CN104922165B - Peacilomyce hepiahi bacterium strain PH40 resist oxygen lack and it is antifatigue in purposes - Google Patents
Peacilomyce hepiahi bacterium strain PH40 resist oxygen lack and it is antifatigue in purposes Download PDFInfo
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- CN104922165B CN104922165B CN201510280984.8A CN201510280984A CN104922165B CN 104922165 B CN104922165 B CN 104922165B CN 201510280984 A CN201510280984 A CN 201510280984A CN 104922165 B CN104922165 B CN 104922165B
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Abstract
The present invention discloses a kind of peacilomyce hepiahi bacterium strain PH40Purposes in preparing resist oxygen lack and antifatigue medicine has apparent medical effect.With peacilomyce hepiahi bacterium strain PH40Bacterium powder is main component, is subject to a variety of pharmaceutic adjuvants, tablet, pulvis, capsule and the oral preparation being prepared.
Description
Technical field
The present invention discloses a kind of peacilomyce hepiahi bacterium strain PH40Resist oxygen lack and it is antifatigue in purposes, belong to Chinese medicine and
Field of health care products.
Background technology
Presentization city is lived in, the fast pace in city is experienced, people hold breathing, big to walk forward, to keep up with
In other people, to there is a steady operation, there are one happy families to make great efforts to gather around, and strives.And makes great efforts at us, strives
Meanwhile fatigue is also like the shadow following the person, constantly corrodes each " workaholic " body, let us body and mind is wan and sallow.In U.S. in 1987
Center for Disease Control of state just defines the concept of chronic fatigue syndrome, and one large-scale investigation of Britain in 1993 is shown：About 20%
Male and 25% women always feel fatigue；Investigation in 2001 ~ 2002 years then shows have 1.2 ten thousand people at least in 100,000 people
Continue 6 months confirmed fatigues；Japanese Department of Labor announces the Japanese whole nation in March, 2000 has 1/3 length of service population with chronic
Fatigue syndrome, fatigue, which has become today's society, influences a big key factor of our healths.
Oxygen is the key substance that human body carries out metabolism, is the primary need of human life activity, when anoxic a kind of phase
When common pathologic process leads to alveolar oxygen partial pressure and blood sample saturation degree since the insufficiency of intake of oxygen is to supply needed for body
It reducing, histocyte cannot obtain required oxygen from blood, can cause body dead to cause body injury, when serious,
Therefore, the research to anoxic is increasingly paid attention in the fields such as military affairs, sport, aerospace, underwater operation, tourism.
Paecilomyces hepiali chen is the endophyte being separated in natural cordyceps, is generally existing in natural cordyceps
A kind of cordyceps sinensis phorozoon, mycelium fermentation object have extensive pharmacological activity, can be used as natural cordyceps it is effective
The raw material of ingredient and effect, development prospect are wide.
Invention content
The present invention discloses a kind of peacilomyce hepiahi bacterium strain PH40In resist oxygen lack and antifatigue purposes, there is apparent doctor
Therapeutic effect.
Water extract of the present invention is can be used for the peacilomyce hepiahi bacterium strain PH of health food40Mycelium is raw material,
It is obtained using hot water extraction method, ensures the safety of medication.
Peacilomyce hepiahi bacterium strain PH40 of the present invention is with peacilomyce hepiahi bacterium strain（RCEF1429）For original bacteria
Strain, obtains, the bacterial strain is in preservation on December 26 in 2014 through mutagenesis screening《China typical culture collection center》, protect
Tibetan number is：CCTCC No：M 2014670；It is named as：paecilomyces hepidl chenPH40 ;Paecilomyces hepiali chen
Bacterial strain PH40.
Peacilomyce hepiahi bacterium strain PH of the present invention40Water extract is realized through the following technical scheme.
1, peacilomyce hepiahi bacterium strain PH is weighed40Dry powder is lyophilized, according to mass volume ratio（g:ml）1:10~1：50 ratio
Deionized water is added in example, and 50~100 DEG C are extracted 2~5 hours, are repeated once, the supernatant that merging is extracted twice, after rotary evaporation
Freeze-drying, obtains peacilomyce hepiahi bacterium strain PH40Water extract dry powder.
2, peacilomyce hepiahi bacterium strain PH is taken40Water extract dry powder is added pharmaceutic adjuvant, peacilomyce hepiahi bacterium is prepared
Strain PH40Water extract preparation.
Peacilomyce hepiahi bacterium strain is proved using mice burden swimming experiment, treadmill experiment, tired turn-club test for the first time
PH40Water extract anti-fatigue active is tested first using sodium nitrite poisoning experiment, oxygen deficit tolerance experiment, acute cerebral ischemia anoxic
Secondary proof peacilomyce hepiahi bacterium strain PH40Water extract Substituted phenyl-lactic acid is peacilomyce hepiahi bacterium strain PH40Water extract is as anti-
The research and development of fatigue and anti-hypoxia medicament and health products provide foundation.
The positive effect of the present invention is：Provide peacilomyce hepiahi bacterium strain PH40Prepare antifatigue and anti-hypoxia medicament
And the purposes in health products drug, and there is apparent medical effect.
Specific implementation mode
In order to be best understood from the technology contents of the present invention, the present invention is carried out below by specific implementation case detailed
Thin description.
Embodiment 1
Take peacilomyce hepiahi bacterium strain PH40Mycelium 100g, by mass volume ratio（g：ml）1:20 ratio addition go from
Sub- water 2L, 60 DEG C of extraction 4h, repeat to extract primary, merge supernatant twice, 60 DEG C be concentrated under reduced pressure after be lyophilized extract is dry
Powder 1.
Embodiment 2
1, peacilomyce hepiahi bacterium strain PH is taken40Mycelium 100g, by mass volume ratio（g：ml）1:30 ratio addition is gone
Ionized water 3L, 80 DEG C of extraction 3.5h repeat extraction once, merge supernatant twice, are lyophilized to obtain extraction after 80 DEG C of reduced pressures
Object dry powder 2.
2, extract dry powder 2 is taken, the pharmaceutic adjuvants such as starch, magnesium stearate are added, peacilomyce hepiahi bacterium strain is prepared
PH40Water extract preparation 2.
Embodiment 3
1, peacilomyce hepiahi bacterium strain PH is taken40Mycelium 1000g, by mass volume ratio（g：ml）1:30 ratio addition is gone
Ionized water 30L, 60 DEG C of extraction 3.5h repeat extraction once, merge supernatant twice, are lyophilized to obtain extraction after 80 DEG C of reduced pressures
Object dry powder 3；
2, extract dry powder 3 is taken, the pharmaceutic adjuvants such as sodium bicarbonate, sodium carboxymethylcellulose are added, bat moth is prepared
Paecilomyces varioti bacterial strain PH40Water extract preparation 3.
Following tests shows the present invention in purposes antifatigue, in anti-hypoxia health product
1, material and method
1.1 tested material embodiment, 1 dry powder 1,2 dry powder 2 of embodiment, 3 preparation 3 of embodiment.
1.2 experimental animal
Healthy Kunming kind female small white mouse 240, weight 18-22g are provided by Jilin University's Experimental Animal Center.Random point
It is 4 groups, every group 60.
1.3 zoopery room environmentals
22 DEG C of temperature, humidity 52%.
1.4 dosage select
The recommended dose of the health-care preparation is adult（It is calculated by weight 60kg）1.2g is right by 10 times of human body recommended amounts
Mouse is administered, and three groups of tested materials are configured to the solution of a concentration of 0.02g/ml, intragastric administration on mice administration with physiological saline respectively
Indices are measured after 30 days, gastric infusion volume is 0.1ml/10g.One blank control group is set simultaneously, with physiological saline generation
It is administered for tested material, gavage volume is identical as tested material.
1.5 experimental method
1.5.1 mouse fatigue turn-club test
Mouse is through gastric infusion peacilomyce hepiahi bacterium strain PH40After water extract preparation 30 days, in 30min after the last administration,
Each group mouse is individually placed on perspex bar, muscle is made to be in static(al) tense situation.Mouse fatigue turn-club test is carried out, it will
Transfer rod rotating speed is set as 15 revs/min, is trained to it, after each mouse falls 3 times on transfer rod, start recording mouse by
In the time that muscular fatigue is fallen from glass bar, parallel 3 times.Record the mouse fatigue transfer rod time.Highest mouse transfer rod is set
Time is 300 seconds.Experimental result is as shown in table 1.1.
The 1.1 mouse fatigue transfer rod time of table
As shown above, mouse is through gastric infusion Paecilomyces hepiali chen PH40After preparation, mouse fatigue can be significantly improved and turned
The stick time enhances the mouse limb coordination ability, as a result p<0.005, there is significant difference.
1.5.2 Endurance runs experiment
Mouse is through gastric infusion peacilomyce hepiahi bacterium strain PH40After water extract preparation 30 days, Endurance experiment is carried out, it will
Mouse is placed in FT-200 animal treadmills.Animal is first placed, 2min is trained to mouse, its understanding is made to flee from charging zone
Afterwards, rotating speed is set as 20rpm, electric current is turned up full blast, and the number that mouse is electrically shocked in 5min is recorded.As a result as shown in table 1.2.
1.2 Endurance of table runs number of shocks
The results show that peacilomyce hepiahi bacterium strain PH40Water extract preparation can significantly improve the mouse running time, reduce small
Mouse number of shocks, as a result p<0.005, there is significant difference.
1.5.3 mice burden swimming is tested
Mouse is through gastric infusion peacilomyce hepiahi bacterium strain PH40After water extract preparation 30 days, it is real to carry out mice burden swimming
It tests, mouse is placed in swimming with a load attached to the body tester, every group of system of rat-tail portion is put into 27 ± 1 DEG C of water temperature, water with the load of weight 10%
Depth is in the barrel type swimming pool of 3 times of bodies of mouse length（d=50cm）, measurement each group mouse continues swimming time and power exhausts the time.As a result
As shown in table 1.3.
The 1.3 mice burden swimming time of table
The results show that peacilomyce hepiahi bacterium strain PH40Water extract preparation can significantly improve the mice burden swimming time, prolong
Long mouse power exhausts the time, as a result p<0.005, there is significant difference.
1.5.4 mouse resist oxygen lack is tested
1.5.4.1 mouse oxygen deficit tolerance is tested
Mouse is through gastric infusion peacilomyce hepiahi bacterium strain PH40After water extract preparation 30 days, oxygen deficit tolerance experiment is carried out,
10g soda limes are put in 125ml wide-mouth bottles.Mouse is put into wide-mouth bottle, beyond the Great Wall rubber plug, is closed using vaseline.Mouse is measured to deposit
Live time.As a result as shown in table 1.4.
The 1.4 mouse oxygen deficit tolerance time-to-live of table
Experimental result shows that peacilomyce hepiahi bacterium strain water extract preparation can significantly improve mouse under the conditions of normobaric hypoxia
Death time, as a result p<0.005, there is statistical significance.
1.5.4.2 sodium nitrite poisoning experiment
Mouse is after gastric infusion peacilomyce hepiahi bacterium strain water extract preparation 30 days, and after last gavage 1h, each group is dynamic
Sodium nitrite 240mg/kgbw is injected intraperitoneally in object, clocks immediately, records the time-to-live of each test mice.As a result such as table 1.5
It is shown.
The 1.5 sodium nitrite poisoning mice time-to-live of table
Experimental result shows that it is dead that peacilomyce hepiahi bacterium strain water extract preparation can significantly improve sodium nitrite poisoning mice
It dies the time, as a result p<0.005, there is statistical significance.
1.5.4.3 acute cerebral ischemia anoxic is tested
Mouse is after gastric infusion peacilomyce hepiahi bacterium strain water extract preparation 30 days, after last gavage 1h, in second
Under ether light anaesthesia, quickly breaked end from mouse ears line with bulk pruning knife, to mouse diing time after breaking end by only record mouse.
As a result as shown in table 1.6.
The 1.6 acute cerebral ischemia anoxic experiment mice time-to-live of table
Experimental result shows that peacilomyce hepiahi bacterium strain water extract preparation can significantly improve the poisoning of acute cerebral ischemia anoxic
Mouse diing time, as a result p<0.005, there is statistical significance.
Claims (2)
1. purposes of the peacilomyce hepiahi bacterium strain PH40 in preparing resist oxygen lack and antifatigue medicine；The bacterial strain in
Preservation on December 26 in 2014《China typical culture collection center》, preserving number is：CCTCC M 2014670.
2. a kind of peacilomyce hepiahi bacterium strain PH40 water extracts, are prepared by the following method：
Using the freeze-drying dry powder of the peacilomyce hepiahi bacterium strain PH40 described in claim 1, according to mass volume ratio（g:ml）
1:10~1：Deionized water is added in 50 ratio, and 50~100 DEG C are extracted 2~5 hours, are repeated once, and merging is extracted upper twice
Clear liquid is lyophilized after rotary evaporation, obtains peacilomyce hepiahi bacterium strain PH40 water extract dry powder.
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| CN114098066A (en) * | 2021-11-25 | 2022-03-01 | 浙江百山祖生物科技有限公司 | Health food for relieving physical fatigue and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695509A (en) * | 2009-10-14 | 2010-04-21 | 香港理工大学深圳研究院 | Application of aweto cultured by artificial fermentation in preparing anti-fatigue and anti-hypoxia health product or drug |
| CN101991614A (en) * | 2010-09-13 | 2011-03-30 | 吴赣英 | Compound fermented cordyceps sinensis powder (paecilomyces hepiali Cs-4 bacterial powder) combined medicine and preparation thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695509A (en) * | 2009-10-14 | 2010-04-21 | 香港理工大学深圳研究院 | Application of aweto cultured by artificial fermentation in preparing anti-fatigue and anti-hypoxia health product or drug |
| CN101991614A (en) * | 2010-09-13 | 2011-03-30 | 吴赣英 | Compound fermented cordyceps sinensis powder (paecilomyces hepiali Cs-4 bacterial powder) combined medicine and preparation thereof |
Non-Patent Citations (1)
| Title |
|---|
| 蝙蝠蛾拟青霉菌丝体对小鼠体力疲劳及缺氧的影响;许宇辉等;《军事医学》;20141031;第38卷(第10期);第784-790页 * |
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