CN104962464B - A kind of conserving case for detecting food paste - Google Patents

A kind of conserving case for detecting food paste Download PDF

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Publication number
CN104962464B
CN104962464B CN201510244393.5A CN201510244393A CN104962464B CN 104962464 B CN104962464 B CN 104962464B CN 201510244393 A CN201510244393 A CN 201510244393A CN 104962464 B CN104962464 B CN 104962464B
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Prior art keywords
substrate
cover plate
food paste
upper cover
incubation chamber
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CN104962464A (en
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邵栋梁
高柱
程莹
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Anhui Zhong Qing Inspection Co Ltd
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Anhui Zhong Qing Inspection Co Ltd
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides a kind of conserving case for detecting food paste, it is related to technical field of microbial detection in food paste, including insulation box body and the sample card being placed in incubation chamber body, being horizontally disposed with the incubation chamber body has object disposing platform, object disposing platform will be separated into upper and lower two cavitys in incubation chamber body, limbers is provided with object disposing platform, connected by limbers between upper and lower two cavitys, the lower chamber of the insulation box body connects temperature adjustment water tank by water inlet pipe and outlet pipe, heater is provided with the temperature adjustment water tank, control valve is provided with the water inlet pipe and outlet pipe, water circulating pump is provided with the water inlet pipe.The present invention to the microorganism in food paste for detecting, only need to by observe whether produced between upper cover plate and substrate bubble can determine whether there is microorganism presence, the detection method is simple to operate, and the judgement to testing result is more directly perceived, it is not easy to erroneous judgement occur.

Description

A kind of conserving case for detecting food paste
Technical field
The present invention relates to technical field of microbial detection in food paste, more particularly to a kind of conserving case for detecting food paste.
Background technology
Food paste is vulnerable to the pollution of microorganism during processing, transport and storing.Food hygiene supervision is needed to food Microorganism is detected in product sauce.
In food paste production process, along with detecting whether there is quality of the certain micro-organisms to ensure final products. In the prior art, be generally required for very those skilled in the art to observe and explain assay, thus need it is a kind of more Method that is convenient, intuitively, accurately being detected to microorganism in food paste.
The content of the invention
It is an object of the invention to provide a kind of conserving case for detecting food paste, to solve above-mentioned technical problem.
The technical problems to be solved by the invention are realized using following technical scheme:
A kind of conserving case for detecting food paste, it is characterised in that:Including insulation box body and the sample being placed in incubation chamber body Card, being horizontally disposed with the incubation chamber body has object disposing platform, and object disposing platform will be separated into upper and lower two cavitys, put in incubation chamber body Limbers is provided with thing platform, is connected by limbers between upper and lower two cavitys, the lower chamber of the insulation box body passes through Water inlet pipe and outlet pipe connection temperature adjustment water tank, are provided with heater, on the water inlet pipe and outlet pipe in the temperature adjustment water tank Control valve is provided with, water circulating pump is provided with the water inlet pipe;
The sample card includes substrate and upper cover plate, and the upper surface of the substrate and the lower surface of upper cover plate are flat for glass Face, the upper end of the substrate offers culture tank, and oiliness coating, the upper end of the substrate are provided with around the upper shed of culture tank Edge is provided with upward chimb, and the upper cover plate is covered on the upper end of substrate, between the edge of upper cover plate and the chimb of substrate There is gap.
The insulation box body is incubated using water-bath, liquid level of the horizontal plane in incubation chamber body higher than nutrient solution in culture tank.
Multiple culture tanks are offered on the substrate, detected sample and nutrient solution can be simultaneously added in each culture tank, Multiple culture tanks carry out test experience simultaneously, and testing result is relatively reliable, and testing staff's observation is more directly perceived, easily determines.
The upper cover plate is clear glass, is easy to testing staff to observe and judges testing result.
Sample to be detected and nutrient solution are added in culture tank, and upper cover plate is covered with substrate, the upper surface of substrate and upper lid The lower surface of plate for glass planar can be very good laminating, then between the edge and substrate chimb with dropper to upper cover plate between Added water in gap, sealed substrate and upper cover plate with water, form moisture film between substrate and upper cover plate, and by substrate and the sky of upper cover plate Gas is all discharged, because scribbling oiliness coating around the upper shed of culture tank, moisture film will not cross the oiliness coating and enter culture In groove, and if persistently producing gas in culture tank, then gas will enter between substrate and upper cover plate, and moisture film is arranged, Bubble one by one is formed between substrate and upper cover plate, observation is highly convenient for.
The detection method of microorganism, comprises the following steps in food paste:
A () takes food paste to be detected and is put into beaker, to the sterilized water that 10 times are added in beaker, be thoroughly mixed, Food paste detection liquid is made, food paste detection liquid is placed in 6-8 hours in the gnotobasis that temperature is 30-35 DEG C;
B () is taken in the culture tank of food paste detection liquid addition sample card of 1ml, then to nutrient solution is added in culture tank, cover The upper cover plate of upper sample card, and with water seal (closing sample card substrate and upper cover plate between gap);
C () sample after water seal is placed in incubated in incubation chamber body, 32-34 DEG C of temperature, cultivate 36 hours;
Whether bubble is generated in the water seal face of (d) observation sample card, generating bubble then proves with the presence of microorganism, does not have Having generation bubble then proves no microorganism;
Following material component is mainly contained in every liter of nutrient solution:
Green tea powder can not only provide carbon source and nitrogen source, meet the demand of bacterial growth, and can adjust nutrient solution acid Basicity, with the breeding of microorganism, the pH value of nutrient solution can change because of the breeding of microorganism, and green tea powder early stage is sour Property material dissolution it is very fast, and later stage alkaline matter starts dissolution, can play adjustment effect to the acid-base value of nutrient solution.Tobacco leaf powder Addition can avoid nutrient solution into peracidity, while can also provide carbon source and nitrogen source, animal tallow, golden cicada powder, gelatin are micro- Biological growth provides sufficient carbon source and nitrogen source;Sodium chloride can maintain osmotic pressure in a balanced way;Lactose is fermentable coliform Carbohydrate;Potassium dihydrogen phosphate and dipotassium hydrogen phosphate are buffers.
The beneficial effects of the invention are as follows:
The conserving case that the present invention is provided, for being detected to the microorganism in food paste, the conserving case is protected using water-bath The mode of temperature is incubated to sample card, for the growth of microorganism provides suitable environment, can simultaneously carry out multigroup test experience, And testing result can be observed simultaneously, need to be by observe whether produced between upper cover plate and substrate bubble and can determine that only No with the presence of microorganism, the detection method is simple to operate, and the judgement to testing result is more directly perceived, it is not easy to erroneous judgement occur.
Brief description of the drawings
Fig. 1 is structural representation of the invention;
Fig. 2 is the schematic top plan view of sample card.
Specific embodiment
In order that technological means, creation characteristic, reached purpose and effect that the present invention is realized are easy to understand, tie below Specific embodiments and the drawings are closed, the present invention is expanded on further, but following embodiments are only the preferred embodiments of the present invention, not All.Based on the embodiment in implementation method, those skilled in the art are obtained on the premise of creative work is not made Other embodiments, belong to protection scope of the present invention.
As shown in Figure 1 and Figure 2, a kind of conserving case for detecting food paste, including be incubated box body 2 and be placed in insulation box body 2 Sample card 3, being horizontally disposed with insulation box body 2 has object disposing platform 1, and object disposing platform 1 will be incubated in box body 2 and be separated into upper and lower two chambers Body, is provided with limbers on object disposing platform 1, the tepidarium of lower chamber can connect upper cavity by limbers, insulation box body 2 Lower chamber is connected in temperature adjustment water tank 6, temperature adjustment water tank 6 by water inlet pipe 8 and outlet pipe 5 and is provided with heater 7, the He of water inlet pipe 8 Control valve 4 is provided with outlet pipe 5, water circulating pump 9 is provided with water inlet pipe 8;
Sample card 3 includes substrate 33 and upper cover plate 31, and the upper surface of substrate 33 and the lower surface of upper cover plate 31 are flat for glass Face, the upper end of substrate 33 offers the culture tank 32 that section is circular arc, and oiliness painting is provided with around the upper shed of culture tank 32 Layer 36, the upper end edge of substrate 33 is provided with upward chimb 35, and upper cover plate 31 is covered on the upper end of substrate 33, upper cover plate 31 There is gap 34 between the chimb 35 of edge and substrate 33.
General principle of the invention, principal character and advantages of the present invention has been shown and described above.The technology of the industry It should be appreciated that the present invention is not limited to the above embodiments, described in above-described embodiment and specification is only the present invention to personnel Preference, be not intended to limit the present invention, without departing from the spirit and scope of the present invention, the present invention also have it is various Changes and improvements, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by institute Attached claims and its equivalent thereof.

Claims (4)

1. it is a kind of detect food paste conserving case, it is characterised in that:Including insulation box body and the sample card being placed in incubation chamber body, Being horizontally disposed with the incubation chamber body has object disposing platform, and object disposing platform will be separated into upper and lower two cavitys, glove in incubation chamber body Limbers is provided with platform, between upper and lower two cavitys by limbers connect, it is described insulation box body lower chamber pass through into Water pipe and outlet pipe connection temperature adjustment water tank, are provided with heater, on the water inlet pipe and outlet pipe in the temperature adjustment water tank Control valve is provided with, water circulating pump is provided with the water inlet pipe;
The sample card includes substrate and upper cover plate, and the upper surface of the substrate and the lower surface of upper cover plate are glass planar, institute The upper end for stating substrate offers culture tank, and oiliness coating, the upper end edge of the substrate are provided with around the upper shed of culture tank Upward chimb is provided with, the upper cover plate is covered on the upper end of substrate, between having between the edge of upper cover plate and the chimb of substrate Gap, the culture tank is arc groove.
2. it is according to claim 1 detection food paste conserving case, it is characterised in that:The insulation box body is protected using water-bath Temperature, liquid level of the horizontal plane higher than nutrient solution in culture tank in incubation chamber body.
3. it is according to claim 1 detection food paste conserving case, it is characterised in that:Multiple trainings are offered on the substrate Support groove.
4. it is according to claim 1 detection food paste conserving case, it is characterised in that:The upper cover plate is clear glass.
CN201510244393.5A 2015-07-07 2015-07-07 A kind of conserving case for detecting food paste Active CN104962464B (en)

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CN104962464B true CN104962464B (en) 2017-06-23

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105671121A (en) * 2016-03-10 2016-06-15 山东五洲检测有限公司 Detection method of microorganisms in milk

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1100468A (en) * 1993-09-14 1995-03-22 牟自修 Rapid bacteria-checking liquid
CN202219174U (en) * 2011-07-20 2012-05-16 北京英茂药业有限公司 Constant-temperature water bath kettle
CN203329405U (en) * 2013-05-13 2013-12-11 安徽天恒药业有限公司 Multipurpose constant temperature ultrasound water bath tank
CN103667055A (en) * 2012-09-25 2014-03-26 索尼公司 Culture container, culture observation apparatus and culture observation method
CN203700358U (en) * 2014-03-06 2014-07-09 张秀花 Disposable rapid microorganism culture and detection dish
CN203820790U (en) * 2014-05-08 2014-09-10 周巍 Inspection tube for counting gas-producingmicroorganisms
CN104351748A (en) * 2014-10-23 2015-02-18 赵聪 Production method of nutritious canned hericium erinaceus

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1100468A (en) * 1993-09-14 1995-03-22 牟自修 Rapid bacteria-checking liquid
CN202219174U (en) * 2011-07-20 2012-05-16 北京英茂药业有限公司 Constant-temperature water bath kettle
CN103667055A (en) * 2012-09-25 2014-03-26 索尼公司 Culture container, culture observation apparatus and culture observation method
CN203329405U (en) * 2013-05-13 2013-12-11 安徽天恒药业有限公司 Multipurpose constant temperature ultrasound water bath tank
CN203700358U (en) * 2014-03-06 2014-07-09 张秀花 Disposable rapid microorganism culture and detection dish
CN203820790U (en) * 2014-05-08 2014-09-10 周巍 Inspection tube for counting gas-producingmicroorganisms
CN104351748A (en) * 2014-10-23 2015-02-18 赵聪 Production method of nutritious canned hericium erinaceus

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Denomination of invention: Preservation box for food sauce detection

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