Summary of the invention
The object of the present invention is to provide a kind of conserving case detecting food paste, to solve the problems of the technologies described above.
Technical problem to be solved by this invention realizes by the following technical solutions:
A kind of conserving case detecting food paste, it is characterized in that: comprise insulation box body and be placed in the sample card in insulation box body, object disposing platform has been horizontally disposed with in described insulation box body, object disposing platform will be separated into upper and lower two cavitys in insulation box body, object disposing platform is provided with limbers, be communicated with by limbers between upper and lower two cavitys, the lower chamber of described insulation box body is connected temperature adjustment water tank by water inlet pipe with rising pipe, heating unit is provided with in described temperature adjustment water tank, described water inlet pipe and rising pipe are provided with control valve, described water inlet pipe is provided with water circulating pump,
Described sample card comprises substrate and upper cover plate, the upper surface of described substrate and the lower surface of upper cover plate are glass planar, the upper end of described substrate offers culture tank, oiliness coating is provided with around the upper shed of culture tank, the edge, upper end of described substrate is provided with chimb upwards, described upper cover plate is covered on the upper end of substrate, has gap between the edge of upper cover plate and the chimb of substrate.
Described insulation box body adopts water bath heat preservation, and the horizontal plane in insulation box body is higher than the liquid level of nutrient solution in culture tank.
Described substrate offers multiple culture tank, and can add detected sample and nutrient solution in each culture tank, multiple culture tank carries out test experience simultaneously simultaneously, and detected result is more reliable, and testing staff observes more directly perceived, easily judges.
Described upper cover plate is transparent glass, is convenient to testing staff and observes judgement detected result.
Sample to be detected and nutrient solution add in culture tank, upper cover plate and substrate cover, the upper surface of substrate and the lower surface of upper cover plate are that glass planar can well be fitted, then add water in the gap between the edge and substrate chimb of upper cover plate with dropper, with water by substrate and upper cover plate involution, moisture film is formed between substrate and upper cover plate, and the air of substrate and upper cover plate is all discharged, because scribble oiliness coating around the upper shed of culture tank, moisture film can not be crossed this oiliness coating and enter in culture tank, if and culture tank in continue produce gas, then gas will enter between substrate and upper cover plate, moisture film is arranged, bubble is one by one formed between substrate and upper cover plate, be convenient to very much observe.
In food paste, the detection method of microorganism, comprises the steps:
A () is got food paste to be detected and is put into beaker, add the sterilized water of 10 times, be fully uniformly mixed in beaker, makes food paste and detects liquid, food paste is detected liquid and be placed in the gnotobasis 6-8 hour that temperature is 30-35 DEG C;
B food paste that () gets 1ml detects liquid and adds in the culture tank of sample card, then adds nutrient solution in culture tank, covers the upper cover plate of sample card, and with water seal (gap between the substrate of closed sample card and upper cover plate);
C sample card after water seal is placed in constant temperature culture in insulation box body by (), temperature 32-34 DEG C, cultivates 36 hours;
Whether create bubble in the water seal face of (d) observation sample card, create bubble and then proved that microorganism exists, do not produce bubble and then prove there is no microorganism;
Main containing following material component in often liter of nutrient solution:
Green tea powder not only can provide Carbon and nitrogen sources, meet the demand of bacterial growth, and nutrient solution potential of hydrogen can be regulated, along with the breeding of microorganism, the pH value of nutrient solution can change because of the breeding of microorganism, and green tea powder acidic substance stripping in early stage is very fast, and later stage alkaline matter starts stripping, can play regulating effect to the potential of hydrogen of nutrient solution.Adding of tobacco leaf powder can avoid nutrient solution to become peracidity, simultaneously also can provide Carbon and nitrogen sources, and animal tallow, golden cicada powder, gelatin provide sufficient Carbon and nitrogen sources for microorganism growth; Sodium-chlor can maintain balanced osmotic pressure; Lactose is the fermentable carbohydrate of coliform; Potassium primary phosphate and dipotassium hydrogen phosphate are buffer reagents.
The invention has the beneficial effects as follows:
Conserving case provided by the invention, for detecting the microorganism in food paste, this conserving case adopts the mode of water bath heat preservation to be incubated sample card, for microbial growth provides adapt circumstance, can carry out organizing test experience, and detected result can be observed simultaneously more simultaneously, only need can determine whether that microorganism exists by whether producing bubble between observation upper cover plate and substrate, this detection method is simple to operate, comparatively directly perceived to the judgement of detected result, is not easy to occur erroneous judgement.
Embodiment
The technique means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiments and the drawings, set forth the present invention further, but following embodiment being only the preferred embodiments of the present invention, and not all.Based on the embodiment in embodiment, those skilled in the art under the prerequisite not making creative work obtain other embodiment, all belong to protection scope of the present invention.
As Fig. 1, shown in Fig. 2, a kind of conserving case detecting food paste, the sample card 3 comprising insulation box body 2 and be placed in insulation box body 2, object disposing platform 1 has been horizontally disposed with in insulation box body 2, object disposing platform 1 will be separated into upper and lower two cavitys in insulation box body 2, object disposing platform 1 is provided with limbers, the warm water bath of lower chamber can be communicated with upper cavity by limbers, the lower chamber of insulation box body 2 is connected temperature adjustment water tank 6 by water inlet pipe 8 with rising pipe 5, heating unit 7 is provided with in temperature adjustment water tank 6, water inlet pipe 8 and rising pipe 5 are provided with control valve 4, water inlet pipe 8 is provided with water circulating pump 9,
Sample card 3 comprises substrate 33 and upper cover plate 31, the upper surface of substrate 33 and the lower surface of upper cover plate 31 are glass planar, the upper end of substrate 33 offers the culture tank 32 that cross section is circular arc, oiliness coating 36 is provided with around the upper shed of culture tank 32, the edge, upper end of substrate 33 is provided with chimb 35 upwards, upper cover plate 31 is covered on the upper end of substrate 33, has gap 34 between the edge of upper cover plate 31 and the chimb 35 of substrate 33.
More than show and describe ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets is only preference of the present invention; be not used for limiting the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.