CN105001329A - Method for extracting animal antiserera - Google Patents
Method for extracting animal antiserera Download PDFInfo
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- CN105001329A CN105001329A CN201510474305.0A CN201510474305A CN105001329A CN 105001329 A CN105001329 A CN 105001329A CN 201510474305 A CN201510474305 A CN 201510474305A CN 105001329 A CN105001329 A CN 105001329A
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- serum
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- antiserum
- antisera
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Abstract
The invention provides a method for extracting animal antiserera. The method comprises the following steps that 1 arterial cannula is carried out on animals subjected to antigen immune to take blood; 2 blood is collected through a glass bottle, collection is stopped after a certain volume of blood is obtained; 3 collected blood is put into water bath in time, the blood is taken out in time after serum is separated; 4 the separated serum is absorbed in time, still standing is carried out, and the serum separated in the glass bottle is absorbed repeatedly. Compared with the prior art, the method for extracting animal antiserera is low in cost and simple in process, antiserum can be extracted stably, the blood taking capacity of unit weight, the serum amount in whole blood and serum amount in the unit weight can be improved remarkably, and the animal and the serum can be made full use of.
Description
Technical field
The present invention relates to a kind of animal antiserum extracting method, belong to antiserum(antisera) extractive technique field.
Background technology
In today of biotechnology fast development, the purposes of animal serum is more and more extensive.Some immune serum, special serum are worth very high, so extraction goat-anti serum of how getting twice the result with half the effort is one of very important problem of current technical field of biology.Existing a lot of sero-fast extracting method in prior art,, although effectively antiserum(antisera) can be extracted, still there are some problems in most employing venous blood sampling, as serum amount in per weight blood sampling volume, whole blood and per weight serum amount all lower, cause the waste of blood and serum.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the invention provides a kind of animal antiserum extracting method.
Technical scheme: for achieving the above object, the invention provides a kind of animal antiserum extracting method, comprises the following steps:
(1) to learn from else's experience the animal of antigen immune, got blood by arterial cannulation;
(2) collect blood with vial, stop collecting to certain volume;
(3) blood of collection is put into water-bath in time, serum takes out after separating out in time;
(4) draw the serum of separating out in time, and leave standstill, the serum of separating out in repeated multiple times absorption bottle.
As preferably, described step (1) medium sized artery intubate is bilateral carotid arteries intubate.
Preferred as another kind, in described step (2), vial is the vial of 250ml.
Preferred as another kind, the certain volume in described step (2) is 40-60ml.
Preferred as another kind, in described step (3), bath temperature is identical with blood sampling animal heat.
Preferred as another kind, the time of putting into water-bath in described step (3) is 1-2 hour.
Preferred as another kind, the condition left standstill in described step (4) is that room temperature leaves standstill.
Preferred as another kind, described animal is sheep.
Preferred as another kind, described antiserum(antisera) is goat-anti human apolipoprotein A1 antiserum(antisera), goat-anti human apolipoprotein b antiserum(antisera) or goat-anti hCRP antiserum(antisera)
Antiserum(antisera) extracting method of the present invention, first adopt arterial blood extracting, arterial pressure is higher than venous pressure, and the speed that blood flows out from artery is fast, and the blood flow volume of collection is many; Secondly, the blood flow volume of every bottle will be less than routine techniques, thus serum can be made to separate out fully, and clot is less, and wherein remaining serum can be fewer; Finally, the blood of collection is put in time the water-bath of body temperature identical with animal of taking a blood sample, be both conducive to serum and separated out in time, also can not the quality such as to tire of variable effect serum of Yin Wendu.
Beneficial effect: relative to prior art, animal antiserum extracting method of the present invention, cost is low, process is simple, extraction antiserum(antisera) can not only be stablized, the most important thing is to significantly improve serum amount and per weight serum amount in per weight blood sampling volume, whole blood, make full use of animal and blood.
Embodiment
Below in conjunction with embodiment, the present invention is further described.
Embodiment 1
Goat-anti human apolipoprotein A1 antiserum(antisera) extracting method:
(1) to learn from else's experience the sheep of remarkable Apolipoprotein A1 antigen immune, get blood by bilateral carotid arteries intubate;
(2) collect blood with 250ml vial, the blood flow volume of every bottle is 40ml;
(3) blood of collection is put in time the water-bath 1 hour of body temperature identical with animal of taking a blood sample, serum takes out after separating out in time;
(4) draw the serum of separating out in time, and room temperature leaves standstill, the serum of separating out in repeated multiple times absorption bottle.
Embodiment 2
Goat-anti human apolipoprotein A1 antiserum(antisera) extracting method:
(1) to learn from else's experience the sheep of remarkable Apolipoprotein A1 antigen immune, get blood by bilateral carotid arteries intubate;
(2) collect blood with 250ml vial, the blood flow volume of every bottle is 60ml;
(3) blood of collection is put in time the water-bath 2 hours of body temperature identical with animal of taking a blood sample, serum takes out after separating out in time;
(4) draw the serum of separating out in time, and room temperature leaves standstill, the serum of separating out in repeated multiple times absorption bottle.
Embodiment 3
Goat-anti human apolipoprotein A1 antiserum(antisera) extracting method:
(1) to learn from else's experience the sheep of remarkable Apolipoprotein A1 antigen immune, get blood by bilateral carotid arteries intubate;
(2) collect blood with 250ml vial, the blood flow volume of every bottle is 50ml;
(3) blood of collection is put in time the water-bath 1.5 hours of body temperature identical with animal of taking a blood sample, serum takes out after separating out in time;
(4) draw the serum of separating out in time, and room temperature leaves standstill, the serum of separating out in repeated multiple times absorption bottle.
Embodiment 4
Goat-anti human apolipoprotein b antiserum(antisera) extracting method:
(1) to learn from else's experience the sheep of human apolipoprotein b antigen immune, got blood by bilateral carotid arteries intubate;
(2) collect blood with 250ml vial, the blood flow volume of every bottle is 50ml;
(3) blood of collection is put in time the water-bath 1.5 hours of body temperature identical with animal of taking a blood sample, serum takes out after separating out in time;
(4) draw the serum of separating out in time, and room temperature leaves standstill, the serum of separating out in repeated multiple times absorption bottle.
Embodiment 5
Goat-anti hCRP antiserum(antisera) extracting method:
(1) to learn from else's experience the sheep of c reactive protein antigen immune, got blood by bilateral carotid arteries intubate;
(2) collect blood with 250ml vial, the blood flow volume of every bottle is 50ml;
(3) blood of collection is put in time the water-bath 1.5 hours of body temperature identical with animal of taking a blood sample, serum takes out after separating out in time;
(4) draw the serum of separating out in time, and room temperature leaves standstill, the serum of separating out in repeated multiple times absorption bottle.
Embodiment 6 (goat-anti human apolipoprotein A1 antiserum(antisera) general extraction methods)
Goat-anti human apolipoprotein A1 antiserum(antisera) extracting method:
(1) to learn from else's experience the sheep of remarkable Apolipoprotein A1 antigen immune, the bloodletting of Bilateral Cervical venous cannula;
(2) collect blood with 500ml vial, vial is filled as far as possible;
(3) blood of collection is put 37 DEG C of incubators after 1 hour, then place room temperature and treat that serum is separated out;
(4) after serum is separated out in a large number, with suction pipe, serum is drawn in centrifugal bottle, the red corpuscle of centrifugal segregation lower sediment, collects upper serum.
Embodiment 7 (goat-anti human apolipoprotein b antiserum(antisera) general extraction methods)
Extracting method, with embodiment 6, is goat-anti human apolipoprotein b antiserum(antisera) unlike antiserum(antisera).
Embodiment 8 (goat-anti hCRP antiserum(antisera) general extraction methods)
Extracting method, with embodiment 6, is goat-anti hCRP antiserum(antisera) unlike antiserum(antisera).
The each extracting method effect monitoring of experimental example
One, the detection of goat-anti human apolipoprotein A1 antiserum(antisera) extraction effect
Get same batch of sheep, carry out immunity according to identical ordinary method, after immunity completes, be divided into two groups, one group 10, extract antiserum(antisera) according to embodiment 6 (ordinary method) method; Another group 15, antiserum(antisera) is extracted according to embodiment 3 (the inventive method) method, adopt two immunization that expands to detect gained antiserum titre, the index of each sheep gained serum the results are shown in Table 1 (ordinary method) and table 2 (the inventive method).
The each index result of table 1 embodiment 6 (ordinary method) gained antiserum(antisera)
The each index result of table 2 embodiment 3 (the inventive method) gained antiserum(antisera)
Can be obtained by upper table 1 and table 2 result, produce goat-anti human apolipoprotein A1 antiserum(antisera) by the inventive method, per weight blood sampling volume can improve 2.93%, and in whole blood, serum amount can improve 6.11%, and per weight serum amount can improve 9.13%, and serum titer does not affect substantially.
Two, the detection of goat-anti human apolipoprotein b antiserum(antisera) extraction effect
Get same batch of sheep, carry out immunity according to identical ordinary method, after immunity completes, be divided into two groups, one group 10, extract antiserum(antisera) according to embodiment 7 (ordinary method) method; Another group 15, antiserum(antisera) is extracted according to embodiment 4 (the inventive method) method, adopt two immunization that expands to detect gained antiserum titre, the index of each sheep gained serum the results are shown in Table 3 (ordinary methods) and table 4 (the inventive method).
The each index result of table 3 embodiment 7 (ordinary method) gained antiserum(antisera)
The each index result of table 4 embodiment 4 (the inventive method) gained antiserum(antisera)
Can be obtained by upper table 3 and table 4 result, produce goat-anti human apolipoprotein b antiserum(antisera) by the inventive method, per weight blood sampling volume can improve 3.83%, and in whole blood, serum amount can improve 5.93%, and per weight serum amount can improve 9.62%, and serum titer does not affect substantially.
Three, the detection of goat-anti hCRP antiserum(antisera) extraction effect
Get same batch of goat, carry out immunity according to identical ordinary method, after immunity completes, be divided into two groups, one group 10, extract antiserum(antisera) according to embodiment 8 (ordinary method) method; Another group 15, antiserum(antisera) is extracted according to embodiment 5 (the inventive method) method, adopt two immunization that expands to detect gained antiserum titre, the index of each sheep gained serum the results are shown in Table 5 (ordinary methods) and table 6 (the inventive method).
The each index result of table 5 embodiment 8 (ordinary method) gained antiserum(antisera)
The each index result of table 6 embodiment 5 (the inventive method) gained antiserum(antisera)
Can be obtained by upper table 5 and table 6 result, produce goat-anti hCRP antiserum(antisera) by the inventive method, per weight blood sampling volume can improve 3.09%, and in whole blood, serum amount can improve 7.22%, and per weight serum amount can improve 10.48%, and serum titer does not affect substantially.
Claims (9)
1. an animal antiserum extracting method, is characterized in that, comprises the following steps:
(1) to learn from else's experience the animal of antigen immune, got blood by arterial cannulation;
(2) collect blood with vial, stop collecting to certain volume;
(3) blood of collection is put into water-bath in time, serum takes out after separating out in time;
(4) draw the serum of separating out in time, and leave standstill, the serum of separating out in repeated multiple times absorption bottle.
2. goat-anti serum extracting method according to claim 1, is characterized in that, described step (1) medium sized artery intubate is bilateral carotid arteries intubate.
3. goat-anti serum extracting method according to claim 1, is characterized in that, in described step (2), vial is the vial of 250ml.
4. goat-anti serum extracting method according to claim 1, is characterized in that, the certain volume in described step (2) is 40-60ml.
5. goat-anti serum extracting method according to claim 1, is characterized in that, in described step (3), bath temperature is identical with blood sampling animal heat.
6. goat-anti serum extracting method according to claim 1, is characterized in that, the time of putting into water-bath in described step (3) is 1-2 hour.
7. goat-anti serum extracting method according to claim 1, is characterized in that, the condition left standstill in described step (4) is that room temperature leaves standstill.
8. goat-anti serum extracting method according to claim 1, is characterized in that, described animal is sheep.
9. goat-anti serum extracting method according to claim 1, is characterized in that, described antiserum(antisera) is goat-anti human apolipoprotein A1 antiserum(antisera), goat-anti human apolipoprotein b antiserum(antisera) or goat-anti hCRP antiserum(antisera).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510474305.0A CN105001329A (en) | 2015-08-05 | 2015-08-05 | Method for extracting animal antiserera |
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| CN201510474305.0A CN105001329A (en) | 2015-08-05 | 2015-08-05 | Method for extracting animal antiserera |
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| CN105001329A true CN105001329A (en) | 2015-10-28 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111574624A (en) * | 2020-05-06 | 2020-08-25 | 安徽省争华食品有限公司 | Hu sheep serum extraction process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017075A (en) * | 2014-01-06 | 2014-09-03 | 宁波博泰生物技术有限公司 | Method for preparing apolipoprotein B antiserum |
| CN104017076A (en) * | 2014-01-06 | 2014-09-03 | 宁波博泰生物技术有限公司 | Preparation method of apolipoprotein AI antiserum |
| CN104086653A (en) * | 2014-07-17 | 2014-10-08 | 中华人民共和国连云港出入境检验检疫局 | Preparation method and application of EHNV (Epizootic Haematopoietic Necrosis Virus) antiserum |
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- 2015-08-05 CN CN201510474305.0A patent/CN105001329A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017075A (en) * | 2014-01-06 | 2014-09-03 | 宁波博泰生物技术有限公司 | Method for preparing apolipoprotein B antiserum |
| CN104017076A (en) * | 2014-01-06 | 2014-09-03 | 宁波博泰生物技术有限公司 | Preparation method of apolipoprotein AI antiserum |
| CN104086653A (en) * | 2014-07-17 | 2014-10-08 | 中华人民共和国连云港出入境检验检疫局 | Preparation method and application of EHNV (Epizootic Haematopoietic Necrosis Virus) antiserum |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111574624A (en) * | 2020-05-06 | 2020-08-25 | 安徽省争华食品有限公司 | Hu sheep serum extraction process |
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Application publication date: 20151028 |