging free radicals and anti-lipid peroxidation, is the most potent one of the Astragaloside Ⅲ and lipid peroxidation inhibitor that find at present.
Rainbow conk (Coriolusversicolor) be a kind of preciousness of Polyporaceae Coriolus Qu61 medicine food with variegated bacterium, be also called Coriolus Versicolor, Coriolus, thousand layers of mushroom, bright cloud leather lid bacterium etc., be born in the dry wood of multiple broad leaf tree, fall on the live standing tree of wood, deadwood and aging, also be born on the rotten wood of the coniferous tree such as larch, black pine, mostly be wild.Rainbow conk is one of fungi of most medical value, wherein containing various bioactivators such as polysaccharide, protein, polypeptide, amino acid, purpurin, terpenes, have heat-clearing, removing toxic substances, anti-inflammatory, anticancer, the effect such as to protect the liver, medical value is extensive, is is researched and developed into health drink.Active component in rainbow conk is various, coriolan is the principle active component in rainbow conk, coriolan is proteinaceous glycopeptide, main component is with β-(1,4), β-(1,3) or β-(Isosorbide-5-Nitrae), β-(1,6) be main chain, on have β-(1,3) or the branch that combines of β-(1,6), polyoses content 30%-60% in its glycopeptide, protein content 10%-30%, monose forms mainly glucose, is secondly mannose, a small amount of galactolipin, wood sugar and fucose.Coriolan has Tumor suppression, antiulcer, protects the liver, antiviral, reducing blood lipid, anti-ageing multiple pharmacological effect of waiting for a long time, toxic and side effect is little, is widely used in clinical.Coriolan (CVPS) is to two class Major Enzymes in Active oxygen eliminating enzyme system, namely SOD and the sweet peroxidase of paddy Guang (GSH-Px) have obvious humidification, effectively can improve the ability of body opposing Damage Induced by Reactive Oxygen Species, there is antioxidation activity in stronger body.
The health food comprising black fruit lyceum, grape pip or rainbow conk in prior art is varied, but health food three share there is not yet.Thus, how to make full use of the nutritive value of black fruit lyceum, grape pip and rainbow conk, exploitation has the new product of excellent health-care efficacy, and meeting and enrich the growing material requisite of people, is field of health care food problem demanding prompt solution.
Summary of the invention
The object of the invention is to overcome above-mentioned defect, provide a kind of new black fruit lyceum beverage, it for main active, is equipped with the grape seed extract and Coriolus Versicolor P.E. equally with anti-oxidation efficacy with black fruit lyceum extract, has excellent antioxygenic property.
The technical scheme that the present invention solves this technical problem is:
A kind of black fruit lyceum beverage, its raw material consists of: black fruit lyceum extract, grape seed extract, Coriolus Versicolor P.E., syrup and pure water, wherein, the weight proportion of black fruit lyceum extract, grape seed extract and Coriolus Versicolor P.E. is 5-10%:5-10%:5-10%.
The preparation method of described black fruit lyceum extract is: get fresh lycium ruthenicum fruit 100g, is placed in tool plug conical flask, adds volume fraction 95% ethanol 1000mL, ultrasonic process 1h, let cool, supply 95% ethanol, centrifugal in 4000rpm, get supernatant and filter, concentrated, dry, to obtain final product.
The preparation method of described grape seed extract is: get grape pip 100g, also dry through petroleum ether degreasing after pulverizing, join in 60% ethanol by solid-liquid ratio 1:10, extract 2h in 60 DEG C of water bath with thermostatic control agitating heating, be cooled to 20 DEG C, centrifugal 30min under 3000rpm condition, repeatedly extract three times, merge supernatant, decompression distillation, drying, to obtain final product.
The preparation method of described Coriolus Versicolor P.E. is: accurately take Yunzhi powder 20g, adds 500mL distilled water with after 95% alcohol degreasing, ultrasonic extraction 1h, suction filtration, filtrate reduced in volume, with 95% ethanol alcohol precipitation of 2 times of volumes, filter, will precipitate drying, add water redissolution, with sevage method removing protein, again add absolute ethyl alcohol alcohol precipitation, filter, precipitation petroleum ether, drying, to obtain final product.
The preparation method of described syrup is: by white granulated sugar 40g, sucrose 30g, oligoisomaltose 30g, orange flavor 2, is added in 300mL, the pure water of 100 DEG C, boil 10 minutes, after stirring, be filled in storage syrup can by thick juice filter, cooling, to obtain final product.
Meanwhile, present invention also offers a kind of preparation method of black fruit lyceum beverage, its operating procedure is:
(1) preparation of black fruit lyceum extract: accurately take black fruit lyceum 100g and pulverized 60 mesh sieves, extracts 3 times by solid-liquid ratio 1:20 adding distil water, each 1.5h, filter, merging filtrate, reduced pressure concentration, with the 95% ethanol alcohol precipitation 3 times of 2 times of volumes, filter, be deposited in 40 DEG C of dryings, add water redissolution, adds sevage reagent removing protein 15 times in 1: 1 ratio, and solution adds absolute ethyl alcohol alcohol precipitation 3 times, filter, precipitation uses benzinum, acetone cyclic washing respectively, in 40 DEG C of dryings, to obtain final product;
(2) preparation of grape seed extract: get grape pip 100g, also dry through petroleum ether degreasing after pulverizing, join in 60% ethanol by solid-liquid ratio 1:10, extract 2h in 60 DEG C of water bath with thermostatic control agitating heating, be cooled to 20 DEG C, centrifugal 30min under 3000rpm condition, repeatedly extract three times, merge supernatant, decompression distillation, drying, to obtain final product;
(3) preparation of Coriolus Versicolor P.E.: accurately take Yunzhi powder 20g, adds 500mL distilled water with after 95% alcohol degreasing, ultrasonic extraction 1h, suction filtration, filtrate reduced in volume, with 95% ethanol alcohol precipitation of 2 times of volumes, filter, will precipitate drying, add water redissolution, with sevage method removing protein, again add absolute ethyl alcohol alcohol precipitation, filter, precipitation petroleum ether, drying, to obtain final product;
(4) syrup preparation: by white granulated sugar 40g, sucrose 30g, oligoisomaltose 30g, orange flavor 2 is added dropwise to in 300mL, the pure water of 100 DEG C, boils 10 minutes, after stirring, be filled in storage syrup can by thick juice filter, cooling, to obtain final product;
(5) according to the total weight of beverage, add pure water after preferably black fruit lyceum extract, grape seed extract and Coriolus Versicolor P.E. being mixed with syrup according to the proportioning of 5-10%:5-10%:5-10% and be stirred to whole dissolving, filling, sterilizing, to obtain final product.
Beneficial effect of the present invention:
(1) the present invention by by all having the black fruit lyceum extract of anti-oxidation function, grape pip extract and Coriolus Versicolor P.E. with the use of, create excellent antioxidation.
(2) black fruit lyceum method for preparing beverage technique of the present invention is simple, is easy to operation, is conducive to realizing industrialized production and processing.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, the usually conveniently conditioned disjunction condition of advising according to manufacturer.Unless otherwise indicated, otherwise all percentage, ratio, ratio or number by weight.
Unless otherwise defined, all specialties used in literary composition and scientific words and one skilled in the art the meaning be familiar with identical.In addition, any method similar or impartial to described content and material all can be applicable in the inventive method.The use that better implementation method described in literary composition and material only present a demonstration.
Embodiment 1:
(1) preparation of black fruit lyceum extract: accurately take black fruit lyceum 100g and pulverized 60 mesh sieves, extracts 3 times by solid-liquid ratio 1:20 adding distil water, each 1.5h, filter, merging filtrate, reduced pressure concentration, with the 95% ethanol alcohol precipitation 3 times of 2 times of volumes, filter, be deposited in 40 DEG C of dryings, add water redissolution, adds sevage reagent removing protein 15 times in 1: 1 ratio, and solution adds absolute ethyl alcohol alcohol precipitation 3 times, filter, precipitation uses benzinum, acetone cyclic washing respectively, in 40 DEG C of dryings, to obtain final product;
(2) preparation of grape seed extract: get grape pip 100g, also dry through petroleum ether degreasing after pulverizing, join in 60% ethanol by solid-liquid ratio 1:10, extract 2h in 60 DEG C of water bath with thermostatic control agitating heating, be cooled to 20 DEG C, centrifugal 30min under 3000rpm condition, repeatedly extract three times, merge supernatant, decompression distillation, drying, to obtain final product;
(3) preparation of Coriolus Versicolor P.E.: accurately take Yunzhi powder 20g, adds 500mL distilled water with after 95% alcohol degreasing, ultrasonic extraction 1h, suction filtration, filtrate reduced in volume, with 95% ethanol alcohol precipitation of 2 times of volumes, filter, will precipitate drying, add water redissolution, with sevage method removing protein, again add absolute ethyl alcohol alcohol precipitation, filter, precipitation petroleum ether, drying, to obtain final product;
(4) syrup preparation: by white granulated sugar 40g, sucrose 30g, oligoisomaltose 30g, orange flavor 2 is added dropwise to in 300mL, the pure water of 100 DEG C, boils 10 minutes, after stirring, be filled in storage syrup can by thick juice filter, cooling, to obtain final product;
(5) according to the total weight of beverage, add pure water after black fruit lyceum extract, grape seed extract and Coriolus Versicolor P.E. being mixed with syrup according to the proportional quantity of 10%:10%:10% and be stirred to whole dissolving, filling, sterilizing, to obtain final product.
Embodiment 2:
(1) preparation of black fruit lyceum extract: accurately take black fruit lyceum 100g and pulverized 60 mesh sieves, extracts 3 times by solid-liquid ratio 1:20 adding distil water, each 1.5h, filter, merging filtrate, reduced pressure concentration, with the 95% ethanol alcohol precipitation 3 times of 2 times of volumes, filter, be deposited in 40 DEG C of dryings, add water redissolution, adds sevage reagent removing protein 15 times in 1: 1 ratio, and solution adds absolute ethyl alcohol alcohol precipitation 3 times, filter, precipitation uses benzinum, acetone cyclic washing respectively, in 40 DEG C of dryings, to obtain final product;
(2) preparation of grape seed extract: get grape pip 100g, also dry through petroleum ether degreasing after pulverizing, join in 60% ethanol by solid-liquid ratio 1:10, extract 2h in 60 DEG C of water bath with thermostatic control agitating heating, be cooled to 20 DEG C, centrifugal 30min under 3000rpm condition, repeatedly extract three times, merge supernatant, decompression distillation, drying, to obtain final product;
(3) preparation of Coriolus Versicolor P.E.: accurately take Yunzhi powder 20g, adds 500mL distilled water with after 95% alcohol degreasing, ultrasonic extraction 1h, suction filtration, filtrate reduced in volume, with 95% ethanol alcohol precipitation of 2 times of volumes, filter, will precipitate drying, add water redissolution, with sevage method removing protein, again add absolute ethyl alcohol alcohol precipitation, filter, precipitation petroleum ether, drying, to obtain final product;
(4) syrup preparation: by white granulated sugar 40g, sucrose 30g, oligoisomaltose 30g, orange flavor 2 is added dropwise to in 300mL, the pure water of 100 DEG C, boils 10 minutes, after stirring, be filled in storage syrup can by thick juice filter, cooling, to obtain final product;
(5) according to the total weight of beverage, add pure water after black fruit lyceum extract, grape seed extract and Coriolus Versicolor P.E. being mixed with syrup according to the proportional quantity of 5%:5%:5% and be stirred to whole dissolving, filling, sterilizing, to obtain final product.
Embodiment 3:
(1) preparation of black fruit lyceum extract: accurately take black fruit lyceum 100g and pulverized 60 mesh sieves, extracts 3 times by solid-liquid ratio 1:20 adding distil water, each 1.5h, filter, merging filtrate, reduced pressure concentration, with the 95% ethanol alcohol precipitation 3 times of 2 times of volumes, filter, be deposited in 40 DEG C of dryings, add water redissolution, adds sevage reagent removing protein 15 times in 1: 1 ratio, and solution adds absolute ethyl alcohol alcohol precipitation 3 times, filter, precipitation uses benzinum, acetone cyclic washing respectively, in 40 DEG C of dryings, to obtain final product;
(2) preparation of grape seed extract: get grape pip 100g, also dry through petroleum ether degreasing after pulverizing, join in 60% ethanol by solid-liquid ratio 1:10, extract 2h in 60 DEG C of water bath with thermostatic control agitating heating, be cooled to 20 DEG C, centrifugal 30min under 3000rpm condition, repeatedly extract three times, merge supernatant, decompression distillation, drying, to obtain final product;
(3) preparation of Coriolus Versicolor P.E.: accurately take Yunzhi powder 20g, adds 500mL distilled water with after 95% alcohol degreasing, ultrasonic extraction 1h, suction filtration, filtrate reduced in volume, with 95% ethanol alcohol precipitation of 2 times of volumes, filter, will precipitate drying, add water redissolution, with sevage method removing protein, again add absolute ethyl alcohol alcohol precipitation, filter, precipitation petroleum ether, drying, to obtain final product;
(4) syrup preparation: by white granulated sugar 40g, sucrose 30g, oligoisomaltose 30g, orange flavor 2 is added dropwise to in 300mL, the pure water of 100 DEG C, boils 10 minutes, after stirring, be filled in storage syrup can by thick juice filter, cooling, to obtain final product;
(5) according to the total weight of beverage, add pure water after black fruit lyceum extract, grape seed extract and Coriolus Versicolor P.E. being mixed with syrup according to the proportional quantity of 8%:8%:8% and be stirred to whole dissolving, filling, sterilizing, to obtain final product.
Embodiment 4: the antioxidation in vitro efficacy test of black fruit lyceum beverage
Measure by improving one's methods of Smirnoff, use FeSO 4and H 2o 2reaction produce OH, with the light absorption value of OH oxidize water poplar acid sodium products therefrom represent OH number, light absorption value is larger, shows that OH is more.Containing 0.15mol/LFeSO in 3mL reactant liquor 4, 6mmol/LH 2o 2, 2mmol/L sodium salicylate and the black fruit lyceum beverage as test solution embodiment 1-3, replace black fruit lyceum beverage for contrast with distilled water, finally add H 2o 2start reaction and react 1h under 37 DEG C of conditions.Survey the absorbance value A at 510nm place, obtain the inhibiting rate to OH.Result is as shown in table 1.
Inhibiting rate=(A contrasts-A sample)/A contrasts × 100%
A contrast, A sample represent contrast liquid and the reacted light absorption value of test solution respectively
Table 1: the mensuration of the scavenging free radicals effect of black fruit lyceum beverage
Metering A510nm Clearance rate (%)
Control group 0.35±0.032 --
Embodiment 1 0.21±0.004a 41.12
Embodiment 2 0.23±0.003a 35.27
Embodiment 3 0.22±0.004a 38.14
Result shows, and black fruit lyceum beverage of the present invention has significant radical scavenging activity, and antioxidation is remarkable.
Embodiment 5: meet following quality index according to the product that embodiment 1-3 obtains:
(1) organoleptic indicator
Flavour and smell: distinctive delicate fragrance fragrance, coordinate soft, moderately sour and sweet, free from extraneous odour and bitter taste;
Tissue morphology: the transparency liquid of uniformity, no suspended substance, without layering, without precipitation;
Impurity: without macroscopic impurity and precipitation.
(2) microbiological indicator
Total plate count≤100cfu/mL
Coliform≤3MPN/100mL
Pathogenic bacteria (salmonella, Shigella, staphylococcus aureus): do not detect.
(3) physical and chemical index
Soluble solid (refractometry, 20 DEG C of refractometers) >=5.0%
Total acid (acid-base titration, with citrometer) 2.0g/L
Arsenic (in As)≤0.2mg/kg
Plumbous (in Pb)≤0.3mg/kg
Copper (in Cu)≤0.5mg/kg
The foregoing is only preferred embodiment of the present invention, and be not used to limit substantial technological context of the present invention, substantial technological content of the present invention is broadly defined in the right of application, any technology entities that other people complete or method, if with application right define identical, also or a kind of change of equivalence, be all covered by being regarded as among this right.

Claims (6)

1. a black fruit lyceum beverage, it is characterized in that, its raw material consists of: black fruit lyceum extract, grape seed extract, Coriolus Versicolor P.E., syrup and pure water, wherein, the weight proportion of black fruit lyceum extract, grape seed extract and Coriolus Versicolor P.E. is 5-10%:5-10%:5-10%.
2. black fruit lyceum beverage according to claim 1, is characterized in that, the preparation method of described black fruit lyceum extract is: get fresh lycium ruthenicum fruit 100g, be placed in tool plug conical flask, add volume fraction 95% ethanol 1000mL, ultrasonic process 1h, lets cool, supply 95% ethanol, centrifugal in 4000rpm, get supernatant and filter, concentrated, drying, to obtain final product.
3. black fruit lyceum beverage according to claim 1, is characterized in that, the preparation method of described grape seed extract is: get grape pip 100g, also dry through petroleum ether degreasing after pulverizing, join in 60% ethanol by solid-liquid ratio 1:10, extract 2h in 60 DEG C of water bath with thermostatic control agitating heating, be cooled to 20 DEG C, centrifugal 30min under 3000rpm condition, repeatedly extract three times, merge supernatant, decompression distillation, drying, to obtain final product.
4. black fruit lyceum beverage according to claim 1, is characterized in that, the preparation method of described Coriolus Versicolor P.E. is: accurately take Yunzhi powder 20g, 500mL distilled water is added, ultrasonic extraction 1h, suction filtration with after 95% alcohol degreasing, filtrate reduced in volume, with 95% ethanol alcohol precipitation of 2 times of volumes, filters, to precipitate drying, add water redissolution, with sevage method removing protein, again add absolute ethyl alcohol alcohol precipitation, filter, precipitation petroleum ether, drying, to obtain final product.
5. black fruit lyceum beverage according to claim 1, it is characterized in that, the preparation method of described syrup is: by white granulated sugar 40g, sucrose 30g, oligoisomaltose 30g, orange flavor 2, is added in 300mL, the pure water of 100 DEG C, boils 10 minutes, after stirring, be filled in storage syrup can by thick juice filter, cooling, to obtain final product.
6. a preparation method for black fruit lyceum beverage, is characterized in that, its operating procedure is:
(1) preparation of black fruit lyceum extract: get fresh lycium ruthenicum fruit 100g, is placed in tool plug conical flask, adds volume fraction 95% ethanol 1000mL, ultrasonic process 1h, let cool, supply 95% ethanol, centrifugal in 4000rpm, get supernatant and filter, concentrated, dry, to obtain final product;
(2) preparation of grape seed extract: get grape pip 100g, also dry through petroleum ether degreasing after pulverizing, join in 60% ethanol by solid-liquid ratio 1:10, extract 2h in 60 DEG C of water bath with thermostatic control agitating heating, be cooled to 20 DEG C, centrifugal 30min under 3000rpm condition, repeatedly extract three times, merge supernatant, decompression distillation, drying, to obtain final product;
(3) preparation of Coriolus Versicolor P.E.: accurately take Yunzhi powder 20g, adds 500mL distilled water with after 95% alcohol degreasing, ultrasonic extraction 1h, suction filtration, filtrate reduced in volume, with 95% ethanol alcohol precipitation of 2 times of volumes, filter, will precipitate drying, add water redissolution, with sevage method removing protein, again add absolute ethyl alcohol alcohol precipitation, filter, precipitation petroleum ether, drying, to obtain final product;
(4) syrup preparation: by white granulated sugar 40g, sucrose 30g, oligoisomaltose 30g, orange flavor 2, is added in 300mL, the pure water of 100 DEG C, boil 10 minutes, after stirring, be filled in storage syrup can by thick juice filter, cooling, to obtain final product;
(5) according to the total weight of beverage, add pure water after preferably black fruit lyceum extract, grape seed extract and Coriolus Versicolor P.E. being mixed with syrup according to the proportioning of 5-10%:5-10%:5-10% and be stirred to whole dissolving, filling, sterilizing, to obtain final product.
CN201510561610.3A 2015-09-06 2015-09-06 Lycium ruthenicum Murr. beverage and preparing method thereof Pending CN105029624A (en)

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Application publication date: 20151111