CN104970047A - Liriomyza sativae attractant and application thereof in alfalfa saline-alkali soil planting - Google Patents
Liriomyza sativae attractant and application thereof in alfalfa saline-alkali soil planting Download PDFInfo
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Abstract
The invention provides a liriomyza sativae attractant. The attractant comprises the following components in parts by weight: 35-40 parts of liriomyza sativae sex hormone extract, 25-30 parts of alfalfa extract powder, 7-10 parts of pyrogallol, 5-7 parts of Myricetin, 6-9 parts of quercetin, 12-15 parts of p-tert-butylphenyl salicylate, 20-30 parts of ethyl acetate and 5-8 parts of tebufenozide. The invention also provides application of the attractant in alfalfa saline-alkali soil planting. The liriomyza sativae attractant has the beneficial effects that the attractant provided by the invention has good trapping and killing effects for liriomyza sativae, and the trapping and killing amount of liriomyza sativae reaches 82-150 heads per trap device in 20 days; and the attractant provided by the invention has good trapping and killing effects for snout beetle, and the trapping and killing amount of snout beetle reaches 60-120 heads per trap device in 20 days.
Description
Technical field
The invention provides a kind of liriomyza sativae attractant and the application in the plantation of alfalfa saline land thereof, belong to field of pest control.
Background technology
Liriomyza bryoniae is diptera Agromyzidae, plants the insect of latent fly subfamily, Liriomyza, has recorded kind more than 300 so far, and wherein having 23 kinds to have great economic implications can endanger or take food cultivated plant and ornamental plants.Because their host ranges are wide, it is hidden to cause harm, fertility is strong, harmfulness is large and diffusive transport very fast, some countries and regions are classified as quarantine harmful organisms.
For a long time, people mainly rely on chemical agent and control liriomyza bryoniae population, in Alfalfa Growing, control liriomyza bryoniae is also a major issue, alfalfa is processed into grass meal and uses as the primary raw material of domestic animal manufactured feed, play balanced ration amino acid, abundant vitamin is provided, and good fiber quality element is provided.But because liriomyza bryoniae reproduction speed is fast, create pesticide resistance to chemical agent used, meanwhile, a large amount of Agro-chemicals control liriomyza bryoniae that uses also can cause environmental pollution, thus affects human health.
The deficiency that prior art exists:
The trapping effect of the attractant adopted in current technology to liriomyza bryoniae is bad; The incidence of liriomyza bryoniae insect pest in Alfalfa Growing district and occurring area are very large; In Alfalfa Growing district, the insect pest incidence of weevil and occurring area large.
Summary of the invention
The present invention is the deficiency solving prior art existence, provides a kind of liriomyza sativae attractant and the application in the plantation of alfalfa saline land thereof, to realize following goal of the invention:
(1) liriomyza sativae attractant prepared of the present invention, good to the trapping effect of liriomyza bryoniae, in 20 days, 82-150 head/trapper is reached to the trapping amount of liriomyza bryoniae;
(2) liriomyza sativae attractant prepared of the present invention, good to the trapping effect of weevil, in 20 days, 60-120 head/trapper is reached to the trapping amount of weevil;
(3) liriomyza sativae attractant prepared of the present invention, reduces incidence and the occurring area of liriomyza bryoniae insect pest;
(4) liriomyza sativae attractant prepared of the present invention, reduces incidence and the occurring area of weevil insect pest;
(5) liriomyza sativae attractant prepared of the present invention, slow-release time is long, reaches 45-50 days;
(6) liriomyza sativae attractant of the present invention, in the plantation of alfalfa saline land after application, good to the trapping effect of alfalfa liriomyza bryoniae and weevil, the fresh grass of alfalfa and the output increased of hay; Nitrogen fixing capacity improves.
For solving above technical problem, the technical solution used in the present invention is as follows:
A kind of liriomyza sativae attractant, described attractant, comprises liriomyza bryoniae sex hormone extract, alfalfa extract powder.
Below the further improvement to technique scheme:
Described attractant, also comprises biphenyl 3 phenol, myricetin, quercetin, p-t-butylphenyl salicylate, ethyl acetate, worm hydrazides.
Described attractant, the mass ratio of each component is: liriomyza bryoniae sex hormone extract 35-40 part, alfalfa extract powder 25-30 part, biphenyl 3 phenol 7-10 part, myricetin 5-7 part, quercetin 6-9 part, p-t-butylphenyl salicylate 12-15 part, ethyl acetate 20-30 part, worm hydrazides 5-8 part.
Described alfalfa extract powder is the herb extract of leguminous plant alfalfa, and brown color fine powder, by 80 orders, containing 50% alfalfa saponin.
A kind of preparation method of liriomyza sativae attractant, described method comprises the preparation that attractant lures core carrier, described attractant lures the preparation of core carrier, core carrier will be lured to be placed in analytically pure ethanol soak 40 days, ethanol 10 times to be changed during this, lure core carrier to put to dry under field conditions (factors) by what handle well.
Describedly lure core carrier, containing the isoprene rubber of the butadiene-styrene rubber and 80% (W/W) of 20% (W/W).
Described preparation method also comprises the making that attractant lures core, liriomyza sativae attractant is added drop-wise to prepare lure in core carrier, to be that 40-50mg/ is each lure core to dripping quantity.
The application of liriomyza sativae attractant in the plantation of alfalfa saline land, described application, comprises the use of liriomyza sativae attractant, is lured by liriomyza sativae attractant core to be loaded in slowly-releasing bottle, then by bottled for slowly-releasing on trapper, trapper overhead 0.8m bottom.
Described trapper interval about 45m.
compared with prior art, having following beneficial effect is in the present invention:
(1) liriomyza sativae attractant prepared of the present invention, good to the trapping effect of liriomyza bryoniae, on 20-July 10 June, 82-150 head/trapper is reached to the trapping amount of liriomyza bryoniae;
(2) liriomyza sativae attractant prepared of the present invention, good to the trapping effect of weevil, on 10-June 30 June, 60-120 head/trapper is reached to the trapping amount of weevil;
(3) liriomyza sativae attractant prepared of the present invention, can reduce incidence and the occurring area of liriomyza bryoniae insect pest, 20-July 10 June, the insect pest incidence of liriomyza bryoniae is 8-13%, and the insect pest occurrence area of liriomyza bryoniae is 7-15%;
(4) liriomyza sativae attractant prepared of the present invention, can reduce incidence and the occurring area of weevil insect pest, 10-June 30 June, the incidence of nose pests is 3-8%, and the occurring area of nose pests is 5-10%;
(5) liriomyza sativae attractant prepared of the present invention, slow-release time is long, reaches 45-50 days;
(6) liriomyza sativae attractant of the present invention, in the plantation of alfalfa saline land after application, good to the trapping effect of alfalfa liriomyza bryoniae and weevil, the fresh grass of alfalfa and the output increased of hay, nitrogen fixing capacity improves: after planting 2 years, every mu of green forage yield at 4000-5000 kilogram, hay yield 1050 ~ 1100 kilograms; Every mu of alfalfa can fix nitrogen 22 kilograms every year from air, is equivalent to 67 kilograms of ammonium nitrate.
Embodiment
Below the preferred embodiments of the present invention are described, should be appreciated that preferred embodiment described herein is only for instruction and explanation of the present invention, is not intended to limit the present invention.
embodiment 1 one kinds of liriomyza sativae attractants
the mass ratio of each component of attractant is:
Liriomyza bryoniae sex hormone extract 35 parts, 20 parts, alfalfa extract powder, biphenyl 3 phenol 6 parts, myricetin 3 parts, quercetin 4 parts, p-t-butylphenyl salicylate 9 parts, 20 parts, ethyl acetate, worm hydrazides 5 parts.
the requirement of described alfalfa extract powder is:
For the herb extract of leguminous plant alfalfa; brown color fine powder; by 80 orders, containing 50% alfalfa saponin, described alfalfa herb; for alfalfa mixing hay; average crude protein content 18.9%, crude fat 3.37%, nitrogen free extract 29.97%; raw fiber 26.80%, ash content 7.03%.
the preparation method of described liriomyza bryoniae sex hormone extract:
(1) dry, grind
Collect the budding female insect of liriomyza bryoniae, drying polypide to moisture content is 6-7%, is milled to 65-80 order;
(2) ultrasonic process, filtration
Amount of alcohol added is 10 times of body weight, soaks worm powder 30h, and through ultrasonic vibration 5-6 time after stirring, each 4min, filters, reservation filtrate;
(3) merging filtrate
Residue stirs after adding the alcohol-pickled 3.5-4h of 3 times of weight again, ultrasonic vibration 5-6 time, each 4min, filters; Filtrate is with 2) filtrate that obtains of step merges; Residue repeats 2 again), 3) step extract 2-3 time, all filtrate is merged;
(4) refluxing extraction
Add in filtrate after merging and account for protozoon soma grain weight amount 7-8 alcohol doubly, backflow 6h, heating recovery of alcohol distillation, the residue after distillation retains;
(5) boil, filter, concentrate
In distillation residue, add the water of 4-5 times of volume, stir to boil in boiling water bath and boil 7-8min, be then cooled to room temperature, natural filtration; Carry out boiling again boiling 7-8min, cooling, natural filtration; Repeat 2-3 time; Gained filtrate is concentrated into gravity Baume 1.2-1.5 ° of B é;
(6) add alcohol, leave standstill
Add isopyknic alcohol, leave standstill 6-12h;
(7) precipitation is gone
Discard precipitation after filtration, the filtrate obtained is the sex hormone solution of liriomyza bryoniae.
embodiment 2 one kinds of liriomyza sativae attractants
the mass ratio of each component of attractant is:
Liriomyza bryoniae sex hormone extract 37 parts, 22 parts, alfalfa extract powder, biphenyl 3 phenol 7 parts, myricetin 4 parts, quercetin 6 parts, p-t-butylphenyl salicylate 11 parts, 25 parts, ethyl acetate, worm hydrazides 6 parts.
the requirement of described alfalfa extract powder is:
Adopt the requirement of embodiment 1 pair of alfalfa extract powder.
the preparation method of described liriomyza bryoniae sex hormone extract:
The preparation method of embodiment 1 is adopted to prepare sex hormone extract.
embodiment 3 one kinds of liriomyza sativae attractants
the mass ratio of each component of attractant is:
Liriomyza bryoniae sex hormone extract 40 parts, 25 parts, alfalfa extract powder, biphenyl 3 phenol 8 parts, myricetin 5 parts, quercetin 8 parts, p-t-butylphenyl salicylate 13 parts, 30 parts, ethyl acetate, worm hydrazides 8 parts.
the requirement of described alfalfa extract powder is:
Adopt the requirement of embodiment 1 pair of alfalfa extract powder.
the preparation method of described liriomyza bryoniae sex hormone extract
The preparation method of embodiment 1 is adopted to prepare sex hormone extract.
the preparation method of embodiment 4 one kinds of liriomyza sativae attractants
(1) dissolving of each composition of attractant
According to the mass ratio of each component described in embodiment 1-3, liriomyza bryoniae sex hormone extract, alfalfa extract powder, biphenyl 3 phenol, myricetin, quercetin, worm hydrazides, p-t-butylphenyl salicylate are dissolved in ethyl acetate, stand-by.
(2) attractant lures the preparation of core carrier
Lure core carrier to adopt rubber to lure core carrier, described rubber lures the isoprene rubber containing the butadiene-styrene rubber and 80% (W/W) of 20% (W/W) in core carrier; Core carrier will be lured to be placed in analytically pure ethanol soak 40 days, ethanol 10 times during this, will be changed, and lure core carrier to put to dry under field conditions (factors) by what handle well, install in sealed plastic bag seal for subsequent use.
(3) attractant lures the making of core
The attractant prepared is added drop-wise to luring of preparing in core carrier, to be that 40-50mg/ is each lure core to dripping quantity, makes attractant of the present invention and lure core, pack, 4 DEG C of refrigerations.
the application of the above-mentioned liriomyza sativae attractant of embodiment 5 in the plantation of alfalfa saline land
(1) selection in saline land
Low-grade alkalization soil, pH is 8.5-8.8, basicity 5-10%, and salt content is 0.6-0.8%, and soil total gap rate is 21-22%, and ventilative hole is 3-5%.
(2) the using of soil conditioner
Use soil conditioner, carry out alkaline land improving.
The mass ratio of each component of modifying agent is:
Potassium feldspar 7-9 part, hydrolyzed polyacrylonitrile 2-4 part, asparagus slag 1-2 part, boric acid 3-4 part, fulvic acid 12-13 part, charcoal 6-7 part, polyvinyl alcohol 5-6 part, polymeric amino acid 3-4 part, addicted to salt methane backeria 0.2-0.4 part, water-loss reducer 0.2-0.5 part.
(3) wholely, apply fertilizer to the subsoil
Ploughed deeply in soil, every mu of organic fertilizer 1500 ~ 2500 kilograms, superphosphate 20 ~ 30 kilograms is base fertilizer, carries out afterwards meticulously requiring Land leveling wholely, and soil block is in small, broken bits, without weeds, and good conditions for soil moisture, before sowing, then row shallow plowing.
(4) seed selection, seed treatment
ABT root-inducing powder (preferably No. 4) is made into the solution of 100mg root-inducing powder/kg water, alfalfa seed is put and soaks in the solution, the preferred 3h of soak time; Microbial inoculum containing rhizobium melioti is joined in sticker, after stirring, then adds alfalfa seed, repeatedly stir, make each seed is all stained with microbial inoculum, the mass ratio of seed and microbial inoculum is preferably 1:11; The alfalfa seed obtained puts into seed-coating machine, repeatedly shakes mixing through seed-coating machine, makes each seed all wrap one deck coating material, finally seed is put in ventilation, dry in the shade stand-by.
(5) sow
Sowing time selects mid-April, and plant mode drilling, seeding quantity is 1.5-2 kg/acre, and sowing depth is 1-2 centimetre.
(6) use of liriomyza sativae attractant
Between planting season, note the control of insect pest, lured by liriomyza sativae attractant core to be loaded in slowly-releasing bottle, then by bottled for slowly-releasing on trapper, trapper is suspended on the surrounding of growing area, trapper overhead 0.8m, each trapper interval about 45m bottom.
(7) irrigate
Irrigate in time, maintenance soil moisture content is 70-75%.
A. irrigate seedling stage:
The mode of employing brackish water, fresh water rotation flow is irrigated, and rotation flow number of times is than being irrigate once for 1:3, every 8-10 days, and each irrigation volume is 360-400m3/ hectare; The salinity of described fresh water is less than 1g/L, and described brackish water salinity is 2.6-3.2g/L, pH is 7.5-7.8, and salt content is 0.08-0.12%, and sodium salt content accounts for the 41-42% of total salt content.
B. irrigate mid-term:
The mode of employing brackish water, fresh water rotation flow is irrigated, and rotation flow number of times is than being irrigate once for 1:2, every 7-8 days, and each irrigation volume is 460-520m3/ hectare;
The salinity of described fresh water is less than 1g/L; Described brackish water salinity is 3.4-3.8g/L, pH is 7.5-7.6, and salt content is 0.12-0.15%, and sodium salt content accounts for the 30-34% of total salt content.
C. animated period is irrigated:
The mode of employing rich trace elements brackish water, fresh water rotation flow is irrigated, and rotation flow number of times is than being irrigate once for 1:2, every 4-6 days, and each irrigation volume is 550-650m3/ hectare;
The salinity of described fresh water is less than 1g/L;
The preparation of described rich trace elements brackish water:
Get trace element fertilizer 8kg to be dissolved in 1000L brackish water, mix, obtained rich trace elements brackish water;
Described trace element fertilizer comprises boric acid 2-4%, ammonium molybdate 5-8%, chelated zinc 7-9%, manganese chloride 12-16%, chelated copper 1-2%, ammonium chloride 18-21%, chelated iron 10-14%;
The salinity of described brackish water is 2.4-2.6g/L, pH is 7.8-8.0, and salt content is 0.07-0.08%, and sodium salt content accounts for the 36-42% of total salt content.
(8) manage
Sowing clover then, seedling stage wants timely weeding 1-2 time, and cradle as best period with initial bloom stage, within 1 year, can cradle 2 ~ 4 times, many persons can cradle 5 ~ 6 times, stay raft height with 3-5 centimetre as well.
comparative examples
Adopt the method for embodiment 5, only change the use step of liriomyza sativae attractant, this step is changed into: do not let alone what attractant in slowly-releasing bottle and lure core, by bottled for slowly-releasing on trapper, trapper is suspended on the surrounding of growing area, trapper overhead 0.8m, each trapper interval about 45m bottom.
result of the test:
(1) add up liriomyza sativae attractant in 20-July 10 June, to the trapping effect of liriomyza bryoniae, incidence, the occurring area of liriomyza bryoniae insect pest in statistics alfalfa, in table 1; Statistics liriomyza sativae attractant is in 10-June 30 June, and to the trapping effect of alfalfa weevil, incidence, the occurring area of weevil insect pest in statistics alfalfa, in table 1.
The trapping effect of table 1 liriomyza sativae attractant
Visible, embodiment 2 is preferred embodiments.
(2) beet webworm of the present invention and chafer attractant, slow-release time is long, reaches 45-50 days, can prevent and treat liriomyza bryoniae and weevil the long period.
(3) after liriomyza sativae attractant of the present invention is applied in the plantation of the saline land of alfalfa, good to the trapping effect of liriomyza bryoniae and weevil, after alfalfa results, measure and add up the index of correlation of alfalfa, as follows:
Alfalfa Root System is flourishing: main root embedded depth 7-10 rice;
The fresh grass of alfalfa and the output increased of hay: after planting 2 years, every mu of green forage yield at 4000-5000 kilogram, hay yield 1050 ~ 1100 kilograms;
In alfalfa blade, protein content improves: the protein content of alfalfa-leaves accounts for 85% of overall strain, leaf protein (ALP) is pulverized by the clover of in good time gathering in, squeeze, solidify, separate out and dry and protein concentrates that is that formed, wherein crude protein content is 62%%, raw fiber 2.5%, the protein content in alfalfa leaf protein reaches the protein content in dregs of beans and fish meal substantially.
The nitrogen fixing capacity of alfalfa improves: the root nodule of alfalfa can fix the nitrogen in air, increase soil fertility, the alfalfa grasslands of 2 years, every mu of root amount fresh weight can reach 3000 kilograms, in every mu of root stubble about nitrogenous 18 kilograms, 2.5 kilograms, full phosphorus, 6.8 kilograms, full potassium, nitrogen 22 kilograms can be fixed every year from air for every mu, be equivalent to 67 kilograms of ammonium nitrate.
4) adopt the method for embodiment 5, Alfalfa Growing is after 2 years, and soil pH is reduced to 7.3-7.5, soil salt content is reduced to 0.2-0.3%, alkalization of soils degree is reduced to 1.4-1.5%, and the overall porosity of soil is increased to 51-53%, and the ventilative hole of soil is increased to as 14-15%.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although with reference to previous embodiment to invention has been detailed description, for a person skilled in the art, it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein portion of techniques feature.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (9)
1. a liriomyza sativae attractant, is characterized in that: described attractant, comprise liriomyza bryoniae sex hormone extract,
Alfalfa extract powder.
2. a kind of liriomyza sativae attractant according to claim 1, is characterized in that: described attractant, also comprises biphenyl 3 phenol, myricetin, quercetin, p-t-butylphenyl salicylate, ethyl acetate, worm hydrazides.
3. a kind of liriomyza sativae attractant according to claim 1, it is characterized in that: described attractant, the mass ratio of each component is: liriomyza bryoniae sex hormone extract 35-40 part, alfalfa extract powder 25-30 part, biphenyl 3 phenol 7-10 part, myricetin 5-7 part, quercetin 6-9 part, p-t-butylphenyl salicylate 12-15 part, ethyl acetate 20-30 part, worm hydrazides 5-8 part.
4. a kind of liriomyza sativae attractant according to claim 1, is characterized in that: described alfalfa extract powder, is the herb extract of leguminous plant alfalfa, and brown color fine powder, by 80 orders, containing 50% alfalfa saponin.
5. the preparation method of a liriomyza sativae attractant, it is characterized in that: described method comprises the preparation that attractant lures core carrier, described attractant lures the preparation of core carrier, core carrier will be lured to be placed in analytically pure ethanol soak 40 days, ethanol 10 times to be changed during this, lure core carrier to put to dry under field conditions (factors) by what handle well.
6. the preparation method of a kind of liriomyza sativae attractant according to claim 5, is characterized in that: described in lure core carrier, containing the isoprene rubber of the butadiene-styrene rubber and 80% (W/W) of 20% (W/W).
7. the preparation method of a kind of liriomyza sativae attractant according to claim 5, it is characterized in that: described preparation method also comprises the making that attractant lures core, liriomyza sativae attractant is added drop-wise to prepare lure in core carrier, to be that 40-50mg/ is each lure core to dripping quantity.
8. the application of liriomyza sativae attractant in the plantation of alfalfa saline land, it is characterized in that: described application, comprise the use of liriomyza sativae attractant, lured by liriomyza sativae attractant core to be loaded in slowly-releasing bottle, again by bottled for slowly-releasing on trapper, trapper overhead 0.8m bottom.
9. the application of a kind of liriomyza sativae attractant according to claim 8 in the plantation of alfalfa saline land, is characterized in that: described trapper interval about 45m.
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|---|---|---|---|---|
| CN116616316A (en) * | 2023-07-19 | 2023-08-22 | 山东浏园生态农业股份有限公司 | Attractant for tea tree pests and preparation method and application thereof |
| CN116616316B (en) * | 2023-07-19 | 2023-09-15 | 山东浏园生态农业股份有限公司 | Attractant for tea tree pests and preparation method and application thereof |
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Application publication date: 20151014 |