CN104984091A - Method for preparing medicinal material gastrodia elata - Google Patents
Method for preparing medicinal material gastrodia elata Download PDFInfo
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- CN104984091A CN104984091A CN201510428497.1A CN201510428497A CN104984091A CN 104984091 A CN104984091 A CN 104984091A CN 201510428497 A CN201510428497 A CN 201510428497A CN 104984091 A CN104984091 A CN 104984091A
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Abstract
The invention discloses a method for preparing medicinal material gastrodia elata. The method includes the following steps that 1, tubers of gastrodia elata are taken and washed to remove soil; 2, the tubers, processed in the step 1, of the gastrodia elata are soaked in a NaCl water solution; 3, the tubers, processed in the step 2, of gastrodia elata are cleaned and naturally dried to obtain the medicinal material gastrodia elata. According to the medicinal material gastrodia elata prepared through the method, the medicine yield is 30-38%, measurements of the shape, properties, thin-layer chromatography identification, water content, alcohol extract and gastrodin content all meet quality standards of the astrodia elata item in Pharmacopoeia of the People's Republic of China, generally, the medicinal material gastrodia elata does not fade or is damaged by worms after being placed for one year, and the nitrite content meets related standards. The provided method solves the problem that the tubers of the gastrodia elata rot or mildew in large quantities in a natural drying method, can replace a sulfur fumigation method and a drying method and has high application value.
Description
Technical field
The present invention relates to a kind of method preparing rhizome traditional Chinese medicinal materials, particularly relate to a kind of method preparing medical material Rhizoma Gastrodiae.
Background technology
Medical material Rhizoma Gastrodiae is the dry tuber of orchid Rhizoma Gastrodiae Gastrodia elata Bl., is a kind of conventional rare Chinese medicine.Excavated to Clear and Bright next year after the Beginning of Winter.General Requirements, cleans immediately, steams thoroughly, opens wide cold drying.
The tuber of Rhizoma Gastrodiae is thicker, processing of crude drugs be whole tuber, steam the cold drying needing the long period thoroughly, the time of meeting overcast and rainy then cold drying is longer, and often occurs to rot, and causes medical material to lose in a large number, brings certain economic loss to medicinal herb grower.Especially in the main producing region, southwest, river, China Yunnan-Guizhou that relative humidity is larger.Clear water cooking method or 0.2% vitriol water-boiling method are also the methods adopted in the Rhizoma Gastrodiae course of processing, but in the medical material Rhizoma Gastrodiae of these 2 kinds of method processing, the content of effective ingredient gastrodine is significantly lower than Powder Made by Steamed Method.
By the Rhizoma Gastrodiae that Powder Made by Steamed Method, clear water cooking method obtain, during storing in a warehouse, easily produce phenomenon of damaging by worms.
Sulfur fumigation method is a kind of habitat processing method of Rhizoma Gastrodiae, can obtain Rhizoma Gastrodiae quickly, produces hardly and rots and loss, have good outward appearance and color and luster, and solve the problem that medical material damages by worms.But the method is residual because of sulfur, and likely causing the reduction of drug effect, the generation of toxic and side effects, thus affect the quality of medicinal material of Rhizoma Gastrodiae, is the current technology that should forbid or limit.
Therefore, invent a kind of novel dry technology, the tuber of the large quantities of fresh plant Rhizoma Gastrodiae of results can be made at source to obtain dry fast and effectively, prevent rotting and going mouldy of a large amount of fresh medicinal raw material, sulfur fumigation technology can be substituted again, limit adding and remaining of sulfur, simultaneously, can also not producing the phenomenon of damaging by worms during storing in a warehouse, ensure the quality of medicinal material of Rhizoma Gastrodiae, is a problem needing to solve.
Salting method is mainly raw material with vegetable, mostly uses saline to pickle.GB 5009.33-2010 " mensuration of national food safety standard nitrite in food bath nitrate ", GB 2714-2003 " catsup and pickled vegetables sanitary standard " require that Pickle nitrite is (with NaNO
2meter) content standard be≤20mg/kg, the limit standard of the nitrite of Ministry of Agriculture NY/T 437-2000 " pollution-free food catsup and pickled vegetables " is≤4mg/kg.Simulation vegetable and meat products pickle method, that carries out Chinese crude drug kills enzyme and dried, is conducive to the storage of Chinese crude drug, there is not yet the report of document.
Summary of the invention
The object of this invention is to provide a kind of method preparing medical material Rhizoma Gastrodiae, the method first adopts the tuber of salting method process plant Rhizoma Gastrodiae, then can obtain described medical material Rhizoma Gastrodiae through natural drying; The method can make the moisture in the tuber of plant Rhizoma Gastrodiae reduce fast on the one hand, shortens drying time, can suppress the activity of various microorganism simultaneously, prevent generation that is rotten or iso-metamorphism phenomenon of going mouldy, in addition, can also ensure during storing in a warehouse, do not produce the phenomenon of damaging by worms, there is wide using value.
A kind of method preparing medical material Rhizoma Gastrodiae provided by the invention, comprises the steps:
(1) get the tuber of plant Rhizoma Gastrodiae, wash away earth;
(2) tuber of the plant Rhizoma Gastrodiae that will process through step (1), is soaked in NaCl aqueous solution;
(3) clean the tuber of the plant Rhizoma Gastrodiae processed through step (2), described medical material Rhizoma Gastrodiae after natural drying, can be obtained.
In above-mentioned method, in step (1), described plant Rhizoma Gastrodiae can be wild or pseudo-wild cultivating in Yunnan Province, the plant Rhizoma Gastrodiae in Guizhou Province or Shaanxi Province, specifically can be the wild or plant Rhizoma Gastrodiae of pseudo-wild cultivating in Yiliang County, Yunnan Province, the wild or plant Rhizoma Gastrodiae of pseudo-wild cultivating in Dejiang County, Guizhou Province, wild or pseudo-wild cultivating in the plant Rhizoma Gastrodiae of Chenggu County, Shaanxi Province.
In above-mentioned method, in step (2), utilize high concentration saline solution that the moisture in plant cell is oozed out under the hyperosmosis effect of Sal, thus reach the object reducing moisture in plant fast; Utilize the effect of hyperosmosis, make enzyme molecule solidify degeneration and inactivation; The activity of various microorganism can be suppressed simultaneously, prevent generation that is rotten or iso-metamorphism phenomenon of going mouldy;
Described NaCl aqueous solution is prepared by Sal and water, the mass body volume concentrations of described NaCl aqueous solution can be 3 ~ 10g/100mL, specifically can be 3 ~ 8g/100mL NaCl aqueous solution, 3 ~ 7g/100mL NaCl aqueous solution, 3 ~ 5g/100mL NaCl aqueous solution, 5 ~ 10g/100mL NaCl aqueous solution, 5 ~ 8g/100mL NaCl aqueous solution, 5 ~ 7g/100mL NaCl aqueous solution, 7 ~ 10g/100mL NaCl aqueous solution, 7 ~ 8g/100mL NaCl aqueous solution or 8 ~ 10g/100mL NaCl aqueous solution.
In above-mentioned method, in step (2), described immersion can be carried out in hermetic container, and described container is the container that can be used for arbitrarily pickling, and includes but not limited to sauerkraut cylinder, jar for picking vegetable, cement pit etc.
In above-mentioned method, in step (2), during described immersion, the addition of described NaCl aqueous solution ensures the tuber not having described plant Rhizoma Gastrodiae.
In above-mentioned method, in step (2), the time effects dehydration of described immersion and the effect of suppression microorganism, also can affect the drug rate of plant Rhizoma Gastrodiae simultaneously; The time of described immersion can be 6 ~ 15 days, specifically can be 6 ~ 8 days, 6 ~ 12 days, 6 ~ 15 days, 8 ~ 12 days, 8 ~ 15 days or 12 ~ 15 days.
In above-mentioned method, in step (2), the generation of mildew phenomena in container, described immersion comprises the steps: 1) soak 1 ~ 2 day in described NaCl aqueous solution, specifically can soak 1 day or 2 days; 2) change described NaCl aqueous solution, continue immersion 5 ~ 14 days, specifically can soak 6 ~ 14 days, 6 ~ 10 days, 10 ~ 14 days, 6 days, 10 days or 14 days.Concrete operations can be as follows: 1) soaked 1 ~ 2 day in the container that described NaCl aqueous solution is housed by the tuber of plant Rhizoma Gastrodiae; 2) the NaCl aqueous solution in container described in reject, adds the described NaCl aqueous solution of same concentrations again, is soaked 5 ~ 14 days by the tuber of plant Rhizoma Gastrodiae.
In above-mentioned method, in step (3), described cleaning can remove salt unnecessary in described plant Gastrodia Elata Stem Tuber, circulating water specifically can be adopted to rinse and/or be soaked in clear water and clean, after natural drying, can obtain described plant Rhizoma Gastrodiae.
The present invention has following beneficial effect:
The tuber of salting method process plant Rhizoma Gastrodiae is adopted in the present invention, utilize high concentration Sal that the moisture in plant cell is oozed out under the hyperosmosis effect of Sal, what be not only conducive to wherein enzyme composition solidifies degeneration and inactivation, and the object reducing moisture in plant fast can be reached, suppress the activity of various microorganism, prevent to rot or iso-metamorphism phenomenon of going mouldy.Adopt medical material Rhizoma Gastrodiae prepared by method provided by the invention, drug rate is 30-38%, the mensuration of character, indentification by TLC, moisture, ethanol soluble extraction, gastrodin content all reaches the quality standard under Pharmacopoeia of the People's Republic of China Rhizoma Gastrodiae item, place under usual conditions within 1 year, have no fade, phenomenon of damaging by worms, and content of nitrite meets relevant criterion.The problem that the tuber that method provided by the invention solves plant Rhizoma Gastrodiae in natural seasoning rots in a large number or goes mouldy, can substitute sulfur fumigation and steaming method and oven drying method, have wide using value.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.
Experimental technique in following embodiment, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is market and can purchases available.Gastrodine purchased from Beijing Century AudioCodes Bioisystech Co., Ltd, lot number 140518, purity >=98% (HPLC).Dry standard: weigh in interval for 24 hours, weight does not change.Pickle jar, Pickles jar, pressure stone, scald with boiled water before use.Cement pit (Pickles pond), cleans one time with water before use, accomplishes without dirt, no-sundries.Rhizoma Gastrodiae control medicinal material examines and determine institute, for the tuber of plant Rhizoma Gastrodiae grinds the powder obtained purchased from Chinese pharmaceutical biological product.
Indentification by TLC: replace and survey sample powder 0.5g, add 70% methanol 5mL, supersound process 30 minutes, filters, gets filtrate as need testing solution.Separately get Rhizoma Gastrodiae control medicinal material 0.5g, be made in the same way of control medicinal material solution.Get citrulline reference substance again, add methanol and make the solution of every 1mL containing 1mg, product solution in contrast.Test according to the Pharmacopoeia of the People's Republic of China (2010 editions) annex VI B thin layer chromatography, draw need testing solution 10 μ L, control medicinal material solution and reference substance solution 5 μ L, put respectively on same silica gel g thin-layer plate, with acetate-methanol-water (9:1:0.2) for developing solvent, launch, take out, dry, spray, with 10% phosphomolybdic acid ethanol solution, is heated to spot development at 105 DEG C clear.Meet the requirement " in test sample chromatograph, on the position corresponding with reference substance chromatograph to control medicinal material chromatograph, the speckle of aobvious same color " under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item.
Ethanol soluble extraction measures: according to the alcohol hot dipping under the Pharmacopoeia of the People's Republic of China (2010 editions) annex XA item, use 95% ethanol as solvent, measures the amount of extractum.The measured value meeting extractum under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item requires " must not be less than 10.0% ".
The assay of gastrodine: carry out the mensuration of gastrodin content according to the assay method of high performance liquid chromatography under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item.Get Rhizoma Gastrodiae powder (crossing No. three sieves) about 2g, accurately weighed, put in tool plug conical flask, precision adds 75% ethanol 50mL, weighed weight, reflux 3 hours, lets cool, more weighed weight, the weight of less loss is supplied with 75% ethanol, shake up, filter, precision measures subsequent filtrate 10mL, be concentrated near dry, residue adds acetonitrile-water (3:97) mixed solution and dissolves, and is transferred in 25mL measuring bottle, is diluted to scale with acetonitrile-water (3:97) mixed solution, shake up, filter, get subsequent filtrate, obtain need testing solution.Get gastrodine reference substance appropriate, accurately weighed, add acetonitrile-0.05% phosphoric acid solution (3:97) and make the solution of every 1mL containing 50 μ g, obtain reference substance solution.Adopt Shimadzu LC-20A high performance liquid chromatograph, take octadecylsilane chemically bonded silica as filler, with acetonitrile-0.05% phosphoric acid solution (3:97) for mobile phase, determined wavelength is 220nm, and number of theoretical plate calculates by gastrodine peak and is not less than 5000; Accurate absorption reference substance solution and each 10 μ L of need testing solution respectively, injection liquid chromatography, measures, and calculates, obtains the content of Gastrodin in Gastrodia eleta Bl..Require that this product calculates by dry product, containing gastrodine (C under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item
13h
18o
7) amount be " must not 0.20% be less than ".
Nitrite Limited Doses measures: the assay method according to GB 5009.33-2010 " mensuration of the national food safety standard nitrite in food bath nitrate " chromatography of ions carries out the determination limit of nitrite.Take Rhizoma Gastrodiae sample powder (crossing No. three sieves) 5.0g, wash in 100mL volumetric flask with ultra-pure water, concussion shakes up and is settled to 100mL, supersound extraction 30min (shakes up 1 time every 5min concussion, solid phase is kept to disperse completely), with the centrifugal 10min of 10000r/min, get supernatant; Supernatant (is passed through with methanol 10mL, water 15mL before use successively by silent your the OnGuard II RP post of generation that flies of U.S.'s match successively, leave standstill activation 30min), (water in advance 10mL passes through OnGuard II Ag post, leave standstill activation 30min), (water in advance 10mL passes through OnGuard II Na post, leave standstill activation 30min), collect continuous eluent after discarding the first eluent of 3mL, be need testing solution.(standard value is the food inspection sodium nitrite standard solution of 200 μ g/mL to get sodium nitrite standard solution 5.00mL, GBW (E) 100156, Beijing Haian Hongmeng Standard Substance Technology Co., Ltd.), be settled in 100mL volumetric flask, be mixed with the standard solution of 10.0mg/L.Adopt the U.S. to wear peace ICS-2000 ion chromatograph, be equipped with the online generating means of leacheate, DS6 electric conductivity detector, ASRS300-4 type suppressor, 50 μ L quantitative loop.Chromatographic column is Dionex IonPac AS19 (250mm × 4mm); guard column is Dionex IonPac AG19 (50mm × 4mm); column temperature 30 DEG C; leacheate is KOH solution (concentration is 6-70mmol/L); gradient 6mmol/L rises to 70mmol/L after keeping 30min in 0.1min, keeps 6min, then be down to 6mmol/L in 0.1min; keep 6min, flow velocity 1.0mL/min.Detector is electric conductivity detector, and detection cell temperature is 35 DEG C.Sampling volume is 50 μ L.The reagent related to is: methanol (chromatographically pure), ultra-pure water (resistivity is 18.2 Ω cm, prepared by Millipore ultrapure water machine).Thought by methodological research, detection limit (LOD, S/N=3) and the quantitative limit (LOQ, S/N=10) of the method are respectively 3.3mg/kg and 10.0mg/kg.
In following embodiment, the computing formula of drug rate is as follows: the quality ÷ of drug rate=medical material Rhizoma Gastrodiae is for the preparation of quality × 100% of the tuber of the plant Rhizoma Gastrodiae of medical material Rhizoma Gastrodiae.
Adopt China Salt Industry Co. to supervise in following embodiment, Shandong Han Ting first saltern produce Sal (NaCl content is denoted as and is more than or equal to 91.0%) prepare NaCl aqueous solution.The preparation method of NaCl aqueous solution: after water is boiled, cool to room temperature, adds the Sal not waiting weight portion in water, stir and make it dissolve completely, now with the current.The concentration of NaCl aqueous solution refers to the concentration of NaCl in water.
Embodiment 1, prepare medical material Rhizoma Gastrodiae with the tuber of planting in the plant Rhizoma Gastrodiae of Yiliang County, Yunnan Province
One, the salting legal system in pickle jar is for medical material Rhizoma Gastrodiae (carrying out in Beijing)
1, late January, excavate the tuber of plant Rhizoma Gastrodiae from Yiliang County, Yunnan Province, after being delivered to Beijing soon, wash away earth.
2, select without disease and pest, the complete tuber of variable color of not rotting, do not have, get 1.2kg, pile up in pickle jar, push down with pressure stone, add 3g/100mL NaCl aqueous solution, the dipped tuber of the water surface, lid covers, and pickles 6 days.
3, take out tuber, wash 1 hour with the clear water of flowing, the tuber of Rhizoma Gastrodiae is soft shape, then natural drying, obtains medical material Rhizoma Gastrodiae.
Carry out 3 batches of reprocessings, all do not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 33.3%.
Two, the salting legal system in cement pit is for medical material Rhizoma Gastrodiae (carrying out in Yiliang County, Yunnan Province)
1, late January, excavate the tuber of plant Rhizoma Gastrodiae from Yiliang County, Yunnan Province, wash away earth.
2, select without disease and pest, the complete tuber that do not rot, do not have variable color, get 15kg, be deposited in cement pit, push down with pressure stone, add 10g/100mL NaCl aqueous solution, the dipped tuber of the water surface, seals face, pond with plastic foil, pickles 2 days; Extract the saline in cement pit out, add new 10g/100mL NaCl aqueous solution, the dipped tuber of the water surface, face, pond covers with plastic foil, pickles 10 days.
3, after completing steps 2, rinse and soak 3 hours with clear water, the tuber of Rhizoma Gastrodiae is soft shape, then natural drying, obtains medical material Rhizoma Gastrodiae.
Carry out 3 batches of reprocessings, all do not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 35.8%.
Three, directly dry and prepare medical material Rhizoma Gastrodiae (carrying out in Beijing and Yiliang County, Yunnan Province)
1, with 1 of step one.
2, after completing steps 1, select without disease and pest, the complete tuber that do not rot, do not have variable color, get 1.0kg, natural drying.
2 batches of reprocessings have all been carried out in various places, all there occurs surface stain, numb body rots or mouldy iso-metamorphism phenomenon, could not process medical material Rhizoma Gastrodiae.
Four, evaluate
1, character: the character of each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations is: in ellipse, slightly time, shrinkage and slightly bending, long 5-8cm, wide 2-3cm, thick 1-2cm; Surface light yellowish brown, has vertical wrinkle and is taken turns by the horizontal ring grain of resting bud arrangement more; There is rufous on top to the bud of light brown parrot mouth shape or residual stem foot, and another end has round umbilicus shape cicatrix; Matter is hard, not frangibility, and section is smooth, yellow-white, cutin sample; Feeble QI, sweet in the mouth.Meet the character requirement under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item.
2, thin layer chromatography: each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations, on the position corresponding with reference substance chromatograph to control medicinal material chromatograph, the fluorescence speckle of all aobvious same color.Meet the requirement of indentification by TLC under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item.
3, determination of extractives: the average ethanol hot dipping apparent extract of each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations is respectively 10.7%, 10.4%, all meets the requirement that extractum under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item must not be less than 10.0%.
4, determination of moisture: after step one, the two each 3 batches of medical material Rhizoma Gastrodiaes prepared place nearly a year in Pekinese's laboratory, the average moisture content measuring each 3 batches of medical material Rhizoma Gastrodiaes is respectively 10.8%, 9.5%, all meets the requirement must not crossing 15.0% under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item under moisture check item.
5, the assay of gastrodine: the content of average gastrodine of each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations is 0.98%, 0.82%, all meets the amount of gastrodine under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item and must not be less than the requirement of 0.20%.
6, nitrite determination limit: the NaNO of each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations
2content, all lower than the detectability of 3.3mg/kg, all meets the nitrite limit standard of GB 2714-2003 " catsup and pickled vegetables sanitary standard " and Ministry of Agriculture NY/T437-2000 " pollution-free food catsup and pickled vegetables ".
7, the Rhizoma Gastrodiaes of step one, two preparations, at Pekinese's laboratory, place nearly one-year age respectively, all do not occur variable color, phenomenon of damaging by worms.
Embodiment 2, prepare medical material Rhizoma Gastrodiae with the tuber of planting in the plant Rhizoma Gastrodiae of Dejiang County, Guizhou Province
One, the salting legal system in Pickles jar is for medical material Rhizoma Gastrodiae (carrying out in Beijing)
1, late Febuary, excavate the tuber of plant Rhizoma Gastrodiae from Dejiang County, Guizhou Province, after being delivered to Beijing soon, wash away earth.
2, select without disease and pest, the complete tuber that do not rot, do not have variable color, get 5.8kg, pile up in Pickles jar, push down with pressure stone, add 5g/100mL NaCl aqueous solution, the dipped tuber of the water surface, lid covers, and pickles 1 day; Release the saline in Pickles jar, add new 5g/100mL NaCl aqueous solution, the dipped tuber of the water surface, lid lid, pickles 14 days.
3, rinse with clear water and soak 5 hours, the tuber of Rhizoma Gastrodiae is soft shape, then natural drying, obtains medical material Rhizoma Gastrodiae.
Carry out 3 batches of reprocessings, all do not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 30.0%.
Two, directly dry and prepare medical material Rhizoma Gastrodiae (carrying out in Beijing)
1, with 1 of step one.
2, after completing steps 1, select without disease and pest, the complete tuber that do not rot, do not have variable color, get 1.0kg, natural drying.
Carry out 2 batches of reprocessings, all there occurs surface stain, numb body has rotted or mouldy iso-metamorphism phenomenon, medical material Rhizoma Gastrodiae could not have been processed.
Three, evaluate
1, character: the character of 3 batches of medical material Rhizoma Gastrodiaes prepared by step one is: in ellipse, slightly time, shrinkage and slightly bending, long 7-12cm, wide 3-4cm, thick 1-2cm; Surface light yellowish brown, has vertical wrinkle and is taken turns by the horizontal ring grain of resting bud arrangement more; There is rufous on top to the bud of light brown parrot mouth shape or residual stem foot, and another end has round umbilicus shape cicatrix; Matter is hard, not frangibility, and section is smooth, yellow-white to light brown, cutin sample; Feeble QI, sweet in the mouth.Meet the character requirement under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item.
2, thin layer chromatography: 3 batches of medical material Rhizoma Gastrodiaes prepared by step one, on the position corresponding with reference substance chromatograph to control medicinal material chromatograph, the fluorescence speckle of all aobvious same color.Meet the requirement of indentification by TLC under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item.
3, determination of extractives: the average ethanol hot dipping apparent extract of 3 batches of medical material Rhizoma Gastrodiaes prepared by step one is respectively 12.2%, meets the requirement that extractum under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item must not be less than 10.0%.
4, determination of moisture: after 3 batches of medical material Rhizoma Gastrodiaes prepared by step one place nearly a year in Pekinese's laboratory, the average moisture content measuring each 3 batches of medical material Rhizoma Gastrodiaes is respectively 11.3%, meets the requirement must not crossing 15.0% under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item under moisture check item.
5, the assay of gastrodine: the content of average gastrodine of 3 batches of medical material Rhizoma Gastrodiaes prepared by step one is 0.42%, the amount of gastrodine under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item that meets must not be less than the requirement of 0.20%.
6, nitrite determination limit: the NaNO of 3 batches of medical material Rhizoma Gastrodiaes prepared by step one
2content, all lower than the detectability of 3.3mg/kg, all meets the nitrite limit standard of GB 2714-2003 " catsup and pickled vegetables sanitary standard " and Ministry of Agriculture NY/T437-2000 " pollution-free food catsup and pickled vegetables ".
7, the Rhizoma Gastrodiae prepared of step one, at Pekinese's laboratory, places nearly 1 year, does not occur variable color, phenomenon of damaging by worms.
Embodiment 3, prepare medical material Rhizoma Gastrodiae with the tuber of planting in the plant Rhizoma Gastrodiae of Chenggu County, Shaanxi Province
One, the salting legal system in Pickles jar is for medical material Rhizoma Gastrodiae (carrying out in Beijing)
1, mid-March, excavate the tuber of plant Rhizoma Gastrodiae from Chenggu County, Shaanxi Province, after being delivered to Beijing soon, wash away earth.
2, select without disease and pest, the complete tuber of variable color of not rotting, do not have, get 5.0kg, pile up in Pickles jar, push down with pressure stone, add 7g/100mL NaCl aqueous solution, the dipped tuber of the water surface, lid covers, and pickles 8 days.
3, take out tuber, wash 2 hours with the clear water of flowing, the tuber of Rhizoma Gastrodiae is soft shape, then natural drying, obtains medical material Rhizoma Gastrodiae.
Carry out 3 batches of reprocessings, all do not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 37.0%.
Two, the salting legal system in cement pit is for medical material Rhizoma Gastrodiae (carrying out in Chenggu County, Shaanxi Province)
1, late January, excavate the tuber of plant Rhizoma Gastrodiae from Chenggu County, Shaanxi Province, wash away earth.
2, select without disease and pest, the complete tuber that do not rot, do not have variable color, get 10kg, be deposited in cement pit, push down with pressure stone, add 8g/100mL NaCl aqueous solution, the dipped tuber of the water surface, seals face, pond with plastic foil, pickles 2 days; Extract the saline in cement pit out, add new 8g/100mL NaCl aqueous solution, the dipped tuber of the water surface, face, pond covers with plastic foil, pickles 6 days.
3, rinse with clear water and soak 4 hours, the tuber of Rhizoma Gastrodiae is soft shape, then natural drying, obtains medical material Rhizoma Gastrodiae.
Carry out 3 batches of reprocessings, all do not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 37.9%.
Three, directly dry and prepare medical material Rhizoma Gastrodiae (carrying out in Beijing and Chenggu County, Shaanxi Province)
1, with 1 of step one.
2, after completing steps 1, select without disease and pest, the complete tuber that do not rot, do not have variable color, get 1.0kg, natural drying.
2 batches of reprocessings have all been carried out in various places, all there occurs surface stain, numb body rots or mouldy iso-metamorphism phenomenon, could not process medical material Rhizoma Gastrodiae.
Four, evaluate
1, character: the character of each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations is: in ellipse, slightly time, shrinkage and slightly bending, long 9-13cm, wide 4-6cm, thick 1-2cm; Surface light yellowish brown, has vertical wrinkle and is taken turns by the horizontal ring grain of resting bud arrangement more; There is rufous on top to the bud of light brown parrot mouth shape or residual stem foot, and another end has round umbilicus shape cicatrix; Matter is hard, not frangibility, and section is smooth, yellow-white to light brown, cutin sample; Feeble QI, sweet in the mouth.Meet the character requirement under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item.
2, thin layer chromatography: each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations, on the position corresponding with reference substance chromatograph to control medicinal material chromatograph, the fluorescence speckle of all aobvious same color.Meet the requirement of indentification by TLC under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item.
3, determination of extractives: the average ethanol hot dipping apparent extract of each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations is respectively 13.2%, 13.4%, all meets the requirement that extractum under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item must not be less than 10.0%.
4, determination of moisture: after step one, the two each 3 batches of medical material Rhizoma Gastrodiaes prepared place nearly a year in Pekinese's laboratory, the average moisture content measuring each 3 batches of medical material Rhizoma Gastrodiaes is respectively 12.3%, 9.4%, all meets the requirement must not crossing 15.0% under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item under moisture check item.
5, the assay of gastrodine: the content of average gastrodine of each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations is 0.33%, 0.25%, all meets the amount of gastrodine under the Pharmacopoeia of the People's Republic of China (2010 editions) Rhizoma Gastrodiae item and must not be less than the requirement of 0.20%.
6, nitrite determination limit: the NaNO of each 3 batches of medical material Rhizoma Gastrodiaes of step one, two preparations
2content, all lower than the detectability of 3.3mg/kg, all meets the nitrite limit standard of GB 2714-2003 " catsup and pickled vegetables sanitary standard " and Ministry of Agriculture NY/T437-2000 " pollution-free food catsup and pickled vegetables ".
7, the Rhizoma Gastrodiaes of step one, two preparations, at Pekinese's laboratory, place nearly one-year age respectively, all do not occur variable color, phenomenon of damaging by worms.
Claims (4)
1. prepare a method for medical material Rhizoma Gastrodiae, comprise the steps:
(1) get the tuber of plant Rhizoma Gastrodiae, wash away earth;
(2) tuber of the plant Rhizoma Gastrodiae that will process through step (1), is soaked in NaCl aqueous solution;
(3) clean the tuber of the plant Rhizoma Gastrodiae processed through step (2), described medical material Rhizoma Gastrodiae after natural drying, can be obtained.
2. method according to claim 1, is characterized in that: in step (2), and in described NaCl aqueous solution, the mass body volume concentrations of NaCl is 3 ~ 10g/100mL.
3. method according to claim 1 and 2, is characterized in that: in step (2), and the time of described immersion is 6 ~ 15 days.
4. method according to claim 3, is characterized in that: in step (2), described immersion comprises the steps: 1) soak 1 ~ 2 day in described NaCl aqueous solution; 2) change described NaCl aqueous solution, continue immersion 5 ~ 14 days.
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| CN107821901A (en) * | 2017-11-23 | 2018-03-23 | 何会平 | A kind of brain health gastrodia tuber solid beverage |
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| CN103877399A (en) * | 2014-03-26 | 2014-06-25 | 贵州乌蒙腾菌业有限公司 | Pure natural fresh gastrodia elata processing method |
| CN104306747A (en) * | 2014-11-07 | 2015-01-28 | 南京中医药大学 | Rhizome gastrodiae processing method |
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| CN103877399A (en) * | 2014-03-26 | 2014-06-25 | 贵州乌蒙腾菌业有限公司 | Pure natural fresh gastrodia elata processing method |
| CN104306747A (en) * | 2014-11-07 | 2015-01-28 | 南京中医药大学 | Rhizome gastrodiae processing method |
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