CN104998868A - Test tube cleaning line for cell culture - Google Patents

Test tube cleaning line for cell culture Download PDF

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Publication number
CN104998868A
CN104998868A CN201510413884.8A CN201510413884A CN104998868A CN 104998868 A CN104998868 A CN 104998868A CN 201510413884 A CN201510413884 A CN 201510413884A CN 104998868 A CN104998868 A CN 104998868A
Authority
CN
China
Prior art keywords
test tube
movable block
gear
bearing
motor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201510413884.8A
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Chinese (zh)
Inventor
单秋丽
马炎
鲍洁
张晓慧
方成成
赵芳
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Jinan
Original Assignee
University of Jinan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Jinan filed Critical University of Jinan
Priority to CN201510413884.8A priority Critical patent/CN104998868A/en
Publication of CN104998868A publication Critical patent/CN104998868A/en
Pending legal-status Critical Current

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00Cleaning hollow articles by methods or apparatus specially adapted thereto 
    • B08B9/08Cleaning containers, e.g. tanks
    • B08B9/087Cleaning containers, e.g. tanks by methods involving the use of tools, e.g. brushes, scrapers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00Cleaning hollow articles by methods or apparatus specially adapted thereto 
    • B08B9/08Cleaning containers, e.g. tanks
    • B08B9/093Cleaning containers, e.g. tanks by the force of jets or sprays

Abstract

A test tube cleaning line for cell culture is used for effectively cleaning test tubes. A test tube disc is arranged on the top of a base, the test tube disc is provided with a plurality grooves and guide grooves, a moving block is arranged in each guide groove in a sliding manner, a spring is arranged between each moving block and the test tube disc and the moving blocks have the tendency of moving to the center of the test tube disc under the action of the springs; an extending rod is arranged at the driving end of each moving block, the center of the test tube disc is provided with a rotary wheel driven by a first motor to rotate and action blocks are arranged at the outer wall of the rotary wheel; the action blocks contact with the extending rods and extrude the extending rods to move to one side away from the center of the test tube disc; and a hairbrush disc is provided with a plurality of outer gears, and a hairbrush is fixed on each outer gear axle. A washing assembly for washing the test tubes is arranged at the back side of the hairbrush disc, and comprises a water tank, a water pump, a nozzle disc and a water outlet pipe. The test tube cleaning line can realize brushing and washing of the test tubes and is high in cleaning efficiency.

Description

Cell chulture test tube cleans line
Technical field
The present invention relates to test tube cleaning technique field, specifically a kind of cleaning line of cell chulture test tube.
Background technology
Cell culture technology is also cell clone technology, and the regular noun in biology is cell culture technology.No matter for whole biotechnology, or the biological cloning technology of one of them, cell chulture is all a requisite process, the extensive clone of cell chulture inherently cell.Cell culture technology can be become the many cells of simply unicellular or few differentiation through mass propgation by a cell, this is the requisite link of clone technology, and the clone of cell chulture inherently cell.A large amount of cells or its metabolite is obtained by cell chulture.Because biological product is all get from cell, so can say that cell culture technology is most crucial in biotechnology, most basic technology.In cell chulture, what often use is test tube, and after having carried out cell chulture, the cleaning of test tube is a loaded down with trivial details job.
Summary of the invention
A kind of cell chulture test tube is the object of the present invention is to provide to clean line, for realizing the effective cleaning to test tube, to alleviate the manual labor of people.
The present invention solves the technical scheme that its technical problem takes: cell chulture test tube cleans line, it is characterized in that, comprise base, test tube clamping assembly, hairbrush assembly, first translation assembly, second translation assembly and washing assembly, test tube clamping assembly is provided with in one end of described base top, described test tube clamping assembly comprises the first bearing, test tube dish, movable block, runner, first motor and extension rod, described test tube dish is fixed on and is positioned on the first bearing of base left end, described test tube dish is circumferentially evenly provided with some grooves, some gathering sills circumferentially evenly arranged are provided with in the inside of the circumference at described groove place, described gathering sill is identical with the number of groove, and described gathering sill and corresponding groove are positioned in same radial direction, movable block is slidably fitted with in gathering sill described in each, spring is provided with between described movable block and test tube dish, the oriented trend near the movement of test tube disk center of movable block under the effect of described spring, described in each, the drive end of movable block is provided with an extension rod, is provided with by the runner of the first motor-driven rotation at the center of described test tube dish, and the outer wall of described runner is provided with some effect blocks, some described effect blocks and extension rod one_to_one corresponding, described effect block contacts with extension rod and extrolling boom when moving to extreme position to the side away from test tube disk center place, and the semicircle orifice at described movable block top and groove surround the circular clamp chamber of holding test tubes,
Described hairbrush assembly comprises the second bearing, brush plate, external gear, gear ring, internal gear and the second motor, described brush plate is fixed on and is positioned on the second bearing of base right-hand member, described brush plate is rotatablely equipped with some external gears circumferentially evenly arranged, described in each external gear gear shaft on be fixed with hairbrush, the number of described hairbrush is identical with the number of movable block; Some described external gears be positioned at a gear ring outside and with gear ring engagement fit, be provided with in the inside of described gear ring by the internal gear of the second motor-driven rotation, described internal gear and gear ring engagement fit;
Described washing assembly comprises three abutments, water tank and water pump, outlet pipe, spray nozzle plate and nozzle, described spray nozzle plate is fixed on the three abutments that is positioned on rear side of the first bearing, described spray nozzle plate is provided with some nozzles circumferentially evenly arranged, some described nozzles and test tube one_to_one corresponding; Be provided with water tank and water pump at the rear side of described base, described water tank and water pump are provided with outlet pipe, described outlet pipe is communicated with some described nozzles;
Be provided with on the right side of described base driving second bearing near or away from the first translation assembly of the first bearing;
Described chassis left side be provided with driving three abutments near or away from the second translation assembly of the first bearing.
Further, described first translation assembly comprises the first tooth bar, the first gear and the 3rd motor, described first tooth bar is fixed on the bottom of the second bearing and the first tooth bar is connected with base sliding, described first pinion rotation to be arranged on base and described first gear by the 3rd motor-driven rotation, described first tooth bar coordinates with the first gears meshing.
Further, described second translation assembly comprises the second tooth bar, the second gear and the 4th motor, described second tooth bar is fixed on the bottom of the first bearing and the second tooth bar is connected with base sliding, described second pinion rotation to be arranged on base and described second gear by the 4th motor-driven rotation, described second tooth bar coordinates with the second gears meshing.
Further, described in each, the both sides of movable block are respectively equipped with otic placode, and the test tube dish of described movable block both sides is provided with fixed block, and described spring is arranged between fixed block and corresponding otic placode.
Further, described test tube dish and brush plate are working of plastics.
Further, be provided with circular hole at the center of described test tube dish, described runner is placed in circular hole.
Further, described test tube dish is provided with draw-in groove, and in described draw-in groove, snap-fit engagement has the end cap covered on movable block.
Further, in the semicircle orifice of described groove and movable block, rubber blanket is equipped with.
The invention has the beneficial effects as follows: cell chulture test tube provided by the invention cleans line, the clamping to test tube is realized by test tube clamping assembly, by translation assembly make hairbrush assembly near or away from test tube clamping assembly, make hairbrush extend in test tube, when hairbrush rotates, can scrubbing inboard wall of test tube be realized.During cleaning inboard wall of test tube, by the rotation of driven by motor hairbrush, avoid free-hand problem of carrying out wasting time and energy when test tube cleans.Hairbrush is rinsed inboard wall of test tube by washing assembly after scrubbing test tube.Can remove the foreign material of inboard wall of test tube by scrubbing and rinse two procedures, operating efficiency is high.
Accompanying drawing explanation
Fig. 1 is schematic diagram of the present invention;
Fig. 2 is the schematic diagram of test tube clamping assembly;
Fig. 3 is the driving schematic diagram of runner;
The action schematic diagram of runner when Fig. 4 is holding test tubes;
Fig. 5 is the schematic diagram of hairbrush assembly;
Fig. 6 is the driving schematic diagram of the second bearing;
Fig. 7 is the driving schematic diagram of the first bearing;
Fig. 8 is the schematic diagram of washing assembly;
Fig. 9 is that A in Fig. 8 is to local direction view;
In figure: 1 base, 11 chutes, 2 end caps, 21 first bearings, 22 second bearings, 23 three abutments, 3 test tube dishes, 30 circular holes, 31 draw-in grooves, 32 grooves, 33 gathering sills, 34 movable blocks, 341 otic placodes, 35 extension rods, 351 inclined-planes, 36 fixed blocks, 37 runners, 371 runner shafts, 38 effect blocks, 39 springs, 4 brush plates, 41 external gears, 42 hairbrush, 43 gear rings, 44 internal gears, 5 clamping chambeies, 51 first motors, 52 second motors, 53 the 3rd motors, 54 the 4th motors, 61 first tooth bars, 62 internal gears, 71 second tooth bars, 72 second gears, 8 water tanks and water pump, 81 outlet pipes, 9 spray nozzle plates, 91 nozzles.
Detailed description of the invention
Below in conjunction with accompanying drawing, present invention is described.
As shown in Figure 1, base 1 is matrix of the present invention, the first bearing 21 is provided with in the left side of base top, first bearing is support component, first bearing is fixed with the circular and vertical test tube dish 3 placed, test tube dish is working of plastics, as shown in Figure 2, on test tube dish, circumferentially spaced set has some grooves 32, test tube dish is circumferentially gone back spaced set and has some gathering sills 33, the number of some gathering sills is identical with the number of groove, gathering sill and groove one_to_one corresponding, and gathering sill is between the groove and test tube disk center of correspondence.A movable block 34 is slidably fitted with in each gathering sill, an otic placode 341 is respectively equipped with in the both sides of each movable block, a fixed block 36 is also respectively equipped with in the both sides of each movable block, fixed block is fixed on test tube dish, the fixed block of each movable block both sides is corresponding with the otic placode of these movable block both sides respectively to be arranged, spring 39 is provided with between fixed block and corresponding otic placode, under the action of the spring, the trend of the center movement of the oriented test tube dish of movable block, is provided with semicircle orifice at the effect end of each movable block.Be provided with extension rod 35 at the drive end of each movable block, the installation end of extension rod is fixed on movable block, is provided with the inclined-plane 351 of inclination at the drive end of each extension rod.Be provided with circular hole 30 at the center of test tube dish, the drive end of each extension rod all extend in circular hole 30.
As shown in Figure 2, an end face of test tube dish is fixed with the first motor 51, as shown in Figure 3, runner shaft 371 is provided with at the first motor output end, runner shaft is fixed with runner 37, runner is placed in circular hole, and the outer wall of runner is provided with some effect blocks 38 circumferentially evenly arranged, and effect block is provided with arc surface.The number of effect block is identical with the number of extension rod, and the effect of effect block contacts mating reaction with extrolling boom, movable block to be moved with extension rod to the side away from test tube disk center, and moving radially along test tube dish when movable block moves.
As shown in Figure 4, when the first driven by motor runner rotates counterclockwise, effect block rotates along with runner, acts on the arc surface on block and the contact of incline plane on corresponding extension rod, along with the continuous rotation of runner, effect block extrolling boom makes extension rod radially outward move along test tube dish.Drive movable block outwards movement when extension rod moves, until when runner turns over certain angle, movable block radially moves to greatest limit position, and the semicircle orifice now on movable block and respective slot surround the clamping chamber 5 of holding test tubes.Can holding test tubes in a groove, then drive the first motor action, make movable block and the effect of test tube dish clamp test tube.First bearing, test tube dish, movable block, extension rod, runner and the first motor constitute test tube clamping assembly.For realizing the covering to movable block, test tube dish is provided with draw-in groove 31, and on draw-in groove, snap-fit engagement has end cap 2.
As shown in Figure 5, hairbrush assembly comprises the second support 22, brush plate 4, external gear 41, hairbrush 42, gear ring 43, internal gear 44 and the second motor 52, brush plate is circular working of plastics and is fixed on the second bearing, an end face of brush plate is provided with some external gears 41 circumferentially evenly arranged, and the number of external gear is identical with the number of movable block.The gear shaft of each external gear is fixed with hairbrush 42, and the brush holder of hairbrush is fixed on the gear shaft of external gear, around the brush holder of hairbrush, be provided with bristle.Brush plate is rotatablely equipped with gear ring 43, the inner side of gear ring and outer wall are equipped with tooth, tooth outside gear ring and some external gear engagement fit, internal gear 44 is provided with inside gear ring, tooth engagement fit inside internal gear and gear ring, internal gear is fixed on the second motor 52 output shaft, and the second motor is fixed on brush plate.When second motor exports rotary motion, drive the rotation of hairbrush by internal gear, gear ring and external gear.Now hairbrush is placed in test tube, can scrubbing inboard wall of test tube be realized.
As shown in Figure 6, for drive hairbrush assembly and test tube clamp assembly close to each other or away from, be fixed with the first tooth bar 61 in the bottom of the second bearing, base is provided with chute 11, and tooth bar is slidably mounted in chute.Base is rotatablely equipped with the first gear 62, first gear and drives rotation by the 3rd motor 53, the 3rd motor export rotary motion time, just can drive the first pinion rotation, so drive the first tooth bar move, make hairbrush assembly near or away from test tube clamping assembly.Drive the 3rd motor to export rotary motion, make the second bearing near the first bearing, enter into test tube to make hairbrush.3rd motor, the first gear and the first tooth bar constitute the first translation assembly.For reducing clamping chamber to the chucking power of test tube, preventing clamping chamber from pressing from both sides bad test tube, in the semicircle orifice of groove and movable block, being equipped with rubber blanket.
As shown in Figure 8, Figure 9, the base on rear side of hairbrush assembly is provided with water tank and water pump 8, water tank and water pump are provided with outlet pipe 81.Base on rear side of the second bearing is fixed with three abutments 23, three abutments is fixed with spray nozzle plate 9, and spray nozzle plate is circular working of plastics, and spray nozzle plate is vertically placed, spray nozzle plate is provided with some nozzles 91 circumferentially evenly arranged, and the number of nozzle is identical with the number of movable block.Some nozzles are connected with outlet pipe, and the cleaning fluid in outlet pipe or running water can enter into each nozzle, and hydraulic pressure on some nozzles is equal.The water sprayed in nozzle enters into corresponding test tube, to realize the flushing to inboard wall of test tube.Three abutments, water tank and water pump, outlet pipe, spray nozzle plate and nozzle constitute washing assembly.
As shown in Figure 7, the second tooth bar 71 is fixed with in the bottom of the first bearing, second tooth bar is connected with base sliding, be provided with the 4th motor 54 and the second gear 72 in the base, second gear is fixed on the output of the 4th motor, second gear and the second tooth bar engagement fit, when the 4th motor exports rotary motion, the movable of the first bearing can be driven by the second tooth bar and the second gear, when side moves to certain position to the first bearing backward, some test tubes are just to nozzle, and now can open water pump, running water just enters into test tube through outlet pipe.4th motor, the second tooth bar and the second gear constitute the second translation assembly.For realizing the control to the 4th electric machine operation state, be respectively equipped with travel switch in the front and back extreme position of the first bearing, when the first bearing contacts with front side travel switch, the 4th motor stops action, and test tube is just to hairbrush; When first bearing contacts with rear side travel switch, the 4th motor stops action, and test tube is just to nozzle.

Claims (8)

1. cell chulture test tube cleans line, it is characterized in that, comprise base, test tube clamping assembly, hairbrush assembly, first translation assembly, second translation assembly and washing assembly, test tube clamping assembly is provided with in one end of described base top, described test tube clamping assembly comprises the first bearing, test tube dish, movable block, runner, first motor and extension rod, described test tube dish is fixed on and is positioned on the first bearing of base left end, described test tube dish is circumferentially evenly provided with some grooves, some gathering sills circumferentially evenly arranged are provided with in the inside of the circumference at described groove place, described gathering sill is identical with the number of groove, and described gathering sill and corresponding groove are positioned in same radial direction, movable block is slidably fitted with in gathering sill described in each, spring is provided with between described movable block and test tube dish, the oriented trend near the movement of test tube disk center of movable block under the effect of described spring, described in each, the drive end of movable block is provided with an extension rod, is provided with by the runner of the first motor-driven rotation at the center of described test tube dish, and the outer wall of described runner is provided with some effect blocks, some described effect blocks and extension rod one_to_one corresponding, described effect block contacts with extension rod and extrolling boom when moving to extreme position to the side away from test tube disk center place, and the semicircle orifice at described movable block top and groove surround the circular clamp chamber of holding test tubes,
Described hairbrush assembly comprises the second bearing, brush plate, external gear, gear ring, internal gear and the second motor, described brush plate is fixed on and is positioned on the second bearing of base right-hand member, described brush plate is rotatablely equipped with some external gears circumferentially evenly arranged, described in each external gear gear shaft on be fixed with hairbrush, the number of described hairbrush is identical with the number of movable block; Some described external gears be positioned at a gear ring outside and with gear ring engagement fit, be provided with in the inside of described gear ring by the internal gear of the second motor-driven rotation, described internal gear and gear ring engagement fit;
Described washing assembly comprises three abutments, water tank and water pump, outlet pipe, spray nozzle plate and nozzle, described spray nozzle plate is fixed on the three abutments that is positioned on rear side of the first bearing, described spray nozzle plate is provided with some nozzles circumferentially evenly arranged, some described nozzles and test tube one_to_one corresponding; Be provided with water tank and water pump at the rear side of described base, described water tank and water pump are provided with outlet pipe, described outlet pipe is communicated with some described nozzles;
Be provided with on the right side of described base driving second bearing near or away from the first translation assembly of the first bearing;
Described chassis left side be provided with driving three abutments near or away from the second translation assembly of the first bearing.
2. cell chulture test tube according to claim 1 cleans line, it is characterized in that, described first translation assembly comprises the first tooth bar, the first gear and the 3rd motor, described first tooth bar is fixed on the bottom of the second bearing and the first tooth bar is connected with base sliding, described first pinion rotation to be arranged on base and described first gear by the 3rd motor-driven rotation, described first tooth bar coordinates with the first gears meshing.
3. cell chulture test tube according to claim 1 cleans line, it is characterized in that, described second translation assembly comprises the second tooth bar, the second gear and the 4th motor, described second tooth bar is fixed on the bottom of the first bearing and the second tooth bar is connected with base sliding, described second pinion rotation to be arranged on base and described second gear by the 4th motor-driven rotation, described second tooth bar coordinates with the second gears meshing.
4. cell chulture test tube according to claim 1 cleans line, it is characterized in that, described in each, the both sides of movable block are respectively equipped with otic placode, and the test tube dish of described movable block both sides is provided with fixed block, and described spring is arranged between fixed block and corresponding otic placode.
5. cell chulture test tube according to claim 4 cleans line, it is characterized in that, described test tube dish and brush plate are working of plastics.
6. cell chulture test tube according to claim 5 cleans line, it is characterized in that, be provided with circular hole at the center of described test tube dish, described runner is placed in circular hole.
7. cell chulture test tube according to claim 6 cleans line, it is characterized in that, described test tube dish is provided with draw-in groove, and in described draw-in groove, snap-fit engagement has the end cap covered on movable block.
8. cell chulture test tube according to claim 1 cleans line, it is characterized in that, in the semicircle orifice of described groove and movable block, be equipped with rubber blanket.
CN201510413884.8A 2015-07-15 2015-07-15 Test tube cleaning line for cell culture Pending CN104998868A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510413884.8A CN104998868A (en) 2015-07-15 2015-07-15 Test tube cleaning line for cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510413884.8A CN104998868A (en) 2015-07-15 2015-07-15 Test tube cleaning line for cell culture

Publications (1)

Publication Number Publication Date
CN104998868A true CN104998868A (en) 2015-10-28

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107019476A (en) * 2016-11-03 2017-08-08 刘语涵 Domestic wine glass cleaning storage all-in-one
CN110479702A (en) * 2019-07-29 2019-11-22 马鞍山致青工业设计有限公司 A kind of rotary building pipe cleaning device gripped

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2395817Y (en) * 1999-11-26 2000-09-13 马居风 Test tube washing device
US6405738B1 (en) * 1999-10-22 2002-06-18 Ultrafryer Systems, Inc. Spray cleaning apparatus for deep fryer
FR2865144A1 (en) * 2004-01-21 2005-07-22 Tournaire Sa Liquid/solid separator for use in chemistry, pharmacy and agro-food industry has suction unit to remove accumulated powder from filter surface
CN202005380U (en) * 2011-04-29 2011-10-12 弗曼燊生物科技(上海)有限公司 Multi-head test-tube brush
CN202316442U (en) * 2011-11-01 2012-07-11 陈申芝 Device for automatically cleaning test tube
US8734589B1 (en) * 2009-09-11 2014-05-27 The Rectorseal Corporation Use of liquid flushing composition in cleaning a heat exchanger
CN204381028U (en) * 2015-01-06 2015-06-10 宫志美 Medical test test tube cleaning device
CN204842390U (en) * 2015-07-15 2015-12-09 济南大学 Test tube washing line for cell culture

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6405738B1 (en) * 1999-10-22 2002-06-18 Ultrafryer Systems, Inc. Spray cleaning apparatus for deep fryer
CN2395817Y (en) * 1999-11-26 2000-09-13 马居风 Test tube washing device
FR2865144A1 (en) * 2004-01-21 2005-07-22 Tournaire Sa Liquid/solid separator for use in chemistry, pharmacy and agro-food industry has suction unit to remove accumulated powder from filter surface
US8734589B1 (en) * 2009-09-11 2014-05-27 The Rectorseal Corporation Use of liquid flushing composition in cleaning a heat exchanger
CN202005380U (en) * 2011-04-29 2011-10-12 弗曼燊生物科技(上海)有限公司 Multi-head test-tube brush
CN202316442U (en) * 2011-11-01 2012-07-11 陈申芝 Device for automatically cleaning test tube
CN204381028U (en) * 2015-01-06 2015-06-10 宫志美 Medical test test tube cleaning device
CN204842390U (en) * 2015-07-15 2015-12-09 济南大学 Test tube washing line for cell culture

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107019476A (en) * 2016-11-03 2017-08-08 刘语涵 Domestic wine glass cleaning storage all-in-one
CN107019476B (en) * 2016-11-03 2019-02-19 刘语涵 Domestic wine glass cleaning storage all-in-one machine
CN110479702A (en) * 2019-07-29 2019-11-22 马鞍山致青工业设计有限公司 A kind of rotary building pipe cleaning device gripped

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