CN104944594A - Preparation method of microbial flocculant and application in treatment of potato starch wastewater - Google Patents
Preparation method of microbial flocculant and application in treatment of potato starch wastewater Download PDFInfo
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- CN104944594A CN104944594A CN201510269635.6A CN201510269635A CN104944594A CN 104944594 A CN104944594 A CN 104944594A CN 201510269635 A CN201510269635 A CN 201510269635A CN 104944594 A CN104944594 A CN 104944594A
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Abstract
The invention discloses a preparation method of a microbial flocculant. Rhodococcus erythropolis is adopted as a strain and the sterilized potato starch wastewater is used as a culture medium; nutrient substances are added for fermentation; and the supernate obtained after centrifuging the fermented liquid is the microbial flocculant. A large number of experiments prove that the removal rate of COD in the potato starch wastewater by the microbial flocculant prepared by the method disclosed by the invention is over 99% and completely reaches the national discharge standard.
Description
Technical field
The present invention relates to a kind of preparation of microbial flocculant, particularly relate to a kind of potato starch wastewater that utilizes as the method for medium preparing microbial flocculant, be mainly used in the process of potato starch wastewater.
Background technology
Microbial flocculant is the macromolecule organic with flocculating function that a class is produced by microorganism.Mainly contain glycoprotein, mucopolysaccharide, Mierocrystalline cellulose and nucleic acid etc.From its source, also belong to natural organic high-molecular flocculant, therefore it has all advantages of natural organic high-molecular flocculant.Microbial flocculant has very strong flocculation activity, and can biological degradation, non-secondary pollution, the advantage such as easy to use, is widely used in the process of livestock products waste water, bulking mud, colorful wastewater, municipal effluent, building materials waste water, food wastewater, hospital wastewater, petrochemical wastewater, paper waste and starch wastewater.
The current research work for microbial flocculant has been entered into by purification, modification and has utilized biotechnology to cultivate, screen excellent bacterial classification, obtains the research of efficient flocculation agent, and achieve certain effect with lower cost.But its production cost is too high, less in actual applications.Domestic and international investigator, in order to reduce the preparation cost of microbial flocculant, has selected gourmet powder waste water, fish meal wastewater, sauce waste water, glutinous rice waste water etc. as substratum, although achieve certain achievement, effect is not too desirable.
Yam starch in process of production, is on average often produced the potato that 1 ton of starch needs consumption about 6.5 tons, is discharged the waste water of about 20 tons, the potato slag of about 5 tons.Produce a large amount of technique organic waste water in the process, mainly solvability starch and protein, COD is usually at 8000 ~ 30000mg/g, protein content is at 2000 ~ 8000mg/g, high-concentration waste water like this directly enters in the Nature, not only serious pollution is caused to environment, and be the waste to water resources.If the zero release of potato starch wastewater can be realized, can not only be Reducing Cost in Enterprises, the more important thing is and can save valuable water resources.
The treatment process of current potato waste water is more, wherein flocculence is also one of conventional method, uses maximum flocculation agents to be aluminum chloride hydrocarbon complex at present, bodied ferric sulfate etc., although with low cost, handled waste water appoint so do not reach national regulation discharge or Enterprises service requirements.Microbial flocculant process potato waste water is utilized also to have scientific research personnel to be studied report: to utilize the active sludge of secondary sedimentation tank of sewage treatment work isolated Candida anglica fungi, with the potato starch wastewater of sterilized for substratum, the COD clearance of microbial flocculant to potato starch wastewater of preparation is 93.7%, (be reduced to 562mg/L from the 8862mg/L of raw wastewater, but still do not have a certain distance higher than 150mg/L with the COD value that specifies in discharging standards GB 25461-2010).China is yam starch big producing country, remains very urgent task to the process of potato starch wastewater, and therefore, the microbial flocculant finding high efficiency, low cost needs to continue research and probe for the treatment of starch wastewater.
Summary of the invention
The object of the invention is for problems of the prior art, a kind of preparation method of microbial flocculant is provided;
Another object of the present invention provides the application in this microbial flocculant process potato starch wastewater a kind of;
One, the preparation of microbial flocculant
The preparation method of microbial flocculant of the present invention take rhodococcus erythropolis as bacterial strain, and with the potato starch wastewater of sterilizing for substratum, add nutritive substance and ferment, the supernatant liquor after fermentation liquor is centrifugal is microbial flocculant.
The COD value of potato starch wastewater substratum is at 5000 ~ 5500mg/L; The access amount of rhodococcus erythropolis is: 50 ~ 250mL/L substratum;
Nutritive substance be sheep urine/horse urine with the mixture of ammonium hydrogen phosphate (sheep urinate/horse urinates and the mass ratio of ammonium hydrogen phosphate is 1:1 ~ 1:10; The addition of nutritive substance is 22 ~ 77g/L substratum;
Fermentation condition is: constant temperature oscillation fermentation 50 ~ 75h at 15 ~ 40 DEG C;
Described centrifugal speed is 5000 ~ 8000 r/min.
Two, the application of microbial flocculant
Using method: add 5mL microbial flocculant in 1L potato starch wastewater (COD value is 8976mg/L, without sterilizing), stirs and leaves standstill 0.2h, measures the COD value of potato starch wastewater.
Result of use: be 132mg/L by the COD value of potato starch wastewater after this microbial flocculant process, reaches 99.98% to the clearance of COD.
Experimental results demonstrate, microbial flocculant prepared by the present invention all more than 99%, reaches discharging standards to the clearance of potato starch wastewater COD completely.
Embodiment
Be further described below by the Synthesis and applications effect of specific embodiment to microbial flocculant of the present invention.
Embodiment 1
(1) preparation of microbial flocculant: the transfering loop picking flocculation agent of the bacterial classification in seed culture medium is produced bacterium rhodococcus erythropolis 250mL, accesses in 1 L potato starch wastewater (COD is 5000mg/L, sterilizing) substratum; Add the mixture (70g ammonium hydrogen phosphate/7g sheep urinates) of 77g sheep urine and ammonium hydrogen phosphate again, constant temperature oscillation fermentation 75h at 40 DEG C; Fermentation liquor centrifugal (8000 r/min), supernatant liquor is microbial flocculant;
(2) application of microbial flocculant: (COD value is 8976mg/L at 1L potato starch wastewater, without sterilizing) the middle microbial flocculant adding the above-mentioned preparation of 5mL, stir and leave standstill 0.2h, the COD value measuring potato starch wastewater is 132mg/L, reaches 99.98% to the clearance of COD.
Embodiment 2
(1) preparation of microbial flocculant: the transfering loop picking flocculation agent of the bacterial classification in seed culture medium is produced bacterium rhodococcus erythropolis 50mL, in access 1L potato starch wastewater (COD is 5500mg/L, sterilizing) substratum; Add the mixture (20g ammonium hydrogen phosphate/2g sheep urinates) of 22 sheep urine and ammonium hydrogen phosphate again, constant temperature oscillation fermentation 50h at 15 DEG C; Fermentation liquor centrifugal (5000 r/min), supernatant liquor is microbial flocculant;
(2) application of microbial flocculant: (COD value is 8976mg/L at 1L potato starch wastewater, without sterilizing) the middle microbial flocculant adding the above-mentioned preparation of 5mL, stir and leave standstill 0.2h, the COD value measuring potato starch wastewater is 148mg/L, reaches 99.98% to the clearance of COD.
Embodiment 3
(1) preparation of microbial flocculant: the transfering loop picking flocculation agent of the bacterial classification in seed culture medium is produced bacterium rhodococcus erythropolis 100mL, in access 1L potato starch wastewater (COD is 5300mg/L, sterilizing) substratum; Add the mixture (33g ammonium hydrogen phosphate/33g horse urinates) of 66 horse urine and ammonium hydrogen phosphate again, constant temperature oscillation fermentation 60h at 35 DEG C; Fermentation liquor centrifugal (7000 r/min), supernatant liquor is microbial flocculant;
(2) application of microbial flocculant: (COD value is 8976mg/L at 1L potato starch wastewater, without sterilizing) the middle microbial flocculant adding the above-mentioned preparation of 5mL, stir and leave standstill 0.2h, the COD value measuring potato starch wastewater is 127mg/L, reaches 99.98% to the clearance of COD.
Embodiment 4
(1) preparation of microbial flocculant: the transfering loop picking flocculation agent of the bacterial classification in seed culture medium is produced bacterium rhodococcus erythropolis 200mL, in access 1L potato starch wastewater (COD is 5400mg/L, sterilizing) substratum; Add the mixture (50g ammonium hydrogen phosphate/5g sheep urinates) of 55 sheep urine and ammonium hydrogen phosphate again, constant temperature oscillation fermentation 65h at 25 DEG C; Fermentation liquor centrifugal (7500 r/min), supernatant liquor is microbial flocculant;
(2) application of microbial flocculant: (COD value is 8976mg/L at 1L potato starch wastewater, without sterilizing) the middle microbial flocculant adding the above-mentioned preparation of 5mL, stir and leave standstill 0.2h, the COD value measuring potato starch wastewater is 119mg/L, reaches 99.99% to the clearance of COD.
Embodiment 5
(1) preparation of microbial flocculant: the transfering loop picking flocculation agent of the bacterial classification in seed culture medium is produced bacterium rhodococcus erythropolis 120mL, in access 1L potato starch wastewater (COD is 5400mg/L, sterilizing) substratum; Add the mixture (35g ammonium hydrogen phosphate/5g horse urinates) of 40 horse urine and ammonium hydrogen phosphate again, constant temperature oscillation fermentation 65h at 25 DEG C; Fermentation liquor centrifugal (7500 r/min), supernatant liquor is microbial flocculant;
(2) application of microbial flocculant: (COD value is 8976mg/L at 1L potato starch wastewater, without sterilizing) the middle microbial flocculant adding the above-mentioned preparation of 5mL, stir and leave standstill 0.2h, the COD value measuring potato starch wastewater is 139mg/L, reaches 99.98% to the clearance of COD.
Claims (8)
1. a preparation method for microbial flocculant take rhodococcus erythropolis as bacterial strain, and the potato starch wastewater of sterilizing is substratum, and ferment under interpolation nutritive substance, the supernatant liquor after fermentation liquor is centrifugal is microbial flocculant.
2. the preparation method of microbial flocculant as claimed in claim 1, is characterized in that: the COD value of described potato starch wastewater substratum is at 5000 ~ 5500mg/L.
3. the preparation method of microbial flocculant as claimed in claim 1 or 2, is characterized in that: the access amount of described bacterial strain rhodococcus erythropolis is: 50 ~ 250mL/L substratum.
4. the preparation method of microbial flocculant as claimed in claim 1 or 2, is characterized in that: described nutritive substance is sheep urine/horse urine and the mixture of ammonium hydrogen phosphate, and the addition of nutritive substance is 22 ~ 77g/L substratum.
5. the preparation method of microbial flocculant as claimed in claim 4, is characterized in that: sheep urine/horse urine with the mass ratio of ammonium hydrogen phosphate is: 1:1 ~ 1:10.
6. the preparation method of microbial flocculant as claimed in claim 1 or 2, is characterized in that: described fermentation condition is: constant temperature oscillation fermentation 50 ~ 75h at 15 ~ 40 DEG C.
7. the preparation method of microbial flocculant as claimed in claim 1 or 2, is characterized in that: described centrifugal speed is 5000 ~ 8000 r/min.
8. the application as claimed in claim 1 in the microbial flocculant process potato starch wastewater prepared of method.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105238843A (en) * | 2015-11-09 | 2016-01-13 | 成都信息工程大学 | Microbial flocculant, preparation method and application thereof |
| CN105776574A (en) * | 2016-05-20 | 2016-07-20 | 佛山市聚成生化技术研发有限公司 | Microbial flocculant and application thereof in slaughter wastewater treatment |
| CN106587538A (en) * | 2017-01-10 | 2017-04-26 | 上海应用技术大学 | Method for treating excrement and urine wastes |
Citations (3)
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| JPH01211492A (en) * | 1988-02-18 | 1989-08-24 | Agency Of Ind Science & Technol | Enhanced production of microorganism flocculant noc-1 |
| CN101805707A (en) * | 2009-03-23 | 2010-08-18 | 中山大学 | Producing strain for producing microbial flocculating agent by using starch wastewater and production process thereof |
| CN102559768A (en) * | 2012-02-09 | 2012-07-11 | 湖南大学 | Two-step fermentation production method of microbial flocculant |
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2015
- 2015-05-25 CN CN201510269635.6A patent/CN104944594A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01211492A (en) * | 1988-02-18 | 1989-08-24 | Agency Of Ind Science & Technol | Enhanced production of microorganism flocculant noc-1 |
| CN101805707A (en) * | 2009-03-23 | 2010-08-18 | 中山大学 | Producing strain for producing microbial flocculating agent by using starch wastewater and production process thereof |
| CN102559768A (en) * | 2012-02-09 | 2012-07-11 | 湖南大学 | Two-step fermentation production method of microbial flocculant |
Non-Patent Citations (3)
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| 王有乐等: "淀粉废水培养复合型微生物絮凝剂产生菌研究", 《工业水处理》 * |
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| 颜东方等: "马铃薯淀粉废水生产微生物絮凝剂菌株筛选及其营养条件优化", 《农业工程学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105238843A (en) * | 2015-11-09 | 2016-01-13 | 成都信息工程大学 | Microbial flocculant, preparation method and application thereof |
| CN105238843B (en) * | 2015-11-09 | 2018-08-21 | 成都信息工程大学 | A kind of microbial flocculant, preparation method and applications |
| CN105776574A (en) * | 2016-05-20 | 2016-07-20 | 佛山市聚成生化技术研发有限公司 | Microbial flocculant and application thereof in slaughter wastewater treatment |
| CN106587538A (en) * | 2017-01-10 | 2017-04-26 | 上海应用技术大学 | Method for treating excrement and urine wastes |
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Application publication date: 20150930 |