CN104328156A - Method of liquefying high-concentration starch milk by enzymatic process - Google Patents
Method of liquefying high-concentration starch milk by enzymatic process Download PDFInfo
- Publication number
- CN104328156A CN104328156A CN201410583587.3A CN201410583587A CN104328156A CN 104328156 A CN104328156 A CN 104328156A CN 201410583587 A CN201410583587 A CN 201410583587A CN 104328156 A CN104328156 A CN 104328156A
- Authority
- CN
- China
- Prior art keywords
- amylase
- starch
- starch milk
- pullulanase
- enzymolysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method of liquefying high-concentration starch milk by an enzymatic process. The method comprises the following steps: (1) adding water into starch and blending to prepare high-concentration starch milk; (2) adding alpha-amylase and pullulanase into the high-concentration starch milk and uniformly stirring to prepare enzyme-containing starch milk; (3) performing enzymolysis on the enzyme-containing starch milk to prepare a pre-enzymolysis liquid; (4) adding high-temperature-resistant a-amylase into the pre-enzymolysis liquid, spraying and liquefying, and then, performing enzymolysis to prepare a liquefied liquid. According to the method disclosed by the invention, the mass concentration of the starch milk is 40-50% which is remarkably increased in comparison with 20-30% in the prior art, so that the amounts of the production water and the vapor are greatly reduced, and therefore, the production cost is lowered by over 20%.
Description
Technical field
The present invention relates to a kind of method of enzymatic liquefaction high-concentration starch milk, belong to starch processing technique field.
Background technology
China is the second largest Maize Production state being only second to the U.S. in the world, produces corn 1.5 hundred million tons per year, except the corn that fodder industry consumption is a large amount of, is mainly used in the deep processing of corn.At present, China has defined a series of corn deep processing products such as W-Gum, alcohol, organic acid, β-amylose, and year processing corn reaches about 4,000,000 tons.The products such as corn deep processing alcohol, organic acid, β-amylose all need through starch liquefacation step, and just can carry out next step manufacturing procedure, therefore the liquefaction of starch is positioned at the key position of the whole industry of starch deep processing.
Chinese patent literature CN 101684504A (application number CN200810139488.0) discloses a kind of preparation method of superhigh maltose syrup, comprise and starch is sized mixing, high-temperature starch enzyme liquefaction, beta-amylase, Pullulanase, maltin three enzyme cooperative saccharification, and the exquisite technology such as ultrafiltration obtains superhigh maltose syrup.
Chinese patent literature CN1587421A (application number CN200410051025.0) discloses a kind of production method comprising the high purity malt sugar product of high purity maltose syrup, maltose alcohol and solid maltose, comprise: in starch milk, add high-temperatureα-amylase, through steam ejection liquefaction, cooling, add beta-amylase, Pullulanase and trisaccharide maltose lytic enzyme, synergetic hydrolysis, adds middle temperature amylase, obtains malt syrup after saccharification; Process obtains other goods on this basis.
But along with the development of starch deep processing industry, traditional liquefaction process is owing to needing a large amount of steam and water of productive use, and produce a large amount of sewage, cause serious problem of environmental pollution, cause the great attention of national correlation department, therefore transform traditional processing technology by new technology, make corn deep processing industry become resource-conserving, environmentally friendly enterprise is imperative.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of method of enzymatic liquefaction high-concentration starch milk is provided.Production method provided by the invention, by strengthening the concentration of starch milk, has greatly been saved steam consumption and water of productive use, has been decreased sewage discharge, had important promotional value.
In order to realize object of the present invention, technical solution of the present invention is as follows:
A method for enzymatic liquefaction high-concentration starch milk, comprises the steps:
(1) mass concentration adjusting starch milk that adds water in starch is 40 ~ 50%, adjusts pH 5.5 ~ 6.5, temperature 50 ~ 70 DEG C, obtained high-concentration starch milk;
(2) in the obtained high-concentration starch milk of step (1), a-amylase and Pullulanase is added, a-amylase add-on is that every gram of starch adds 4 ~ 10U, Pullulanase add-on is that every gram of starch adds 4 ~ 8ASPU, stirs, obtained enzyme-added starch milk;
(3) by enzyme-added starch milk enzymolysis 30 ~ 50min that step (2) is obtained, obtained pre-enzymolysis solution;
(4) in the obtained pre-enzymolysis solution of step (3), thermostableα-amylase is added, the add-on of thermostableα-amylase is that every gram of starch adds 18 ~ 25LU, 105 DEG C ~ 107 DEG C steam ejection liquefaction 5 ~ 10min, then at 95 DEG C ~ 102 DEG C enzymolysis 45 ~ 90min, obtained DE value is the liquefier of 10 ~ 15.
Preferred according to the present invention, in described step (1), the mass concentration of starch milk is 43 ~ 47%.
Preferred according to the present invention, in described step (2), a-amylase is middle temperature a-amylase.
Preferred according to the present invention, in described step (2), Pullulanase is the Pullulanase that Wuxi biotechnology company limited of Jie Neng section produces, and a-amylase is the middle temperature a-amylase that Wuxi biotechnology company limited of Jie Neng section produces.
Preferred according to the present invention, in described step (2), add a-amylase and Pullulanase carries out under agitation.
Preferred according to the present invention, in described step (2), a-amylase add-on is that every gram of starch adds 6 ~ 8U, and Pullulanase add-on is that every gram of starch adds 5 ~ 7ASPU.
Preferred according to the present invention, also comprise the step of carrying out ultrasonication in described step (3) enzymolysis process.
Further preferred, described ultrasonication condition is: power 3000 ~ 5000W, frequency: 20KHz, working hour 10 ~ 30S, interval time 10 ~ 20S, ultrasonic treatment time is identical with the enzymolysis time of step (3).
More excellent, described ultrasonication condition is: power 5000W, frequency: 20KHz, working hour 30S, interval time 10S.
Preferred further, described ultrasonication is for carry out under agitation.
Preferred according to the present invention, in described step (4), thermostableα-amylase is the efficient high-temperature resistant a ?amylase that Wuxi biotechnology company limited of Jie Neng section produces.
Beneficial effect
1, current conventional production process starch concentration is about 20%, and starch quality concentration of the present invention is 40 ~ 50%, and 20 ~ 30% of more existing routine techniques significantly improves, and can save water of productive use and steam consumption in a large number, reduces production cost and reaches more than 20%;
2, the present invention's warm a-amylase and Pullulanase combined pretreatment in creatively adopting, middle temperature a-amylase can α-l in hydrolyzed starch molecule, and 4 glucoside bonds, make the DE value of liquefier rise rapidly, the viscosity of starch paste is declined rapidly; Pullulanase promptly can cut α-1,6 glycosidic link in amylopectin tapping point, cuts whole branched structure, forms amylose starch, reduces rapidly the viscosity of starch paste; Thus solve the not liquescent technical barrier of high concentration starch milk;
3, the present invention is in the process of middle temperature a-amylase and Pullulanase combined pretreatment, ultrasonic wave is adopted to process feed liquid, ultrasonication can make starch particle surface become more coarse, there is depression, add the specific surface area of particle, increase and middle temperature a-amylase and Pullulanase contact area, thus make enzyme more obvious to the Degradation of starch granules in starch pasting, liquefaction process.
4, the present invention adopts efficient high-temperature resistant amylase to carry out steam ejection liquefaction, and efficient high-temperature resistant amylase in most of the cases, does not need to add calcium ion, decreases calcium ion to refining impact, also saves expense; And efficient high-temperature resistant amylase operates under lower pH condition, can not produce gentiobiose; In addition, efficient high-temperature resistant amylase at high temperature Heat stability is good, protein condenses is good, is convenient to protein separation and recycles.
Embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further elaborated, but institute of the present invention protection domain is not limited thereto.
Efficient high-temperature resistant a ?amylase, Wuxi biotechnology company limited of Jie Neng section is on sale, enzyme 17400LU/g alive;
Pullulanase, Wuxi biotechnology company limited of Jie Neng section is on sale, enzyme 1000ASPU/g alive;
Middle temperature α ?amylase, Wuxi biotechnology company limited of Jie Neng section is on sale, enzyme 6000u/g alive;
Ultrasonication instrument: Xin Dong and ultrasonic equipment manufactory on sale
Embodiment 1
A method for enzymatic liquefaction high-concentration starch milk, comprises the steps:
(1) mass concentration adjusting starch milk that adds water in starch is 40%, adjusts pH 5.5, temperature 50 C, obtained high-concentration starch milk;
(2) in the obtained high-concentration starch milk of step (1), a-amylase and Pullulanase is added, a-amylase add-on is that every gram of starch adds 4U, Pullulanase add-on is that every gram of starch adds 4ASPU, stirs, obtained enzyme-added starch milk;
(3) by the enzyme-added starch milk enzymolysis 30min under agitation that step (2) is obtained, process by ultrasonic wave, obtained pre-enzymolysis solution simultaneously;
Ultrasonication condition is: power 4000W, frequency: 20KHz, working hour 20S, interval time 20S;
After testing, pre-enzymolysis solution is 60 DEG C of viscosity numbers in temperature is 30mPas.
(4) in the obtained pre-enzymolysis solution of step (3), thermostableα-amylase is added, the add-on of thermostableα-amylase is that every gram of starch adds 18LU, 105 DEG C of DEG C of steam ejection liquefaction 5min, then at 95 DEG C of DEG C of enzymolysis 45min, obtained DE value is the liquefier of 10.
After testing, liquefier is 60 DEG C of viscosity numbers in temperature is 7mPas.
Embodiment 2
A method for enzymatic liquefaction high-concentration starch milk, comprises the steps:
(1) mass concentration adjusting starch milk that adds water in starch is 50%, adjusts pH 6.5, temperature 70 C, obtained high-concentration starch milk;
(2) in the obtained high-concentration starch milk of step (1), a-amylase and Pullulanase is added, a-amylase add-on is that every gram of starch adds 10U, Pullulanase add-on is that every gram of starch adds 8ASPU, stirs, obtained enzyme-added starch milk;
(3) by enzyme-added starch milk enzymolysis 30 ~ 50min under agitation that step (2) is obtained, process by ultrasonic wave, obtained pre-enzymolysis solution simultaneously;
Ultrasonication condition is: power 5000W, frequency: 20KHz, working hour 30S, interval time 10S;
After testing, pre-enzymolysis solution is 60 DEG C of viscosity numbers in temperature is 33mPas.
(4) in the obtained pre-enzymolysis solution of step (3), thermostableα-amylase is added, the add-on of thermostableα-amylase is that every gram of starch adds 25LU, 107 DEG C of steam ejection liquefaction 10min, then at 102 DEG C of enzymolysis 90min, obtained DE value is the liquefier of 15.
After testing, liquefier is 60 DEG C of viscosity numbers in temperature is 8mPas.
Embodiment 3
A method for enzymatic liquefaction high-concentration starch milk, comprises the steps:
(1) mass concentration adjusting starch milk that adds water in starch is 45%, adjusts pH 6.0, temperature 60 C, obtained high-concentration starch milk;
(2) in the obtained high-concentration starch milk of step (1), a-amylase and Pullulanase is added, a-amylase add-on is that every gram of starch adds 8 U, Pullulanase add-on is that every gram of starch adds 6 ASPU, stirs, obtained enzyme-added starch milk;
(3) by the enzyme-added starch milk enzymolysis 40min under agitation that step (2) is obtained, process by ultrasonic wave, obtained pre-enzymolysis solution simultaneously;
Ultrasonication condition is: power 4000W, frequency: 20KHz, working hour 20S, interval time 20S;
After testing, pre-enzymolysis solution is 60 DEG C of viscosity numbers in temperature is 31mPas.
(4) in the obtained pre-enzymolysis solution of step (3), thermostableα-amylase is added, the add-on of thermostableα-amylase is that every gram of starch adds 22LU, 106 DEG C of steam ejection liquefaction 8min, then at 100 DEG C of enzymolysis 70min, obtained DE value is the liquefier of 12.
After testing, liquefier is 60 DEG C of viscosity numbers in temperature is 8mPas.
Embodiment 4
A method for enzymatic liquefaction high-concentration starch milk, comprises the steps:
(1) mass concentration adjusting starch milk that adds water in starch is 45%, adjusts pH 6.0, temperature 60 C, obtained high-concentration starch milk;
(2) in the obtained high-concentration starch milk of step (1), a-amylase and Pullulanase is added, a-amylase add-on is that every gram of starch adds 8U, Pullulanase add-on is that every gram of starch adds 6ASPU, stirs, obtained enzyme-added starch milk;
(3) by the enzyme-added starch milk enzymolysis 40min that step (2) is obtained, obtained pre-enzymolysis solution;
After testing, pre-enzymolysis solution is 60 DEG C of viscosity numbers in temperature is 40mPas.
(4) in the obtained pre-enzymolysis solution of step (3), thermostableα-amylase is added, the add-on of thermostableα-amylase is that every gram of starch adds 22LU, 106 DEG C of steam ejection liquefaction 8min, then at 100 DEG C of enzymolysis 70min, obtained DE value is the liquefier of 12.
After testing, liquefier is 60 DEG C of viscosity numbers in temperature is 18mPas.
Comparative example
A method for enzymatic liquefaction high-concentration starch milk, comprises the steps:
(1) mass concentration adjusting starch milk that adds water in starch is 45%, adjusts pH 6.0, temperature 60 C, obtained high-concentration starch milk;
(2) in the obtained high-concentration starch milk of step (1), add a-amylase, a-amylase add-on is that every gram of starch adds 8U, stirs, obtained enzyme-added starch milk;
(3) by the enzyme-added starch milk enzymolysis 40min that step (2) is obtained, obtained pre-enzymolysis solution;
After testing, pre-enzymolysis solution is 60 DEG C of viscosity numbers in temperature is 45mPas.
(4) in the obtained pre-enzymolysis solution of step (3), thermostableα-amylase is added, the add-on of thermostableα-amylase is that every gram of starch adds 22LU, 106 DEG C of steam ejection liquefaction 8min, then at 100 DEG C of enzymolysis 70min, obtained DE value is the liquefier of 5.
After testing, liquefier is 60 DEG C of viscosity numbers in temperature is 22mPas.
Interpretation of result
As can be seen from the detected result of above-described embodiment 1 ~ 4 and comparative example, add after Pullulanase carries out pre-treatment, significantly can reduce the viscosity number of pre-enzymolysis solution; And can be found out by the detected result of embodiment 3 and embodiment 4, by ultrasonication, enzymolysis efficiency can be promoted, thus significantly reduce the viscosity number of pre-enzymolysis solution.
Claims (10)
1. a method for enzymatic liquefaction high-concentration starch milk, is characterized in that, comprises the steps:
(1) mass concentration adjusting starch milk that adds water in starch is 40 ~ 50%, adjusts pH 5.5 ~ 6.5, temperature 50 ~ 70 DEG C, obtained high-concentration starch milk;
(2) in the obtained high-concentration starch milk of step (1), a-amylase and Pullulanase is added, a-amylase add-on is that every gram of starch adds 4 ~ 10U, Pullulanase add-on is that every gram of starch adds 4 ~ 8ASPU, stirs, obtained enzyme-added starch milk;
(3) by enzyme-added starch milk enzymolysis 30 ~ 50min that step (2) is obtained, obtained pre-enzymolysis solution;
(4) in the obtained pre-enzymolysis solution of step (3), thermostableα-amylase is added, the add-on of thermostableα-amylase is that every gram of starch adds 18 ~ 25LU, 105 DEG C ~ 107 DEG C steam ejection liquefaction 5 ~ 10min, then at 95 DEG C ~ 102 DEG C enzymolysis 45 ~ 90min, obtained DE value is the liquefier of 10 ~ 15.
2. the method for claim 1, is characterized in that, in described step (1), the mass concentration of starch milk is 43 ~ 47%.
3. the method for claim 1, is characterized in that, in described step (2), a-amylase is middle temperature a-amylase.
4. the method for claim 1, it is characterized in that, in described step (2), Pullulanase is the Pullulanase that Wuxi biotechnology company limited of Jie Neng section produces, and a-amylase is the middle temperature a-amylase that Wuxi biotechnology company limited of Jie Neng section produces.
5. the method for claim 1, is characterized in that, in described step (2), adds a-amylase and Pullulanase carries out under agitation.
6. the method for claim 1, is characterized in that, in described step (2), a-amylase add-on is that every gram of starch adds 6 ~ 8U, and Pullulanase add-on is that every gram of starch adds 5 ~ 7ASPU.
7. the method for claim 1, is characterized in that, also comprises the step of carrying out ultrasonication in described step (3) enzymolysis process.
8. method as claimed in claim 7, it is characterized in that, described ultrasonication condition is: power 3000 ~ 5000W, frequency: 20KHz, working hour 10 ~ 30S, interval time 10 ~ 20S, ultrasonic treatment time is identical with the enzymolysis time of step (3);
Preferably, described ultrasonication condition is: power 5000W, frequency: 20KHz, working hour 30S, interval time 10S.
9. method as claimed in claim 7, it is characterized in that, described ultrasonication is for carry out under agitation.
10. the method for claim 1, is characterized in that, in described step (4), thermostableα-amylase is the efficient high-temperature resistant a ?amylase that Wuxi biotechnology company limited of Jie Neng section produces.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410583587.3A CN104328156A (en) | 2014-10-27 | 2014-10-27 | Method of liquefying high-concentration starch milk by enzymatic process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410583587.3A CN104328156A (en) | 2014-10-27 | 2014-10-27 | Method of liquefying high-concentration starch milk by enzymatic process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104328156A true CN104328156A (en) | 2015-02-04 |
Family
ID=52402960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410583587.3A Pending CN104328156A (en) | 2014-10-27 | 2014-10-27 | Method of liquefying high-concentration starch milk by enzymatic process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104328156A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104911234A (en) * | 2015-06-02 | 2015-09-16 | 吉林大学 | Method for efficiently liquefying high-concentration corn starch step by step |
| CN106119316A (en) * | 2016-06-23 | 2016-11-16 | 江南大学 | A kind of high concentration starch saccharification that improves produces the method for maltose yield in maltose syrup |
| CN106832435A (en) * | 2017-01-22 | 2017-06-13 | 福建农林大学 | A kind of processing method of lotus seed starch lipid complex nano particle |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1911966A (en) * | 2006-08-23 | 2007-02-14 | 华南理工大学 | Pretreatment method of starch |
| CN101133785A (en) * | 2007-09-26 | 2008-03-05 | 烟台大学 | Method for producing concentrated barley juice |
| CN103842516A (en) * | 2011-09-29 | 2014-06-04 | 丹尼斯科美国公司 | Liquefaction and saccharification of granular starch at high concentration |
-
2014
- 2014-10-27 CN CN201410583587.3A patent/CN104328156A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1911966A (en) * | 2006-08-23 | 2007-02-14 | 华南理工大学 | Pretreatment method of starch |
| CN101133785A (en) * | 2007-09-26 | 2008-03-05 | 烟台大学 | Method for producing concentrated barley juice |
| CN103842516A (en) * | 2011-09-29 | 2014-06-04 | 丹尼斯科美国公司 | Liquefaction and saccharification of granular starch at high concentration |
Non-Patent Citations (3)
| Title |
|---|
| 刘文静: "微波预处理对玉米淀粉液化的影响", 《食品与发酵工业》 * |
| 张文博: "超声辅助处理高温淀粉酶水解玉米淀粉制备麦芽糊精的工艺研究", 《粮油加工》 * |
| 张礼星: "一种经济实用的制备高浓度糖液的方法", 《江苏食品与发酵》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104911234A (en) * | 2015-06-02 | 2015-09-16 | 吉林大学 | Method for efficiently liquefying high-concentration corn starch step by step |
| CN106119316A (en) * | 2016-06-23 | 2016-11-16 | 江南大学 | A kind of high concentration starch saccharification that improves produces the method for maltose yield in maltose syrup |
| CN106832435A (en) * | 2017-01-22 | 2017-06-13 | 福建农林大学 | A kind of processing method of lotus seed starch lipid complex nano particle |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Swathy et al. | Production and optimization of high grade cellulase from waste date seeds by Cellulomonas uda NCIM 2353 for biohydrogen production | |
| de França Passos et al. | A comparative review of recent advances in cellulases production by Aspergillus, Penicillium and Trichoderma strains and their use for lignocellulose deconstruction | |
| CN106148425B (en) | The preparation method of inositol | |
| Xu et al. | An ionic liquid tolerant cellulase derived from chemically polluted microhabitats and its application in in situ saccharification of rice straw | |
| CN101495642B (en) | Native grain amylases in enzyme combinations for granular starch hydrolysis | |
| Grande et al. | Enzymatic hydrolysis of microcrystalline cellulose in concentrated seawater | |
| Outeirino et al. | A novel approach to the biorefinery of brewery spent grain | |
| EP2702161A1 (en) | Use of cellulase and glucoamylase to improve ethanol yields from fermentation | |
| CN103952452B (en) | A kind of preparation method of environment-friendly type high-purity oligoisomaltose | |
| CN1840674A (en) | Process for preparing xylo-oligosaccharide by enzymolysis of wheat bran | |
| Zhang et al. | Assessment of enzyme addition strategies on the enhancement of lipid yield from microalgae | |
| CN105218248A (en) | A kind of preparation method of biological and ecological methods to prevent plant disease, pests, and erosion active liquid seaweed fertilizer | |
| Li et al. | The hydrolytic efficiency and synergistic action of recombinant xylan-degrading enzymes on xylan isolated from sugarcane bagasse | |
| CN104328156A (en) | Method of liquefying high-concentration starch milk by enzymatic process | |
| CN101939442A (en) | Ph adjustment free system for producing fermentable sugars and alcohol | |
| CN102321704A (en) | Method for treating starchy raw material and method for preparing citric acid | |
| CN105542033A (en) | Preparation method of low-methoxyl citrus pectin | |
| Yan et al. | A novel thermostable β-1, 3-1, 4-glucanase from Thermoascus aurantiacus and its application in oligosaccharide production from oat bran | |
| El-Ghonemy | Optimization of extracellular ethanol-tolerant β-glucosidase production from a newly isolated Aspergillus sp. DHE7 via solid state fermentation using jojoba meal as substrate: purification and biochemical characterization for biofuel preparation | |
| CN104630189B (en) | A kind of carbohydrase Pullulanase joint product and its production method | |
| TWI530562B (en) | Preparation method conducive to enhancing enzymatic activity of cellulase | |
| Aytaş et al. | Partial characterization of β-glucosidase, β-xylosidase, and α-l-arabinofuranosidase from Jiangella alba DSM 45237 and their potential in lignocellulose-based biorefining | |
| CN112920285B (en) | Preparation method and application of rice bran polysaccharide | |
| WO2018133619A1 (en) | Method for producing sugar by hydrolyzing thick mash of agricultural and forest biomass raw material | |
| Cai et al. | Using highly recyclable sodium caseinate to enhance lignocellulosic hydrolysis and cellulase recovery |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20160318 Address after: Dickson street 251200 Shandong city of Dezhou province Yucheng city high tech Development Zone Applicant after: Shandong Bailong Chuangyuan Biotechnology Co.,Ltd. Address before: 251200 Fuhua street, hi tech Zone, Dezhou, Shandong, Yucheng Applicant before: SHANDONG GUANGBO BIOTECHNOLOGY SERVICE CO., LTD. |
|
| CB02 | Change of applicant information |
Address after: 251200 Shandong province Dezhou Dexin Street (Yucheng) National hi tech Industrial Development Zone Applicant after: Shandong Bailong Park biological Polytron Technologies Inc Address before: Dickson street 251200 Shandong city of Dezhou province Yucheng city high tech Development Zone Applicant before: Shandong Bailong Chuangyuan Biotechnology Co.,Ltd. |
|
| COR | Change of bibliographic data | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150204 |
|
| RJ01 | Rejection of invention patent application after publication |