CN104958249A - Antrodia cinnamomea cream - Google Patents
Antrodia cinnamomea cream Download PDFInfo
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- CN104958249A CN104958249A CN201510432169.9A CN201510432169A CN104958249A CN 104958249 A CN104958249 A CN 104958249A CN 201510432169 A CN201510432169 A CN 201510432169A CN 104958249 A CN104958249 A CN 104958249A
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- antrodia camphorata
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- emulsifiable paste
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Abstract
The invention discloses antrodia cinnamomea cream, comprising the following components: 0.5-2% of antrodia cinnamomea extract, soybean phospholipid, 0.1-0.3% of antioxidant, pH buffering agent for keeping the pH value at 5-7.5, 0.05-0.1% of antibacterial preservative, 3-10% of humectant, 20-30% of oil phase component and the balance of water, wherein the weight ratio of the soybean phospholipid to the antrodia cinnamomea extract is (2-3): 1.
Description
Technical field
The present invention relates to a kind of Antrodia Camphorata extract emulsifiable paste.
Background technology
Antrodia Camphorata, be called for short " Antrodia camphorata ", formal name used at school is Antrodia cinnamomea, is the medicinal fungi of a kind of growth on Taiwan Cinnamomum kanahirai hay tree.Antrodia Camphorata has removing toxic substances and protects the liver, anticancer, reinforced immunological, antiallergic, the effects such as blood fat reducing, can take as health product, compared with Ganoderma, the triterpene content of Antrodia Camphorata sporophore is higher, there is stronger health-care effect, the effect improving skin quality can be played in the Antrodia Camphorata extract outer used time, but find, as the triterpenes as main active in Antrodia Camphorata extract, there is certain phototoxicity under study for action, limit its application in externally applied product, therefore provide a kind of overcome its phototoxic Antrodia Camphorata externally applied product become in prior art need solve problem.
Summary of the invention
For solving the problems of the technologies described above, the object of this invention is to provide a kind of Antrodia Camphorata emulsifiable paste, we find to adopt soybean phospholipid to coordinate with certain proportion with Antrodia Camphorata extract under study for action, the phototoxicity of can reduce Antrodia Camphorata extract significantly used time outside, and provide a kind of Antrodia Camphorata emulsifiable paste based on this discovery.
The invention provides a kind of Antrodia Camphorata emulsifiable paste, described Antrodia Camphorata emulsifiable paste is made up of following component
Antrodia Camphorata extract 0.5 ~ 2%; Soybean phospholipid, the weight ratio of soybean phospholipid and Antrodia Camphorata extract is 2-3:1;
Antioxidant 0.1% ~ 0.3%, remains on the pH buffer agent of 5 ~ 7.5, antibiotic antiseptic 0.05% ~ 0.1%, wetting agent 3%-10%, oil-phase component 20%-30% by pH value, and the water of surplus;
Described antioxidant is selected from EDTA-2Na, EDTA-CaNa
2, one or more in tocopherol;
Preferred EDTA-CaNa
2.
Described antibiotic antiseptic is selected from one or more in methyl hydroxybenzoate, ethyl hydroxybenzoate, propylparaben.
Described pH buffer agent is selected from phosphoric acid/phosphate buffer, acetic acid/acetate buffer, citric acid/citrate buffer agent, preferably phosphoric acid/phosphate buffer
Described wetting agent is selected from one or more in glycerol, propylene glycol, sorbitol, preferred glycerol.
Described oil-phase component by as the hexadecanol of higher alcohol and/or octadecanol, as the vaseline of consistency modifiers and/or liquid paraffin and emulsifying agent composition; Described emulsifying agent is selected from glyceryl monostearate and/or peregal A-20.
Described Antrodia Camphorata extract adopts following methods preparation
Antrodia Camphorata sporophore is adopted water and alcohol steep respectively, obtains flooding extract and alcohol steep thing by after water extract and alcohol extract respectively drying, and both are merged as Antrodia Camphorata extract.
Described Antrodia Camphorata extract preparation technology be
1) after the Antrodia Camphorata sporophore of drying being mixed according to the ratio of w/v 1:10 ~ 20 with water at 70 ~ 95 DEG C lixiviate 30-60min, and extracting solution concentrate drying is obtained flooding extract,
2) by step 1) in Antrodia Camphorata sporophore after flooding, add and be equivalent to raw material Antrodia Camphorata sporophore weight 5-8 ethanol doubly, be heated to backflow lixiviate 60 ~ 90nin, filter and obtain filtrate,
3) will through step 2) in the Antrodia Camphorata sporophore of alcohol steep, then add and be equivalent to raw material Antrodia Camphorata sporophore weight 5-8 ethanol doubly, be heated to backflow lixiviate 60 ~ 90nin, filter and obtain filtrate
4) by step 2), 3) filtrate that obtains merges concentrate drying and obtains alcohol steep thing, merge alcohol steep thing and flooding extract as Antrodia Camphorata extract.
Antrodia Camphorata extract emulsifiable paste provided by the invention, adopts following methods preparation
1) preparation of aqueous phase, after first soybean phospholipid being dissolved in ethanol, add the Antrodia Camphorata extract of recipe quantity, after abundant mixing, spraying dry obtains the composite micro-powder of Antrodia Camphorata extract and soybean phospholipid, composite micro-powder, wetting agent, antioxidant, pH buffer agent are added in the water of recipe quantity and is heated to 60-90 DEG C and dissolves dispersion, obtain aqueous phase.
2) preparation of oil phase, the component as oil-phase component of getting recipe quantity is heated to melt (60 DEG C ~ 90 DEG C), adds antibiotic antiseptic and is stirred to evenly and get final product;
3) preparation of Antrodia Camphorata extract emulsifiable paste
By heated aqueous to after 60 DEG C ~ 90 DEG C, then the oil phase being heated to 60 ~ 90 DEG C is slowly joined in aqueous phase, stir while adding and evenly namely obtain Antrodia Camphorata extract emulsifiable paste to condensation.
Described in the present invention, percentage ratio is all weight percentage.
We find under study for action, soybean phospholipid and Antrodia Camphorata compound extract is adopted to be the emulsifiable paste of active component, the phototoxicity of remarkable reduction Antrodia Camphorata extract used time outside, and the safety in utilization of Antrodia Camphorata extract emulsifiable paste can be improved, overcome Antrodia Camphorata extract outside the used time due to its phototoxicity bring to use restriction, the ability of its anti-skin photoage can be improved again simultaneously, sun-proof or sun-proof secondary purpose can be applied.Find only have soybean phospholipid can realize this effect additionally by contrast, use merely Antrodia Camphorata extract or jointly use then without this effect with conventional other phospholipid such as Ovum Gallus domesticus Flavus lecithin.
Detailed description of the invention
Below in conjunction with embodiment, the specific embodiment of the present invention is described in further detail.Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
The preparation of Antrodia Camphorata extract
Described Antrodia Camphorata extract preparation technology be
1) after the Antrodia Camphorata sporophore of drying being mixed according to the ratio of w/v 1:15 with water at 80 DEG C lixiviate 45min, and extracting solution concentrate drying is obtained flooding extract,
2) by step 1) in Antrodia Camphorata sporophore after flooding, add the ethanol being equivalent to raw material Antrodia Camphorata sporophore weight 7 times, be heated to backflow lixiviate 60nin, filter and obtain filtrate,
3) will through step 2) in the Antrodia Camphorata sporophore of alcohol steep, then add the ethanol being equivalent to raw material Antrodia Camphorata sporophore weight 7 times, be heated to backflow lixiviate 60nin, filter and obtain filtrate
By step 2), 3) filtrate that obtains merges concentrate drying and obtains alcohol steep thing, merge alcohol steep thing and flooding extract as Antrodia Camphorata extract.
The preparation of Antrodia Camphorata extract emulsifiable paste comprises the following steps (water adopted during preparation is purified water)
1) preparation of aqueous phase, after first soybean phospholipid being dissolved in ethanol, adds the Antrodia Camphorata extract of recipe quantity, and fully after mixing, spraying dry obtains the composite micro-powder of Antrodia Camphorata extract and soybean phospholipid.
Composite micro-powder, wetting agent, antioxidant are added in the water of recipe quantity and is heated to 60-90 DEG C and dissolves dispersion, add pH buffer agent and regulate pH to 6.5-7.0 to obtain aqueous phase.
2) preparation of oil phase,
The component as oil-phase component of getting recipe quantity is heated to melt (60 DEG C ~ 90 DEG C), adds antibiotic antiseptic and is stirred to evenly and get final product;
3) preparation of Antrodia Camphorata extract emulsifiable paste
By heated aqueous to after 60 DEG C ~ 90 DEG C, then the oil phase being heated to 60 ~ 90 DEG C is slowly joined in aqueous phase, stir while adding and evenly namely obtain Antrodia Camphorata extract emulsifiable paste to condensation.
In each embodiment of the present invention, emulsifiable paste amount of preparation is 1000g, and pH buffer agent is phosphoric acid/phosphate buffer, and antibiotic antiseptic is methyl hydroxybenzoate, and antioxidant all adopts EDTA-CaNa
2.
The composition of the soybean phospholipid (soybean lecithin) used in the specific embodiment of the invention is: phosphatidylcholine PC (lecithin) 25-32%, PHOSPHATIDYL ETHANOLAMINE PE (cephalin) 15-22%, phosphatidylinositols PI (lipositol) about 15%, phosphatidyl glycerol PG (sphingomyelin) about 16%, phosphatidic acid about PA4%, other phospholipid about 8%
Ovum Gallus domesticus Flavus lecithin (Egg Yolk Lecithin, CAS:93685-90-6) meets the standard of Chinese Pharmacopoeia 2010 editions.
The concrete formula of embodiment 1 ~ 6 sees the following form (unit/g, emulsifiable paste amount of preparation is 1000g, and surplus is purified water)
Pharmacological Examples 1
1 material
1.1 organize hydroxyproline test kit, build up Bioengineering Research Institute purchased from Nanjing.
1.2 animal Kunming mouses, female Mus, body weight 20-25g, 7-8 week age, provided by Shanghai Univ. of Traditional Chinese Medicine's Experimental Animal Center, often organize 10, grouping with administration as following table:
Remarks: in experimental group 5,6, replace composite micro-powder for the preparation of aqueous phase after Antrodia Camphorata extract is added ethanol, directly add soybean phospholipid powder when preparing aqueous phase in embodiment 7.Antrodia Camphorata extract and soybean phospholipid is not added during the substrate preparation that normal group and model group use.
2 methods
Mouse back skin is shaved only by 2.1 animal groupings and model preparation electric hair clipper, about 2cm × 2cm, baring skin, random point 3 groups.Normal group, skin smears embodiment 3 emulsifiable paste matrix, without other any process every day; 2nd group is model group, and skin smears 1%8-methoxypsoralen (8-MOP) 1 time every day, after 1.5-2h, gives 190 μ w/cm
2uVA irradiate, cumulative exposure 138h (integrated intensity 95J/cm
2), irradiate and terminate bark fetching skin in rear 24h; It is Experimental agents that each test group of animals skin smears concentration every day, repastes and smears 1%8-MOP, after 1.5-2h, give 190 μ w/cm after slightly dry
2uVA irradiate, cumulative exposure 138h (integrated intensity 95J/cm
2), irradiate and terminate bark fetching skin in rear 24h; To obtaining skin histology, carry out hydroxyproline content mensuration, and according to the form below carries out phototoxicity scoring to laboratory animal
2.2 organize hydroxyproline content to measure assay method operates according to test kit, detects histone simultaneously, calculates hydroxyproline/total protein ratio, t check analysis result.Result is as following table:
Testing result as following table (
n=10)
In prior art, the content of hydroxyproline is often used to characterize the degree of aging of skin, experimental result shows, the experimental group 1-2 of the emulsifiable paste adopting the embodiment of the present invention to prepare, hydroxyproline in the skin of laboratory animal/total protein ratio higher and closer to the normal group as negative control, and as the model group of positive control, hydroxyproline/total protein ratio occurs obviously to decline.Illustrate that Antrodia Camphorata extract emulsifiable paste provided by the invention significantly can suppress the photoaging of skin, and phototoxicity scoring shows that the animal of experimental group 1-2 average score compared with model group increases not obvious, and do not adopt the experimental group 3-6 of soybean phospholipid and Antrodia Camphorata compound extract, although hydroxyproline/total protein scale increases compared with model group, but phototoxicity scoring shows that its medicine used all shows stronger phototoxicity under ultraviolet radiation, to illustrate under the condition that illumination is strong and inapplicable, and experimental group 7 shows, simple use soybean phospholipid, hydroxyproline/total protein ratio and phototoxicity two indexs of marking all are had no significant effect.
Claims (5)
1. an Antrodia Camphorata emulsifiable paste, is characterized in that described Antrodia Camphorata emulsifiable paste is made up of following component
Antrodia Camphorata extract 0.5 ~ 2%; Soybean phospholipid, the weight ratio of soybean phospholipid and Antrodia Camphorata extract is 2-3:1;
Antioxidant 0.1% ~ 0.3%, remains on the pH buffer agent of 5 ~ 7.5, antibiotic antiseptic 0.05% ~ 0.1%, wetting agent 3%-10%, oil-phase component 20%-30% by pH value, and the water of surplus;
Described antioxidant is selected from EDTA-2Na, EDTA-CaNa
2, one or more in tocopherol;
Described antibiotic antiseptic is selected from one or more in methyl hydroxybenzoate, ethyl hydroxybenzoate, propylparaben.
Described pH buffer agent is selected from phosphoric acid/phosphate buffer, acetic acid/acetate buffer, citric acid/citrate buffer agent,
Described wetting agent is selected from one or more in glycerol, propylene glycol, sorbitol,
Described oil-phase component by as the hexadecanol of higher alcohol and/or octadecanol, as the vaseline of consistency modifiers and/or liquid paraffin and emulsifying agent composition; Described emulsifying agent is selected from glyceryl monostearate and/or peregal A-20;
Described Antrodia Camphorata extract adopts following methods preparation:
Antrodia Camphorata sporophore is adopted water and alcohol steep respectively, obtains flooding extract and alcohol steep thing by after water extract and alcohol extract respectively drying, and both are merged as Antrodia Camphorata extract.
2. Antrodia Camphorata emulsifiable paste as claimed in claim 1, is characterized in that described antioxidant is EDTA-CaNa
2.
3. Antrodia Camphorata emulsifiable paste as claimed in claim 1, is characterized in that described pH buffer agent is phosphoric acid/phosphate buffer.
4. Antrodia Camphorata emulsifiable paste as claimed in claim 1, is characterized in that described wetting agent is selected from glycerol.
5. Antrodia Camphorata emulsifiable paste as described in as arbitrary in Claims 1 to 4, is characterized in that the preparation technology of described Antrodia Camphorata extract is
1) after the Antrodia Camphorata sporophore of drying being mixed according to the ratio of w/v 1:10 ~ 20 with water at 70 ~ 95 DEG C lixiviate 30-60min, and extracting solution concentrate drying is obtained flooding extract,
2) by step 1) in Antrodia Camphorata sporophore after flooding, add and be equivalent to Antrodia Camphorata sporophore weight 5-8 ethanol doubly, be heated to backflow lixiviate 60 ~ 90nin, filter and obtain filtrate,
3) will through step 2) in the Antrodia Camphorata sporophore of alcohol steep, then add and be equivalent to Antrodia Camphorata sporophore weight 5-8 ethanol doubly, be heated to backflow lixiviate 60 ~ 90nin, filter and obtain filtrate
4) by step 2), 3) filtrate that obtains merges concentrate drying and obtains alcohol steep thing, merge alcohol steep thing and flooding extract as Antrodia Camphorata extract.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510432169.9A CN104958249B (en) | 2015-07-21 | 2015-07-21 | Antrodia camphorata emulsifiable paste |
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| CN201510432169.9A CN104958249B (en) | 2015-07-21 | 2015-07-21 | Antrodia camphorata emulsifiable paste |
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| CN104958249B CN104958249B (en) | 2018-01-09 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110177113A1 (en) * | 2010-01-19 | 2011-07-21 | Chieh-Chou Yu | Use of antrodia camphorata for treating diseases |
| CN103372169A (en) * | 2012-04-13 | 2013-10-30 | 张立中 | Preparation for treating alcoholism and dispelling effects of alcohol |
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2015
- 2015-07-21 CN CN201510432169.9A patent/CN104958249B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110177113A1 (en) * | 2010-01-19 | 2011-07-21 | Chieh-Chou Yu | Use of antrodia camphorata for treating diseases |
| CN102151291A (en) * | 2010-01-19 | 2011-08-17 | 游介宙 | Use of antrodia camphorata for treating diseases |
| CN103372169A (en) * | 2012-04-13 | 2013-10-30 | 张立中 | Preparation for treating alcoholism and dispelling effects of alcohol |
Non-Patent Citations (1)
| Title |
|---|
| 穆筱梅等: "脂质体用大豆卵磷脂的制备及质量评价", 《大豆科学》 * |
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Effective date of registration: 20190927 Address after: Room 1201, Wutai Dongyuan, 36 Shanghai Road, Gulou District, Nanjing City, Jiangsu Province Patentee after: Jiang Ai Liang Address before: 300385 Tianjin city Xiqing District temple town Qing Ning Hou Cunjin green farm 500 meters West (Tianjin Kusun freshwater aquaculture Co. Hospital No. 8 workshop) Patentee before: Zhisheng (Tianjin) Biotechnology Co., Ltd. |
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Granted publication date: 20180109 Termination date: 20200721 |