CN1974754A - Sugar cane juice and starch fermenting process for producing yeast - Google Patents
Sugar cane juice and starch fermenting process for producing yeast Download PDFInfo
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- CN1974754A CN1974754A CN 200610124204 CN200610124204A CN1974754A CN 1974754 A CN1974754 A CN 1974754A CN 200610124204 CN200610124204 CN 200610124204 CN 200610124204 A CN200610124204 A CN 200610124204A CN 1974754 A CN1974754 A CN 1974754A
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- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 53
- 240000000111 Saccharum officinarum Species 0.000 title claims abstract description 47
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims abstract description 47
- 235000011389 fruit/vegetable juice Nutrition 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 36
- 229920002472 Starch Polymers 0.000 title claims abstract description 10
- 235000019698 starch Nutrition 0.000 title abstract description 8
- 239000008107 starch Substances 0.000 title abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 26
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 22
- 239000011574 phosphorus Substances 0.000 claims abstract description 22
- 238000004519 manufacturing process Methods 0.000 claims abstract description 16
- 230000001954 sterilising effect Effects 0.000 claims abstract description 11
- 238000005352 clarification Methods 0.000 claims abstract description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 44
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 43
- 238000009423 ventilation Methods 0.000 claims description 23
- 229910021529 ammonia Inorganic materials 0.000 claims description 22
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 18
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 14
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 9
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 239000004202 carbamide Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 7
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 3
- 235000003534 Saccharomyces carlsbergensis Nutrition 0.000 claims description 3
- 241001123227 Saccharomyces pastorianus Species 0.000 claims description 3
- 244000017020 Ipomoea batatas Species 0.000 claims description 2
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 2
- 240000003183 Manihot esculenta Species 0.000 claims description 2
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 2
- 235000013312 flour Nutrition 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 150000003016 phosphoric acids Chemical class 0.000 claims description 2
- 229920001592 potato starch Polymers 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 229940100445 wheat starch Drugs 0.000 claims description 2
- 235000013379 molasses Nutrition 0.000 abstract description 19
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 16
- 239000002351 wastewater Substances 0.000 abstract description 12
- 239000002994 raw material Substances 0.000 abstract description 9
- 229910052799 carbon Inorganic materials 0.000 abstract description 8
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 8
- 210000005253 yeast cell Anatomy 0.000 abstract description 8
- 239000010865 sewage Substances 0.000 abstract description 4
- 235000016068 Berberis vulgaris Nutrition 0.000 abstract description 2
- 241000335053 Beta vulgaris Species 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000003344 environmental pollutant Substances 0.000 abstract 1
- 231100000719 pollutant Toxicity 0.000 abstract 1
- 238000000855 fermentation Methods 0.000 description 22
- 230000004151 fermentation Effects 0.000 description 22
- 238000003756 stirring Methods 0.000 description 21
- 238000005406 washing Methods 0.000 description 16
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 14
- 239000010786 composite waste Substances 0.000 description 8
- 239000008399 tap water Substances 0.000 description 8
- 235000020679 tap water Nutrition 0.000 description 8
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 7
- 229910019142 PO4 Inorganic materials 0.000 description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 230000010261 cell growth Effects 0.000 description 7
- 238000001035 drying Methods 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 238000010438 heat treatment Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 229910052760 oxygen Inorganic materials 0.000 description 7
- 239000001301 oxygen Substances 0.000 description 7
- 230000036284 oxygen consumption Effects 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 7
- 239000010452 phosphate Substances 0.000 description 7
- 229910052700 potassium Inorganic materials 0.000 description 7
- 239000011591 potassium Substances 0.000 description 7
- 238000004659 sterilization and disinfection Methods 0.000 description 7
- 208000028659 discharge Diseases 0.000 description 6
- 239000000049 pigment Substances 0.000 description 5
- 238000007599 discharging Methods 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- MIDXCONKKJTLDX-UHFFFAOYSA-N 3,5-dimethylcyclopentane-1,2-dione Chemical compound CC1CC(C)C(=O)C1=O MIDXCONKKJTLDX-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000013736 caramel Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000009923 sugaring Methods 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明涉及一种用甘蔗汁和淀粉发酵生产酵母的方法，包括：甘蔗榨汁后，经澄清处理，得到含糖11－17％的甘蔗汁；将得到的甘蔗汁加入发酵罐中，加入经灭菌处理的氮源和磷源；按5－20％的体积比种量接入酵母种液，通气酵拌，培养。本发明在酵母细胞培养过程中，联合使用甘蔗汁和淀粉两种原料作碳源和能源，实现高细胞密度培养，从而提高酵母的产率和质量。此外，本发明所提供的方法与传统的单纯利用甘蔗糖蜜或甜菜糖蜜原料生产酵母的方法相比，由于生产废水可部分循环回用，不仅可减少废水的排放量，而且废水的CODCr特别是色度等污染物的总量可大大降低，便于污水处理达标排放和实现清洁生产。The invention relates to a method for producing yeast by fermenting sugarcane juice and starch, comprising: after squeezing the sugarcane juice, performing clarification treatment to obtain sugarcane juice with a sugar content of 11-17%; adding the obtained sugarcane juice into a fermenter, adding Nitrogen source and phosphorus source for sterilizing treatment; according to the volume ratio of 5-20%, the seed quantity is inserted into the yeast seed liquid, aerated and fermented, and cultured. In the process of culturing yeast cells, the present invention uses sugarcane juice and starch as carbon sources and energy sources in combination to realize high cell density cultivation, thereby improving the yield and quality of yeast. In addition, the method provided by the present invention is compared with the traditional method of simply utilizing sugarcane molasses or beet molasses raw materials to produce yeast, because the production wastewater can be partially recycled, not only can reduce the discharge of wastewater, but also the COD Cr of wastewater is especially The total amount of pollutants such as chroma can be greatly reduced, which is convenient for sewage treatment to meet discharge standards and realize clean production.
Description
Technical field
The present invention relates to biotechnology, be specifically related to a kind of with sugar cane juice and amylofermentation production yeast method.
Technical background
The main raw material of China's yeast production is the by product of sugar industry a---waste molasses at present, utilize waste molasses to produce yeast and mainly have following problem: 1, in utilizing sugarcane or beet raw material sugaring process, the part sugar at high temperature is converted into caramel colorant, or and amino acid reaction generate the Mei Lade pigment, also has phenols pigment etc., not only cause the loss of fermentable sugar, and these molasses pigments also can suppress microorganism growth such as yeast, fermentation production rate is reduced greatly; 2, with the cane molasses be raw material, approximately produce 10-30m in the time of every production 1t dry yeast
3COD
CrUp to 50000-100000mg/L, and colourity reaches the fermenation raw liquid separation waste water of 3000-10000, also has COD
CrThe washing that reaches 10000-20000mg/L separates waste water 20-40m
3, also have pollutent generations such as the useless sediment of molasses pre-treatment in addition.It is estimated, the pollution load of the sanitary sewage that discharge the small city that waste water of producing the dry yeast factory discharging of 10000t per year is equivalent to 120,000 populations, and the wastewater treatment difficulty is big, expense is very high, has not only increased the yeast production cost greatly, and at present except adopting concentration method, the qualified discharge treatment technology that does not also have other practical, and concentration method energy consumption height not only, and the easy fouling of equipment stops up, and it is to be solved also to exist many problems to have.Being directed at the waste water that most of yeast producer discharged does not have qualified discharge even severe overweight, and pollution problem has become the bottleneck of restriction yeast manufacturing enterprise sustainable development.
Summary of the invention
The objective of the invention is to produce the problem that exists in the yeast, provide a kind of, reduce environmental pollution with sugar cane juice and amylofermentation production yeast method at existing waste molasses.
Of the present invention a kind of with sugar cane juice and amylofermentation production yeast method, comprise the steps:
(1) behind the sugar cane crushing,, obtains containing the sugar cane juice of sugared 11-17% through clarifying treatment;
(2) sugar cane juice that step (1) is obtained adds in the fermentor tank, adds nitrogenous source and phosphorus source through sterilising treatment;
(3) the kind amount (volume ratio) of pressing 5-20% inserts yeast kind liquid, and the ventilation ferment is mixed, and cultivates.
In the step (2), described nitrogenous source adopts urea, ammonium sulfate or liquefied ammonia; Phosphoric acid or phosphoric acid salt are adopted in described phosphorus source.
In the step (3), air flow be 0.3-2.0 litres of air/rise fermented liquid/minute.
In the step (3), described yeast kind is adopted with one or more mixtures in Rang brewer yeast (Saccharomyces cerevisiae Hansen), Ka Ersibai yeast (Saccharomyces carlsbergensis Hansen), the candiyeast (Candida utilis).
In the step (3), described cultivation adopts intermittent type, batch feeding or continuous mode to cultivate; Be batch feeding, intermittence or continuously stream add the sugar cane juice of mixing Dian Fentang that contains sugared 13-35% quality, wherein, the Dian Fentang consumption accounts for the 0-80% of total reducing sugar amount.
Described Dian Fentang is prepared through liquefaction, saccharification by tapioca (flour), W-Gum, sweet potato starch or wheat starch; Described sugar cane juice is obtained through squeezing or lixiviate, clarification by fresh cane.
In the step (2), added before the nitrogenous source and phosphorus source of sterilising treatment, can sterilize.
Adopt the present invention, the COD of factory effluent
CrEspecially colourity reduces greatly, far below molasses raw material waste water, but after suitably handling the part cyclically utilizing, about 60% the when wastewater flow rate of final discharging has only with molasses raw material, and sewage disposal is easy to qualified discharge and realizes cleaner production.
The present invention compared with prior art has following advantage:
1, directly is raw material, do not pass through the long high temperature evaporation enrichment step of sugar refinery process, avoided a large amount of generations of molasses pigments and the loss of fermentable sugar with the sugar cane juice;
2, avoided the generation of the too high inhibition yeast growth of molasses pigment, inorganic salt and heavy metal content phenomenon in the molasses, yeast production efficiency and yield are improved greatly;
3, greatly reduced the COD of waste discharge
CrAnd colourity, reuse capable of circulation after suitably handling, about 60% the when wastewater flow rate of final discharging has only with molasses raw material; Simultaneously, the waste water of discharging is easy to handle, and greatly reduces cost for wastewater treatment, and sewage disposal is easy to qualified discharge and realizes cleaner production.
Embodiment
Embodiment 1
The bacterial classification that adopts: yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), batch culture technological process
Sugarcane obtains containing the sugarcane mixing juice of sugar 14.1% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.5% fermentation culture base-material, get 3 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 300 milliliters of yeast saccharomyces cerevisiae kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control PH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.The highest Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 550 rev/mins, and fermentation later stage pH rises to 6.0～6.5, cultivated 9 hours, and centrifugal collection thalline, through washing drying, the yeast cell dry weight is 12.1 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 6150mg/L, colourity 80.
Embodiment 2
The bacterial classification that adopts: candiyeast (Candida utilis), batch culture technological process
Sugarcane obtains containing the sugarcane mixing juice of sugar 13.2% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.5% fermentation culture base-material, get 3 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, add independent heat sterilization nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and potassium primary phosphate), wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 150 milliliters of candiyeast kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.The highest Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 550 rev/mins, and fermentation later stage pH rises to about 6.4, cultivated 10 hours, and centrifugal collection thalline, through washing drying, the yeast cell dry weight is 12.6 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 6200mg/L, colourity 85.
Embodiment 3
The bacterial classification that adopts: Ka Ersibai yeast (Saccharomyces carlsbergensis Hansen), batch culture technological process
Sugarcane obtains containing the sugarcane mixing juice of sugar 14.3% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.5% fermentation culture base-material, get 3 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 200 milliliters of cereuisiae fermentum kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.The highest Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 520 rev/mins, and fermentation later stage PH rises to 6.5～6.7, cultivated 11 hours, and centrifugal collection thalline, through washing drying, the yeast cell dry weight is 11.6 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 6050mg/L, colourity 75.
Embodiment 4
The bacterial classification that adopts is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), batch feeding culture process process
Sugarcane obtains containing the sugarcane mixing juice of sugar 15.6% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.0% fermentation culture base-material, get 2.8 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 8 grams per liters and 4.5 grams per liters, insert 320 ml yeast kind liquid, carry out yeast culture.Initial Ventilation Rate is 1.6 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.Cultivate after 5 hours, divide the sugar cane juice of adding the starch-containing sugar of total reducing sugar 24.5% for three times (Dian Fentang account for total reducing sugar amount 60%), the highest Ventilation Rate and stirring velocity are respectively 5.2 liters/minute and 650 rev/mins, fermentation later stage pH rises to 6.3～6.5, it is 88 grams per liters that whole fermented liquid contains the total reducing sugar amount for 3.65 liters altogether, cultivates centrifugal collection thalline 12 hours, through washing drying, the yeast cell dry weight is 39.2 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 17200mg/L, colourity 230.
Embodiment 5
The bacterial classification that adopts is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), the continuous culture process process
Sugarcane obtains containing the sugar cane juice of sugar 14.5% after squeezing the juice, after the peace and quiet sugarcane juice of sulfurous method, add tap water and be deployed into and contain sugar 2.0% fermentation culture base-material, get 2.8 liters of rclarifying cane juices be added to 5 liters of bio-reactors (NewBrunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 280 ml yeast kind liquid, carry out yeast culture.Initial Ventilation Rate is 1.8 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.Cultivate after 5 hours, Continuous Flow adds the sugar cane juice that contains sugar 23.8% starch-containing sugar (Dian Fentang account for total reducing sugar amount 70%) and nitrogenous source, phosphorus source, and Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 600 rev/mins.When the fermentation liquid measure is 3.5 liters, enter the steady and continuous cultivation conditions, Continuous Flow adds the sugar cane juice that contains sugar 5.69% starch-containing sugar (Dian Fentang account for total reducing sugar amount 30%) and nitrogenous source, phosphorus source, and dilution rate (the unit volume substratum per hour sugar cane juice and the nutritive salt liquid of the starch-containing sugar that adds of stream is long-pending) is 0.2h
-1, cultured continuously 120 hours.Culturing process was taken a sample every 8 hours, centrifugal collection thalline, and through washing drying, the yeast cell dry weight is 26.2 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 12950mg/L, colourity 170.
Embodiment 6
The bacterial classification that adopts is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), the batch feeding culture process process of part waste water recycling
Sugarcane obtains containing the sugarcane mixing juice of sugar 13.3% after squeezing, after the peace and quiet sugarcane juice of sulfurous method, use embodiment 6 yeast separation and the resulting composite waste of washing to replace tap water to be deployed into the fermentation culture base-material that contains sugar 2.0%, get 2.8 liters of rclarifying cane juices be added to 5 liters of bio-reactors (New Brunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 280 milliliters of yeast saccharomyces cerevisiae kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 commentaries on classics part clocks, with 10% sulfuric acid and ammoniacal liquor control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.Cultivate after 5 hours, divide the sugar cane juice of adding the starch-containing sugar that contains sugar 24.1% for three times (Dian Fentang account for total reducing sugar amount 50%), the highest Ventilation Rate and stirring velocity are respectively 4.5 liters/minute and 650 rev/mins, it is 85 grams per liters that whole fermented liquid contains the total reducing sugar amount for 3.32 liters altogether, fermentation later stage pH rises to 6.2～6.4, cultivates centrifugal collection thalline 13 hours, through washing drying, the yeast cell dry weight is 34.1 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 18200mg/L, colourity 250.
Comparative Examples
The bacterial classification that adopts is yeast saccharomyces cerevisiae (Saccharomyces cerevisiae Hansen), uses cane molasses raw material batch feeding culture process process
Cane molasses adds tap water and is diluted to 30Bx, adds the vitriol oil and transfers pH3.8, is heated to 95 ℃ of insulations 5 minutes, leaves standstill 12 hours, must clarify molasses.Part is clarified molasses and is deployed into tap water and contains sugar 2.0% fermentation culture base-material, get 2.8 liters be added to 5 liters of bio-reactors (New Brunswick Scientific, USA) in.Sterilized 20 minutes for 115 ℃, be cooled to 35 ℃, the nitrogenous source (urea, ammonium sulfate, liquefied ammonia) and phosphorus source (phosphoric acid and the potassium primary phosphate) that add independent heat sterilization, wherein liquefied ammonia is without heating, make culture media nitrogen source and phosphorus source content be respectively 5 grams per liters and 3 grams per liters, insert 280 ml yeast kind liquid, carry out yeast culture.Initial Ventilation Rate is 2.0 liters/minute, and stir speed (S.S.) is 250 rev/mins, with 10% sulfuric acid and liquefied ammonia control pH4.5.30 ℃ of culture temperature.Along with the growth of cell, oxygen consumption rate increases gradually, in order to keep the fermentation dissolved oxygen level at 30-40%, suitably regulates Ventilation Rate and stirring velocity.Cultivate after 5 hours, divide the clarification liquid molasses of adding total reducing sugar 23.5% for three times, the highest Ventilation Rate and stirring velocity are respectively 5.0 liters/minute and 650 rev/mins, it is 80 grams per liters that whole fermented liquid contains the total reducing sugar amount for 3.45 liters altogether, fermentation later stage pH rises to about 6.0, cultivates centrifugal collection thalline 12 hours, through washing drying, the yeast cell dry weight is 32.5 grams per liters.Fermenation raw liquid separates the COD that separates composite waste with washing
CrBe 25860mg/L, colourity 5800.
Claims (6)
1, a kind of with sugar cane juice and amylofermentation production yeast method, it is characterized in that comprising the steps:
(1) behind the sugar cane crushing,, obtains containing the sugar cane juice of sugared 11-17% through clarifying treatment;
(2) sugar cane juice that step (1) is obtained adds in the fermentor tank, adds nitrogenous source and phosphorus source through sterilising treatment;
(3) the volume ratio kind amount of pressing 5-20% inserts yeast kind liquid, and the ventilation ferment is mixed, and cultivates.
2, method according to claim 1 is characterized in that in the step (2), and described nitrogenous source adopts urea, ammonium sulfate or liquefied ammonia; Phosphoric acid or phosphoric acid salt are adopted in described phosphorus source.
3, method according to claim 1 and 2 is characterized in that in the step (3), air flow be 0.3-2.0 litres of air/rise fermented liquid/minute.
4, method according to claim 3, it is characterized in that in the step (3) that described yeast kind is adopted with one or more mixtures in Rang brewer yeast (Saccharomyces cerevisiae Hansen), Ka Ersibai yeast (Saccharomycescarlsbergensis Hansen), the candiyeast (Candida utilis).
5, method according to claim 4 is characterized in that in the step (3), and described cultivation adopts intermittent type, batch feeding or continuous mode to cultivate; Be batch feeding, intermittence or continuously stream add the sugar cane juice of mixing Dian Fentang that contains sugared 13-35% quality, wherein, the Dian Fentang consumption accounts for the 0-80% quality of total reducing sugar amount.
6, method according to claim 5 is characterized in that described Dian Fentang is prepared through liquefaction, saccharification by tapioca (flour), W-Gum, sweet potato starch or wheat starch; Described sugar cane juice is obtained through squeezing or lixiviate, clarification by fresh cane.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101649277B (en) * | 2009-07-15 | 2013-01-30 | 刘名汉 | Foaming fruit/vegetable yellow wine and preparation method thereof |
| CN104560752A (en) * | 2014-12-26 | 2015-04-29 | 山东圣琪生物有限公司 | Technological method for producing high activity brewer's yeast from yeast waste water by using starch hydrolysis sugar as raw material |
| CN104928197A (en) * | 2015-06-25 | 2015-09-23 | 山东祥维斯生物科技有限公司 | Yeast fermenting medium and application thereof |
-
2006
- 2006-12-13 CN CN 200610124204 patent/CN1974754A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101649277B (en) * | 2009-07-15 | 2013-01-30 | 刘名汉 | Foaming fruit/vegetable yellow wine and preparation method thereof |
| CN104560752A (en) * | 2014-12-26 | 2015-04-29 | 山东圣琪生物有限公司 | Technological method for producing high activity brewer's yeast from yeast waste water by using starch hydrolysis sugar as raw material |
| CN104928197A (en) * | 2015-06-25 | 2015-09-23 | 山东祥维斯生物科技有限公司 | Yeast fermenting medium and application thereof |
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