CN104911234A - Method for efficiently liquefying high-concentration corn starch step by step - Google Patents
Method for efficiently liquefying high-concentration corn starch step by step Download PDFInfo
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- CN104911234A CN104911234A CN201510296585.0A CN201510296585A CN104911234A CN 104911234 A CN104911234 A CN 104911234A CN 201510296585 A CN201510296585 A CN 201510296585A CN 104911234 A CN104911234 A CN 104911234A
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Abstract
The invention belongs to the technical field of manufacturing and processing sugar through starch, and particularly relates to a method for efficiently liquefying high-concentration corn starch step by step. According to the method, the technology that a medium-temperature alpha-amylase and high-temperature-resistant alpha-amylase are used for liquefying high-concentration corn starch milk step by step is a core technology, and the technical difficulty in liquefying of a high-concentration corn starch biological enzyme method is eliminated. The DE value of a liquefied product of the method ranges from 10 to 28, and a feasible method is provided for the high-concentration corn starch saccharification process next step. The optimal reaction condition is obtained through analysis and optimization achieved through a single factor and response surface experiment. The use amount of the medium-temperature alpha-amylase is preferentially 12-14 u/g on the dry basis, and the use amount of the high-temperature-resistant alpha-amylase is preferentially 10-12 u/g on the dry basis
Description
Technical field
The invention belongs to starch refine dsugar processing technique field, be specifically related to a kind of method of efficient substep liquefaction high density W-Gum.
Background technology
β-amylose is the important source material of China's foodstuffs industry, is the useful supplement of people's current consumption sugar.China's corn resources enriches, and accounts for second place of the world, and the volume increase of corn provides basic substance for developing β-amylose.But the β-amylose price of China is higher than sucrose, the principal element that price is high is corn price factor on the one hand, and the more important thing is that China's corn deep processing only rests on the production basis of traditional simple technique, only has the quality adopting new and high technology could improve starchy carbohydrate product further, significantly reduce costs, the β-amylose of Cai Nengshi China has the leeway of development at home and abroad.So the production cost in order to reduce β-amylose, one of the most effective approach improves the starting point concentration of starch, reduces the original water content in starch milk, and solid content in saccharified liquid is increased, and required evaporated water reduces.And the vital step of β-amylose, the cost of direct relation product and quality are produced in the liquefaction of high density W-Gum.
When utilizing Controlled-enzymatic Hydrolysis technological development W-Gum sugar products, the viscosity that the maximum technical problem faced is corn starch pasting is very large, degree of liquefaction is too low, it is large to produce water consumption.Semen Maydis powder contains abundant starch, and with regard to starch composition, its amylose starch is higher.So W-Gum is used for pharmacy or makes dextrin glucose etc.Modern sugar industry generally adopts enzyme process to prepare β-amylose, and what it adopted is generally Thermostable α-Amylase, and its optimum hydrolysis temperature is at about 90 DEG C, in the process that W-Gum heats up, W-Gum can produce gelatinization phenomenon after reaching 70 DEG C, makes solution be paste, viscosity is large, stir difficulty, therefore current sugar industry sugaring concentration is very low, and product form is based on liquid state, it produces power big energy-consuming, water consumption is many, and accumulating difficulty, these seriously constrain the development of China's sugar industry.
The present invention breaks through this technical bottleneck of high density starch biological enzyme liquefaction, solve starting point concentration by 25 ~ 35%, bring up to more than 50%, heating in gelatinization process, because concentration height viscosity can be very large, be difficult to stir and mix, the uneven partial starch that causes that is heated is aging, hinders the problems such as next step saccharification react.The present invention is according on the basis of existing α-amylase reaction conditions, take the collaborative liquefaction process of substep, solve and directly liquefy high density W-Gum because starch water-swelling is in Heat Gelatinization, liquefaction process with Thermostable α-Amylase, the viscosity of starch paste can be excessive, be difficult to stir and mix, react very difficult, finally cause age of starch to hinder the carrying out of next step saccharification react.Solve only too low with mesophilicα-diastase liquefaction high density W-Gum reaction efficiency, most of starch does not also form the problem such as dextrin, polysaccharide, has a strong impact on next step reaction and carries out, affect the quality of product simultaneously.Adopt two kinds of enzyme substep collaborative liquefaction process liquefaction high density W-Gum effects better, cost is lower.Liquefaction substrate need not carry out pre-treatment, reduces production cost, produces provide strong support for factory's industrialization.
Technical superiority major embodiment of the present invention is with the liquefaction of two kinds of α-amylase enzymes substep for core technology, by the reaction conditions of single factor test and response surface analysis optimization the best, solves this technical bottleneck of high density starch biological enzyme liquefaction.
Summary of the invention
The present invention is to provide a kind of method of efficient substep liquefaction high density W-Gum, and optimizes the reaction conditions of this enzyme system, finally solves only by biological enzyme liquefaction high density W-Gum problem.
Technical scheme of the present invention is as follows:
A kind of method of efficient substep liquefaction high density corn starch milk, it is characterized in that, make initial mass concentration with the allotment of commercially available W-Gum and count the high density corn starch milk of 40% ~ 65% for reaction substrate with butt, size mixing through starch, the enzymolysis process of two kinds of α-amylases substep α-amylase systems, obtain high density corn starch milk liquefaction liquid.
In aforesaid method, described process of sizing mixing, that any one weighing in common commercially available W-Gum deionized water, pure water, distilled water is mixed with the high density corn starch milk that massfraction is 40% ~ 65%, make starch milk even, stable with vortex mixer or electric mixer, regulate the pH of starch milk between 5.0 ~ 6.0 with hydrochloric acid and sodium hydroxide;
In aforesaid method, warm amylase liquefaction process in described first time, add mesophilicα-diastase in butt in uniform starch milk of sizing mixing: 6 ~ 28U/g starch, concentration is 0 ~ 0.2% Calcium Chloride Powder Anhydrous, is the 15 ~ 30min that liquefies under the condition of 65 ~ 75 DEG C after mixing in liquefaction temperature;
In aforesaid method, described second time fire resistant alpha-diastase liquefaction process, be by first time mesophilicα-diastase post liquefaction liquefier add Thermostable α-Amylase again in butt: 8-30U/g starch, be the 14 ~ 25min that liquefies under the condition of 82 ~ 96 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value, can as next step Mashing process reaction substrate between 10 ~ 28.
In aforesaid method, be utilize mesophilicα-diastase and Thermostable α-Amylase two kinds of enzymes as α-amylase, optimize the optimum reaction condition of substep α-amylase system, can efficiently liquefy high density corn starch milk.
Further, the consumption of mesophilicα-diastase is preferably in butt 10-15u/g.The consumption of Thermostable α-Amylase is preferably in butt 8-14u/g.
Embodiment
Embodiment 1
The corn starch milk being configured to 50% massfraction with deionized water is reaction substrate, sizing mixing evenly with vortex mixer regulates pH to be 5.8, mesophilicα-diastase is added in butt: 12u/g starch in uniform starch milk of sizing mixing, concentration is 0.2% Calcium Chloride Powder Anhydrous, be the 23min that liquefies under the condition of 70 DEG C in liquefaction temperature after mixing, liquefier is added again Thermostable α-Amylase in butt: 12u/g starch, be the 19min that liquefies under the condition of 94 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value is 26.59.
Embodiment 2
The corn starch milk being configured to 55% massfraction with deionized water is reaction substrate, sizing mixing evenly with vortex mixer regulates pH to be 5.5, mesophilicα-diastase is added in butt: 14u/g starch in uniform starch milk of sizing mixing, concentration is 0.15% Calcium Chloride Powder Anhydrous, be the 23min that liquefies under the condition of 70 DEG C in liquefaction temperature after mixing, liquefier is added again Thermostable α-Amylase in butt: 10u/g starch, be the 20min that liquefies under the condition of 92 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value is 24.99.
Embodiment 3
The corn starch milk being configured to 60% massfraction with deionized water is reaction substrate, sizing mixing evenly with vortex mixer regulates pH to be 5.5, mesophilicα-diastase is added in butt: 14u/g starch in uniform starch milk of sizing mixing, concentration is 0.2% Calcium Chloride Powder Anhydrous, be the 24min that liquefies under the condition of 72 DEG C in liquefaction temperature after mixing, liquefier is added again Thermostable α-Amylase in butt: 12u/g starch, be the 20min that liquefies under the condition of 94 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value is 26.78.
Embodiment 4
The corn starch milk being configured to 50% massfraction with deionized water is reaction substrate, sizing mixing evenly with vortex mixer regulates pH to be 6, mesophilicα-diastase is added in butt: 13u/g starch in uniform starch milk of sizing mixing, concentration is 0.2% Calcium Chloride Powder Anhydrous, be the 22min that liquefies under the condition of 70 DEG C in liquefaction temperature after mixing, liquefier is added again Thermostable α-Amylase in butt: 12u/g starch, be the 18min that liquefies under the condition of 90 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value is 23.59.
Embodiment 5
The corn starch milk being configured to 50% massfraction with deionized water is reaction substrate, sizing mixing evenly with vortex mixer regulates pH to be 5.8, mesophilicα-diastase is added in butt: 14u/g starch in uniform starch milk of sizing mixing, concentration is 0.2% Calcium Chloride Powder Anhydrous, be the 20min that liquefies under the condition of 70 DEG C in liquefaction temperature after mixing, liquefier is added again Thermostable α-Amylase in butt: 12u/g starch, be the 19min that liquefies under the condition of 92 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value is 25.13.
Embodiment 6
The corn starch milk being configured to 50% massfraction with deionized water is reaction substrate, sizing mixing evenly with vortex mixer regulates pH to be 5.5, mesophilicα-diastase is added in butt: 12u/g starch in uniform starch milk of sizing mixing, concentration is 0.2% Calcium Chloride Powder Anhydrous, be the 20min that liquefies under the condition of 70 DEG C in liquefaction temperature after mixing, liquefier is added again Thermostable α-Amylase in butt: 12u/g starch, be the 18min that liquefies under the condition of 94 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value is 24.30.
Embodiment 7
The corn starch milk being configured to 50% massfraction with deionized water is reaction substrate, sizing mixing evenly with vortex mixer regulates pH to be 5.8, mesophilicα-diastase is added in butt: 10u/g starch in uniform starch milk of sizing mixing, concentration is 0.2% Calcium Chloride Powder Anhydrous, be the 18min that liquefies under the condition of 70 DEG C in liquefaction temperature after mixing, liquefier is added again Thermostable α-Amylase in butt: 10u/g starch, be the 15min that liquefies under the condition of 92 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value is 22.92.
Embodiment 8
The corn starch milk being configured to 55% massfraction with deionized water is reaction substrate, sizing mixing evenly with vortex mixer regulates pH to be 5.8, mesophilicα-diastase is added in butt: 12u/g starch in uniform starch milk of sizing mixing, concentration is 0.2% Calcium Chloride Powder Anhydrous, be the 18min that liquefies under the condition of 70 DEG C in liquefaction temperature after mixing, liquefier is added again Thermostable α-Amylase in butt: 12u/g starch, be the 16min that liquefies under the condition of 94 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value is 24.92.
Claims (4)
1. the method for an efficient substep liquefaction high density W-Gum, it is characterized in that, make initial mass concentration with the allotment of commercially available W-Gum and count the high density corn starch milk of 40% ~ 65% for reaction substrate with butt, size mixing through starch, the enzymolysis process of two kinds of α-amylases substep α-amylase systems, obtain high density corn starch milk liquefaction liquid;
1) process of sizing mixing described in, that any one weighing in common commercially available W-Gum deionized water, pure water, distilled water is mixed with the high density corn starch milk that massfraction is 40% ~ 65%, make starch milk even, stable with vortex mixer or electric mixer, regulate the pH of starch milk between 5.0 ~ 6.0 with hydrochloric acid and sodium hydroxide;
2) warm amylase liquefaction process in the first time described in, add mesophilicα-diastase in butt in uniform starch milk of sizing mixing: 6 ~ 28U/g starch, concentration is 0 ~ 0.2% Calcium Chloride Powder Anhydrous, is the 15 ~ 30min that liquefies under the condition of 65 ~ 75 DEG C after mixing in liquefaction temperature;
3) the second time fire resistant alpha-diastase liquefaction process described in, be by first time mesophilicα-diastase post liquefaction liquefier add Thermostable α-Amylase again in butt: 8-30U/g starch, be the 14 ~ 25min that liquefies under the condition of 82 ~ 96 DEG C in liquefaction temperature after mixing, obtain high density W-Gum liquefier, its DE value, can as next step Mashing process reaction substrate between 10 ~ 28.
2. the method for a kind of efficient substep liquefaction high density W-Gum as claimed in claim 1, it is characterized in that: utilize mesophilicα-diastase and Thermostable α-Amylase two kinds of enzymes as α-amylase, optimize the optimum reaction condition of substep α-amylase system, can efficiently liquefy high density corn starch milk.
3. the method for a kind of efficient substep liquefaction high density W-Gum as claimed in claim 1, is characterized in that: the consumption of mesophilicα-diastase is preferably in butt 10 ~ 15u/g.
4. the method for a kind of efficient substep liquefaction high density W-Gum as claimed in claim 1, is characterized in that: the consumption of Thermostable α-Amylase is preferably in butt 8 ~ 14u/g.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434792A (en) * | 2016-11-30 | 2017-02-22 | 无锡甜丰食品有限公司 | Production process of glucose syrup |
| CN109098033A (en) * | 2018-09-18 | 2018-12-28 | 南京林业大学 | A kind of corrugated paper sizing agent and its application |
| CN109694891A (en) * | 2019-02-01 | 2019-04-30 | 吉林中粮生化有限公司 | A method of utilizing high-concentration starch milk continuous production starch saccharification products |
| CN110004054A (en) * | 2019-04-17 | 2019-07-12 | 中粮集团有限公司 | Liquid-returner, starch jet cooking equipment, the method for starch liquefacation device and starch liquefacation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1858260A (en) * | 2006-05-30 | 2006-11-08 | 赵佃团 | Processing process for producing cerealose using corn as raw material |
| CN101684504A (en) * | 2008-09-26 | 2010-03-31 | 保龄宝生物股份有限公司 | Preparation method of superhigh maltose syrup |
| CN104293863A (en) * | 2014-09-30 | 2015-01-21 | 江南大学 | Pre-treatment method for promoting high concentration starch to liquefy |
| CN104328156A (en) * | 2014-10-27 | 2015-02-04 | 山东广博生物技术服务有限公司 | Method of liquefying high-concentration starch milk by enzymatic process |
-
2015
- 2015-06-02 CN CN201510296585.0A patent/CN104911234A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1858260A (en) * | 2006-05-30 | 2006-11-08 | 赵佃团 | Processing process for producing cerealose using corn as raw material |
| CN101684504A (en) * | 2008-09-26 | 2010-03-31 | 保龄宝生物股份有限公司 | Preparation method of superhigh maltose syrup |
| CN104293863A (en) * | 2014-09-30 | 2015-01-21 | 江南大学 | Pre-treatment method for promoting high concentration starch to liquefy |
| CN104328156A (en) * | 2014-10-27 | 2015-02-04 | 山东广博生物技术服务有限公司 | Method of liquefying high-concentration starch milk by enzymatic process |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434792A (en) * | 2016-11-30 | 2017-02-22 | 无锡甜丰食品有限公司 | Production process of glucose syrup |
| CN109098033A (en) * | 2018-09-18 | 2018-12-28 | 南京林业大学 | A kind of corrugated paper sizing agent and its application |
| CN109694891A (en) * | 2019-02-01 | 2019-04-30 | 吉林中粮生化有限公司 | A method of utilizing high-concentration starch milk continuous production starch saccharification products |
| US10808270B2 (en) | 2019-02-01 | 2020-10-20 | Cofco Limited | Method for continuously producing starch saccharification products by using high-concentration starch milk |
| CN110004054A (en) * | 2019-04-17 | 2019-07-12 | 中粮集团有限公司 | Liquid-returner, starch jet cooking equipment, the method for starch liquefacation device and starch liquefacation |
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