CN104938333A - Simple aseptic plant culture box and use thereof - Google Patents
Simple aseptic plant culture box and use thereof Download PDFInfo
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- CN104938333A CN104938333A CN201510303082.1A CN201510303082A CN104938333A CN 104938333 A CN104938333 A CN 104938333A CN 201510303082 A CN201510303082 A CN 201510303082A CN 104938333 A CN104938333 A CN 104938333A
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Abstract
The invention provides a simple aseptic plant culture box. The simple aseptic plant culture box comprises a box, a fluorescent lamp, a UV lamp, four air filters, a sucking pump, a culture base, a culture pipe, a high efficiency air filter, two syringes, a water inlet pipe and a water outlet pipe. The culture pipe and the culture base are arranged in the box. The fluorescent lamp and the UV lamp are installed on the top of the box. One side of the box is orderly provided with the air filters and the high efficiency air filter and the other side is provided with the air filters and the sucking pump. One end of the water inlet pipe is located above the culture pipe and the other end of the water inlet pipe is connected to the air filters and the syringes. The bottom of the culture pipe is connected to the water outlet pipe and the other end of the water outlet pipe is connected to the air filters and the syringes. The simple aseptic plant culture box has a reasonable design, can be prepared simply, has a low cost, is convenient for operation, can be used for soil culture and water culture, effectively prevents microbe-caused change of plant growth and culture medium components, has good disinfection effects and provides an aseptic environment for plant growth for a long time.
Description
Technical field
The invention belongs to and asepticly plant training field, particularly relate to and a kind ofly simple and easyly asepticly plant training case and the application in aseptic culture thereof.
Background technology
Microorganism participates in oxidation, nitrated, ammonification, fixed nitrogen, sulfidation, promote the decomposition of organic substance and the conversion of nutrient in natural soils, and it all extensively distributes in soil, water body and air.Soil has possessed the conditions such as the nutrition required for various growth of microorganism growth, moisture, air, acid-base value, osmotic pressure and temperature, and be the good environment of microorganism life, therefore maximum in Soil Microorganism quantity, type is maximum.Microorganism in fresh water comes from soil, air, sewage or animals and plants corpse etc. more, and the microorganism especially in soil, is often entered in rivers, lake by rain drop erosion with soil.Containing the microorganism of some from soil, biology and water body etc. in air, it is brought by air-flow in the mode such as dust, particulate.Therefore, the distribution of microorganism is very extensive, ubiquitous.
But specific production and scientific research then need the environment of integral asepsis.The factorial seedling growth of such as dendrobium candidum adopts the aseptic group of method trained mostly, if microbial infection, can cause rotting of tissue.In the amino acid whose culture experiment of research plant absorption, because amino acid can decompose rapidly under the effect of microorganism, then need the environment of integral asepsis.When measuring the transportion and transformation of some organic compound in soil, for avoiding the decomposition of microorganism, the activity of microorganism also to be suppressed.Therefore incubator and the Sterile culture method thereof that can keep gnotobasis for a long time is just needed.
In prior art, in long term open system, the easy device of aseptic culture also lacks relatively.Have a small amount of domestic and international laboratory can accomplish aseptic environment, but there is build maintenance cost higher, because of the large microorganism of area easily infect, operating personnel easily carry disease germs the problem entered.
Summary of the invention
The object of this invention is to provide a kind of simple and easy aseptic planting and train case and Sterile culture method thereof, by casing, fluorescent lamp, uviol lamp, first air cleaner, aspiration pump, culture tube, cultivate base, second air cleaner, high efficiency particle air filter, first syringe, 3rd air cleaner, water inlet pipe, outlet pipe, 4th air cleaner, second syringe composition, culture tube is inserted in be cultivated on base and is placed in casing, casing top is built-in is provided with fluorescent lamp, uviol lamp, casing side installs the second air cleaner and high efficiency particle air filter successively, opposite side installs the first air cleaner and aspiration pump successively, water inlet pipe one end is positioned at the top of culture tube, the other end of water inlet pipe through wall box successively with the 3rd air cleaner, first syringe connects, the bottom of culture tube connects outlet pipe, the other end of outlet pipe passes wall box and is connected with the 4th air cleaner and the second syringe successively.
In first air cleaner, the second air cleaner, the 3rd air cleaner, the 4th air cleaner, membrane aperture is 0.22 μm; high efficiency particle air filter adopts ultra-fine fibre glass paper to make filtrate; be mainly used in trapping the particle dust of below 0.5um and various suspension, prevent air particles from blocking the second air cleaner.
Another object of the present invention is to provide described simple and easy aseptic planting and trains the application of case in sterile nutrient solution cultivation plant seed.Realized by following steps:
1) plant seed is adopted ethanol, hydrogen peroxide, mercury chloride single or combination sterilizing, be positioned in the culture dish of autoclave sterilization and germinate;
2) casing is transferred on aseptic operating platform, open uviol lamp, to casing sterilizing 24h;
3) treat that seed root system grows into about 1.5cm, transfer them in the culture tube filling 0.5% agar, culture tube lid there is the aperture of diameter an about 0.5cm, transplant after 2 days, with the large 704 silicone rubber seal apertures in south, the effect of support and isolated microorganism can be played, transplant after 3-5 days, transferred in the culture tube that nutrient solution is housed by seed seedling and cultivate, aforesaid operations all carries out on aseptic operating platform, and all utensils used all adopt autoclave sterilization;
4) open aspiration pump, open fluorescent lamp, casing is transferred on clean table top;
5) after three days, adopt the second syringe by the sucking-off of culture tube Middle nutrition liquid, in culture tube, add new nutrient solution by the first syringe, afterwards according to plant growing way periodic replacement nutrient solution, as service time is long, air cleaner should be changed in time.
Another object of the present invention is to provide described simple and easy aseptic planting and trains the application of case in aseptic soil incubation plant seed.Realized by following steps:
1) plant seed is adopted ethanol, hydrogen peroxide, mercury chloride single or combination sterilizing, be positioned in the culture dish of autoclave sterilization and germinate;
2) casing is transferred on aseptic operating platform, open uviol lamp, to casing sterilizing 24h;
3) soil adopts 121 DEG C of sterilizing 30min, sterilizing is several times thoroughly to kill Soil Microorganism repeatedly, or adopt the patent No. to be that the soil sterilization device of ZL201420668133.1 carries out sterilizing, seed after vernalization is planted in the culture tube filling sterile soil, in soil, moisture is added by the first syringe, aforesaid operations all carries out on aseptic operating platform, and all utensils used all adopt autoclave sterilization;
4) open aspiration pump, open fluorescent lamp, casing is transferred on clean table top;
5) the first syringe is adopted to add moisture in soil according to soil dry-wet situation in incubation, avoid excessive addition of water to divide in incubation as far as possible, as need by soil moisture sucking-off, the second syringe then can be adopted excessive moisture sucking-off in culture tube, as long in service time (more than 25 days), should change air cleaner in time.
Described nutrient solution is Huo Gelan nutrient solution prescription, the rugged nutrient solution prescription in mountain or both modified formulas.
The present invention is reasonable in design, and make simple, cost is low, easy to operate, apparatus of the present invention can be applicable to earth culture and water planting two kinds of situations, effectively avoid microorganism to the change of composition in plant growth and culture matrix, and sterilization effect is good, can provide long-term gnotobasis for plant growth.
Accompanying drawing explanation
Fig. 1 is that simple and easy aseptic planting trains box structure schematic diagram.
Embodiment
The present invention is described further in conjunction with the accompanying drawings and embodiments.
embodiment 1
See Fig. 1, simple and easy aseptic training case of planting of the present invention is by casing 1, fluorescent lamp 2, uviol lamp 3, first air cleaner 4, aspiration pump 5, culture tube 6, cultivate base 7, second air cleaner 8, high efficiency particle air filter 9, first syringe 12, 3rd air cleaner 11, water inlet pipe 10, outlet pipe 13, 4th air cleaner 14, second syringe 15 forms, culture tube 6 is inserted in be cultivated on base 7 and is placed in casing 1, casing 1 top is built-in is provided with fluorescent lamp 2, uviol lamp 3, casing 1 side installs the second air cleaner 8 and high efficiency particle air filter 9 successively, opposite side installs the first air cleaner 4 and aspiration pump 5 successively, water inlet pipe 10 one end is positioned at the top of culture tube 6, the other end of water inlet pipe 10 through casing 1 sidewall successively with the 3rd air cleaner 11, first syringe 12 connects, the bottom of culture tube 6 connects outlet pipe 13, the other end of outlet pipe 13 passes casing 1 sidewall and is connected with the 4th air cleaner 14 and the second syringe 15 successively.
In first air cleaner 4, second air cleaner 8, the 3rd air cleaner 11, the 4th air cleaner 14, membrane aperture is 0.22 μm; high efficiency particle air filter 9 adopts ultra-fine fibre glass paper to make filtrate; be mainly used in trapping the particle dust of below 0.5um and various suspension, prevent air particles from blocking the second air cleaner 8.
embodiment 2a kind of aseptic soil cultural method
(1) plant seed is adopted routinely ethanol, hydrogen peroxide, mercury chloride single or combination sterilizing, be positioned in the culture dish of autoclave sterilization and germinate;
(2) simple and easy sterile board (Fig. 1) is transferred on aseptic operating platform, open uviol lamp 3, to casing sterilizing 24h;
(3) soil adopts 121 DEG C of sterilizing 30min, sterilizing is several times thoroughly to kill Soil Microorganism repeatedly, or adopt the patent No. to be that the soil sterilization device of ZL201420668133.1 carries out soil sterilization, being positioned over by seed after vernalization fills in the culture tube 6 of sterile soil, in soil, moisture is added by the first syringe 12, the 3rd air cleaner 11, water inlet pipe 10, aforesaid operations all carries out on aseptic operating platform, and all utensils used all adopt autoclave sterilization;
(4) open aspiration pump 5, open fluorescent lamp 2, casing 1 is transferred on clean table top;
(5) the first syringe 12 is adopted to add moisture in soil according to soil dry-wet situation in incubation, avoid excessive addition of water to divide in incubation as far as possible, as need by soil moisture sucking-off, the second syringe 15 then can be adopted excessive moisture sucking-off in culture tube 6, if service time is more than 25d, each air cleaner (the first air cleaner 4, second air cleaner 8, the 3rd air cleaner 11, the 4th air cleaner 14) should be changed in time.
embodiment 3a kind of sterile nutrient solution cultural method
(1) plant seed is adopted according to a conventional method ethanol, hydrogen peroxide, mercury chloride single or combination sterilizing, be positioned in the culture dish of autoclave sterilization and germinate;
(2) by simple and easy aseptic plant training case (Fig. 1) transfer on aseptic operating platform, open uviol lamp 3, to box house sterilizing 24h;
(3) treat that seed root system grows into about 1.5cm, transfer them in the culture tube 6 filling 0.5% agar (percent by volume), culture tube 6 lid (not shown) is provided with the aperture of diameter an about 0.5cm, transplant after 2 days, with the large 704 silicone rubber seal apertures in south, the effect of support and isolated microorganism can be played, transplant after 3-5 days, again seedling is transferred in the culture tube 6 that Huo Gelan nutrient solution is housed and cultivate, aforesaid operations all carries out on aseptic operating platform, and all utensils used all adopt autoclave sterilization;
(4) open aspiration pump 5, open fluorescent lamp 2, simple and easy nothing is planted training case and transfer on clean table top;
After (5) 3 days, adopt the second syringe 15 by the sucking-off of culture tube 6 Middle nutrition liquid, in culture tube 6, new nutrient solution is added by the first syringe 12, afterwards according to plant growing way regularly (being about three days) replacing nutrient solution, if service time is more than 25d, the filter that should respectively ventilate in time (the first air cleaner 4, second air cleaner 8, the 3rd air cleaner 11, the 4th air cleaner 14).
Described nutrient solution is Huo Gelan nutrient solution prescription, the rugged nutrient solution prescription in mountain or both modified formulas.
embodiment 4a kind of sterile nutrient solution cultural method
1) a variety of Chinese cabbage seed adopts the combination sterilizing methods of 70% ethanol sterilizing 1min-10% hydrogen peroxide sterilization 5min-0.1M mercury chloride sterilizing 5min, is positioned over afterwards in the culture dish of autoclave sterilization and germinates;
2) casing 1 is transferred on aseptic operating platform, open uviol lamp 3, to casing 1 sterilizing 24h;
3) treat that seed root system grows into about 1.5cm, transfer them to and fill in the culture tube 6 of 0.5% agar, culture tube 6 lid there is the aperture of diameter an about 0.5cm, transplant after 1-2 days, with the large 704 silicone rubber seal apertures in south, the effect of support and isolated microorganism can be played, transplant after 3 days, transferred to by seedling in the culture tube 6 that nutrient solution is housed and cultivate, aforesaid operations all carries out on aseptic operating platform, and all utensils used all adopt autoclave sterilization;
4) open aspiration pump 5, open fluorescent lamp 2, casing 1 is transferred on clean table top;
5) after 3 days, adopt the second syringe 15 by the sucking-off of culture tube 6 Middle nutrition liquid, in culture tube 6, add new nutrient solution by the first syringe 12, within every 3 days afterwards, change nutrient solution, cultivate 25d;
6) on aseptic operating platform, beef extract-peptone is adopted to carry out inspection bacterium to culture tube 6 Middle nutrition liquid, concrete operations are for getting 10g beef extract-peptone in 1000ml flask, 121 DEG C of sterilizings 30 minutes in high-pressure sterilizing pot, when temperature is down to about 80 DEG C, on aseptic operating platform, the peptone of about 15ml is in the culture dish of 10cm in diameter, after complete cooled and solidified, baked wheaten cake sterilizing tweezers are adopted to dip nutrient solution and drip in culture dish, cultivate 10 days at 25 DEG C, observe colony growth situation.
Described nutrient solution is Huo Gelan nutrient solution prescription, the rugged nutrient solution prescription in mountain or both modified formulas.
Result, see table 1, can be found out according to inspection bacterium result, adopts this device and asepticly plants culture method, non-microbiological contamination in cultivation a variety of Chinese cabbage 25d.
embodiment 5a kind of aseptic soil cultural method
1) corn seed is adopted the combination sterilizing methods of 70% ethanol sterilizing 1min-10% hydrogen peroxide sterilization 5min-0.1M mercury chloride sterilizing 5min, be positioned over afterwards in the culture dish of autoclave sterilization and germinate;
2) simple and easy aseptic training case of planting is transferred on aseptic operating platform, open uviol lamp 3, to casing 1 sterilizing 24h;
3) soil adopts 121 DEG C of sterilizing 30min, sterilizing is several times thoroughly to kill Soil Microorganism repeatedly, or adopt the patent No. to be that the soil sterilization device of ZL201420668133.1 carries out soil sterilization, being positioned over by the seed germinateed normal after vernalization 2d fills in the culture tube 6 of sterile soil, in soil, sterile water is added by the first syringe 12, the 3rd air cleaner 11, water inlet pipe 10, aforesaid operations all carries out on aseptic operating platform, and all utensils used all adopt autoclave sterilization; In soil, sterile water is added by the first syringe 12 afterwards according to soil dry-wet situation;
4) open aspiration pump 5, open fluorescent lamp 2, casing 1 is transferred on clean table top;
5) a small amount of sterile water is added by the first syringe 12 to soil in culture tube 6 according to soil dry-wet situation after;
6) on aseptic operating platform, beef extract-peptone is adopted to carry out inspection bacterium to culture tube 6 Middle nutrition liquid.Concrete operations are for getting 10g beef extract-peptone in 1000ml flask, 121 DEG C of sterilizings 30 minutes in high-pressure sterilizing pot, when temperature is down to about 80 DEG C, on aseptic operating platform, the beef extract-peptone that thaws of about 15ml is in the culture dish of 10cm in diameter, after complete cooled and solidified, adopts baked wheaten cake sterilizing tweezers gripping trace soil to be placed in culture dish, cultivate 10 days at 25 DEG C, observe colony growth situation.
Result, see table 2, can be found out according to inspection bacterium result, adopts this device and asepticly plants culture method, non-microbiological contamination in cultivation corn 25d.
Claims (8)
1. simple and easyly asepticly plant training case for one kind, it is characterized in that, described planter box is made up of casing (1), fluorescent lamp (2), uviol lamp (3), the first filter (4), aspiration pump (5), culture tube (6), cultivation base (7), the second air cleaner (8), high efficiency particle air filter (9), the first syringe (12), the 3rd air cleaner (11), water inlet pipe (10), outlet pipe (13), the 4th air cleaner (14), the second syringe (15), casing (1) top is provided with fluorescent lamp (2), uviol lamp (3), culture tube (6) is inserted in cultivation base (7) and goes up and be placed in casing (1), casing (1) side installs the second air cleaner (8) and high efficiency particle air filter (9) successively, opposite side installs the first air cleaner (4) and aspiration pump (5) successively, water inlet pipe (10) one end is positioned at the top of culture tube (6), the other end of water inlet pipe (10) through casing 1 sidewall successively with the 3rd air cleaner (11), first syringe (12) connects, the bottom of culture tube (6) connects outlet pipe (13), the other end of outlet pipe (13) is connected with the 4th air cleaner (14) and the second syringe (15) successively through casing (1) sidewall.
2. one according to claim 1 is simple and easy asepticly plants training case, and it is characterized in that, the first air cleaner (4), the second air cleaner (8), the 3rd air cleaner (11), the 4th air cleaner (14) interior membrane aperture are 0.22 μm.
3. one according to claim 1 is simple and easy asepticly plants training case, it is characterized in that, high efficiency particle air filter (9) adopts ultra-fine fibre glass paper to make filtrate.
4. simple and easy aseptic planting trains the application of case in sterile nutrient solution cultivation plant seed.
5. application according to claim 4, is characterized in that, is realized by following steps:
(1) plant seed is adopted according to a conventional method ethanol, hydrogen peroxide, mercury chloride single or combination sterilizing, be positioned in the culture dish of autoclave sterilization and germinate;
(2) simple and easy aseptic training case of planting is transferred on aseptic operating platform, open uviol lamp (3), to casing (1) sterilizing 24h;
(3) treat that seed root system grows into 1.5cm, transfer them in the culture tube (6) filling 0.5% agar, culture tube lid there is the aperture of diameter an about 0.5cm, transplant after 2 days, with the large 704 silicone rubber seal apertures in south, transplant after 3-5 days, seed seedling is transferred in the culture tube (6) that nutrient solution is housed and cultivate, aforesaid operations all carries out on aseptic operating platform, and all utensils used all adopt autoclave sterilization;
(4) opening aspiration pump (5), open fluorescent lamp (2), transferring on clean table top by planting training case;
After (5) three days, adopt the second syringe (15) by the sucking-off of culture tube (6) Middle nutrition liquid, in culture tube (6), new nutrient solution is added by the first syringe (12), afterwards according to plant growing way periodic replacement nutrient solution, if service time was more than 25 days, each air cleaner should be changed in time.
6. simple and easy aseptic planting trains the application of case in aseptic soil incubation plant seed.
7. application according to claim 6, is characterized in that, is realized by following steps:
(1) plant seed is adopted according to a conventional method ethanol, hydrogen peroxide, mercury chloride single or combination sterilizing, be positioned in the culture dish of autoclave sterilization and germinate;
(2) aseptic training case of planting is transferred on aseptic operating platform, open uviol lamp (3), to casing sterilizing 24h;
(3) soil adopts 121 DEG C of sterilizing 30min, or adopt the patent No. to be that the soil sterilization device of ZL201420668133.1 carries out soil sterilization, seed after vernalization is positioned in the culture tube (6) filling sterile soil, in soil, adds moisture by the first syringe; Aforesaid operations all carries out on aseptic operating platform, and all utensils used all adopt autoclave sterilization;
(4) open aspiration pump (5), open fluorescent lamp (2), casing 1 is transferred on clean table top;
(5) the first syringe (12) is adopted to add moisture in soil according to soil dry-wet situation in incubation, avoid excessive addition of water to divide in incubation as far as possible, as need by soil moisture sucking-off, then pass through the second syringe (15) by excessive moisture sucking-off in culture tube (6), if service time was more than 25 days, air cleaner should be changed in time.
8. application according to claim 5, is characterized in that, described nutrient solution is Huo Gelan nutrient solution prescription, the rugged nutrient solution prescription in mountain or both modified formulas.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510303082.1A CN104938333A (en) | 2015-06-07 | 2015-06-07 | Simple aseptic plant culture box and use thereof |
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| CN201510303082.1A CN104938333A (en) | 2015-06-07 | 2015-06-07 | Simple aseptic plant culture box and use thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638319A (en) * | 2016-03-31 | 2016-06-08 | 广州睿绅生物技术有限公司 | Dendrobium officinale seedling cultivation device and method |
| CN105675705A (en) * | 2016-02-25 | 2016-06-15 | 浙江大学 | Device and method for measuring moving rule of amino acid in soil |
| CN113331054A (en) * | 2021-05-31 | 2021-09-03 | 广西壮族自治区农业科学院 | Plant tissue culture method by adding culture medium under bacteria environment condition |
-
2015
- 2015-06-07 CN CN201510303082.1A patent/CN104938333A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105675705A (en) * | 2016-02-25 | 2016-06-15 | 浙江大学 | Device and method for measuring moving rule of amino acid in soil |
| CN105675705B (en) * | 2016-02-25 | 2018-12-25 | 浙江大学 | Measure the device and method of amino acid movement law in the soil |
| CN105638319A (en) * | 2016-03-31 | 2016-06-08 | 广州睿绅生物技术有限公司 | Dendrobium officinale seedling cultivation device and method |
| CN105638319B (en) * | 2016-03-31 | 2018-08-17 | 广州睿绅生物技术有限公司 | A kind of seedlings of Dendrobium officinale breeding apparatus and method |
| CN113331054A (en) * | 2021-05-31 | 2021-09-03 | 广西壮族自治区农业科学院 | Plant tissue culture method by adding culture medium under bacteria environment condition |
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