CN104938820A - Preparation method for semi-solid ctenopharyngoden idella feed with high palatability - Google Patents
Preparation method for semi-solid ctenopharyngoden idella feed with high palatability Download PDFInfo
- Publication number
- CN104938820A CN104938820A CN201510309769.6A CN201510309769A CN104938820A CN 104938820 A CN104938820 A CN 104938820A CN 201510309769 A CN201510309769 A CN 201510309769A CN 104938820 A CN104938820 A CN 104938820A
- Authority
- CN
- China
- Prior art keywords
- compound
- feed
- powder
- ctenopharyngoden
- idella
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention discloses a preparation method for a semi-solid ctenopharyngoden idella feed with high palatability. The preparation method is simple and convenient to operate, easy to popularize, low in manpower and material resource consumption, low in energy consumption, wide in raw material source, low in production investment, low in cost and wide in application range; the prepared feed can promote the growth of ctenopharyngoden idella, increase the survival rate of ctenopharyngoden idella, reduce the death and elimination rate, improve the feed efficiency, reduce the input cost, and realize the high-yield and efficient culture of ctenopharyngoden idella; according to the preparation method, reasonable use of fish products and fish product leftovers can be realized, environmental pollution is reduced, the use of antibiotics in the feed is reduced, and the environmental protection property and safety of fish products can be ensured.
Description
Technical field
The present invention relates to aquatic feeds preparation field, be specifically related to the preparation method of the strong semi-solid feed of grass carp of a kind of palatability.
Background technology
Containing rich in protein in fishery-ies product and leftover bits and pieces thereof, be aquatic feeds by its Development and Production for alleviating environmental pollution, to realize the comprehensive utilization of discarded object significant.Raw material fish is through being hydrolyzed the liquid protein obtained, its nutritional labeling is close with high-quality fish meal nutritional labeling, and the content of its lysine contained, methionine is all higher than fish meal, contained essential amino acid A wide selection of colours and designs, ratio are suitable, and the content of vitamin and trace element is also very abundant.For fish meal, fish silage not only wide in variety, function is many, have no side effect, aromatic flavour, and its processing and fabricating is simple, and production cost is low, and required human and material resources are few, and raw material is extensive, applied widely.
The biologically active peptide that glutathione (glutathione, GSH) is made up of gamma-glutamic acid, cysteine, glycine, is divided into reduced glutathione and oxidized form of glutathione.Usually alleged glutathione refers to reduced glutathione, be the important active substances in body, there is scavenging free radicals, removing toxic substances, promotion irony absorb and maintain the integrality of erythrocyte membrane, maintain the different physiological roles such as the biosynthesis of DNA, the normal growth of cell and cellular immunity.At present, glutathione is studied more in Production of Livestock and Poultry, has confirmed that glutathione can improve the cleavage rates of porcine oocytes maturing rate and Microfertilization embryo, improves the milk performance of lactating mammal, remove aflatoxin to the toxicity of chick, remove the nosotoxicosis etc. that fish body Microcystin causes.Up to now, relevant glutathione is applied seldom in aquatic animal feed, then more rare about the application of glutathione in feed of grass carp.
Grass carp (Ctenophary ngodon idella) is Chinese important freshwater aquiculture economic fish, and nutritious, economic worth is high.Annual production accounts for 30% of CHINESE FRESHWATER fish total output, annual value of production more than 50,000,000,000 yuan.Grass carp also as export varieties, more than 10 countries and regions such as Romania, Hong Kong, Macao of being in great demand.But grass carp is low vertebrate such as grade, and its function of immune system is low, and disease very easily outbreak of epidemic, supports adult fish from fry, and survival rate is less than 30%.Due to glutathione, there is antioxidation and integrate the effects such as removing toxic substances, can also help to keep normal immune function, therefore, appropriate glutathione can be added to strengthen its premunition in feed of grass carp, improve its survival rate, realize the high yield of grass carp, fecund.
Summary of the invention
In order to the highly efficient and productive cultivation of the Appropriate application and grass carp that realize fishery-ies product and leftover bits and pieces thereof, the invention provides a kind of new feed manufacturing preparation method, the inventive method is, by lactobacillus-fermented, fish discarded object is converted into liquid protein, finally be prepared into through series of steps and can improve immunity function and be convenient to the semi-solid feed of grass carp of ingesting, technical scheme of the present invention is as follows:
A preparation method for the semi-solid feed of grass carp that palatability is strong, method is as follows:
(1) ratio of 1: 1 takes food grade glucose and each portion of food grade sucrose in mass ratio, add water and be made into the sugar juice that mass concentration is 2%, again by 1% inoculum concentration by lactobacillus inoculum in sugar juice, cultivate activation under being placed in the condition of 30 DEG C 24 hours, obtain lactobacillus suspension;
(2) fish discarded object is rubbed, add the leftover bits and pieces of molasses or cane sugar manufacture in the ratio of its weight 15% and stir, obtaining compound;
(3) being spilled into by the lactobacillus suspension of step (1) in the compound of step (2) and stirring, the consumption of lactobacillus suspension is 2% of compound gross weight;
(4) anaerobic fermentation is carried out, fermentation time about 30 days by the compound of step (3) loading closed container;
(5) add in the product obtained at anaerobic fermentation the wheat flour of its weight 20-40%, swelling soya dreg powder, spirulina powder, straw bran powder compound and stir, wherein wheat flour, swelling soya dreg powder, spirulina powder, straw bran powder are the ultra-micro powder of more than 100 orders;
(6) compound of step (5) is heated to 50-60 DEG C while stirring, insulation continues to stir 20-30 minute at this temperature;
(7) reduced glutathione to be joined in the compound of step (6) and to make its final concentration be 200mg/kg;
(8) agar powder getting the compound gross weight 2.5% of step (7) is heated to 95 DEG C of fusings after adding the dilution of a small amount of water, agar after fusing is joined in compound and stirs, then pour into while hot in mould, treat that it cools, semi-solid feeds.
Beneficial effect of the present invention:
1, preparation method of the present invention is easy and simple to handle, be easy to popularize, required human and material resources are few, energy resource consumption is low, raw material are extensive, and investment of production is few, cost is low, applied widely, not only can promote that Growth of Grass Carps Ctenopharyngodon Idellus is grown, improve the survival rate of grass carp, reduce death rate, improve feed efficiency, save input cost, realize the highly efficient and productive cultivation of grass carp, and the Appropriate application of fishery-ies product and leftover bits and pieces thereof can be realized, alleviate environmental pollution, reduce antibiotic use in feed, ensure the green safety of processed fish meat products.
2, the semi-solid feeds obtained by the present invention is better than traditional pellet palatability, and digestibility is higher, and attractant is better, environmental protection more more economical than pellet.
3, the principle of lactobacillus-fermented is lactic acid bacteria amount reproduction produce a large amount of lactic acid under the environment of airtight anoxia, reduce the pH value of fish discarded object, inhibit the growth of spoilage organisms, simultaneously because the effect of lactic acid makes fish discarded object liquefaction self-dissolving, breaks down proteins, amino acid, vitamin and micro-stripping, add the nutrient content of feed, improve digestibility.
4, reduced glutathione (GSH) can the poisonous substance such as scavenging free radicals, peroxide, heavy metal, Microcystin and aflatoxin; promote the absorption of Fe, inorganic Se and Ca; protection gastrointestinal tract mucosa epithelium; prevent the damage because inflammation, ischaemic, noxious material, oxidation material etc. cause intestinal mucosa; it plays an important role in the anti-infective and inflammatory reaction of immune system; and participate in the adjustment of the cell factor such as leukotrienes, MIF, IL-2; promote lymphocyte and mononuclear cell proliferation, thus affect the physiological function of body.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the present invention, every simple distortion that other are made when not departing from core of the present invention or amendment all fall into protection scope of the present invention.
A preparation method for the semi-solid feed of grass carp that palatability is strong, method is as follows:
(1) ratio of 1: 1 takes food grade glucose and each portion of food grade sucrose in mass ratio, add water and be made into the sugar juice that mass concentration is 2%, again by 1% inoculum concentration by lactobacillus inoculum in sugar juice, cultivate activation under being placed in the condition of 30 DEG C 24 hours, obtain lactobacillus suspension;
(2) fish discarded object is rubbed, add molasses in the ratio of its weight 15% and stir, obtaining compound;
(3) being spilled into by the lactobacillus suspension of step (1) in the compound of step (2) and stirring, the consumption of lactobacillus suspension is 2% of compound gross weight;
(4) anaerobic fermentation is carried out, fermentation time about 30 days by the compound of step (3) loading closed container;
(5) add in the product obtained at anaerobic fermentation the wheat flour of its weight 30%, swelling soya dreg powder, spirulina powder, straw bran powder compound and stir, wherein wheat flour, swelling soya dreg powder, spirulina powder, straw bran powder are the ultra-micro powder of more than 100 orders;
(6) compound of step (5) is heated to 50-60 DEG C while stirring, insulation continues stirring 30 minutes at this temperature;
(7) reduced glutathione to be joined in the compound of step (6) and to make its final concentration be 200mg/kg;
(8) agar powder getting the compound gross weight 2.5% of step (7) is heated to 95 DEG C of fusings after adding the dilution of a small amount of water, agar after fusing is joined in compound and stirs, then pour into while hot in mould, treat that it cools, semi-solid feeds.
Positive role of the present invention is further illustrated below in conjunction with test examples:
(1) fish is tested: average weight is about the young grass carps of 8.5g.
(2) test method: 300 tail young grass carps are divided at random control group and test group two groups, control group and test group respectively establish 3 to repeat, each repetition 50 tail, raises in the circular water flow filter aquarium of 300L.Control group feed normal diet, test group feed the made feed of the present invention, duration of test is is quantitatively thrown something and fed, and every day throws something and feeds for 3 times respectively at 8: 30,11: 30 and 16: 30 points, day feeding volume be 4% of weight.Observe a fish health status every day, record death condition, within every 2 weeks, weigh 1 time, experimental period is 8 weeks.Water temperature 18-30 DEG C, pH 7.5, dissolved oxygen is higher than 5mg/L, and ammonia nitrogen is lower than 0.02mg/L.
(3) Test Data Collecting: during off-test, adds up the amount of survival of every case grass carp and weighs the oeverall quality of every case grass carp, calculating specific growth rate, survival rate, the feed efficiency of grass carp.10 tail grass carps are got in each repetition at random, get blood, be divided into 2 parts with aseptic 10mL syringe and No. 5 syringe needles from arteria caudalis, with anticoagulant heparin, prepare anticoagulation for measuring number of white blood cells for 1 part; 1 part of blood is placed in centrifuge tube, through the centrifugal 5min of 4000r/min, gets supernatant determination of plasma insulin-like growth factor-i (IGF-1).Get the 5 tail grass carps being pumped through blood, dissect, get liver pancreas and weigh, measure liver pancreas index.
(4) test result analysis:
Table 1: feed normal diet and the made feed of the present invention are on the impact of grass carp specific growth rate, survival rate, feed efficiency
Note: in table in same hurdle, has significant difference (P<0.05) between the data that the upper right corner indicates different English alphabet.
Specific growth rate (%)=(ln opisthosoma body weight-ln is body body weight just)/raise number of days × 100;
Survival rate (%)=(end number-initial mantissa)/initial mantissa × 100;
Gross mass × 100 are raised in feed efficiency (%)=(opisthosoma body weight-first body body weight)/throwing.
From table 1, the grass carp of the made feed ratio normal diet of the present invention that feeds all is significantly increased in specific growth rate, survival rate, feed efficiency, illustrates that the made feed of the present invention can promote growing of grass carp, has apparent positive role.
Table 2: the liver pancreas index of the grass carp of feed normal diet and the made feed of the present invention, insulin-like growth factor-i content and white blood cell count(WBC)
Note: in table in same hurdle, has significant difference (P<0.05) between the data that the upper right corner indicates different English alphabet.
From table 2, the made feed of the present invention is owing to the addition of 200mgkg
-1reduced glutathione (GSH), the grass carp thus fed out its liver pancreas index, insulin-like growth factor-i and white blood cell count(WBC) all significantly improve than control group.
The liver pancreas index of fish is that the g and D of liver is often subject to the impact of feed ingredient to long-term and that short-term nutritional mode is all very sensitive index.Compared with control group, test group Grass Carp Liver pancreas index has and raises in various degree, and this may be the synthesis that GSH facilitates liver cell DNA, and then shows that Grass Carp Liver pancreas index increases to some extent.Large quantity research confirms, insulin-like growth factor-i (IGF-1) major part in blood circulation is by liver synthesis secretion.And the test group grass carp IGF-1 activity of the made feed of the present invention of ingesting in this test significantly improves, show that GSH facilitates the ability of liver synthesis and secretion IGF-1, further enhancing liver cell DNA synthesis capability, finally improve liver pancreas index.
IGF-1 biological agent in blood circulation is only by the adjustment of growth hormone, and the growth promoting function of growth hormone is mediated by IGF-1, in this test, the raising of control group grass carp IGF-1 level illustrates that in feed of grass carp, add GSH can regulate grass carp growth hormone level, and finally reaches the effect promoting Growth of Grass Carps Ctenopharyngodon Idellus.This also just significantly improves corresponding with grass carp specific growth rate, feed efficiency in test.
The neutrophil of fish and monocyte have been proved has very strong phagocytic activity, and for specific immunity function, this ability is even more important for the aquatic animal of lower grade.Leukocytic phagocytic function is the important component part of animal nospecific immunity.Containing lysozyme, proteolytic enzyme, MPer etc. in leukocytic lysosome, these enzymes are lethal to most of microbe.After leucocyte is by suitable stimulation, prostaglandin, thromboxane and trienic acid can be produced.Therefore, the number of white blood cells measuring animal can reflect animal non-specific immunity to a certain extent.In Fish Blood, number of white blood cells may contact antigenicity substance because of body or be subject to the stimulation of envirment factor and rise.This result of the test shows, in feed of grass carp, add GSH can increase number of white blood cells in Blood of Ctenopharyngodon, this just improves corresponding with grass carp survival rate in test, illustrate that GSH passes through to stimulate the leukocytic generation of Blood of Ctenopharyngodon thus the nonspecific defense function that further enhancing grass carp, and then reach the effect strengthening premunition.
Claims (1)
1. a preparation method for the semi-solid feed of grass carp that palatability is strong, it is characterized in that, method is as follows:
(1) ratio of 1: 1 takes food grade glucose and each portion of food grade sucrose in mass ratio, add water and be made into the sugar juice that mass concentration is 2%, again by 1% inoculum concentration by lactobacillus inoculum in sugar juice, cultivate activation under being placed in the condition of 30 DEG C 24 hours, obtain lactobacillus suspension;
(2) fish discarded object is rubbed, add the leftover bits and pieces of molasses or cane sugar manufacture in the ratio of its weight 15% and stir, obtaining compound;
(3) being spilled into by the lactobacillus suspension of step (1) in the compound of step (2) and stirring, the consumption of lactobacillus suspension is 2% of compound gross weight;
(4) anaerobic fermentation is carried out, fermentation time about 30 days by the compound of step (3) loading closed container;
(5) add in the product obtained at anaerobic fermentation the wheat flour of its weight 20-40%, swelling soya dreg powder, spirulina powder, straw bran powder compound and stir, wherein wheat flour, swelling soya dreg powder, spirulina powder, straw bran powder are the ultra-micro powder of more than 100 orders;
(6) compound of step (5) is heated to 50-60 DEG C while stirring, insulation continues to stir 20-30 minute at this temperature;
(7) reduced glutathione to be joined in the compound of step (6) and to make its final concentration be 200 mg/ kg;
(8) agar powder getting the compound gross weight 2.5% of step (7) is heated to 95 DEG C of fusings after adding the dilution of a small amount of water, is joined in compound by the agar after melting and stirs, then pour into while hot in mould, treat that it cools, semi-solid feeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510309769.6A CN104938820A (en) | 2015-06-06 | 2015-06-06 | Preparation method for semi-solid ctenopharyngoden idella feed with high palatability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510309769.6A CN104938820A (en) | 2015-06-06 | 2015-06-06 | Preparation method for semi-solid ctenopharyngoden idella feed with high palatability |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104938820A true CN104938820A (en) | 2015-09-30 |
Family
ID=54154346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510309769.6A Pending CN104938820A (en) | 2015-06-06 | 2015-06-06 | Preparation method for semi-solid ctenopharyngoden idella feed with high palatability |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104938820A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105380005A (en) * | 2015-11-26 | 2016-03-09 | 华南农业大学 | Moringa oleifera fish feed and preparation method of moringa oleifera fish feed as well as culturing method of fish |
| CN105876163A (en) * | 2016-06-28 | 2016-08-24 | 张家银 | Loach coated feed capable of realizing soybean meal fermentation modification by sophora japonica polysaccharides and improving delicious degree of fish and preparation method for loach coated feed |
| CN105981995A (en) * | 2016-01-07 | 2016-10-05 | 凤阳县兴科农业生态发展有限公司 | Preparation method lactic acid bacteria fermented wet-soft feed for grass carp |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734501A (en) * | 2013-12-09 | 2014-04-23 | 苏州市相城区新时代特种水产养殖场 | Grass carp feed, and preparation method thereof |
| CN104222677A (en) * | 2014-09-30 | 2014-12-24 | 浙江海洋学院 | Special feed for juvenile fish of plagiognathops microlepis and preparation method thereof |
-
2015
- 2015-06-06 CN CN201510309769.6A patent/CN104938820A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734501A (en) * | 2013-12-09 | 2014-04-23 | 苏州市相城区新时代特种水产养殖场 | Grass carp feed, and preparation method thereof |
| CN104222677A (en) * | 2014-09-30 | 2014-12-24 | 浙江海洋学院 | Special feed for juvenile fish of plagiognathops microlepis and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 周辛平等主编: "《猪、鸡、鱼养殖》", 30 April 2012, 甘肃文化出版社 * |
| 赵红霞等: "饲料中添加谷胱甘肽对草鱼生长、生理指标和抗病力的影响", 《中国水产科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105380005A (en) * | 2015-11-26 | 2016-03-09 | 华南农业大学 | Moringa oleifera fish feed and preparation method of moringa oleifera fish feed as well as culturing method of fish |
| CN105981995A (en) * | 2016-01-07 | 2016-10-05 | 凤阳县兴科农业生态发展有限公司 | Preparation method lactic acid bacteria fermented wet-soft feed for grass carp |
| CN105876163A (en) * | 2016-06-28 | 2016-08-24 | 张家银 | Loach coated feed capable of realizing soybean meal fermentation modification by sophora japonica polysaccharides and improving delicious degree of fish and preparation method for loach coated feed |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103141699B (en) | Novel feed for cattle and sheep and preparation method of novel feed | |
| CN109247447B (en) | Yeast hydrolysate for replacing plasma protein powder to produce milk replacer and preparation method thereof | |
| CN101897317B (en) | Culture method of medicated worm grass pigs | |
| CN102669483A (en) | Mixed plant protein fish feed as well as preparation method and application thereof | |
| CN105166456A (en) | Micropterus salmonides compound feed for substituting iced and fresh fish baits | |
| CN106071420A (en) | The preparation method of a kind of trace element chelated peptide and Litopenaeus vannamei breeding feed | |
| CN106173270A (en) | A kind of compound micro-ecological preparation and application thereof | |
| CN104938820A (en) | Preparation method for semi-solid ctenopharyngoden idella feed with high palatability | |
| CN104938776A (en) | Preparation method for wet and soft feed capable of improving survival rate of ctenopharyngoden idella | |
| CN104920851A (en) | Preparation method for granular fish feed capable of enhancing resistance to disease | |
| CN101961330A (en) | Anti-stress agent for aquatic livestock | |
| CN107996888A (en) | A kind of Procambius clarkii is into new disease-resistant additive of shrimp and preparation method thereof | |
| CN108157591A (en) | A kind of preparation method for promoting standing grain flower carp meat nonreactive feed | |
| CN107712273A (en) | Improve feed of bream anti-stress ability and preparation method thereof | |
| CN108244400A (en) | A kind of compound feed for snakeheads and preparation method thereof | |
| CN104938821A (en) | Preparation method for fish feed liquid capable of replacing antibiotics | |
| CN103283939A (en) | Solid fermentation preparation method for yeast protein feed | |
| KR100557467B1 (en) | Process for feeding fish and livestock a liquid extract of mushroom mycelial culture and product thereof | |
| CN105941836A (en) | Preparation method of lactobacillus-fermented liquid fish feed | |
| CN105475697A (en) | Preparation method of wet and soft feed for grass carps | |
| CN102172264B (en) | Albumen powder for replacing plasma power in pig starter feed and method for preparing same | |
| CN104206649A (en) | Green biological aquatic feed and production method thereof | |
| CN103960462A (en) | Method for preparing polypeptide feed through wine-making yeast | |
| CN106720929A (en) | For the preparation method of bacterium peptide rice feed of the fermenting and producing rich in taurine | |
| CN106376772A (en) | Prawn feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150930 |
|
| RJ01 | Rejection of invention patent application after publication |