CN104957726A - Application of gardenia series pigments to bacteriostasis - Google Patents

Application of gardenia series pigments to bacteriostasis Download PDF

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Publication number
CN104957726A
CN104957726A CN201510397093.0A CN201510397093A CN104957726A CN 104957726 A CN104957726 A CN 104957726A CN 201510397093 A CN201510397093 A CN 201510397093A CN 104957726 A CN104957726 A CN 104957726A
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gardenia
pigment
application
yellow
bacteriostasis
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CN201510397093.0A
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CN104957726B (en
Inventor
方尚玲
陈茂彬
曹敬华
严锦
孟鑫
周敬波
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Shanghai Ruishenglian Information Technology Co ltd
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Hubei University of Technology
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses an application of gardenia series pigments to bacteriostasis, which belongs to the field of food additives. According to the application, bacteriostasis experiments show that gardenia yellow pigments, gardenia red pigments and gardenia blue pigments can inhibit the growth of gram-positive bacteria and gram-negative bacteria, such as bacillus subtilis and proteus. On the basis of the function of bacteriostasis, the natural gardenia series pigments with three color primaries of red, yellow and green are food additives capable of adjusting any color, have the effects of bacteriostasis and corrosion prevention, can be used for coloring and prolonging the expiration date of food, can be used for preparing bacteriostatic agents or preservatives and have good application prospects.

Description

The application of cape jasmine series pigment in antibacterial
Technical field
The invention belongs to food additives field, be specifically related to the application of cape jasmine series pigment in antibacterial.
Background technology
At present at food-processing industry, the use of synthetic food color is very general.Synthetic food color refers to the organic pigment obtained by artificial chemical synthesis process, mainly with the aniline dyes separated from coal tar for raw material is made, but along with deepening continuously of toxicology and biological study, it is found that most of synthetic food color all can not provide nutriment to human body, what have even has comparatively serious toxicity and teratogenesis, carcinogenicity to human body.Many edible synthesized coloring matters are except itself or its metabolin are poisonous, also likely be mixed into the heavy metal such as arsenic and lead in process of production, due to heavy metal element stable in properties, not easily decomposed by human body or drain, easily precipitate in vivo, long term accumulation can cause the generation of the diseases such as subcutaneous sarcoma, liver and malignant lymphoma.
Natural food colour have safe and reliable, have no side effect, tone nature, close to advantages such as natural materials colors, improving constantly in recent years along with the growing material life demand of people and scientific level, utilize nontoxic natural materials to extract edible, cosmetics and medical pigment, become a kind of new development trend.But at present, in the use procedure of natural colouring matter, mainly with being colored as main purpose, this makes the range of application of some natural colouring matters be limited.
Summary of the invention
The object of the invention is to the range of application expanding cape jasmine series pigment, the application of cape jasmine series pigment in antibacterial is provided.
Object of the present invention is achieved through the following technical solutions:
By bacteriostatic experiment, the present invention finds that cape jasmine series pigment can suppress the growth of gram-positive bacteria, Gram-negative bacteria, show that cape jasmine series pigment has antibacterial effect.Based on this, cape jasmine series pigment can be used for antibacterial in.Described cape jasmine series pigment comprises Gardenia Yellow, gardenia red pigment and gardenia blue pigment three kinds of pigments, and these three kinds of pigments all can suppress bacillus subtilis (Bacillus subtilis), proteus (Proteusbacillus vulgaris) growth.
Gardenia Yellow, gardenia red pigment and gardenia blue pigment are as the natural gardenia series pigment possessing Huang, red, blue three kinds of primary colours, be the food additives of adjustable random color and have antibacterial antisepsis concurrently, can be used for the painted of the numerous food such as fruit drink, pre-rectification, dairy products, cake, snacks and extend effective period of food quality.
Therefore, cape jasmine series pigment may be used for preparing bacteriostatic agent or anticorrisive agent.
A kind of bacteriostatic agent or anticorrisive agent, comprise in Gardenia Yellow, gardenia red pigment and gardenia blue pigment one or more, the additive that other food allows can also be comprised.
The present invention has the following advantages and effect relative to prior art tool:
(1) cape jasmine series pigment is natural colouring matter.The Gardenoside of Gardenia Yellow and conversion gardenia red pigment and gardenia blue pigment all extracts from plant tissue, three kinds of pigments are natural colouring matter, there is the feature of safety non-toxic, can extensively make an addition in the middle of numerous food, effectively evade the murder by poisoning problem of adding synthetic dyestuff and may bring, improve the safety coefficient of food processing and manufacturing.
(2) cape jasmine series pigment has good bacteria resistance function.Cape jasmine series pigment can better imitate the color of natural goods, the soft harmony of painted tone.Bacteriostatic experiment proves, Gardenia Yellow, gardenia red and gardenia blue three kinds of pigments can grow by suppression harmful microbe in various degree, have had the effect of anticorrisive agent concurrently.
(3) cape jasmine series pigment contains physiologically active ingredient.Cape jasmine series pigment itself is a kind of nutrient, has certain pharmacological action.
Detailed description of the invention
Below in conjunction with embodiment, further detailed description is done to the present invention, but embodiments of the present invention are not limited thereto.
The preparation of embodiment 1 cape jasmine series pigment
(1) preparation of Gardenia Yellow
Cape jasmine fruit was pulverized 40 mesh sieves, and gained Cape Jasmine Fruit deionized water obtains Gardenia Yellow crude extract by feed liquid mass ratio 1:10 lixiviate 1h at 45 DEG C.
Refining of Gardenia Yellow: with AB-8 type macroporous absorbent resin absorption Gardenia Yellow, 30g wet resin is added in the triangular flask containing 100mL Gardenia Yellow crude extract, put into shaking table and adsorb more than 10h, then the resin in triangular flask is filtered together with solution, after collecting resin, fill post.Use 4BV (it is exactly 1BV that the floor space 2.0096 square centimeters of splitter is multiplied by the height of resin in splitter 14.5 centimetres) water, 5BV 20% ethanol, 6.5BV 60% alcohol flushing collect each flushing liquor respectively, mainly Gardenoside is contained, containing Gardenia Yellow in 60% alcohol flushing liquid in 4BV water and 5BV 20% alcohol flushing liquid.Obtain the highly purified Gardenia Yellow aqueous solution by after 60% alcohol flushing liquid, 60 DEG C of rotary evaporations concentrated removing ethanol, namely spraying dry obtains Gardenia Yellow powder.
(2) preparation of gardenia red pigment
The pretreatment of Gardenoside: in refining Gardenia Yellow process, collects 4BV water and 5BV 20% alcohol flushing liquid and merges, amalgamation liquid 70 DEG C of rotary evaporations are concentrated the Gardenoside aqueous solution that 10 times obtain high concentration.In the Gardenoside aqueous solution after concentrated, add the NaOH accounting for Gardenoside aqueous solution quality 10%, 50 DEG C of magnetic agitation 4h, then add the citric acid accounting for Gardenoside aqueous solution quality 25%, be stirred to citric acid and dissolve completely, 4 DEG C of refrigerator cold-storages are for subsequent use.
At solid medium, (formula is Mucor: 16g wheat bran, 24mL nutrient solution.Nutrient solution: (NH 4) 2sO 41%, KH 2pO 40.4%, MgSO 47H 2o 0.2%, surplus is water.) middle cultivation 5d, fermentation solid matrix deionized water is extracted 2h by feed liquid mass ratio 1:10 in 45 DEG C of water-baths, the centrifugal 10min of extract 8000rpm, collect supernatant and obtain beta-glucosidase crude enzyme liquid.
In beta-glucosidase crude enzyme liquid, pretreated Gardenoside and sodium glutamate is added according to the ratio of the pretreated Gardenoside of 50mL beta-glucosidase crude enzyme liquid interpolation 20mL, 5g sodium glutamate, 50 DEG C of vacuum heat-preserving 24h, the boiling water bath enzyme that goes out is lived 2h, the obtained thick solution of gardenia red pigment.
With D-1300 macroreticular resin separation and purification gardenia red pigment, detailed process is: added to by 30g wet resin in the triangular flask containing the thick solution of 100mL gardenia red pigment, put into shaking table and adsorb more than 10h, then the resin in triangular flask is filtered together with solution, collect resin and then fill post.Remove impurity with 1BV deionized water, 2BV 15% alcohol flushing, then use 2BV55% alcohol flushing respectively, collect 2BV 55% alcohol flushing liquid.Obtain the highly purified gardenia red pigment aqueous solution by after 2BV 55% alcohol flushing liquid 60 DEG C of rotary evaporations concentrated removing ethanol, namely spraying dry obtains gardenia red pigment powder.
(3) preparation of gardenia blue pigment
In refining Gardenia Yellow process, collect 4BV water and 5BV 20% alcohol flushing liquid and merge, amalgamation liquid 70 DEG C of rotary evaporations are concentrated the Gardenoside aqueous solution that 7 times obtain high concentration, for subsequent use.
The preparation of beta-glucosidase crude enzyme liquid is with (2).
In beta-glucosidase crude enzyme liquid, the Gardenoside aqueous solution and sodium glutamate is added according to the ratio of the 50mL beta-glucosidase crude enzyme liquid interpolation 15mL Gardenoside aqueous solution, 5g sodium glutamate, 50 DEG C of insulation 24h, the boiling water bath enzyme that goes out is lived 30min, the obtained thick solution of gardenia blue pigment.
With X-5 resin separation purification gardenia blue pigment, detailed process is: added in the triangular flask containing the thick solution of 100mL gardenia blue pigment by 30g wet resin, puts into shaking table and adsorbs more than 10h, then filtered together with solution by the resin in triangular flask, collect resin, then fill post.Remove impurity with 2BV deionized water, 1BV 10% alcohol flushing, then use 2BV 60% alcohol flushing respectively, collect 2BV 60% alcohol flushing liquid.Obtain the highly purified gardenia blue pigment aqueous solution by after 2BV 60% alcohol flushing liquid 60 DEG C of rotary evaporations concentrated removing ethanol, namely spraying dry obtains gardenia blue pigment powder.
The bacteriostatic experiment of embodiment 2 cape jasmine series pigment
Operate under aseptic working platform, the beef-protein medium prepared is cooled to about 60 DEG C and is poured in the culture dish of sterilizing, drawing concentration with aseptic liquid-transfering gun is 10 6the bacillus subtilis of cfu/mL or the bacterium liquid 200 μ L of proteus, be coated with rod with sterile glass triangle and it coated planar surface equably, stand-by.Each flat board puts 6 aseptic Oxford cups (internal diameter of Oxford cup is 6mm, and external diameter is 7mm), and middle 1 aseptic Oxford cup is blank, and all the other 5 Oxford cups are sample sets.Blank adds 100 μ L sterilized waters, the concentration adding filtration sterilization in sample sets is the aqueous solution 100 μ L of the gardenia red pigment of 20 μ g/mL, Gardenia Yellow or gardenia blue pigment, all flat boards take out place 10h in 4 DEG C of refrigerators after, be placed in 37 DEG C of constant temperature culture 24h again, observe antibacterial situation, the diameter of 5 inhibition zones is measured, calculating mean value with ruler.The results are shown in Table 1.
The measurement result of table 1 antibacterial circle diameter
Bacterial classification Gardenia red pigment Gardenia Yellow Gardenia blue pigment
Bacillus subtilis 21.2mm 19.3mm 16.1mm
Proteus 21.6mm 17.4mm 13.6mm
Minimum inhibitory concentration (MIC) experiment of embodiment 3 cape jasmine series pigment
Operate under aseptic working platform, the beef-protein medium of the sterilizing prepared is cooled to about 60 DEG C and is poured in the culture dish of sterilizing, drawing concentration with aseptic liquid-transfering gun is 10 6the bacillus subtilis of cfu/mL or the bacterium liquid 200 μ L of proteus, be coated with rod with sterile glass triangle and it coated planar surface equably, stand-by.
Each flat board puts 6 aseptic Oxford cups, and (internal diameter of Oxford cup is 6mm, external diameter is 7mm), middle 1 aseptic Oxford cup is blank, all the other 5 Oxford cups are sample sets, blank adds 100 μ L sterilized waters, 5 Oxford cup numberings 1 in sample sets, 2, 3, 4, 5, the concentration adding 100 μ L filtration sterilizations is successively 20 μ g/mL, 15 μ g/mL, 10 μ g/mL, 5 μ g/mL, the gardenia red pigment of 1 μ g/mL, the aqueous solution of Gardenia Yellow or gardenia blue pigment, all flat boards are taken out after 10h placed by 4 DEG C of refrigerators, observed result after 37 DEG C of constant incubators cultivate 24h, according to the minimum inhibitory concentration (MIC) judging cape jasmine series pigment with or without inhibition zone.
The minimum inhibitory concentration of table 2 cape jasmine series pigment
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from Spirit Essence of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.

Claims (10)

1. the application of cape jasmine series pigment in antibacterial, is characterized in that: described subfamily pigment comprises Gardenia Yellow, gardenia red pigment and gardenia blue pigment.
2. application according to claim 1, is characterized in that: cape jasmine series pigment is suppressing the application in gram-positive bacteria, Gram-negative bacteria growing.
3. application according to claim 1, is characterized in that: Gardenia Yellow is suppressing the application in bacillus subtilis, proteus growth.
4. application according to claim 1, is characterized in that: gardenia red pigment is suppressing the application in bacillus subtilis, proteus growth.
5. application according to claim 1, is characterized in that: gardenia blue pigment is suppressing the application in bacillus subtilis, proteus growth.
6. Gardenia Yellow, gardenia red pigment and/or gardenia blue pigment are extending the interim application of food preserving.
7. Gardenia Yellow, gardenia red pigment and/or gardenia blue pigment are preparing the application in bacteriostatic agent.
8. Gardenia Yellow, gardenia red pigment and/or gardenia blue pigment are preparing the application in anticorrisive agent.
9. bacteriostatic agent or an anticorrisive agent, is characterized in that: comprise in Gardenia Yellow, gardenia red pigment and gardenia blue pigment one or more.
10. bacteriostatic agent according to claim 9 or anticorrisive agent, is characterized in that: also comprise the additive that food allows.
CN201510397093.0A 2015-07-08 2015-07-08 Application of the cape jasmine series pigment in antibacterial Active CN104957726B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109601815A (en) * 2018-12-05 2019-04-12 广西山云生化科技有限公司 A kind of preparation method of Gardenoside food antiseptic Perserving materials
CN110697985A (en) * 2019-10-24 2020-01-17 山丹县康源清真牛羊肉食品有限责任公司 Slaughterhouse effluent disposal system

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003089641A (en) * 2001-07-12 2003-03-28 Lion Corp Agent for suppressing obligate anaerobe and composition for suppressing obligate anaerobe
CN102613243A (en) * 2012-03-12 2012-08-01 宋信宇 Nanometer Ag antimicrobial and preparation method thereof
CN103919841A (en) * 2014-03-19 2014-07-16 河南大学 Method for prolonging shelf life of eucommia ulmoides seed oil soft capsules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003089641A (en) * 2001-07-12 2003-03-28 Lion Corp Agent for suppressing obligate anaerobe and composition for suppressing obligate anaerobe
CN102613243A (en) * 2012-03-12 2012-08-01 宋信宇 Nanometer Ag antimicrobial and preparation method thereof
CN103919841A (en) * 2014-03-19 2014-07-16 河南大学 Method for prolonging shelf life of eucommia ulmoides seed oil soft capsules

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HEE-OCK BOO等: "Extraction and characterization of some natural plant pigments", 《NDUSTRIAL CROPS AND PRODUCTS》 *
SUNG-JIN KIM等: "Dyeability of Chitosan-pretreated Cotton Fabrics with Gardenia-based Colorant Mixtures", 《TEXTILE SCIENCE AND ENGINEERING》 *
杨萌等: "食用天然黄色素的研究近况", 《中国调味品》 *
董智: "栀子红的制备与精制", 《中国食品添加剂》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109601815A (en) * 2018-12-05 2019-04-12 广西山云生化科技有限公司 A kind of preparation method of Gardenoside food antiseptic Perserving materials
CN109601815B (en) * 2018-12-05 2021-09-17 广西山云生化科技股份有限公司 Preparation method of geniposide food preservative and fresh-keeping material
CN110697985A (en) * 2019-10-24 2020-01-17 山丹县康源清真牛羊肉食品有限责任公司 Slaughterhouse effluent disposal system

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