CN105021741A - Analytical method and apparatus for bionic absorption liquid used for main stream smoke - Google Patents
Analytical method and apparatus for bionic absorption liquid used for main stream smoke Download PDFInfo
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Abstract
The invention discloses an analytical method and apparatus for a bionic absorption liquid used for main stream smoke. The objective of the invention is to provide the method and apparatus with good analysis accuracy and comprehensive analysis capacity for analysis of the bionic absorption liquid used for main stream smoke. The analytical method comprises the following steps: connection of the analytical apparatus; setting of suction parameters; setting of external ambient temperature; analysis operation; and composition analysis of extract obtained after analysis operation. The analytical apparatus comprises a smoke absorption bottle, a simulated artificial oral mucosa inner wall, a saliva spray mechanism connected with an artificial saliva sprinkler pipe, a stirring adsorption rod, a magnetic stirrer arranged at the lower end of the smoke absorption bottle and in driving connection with the stirring adsorption rod, and a smoke collector and a vacuum pump sequentially connected with a smoke discharge pipe. The analytical method and apparatus directly handle smoking experience of a cigarette product and can provide powerful technical support and development base for research and development of cigarette products and fundamental research like physiological sensation of smoke and smoking and health.
Description
Technical field
The present invention relates to cigarette smoke analysis technical field, especially relate to analytical approach and the device thereof of the bionical absorbing liquid of main flume.
Background technology
Cigarette product is also a kind of main dependence organoleptic analysis is main product pattern.Consumer is exactly that smoker experiences flue gas for the stimulation of sense organ and the extent of reaction in aspiration procedure for the sole criterion of the recognition and acceptance degree of cigarette product.Therefore cigarette enterprise is exactly while guaranteeing security in final purpose, for consumer provides ideal joyful suction sensory experience.In view of sensory experience is the important indicator affecting cigarette product acceptance level, tobacco business researchers are devoted to study tobacco chemistry always, smoke chemistries etc., to the effect of cigarette sense organ suction quality, relate to the subjects such as smoke of tobacco chemistry, sensory organ physiology, behaviouristics, psychophysics and fuzzy mathematics therebetween.And all research starting points are all the effects stimulated for human body based on flue gas, because cigarette smoking sensory experience acts on by the smoke chemistry system of complexity the combined reaction and experience that comparatively responsive human feeling's device (sense of taste, sense of smell, taste bud etc.) formed afterwards.Wherein the suction comfortableness of cigarette depends primarily on taste perception.
Current tobacco chemistry, the research that smoke chemistry is evaluated for sensory comfort is existing a lot, tobacco components mainly concentrates on the chemical compositions such as total reducing sugar, reducing sugar, total nitrogen, nicotine, organic acid for the influence research of sense organ comfort level, is also evaluated by fume indication and organoleptic indicator in research; Smoke chemistry composition mainly concentrates on the impact for sense organ comfort level such as nicotine, tar, organic acid, phenols, keto-aldehyde ester class etc. in the granule phase substance of cambridge filter trapping
]the people such as Zhu just find that the volatile acid such as acetic acid, 3 methylvaleric acid is for alleviating the stimulation of nicotine for people's sense of taste, the fragrance of balance flue gas has vital role, Armitage etc. then find that free nicotine has stronger lipophilicity, more easily penetrate mucous membrane of mouth and be absorbed by the body, therefore can produce larger strength, also relatively easily obtain the hobby of consumer; A large amount of documents then shows phenols can cause the harmful effects such as pungent, astringent taste and assorted taste for mouthfeel, is the negative effect factor for taste perception in flue gas; Zhu Baokun etc. analyze 42 of flue gas chemical index, and carry out relevant to 10 sense organ Comfort Evaluation indexs, discovery phenols only orthoresol has obvious effect to oral cavity spine, organic acid Linoleic acid has obviously contribution etc. to oral cavity burr, strength, and these researchs advance the structure of smoke chemistry composition and sensory evaluation system all greatly.
But in research process, researcher also slowly finds in the trapping and Absorption Study of Chemical Components in Cigarette Smoke, the modes such as the cambridge filter always adopted or simulation artificial saliva directly absorb, this is the actual aspiration procedure with people in fact: main flume is exposed in oral cavity, after the of short duration effect of oral mucosa, Partial Feature chemical composition is contacted by oral mucosa, absorption, dissolve, absorb, transform, thus cause the process of sensory experience etc. to have different, and the important chemical composition of this part is only core and the key that flue gas causes sense organ physiological reaction just, the depth & wideth studied this moieties can directly affect the sensory experience of research smoke components aspirates to(for) consumer.Therefore, main flume is carried out oral cavity human simulation and absorb the necessity had very, and it is also extremely important to carry out detailed analysis to the composition in the bionical absorbing liquid in oral cavity, set up smoke absorption liquid component analyzing method and develop and aspirate with smoker the new technology experienced and be associated, can carry out absorbing and trapping causing the chemical composition of the feature of physiological reaction, and directly suck impression with cigarette product and link up with mutually, can research and develop for cigarette product, the impression of flue gas physiology, the fundamental research such as smoking and health provide strong technical support and development foundation.
Summary of the invention
Instant invention overcomes shortcoming of the prior art, provide a kind of simple to operate, precision of analysis is strong and analyze the analytical approach of the bionical absorbing liquid of comprehensive main flume.Meanwhile, the invention provides the analytical equipment of the bionical absorbing liquid of a kind of main flume.
In order to solve the problems of the technologies described above, the present invention is achieved by the following technical solutions:
An analytical approach for the bionical absorbing liquid of main flume, it comprises the following steps:
A, cigarette is positioned over the end of smoke inlet pipe smoke absorption bottle with the first valve, air connecting pipe end smoke absorption bottle with the second valve is connected with smoking machine, and sets suitable pumping volume, puff interval and puff duration;
The simulation artificial oral-cavity mucous membrane inwall of the full artificial saliva of leaching is inserted in b, smoke absorption bottle;
C, the artificial saliva shower with saliva imported valve be placed into smoke absorption bottle top and fix, and connecting peristaltic pump, afterwards the pump orifice of peristaltic pump being stretched in artificial saliva reservoir;
D, the smoke exhaust pipe of tool exhaust valve to be connected with Fume collector, and vacuum pump is used for the discharge of flue gas on Fume collector end connects;
E, at the bottom of smoke absorption bottle, leave a small amount of artificial saliva, and put into the stirring and adsorbing rod that has magnetic core, for absorbing the related chemical constituents in saliva;
After f, total interface connect, guarantee the impermeability of junction, and be fixed on magnetic stirring apparatus by smoke absorption bottle, smoke absorption bottle and magnetic stirring apparatus are positioned in isoperibol, keep ambient temperature to be 37 DEG C ± 0,5 DEG C;
G, when analyzing beginning, the first valve and the second valve is kept to be in open mode, exhaust valve and saliva imported valve are in closed condition, flue gas is inhaled in smoke absorption bottle by smoking machine, and keep parking blink, open peristaltic pump and saliva imported valve afterwards, arranging flow velocity is 1mL/min, simulated saliva is secreted, artificial saliva pumps in reservoir, carry out simulation artificial oral-cavity mucous membrane inwall by artificial saliva shower to realize scrubbing and again infiltrating, open magnetic stirring apparatus simultaneously, the rotating speed arranging stirring and adsorbing rod is 800rmp, for the Related Component in on-line adsorption and the effective artificial saliva of flue gas,
After h, flue gas park blink, close the first valve, the second valve and saliva imported valve, open exhaust opening valve, and open vacuum pump, flue gas in smoke absorption bottle is pumped, is collected on Fume collector, after simulation human body smoking cigarette, tell the process of cigarette;
I, artificial saliva spray and smoke evacuation all complete in suction gap, repeat step g, h, until cigarette has been sucked;
After j, smoking terminate, keep stirring and adsorbing rod to continue extraction absorption 1h, after extraction terminates, take out stirring and adsorbing rod, utilize thermal desorption-gas chromatography tandem mass spectrum to carry out constituent analysis.
Preferably, described pumping volume is 35mL, and puff interval is 1min, and puff duration is 2s;
Preferably, be on-line extraction for the extraction of smoke absorption liquid Related Component, extract in flue gas aspiration procedure and occur simultaneously, and the assay by selecting stirring and adsorbing of different nature rod to be used for opposed polarity composition in the bionical absorbing liquid in oral cavity.
Preferably, described smoke absorption bottle and magnetic stirring apparatus are positioned in constant temperature oven, keep its external environment to be in temperature constant state by constant temperature oven.
An analytical equipment for the bionical absorbing liquid of main flume, it comprises:
Smoke absorption bottle, for simulating smoker's oral cavity; The bottom of described smoke absorption bottle arranges the smoke inlet pipe with the first valve, and top is provided with the air connecting pipe with the second valve and the smoke exhaust pipe with exhaust valve, and its top is provided with the artificial saliva shower with saliva imported valve;
Simulation artificial oral-cavity mucous membrane inwall, it to be arranged in smoke absorption bottle and the full artificial saliva of leaching, for the saliva liquid film of simulating smoker's oral cavity wall;
Connect the saliva spraying mechanism of artificial saliva shower, for simulating smoker's salivary secretion; Described saliva spraying mechanism comprises the peristaltic pump be connected with artificial saliva shower, and for holding the artificial saliva reservoir of artificial saliva; The pump orifice of described peristaltic pump stretches into the liquid level of artificial saliva reservoir by pipeline under;
Stirring and adsorbing rod, to be arranged in smoke absorption bottle and to be positioned at lower central place, for Related Component extraction absorption in smoke absorption liquid;
Be arranged at smoke absorption bottle lower end and drive with stirring and adsorbing rod the magnetic stirring apparatus be connected; And
Be linked in sequence the Fume collector of smoke exhaust pipe and vacuum pump successively.
Compared with prior art, tool of the present invention has the following advantages:
The bionical absorbing liquid analytical approach in main flume oral cavity of the present invention and device are just based on smoker's smoking-flue gas diffusion-smoke components absorption, the process that solution absorption-cause sensory experience-flue gas is discharged, devising and a kind of enter the intraoral bionical absorption plant of smoker for studying main flume: smoking machine smoking cigarette produces flue gas-flue gas and to enter in smoke absorption bottle-flue gas diffusion-smoke components and infiltrated the simulation artificial oral-cavity mucous membrane contact internal walls of artificial saliva, absorption, dissolving-flue gas is discharged, after the extraction absorption of smoke absorption liquid, may be used for cigarette mainstream flue gas dissolve or the Related Component acted in saliva carry out assay, and main flume can be acted on saliva part contrast with the flue gas of discharging during smoking, understand the change of cigarette smoking process chemistry composition in depth, the method structure is simple, easy to operate, the more important thing is the similar process because these apparatus and method and smoker aspirate, and by selecting stirring and adsorbing of different nature rod, the smoke components that extractive analysis is different, the method obtain the bionical absorbing liquid of main flume in composition and the cigarette smoking aesthetic quality degree of correlation higher, obtain analyzing data more closing to reality suction conditions by the method, more can reflect the quality of cigarette product.According to embodiment between the data obtained, the present invention directly sucks impression with cigarette product and links up with mutually, can research and develop, the impression of flue gas physiology, the fundamental research such as smoking and health provide strong technical support and development foundation for cigarette product.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation of analytical equipment in the present invention.
Fig. 2 is certain cigarette sample main flume oral cavity bionical absorbing liquid extracting apolar composition gas chromatogram.
Fig. 3 is the bionical absorbing liquid extraction in certain cigarette sample main flume oral cavity polar component gas chromatogram.
Fig. 4 is certain cigarette sample smoke evacuation flue gas trapping thing gas chromatogram.
Fig. 5 be the first brand cigarette after bionical absorption of the present invention, the nonpolarity element figure of extracting and enriching.
Fig. 6 be the second brand cigarette after bionical absorption of the present invention, the nonpolarity element figure of extracting and enriching.
Fig. 7 be the first brand cigarette after bionical absorption of the present invention, the polar component figure of extracting and enriching.
Fig. 8 be the second brand cigarette after bionical absorption of the present invention, the polar component figure of extracting and enriching.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not paying the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
The object of the invention is to the disappearance analyzed for the bionical absorption in current main flume oral cavity and absorbing liquid or deficiency, provide total analysis method and the bionical absorption plant of main flume of the bionical absorbing liquid of a kind of main flume.The method can carry out human simulation to main flume absorption process in the oral cavity during smoker's smoking cigarette, and carries out the extracting and enriching work of main flume absorbing liquid simultaneously, is convenient to carry out main flume and carries out in the oral cavity spreading, adsorb and dissolving.
Smoking consumer is when sucking cigarette product, the main flume of suction enters in human oral cavity, and carried out diffusion, have skim saliva due to the oral cavity of people and the surface adhesion of tongue, this layer of saliva has carried out absorption, dissolving and absorption to flue gas, the flue gas material that this part enters in saliva is experienced by the taste stimulation causing oral cavity and tongue surface after itself or biological conversion, and the correlation research that the chemical substance of this part causes smoker's sense organ physiology to experience for research cigarette smoke is most important.
The analytical equipment of the bionical absorbing liquid of main flume shown in Fig. 1, it comprises:
Smoke absorption bottle 6, for simulating smoker's oral cavity; The bottom of described smoke absorption bottle 6 arranges the smoke inlet pipe 2 with the first valve 3, top is provided with the air connecting pipe 4 with the second valve 5 and the smoke exhaust pipe 13 with exhaust valve 14, and its top is provided with the artificial saliva shower 8 with saliva imported valve 9;
Simulation artificial oral-cavity mucous membrane inwall 7, it to be arranged in smoke absorption bottle 6 and the full artificial saliva of leaching, for the saliva liquid film of simulating smoker's oral cavity wall;
Connect the saliva spraying mechanism of artificial saliva shower 8, for simulating smoker's salivary secretion; Described saliva spraying mechanism comprises the peristaltic pump 11 be connected with artificial saliva shower 8, and for holding the artificial saliva reservoir 12 of artificial saliva; The pump orifice of described peristaltic pump 11 stretches into the liquid level of artificial saliva reservoir 12 by pipeline under;
Stirring and adsorbing rod 18, to be arranged in smoke absorption bottle 6 and to be positioned at lower central place, for Related Component extraction absorption in smoke absorption liquid;
Be arranged at smoke absorption bottle 6 lower end and drive with stirring and adsorbing rod 18 magnetic stirring apparatus 19 be connected; And
Be linked in sequence the Fume collector 16 of smoke exhaust pipe 13 and vacuum pump 17 successively.
The analytical approach of the bionical absorbing liquid of main flume, it comprises the following steps:
A, cigarette 1 is positioned over the end of the smoke inlet pipe 2 smoke absorption bottle 6 with the first valve 3, air connecting pipe 4 end smoke absorption bottle 6 with the second valve 5 is connected with smoking machine, and set suitable pumping volume, puff interval and puff duration, preferably, described pumping volume is 35mL, puff interval is 1min, and puff duration is 2s;
B, in smoke absorption bottle 6, put into the simulation artificial oral-cavity mucous membrane inwall 7 of a full artificial saliva of leaching;
C, the artificial saliva shower 8 with saliva imported valve 9 be placed into smoke absorption bottle 6 top and fix, and connecting peristaltic pump 11, afterwards the pump orifice of peristaltic pump 11 being stretched in artificial saliva reservoir 12;
D, the smoke exhaust pipe 13 of tool exhaust valve 14 to be connected with Fume collector 16, and on Fume collector 16 end connects vacuum pump 17 for the discharge of flue gas;
E, leave a small amount of artificial saliva at smoke absorption bottle 6 end, and put into the stirring and adsorbing rod 18 that has magnetic core, for absorbing the related chemical constituents in saliva;
After f, total interface connect, guarantee the impermeability of junction, and smoke absorption bottle 6 is fixed on magnetic stirring apparatus 19, smoke absorption bottle 6 and magnetic stirring apparatus 19 are positioned in isoperibol, in such as Fig. 1, smoke absorption bottle 6 and magnetic stirring apparatus 19 are positioned in constant temperature oven 20, keep its external environment to be in temperature constant state by constant temperature oven, keep ambient temperature to be 37 DEG C ± 0,5 DEG C;
G, when analyzing beginning, the first valve 3 and the second valve 5 is kept to be in open mode, exhaust valve 14 and saliva imported valve 9 are in closed condition, flue gas is inhaled in smoke absorption bottle 6 by smoking machine, and keep parking blink, open peristaltic pump 11 and saliva imported valve 9 afterwards, arranging flow velocity is 1mL/min, simulated saliva is secreted, artificial saliva pumps in reservoir, carry out simulation artificial oral-cavity mucous membrane inwall 7 by artificial saliva shower 8 to realize scrubbing and again infiltrating, open magnetic stirring apparatus 19 simultaneously, the rotating speed arranging stirring and adsorbing rod 18 is 800rmp, for the Related Component in on-line adsorption and the effective artificial saliva of flue gas,
After h, flue gas park blink, close the first valve 3, second valve 5 and saliva imported valve 9, open exhaust opening valve, and open vacuum pump 17, flue gas in smoke absorption bottle 6 is pumped, is collected on Fume collector 16, after simulation human body smoking cigarette, tell the process of cigarette;
I, artificial saliva spray and smoke evacuation all complete in suction gap, repeat step g, h, until cigarette 1 has been sucked;
After j, smoking terminate, keep stirring and adsorbing rod 18 to continue extraction absorption 1h, after extraction terminates, take out stirring and adsorbing rod 18, utilize thermal desorption-gas chromatography tandem mass spectrum to carry out constituent analysis.
Wherein, be on-line extraction for the extraction of smoke absorption liquid Related Component, extract in flue gas aspiration procedure and occur simultaneously, and by selecting stirring and adsorbing rod 18 of different nature for the assay of opposed polarity composition in the bionical absorbing liquid in oral cavity.
Embodiment 1: based on the gas chromatography Tandem Mass Spectrometry Analysis method of composition (polar component, nonpolarity element) in the bionical absorbing liquid in main flume oral cavity of the present invention.
Certain cigarette sample is equilibrium moisture 48 h under temperature 22 DEG C ± 1 DEG C, relative humidity 60 ± 3% environment, filters out qualified cigarette, and in smoke absorption bottle, putting into a full artificial saliva of leaching, (formula is: NaCl 0.4 g/L, KCl 0.4 g/L, CaCl
22H
2o 0.795 g/L, NaH
2pO
42H
2o 0.78 g/L, Na
2s
.2H
2o 0.005 g/L, urea 1.0 g/L, pH=6.8 ± 0.5) simulation artificial oral-cavity mucous membrane inwall, and place a stirring and adsorbing rod at the bottom of smoke absorption bottle bottle, in artificial saliva reservoir, add artificial saliva, and connect saliva mouth spray and peristaltic pump, in Fume collector, put into a slice cambridge filter, and connect vacuum pump and Fume collector, be placed on magnetic stirring apparatus by the flue gas trapping bottle connecting each pipeline, and package unit is placed in constant temperature oven, temperature is set as 37 DEG C ± 0.5 DEG C, the bionical absorption experiment of main flume is carried out with standard smoking conditions (1 mouthful/min), pumping volume is 35 mL, puff interval is 1 min, puff duration is 2 s, open peristaltic pump in puff interval and carry out saliva spray, and open magnetic stirring apparatus, the rotating speed arranging stirring and adsorbing rod (18) is 800 rmp, for the Related Component in on-line adsorption and the effective artificial saliva of flue gas, during puff interval, after flue gas parks blink, vacuum pump (17) is utilized to be discharged by the unnecessary flue gas in smoke absorption bottle, and be captured in Fume collector (16), follow-up to be measured, after continuous sucking 2 cigarette, close artificial saliva mouth spray and peristaltic pump, keep stirring and adsorbing rod to continue absorption 1 h, taking-up stirring and adsorbing rod, utilizes the surface of a small amount of washed with de-ionized water adsorbing bar, and carries out drying, to treat that next step experiment is used.
Stirring and adsorbing rod after above-mentioned extraction process is put into thermal desorption-gas chromatography tandem mass spectrum and carries out Related Component desorption and stratographic analysis, according to stirring and adsorbing rod of different nature, extraction component of different nature can be analyzed.
1, nonpolarity element:
Thermal desorption instrument condition is: thermal desorption initial temperature is 40 DEG C, rises to 300 DEG C with 60 DEG C/min speed, keeps 5min; Cold-trap sampling condition is: initial temperature is-50 DEG C, rises to 300 DEG C with 720 DEG C/min speed, keeps 5min, the emptying mode sample introduction of solvent; Be interior mark with D-8 naphthalene, gas chromatography tandem mass spectrum instrument condition is: column temperature rises to 250 DEG C from 40 DEG C (keeping 5min) with the speed of 4 DEG C/min, then rises to 280 DEG C (keeping 5min) with the speed of 10 DEG C/min; Splitless injecting samples, carrier gas is high-purity helium, and flow velocity is 1mL/min, ionization pattern is electron impact ion source (EI), and ionization energy is 70eV, and image data mode is full scan pattern, quality of scanning scope is m/z35-450, adopts NIS98 standard diagram storehouse to carry out data retrieval.Fig. 2 be certain cigarette product after bionical absorption of the present invention, through the chromatogram of the extraction of nonpolar stirring and adsorbing rod and thermal desorption and stratographic analysis.
2, polar component:
Thermal desorption instrument condition is: thermal desorption initial temperature is 40 DEG C, rises to 220 DEG C with 60 DEG C/min speed, keeps 5min; Cold-trap sampling condition is: initial temperature is-100 DEG C, rises to 300 DEG C with 720 DEG C/min speed, keeps 5min, the emptying mode sample introduction of solvent; Be interior mark with phenylethyl propionate, gas chromatography tandem mass spectrum instrument condition is with 1.Fig. 3 be certain cigarette product after bionical absorption of the present invention, through the extraction of polarity stirring and adsorbing rod and the chromatogram of thermal desorption and stratographic analysis.
3, smoke evacuation trapping thing:
Tweezers are utilized to take out the cambridge filter in Fume collector, and put into clean triangular flask, accurately add 20mL methylene chloride, and utilize ultrasonic extraction 30min, after utilizing 0.45 μm of organic filter membrane to filter extract, clear liquid utilizes gas chromatography tandem mass spectrum to carry out auto injection analysis, and instrument condition is with 1.Fig. 4 is smoke evacuation flue gas trapping thing gas chromatogram.
The analysis of the bionical absorbing liquid Semi-polarity of flue gas and nonpolarity element, impression can be aspirated associate actual to flue gas practical function composition and cigarette, that really accomplishes that chemical composition experiences with physiology is associated, can for studying the key chemical components identification that cause physiology to experience and extraction provides theory support and technical support; And smoke evacuation trapping thing simulation be smoking cigarette time smoke evacuation process, the Main Function of these trapping things is cause the physiology of sense of smell to experience, the analysis of the trapping thing of this part not only can may be used for the change studying smoke components before and after cigarette smoking, also can provide analytical approach and means for causing the fume component analysis of nasal receptor.
Embodiment 2: based on the characteristic component analysis of the cigarette product of main flume of the present invention bionical absorption approach difference suction sensory experience.The difference that effect due to the heterogeneity in cigarette mainstream flue gas can cause the suction of cigarette to experience, and these difference if the suction of dependence people subjectivity trap relevant to cambridge filter, in fact differ greatly with actual conditions.
Characteristic component based on the cigarette product of difference suction sensory experience of the present invention analyzes detecting step: the two kinds of cigarette getting different suction sensory experience carry out the bionical absorption in main flume oral cavity, absorption process utilizes heterogeneity stirring and adsorbing rod to carry out extracting and enriching respectively, and carry out analyzing (analytical approach is with embodiment 1), Fig. 5 and Fig. 6 be two kinds of different brands cigarette after bionical absorption of the present invention, the nonpolarity element figure of extracting and enriching.Comparison diagram 5 and Fig. 6 known, the nonpolarity element of kinds of cigarettes product is basically identical, even if some trickle change profiles are also basically identical, this illustrates that the main nonpolarity element of kinds of cigarettes product is roughly the same, but within be designated as benchmark, find that the amount of some nonpolarity element of each cigarette product is that tool is discrepant, and these differences may be exactly cause sense organ to aspirate different keys; Fig. 7 and Fig. 8 is that different brands cigarette is after bionical absorption of the present invention, the polar component figure of extracting and enriching, pass through comparative analysis, can find that the contrast difference of polar component and nonpolarity element is larger, this is likely because polar component more easily acts in saliva, affect larger with the stimulus to the sense organ in oral cavity, more having correlativity for the analysis of these compositions and contrast with sensory experience, is also the good point of penetration of cigarette physiology impression from now on and the research work of smoke chemistry interaction between component.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. an analytical approach for the bionical absorbing liquid of main flume, is characterized in that comprising the following steps:
A, cigarette is positioned over the end of smoke inlet pipe smoke absorption bottle with the first valve, air connecting pipe end smoke absorption bottle with the second valve is connected with smoking machine, and sets suitable pumping volume, puff interval and puff duration;
The simulation artificial oral-cavity mucous membrane inwall of the full artificial saliva of leaching is inserted in b, smoke absorption bottle;
C, the artificial saliva shower with saliva imported valve be placed into smoke absorption bottle top and fix, and connecting peristaltic pump, afterwards the pump orifice of peristaltic pump being stretched in artificial saliva reservoir;
D, the smoke exhaust pipe of tool exhaust valve to be connected with Fume collector, and vacuum pump is used for the discharge of flue gas on Fume collector end connects;
E, at the bottom of smoke absorption bottle, leave a small amount of artificial saliva, and put into the stirring and adsorbing rod that has magnetic core, for absorbing the related chemical constituents in saliva;
After f, total interface connect, guarantee the impermeability of junction, and be fixed on magnetic stirring apparatus by smoke absorption bottle, smoke absorption bottle and magnetic stirring apparatus are positioned in isoperibol, keep ambient temperature to be 37 DEG C ± 0,5 DEG C;
G, when analyzing beginning, the first valve and the second valve is kept to be in open mode, exhaust valve and saliva imported valve are in closed condition, flue gas is inhaled in smoke absorption bottle by smoking machine, and keep parking blink, open peristaltic pump and saliva imported valve afterwards, arranging flow velocity is 1mL/min, simulated saliva is secreted, artificial saliva pumps in reservoir, carry out simulation artificial oral-cavity mucous membrane inwall by artificial saliva shower to realize scrubbing and again infiltrating, open magnetic stirring apparatus simultaneously, the rotating speed arranging stirring and adsorbing rod is 800rmp, for the Related Component in on-line adsorption and the effective artificial saliva of flue gas,
After h, flue gas park blink, close the first valve, the second valve and saliva imported valve, open exhaust opening valve, and open vacuum pump, flue gas in smoke absorption bottle is pumped, is collected on Fume collector, after simulation human body smoking cigarette, tell the process of cigarette;
I, artificial saliva spray and smoke evacuation all complete in suction gap, repeat step g, h, until cigarette has been sucked;
After j, smoking terminate, keep stirring and adsorbing rod to continue extraction absorption 1h, after extraction terminates, take out stirring and adsorbing rod, utilize thermal desorption-gas chromatography tandem mass spectrum to carry out constituent analysis.
2. the analytical approach of the bionical absorbing liquid of main flume according to claim 1, it is characterized in that: described pumping volume is 35mL, puff interval is 1min, and puff duration is 2s.
3. the bionical absorbing liquid analytical approach in main flume oral cavity according to claim 1, it is characterized in that: extract as on-line extraction for smoke absorption liquid Related Component, extract in flue gas aspiration procedure and occur simultaneously, and the assay by selecting stirring and adsorbing of different nature rod to be used for opposed polarity composition in the bionical absorbing liquid in oral cavity.
4. the bionical absorbing liquid analytical approach in main flume oral cavity according to claim 1, is characterized in that: described smoke absorption bottle and magnetic stirring apparatus are positioned in constant temperature oven, keeps its external environment to be in temperature constant state by constant temperature oven.
5. the bionical absorbing liquid analytical approach in main flume oral cavity according to claim 1, is characterized in that: shown artificial saliva formula is: NaCl 0.4 g/L, KCl 0.4 g/L, CaCl
22H
2o 0.795 g/L, NaH
2pO
42H
2o 0.78 g/L, Na
2s
.2H
2o 0.005 g/L, urea 1.0 g/L, its pH=6.8 ± 0.5.
6. an analytical equipment for the bionical absorbing liquid of main flume, is characterized in that comprising:
Smoke absorption bottle, for simulating smoker's oral cavity; The bottom of described smoke absorption bottle arranges the smoke inlet pipe with the first valve, and top is provided with the air connecting pipe with the second valve and the smoke exhaust pipe with exhaust valve, and its top is provided with the artificial saliva shower with saliva imported valve;
Simulation artificial oral-cavity mucous membrane inwall, it to be arranged in smoke absorption bottle and the full artificial saliva of leaching, for the saliva liquid film of simulating smoker's oral cavity wall;
Connect the saliva spraying mechanism of artificial saliva shower, for simulating smoker's salivary secretion; Described saliva spraying mechanism comprises the peristaltic pump be connected with artificial saliva shower, and for holding the artificial saliva reservoir of artificial saliva; The pump orifice of described peristaltic pump stretches into the liquid level of artificial saliva reservoir by pipeline under;
Stirring and adsorbing rod, to be arranged in smoke absorption bottle and to be positioned at lower central place, for Related Component extraction absorption in smoke absorption liquid;
Be arranged at smoke absorption bottle lower end and drive with stirring and adsorbing rod the magnetic stirring apparatus be connected; And
Be linked in sequence the Fume collector of smoke exhaust pipe and vacuum pump successively.
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| CN105548462A (en) * | 2015-11-24 | 2016-05-04 | 中国食品发酵工业研究院 | Apparatus for simulating release of flavor of liquid foods in oral cavity and retronasal olfaction, and use method thereof |
| CN106920456A (en) * | 2017-01-14 | 2017-07-04 | 云南中烟工业有限责任公司 | A kind of bionical absorption interactive device for simulating human body throat to main flume |
| CN107091767A (en) * | 2017-04-19 | 2017-08-25 | 青岛贞正分析仪器有限公司 | A kind of water pollutant SPME sampling thermal desorption method and device |
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| CN107436341B (en) * | 2017-07-05 | 2020-02-07 | 苏州大学 | Tobacco evaluation device |
| CN107764716A (en) * | 2017-10-21 | 2018-03-06 | 云南中烟工业有限责任公司 | A kind of detection method of cigarette smoke to cellular water Permeability |
| CN107764716B (en) * | 2017-10-21 | 2020-08-14 | 云南中烟工业有限责任公司 | Method for detecting influence of cigarette smoke on cell water permeability |
| CN110596288A (en) * | 2019-09-25 | 2019-12-20 | 上海烟草集团有限责任公司 | Freezing enrichment separation method for main stream smoke components in artificial saliva |
| CN115326991A (en) * | 2022-09-02 | 2022-11-11 | 上海烟草集团有限责任公司 | Method for analyzing cigarette unpacking smell |
| CN115932188A (en) * | 2023-03-15 | 2023-04-07 | 江南大学 | Cigarette mainstream smoke oral cavity bionic absorption device |
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