Antrodia camphorata emulsifiable paste
Technical field
The present invention relates to a kind of Antrodia camphorata extract emulsifiable paste.
Background technology
Antrodia camphorata, referred to as " antrodia ", scientific name is Antrodia cinnamomea, is that one kind is grown in the cinnamomum kanehirai of Taiwan
Medicinal fungi.Antrodia camphorata has removing toxic substances liver protection, anticancer, reinforced immunological, antiallergy, reducing blood lipid and other effects, can be used as health care
Product are taken, and compared with ganoderma lucidum, the triterpene content of Antrodia camphorata fructification is higher, have stronger health-care effect, are extracted in Antrodia camphorata
The beyond the region of objective existence used time can play a part of improve skin quality, but under study for action find be used as Antrodia camphorata extract in as chief active into
The triterpenes divided has certain phototoxicity, limits its application in externally applied product, therefore provides one kind and overcome its light malicious
Property Antrodia camphorata externally applied product turn into need to solve the problems, such as in the prior art.
The content of the invention
In order to solve the above technical problems, it is an object of the invention to provide a kind of Antrodia camphorata emulsifiable paste, under study for action we have found that
Use soybean lecithin to coordinate with Antrodia camphorata extract with certain proportion, can significantly reduce Antrodia camphorata extract in the outer used time
Phototoxicity, and a kind of Antrodia camphorata emulsifiable paste is provided based on this discovery.
The invention provides a kind of Antrodia camphorata emulsifiable paste, described Antrodia camphorata emulsifiable paste is made up of following components
Antrodia camphorata extract 0.5%~2%;Soybean lecithin, the weight ratio of soybean lecithin and Antrodia camphorata extract is 2-3:
1;
Antioxidant 0.1%~0.3%, pH value is maintained at 5~7.5 pH buffer, and antibiotic antiseptic 0.05%~
0.1%, NMF 3%-10%, oil-phase component 20%-30%, and the water of surplus;
The antioxidant is selected from EDTA-2Na, EDTA-CaNa2, one or more in tocopherol;
It is preferred that EDTA-CaNa2。
One or more of the antibiotic antiseptic in methyl hydroxybenzoate, ethylparaben, propylben.
The pH buffer is selected from phosphoric acid/phosphate buffer, acetic acid/acetate buffer, citric acid/citrate and delayed
Electuary, preferably phosphoric acid/phosphate buffer
One or more of the NMF in glycerine, propane diols, sorbierite, preferably glycerine.
The oil-phase component by the hexadecanol and/or octadecyl alcolol as higher alcohol, as consistency modifiers vaseline and/
Or atoleine and emulsifying agent composition;The emulsifying agent is selected from glycerin monostearate and/or peregal A-20.
The Antrodia camphorata extract is prepared using following methods
Water and alcohol steep is respectively adopted in Antrodia camphorata fructification, after water extract and alcohol extract are separately dried
Antrodia camphorata extract is incorporated as to flooding extract and alcohol steep thing, and by both.
The preparation technology of the Antrodia camphorata extract is
1) by dry Antrodia camphorata fructification and water according to w/v 1:70~95 after 10~20 ratio mixing
30-60min is extracted at DEG C, and extract solution is concentrated and dried to obtain flooding extract,
2) by the Antrodia camphorata fructification in step 1) after flooding, add equivalent to raw material Antrodia camphorata fructification weight
5-8 times of ethanol, backflow 60~90min of extraction is heated to, is filtrated to get filtrate,
3) the Antrodia camphorata fructification of alcohol steep in step 2) will be passed through, added equivalent to raw material Antrodia camphorata fructification weight
The ethanol of 5-8 times of amount, backflow 60~90min of extraction is heated to, is filtrated to get filtrate
4) filtrate obtained by step 2), 3) merges concentrate drying and obtains alcohol steep thing, by alcohol steep thing and water logging
Extract is incorporated as Antrodia camphorata extract.
Antrodia camphorata extract emulsifiable paste provided by the invention, is prepared using following methods
1) preparation of aqueous phase, after soybean lecithin first is dissolved in into ethanol, the Antrodia camphorata extract of recipe quantity is added, is sufficiently mixed
Spray drying obtains the composite micro-powder of Antrodia camphorata extract and soybean lecithin afterwards, by composite micro-powder, NMF, antioxidant, pH
It is scattered that 60-90 DEG C of dissolving is heated in the water of buffer addition recipe quantity, obtains aqueous phase.
2) preparation of oil phase, take the component as oil-phase component of recipe quantity to be heated to melting (60 DEG C~90 DEG C), add
Antibiotic antiseptic, which is stirred until homogeneous, to be produced;
3) preparation of Antrodia camphorata extract emulsifiable paste
By heated aqueous to after 60 DEG C~90 DEG C, then it would be heated to 60~90 DEG C of oil phase and be slowly added into aqueous phase, side
Addition side, which is evenly stirred until to condense, produces Antrodia camphorata extract emulsifiable paste.
Heretofore described percentage is weight percentage.
Under study for action it was found that using emulsifiable paste of the soybean lecithin with Antrodia camphorata compound extract for active component, significantly
Phototoxicity of the Antrodia camphorata extract in the outer used time is reduced, and the safety in utilization of Antrodia camphorata extract emulsifiable paste can be improved, is overcome
The limitation to using that Antrodia camphorata extract is brought in the outer used time due to its phototoxicity, that and can improves its anti-skin light simultaneously is old
The ability of change, sun-proof or sun-proof secondary purpose can be applied.Find that only soybean lecithin can be realized additionally by contrast
This effect, imitated using Antrodia camphorata extract or with other conventional phosphatide if egg yolk lecithin is used in conjunction with without this merely
Fruit.
Embodiment
With reference to embodiment, the embodiment of the present invention is described in further detail.Following examples are used for
Illustrate the present invention, but be not limited to the scope of the present invention.
The preparation of Antrodia camphorata extract
The preparation technology of the Antrodia camphorata extract is
1) by dry Antrodia camphorata fructification and water according to w/v 1:Extracted after 15 ratio mixing at 80 DEG C
45min, and extract solution is concentrated and dried to obtain flooding extract,
2) by the Antrodia camphorata fructification in step 1) after flooding, add equivalent to raw material Antrodia camphorata fructification weight
7 times of ethanol, backflow extraction 60min is heated to, is filtrated to get filtrate,
3) the Antrodia camphorata fructification of alcohol steep in step 2) will be passed through, added equivalent to raw material Antrodia camphorata fructification weight
The ethanol of 7 times of amount, backflow extraction 60min is heated to, is filtrated to get filtrate
By step 2), 3) filtrate obtained merges concentrate drying and obtains alcohol steep thing, by alcohol steep thing and flooding
Thing is incorporated as Antrodia camphorata extract.
The preparation of Antrodia camphorata extract emulsifiable paste comprises the following steps (water used during preparation is purified water)
1) preparation of aqueous phase, after soybean lecithin first is dissolved in into ethanol, the Antrodia camphorata extract of recipe quantity is added, is sufficiently mixed
Spray drying obtains the composite micro-powder of Antrodia camphorata extract and soybean lecithin afterwards.
Composite micro-powder, NMF, antioxidant add in the water of recipe quantity to be heated to 60-90 DEG C of dissolving scattered, adds
PH buffer regulation pH to 6.5-7.0 obtains aqueous phase.
2) preparation of oil phase,
Take the component as oil-phase component of recipe quantity to be heated to melting (60 DEG C~90 DEG C), add antibiotic antiseptic stirring
To uniformly producing;
3) preparation of Antrodia camphorata extract emulsifiable paste
By heated aqueous to after 60 DEG C~90 DEG C, then it would be heated to 60~90 DEG C of oil phase and be slowly added into aqueous phase, side
Addition side, which is evenly stirred until to condense, produces Antrodia camphorata extract emulsifiable paste.
Emulsifiable paste amount of preparation is 1000g in each embodiment of the invention, and pH buffer is phosphoric acid/phosphate buffer, is resisted
Bacterium preservative is methyl hydroxybenzoate, and antioxidant uses EDTA-CaNa2。
The composition of the soybean lecithin (soybean lecithin) used in the specific embodiment of the invention is:Phosphatidyl courage
Alkali PC (lecithin) 25-32%, phosphatidyl-ethanolamine PE (cephalin) 15-22%, phosphatidylinositols PI (lipositol) 15%
Left and right, phosphatidyl glycerol PG (sphingomyelin) 16% or so, phosphatidic acid PA4% or so, other phosphatide 8% or so
Egg yolk lecithin (Egg Yolk Lecithin, CAS:93685-90-6) meet the standard of Chinese Pharmacopoeia 2010 editions.
The specific formula of embodiment 1~6 see the table below (unit/g, emulsifiable paste amount of preparation are 1000g, and surplus is purified water)
Pharmacological Examples 1
1 material
1.1 tissue hydroxyproline kits, Bioengineering Research Institute is built up purchased from Nanjing.
1.2 animal Kunming mouses, female mice, body weight 20-25g, 7-8 week old, by Shanghai Univ. of Traditional Chinese Medicine experimental animal
The heart provides, every group 10, packet and administration such as following table:
Remarks:In experimental group 5,6, Antrodia camphorata extract is added after ethanol and replaces composite micro-powder to be used to prepare aqueous phase i.e.
Can, it is directly added into soybean lecithin powder when aqueous phase is prepared in embodiment 7.It is not added with during the substrate preparation that normal group uses with model group
Enter Antrodia camphorata extract and soybean lecithin.
2 methods
2.1 animal packets and model preparation are shaved mouse back skin only with electric hair clipper, about 2cm × 2cm, exposed skin
Skin, random point 3 groups.Normal group, skin smears an emulsifiable paste matrix of embodiment 3 daily, without other any processing;2nd group is mould
Type group, skin smear 1%8- Medadinines (8-MOP) 1 time daily, after 1.5-2h, give 190 μ w/cm2UVA irradiation,
Cumulative exposure 138h (integrated intensity 95J/cm2), irradiation terminates to take skin in rear 24h;Each test group of animals skin is smeared daily
Concentration is Experimental agents, smears 1%8-MOP after slightly dry again, after 1.5-2h, gives 190 μ w/cm2UVA irradiation, cumulative exposure
138h (integrated intensity 95J/cm2), irradiation terminates to take skin in rear 24h;To obtaining skin histology, hydroxyproline content survey is carried out
It is fixed, and according to the form below carries out phototoxicity scoring to experimental animal
2.2 tissue hydroxyproline content measure assay methods operate according to kit, while detect histone, calculate hydroxyl
Proline/total protein ratio, t check analysis results.As a result such as following table:
Testing result such as following table (N=10)
In the prior art, the content of hydroxyproline is usually utilized to characterize the degree of aging of skin, test result indicates that, adopt
The experimental group 1-2 of the emulsifiable paste prepared with the embodiment of the present invention, in the skin of experimental animal hydroxyproline/total protein ratio it is higher and
Closer to the normal group as negative control, and be used as the model group of positive control, the generation of hydroxyproline/total protein ratio is bright
It is aobvious to decline.Illustrate that Antrodia camphorata extract emulsifiable paste provided by the invention can significantly inhibit the light aging of skin, and phototoxicity scores
Show that the experimental group 1-2 animal average score compared with model group increases unobvious, and do not carried using soybean lecithin with Antrodia camphorata
The experimental group 3-6 of thing compound is taken, although hydroxyproline/total protein scale increases compared with model group, phototoxicity
Scoring shows that its medicine used all shows stronger phototoxicity under ultraviolet irradiation, illustrates under conditions of illumination is strong
And do not apply to, and experimental group 7 shows, merely using soybean lecithin, hydroxyproline/total protein ratio and phototoxicity are scored two
Index has no significant effect.