CN104957359A - Method for producing food-grade antioxidative peptide by using corn protein powder - Google Patents
Method for producing food-grade antioxidative peptide by using corn protein powder Download PDFInfo
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- CN104957359A CN104957359A CN201510360979.8A CN201510360979A CN104957359A CN 104957359 A CN104957359 A CN 104957359A CN 201510360979 A CN201510360979 A CN 201510360979A CN 104957359 A CN104957359 A CN 104957359A
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Abstract
The invention discloses a method for producing food-grade antioxidative peptide by using corn protein powder. The method comprises the following steps: crushing corn protein powder of which the protein content is not smaller than 50wt%, and compounding the crushed corn protein powder into a corn protein powder solution of which the protein content is 5-8%; hydrolyzing starch in the corn protein powder by amylase, and performing saccharification by adopting biological enzyme; performing filtering and washing; collecting filter residues, and performing saponification so as to remove fat; performing filtering and washing again; collecting filter residues; performing hydrolysis under the condition that compound protease exists; and finally, performing centrifugal drying so as to obtain the food-grade antioxidative peptide. The method disclosed by the invention aims to produce the food-grade antioxidative peptide by using the corn protein powder. The food-grade antioxidative peptide prepared by the method is high in content, is rich in carotene, and can be used as health-care food for human bodies.
Description
Technical field
The present invention relates to food processing field, specifically a kind of corn protein powder produces the method for food-grade antioxidant peptide.
Background technology
Corn is one of third place in the world grande culture thing, and China accounts for 22% of its total output.Along with the growth in the living standard of people, recent two decades comes flourish in China with the starch sugar industry of maize raw material, forms specialization, maximization gradually even to this day.The present invention's raw material used is exactly become in the process of starch at corn processing, isolated albumen water (i.e. gluten water) from coarse starch milk, then through air supporting, press filtration, drying obtain the thick corn protein powder of one, because this albumen powder contains more starch, fat, ash content, at present can only as the protein additive use of feedstuff industry, its use value reduces greatly.
Summary of the invention
A kind of corn protein powder is the object of the present invention is to provide to produce the method for food-grade antioxidant peptide, the food-grade antioxidant peptide content that the method prepares is high, and be rich in carrotene, substantially do not have fat, protein content enriches, there is peptide matters high concentration, grain is low, UA advantage in acid condition, can to reduce blood fat simultaneously, increase cholesterol from the excretion of enteron aisle, can be used as body-care food.
Concrete technical scheme of the present invention is: a kind of corn protein powder produces the method for food-grade antioxidant peptide, described method is specially: be mixed with the corn protein powder solution that zein powder content is 5% ~ 8% after protein content being no less than the corn protein powder pulverizing of 50wt%, biology enzyme is adopted to carry out saccharification with the starch in amylorrhexis corn protein powder, after filtration after washing, collect filter residue and carry out saponification except degrease, again after filtration washing, collect filter residue, be hydrolyzed under compound protease existent condition, eventually pass centrifugal drying and obtain food-grade antioxidant peptide,
Wherein, the concrete grammar be hydrolyzed under compound protease existent condition is: containing filter residue 15 ~ 20g, pH6.0, temperature 40 ~ 50 DEG C, the consumption 0.2L/ ton corn protein powder of compound protease, 2 ~ 3 hours reaction time in often liter of hydrolyzate.
Produce in the method for food-grade antioxidant peptide above-mentioned with corn protein powder, described compound protease is the compound protease that Novozymes Company produces.
Produce in the method for food-grade antioxidant peptide with corn protein powder above-mentioned, the concrete grammar of saponification is: the filter residue after filtration washing is diluted with water to 150 ~ 200g/L, then adds Na
2cO
3, be adjusted to pH8 ~ 10, under the condition of 45 ~ 55 DEG C, react a period of time.
Produce in the method for food-grade antioxidant peptide with corn protein powder above-mentioned, the used time of saponification is 2 ~ 4h.
Produce in the method for food-grade antioxidant peptide above-mentioned with corn protein powder, the concrete grammar of the starch in amylorrhexis corn protein powder is: 105 DEG C ~ 110 DEG C, under the condition of pH5.6 ~ 6.0, with the starch in amylorrhexis corn protein powder, the chromogenic reaction depending on iodine liquid is colourless terminal of arriving.
Produce in the method for food-grade antioxidant peptide with corn protein powder above-mentioned, the starch used time in amylorrhexis corn protein powder is 90 ~ 120 minutes.
Produce in the method for food-grade antioxidant peptide above-mentioned with corn protein powder, described amylase is the High-temperature Liquefaction amylase that Jie Nengke bioengineering Co., Ltd produces, and the diastatic consumption of described High-temperature Liquefaction is 1L/ ton corn protein powder.
Produce in the method for food-grade antioxidant peptide above-mentioned with corn protein powder, described saccharification biology enzyme used is carbohydrase, the concrete grammar of described saccharification is: regulate pH to be 4.5 ~ 5.5 the solution through amylorrhexis, temperature 60 C, saccharificatinn period is 18 ~ 24h.Specifically, the consumption of the carbohydrase described in carbohydrase described in the beta amylase that described carbohydrase is produced for Jie Nengke Chinese biological Engineering Co., Ltd is 0.7 ~ 1.5L/ton corn protein powder.
Produce in the method for food-grade antioxidant peptide with corn protein powder above-mentioned, after saponification, the temperature of filtration washing is 75 DEG C.
Produce in the method for food-grade antioxidant peptide with corn protein powder above-mentioned, described corn protein powder comprises following component: protein >=50wt%; Starch≤18wt%; Fat≤8wt%; Fiber≤3wt%; Ash content≤4wt%; Water≤5wt%.
Compared with prior art, beneficial effect of the present invention is:
1, corn protein powder can effectively fully decompose by technical scheme of the present invention, and the protein peptides of active anti-oxidation peptide about obtaining containing 2-4 the amino acid of 45wt%;
2, the present invention adopts unique saponification process, by determining after great many of experiments to adopt Na
2cO
3can improve the content of carrotene, and can improve the pot-life of carrotene, within the time reaching 120 days, the degradation rate of carrotene is lower than 20%.
3, technical scheme technique of the present invention is simple, and productive rate is high, and anti-oxidation peptide content is high;
Detailed description of the invention
Below in conjunction with detailed description of the invention, technical scheme of the present invention is described in further detail, but does not form any limitation of the invention.
embodiment 1
Raw material: corn protein powder
Raw material components: protein 65.5wt%; Starch 16wt%; Lipase 37 wt%; Fiber 3wt%; Ash content 4wt%; Water 4.5wt%; Carrotene 325 μ g/g.
Step 1: cross 60 ~ 80 mesh sieves after being pulverized by 1kg corn protein powder;
Step 2: the preparation that adds water becomes the corn protein powder solution that corn protein powder content is 7wt%, and through cyclone desanding;
Step 3: at 105 DEG C ~ 110 DEG C, starch under the condition of pH5.8, in the High-temperature Liquefaction amylorrhexis corn protein powder produced with 1mL Jie Nengke bioengineering Co., Ltd, the chromogenic reaction depending on iodine liquid is colourless terminal of arriving, when sharing 108 minutes;
Step 4: adding food grade salt acid for adjusting pH is 5, temperature 60 C, add the carbohydrase (Jie Neng section (China) bioengineering Co., Ltd, beta amylase) of 1mL, saccharificatinn period is 20h, uses plate filter filtration washing, collects filter residue;
Step 5: filter residue is diluted with water to 180g/L, then the Na adding that concentration is 15wt%
2cO
3, be adjusted to pH8 ~ 10, stirring reaction 3 hours under the condition of 45 ~ 55 DEG C, make fat fully saponification;
Step 6: adopt plate filter filtration washing temperature to be 75 DEG C, collect filter residue and be about 450g, filter residue and water are mixed to get in often liter of hydrolyzate containing filter residue 150 ~ 200g, adding food grade salt acid for adjusting pH is 6.0, add the compound protease that 0.45ml Novozymes Company produces, control temperature is 45 DEG C, reacts 3 hours;
Step 7: adopt supercentrifuge centrifugal and drying obtains food-grade antioxidant peptide with 3300r/min.The weight of food-grade antioxidant peptide is 360g.
embodiment 2
Raw material: corn protein powder
Raw material components: protein 66wt%; Starch 18wt%; Fat 6wt%; Fiber 2wt%; Ash content 3wt%; Water 5wt%; Carrotene 325 μ g/g.
Step 1: cross 60 ~ 80 mesh sieves after being pulverized by 1kg corn protein powder;
Step 2: the preparation that adds water becomes the corn protein powder solution that corn protein powder content is 8wt%, and through cyclone desanding;
Step 3: at 105 DEG C ~ 110 DEG C, starch under the condition of pH6, in the High-temperature Liquefaction amylorrhexis corn protein powder produced with 1mL Jie Nengke bioengineering Co., Ltd, the chromogenic reaction depending on iodine liquid is colourless terminal of arriving, when sharing 95 minutes;
Step 4: regulate pH to be 5, temperature 60 C, add 0.7ml carbohydrase (Jie Neng section (China) bioengineering Co., Ltd, beta amylase), saccharificatinn period is 20h, uses plate filter filtration washing, collects filter residue; ;
Step 5: filter residue is diluted with water to 200g/L, then the Na adding that concentration is 15wt%
2cO
3, be adjusted to pH8 ~ 10, stirring reaction 2 hours under the condition of 45 ~ 55 DEG C, make fat fully saponification;
Step 6: adopt plate filter filtration washing wash temperature to be 75 DEG C, collects filter residue and is about 400g, filter residue and water is mixed to get in often liter of hydrolyzate containing filter residue 150 ~ 200g, pH is regulated to be 6.0, add the compound protease that 0.4ml Novozymes Company produces, control temperature is 40 DEG C, reacts 3 hours;
Step 7: adopt supercentrifuge centrifugal and drying obtains food-grade antioxidant peptide with 3300r/min.The weight of food-grade antioxidant peptide is 385g.
embodiment 3
Raw material: corn protein powder
Raw material components: protein 66wt%; Starch 15wt%; Fat 8wt%; Fiber 2wt%; Ash content 4wt%; Water 5wt%; Carrotene 325 μ g/g.
Step 1: cross 60 ~ 80 mesh sieves after being pulverized by 1kg corn protein powder;
Step 2: the preparation that adds water becomes the corn protein powder solution that corn protein powder content is 5wt%, and through cyclone desanding;
Step 3: at 105 DEG C ~ 110 DEG C, starch under the condition of pH5.6, in the High-temperature Liquefaction amylorrhexis corn protein powder produced with 1ml Jie Nengke bioengineering Co., Ltd, the chromogenic reaction depending on iodine liquid is colourless terminal of arriving, when sharing 118 minutes;
Step 4: regulate pH to be 5.5, temperature 60 C adds 1.5ml carbohydrase (Jie Neng section (China) bioengineering Co., Ltd, beta amylase), and saccharificatinn period is 24h, uses plate filter filtration washing, collects filter residue;
Step 5: filter residue is diluted with water to 150g/L, then the Na adding that concentration is 15wt%
2cO
3, be adjusted to pH8 ~ 10, stirring reaction 2 hours under the condition of 45 ~ 55 DEG C, make fat fully saponification;
Step 6: adopt plate filter filtration washing wash temperature to be 75 DEG C, collect filter residue 500g, is mixed to get filter residue and water in often liter of hydrolyzate containing filter residue 150 ~ 200g, pH is regulated to be 6.0, add the compound protease that 0.5ml Novozymes Company produces, control temperature is 40 DEG C, reacts 3 hours;
Step 7: adopt supercentrifuge centrifugal and drying obtains food-grade antioxidant peptide with 3300r/min.The weight of food-grade antioxidant peptide is 391g.
Result detects
In product, the content of protein, fat, carrotene measures as follows.
The detection of protein content is according to GB/T5009.5-2010 " mensuration of Protein in Food ", and nitrogen conversion factor calculates according to 6.24.
Determination of fat measures according to GB/T5009.6-2003 " mensuration fatty in food " soxhlet extraction.
The mensuration of carrotene measures according to the mensuration of GB8821-2011 " food additives bata-carotene " A4 bata-carotene.
The assay method of the holding time of carrotene is:
Kept in Dark Place by product in Fresco Bag, in 0,60,90,120 day, measure the content of carrotene wherein respectively, the assay method of content is as the assay method of GB8821-2011 " food additives bata-carotene " A4 bata-carotene.
The detection method of anti-oxidation peptide is carried out according to the appendix A of GB/T22492-2008 and the molecular vibrational temperature of the peptide of B and the content assaying method of peptide.
The constituent analysis of the food-grade antioxidant peptide of embodiment 1-3 is as follows:
The protein peptides of this explained hereafter high-selenium corn, does not have fat substantially, and protein content enriches, and has peptide matters high concentration, and grain is low, UA advantage in acid condition.Blood fat can be reduced, increases the excretion of cholesterol from enteron aisle.
Above-describedly be only preferred embodiment of the present invention, all do within the scope of the spirit and principles in the present invention any amendment, equivalently to replace and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. produce the method for food-grade antioxidant peptide with corn protein powder for one kind, it is characterized in that, described method is specially: be mixed with the corn protein powder solution that zein powder content is 5% ~ 8% after protein content being no less than the corn protein powder pulverizing of 50wt%, biology enzyme is adopted to carry out saccharification with the starch in amylorrhexis corn protein powder, after filtration after washing, collect filter residue and carry out saponification except degrease, again after filtration washing, collect filter residue, be hydrolyzed under compound protease existent condition, eventually pass centrifugal drying and obtain food-grade antioxidant peptide;
Wherein, the concrete grammar be hydrolyzed under compound protease existent condition is: containing filter residue 150 ~ 200g, pH6.0, temperature 40 ~ 50 DEG C, the consumption 1L/ ton filter residue of compound protease, 2 ~ 3 hours reaction time in often liter of hydrolyzate.
2. corn protein powder according to claim 1 produces the method for food-grade antioxidant peptide, it is characterized in that, described compound protease is the compound protease that Novozymes Company produces.
3. corn protein powder according to claim 1 produces the method for food-grade antioxidant peptide, and it is characterized in that, the concrete grammar of saponification is: the filter residue after filtration washing is diluted with water to 150 ~ 200g/L, then adds Na
2cO
3, be adjusted to pH8 ~ 10, under the condition of 45 ~ 55 DEG C, react a period of time.
4. corn protein powder according to claim 3 produces the method for food-grade antioxidant peptide, and it is characterized in that, the used time of saponification is 2 ~ 4h.
5. corn protein powder according to claim 1 produces the method for food-grade antioxidant peptide, it is characterized in that, the concrete grammar of the starch in amylorrhexis corn protein powder is: 105 DEG C ~ 110 DEG C, under the condition of pH5.6 ~ 6.0, with the starch in amylorrhexis corn protein powder, the chromogenic reaction depending on iodine liquid is colourless terminal of arriving.
6. corn protein powder according to claim 5 produces the method for food-grade antioxidant peptide, and it is characterized in that, the starch used time in amylorrhexis corn protein powder is 90 ~ 120 minutes.
7. corn protein powder according to claim 1 produces the method for food-grade antioxidant peptide, it is characterized in that, described amylase is the High-temperature Liquefaction amylase that Jie Nengke bioengineering Co., Ltd produces, and the diastatic consumption of described High-temperature Liquefaction is 1L/ ton corn protein powder.
8. corn protein powder according to claim 1 produces the method for food-grade antioxidant peptide, it is characterized in that, described saccharification biology enzyme used is carbohydrase, the concrete grammar of described saccharification is: regulate pH to be 4.5 ~ 5.5 the solution through amylorrhexis, temperature 60 C, saccharificatinn period is 18 ~ 24h.
9. corn protein powder according to claim 1 produces the method for food-grade antioxidant peptide, and it is characterized in that, after saponification, the temperature of filtration washing is 75 DEG C.
10. corn protein powder according to claim 1 produces the method for food-grade antioxidant peptide, and it is characterized in that, described corn protein powder comprises following component: protein >=50wt%; Starch≤18wt%; Fat≤8wt%; Fiber≤3wt%; Ash content≤4wt%; Water≤5wt%.
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| CN113943768A (en) * | 2021-09-27 | 2022-01-18 | 齐齐哈尔易翔食品有限公司 | Separation and purification method of corn antioxidant peptide |
| CN114150033A (en) * | 2021-12-06 | 2022-03-08 | 哈尔滨商业大学 | Preparation method of rice bran antioxidant peptide compound treated by peak alpha amylase |
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| CN114150033A (en) * | 2021-12-06 | 2022-03-08 | 哈尔滨商业大学 | Preparation method of rice bran antioxidant peptide compound treated by peak alpha amylase |
| CN114150033B (en) * | 2021-12-06 | 2023-05-19 | 哈尔滨商业大学 | Preparation method of rice bran antioxidant peptide compound treated by peak alpha amylase |
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