CN104971095B - A kind of Chinese medicinal compound extract and preparation method thereof with antidepressant activity - Google Patents
A kind of Chinese medicinal compound extract and preparation method thereof with antidepressant activity Download PDFInfo
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Abstract
The present invention relates to one with antidepressant activity Chinese medicinal compound extract and preparation method thereof, be by Radix Glycyrrhizae, rhizoma menispermi, ramulus cinnamomi and Rhizoma Chuanxiong be raw material, weight proportion are as follows: 2-5 parts of Radix Glycyrrhizae, 5-8 parts of rhizoma menispermi, 8-10 parts of ramulus cinnamomi, 1-4 parts of Rhizoma Chuanxiong.Compatibility science of the present invention, preparation process is reasonable, the effective component of each Chinese medicine material is not destroyed, it has no toxic side effect, through experiments, it was found that the extract can substantially reduce dead time and significant antagonism reserpine and the caused mouse temperature decline effect that behavioral despair model mice hangs tail and forced swimming in the case where not influencing autonomic activities, therefore it has remarkable result to anti-depression aspect is supported, and four taste Chinese medicines have good synergistic effect, have good market prospects.
Description
Technical field
The present invention relates to a kind of Chinese medicinal compound extract and preparation method thereof with antidepressant activity, belongs to Chinese medicine
Extracting method and technical field of traditional Chinese medicine preparation.
Technical background
It with depressive mood is the disease mainly showed that depression, which is a kind of, due to cannot cause directly to endanger to body usually
It is ignored.Currently, being 15 kinds of main one of reasons that disable in depression in developed country.According to the data in the U.S., the U.S. is every
Year, there are about 11,000,000 people to suffer from clinical depression, and the homicide rate of clinical depression patient is 10 ~ 15%, is influenced by this disease, annual big
About lose 20,000,000,000 dollars.National survey data shows that the homicide rate of depression is about 20 times higher than general population.It can be seen that depression
It is very big to the threat of human health.The year two thousand twenty is predicted, depression is only second to the second largest disability-causing factor of the mankind of cancer by becoming.
Research data shows typical psychosomatic disease recently for psychosomatic medicine, medical psychology, psychiatry etc. --- the existing trouble of depression
Sick rate has been more than cardiovascular and cerebrovascular disease and tumour, first for leaping to developed country.
The Western medicine for treating all kinds of depression at present mainly has tricyclic antidepressants (abbreviation TCA is divided into Iminodibenzyl, Pu Lin
Class, dibenzo table class etc.), monoamine oxidase inhibitors (abbreviation MAOI, such as Iproniazid) and 5-HT reuptaking inhibitor (referred to as
SSRI, such as Prozac), but the drawbacks such as the generally existing drug resistance of Western medicine, toxic side effect be big, therefore find safe and efficient, secondary
Act on that small antidepressant is particularly important, especially natural antidepressant becomes the research hotspot in the field.
Summary of the invention
The present invention discloses a kind of Chinese medicinal compound extract with antidepressant activity, there is apparent medical treatment to make depression
With.
Invention also provides the preparation processes of the compound Chinese medicinal preparation extract, are convenient for industrialized production.
A kind of Chinese medicinal compound extract with antidepressant activity of the present invention, it is characterised in that be by following raw material
Made of by ratio of weight and the number of copies:
Radix Glycyrrhizae 2-5, rhizoma menispermi 5-8, ramulus cinnamomi 8-10, Rhizoma Chuanxiong 1-4.
A kind of Chinese medicinal compound extract with antidepressant activity of the present invention, it is characterised in that optimum ratio are as follows:
Radix Glycyrrhizae 4, rhizoma menispermi 7, ramulus cinnamomi 9, Rhizoma Chuanxiong 3.
The preparation method of the compound Chinese medicinal preparation of antidepressant activity of the present invention, comprising the following steps:
1, Radix Glycyrrhizae, ramulus cinnamomi and Rhizoma Chuanxiong are proportionally deployed, and are placed in the 70-80% alcohol reflux measured in container with 10-15 times and are mentioned
It takes 3 times, 1-2 hours each, filtering and recycle ethanol extract and being concentrated into room temperature relative density is 1.15-1.20, spraying dry
It is dry to get alcohol extracting thing；
2, rhizoma menispermi decocts 3 times after being impregnated 3-5 hours with 5-10 times of amount, 1-2 hours each, filters and recycle-water extracts
Liquid, being concentrated into room temperature relative density is 1.05-1.15, is spray-dried to get water extract；
It smashs to pieces and to be dried, crushed into fine powder spare；
3, alcohol extracting thing and water extract are uniformly mixed to get traditional Chinese medicine extraction dry powder.
The compound Chinese medicinal preparation of antidepressant activity of the present invention answering in the mental diseases drugs such as treatment depression
With.
Compound preparation of the present invention belongs to pure Chinese medicine oral preparations, have antidepressant effect, can with it is one or more pharmaceutically
Acceptable carrier (auxiliary material) is configured to medicament, being capable of being applied in treatment depressive illness drug well.
The beneficial effects of the present invention are: cooperating Radix Glycyrrhizae Rhizoma Chuanxiong to form compound, selected drug using rhizoma menispermi, ramulus cinnamomi as main ingredient
It is natural medicinal plants, compatibility science between each medicine has apparent synergistic effect, and preparation process is reasonable, does not destroy Chinese medicine
The effective component of raw material, has no toxic side effect, and without additive, has no drug resistance, and to various pain, especially antidepression, effect is obvious,
It can be made into and be suitble to be easy to use using dosage form.
Specific embodiment:
To facilitate the understanding of the present invention, especially exemplified by following embodiment.Its act on be understood to be to explaination of the invention and
It is non-to any type of limitation of the invention.
Embodiment 1
1,20g Radix Glycyrrhizae, ramulus cinnamomi 80g, Rhizoma Chuanxiong 10g are taken, is placed in container with the 80% alcohol reflux extraction of 10 times of amounts 3 times, often
Secondary 1 hour, filtering and recycle ethanol extract and being concentrated into room temperature relative density was 1.15, was spray-dried to get alcohol extracting thing；
2, rhizoma menispermi 50g is taken, is decocted 3 times after being impregnated 3 hours with 5 times of amounts, 1 hour every time, filters and recycle aqueous extract,
Being concentrated into room temperature relative density is 1.10, is spray-dried to get water extract；
3, alcohol extracting thing and water extract are uniformly mixed to get traditional Chinese medicine extraction dry powder.
Embodiment 2
1, extracting liquorice 50g, ramulus cinnamomi 100g, Rhizoma Chuanxiong 40g are placed in 70% alcohol reflux measured in container with 15 times and extract 3 times,
2 hours every time, filtering and recycle ethanol extract and being concentrated into room temperature relative density was 1.20, was spray-dried to get alcohol extracting
Object；
2, rhizoma menispermi 80g is taken, is decocted after being impregnated 5 hours with 10 times of amounts 3 times, 2 hours every time, is filtered and recycle-water extracts
Liquid, being concentrated into room temperature relative density is 1.15, is spray-dried to get water extract；
3, alcohol extracting thing and water extract are uniformly mixed to get traditional Chinese medicine extraction dry powder.
Embodiment 3
1,40g Radix Glycyrrhizae, ramulus cinnamomi 90g, Rhizoma Chuanxiong 30g are taken, is placed in container with the 80% alcohol reflux extraction of 12 times of amounts 3 times, often
Secondary 1.5 hours, filtering and recycle ethanol extract and being concentrated into room temperature relative density was 1.20, was spray-dried to get alcohol extracting
Object；
2, rhizoma menispermi 70g is taken, is decocted after being impregnated 4 hours with 8 times of amounts 3 times, 1.5 hours every time, is filtered and recycle-water extracts
Liquid, being concentrated into room temperature relative density is 1.15, is spray-dried to get water extract；
3, alcohol extracting thing and water extract are uniformly mixed to get traditional Chinese medicine extraction dry powder.
The therapeutic effect of drug of the present invention is further demonstrated that by the experiment of following Typical animal:
According to the Chinese medicinal compound extract that the method provided in 1 ~ embodiment of embodiment 3 is prepared, pharmacodynamics has been carried out
Experiment, result of study are as follows:
1. influence of the anti-depression Chinese medicament compound extract of the present invention to mouse behavioral despair model mice Depressive behavior
50 kunming mices, weight 18-22g are taken, half male and half female is randomly divided into 5 groups, every group 10, is respectively set as sky
White control group, positive controls and anti-anxiety compound extract group of the present invention (1 embodiment of embodiment, 2 embodiment 3), in antidepression
Medicine compound extract each group carries out stomach-filling with the horizontal of 0.2ml/10g, and dosage is respectively 1,2 and 5g/kg, blank control group with
The physiological saline stomach-filling of 0.2ml/10g, positive controls carry out the stomach-filling of 10mg/kg dosage with the horizontal of 0.2ml/10g, and daily one
It is secondary, it continuous 7 days, is put in spacious field case after mouse last dose 1h.Every animal is observed in 5 min, is recorded 3 after each group mouse
Horizontal score (passing through grid number) and vertical score in min (mouse forelimb praises number upwards)；It, will after 1 h of last dose
Animal is put into respectively in 23-25 DEG C of bucket of water temperature, prevents animal hind leg from leaning on bucket bottom to support body.The experiment is by two parts
Composition: 15 min are first carried out and are swum in advance, the swimming behavior in 6 min, the dead time after record in 5 min are observed after 24 h；End
After 1 h of secondary administration, away from the one end for being affixed on filament at about 1 cm of Mouse Tail-tip position with medical proof fabric, the other end and the tension being connected with
Energy converter is connected, about 5 cm of mouse head destage face, once hangs 2 mouse, and centre is separated with blank sheet of paper, and the suspension time is 6
Min, after statistics in 5 min mouse the accumulative dead time.
1) influence of the anti-depression Chinese medicament compound extract to mouse autonomic activities ability
The results are shown in Table 1, after successive administration 7 days, 3 groups of 1 ~ embodiment of anti-depression Chinese medicament compound extract embodiment and fluorine
Western spit of fland control group, in spacious field experiment, spontaneous activity in mice there are no significant compared with blank group the change of divergence.Show antidepression
Chinese medicinal compound extract will not influence the autonomic activities ability of mouse.
Influence (n=10, x ± SEM) of 1 present invention of table to mouse spontaneous activity
| Group | Horizontal score | Vertical score |
| Blank group | 104.1±7.9 | 21.7±1.9 |
| Control group | 112.1±6.7 | 24.4±1.9 |
| Embodiment 1 | 108.4±7.1 | 23.1±2.1 |
| Embodiment 2 | 110.6±7.4 | 20.4±1.7 |
| Embodiment 3 | 117.2±8.7 | 24.2±1.9 |
2) mouse forced swimming test result
The results are shown in Table 2, after successive administration 7 days, 3 groups of 1 ~ embodiment of anti-depression Chinese medicament compound extract embodiment and fluorine
Western spit of fland positive controls are compared to the blank group compared with can substantially reduce dead time of the mouse in forced swim test.
Influence (n=10, x ± SEM) of 2 present invention of table to the mouse forced swimming test dead time
| Group | Dead time/s |
| Blank group | 107.1±12.3 |
| Control group | 79.1±11.2** |
| Embodiment 1 | 104.1±12.3 |
| Embodiment 2 | 81.9±9.1* |
| Embodiment 3 | 81.2±8.1** |
Note: compared with blank group, p < 0.01 * p < 0.05, * *.
The motionless experimental result of 2.3 mouse tail suspensions
The results are shown in Table 3, after successive administration 7 days, 3 groups of 1 ~ embodiment of anti-depression Chinese medicament compound extract embodiment and fluorine
Western spit of fland positive controls are compared to the blank group compared with can substantially reduce dead time of the mouse in forced swim test.
Influence (n=10, x ± SEM) of 3 present invention of table to the mouse tail suspension dead time
| Group | Dead time/s |
| Blank group | 120.1±7.9 |
| Control group | 80.2±7.1** |
| Embodiment 1 | 114.1±8.8 |
| Embodiment 2 | 101.2±9.1* |
| Embodiment 3 | 89.7±7.9** |
Note: compared with blank group, p < 0.01 * p < 0.05, * *.
This experimental study proves that anti-depression Chinese medicament compound extract of the present invention can substantially reduce after successive administration 7 days
Dead time in mouse forced swimming test and tail-suspention test shows certain antidepression ability.
2. the influence of the drug-induced antidepression model mice behavior of anti-depression Chinese medicament compound extract
60 kunming mices, weight 18-22g are taken, half male and half female is randomly divided into 6 groups, every group 10, is respectively set as sky
White control group, positive controls, negative control group and 3 groups of 1 ~ embodiment of anti-anxiety compound extract embodiment, anti-depression Chinese medicament
Compound extract each group carries out stomach-filling with the horizontal of 0.2ml/10g, and dosage is respectively 1,2 and 5g/kg, blank control group with
The physiological saline stomach-filling of 0.2ml/10g, positive controls carry out the stomach-filling of 10mg/kg dosage with the horizontal of 0.2ml/10g, and daily one
It is secondary, continuous 7 days, negative control group with the physiological saline stomach-filling of 0.2ml/10g, after 1 h of last dose respectively to positive controls,
3 groups of 4 mg*kg of intraperitoneal injection of negative control group and 1 ~ embodiment of anti-anxiety compound extract embodiment-1Reserpine carries out respectively
The physiological saline of the observation of temperature decline, the capacity such as blank group intraperitoneal injection measures each group Mice Body after intraperitoneal injection 1 hour
Temperature, after measurement, broken end takes the inferior colliculus cerebral tissue of every animal immediately, and measures dopamine D A and 5- hydroxyl color ammonia in the tissue
The level of sour 5-HT.
1) influence that anti-depression Chinese medicament compound extract tests reserpine induction
It the results are shown in Table described in 4, compared to the blank group compared with positive control drug fluoxetine dose group and anti-depression Chinese medicament compound mention
3 groups of 1 ~ embodiment of object embodiment is taken, the effect of significant antagonism reserpine induction mouse temperature decline is capable of.
The influence (n=10, x ± SEM) that 4 anti-depression Chinese medicament compound extract of table tests reserpine induction
| Group | Body temperature/DEG C |
| Blank group | 37.2±0.6 |
| Model group | 31.9±0.5## |
| Control group | 36.4±1.1** |
| Embodiment 1 | 34.1±1.0* |
| Embodiment 2 | 35.0±1.2** |
| Embodiment 3 | 36.1±1.0** |
Note: compared with blank group, ##p < 0.01, compared with model group, p < 0.01 * p < 0.05, * *.
2) influence of the anti-depression Chinese medicament compound extract to reserpine induction experiment mice hypothalamus monoamine levels
It the results are shown in Table described in 5, compared to the blank group compared with positive control drug fluoxetine dose group and anti-depression Chinese medicament compound mention
3 groups of 1 ~ embodiment of object embodiment is taken, the effect of significant antagonism reserpine induction mouse temperature decline is capable of.
The variation (n=10, x ± SEM) of mouse hypothalamus monoamine levels in the experiment of 5 reserpine induction of table
| Group | Da (ng/g) | 5-HT (ng/g) |
| Blank group | 1943.4±214.7 | 2234.1±245.4 |
| Model group | 1094.5±164.4## | 1301.7±104.9## |
| Control group | 1789.7±147.6** | 2091.4±297.4** |
| Embodiment 1 | 1567.4±345.7* | 1678.7±279.7* |
| Embodiment 2 | 1686.7±267.1** | 1789.3±324.7** |
| Embodiment 3 | 1641.4±175.4** | 1986.4±127.4** |
Note: compared with blank group, ##p < 0.01, compared with model group, p < 0.01 * p < 0.05, * *.
This experimental study proves that 3 groups of 1 ~ embodiment of anti-depression Chinese medicament compound extract embodiment being capable of significant antagonism benefit blood
It is flat to induce caused mouse temperature decline, prompt anti-depression Chinese medicament compound extract that there is antidepressant effect, and its control work
With with influence maincenter monoaminergic nerve system it is related.
Claims (2)
1. a kind of Chinese medicinal compound extract with antidepressant activity, it is characterised in that made by ratio of weight and the number of copies by following raw material
At:
Radix Glycyrrhizae 2-5, rhizoma menispermi 5-8, ramulus cinnamomi 8-10, Rhizoma Chuanxiong 1-4；
The preparation method of the above-mentioned Chinese medicinal compound extract with antidepressant activity, comprising the following steps:
(1) Radix Glycyrrhizae, ramulus cinnamomi and Rhizoma Chuanxiong are proportionally deployed, and are placed in container and are extracted with the 10-15 times of 70-80% alcohol reflux measured
3 times, 1-2 hours each, filtering and recycle ethanol extract and being concentrated into room temperature relative density is 1.15-1.20, is spray-dried,
Up to alcohol extracting thing；
(2) rhizoma menispermi decocts 3 times after being impregnated 3-5 hours with 5-10 times of amount, 1-2 hours each, filters and recycle aqueous extract,
Being concentrated into room temperature relative density is 1.05-1.15, is spray-dried to get water extract；It smashs to pieces and to be dried, crushed into fine powder standby
With；
(3) by alcohol extracting thing and water extract be uniformly mixed to get.
2. the Chinese medicinal compound extract with antidepressant activity treats depressive illness drug in preparation as described in claim 1
In application.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724060A (en) * | 2005-07-01 | 2006-01-25 | 成都中医药大学附属医院 | A kind of pharmaceutical composition for the treatment of depression and preparation method thereof |
| CN1883557A (en) * | 2005-06-23 | 2006-12-27 | 上海中医药大学 | Application of 'Guizhi Gancao Longgu Muli' preparation in preparation of medicine for preventing and treating depression |
| CN101129492A (en) * | 2007-07-12 | 2008-02-27 | 天津飞鹰制药有限公司 | Traditional Chinese medicine formulated product for treating melancholia |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1883557A (en) * | 2005-06-23 | 2006-12-27 | 上海中医药大学 | Application of 'Guizhi Gancao Longgu Muli' preparation in preparation of medicine for preventing and treating depression |
| CN1724060A (en) * | 2005-07-01 | 2006-01-25 | 成都中医药大学附属医院 | A kind of pharmaceutical composition for the treatment of depression and preparation method thereof |
| CN101129492A (en) * | 2007-07-12 | 2008-02-27 | 天津飞鹰制药有限公司 | Traditional Chinese medicine formulated product for treating melancholia |
Non-Patent Citations (1)
| Title |
|---|
| 川芎对血管性抑郁症的脑保护作用;谭赛 等;《环球中药学》;20150430;第8卷(第4期);第493页左栏最后1段 |
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