CN104928197A - Yeast fermenting medium and application thereof - Google Patents
Yeast fermenting medium and application thereof Download PDFInfo
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Abstract
The invention discloses yeast fermenting medium. The yeast fermenting medium comprises a carbon source, a nitrogen source and inorganic salt. The yeast fermenting medium is characterized by further comprising one or two of compounds in formula (1), wherein R stands for C1 to C20 alkyl groups. The invention further discloses a method for preparing yeast by the yeast fermenting medium. The method includes inoculating thallus into the sterilized yeast fermenting medium, aerating and culturing at 30 degrees Centigrade; controlling dissolved oxygen at 23+/-2%, liquidly adding 80% of glucose concentrated solution in the whole process until 1.5 hours before fermenting stops, controlling residual glucose of fermenting liquid below 0.2 g/dl, and finishing fermenting after 19 hours. By the yeast fermenting medium and application thereof, yeast yield and living cell rate of dry yeast can be remarkably increased, production cost is reduced and economic benefits are increased.
Description
Technical field
The present invention relates to a kind of yeast fermentation substratum and application thereof, particularly a kind ofly improve the yeast fermentation substratum of yeast yield and dry yeast living cell rate and preparing the application in yeast.
Background technology
Obtaining a large amount of yeast by liquid for aerobe fermentation is yeast series products (as active dry yeast, yeast extract) the industrial the first step.In the fermentation middle and later periods, in the thalline short period of time, oxygen requirement is very large.Now, be on the increase due to thalline and meta-bolites on the one hand and cause fermented liquid thickness, permeability of cell membrane reduces, and is unfavorable for the transmission of oxygen; The oxygen supply of fermentor tank reaches maximum at ferment middle mostly on the other hand, and fermenting process subsequently can not realize the increase of oxyty again by raising mixing speed and air quantity, yeast growth speed is reduced by the restriction of oxyty.And due to dissolved oxygen under-supply, impel yeast zymamsis, produce by product carbonic acid gas and ethanol.
Carbonic acid gas has restraining effect to yeast growth, also has impact to the form of cell.The generational loss of ethanol sugar, makes yeast yield reduce, production declining.Although the mode that employing control is sugared during the fermentation, it is also unpractical for controlling below the threshold concentration of ethanol generation by effectively sugared concentration in whole fermentative production.This is because: 1. sugared concentration is too low, and the growth of yeast is suppressed, and growth velocity will be very slow; 2., when sugared concentration is lower than 0.1g/L, general analytical procedure is adopted to measure more difficult.Ethanol is volatile substances, and in aerobic fermentation, a large amount of ventilations can take away the part ethanol in fermented liquid, and as when the ethanol content in fermented liquid is 2%, in exhaust, the content of ethanol is about 7g/m
3, thus affect the recovery rate of yeast, add production cost.
The yeast cell water content of state of nature is 70-80%, wherein Bound moisture content is 15-20%, free water content is 50-60%, certain loss of activity is had after the yeast cell of state of nature is made active dry yeast, and the degree of loss is relevant with the moisture content in yeast cell, in general, moisture is lower, more be conducive to the storage of active dry yeast, because the oxidized speed of yeast material reduces with the reduction of moisture content.But then, moisture content is lower, and in dry and reconstitution process, the loss of activity is larger.Moisture content high being unfavorable for preserves, and storage loss are large, and drying and rehydration are lost little.Therefore, the moisture content of active dry yeast must consider the stability of storage, considers loss of activity that is dry and reconstitution process again.
When moisture content is at 15-30%, there is no forfeiture due to yeast in conjunction with moisture, generally can not affect vigor and fermenting power, but the active dry yeast of this high-moisture can only refrigerate.When the moisture of active dry yeast is reduced to 15%, yeast has certain activity change, and the stability of at room temperature storing strengthens greatly, in drying and reconstitution process, has small portion loss of activity.When the moisture content of active dry yeast is 7.5-8.3%, active dry yeast can be stored at normal temperatures, and preservation period can reach half a year more than, in drying and reconstitution process, part cytoactive will be had to lose.For not adding protectant active dry yeast, when moisture content lower than 7.5% time, the loss of activity in dry and reconstitution process will increase greatly, and therefore do not add protectant active dry yeast, its moisture content should not lower than 7.5%.For the protectant active dry yeast of interpolation, its moisture content can be down to 4-5%, and preservation period can reach more than 1 year.
Cell yield in raising fermenting process and the living cell rate of stem cell have just become the Main Topics of zymotechnique.
Such as: Chinese patent CN102168016A discloses the agent of a kind of active dry yeast dehydration protection, specifically adopts glyceryl monostearate, sorbitan mono-oleic acid ester and glycerine.In addition, Chinese patent CN101045903 discloses the freeze-dry process of a kind of probiotic bacterium, effectively can preserve the probiotic bacteriums such as yeast.But aforesaid method protective material is in the yeast after joining fermentation, to fermentation for this significant process of yeast then without any measure of control.
Japanese Patent JP2014117213 discloses a kind of method preparing dry yeast, needs the step such as microwave radiation and drying under reduced pressure.US Patent No. 3868307 discloses a kind of production method of yeast saccharomyces cerevisiae, and the method adds mash and amylase in the medium.Aforesaid method can provide the yield of yeast cell maybe can provide yeast living cell rate, but aforesaid method or need microwave radiation device or add expensive zymin, production cost is higher.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of can improve yeast yield and cerevisin living cell rate and economy yeast fermentation substratum easily.
To achieve these goals, yeast fermentation substratum provided by the invention, comprises carbon source, nitrogenous source, inorganic salt, characterized by further comprising one in general formula (1) compound or two or more;
R represents C
1~ C
20alkyl.
Preferred general formula (1) compound R is C1 ~ C12 alkyl.Preferred compound is trimethyl-glycine, lauryl betaine (N-dodecyl-N, N-N-methylsarcosine), octyl group trimethyl-glycine (N-octyl group-N, N-N-methylsarcosine), sec.-propyl trimethyl-glycine (N-sec.-propyl-DMG).
General formula (1) compound role in the medium, may have following mechanism:
1, as efficient methyl donor, participate in methylation reaction and the enzyme work of key enzyme in pathways metabolism can be improved, accelerate biomass growth rate and metabolite output.
2, can permeability of cell membrane be improved, promote nutritive substance and the exchange of oxygen inside and outside born of the same parents, and improve the respiratory chain system of microorganism, significantly improve the respiratory characteristic of thalline.
3, as osmoprotectant, intraor extracellular osmotic pressure can be regulated, more be conducive to intracellular product to the release outside born of the same parents, solve and suppress problem by the too high feedback caused of Fermentation Substance Concentration in born of the same parents.Reduce the respiration inhibition that high osmotic pressure causes, accelerate oxygen in the transfer rate of intraor extracellular, reduce energy consumption.
4, there is as tensio-active agent the effect of emulsifying agent, more accelerate the transmission speed of oxygen in the fermented liquid of thickness.
Optimum general formula (1) compound R is methyl, is trimethyl-glycine, can be selected from one in BETAINE anhydrous, a water trimethyl-glycine, hydrochloride trimethyl-glycine, phosphoric acid betaine or two or more.
Trimethyl-glycine, has another name called N, N, Betaine, is a kind of quaternary ammonium type water-soluble alkaloid.Extract and trimethyl-glycine of gaining the name from beet because of initial, sterling is white flaky crystals, pleasantly sweet, the easy moisture absorption, quickly dissolving in water, easy digested absorption, use safety, has no side effect.Except the above-mentioned possible mechanism of action, trimethyl-glycine also has obvious beneficial effect to the dispersity and biomass that improve bacterium ball in seed liquor.
Concentration 0.015 ~ the 7.0g/dl of general formula (1) compound in yeast fermentation substratum, is preferably 0.05 ~ 0.5g/dl.
The special requirement of the carbon source of yeast fermentation substratum, can be selected from one in carbohydrate, grease, organic acid, normal alkane or two or more, be preferably Semen Maydis powder.
The nitrogenous source of yeast fermentation substratum can be selected from one in soybean cake powder, groundnut meal, fish meal, dried silkworm chrysalis meal, yeast powder, corn steep liquor, urea, ammonium salt, nitrate or two or more;
Above-mentioned inorganic salt are one in phosphoric acid salt, magnesium sulfate, sylvite or two or more.
Another object of the present invention, for providing a kind of method adopting above-mentioned yeast fermentation medium preparing yeast, comprises the following steps:
Thalline is accessed the described yeast fermentation substratum after sterilizing;
Blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%;
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl;
Fermentation culture terminates for 19 hours.
In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5 ~ 5.5.
The active dry yeast bacterium that the inventive method is suitable for comprises: bread yeast class, fodder yeast class, make wine uses yeast fungus class and medical yeast (such as: cloth Laplace yeast) class, nutritious yeast (such as: high-nucleic acid yeast, high iron yeast, containing selenium yeast with containing Zn-contained yeast) class etc.
The present invention, on prior art basis, reduces the generation of the anaerobic respiration in fermenting process, thus decreases the generation of ethanol and carbonic acid gas in fermenting process by adding general formula (1) compound in the fermentation medium; Eventually reduce suppression and infringement that carbonic acid gas produces yeast cell; The minimizing of ethanol growing amount, improve yeast to sugared yield and yeast output; Improve the ability of the resistance to infiltration of rear extraction stage yeast cell, the loss of material in block cell to a certain extent, thus reduce loss active in dry and reconstitution process, improve the viable bacteria rate of cerevisin, thus reduction production cost, increase economic efficiency.
Accompanying drawing explanation
Fig. 1 is alcohol concn trend comparison figure in embodiment 2,3,4 fermenting process
Fig. 2 is alcohol concn trend comparison figure in embodiment 2,5,6 fermenting process
Fig. 3 is alcohol concn trend comparison figure in embodiment 2,7,8 fermenting process
Fig. 4 is alcohol concn trend comparison figure in embodiment 2,9,10 fermenting process
Embodiment
Be described in further details the present invention below by embodiment, these embodiments are only used for the present invention is described, do not limit the scope of the invention.
Embodiment 1
First order seed is cultivated
Substratum (g/dl): glucose 3, peptone 2, yeast powder 0.1, adjustment pH to 5.5, at 115 DEG C, heats and carries out sterilizing in 20 minutes.
S. cervisiae CICC 1049 is inoculated in the 500mL shaking flask of 3 liquid amount 100mL, at 30 DEG C, after 18h is cultivated in 200r/min concussion, proceeds to secondary seed tank.
Secondary seed is cultivated
Substratum (g/dl): corn steep liquor 2, glucose 7, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.4.Adjustment pH to 5.0-5.5.3L substratum is put into the automatic fermenter of 5L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.First order seed is accessed secondary seed fermentor tank by inoculum size 10%, and blowing air is cultivated, and temperature is 30 DEG C.Cultivate and proceed to fermentor tank after 6 hours.
Embodiment 2
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 42.1%.Fermenting process detects alcohol concn (see Fig. 1) every sampling in 4 hours.Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 (SPAN 60) adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting living cell rate is 63%.
The mensuration of yeast dry weight (DCW, g): get 100ml fermented liquid centrifugal 10min under rotating speed 8000r/min, in 105 DEG C of drying in oven to constant weight, weigh; The measuring method of cell yield (%): the consumption (g/L) 100% of glucose in unit volume fermented yeast liquid dry cell weight (g/L)/unit volume fermented liquid; The measuring method of ethanol: potassium bichromate-ferrous sulfate volumetry; Mensuration (%) method of living cell rate: methylene blue staining.
Embodiment 3
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3, BETAINE anhydrous 0.15.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 44.8%.Fermenting process detects alcohol concn (see Fig. 1) every sampling in 4 hours.
Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, and obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting living cell rate is 75%.Other test conditionss, and yeast dry weight, cell yield, ethanol, living cell rate mensuration with embodiment 2.
Embodiment 4
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3, phosphoric acid betaine 0.15.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 44.2%.Fermenting process detects alcohol concn (see Fig. 1) every sampling in 4 hours.
Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, and obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting viable bacteria rate is 72%.Other test conditionss, and yeast dry weight, cell yield, ethanol, living cell rate mensuration with embodiment 2.
Embodiment 5
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3, lauryl betaine 0.02.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 45.0%.Fermenting process detects alcohol concn (see Fig. 2) every sampling in 4 hours.
Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, and obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting viable bacteria rate is 70%.Other test conditionss, and yeast dry weight, cell yield, ethanol, living cell rate mensuration with embodiment 2.
Embodiment 6
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3, octyl group trimethyl-glycine 0.7.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 44.9%.Fermenting process detects alcohol concn (see Fig. 2) every sampling in 4 hours.
Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, and obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting viable bacteria rate is 73%.Other test conditionss, and yeast dry weight, cell yield, ethanol, living cell rate mensuration with embodiment 2.
Embodiment 7
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3, sec.-propyl trimethyl-glycine 0.05.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 44.8%.Fermenting process detects alcohol concn (see Fig. 3) every sampling in 4 hours.
Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, and obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting viable bacteria rate is 71%.Other test conditionss, and yeast dry weight, cell yield, ethanol, living cell rate mensuration with embodiment 2.
Embodiment 8
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3, eicosyl trimethyl-glycine 0.5.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 44%.Fermenting process detects alcohol concn (see Fig. 3) every sampling in 4 hours.
Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, and obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting viable bacteria rate is 68%.Other test conditionss, and yeast dry weight, cell yield, ethanol, living cell rate mensuration with embodiment 2.
Embodiment 9
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3, hydrochloride trimethyl-glycine 0.05, empgen BB 0.05.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 45.1%.Fermenting process detects alcohol concn (see Fig. 4) every sampling in 4 hours.
Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, and obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting viable bacteria rate is 73%.Other test conditionss, and yeast dry weight, cell yield, ethanol, living cell rate mensuration with embodiment 2.
Embodiment 10
Fermentation culture
Fermention medium (g/dl) is: corn steep liquor 2.5, glucose 0.5, potassium primary phosphate 0.2, magnesium sulfate 0.05, ammonium sulfate 0.3, a water trimethyl-glycine 0.05, empgen BB 0.05, octyl group trimethyl-glycine 0.05.Adjustment pH to 5.0-5.5.9L substratum is put into the automatic fermenter of 15L, at 115 DEG C, heat and carry out sterilizing in 20 minutes.Fermentor tank cultivates by embodiment 1 secondary seed obtained by the inoculum size access of 10%, and blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%.
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl.In culturing process, adopt ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, make it maintain 4.5-5.5, fermentation culture terminates for 19 hours.Calculating cell yield is: 45.0%.Fermenting process detects alcohol concn (see Fig. 4) every sampling in 4 hours.
Fermented liquid physiological saline centrifuge washing 3 times, and the emulsifier span 60 adding 3wt% stablizes 15min, then centrifugal, granulation, low-temperature boiling is dry, and obtaining water content is the active dry yeast of 5.5%, and after vacuum places 2 weeks, detecting viable bacteria rate is 72%.Other test conditionss, and yeast dry weight, cell yield, ethanol, living cell rate mensuration with embodiment 2.
Find out (with embodiment 2 for comparative example) from each above embodiment, along with the generation adding the anaerobic respiration reduced in fermenting process of general formula (1) compound, thus decrease the generation of ethanol and carbonic acid gas in fermenting process; Eventually reduce suppression and infringement that carbonic acid gas produces yeast cell; The minimizing of ethanol growing amount, improves yeast to sugared yield and yeast output; Improve the ability of the resistance to infiltration of rear extraction stage yeast cell, the loss of material in block cell to a certain extent, thus reduce loss active in dry and reconstitution process, improve the viable bacteria rate of cerevisin, thus reduction production cost, increase economic efficiency.
Should be understood that; the above is only the preferred embodiment of the present invention, for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (10)
1. a yeast fermentation substratum, comprises carbon source, nitrogenous source, inorganic salt, characterized by further comprising one in general formula (1) compound or two or more;
R represents C
1~ C
20alkyl.
2. yeast fermentation substratum according to claim 1, is characterized in that described general formula (1) compound R is C
1~ C
12alkyl.
3. yeast fermentation substratum according to claim 1, is characterized in that described general formula (1) compound is trimethyl-glycine, lauryl betaine, octyl group trimethyl-glycine, sec.-propyl trimethyl-glycine.
4. yeast fermentation substratum according to claim 3, is characterized in that described general formula (1) compound is BETAINE anhydrous, a water trimethyl-glycine, hydrochloride trimethyl-glycine, phosphoric acid betaine.
5. yeast fermentation substratum according to claim 1, is characterized in that the concentration of described general formula (1) compound in described yeast fermentation substratum is 0.015 ~ 7.0g/dl.
6. yeast fermentation substratum according to claim 5, is characterized in that the concentration of described general formula (1) compound in described yeast fermentation substratum is 0.05 ~ 0.5g/dl.
7. yeast fermentation substratum according to claim 1, is characterized in that described carbon source is one in carbohydrate, grease, organic acid, normal alkane or two or more; Nitrogenous source is one in soybean cake powder, groundnut meal, fish meal, dried silkworm chrysalis meal, yeast powder, corn steep liquor, urea, ammonium salt, nitrate or two or more; Described inorganic salt are one in phosphoric acid salt, magnesium sulfate, sylvite or two or more.
8. the yeast fermentation substratum according to claim 1 ~ 7 any one is preparing the method in yeast, it is characterized in that comprising the following steps:
Thalline is accessed the described yeast fermentation substratum after sterilizing;
Blowing air is cultivated, and temperature is 30 DEG C.Control dissolved oxygen 23 ± 2%;
The glucose strong solution that whole process flowing adds 80% first 1.5 hours to fermentation ends, controls the amount of fermented liquid residual sugar at below 0.2g/dl;
Fermentation culture terminates for 19 hours.
9. the method prepared in yeast according to claim 8, is characterized in that in culturing process, adopts ammoniacal liquor and hydrochloric acid to regulate the pH of substratum, makes it maintain 4.5 ~ 5.5.
10. yeast fermentation substratum according to claim 9 is preparing the method in yeast, it is characterized in that described thalline is the one in bread yeast class, fodder yeast class, wine wine uses yeast fungus class and medical yeast class, nutritious yeast class.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400834A (en) * | 2016-01-08 | 2016-03-16 | 山东祥维斯生物科技股份有限公司 | Citric acid preparation method |
| CN105962360A (en) * | 2016-05-09 | 2016-09-28 | 山东祥维斯生物科技股份有限公司 | Method for producing microorganism ferment |
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| CN105400834A (en) * | 2016-01-08 | 2016-03-16 | 山东祥维斯生物科技股份有限公司 | Citric acid preparation method |
| CN105962360A (en) * | 2016-05-09 | 2016-09-28 | 山东祥维斯生物科技股份有限公司 | Method for producing microorganism ferment |
| CN107267337A (en) * | 2016-08-08 | 2017-10-20 | 山东祥维斯生物科技股份有限公司 | The preparation method of application and alcoholic beverage of the glycine betaine in alcoholic beverage is prepared |
| CN113151138A (en) * | 2021-03-25 | 2021-07-23 | 安徽华金味食品有限公司 | Yeast fermentation medium |
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