CN104914060A - 一种基于羊毛检测藏羊血清中锰含量的方法 - Google Patents
一种基于羊毛检测藏羊血清中锰含量的方法 Download PDFInfo
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Abstract
本发明公开了一种基于羊毛检测藏羊血清中锰含量的方法，包括如下步骤：1）采集羊毛 采集羊毛，洗洁精清洗干净，超纯水漂洗后，放入干燥箱烘干；2）测定羊毛中微量元素锰的含量 根据国家标准GB/T14924.12-2001中规定的实验动物微量元素锰的检测方法，采用原子吸收法检测羊毛中微量元素锰的含量；3）计算血清中微量元素锰的含量根据羊毛中微量元素锰与血清中锰的含量的相关性计算藏羊血清中锰的含量。本发明根据羊毛中锰的含量，计算出血液中锰的含量，进而确定该地区藏羊是否缺锰，可以做到针对性补饲，操作简单，方便快捷，避免了采血时对藏羊的伤害及应激。
Description
技术领域
本发明涉及一种基于羊毛检测藏羊血清中锰含量的方法。
背景技术
微量元素在动物体内的作用形式多种多样，构成生物体内一系列酶、蛋白质、激素、维生素等生物活性物质的重要组成部分，对机体代谢和生存有着重要作用。如果摄取不足、排泄过多或比列不当，均会引起生理功能或形态结构的异常。锰参与氧化还原反应、组织呼吸和骨骼的形成，影响动物生长、繁殖、血液形成和内分泌器官的功能。缺乏时骨骼发育异常和繁殖障碍。矿质元素引起的动物疾病临床症状有许多相似之处。如精神沉郁、食欲不振、消化障碍、生长发育停滞、贫血、生殖机能紊乱等，没有或缺乏特征性的临诊症状，容易与一般的营养不良、寄生虫病或一般中毒相混淆。因此，单纯的临诊学诊断都有局限性，必须借助检测手段，对环境、饲料、畜体等生物样品进行预防性检测，才有可能做到早期诊断。血液检验不仅是诊断各种血液病的主要依据，对其它系统疾病的诊断和鉴别也可提供许多重要信息，是临床医学检验中最常用的，最重要的基本内容。但是兽医临床上在进行动物采血时费时费力，由于应激等原因也容易给怀孕母畜造成流产，临床上许多牧民并不愿意给家畜进行采血。因此，建立方便、易行的检测方法非常必要。
发明内容
本发明要解决的技术问题是克服现有的缺陷，提供了一种基于羊毛锰含量法检测藏羊血液中锰含量的方法，该方法操作简便、针对性强、适用范围广泛。
本发明的目的通过以下技术方案来具体实现：
一种基于羊毛检测藏羊血清中锰含量的方法，包括如下步骤：
1）采集羊毛
采集羊毛，洗洁精清洗干净，超纯水漂洗后，放入干燥箱烘干；
2）测定羊毛中微量元素锰的含量
根据国家标准GB/T 14924.12-2001中规定的实验动物微量元素锰的检测方法，采用原子吸收法检测羊毛中微量元素锰的含量；
3）计算血清中微量元素锰的含量
根据羊毛中微量元素锰与血清中锰的含量的相关性计算藏羊血清中锰的含量。
作为优选方案，上述的基于羊毛检测藏羊血清中锰含量的方法，根据国家标准GB/T 14924.12-2001，采用原子吸收法分别测定羊毛、血清中微量元素锰的含量，找到两者的线性关系为： Y=0.366–0.014X，R2=0.9502，式中Y是血清中微量元素锰的含量，X是羊毛中锰的含量。
本发明用于检测藏羊血清中锰的含量，根据羊毛中锰的含量，计算出血液中锰的含量，进而确定该地区藏羊是否缺锰，可以做到针对性补饲，操作简单，方便快捷，避免了采血时对藏羊的伤害及应激。
具体实施方式
以下对本发明的优选实施例进行说明，应当理解，此处所描述的优选实施例仅用于说明和解释本发明，并不用于限定本发明。
实施例1：
一种基于羊毛检测藏羊血清中锰含量的方法
1、采集羊毛
采集5g羊毛，洗洁精清洗干净，超纯水漂洗后，放入干燥箱烘干。后剪碎后，精确称取1g ，用于微量元素锰的测定。
2、测定羊毛中微量元素锰的含量
按照国家标准GB/T 14924.12-2001中规定的实验动物微量元素锰的检测方法，采用原子吸收法检测羊毛中微量元素锰的含量。结果得到藏羊羊毛中微量元素锰的含量为12.83 μg/g。
3、计算血清中微量元素锰的含量
根据线性回归方程：Y=0.366–0.014X，R2=0.9502，式中Y是血清中微量元素锰的含量，X是羊毛中锰的含量，将12.83 μg/g代入式中，得出血清中微量元素锰的含量为0.1864μg/g。
试验例：
1 材料与方法
1.1试验地点及供试羊群
选择2~3岁、体重25~35kg健康藏羊10只，雌雄各半，由甘肃省肃南县皇城镇皇城羊场提供。
1.2试验方法
试验羊颈静脉采血5mL，血液凝固后离心，分离血清，-20℃保存，用于测定血清中锰的含量；背部剪毛5~10g，洗洁精自来水清洗后，用超纯水漂水3次，后放入50℃干燥箱中烘烤24h，剪碎后备用。
1.3 样品中微量元素锰的测定
根据国家标准GB/T 14924.12-2001，采用原子吸收法测定羊毛、血清中微量元素锰的含量。另外根据线性回归方程Y=0.366–0.014X，R2=0.9502，式中Y是血清中微量元素锰的含量，X是羊毛中锰的含量，根据羊毛锰的检测结果另行计算出血清锰的含量，并与原子吸收洁检测结果进行比较。
1.4数据处理
采用SPSS17.0对所有数据进行统计处理。
2 结果与分析
样品中微量元素锰的含量
试验结果见表1。由表1可以看出，根据线性回归方程计算出的藏羊血清锰的含量结果稳定，与血清锰原子吸收实测结果并无显著差异（P>0.05）。
表1 藏羊羊毛、血清中微量元素锰的含量
3 结论与讨论
本试验结果表明，根据线性回归方程Y=0.366–0.014X（R2=0.9502，式中Y是血清中微量元素锰的含量，X是羊毛中锰的含量）计算出的藏羊血清锰的含量结果稳定、与原子吸收实测值并无显著差异，可用于实际测定。
以上所述仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (2)
1.一种基于羊毛检测藏羊血清中锰含量的方法，其特征在于：包括如下步骤：
1）采集羊毛
采集羊毛，洗洁精清洗干净，超纯水漂洗后，放入干燥箱烘干；
2）测定羊毛中微量元素锰的含量
根据国家标准GB/T 14924.12-2001中规定的实验动物微量元素锰的检测方法，采用原子吸收法检测羊毛中微量元素锰的含量；
3）计算血清中微量元素锰的含量
根据羊毛中微量元素锰与血清中锰的含量的相关性计算藏羊血清中锰的含量。
2.根据权利要求1所述的基于羊毛检测藏羊血清中锰含量的方法，其特征在于：根据国家标准GB/T 14924.12-2001，采用原子吸收法分别测定羊毛、血清中微量元素锰的含量，找到两者的线性关系为： Y=0.366–0.014X，R2=0.9502，式中Y是血清中微量元素锰的含量，X是羊毛中锰的含量。
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58740A (ja) * | 1981-06-26 | 1983-01-05 | Toshiba Corp | ガリウム砒素中の微量元素の定量法 |
| CN1847846A (zh) * | 2005-04-14 | 2006-10-18 | 威尔基因科技股份有限公司 | 由毛发检测进行身体状况评量的方法 |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58740A (ja) * | 1981-06-26 | 1983-01-05 | Toshiba Corp | ガリウム砒素中の微量元素の定量法 |
| CN1847846A (zh) * | 2005-04-14 | 2006-10-18 | 威尔基因科技股份有限公司 | 由毛发检测进行身体状况评量的方法 |
Non-Patent Citations (3)
| Title |
|---|
| HAKAN BIRICIK等: "Seasonal Changes of some Mineral Status in Mares", 《JOURNAL OF EQUINE VETERINARY SCIENCE》 * |
| 吴劲川等: "毛发中微量元素与性别、年龄的相关性分析", 《科技创新导报》 * |
| 张磊等: "富硒地区山羊组织中的硒含量及其对PHGPx表达的影响", 《西北农林科技大学学报》 * |
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Application publication date: 20150916 |