CN110702638A - 一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法 - Google Patents
一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法 Download PDFInfo
- Publication number
- CN110702638A CN110702638A CN201911130864.4A CN201911130864A CN110702638A CN 110702638 A CN110702638 A CN 110702638A CN 201911130864 A CN201911130864 A CN 201911130864A CN 110702638 A CN110702638 A CN 110702638A
- Authority
- CN
- China
- Prior art keywords
- urine
- subacute
- cattle
- acidosis
- calcium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000283690 Bos taurus Species 0.000 title claims abstract description 74
- 208000010444 Acidosis Diseases 0.000 title claims abstract description 55
- 230000007950 acidosis Effects 0.000 title claims abstract description 55
- 208000026545 acidosis disease Diseases 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 15
- 210000002700 urine Anatomy 0.000 title claims description 75
- 239000011575 calcium Substances 0.000 title claims description 38
- 239000000835 fiber Substances 0.000 title claims description 37
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 title claims description 35
- 229910052791 calcium Inorganic materials 0.000 title claims description 35
- OBMBUODDCOAJQP-UHFFFAOYSA-N 2-chloro-4-phenylquinoline Chemical compound C=12C=CC=CC2=NC(Cl)=CC=1C1=CC=CC=C1 OBMBUODDCOAJQP-UHFFFAOYSA-N 0.000 title claims description 12
- 210000004767 rumen Anatomy 0.000 claims abstract description 34
- 235000013365 dairy product Nutrition 0.000 claims abstract description 7
- 230000002378 acidificating effect Effects 0.000 claims description 15
- 239000003599 detergent Substances 0.000 claims description 12
- 230000002485 urinary effect Effects 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 9
- 230000002550 fecal effect Effects 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 6
- 239000012530 fluid Substances 0.000 claims description 5
- 238000010219 correlation analysis Methods 0.000 claims description 3
- 238000010832 independent-sample T-test Methods 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 2
- 238000007619 statistical method Methods 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 10
- 235000013336 milk Nutrition 0.000 abstract description 8
- 239000008267 milk Substances 0.000 abstract description 8
- 210000004080 milk Anatomy 0.000 abstract description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract description 6
- 239000004202 carbamide Substances 0.000 abstract description 6
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 abstract 10
- 239000008280 blood Substances 0.000 abstract 10
- 210000004369 blood Anatomy 0.000 abstract 10
- 235000021243 milk fat Nutrition 0.000 abstract 5
- 210000003608 fece Anatomy 0.000 description 7
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- 235000015278 beef Nutrition 0.000 description 4
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 3
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 244000144992 flock Species 0.000 description 3
- 229940116269 uric acid Drugs 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229940109239 creatinine Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 238000003333 near-infrared imaging Methods 0.000 description 2
- 210000000664 rectum Anatomy 0.000 description 2
- JBYXPOFIGCOSSB-UQGDGPGGSA-N rumenic acid Chemical compound CCCCCC\C=C/C=C/CCCCCCCC(O)=O JBYXPOFIGCOSSB-UQGDGPGGSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000013211 curve analysis Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 208000016097 disease of metabolism Diseases 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
- G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
- G16C20/70—Machine learning, data mining or chemometrics
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Medical Informatics (AREA)
- Theoretical Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Artificial Intelligence (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Data Mining & Analysis (AREA)
- Databases & Information Systems (AREA)
- Immunology (AREA)
- Evolutionary Computation (AREA)
- Biochemistry (AREA)
- Software Systems (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Evolutionary Biology (AREA)
- Bioethics (AREA)
- Epidemiology (AREA)
- Computing Systems (AREA)
- Public Health (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
本发明提供一种利用血ALT、血L乳酸、乳脂、乳尿素诊断奶牛亚急性瘤胃酸中毒的方法，所述血ALT、血L乳酸、乳脂、乳尿素对奶牛亚急性瘤胃酸中毒比较敏感，通过检测血ALT、血L乳酸、乳脂、乳尿素含量来诊断奶牛亚急性瘤胃酸中毒，明确血ALT、血L乳酸、乳脂、乳尿素含量与奶牛亚急性瘤胃酸中毒均呈极显著相关性。确定当血ALT<16U/L时诊断牛患有亚急性瘤胃酸中毒的敏感度100.00％，特异性57.14％；当血L乳酸>0.37mmol/L时诊断牛患有亚急性瘤胃酸中毒的敏感度75.00％，特异性90.48％；当乳脂<3.41％时诊断牛患有亚急性瘤胃酸中毒的敏感度50.00％，特异性100.00％；当乳尿素<0.02％时诊断牛患有亚急性瘤胃酸中毒的敏感度84.62％，特异性90.48％。
Description
技术领域：
本发明涉及一种诊断牛亚急性瘤胃酸中毒的方法，具体涉及一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法。
背景技术：
畜牧业作为农业农村经济的重要支柱产业之一，是实现“乡村振兴”和“脱贫攻坚”的重要抓手，关系到粮食安全、食品安全、节能减排、农民就业和国际贸易等农业农村农民的各个方面，是引领中国农业农村实现现代化和可持续发展的基础性和战略性需求。伴随我国奶牛及肉牛行业从传统零散的养殖模式转变为规模化、集约化、标准化养殖模式，以及高产育种选育推广，片面追求高产等因素，导致目前规模化牛场重要群发性普通病高发群发，给养殖业带来巨大经济损失。亚急性瘤胃酸中毒是泌乳期母牛及育肥期肉牛常见的营养代谢病，疾病导致母牛产奶量降低、肉牛育肥效益低下、死淘率增加、易于继发蹄叶炎等，给养牛业造成了严重的经济损失。目前亚急性瘤胃酸中毒诊断的金标准依然是穿刺瘤胃，检测瘤胃液pH，而上述诊断方法极易造成牛瘤胃损伤、造成牛群应激，不利于牛群疾病的群体检测，导致牛群亚急性瘤胃酸中毒诊断难的现状。本发明拟通过检测分析亚急性瘤胃酸中毒奶牛尿液及粪便成分特征，进而建立一种利用尿液和粪便指标诊断牛亚急性瘤胃酸中毒的方法。
发明内容：
本发明的目的是为了克服上述现有技术存在的不足之处，而提供一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法。
本发明所采用的技术方案是：一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法，其中尿钙、尿pH、粪便酸性洗涤纤维均与牛亚急性瘤胃酸中毒密切相关，应用尿成分检测试剂盒测定牛尿钙、尿pH，用近红外法分析仪检测粪便酸性洗涤纤维含量，统计学分析表明尿钙、尿pH、粪便酸性洗涤纤维与牛亚急性瘤胃酸中毒的发生呈极显著负相关(P<0.01)，当尿钙<0.71mmol/L、尿pH<8.35或粪酸性洗涤纤维<29.95％时，可诊断奶牛患有亚急性瘤胃酸中毒。
上述方案中实验动物数量，检测指标尿钙、尿pH值、粪便酸性洗涤纤维均具有统计学意义。对亚急性瘤胃酸中毒组与健康组牛实验数据进行独立样本T检验，结果表明亚急性瘤胃酸中毒组牛尿钙极显著的低于健康组(P<0.01)，尿pH值、粪便酸性洗涤纤维极显著的低于健康组(P<0.01)。对实验数据进行Person相关性分析发现尿钙、尿pH、粪便酸性洗涤纤维含量与亚急性瘤胃酸中毒均呈极显著负相关(P<0.01)。对实验数据进行ROC曲线预警分析，尿钙、尿pH、粪酸性洗涤纤维曲线下面积分别为0.826、0.974、0.965，表明尿中尿钙、尿pH、粪酸性洗涤纤维对牛亚急性瘤胃酸中毒具有较好的预警作用。当尿钙<0.71mmol/L时诊断牛患有亚急性瘤胃酸中毒的敏感度83.33％，特异性70.00％；当尿pH<8.35时诊断牛患有亚急性瘤胃酸中毒的敏感度87.50％，特异性95.00％；当粪中酸性洗涤纤维<29.95％时诊断牛患有亚急性瘤胃酸中毒的敏感度100.00％，特异性81.82％。
本发明具有的有益效果是：本发明基于上述背景通过对亚急性瘤胃酸中毒牛与健康牛尿钙、尿pH、粪酸性洗涤纤维含量进行Person相关性分析与ROC曲线分析，确定当尿钙<0.71mmol/L、尿pH<8.35或粪中酸性洗涤纤维<29.95％可诊断牛患有亚急性瘤胃酸中毒，此方法可以非侵入性的群体监测牛群亚急性瘤胃酸中毒发病情况，为疾病的防控提供有效的技术支持，有效减少牧场经济损失。
具体实施方式：
下面结合实验对本发明进一步说明：
实施例
动物实验：于黑龙江省某采用自由卧栏、TMR(Total Mixed Rations全混合日粮)饲喂、年产奶量11.5吨的集约化奶牛养殖场随机选取2～3胎次产后7-14天新西兰荷斯坦奶牛44头，依据采食后6h瘤胃液pH值将其分为将其分为亚急性瘤胃酸中毒组(5.2<pH<5.8)及健康组(pH>6.2，无其他任何症状)，其中健康组24头，亚急性瘤胃酸中毒组20头。采集实验奶牛尿液与粪便。
粪便：从直肠中收集新鲜粪便200g，样品主要用于检测干物质(105℃)、酸性洗涤纤维(ADF)、粗蛋白、粗脂肪、淀粉，所有检测指标均使用近红外法分析检测。
尿液：阴道按摩法收集奶牛排尿中间的尿液50ml，利用尿成分检测试剂盒检测尿Ca、肌酐(CREA)、尿素(BUN)、尿酸(UA)、尿蛋白定量、pH。
数据分析：所有数据应用IBM SPSS19.0软件进行统计分析，计量资料用均数以x±s表示,采用独立样本T检验分析组间均值差异显著性；采用Pearson直线相关性分析各指标与亚急性瘤胃酸中毒相关性，P<0.01为极显著相关；采用ROC曲线分析显著性指标的预警值，曲线下面积大于0.5表示指标具有预警意义，在0.7～0.9之间表示具有一般的预警作用，大于0.9可以作为金指标进行诊断。
实验结果：结果如表1所示，亚急性瘤胃酸中毒奶牛尿液Ca、pH极显著低于健康组，UA、UPRO水平显著高于健康组。结果如表2所示，亚急性瘤胃酸中毒奶牛粪便酸性洗涤纤维(ADF)极显著低于健康组，粗蛋白水平显著高于健康组。
表1健康与亚急性瘤胃酸中毒奶牛尿生化指标差异性比较
表2健康与亚急性瘤胃酸中毒奶牛粪便成分差异性比较
表3亚急性瘤胃酸奶牛显著相关性指标
采用Pearson直线相关性分析各指标与亚急性瘤胃酸中毒相关性，结果如表3所示，奶牛尿Ca、尿pH、粪ADF结果与亚急性瘤胃酸中毒存在极显著负相关(P<0.01)。
利用ROC曲线分析尿钙、尿pH、粪酸性洗涤纤维指标对牛亚急性瘤胃酸中毒的预警作用，结果如表4所示，尿钙、尿pH、粪酸性洗涤纤维曲线下面积分别为0.826、0.974、0.965。表明尿中尿钙、尿pH、粪酸性洗涤纤维对牛亚急性瘤胃酸中毒具有较好的预警作用。当尿钙小于0.71mmol/L时诊断牛患有亚急性瘤胃酸中毒的敏感度83.33％，特异性70.00％，当尿pH<8.35时诊断牛患有亚急性瘤胃酸中毒的敏感度87.50％，特异性95.00％，当粪中酸性洗涤纤维<29.95时诊断牛患有亚急性瘤胃酸中毒的敏感度100.00％，特异性81.82％。
表4尿钙、尿pH、粪酸性洗涤纤维诊断参数
效果实施例
于黑龙江省某集约化繁育肉牛场选取产后5-14天西门塔尔泌乳母牛10头。采食后6h瘤胃液检测pH值；直肠中收集新鲜粪便200g，检测酸性洗涤纤维；阴道按摩法收集中段尿液50ml测尿钙、尿pH。依据尿钙<0.71mmol/L、尿pH<8.35、粪中酸性洗涤纤维<29.95％标准诊断牛亚急性瘤胃酸中毒，依据瘤胃液pH值评价诊断效果。
结果如表5所示，实际发生亚急性瘤胃酸中奶牛2头，依据尿钙<0.71mmol/L指标检出4头，依据尿pH<8.35指标检出3头，依据粪中酸性洗涤纤维<29.95％指标检出4头。
表5亚急性瘤胃酸中毒实施案例
Claims (2)
1.一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法，其特征在于：尿钙、尿pH、粪便酸性洗涤纤维均与牛亚急性瘤胃酸中毒密切相关，应用尿成分检测试剂盒和近红外法分析仪测定牛尿钙、尿pH、粪便酸性洗涤纤维含量，利用统计学分析尿钙、尿pH、粪便酸性洗涤纤维呈极显著负相关，P<0.01；当尿钙<0.71mmol/L、尿pH<8.35或粪酸性洗涤纤维<29.95％时，可诊断奶牛患有亚急性瘤胃酸中毒。
2.根据权利要求1所述的利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法，其特征在于：对亚急性瘤胃酸中毒组与健康组牛实验数据进行独立样本T检验，结果表明亚急性瘤胃酸中毒组牛尿钙、尿pH、粪便酸性洗涤纤维极显著低于健康组牛；对实验数据进行Person相关性分析发现，尿钙、尿pH、粪便酸性洗涤纤维与牛瘤胃液pH水平呈极显著负相关，P<0.01；实验数据进行ROC曲线预警分析，尿钙、尿pH、粪酸性洗涤纤维曲线下面积分别为0.826、0.974、0.965，当尿钙<0.71mmol/L、尿pH<8.35或粪酸性洗涤纤维<29.95％时可诊断奶牛患有亚急性瘤胃酸中毒。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911130864.4A CN110702638A (zh) | 2019-11-18 | 2019-11-18 | 一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911130864.4A CN110702638A (zh) | 2019-11-18 | 2019-11-18 | 一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110702638A true CN110702638A (zh) | 2020-01-17 |
Family
ID=69207184
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911130864.4A Pending CN110702638A (zh) | 2019-11-18 | 2019-11-18 | 一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110702638A (zh) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114106989A (zh) * | 2021-11-18 | 2022-03-01 | 黑龙江八一农垦大学 | 一种瘤胃体外发酵装置及其方法 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102068454A (zh) * | 2011-01-13 | 2011-05-25 | 王之盛 | 一种预防瘤胃酸中毒的缓释调控剂及其制备方法 |
| EP2424527A1 (en) * | 2009-04-29 | 2012-03-07 | Willowcroft Pharm, LLC | Mast cell stabilizers to prevent or treat laminitis |
| CN106770103A (zh) * | 2016-12-06 | 2017-05-31 | 盐城师范学院 | 一种检测硫代硫酸根离子的方法 |
| CN107699617A (zh) * | 2017-11-20 | 2018-02-16 | 中南大学湘雅二医院 | 一种早期诊断脓毒血症引发急性肾损伤分子标记物miR‑452、试剂盒及应用 |
| JP2018117531A (ja) * | 2017-01-23 | 2018-08-02 | 日産合成工業株式会社 | ペレット飼料及びペレット飼料の製造方法 |
-
2019
- 2019-11-18 CN CN201911130864.4A patent/CN110702638A/zh active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2424527A1 (en) * | 2009-04-29 | 2012-03-07 | Willowcroft Pharm, LLC | Mast cell stabilizers to prevent or treat laminitis |
| CN102068454A (zh) * | 2011-01-13 | 2011-05-25 | 王之盛 | 一种预防瘤胃酸中毒的缓释调控剂及其制备方法 |
| CN106770103A (zh) * | 2016-12-06 | 2017-05-31 | 盐城师范学院 | 一种检测硫代硫酸根离子的方法 |
| JP2018117531A (ja) * | 2017-01-23 | 2018-08-02 | 日産合成工業株式会社 | ペレット飼料及びペレット飼料の製造方法 |
| CN107699617A (zh) * | 2017-11-20 | 2018-02-16 | 中南大学湘雅二医院 | 一种早期诊断脓毒血症引发急性肾损伤分子标记物miR‑452、试剂盒及应用 |
Non-Patent Citations (3)
| Title |
|---|
| ANNE METTE DANSCHER ET AL: "Indicators of induced subacute ruminal acidosis (SARA) in Danish Holstein cows", 《ACTA VET SCAND》 * |
| 杨成栋 等: "牛瘤胃酸中毒的血液学及生化指标变化", 《中国牛业科学》 * |
| 白云龙 等: "酸性尿液的泌乳奶牛血/尿临床病理学变化", 《黑龙江八一农垦大学学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114106989A (zh) * | 2021-11-18 | 2022-03-01 | 黑龙江八一农垦大学 | 一种瘤胃体外发酵装置及其方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Stefańska et al. | Prevalence and consequence of subacute ruminal acidosis in Polish dairy herds | |
| Raizman et al. | The association between Mycobacterium avium subsp. paratuberculosis fecal shedding or clinical Johne's disease and lactation performance on two Minnesota, USA dairy farms | |
| Mamun et al. | Biochemical analysis of blood of native cattle in the hilly area of Bangladesh | |
| Jalali et al. | Effect of forage quality on intake, chewing activity, faecal particle size distribution, and digestibility of neutral detergent fibre in sheep, goats, and llamas | |
| Rogers et al. | Milk production, nutrient digestion, and rate of digesta passage in dairy cows fed long or chopped alfalfa hay supplemented with sodium bicarbonate | |
| Gusterer et al. | Sensor technology to support herd health monitoring: Using rumination duration and activity measures as unspecific variables for the early detection of dairy cows with health deviations | |
| Đuričić et al. | Effects of dietary clinoptilolite supplementation on β-hydroxybutirate serum level and milk fat to protein ratio during early lactation in Holstein-Friesian cows | |
| CN110702638A (zh) | 一种利用尿钙、尿pH、粪便酸性洗涤纤维诊断牛亚急性瘤胃酸中毒的方法 | |
| Xiao et al. | The age at first consumption of forage in calves and its effect on growth and rumination in the short-and long-term | |
| Mandok et al. | Efficiency of use of metabolizable energy for body weight gain in pasture-based, nonlactating dairy cows | |
| VLČEK et al. | Changes of fat-to-protein ratio from start to the midlactation and the impact on milk yield | |
| SKÁLOVÁ et al. | Seed germination test as a potential pregnancy diagnosis method for domestic cattle. | |
| Górski et al. | Changes in the level of selected haematological and biochemical parameters in the blood of dairy cows in central-eastern Poland | |
| Hart et al. | Adaptation of early-weaned lambs to high-concentrate diets with three grain sources, with or without sodium bicarbonate | |
| Kupczyński et al. | The influence of condition on the metabolic profile of Czech Fleckvieh cows in the perinatal period | |
| Necula et al. | Nutritional and genetical factors influencing Nitrogen metabolism and excretion in dairy cows: A review | |
| Tsukahara et al. | Across and within breed differences in the relationship between packed cell volume and fecal egg count in growing meat goat and hair sheep males naturally and artificially infected with gastrointestinal nematodes | |
| Lessire et al. | Evaluation of the ruminal function of Belgian dairy cows suspected of subacute ruminal acidosis. | |
| Ali et al. | Subclinical ketosis: Prevalence and some risk factors in cross breed and imported breed dairy cows in Mosul, Iraq | |
| CN114252590A (zh) | 一种利用尿胆原、尿pH、血UREA预测奶牛亚临床酮病的方法 | |
| Ondrejková et al. | Monitoring of Ascaris suum in slaughter pigs during 2000–2009 in Slovakia | |
| Kerwin et al. | Effect of replacing a portion of inorganic chloride trace minerals with trace mineral amino acid complexes | |
| Earing | Comparison of digestive function in young and mature horses | |
| Al-saadi et al. | Some physiological factors influence somatic cell counts and milk contents associated with mastitis in Iraqi buffaloes | |
| Giurgiu et al. | Relationship between the evolution of the puerperium milk ketone bodies values and the reproduction parameters in dairy cattle. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |