CN104911191A - Fertility regulation gene FG3 and application thereof - Google Patents

Abstract

The invention relates to a fertility gene and an application thereof, which belong to the technical field of biology, and more specifically relates to a method for crossbreeding plants, the method comprises sterile line propagation and hybrid seed preparation, and the invention more specifically relates to a fertility regulation gene FG3, and its mutant and an application thereof in crossbreeding.

Description

A kind of sterility changing gene FG3 and application thereof

Technical field

The invention belongs to biological technical field, be specifically related to plant hybridization breeding method, comprise sterile line propagation and cenospecies preparation, relate more specifically to a fertile gene FG3 and mutant thereof and the application in cross-breeding thereof.

Technical background

Cross-breeding is the effective way improving production estimation, and cross-fertilize seed has obvious output, resistance and adaptability advantage than routine kind usually, usual also, the instant effect shorter than the routine kind seed selection cycle of seed selection of cross-fertilize seed.Current cross-breeding has become the main breeding method of many crops.

The seed selection of crop male sterile line is the key link of cross-breeding.Male sterile line is male gametophyte development defect, the normotrophic plant individual of megagamete, accepts the pollen from male parent as female parent.The seed selection of male sterile line needs to consider several factor: 1, hybridize combo: sterile line and corresponding paternal plant combine the filial generation producing and have good character; 2, the breeding of sterile line: sterile line can recover fertility under certain condition makes it be maintained; 3, sterile line self-reproduction and hybrid seeding efficiency: good sterile line must be easy to breeding, and hybrid seed production yields is high.Male sterile line at present for paddy rice cross breeding breeding comprises cytoplasmic male sterile line and nucleus sterile line.Cytoplasmic male sterility cross-breeding system relates to male sterile line, restorer and maintenance line, i.e. so-called three series.Triple crossing needs specific restorer and maintenance line, and not only production of hybrid seeds process is complicated, also greatly limit the utilization of hybrid vigour to different varieties resource.The sterile line that the TwoLine Method corresponding with three series utilizes nuclear gene to control and this sterile line recover the feature of fertility under particular growth envrionment conditions, and restorer and maintenance line are united two into one.Compared with " three series ", " bilinear method " has obvious advantage, both eliminates maintenance line and simplifies the production sequence of cenospecies, and greatly extending the range of choice of male parent, various good character can be combined in filial generation.

Key characteristic for the sterile line of double-line hybrid is: sterile line keeps sterility under certain conditions, can be used for hybrid seeding; And sterile line can recover fertility self-reproduction during condition change, reach the object keeping this sterile line.The sterile line adopted in current double-line hybrid mostly is photo-thermo-sensitive genetic male sterile line, its fertility is by temperature and light according to variable effect, and the instability of these environmental factorss can affect the stability of sterile line, causes self-fertility, reduce the purity of cenospecies, thus risk in hybrid seed production is increased.And the sterile line for double-line hybrid utilizing current technology to select is very limited, does not almost have good double-line hybrid to combine in such as japonica rice variety, limit making full use of of variety source.

In order to solve the defect existed in current hybrid rice seed breeding method, as stability, the limitation of Hybrid resource, seeding technique complexity, the breeding cost high-technology bottleneck of sterile line, people are attempting new crossbreeding technology, novel crossbreeding technology will make full use of the male sterility gene of recessive nuclear gene control, build the sterile line that stable fertility is not affected by environment, remove environmental factors to the restriction of cross-breeding, eliminate the potential risk on producing; Meanwhile, the Recessive Male sterility utilized should be applicable to most kind, the hybrid vigour utilization of resources is significantly improved, solves heterotic resource utilization issue; The male sterile line that namely the present invention provides a kind of crop fertile gene and produce based on this transgenation, the stable fertility of this sterile line, not by environmental influence, can be recovered by wild-type transgenic.The sterile element being the novel cross-breeding system of structure and providing necessity that this gene and this transgenation produce.

Invention summary

The invention provides a kind of DNA sequence dna, described DNA sequence dna has the function of regulating plant fertility, it is characterized in that, described DNA sequence dna is selected from one of sequence of following group:

A) there is the nucleotide sequence shown in SEQ ID NO:1 or 5;

B) there is the nucleotide sequence shown in SEQ ID NO:10 or 11;

C) there is the nucleotide sequence shown in SEQ ID NO:13 or 14;

D) there is the nucleotide sequence shown in SEQ ID NO:16 or 17;

E) there is the nucleotide sequence shown in SEQ ID NO:19;

F) there is the nucleotide sequence shown in SEQ ID NO:21 or 22;

G) under strict conditions can with the DNA sequence dna of the DNA hybridization of the arbitrary described sequence of (a)-(f); Or

H) with the DNA sequence dna of the arbitrary described complementary of (a)-(g).

The aminoacid sequence of above-mentioned DNA sequence encoding is as shown in SEQ ID NO:2,6,12,15,18,20 or 23.

Present invention also offers a kind of expression cassette, it is characterized in that described expression cassette comprises above-mentioned DNA sequence dna.

Present invention also offers a kind of expression vector, it is characterized in that described expression vector comprises above-mentioned expression cassette.

Present invention also offers a kind of engineering bacteria, it is characterized in that described engineering bacteria comprises above-mentioned expression vector.

Present invention also offers the application of a kind of gene in plant fertility regulation and control, it is characterized in that, the nucleotide sequence of described sterility changing gene is selected from one of sequence of following group:

A) there is the nucleotide sequence shown in SEQ ID NO:1 or 5;

B) there is the nucleotide sequence shown in SEQ ID NO:10 or 11;

C) there is the nucleotide sequence shown in SEQ ID NO:13 or 14;

D) there is the nucleotide sequence shown in SEQ ID NO:16 or 17;

E) there is the nucleotide sequence shown in SEQ ID NO:19;

F) there is the nucleotide sequence shown in SEQ ID NO:21 or 22;

G) under strict conditions can with the DNA sequence dna of the DNA hybridization of the arbitrary described sequence of (a)-(f); Or

H) with the DNA sequence dna of the arbitrary described complementary of (a)-(g).

The present invention also comprises a kind of method being obtained male sterile material by sudden change sterility changing genes of SEQ ID NO:1,5,10,11,13,14,16,17,19,21 or 22.

The nucleotide sequence that " sudden change " described in the present invention is included in sterility changing gene carries out replacing, lack or add one or more Nucleotide.

Present invention also offers the male sterile that a kind of recovery is caused by transgenation shown in corresponding SEQ ID NO:1,5,10,11,13,14,16,17,19,21 or 22, make malesterile mutants revert to the method that can educate.

The present invention also comprises a kind of application of mutant material, it is characterized in that described mutant material caused by the sudden change of nucleotide sequence, and described nucleotide sequence is as shown in SEQ ID NO:1,5,10,11,13,14,16,17,19,21 or 22.

Above-mentioned " sudden change " can be point mutation, and also can be DNA disappearance or insertion mutation, also can be produced by the gene silencing such as RNAi, rite-directed mutagenesis means.

Present invention also offers method above-mentioned materials and DNA sequence dna are applied in breeding, more specifically described application refers to mutant plants maternal as sterile line, hybridize with restorer, production cenospecies.

Present invention also offers a kind of promotor, described promotor has the characteristic of flower pesticide specifically expressing, and its nucleotide sequence is as shown in SEQ ID NO:3 or 9, and the present invention also comprises the expression cassette, carrier and/or the engineering strain that comprise above-mentioned promotor.

Present invention also offers a kind of method expressing object nucleotide sequence in plant, described method comprises to plant materials importing DNA construct, described DNA construct contains the object nucleotide sequence that promotor and operability are connected to described promotor, and the nucleotide sequence of wherein said promotor is as shown in SEQ ID NO:3 or 9.

Described " object nucleotide sequence " can be structure gene, regulatory gene, the inverted defined gene of structure gene, the inverted defined gene of regulatory gene or the tiny RNA that native gene can be disturbed to express, it is at specific expressed fertility and the pollen germination that can regulate pollen in pollen development late period.

The present invention also comprise above-mentioned DNA sequence dna and or the application of promotor in any one of following (a) to (d):

A () cultivates plants kind or strain;

B () cultivates plant variety or the strain of the enhancing of Pollination Fertilization ability;

C plant variety that () cultivation Pollination Fertilization ability slackens or strain;

D () cultivates male sterile plants kind or strain.

Present invention also offers a kind of for keeping the method for the homozygous recessive condition of male sterile plants (method detailed can consult PCT patent PCT/CN2013/086657), described method comprises:

A) provide the first plant, it comprises the homozygous recessive alleles of FG3 gene, and it is male sterile;

B) in the first plant, introduce following construct, form the second plant, described second plant comprises the homozygous recessive alleles of FG3 gene and described construct, and construct is hemizygous condition in the second plant, and described construct comprises:

I) the first nucleotide sequence, it comprises FG3 nucleotide sequence, and when expressing in the first plant, it will recover male fertility;

Ii) the second nucleotide sequence, when it is expressed, can suppress formation or the function that can educate male gamete in described second plant, be specially pollen inactivated gene ZM-PA; And

C) described first plant fertilization is made, to produce the offspring maintaining described first plant homozygous recessive condition with the male gamete of described second plant.

Accompanying drawing explanation

Fig. 1 Osfg3 mutant and the yellow Hua Zhan little floral shape of wild-type.

Fig. 2 Osfg3 mutant and the yellow China of wild-type account for flower pesticide form.

Fig. 3 Osfg3 mutant and the yellow China of wild-type account for pollen staining analysis.

Fig. 4 Osfg3 mutant and the yellow China of wild-type account for female organ morphology and compare.

Fig. 5 Osfg3 mutant stigma appearing situation, arrow refers to the column cap exposed.

Fig. 6 is that the yellow China of Osfg3 mutant and wild-type accounts for blade trichome and compares, and arrow refers to blade trichome.

Fig. 7 is that the yellow China of Osfg3 mutant and wild-type accounts for glumelle trichome and compares, and arrow refers to glumelle trichome.

Fig. 8 is the cDNA sequence comparison result that yellow China accounts for OsFG3, Osfg3 mutant and the fine Osfg3 of Japan, and wherein HHZ represents that yellow China accounts for OsFG3 sequence, and Mutant represents Osfg3 mutant sequence, Nip represent Japan fine in Osfg3 sequence.

Fig. 9 is the sequence alignment of protein result that yellow China accounts for OsFG3, Osfg3 mutant and the fine Osfg3 of Japan, and wherein HHZ represents that yellow China accounts for Osfg3 sequence, and Mutant represents Osfg3 mutant sequence, Nip represent Japan fine in OsFG3 sequence.

The expression level analysis of Figure 10 OsFG3 in different rice tissue organ.

The differential expression analysis of Figure 11 OsFG3 gene in mutant and wild-type.

The promoter expression vector of Figure 12 OsFG3 gene.

The transgenic function complementation carrier of Figure 13 rice male sterility mutant (Osfg3).

The RNAi interference carrier of Figure 14 OsFG3 gene.

The albumen of Figure 15 OsFG3 genes encoding and barley (Hordeum vulgare), Chinese sorghum (Sorghum bicolor), millet (millet) (Setaria italica), two fringe false bromegrasses (Brachypodium distachyon), the sequence alignment figure of the homologous protein in corn (Zea mays) genome, 1 is paddy rice (Oryza sativa), 2 is barley (Hordeum vulgare), 3 is Chinese sorghum (Sorghum bicolor), 4 is millet (millet) (Setaria italica), 5 is two fringe false bromegrasses (Brachypodium distachyon), 6 is corn (Zea mays).

Detailed Description Of The Invention

All reference mentioned in this article are incorporated to herein all by reference.

Unless there are indicating on the contrary, all technology used herein and scientific terminology all have understood identical implication usual with one skilled in the art of the present invention.Unless there are indicating on the contrary, technology that is used herein or that mention is standard technique known to a person of ordinary skill in the art.Material, method and example are only used as to set forth, but not are limited.

The present invention includes a kind of fertility-related gene and Nucleotide thereof and protein sequence, also comprising by operating the application of this gene in regulation and control plant male fertility.Without limitation for example, any method described below all can use together with corresponding nucleotide sequence provided by the present invention, such as, the mutant sequence of described fertile gene is introduced plant causing plant male sterile, make plant endogenous sequence suddenly change, introduce in plant this sequence antisense sequences, use hair fastener form or couple together its and other nucleotide sequence to regulate and control the phenotype of plant or the either method that can be used in the multiple method of the male fertility affecting plant well known by persons skilled in the art.

Fertile gene FG3 provided by the present invention is the gene that a pollen development is relevant.In paddy rice, this fertile gene is positioned on No. 10 karyomit(e), and its nucleotide sequence in long-grained nonglutinous rice is as shown in SEQ ID NO:1 or 4, and aminoacid sequence is as shown in SEQ ID NO:2; In japonica rice, its nucleotide sequence is as shown in SEQ ID NO:5, and its aminoacid sequence is as shown in SEQ ID NO:6; In barley, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:10 or 11, and its aminoacid sequence is as shown in SEQ ID NO:12; In Chinese sorghum, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:13 or 14, and its aminoacid sequence is as shown in SEQ ID NO:15; In corn, the nucleotide sequence of this fertile gene ZmFG3 is as shown in SEQ ID NO:16 or 17, and its aminoacid sequence is as shown in SEQ ID NO:18; In millet, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:19, and its aminoacid sequence is as shown in SEQ ID NO:20; In two fringe false bromegrasses, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:21 or 22, and its aminoacid sequence is as shown in SEQ ID NO:23.

The present invention also comprises one of sequence of following group: a) have at least 90% (being preferably at least 95%) sequence similarity with above-mentioned FG3 gene order, and have the DNA sequence dna of identical function; B) under strict conditions can with the DNA sequence dna of the DNA hybridization of sequence (a) described; C) with the DNA sequence dna of above-mentioned arbitrary described complementary.

Fertile gene described above, can be separated and obtain from each kind of plant.Those skilled in the art should know, and fertility restorer gene of the present invention comprises and FG3 gene very high homology, and have the function equivalence body sequence of the very high homology of same sterility changing function.The function equivalence body sequence of described very high homology to be included under high stringency conditions can with the DNA sequence dna of the nucleotide sequence hybridization of FG3 gene disclosed in this invention." high stringency conditions " that use in the present invention is known, comprise and such as in the hybridization solution containing 400mM NaCl, 40mM PIPES (pH6.4) and 1mM EDTA, hybridize 12-16 hour in 60 DEG C, then at 65 DEG C, wash 15-60 minute with the washings containing 0.1SDS and 0.1%SSC.

Function equivalence body sequence also comprises has at least 90%, 95%, 96%, 97%, 98% or 99% sequence similarity with the sequence shown in FG3 gene disclosed in this invention, and there is the DNA sequence dna of sterility changing function, can be separated from any plant and obtain.Wherein, the per-cent of sequence similarity can be obtained by known bioinformatics, comprise Myers and Miller algorithm (Bioinformatics, 4 (1): 11-17, 1988), Needleman-Wunsch overall comparison method (J.Mol.Biol., 48 (3): 443-53, 1970), Smith-Waterman Local Alignment method (J.Mol.Biol., 147:195-197, 1981), Pearson and Lipman similarity-searching (PNAS, 85 (8): 2444-2448, 1988), algorithm (the Altschul etc. of Karlin and Altschul, J.Mol.Biol., 215 (3): 403-410, 1990, PNAS, 90:5873-5877,1993).This is familiar to those skilled in the art.

Gene order of the present invention can be separated and obtain from any plant, include but not limited to Btassica, corn, wheat, Chinese sorghum, two joint shepherd's purses belong to, sinapsis alba, Semen Ricini, sesame, cottonseed, Semen Lini, soybean, Arabidopsis, Phaseolus, peanut, clover, oat, Semen Brassicae campestris, barley, oat, rye (Rye), grain, chinese sorghum, triticale, einkorn, Si Peierte wheat (Spelt), emmer, flax, gramagrass (Gramma grass), friction standing grain, false chinese sorghum, fescue grass, perennial ryegrass, sugarcane, crowberry, papaya, banana, safflower, oil palm, muskmelon, apple, cucumber, the stem of noble dendrobium, gladiolus, chrysanthemum, Liliaceae, cotton, eucalyptus, Sunflower Receptacle, rape, beet, coffee, ornamental plant and conifer etc.Preferably, plant comprises corn, soybean, safflower, leaf mustard, wheat, barley, rye, rice, cotton and Chinese sorghum.

Present invention also offers nucleotide sequence by affecting FG3 or affect the method for plant fertility by the transcriptional expression of regulation and control FG3 gene.The described plant fertility that affects refers to expression by regulation and control FG3 gene, thus the fertility of described plant is changed, as caused plant male sterile.Particularly, depend on embody rule demand, the expression of FG3 gene in plant materials can be affected by multiple method, thus reach the effect of regulation and control plant male fertile.More specifically, the expression of regulation and control FG3 gene can use the obtainable instrument of many those of ordinary skill in the art to carry out, such as, by the introducing etc. of sudden change, mutagenesis, the proceeding to of inverted defined gene, co-suppression or hairpin structure, may be used to the normal expression destroying FG3 gene, thus obtain male sterile plant.On the other hand, the present invention also comprises by the nucleotide sequence of wild-type FG3 being introduced the male fertility that plant recovers the destroyed plant of FG3 expression.

Present invention also offers a kind of sterile mutant sequence and malesterile mutants material thereof of FG3 gene.More specifically, described malesterile mutants material is by the endogenous FG3 gene of sudden change paddy rice, or the nucleotide sequence of the gene of sudden change and its very high homology, makes this plant materials lose the process of male fertile.Described " sudden change " includes but not limited to following methods, as the transgenation caused by the method for physics or chemistry, chemical process comprises the mutagenesis caused with mutagenic compound process such as EMS, described sudden change can also be point mutation, also can be DNA disappearance or insertion mutation, can also be produced by the gene silencing such as RNAi, rite-directed mutagenesis means.

Particularly, present invention also offers a kind of rice male sterility mutant, it contains the male sterility gene after sudden change, and the nucleotide sequence of the male sterility gene after described sudden change is as shown in SEQ ID NO:7, and aminoacid sequence is as shown in SEQ ID NO:8.Compared with wild-type, in sterile mutant, the shearing site of this gene the 8th and the 9th exon is by GT ... AG sports AT ... AG (Fig. 8), makes corresponding intron can not be sheared (Fig. 8), causes the premature termination of this gene.。Those skilled in the art should know, and described nucleotide sequence SEQ ID NO:7 can be building up to plant expression vector, carry out Plant Transformation, thus obtain new genetically modified malesterile mutants material.

Present invention also offers a kind of promotor of FG3 gene, described promotor has the function of specifically expressing in pollen, and the sequence of corresponding promotor is that FG3 gene is from ATG to the nucleotide sequence of upstream about 700bp to 2500bp.More specifically, in paddy rice, the nucleotide sequence of described OsFG3 gene promoter is as shown in SEQ ID NO:3 or SEQ ID NO:9.SEQ ID NO:3 or SEQ ID NO:9 is connected with Reporter gene GUS, conversion of plant, detect the GUS expression activity in analysis transfer-gen plant and expression pattern, by transfer-gen plant root, stem, leaf and all carry out GUS staining analysis in spending, found that OsFG3 promoters driven gus gene is mainly expressed in Rice Anther, more specifically at the P9 phase specific high expression level of anther development.Illustrate that SEQ ID NO:3 provided by the present invention or SEQ ID NO:9 promotor are the promotors of an anther-specific expression.

Plant pollen specific expression promoter provided by the present invention, containing the nucleotide sequence shown in SEQ ID NO:3 in ordered list or SEQ ID NO:9, or comprise the nucleotide sequence with listed nucleotide sequence in SEQ ID NO:3 or SEQ ID NO:9 with more than 90% similarity, or comprise 100 and more than 100 continuous print nucleotide fragments deriving from SEQ ID NO:3 or SEQ ID NO:9 sequence, and the nucleotides sequence be connected with this promotor operability can be driven to be listed in expression in plant pollen.Expression vector containing above-mentioned sequence, transgenic cell line and Host Strains etc. all belong to protection scope of the present invention.The primer pair of arbitrary nucleotide fragments of SEQ ID NO:3 disclosed in this invention or the SEQ ID NO:9 promotor of increasing is also within protection scope of the present invention.

Promotor nucleotide sequence provided by the present invention also can be used for being separated corresponding sequence from other plant beyond paddy rice, especially from other monocotyledonss, carries out homologous clone.According to these corresponding sequence and the sequence homology herein between listed promoter sequence, or with the homology of this promoter gene, use such as the technology such as PCR, hybridization is differentiated to be separated these corresponding sequence.Therefore, according to the respective segments that they are separated with the SEQ ID NO:3 listed by the present invention or the sequence similarity between SEQ ID NO:9 promoter sequence (or its fragment), be also included within embodiment.

" promotor " of the present invention refers to that a kind of DNA regulates and controls region, and it comprises the TATA box of energy guide RNA polymerase II in the initial RNA synthesis of the suitable transcription initiation site of specific coding sequence usually.Promotor also can comprise other recognition sequence, and these recognition sequences are usually located at upstream or the 5 ' end of TATA box, are commonly called upstream promoter element, play regulatory transcription efficiency.Those skilled in the art should know, although identified the nucleotide sequence for promoter region disclosed by the invention, separation andpreconcentration has been in other controlling element of the TATA box upstream region of the specific promoter region of the present invention's qualification also within the scope of the invention.Therefore, promoter region disclosed herein is defined as usually further comprises upstream regulatory elements, such as, for those element, enhansers etc. of the tissue expression and temporal expressions function that regulate and control encoding sequence.In an identical manner, can identify, isolate the promoter element making to carry out expressing in destination organization (such as male tissue), it be used together with other core promoter, to verify the expression that male tissue is preferential.Core promoter refers to the minimal sequence needed for initiation transcription, such as, be called as the sequence of TATA box, and this is that the promotor of the gene of coded protein all has usually.Therefore, alternatively, FG3 gene upstream promoter can with himself or associate from other core promoter of originating and use.

Core promoter can be the known core promoter of any one, such as cauliflower mosaic virus 35S or 19S promotor (U.S. Patent No. 5,352,605), ubiquitin promoter (U.S. Patent No. 5,510,474), IN2 core promoter (U.S. Patent No. 5,364,780) or figwort mosaic virus promotor.

The function of described gene promoter can be analyzed by the following method: be connected with reporter gene operability by promoter sequence, form transformable construct, again this construct is proceeded in plant, in acquisition transgenic progeny, confirm its expression characterization by the expression of visual report gene in each histoorgan of plant; Or above-mentioned construct subclone is entered the expression vector for transient expression experiment, is detected the function of promotor or its control region by transient expression experiment

Being used for the selection of suitable expression vector of test starting or control region domain-functionalities will depend on host and this expression vector will be introduced the method for host, and these class methods are well known to those of ordinary skill in the art.For eukaryote, region in the carrier comprises the region controlling transcription initiation and controlled working.These regions are operably connected to reporter gene, and described reporter gene comprises YFP, UidA, gus gene or luciferase.The expression vector comprising the presumption control region being arranged in genomic fragment can be introduced into complete tissue, such as interim pollen, or introduces callus, to carry out functional verification.

In addition, promotor of the present invention can be connected with the nucleotide sequence being not FG3 gene, to express other heterologous nucleotide sequence.Promotor nucleotide sequence of the present invention and fragment thereof can be assembled in an expression cassette with variant together with heterologous nucleotide sequence, for expressing in object plant, more specifically, express in the male organs of this plant.Described expression cassette has suitable restriction enzyme site, for inserting described promotor and heterologous nucleotide sequence.These expression cassettes can be used for carrying out genetic manipulation to any plant, to obtain the corresponding phenotype wanted.

FG3 promotor disclosed in this invention is more specifically the FG3 promotor in paddy rice, can be used for the expression driving following heterologous nucleotide sequence, obtains male sterile phenotype to make the plant of conversion.Described heterologous nucleotide sequence codified impels enzyme or modifying enzyme, amylase, debranching factor and the polygalacturonase of carbohydrate degradation, more specifically as a amylase gene, growth hormone (auxin), rot B, cytotoxin gene, diphtheria toxin, DAM methylase, avidin, or can be selected from protokaryon regulator control system, can also be dominant male sterility gene.

In some embodiments, the nucleic acid being operatively coupled on promotor downstream of the present invention mentioned in the present invention, wherein said " nucleic acid " can be the tiny RNA that operability is connected to structure gene on promotor disclosed herein, regulatory gene, the inverted defined gene of structure gene, the inverted defined gene of regulatory gene or native gene can be disturbed to express.

More specifically, sterility changing genes of SEQ ID NO:1 provided by the present invention and SEQ ID NO:5 can be building up to the downstream of promotor SEQ ID NO:3 or SEQ ID NO:9, thus drive the specifically expressing of this sterility changing gene in pollen, or by the know-why of RNAi, build by SEQ ID NO:3 or SEQ ID NO:9 start can the RNAi carrier of reticent SEQ ID NO:1 gene, thus obtain the malesterile mutants of SEQ ID NO:1 gene.

Promoter sequence provided by the present invention is separable from any plant, include but not limited to Btassica, corn, wheat, Chinese sorghum, two joint shepherd's purses belong to, sinapsis alba, Semen Ricini, sesame, cottonseed, Semen Lini, soybean, Arabidopsis, Phaseolus, peanut, clover, oat, Semen Brassicae campestris, barley, oat, rye (Rye), grain, chinese sorghum, triticale, einkorn, Si Peierte wheat (Spelt), emmer, flax, gramagrass (Gramma grass), friction standing grain, false chinese sorghum, fescue grass, perennial ryegrass, sugarcane, crowberry, papaya, banana, safflower, oil palm, muskmelon, apple, cucumber, the stem of noble dendrobium, gladiolus, chrysanthemum, Liliaceae, cotton, eucalyptus, Sunflower Receptacle, rape, beet, coffee, ornamental plant and conifer etc.Preferably, plant comprises corn, soybean, safflower, leaf mustard, wheat, barley, rye, rice, cotton and Chinese sorghum.

The present invention also comprises the construct containing FG3 gene and/or its promotor, and described construct comprises usually said carrier or expression cassette.The promotor that promotor in described construct can be natural promoter or be substituted, its by drive connect nucleotides sequence and be listed in expression in plant.Promotor in construct can be inducible promoter.When being connected with another promotor by the nucleotide sequence of FG3 gene, preferably, this promotor fully drives the expression of this sequence in early days at pollen development, such as can be specific expressed in the P9 phase of pollen development.Particularly, the kind of spendable promotor comprises composing type viral promotors, such as cauliflower mosaic virus (CaMV) 19S and 35S promoter, or radix scrophulariae mosaic virus 35 S promoter, or ubiquitin promoter.

Organizing specific expression promotor can be used for strengthening in the specific plant tissue of target transcribing and/or expressing.Promotor can be expressed in targeted tissue and also express in other plant tissue, can strong expression and the expression of organizing degree much lower than other in targeted tissue, or can highly preferably express in targeted tissue.In one embodiment, promotor is the type of preference specifically expressing in the male of plant or female tissue.The present invention must not use any specific male tissue type of priority promotor in method, and any in this type of promotor a lot of well known by persons skilled in the art can use.Natural FG3 promotor described herein is an example of spendable promotor.This type of promotor another kind of is 5126 promotors, MS45 promotor, MS26 promotor, BS92-7 promotor, SGB6 controlling element and TA29 promotor etc., and it prefers to the expression of gene in male plant tissue instructing it to connect.Gamete can also be comprised in some construct organize and preferentially express promotor.Male gamete is preferentially expressed promotor and is comprised PG47 promotor and ZM13 promotor.

Also can comprise other component in above-mentioned construct, this depends primarily on object and the purposes of vector construction, such as, can comprise selectable marker gene, target or regulating and controlling sequence, critical sequences or homing sequence, intron etc. further.3 ' the end at desired heterologous nucleotide sequence is also included in plant by expression cassette has transcribing and translation termination of function.Terminator can be the terminator of gene provided by the present invention, also can be the terminator from external source.More specifically, above-mentioned terminator can be nopaline synthase or octopine synthase termination area.

Guide the expression product of heterologous nucleotide sequence into specific cells device in hope, such as plastid, amyloplast, or guide endoplasmic reticulum into, or when cell surface or cell exocrine, expression cassette also can comprise the nucleotide sequence for encoding transit peptides.This type of transit peptides is known in the field, and it includes but not limited to the small subunit, plant EPSP synthase, corn Brittle-1 chloroplast transit peptides etc. of Rubisco.

In the process preparing expression cassette, can be operated multiple DNA fragmentation, be in proper orientation to provide, or be in the DNA sequence dna in correct reading frame.For reaching this object, adapter or joint can be used, DNA fragmentation is linked up, or comprise other operation further, with the restriction enzyme site etc. of providing convenience.

Further, in construct provided by the present invention, also selectable marker gene can be comprised, for selecting the cell or tissue through transforming.Described selectable marker gene comprises gives antibiotics resistance or the gene to Herbicid resistant.Suitable selectable marker gene includes but not limited to: chloramphenicol resistance gene, hygromycin gene, streptomycin resistance gene, miramycin resistant gene, sulfamido resistant gene, glyphosate gene, the bony resistant gene of careless fourth.Described selectable marker gene can also be the genes such as red fluorescent gene, cyan fluorescent protein gene, yellow fluorescent protein gene, luciferase gene, green fluorescence protein gene, anthocyanin p1.

Expression cassette provided by the present invention or carrier can be inserted into plasmid, clay, yeast artificial chromosome, bacterial artificial chromosome or other be applicable to being transformed in any carrier in host cell.Preferred host cell is bacterial cell, such as, in particular for cloning or storing polynucleotide or the bacterial cell for transformed plant cells, intestinal bacteria, Agrobacterium tumefaciems and Agrobacterium rhizogenes.When host cell is vegetable cell, expression cassette or carrier can be inserted in the genome of the vegetable cell be converted.Insertion can be location or random insertion.Preferably, be inserted through such as homologous recombination to realize.In addition, expression cassette or carrier can remain on outside karyomit(e).Expression cassette of the present invention or carrier can be present in the core of vegetable cell, chloroplast(id), plastosome and/or plastid.Preferably, expression cassette of the present invention or carrier are inserted in the chromosomal DNA of plant nucleolus.

The present invention also comprises disclosed FG3 gene and the application of promotor thereof, in the embodiment of some application, FG3 gene provided by the present invention or its promotor can be applied and to suddenly change the breeding of the male sterile line obtained and maintenance to realize FG3 or other similar fertility-related genes.

Particularly, the breeding of above-mentioned male sterile line and maintenance, refer to homozygous recessive kernel male sterile mutant for transformation receptor material, is converted in this sterile mutant recipient plant by closely linked 3 target genes.Described 3 target genes are fertility restorer gene, pollen inactivated gene and color mark screening-gene respectively.Wherein, fertility restorer gene can make sterile transformation receptor fertility restorer, pollen inactivated gene can make the pollen inactivation containing the foreign gene transformed, namely fertilizing ability is lost, screening-gene may be used for the sorting of transgenic seed and non-transgenic seed, the non-transgenic seed sorted out is used as sterile line and produces cross-fertilize seed, and transgenic seed is used as maintenance line to produce sterile line continuously, stably.

More specifically, according to one embodiment of present invention, with the recessive sterile fg3/fg3 mutant of paddy rice core for transformation receptor material, closely linked 3 target genes can be converted into this sterile line: wherein, fertility restorer gene OsFG3 can make transformation receptor fertility restorer; Pollen inactivated gene Zm-PA can make the pollen inactivation containing foreign gene, namely loses fertilizing ability; Fluorescence color selects gene RFP (r) for the sorting of transgenic seed and non-transgenic seed, and the non-transgenic seed sorted out is used as sterile line and produces cross-fertilize seed, and transgenic seed is used as maintenance line to be come stably to produce sterile line continuously.Because this technology utilizes biotechnology to produce non-transgenic product, solve the bottleneck problem faced in paddy rice cross breeding production of hybrid seeds process, i.e. low the and problem that is sterile line fertility instability in bilinear method (method detailed can consult PCT patent PCT/CN2013/086657) of three series resource utilization.

Of the present invention provided pollen-specific is expressed promotor and be can be used for specific expressed in pollen of foreign gene, thus avoid this foreign gene continuous expression adverse effect in its hetero-organization of plant, plant pollen can also be used for and grow the functional analysis of genes involved and qualification; Can be used for the establishment of male sterile line and restorer; And can be applicable in pollen abortion experiment, thus avoid being drifted about by plant transgene or pollen is escaped the Biosafety problem brought, significant to the creation of male sterility line of plants and restorer.

Present invention also offers the production method of a kind of plant, it comprises:

(1) expression cassette that second aspect present invention or the 5th aspect provide is built;

(2) expression cassette step (1) obtained imports vegetable cell;

(3) regenerate transgenic plant; With

(4) transgenic plant are selected; And

(5) optionally, the plant that obtains of amplification step (4) is to obtain offspring.

Transgenic plant of the present invention use plant biotechnology field method for transformation preparation known to the skilled.Any method can be used to recombinant expression vector to be transformed in vegetable cell, to produce transgenic plant of the present invention.Method for transformation can comprise directly and indirectly method for transformation.Suitable direct method comprises the DNA absorption of polyoxyethylene glycol induction, liposome-mediated conversion, uses particle gun importing, electroporation and microinjection, etc.In the specific embodiment of the present invention, the transformation technology that present invention uses based on edaphic bacillus (can see (1985) Science 225:1229 such as Horsch RB; White FF, Vectors for Gene Transfer in Higher Plants, Transgenic Plants, the 1st volume, Engineering and Utilization, Academic Press, 1993, pp.15-38; .Techniques for Gene Transfer, the Transgenic Plants such as Jenes B, the 1st volume, Engineering and Utilization, Academic Press, 1993, pp.128-143, etc.).Edaphic bacillus bacterial strain (such as Agrobacterium tumefaciems or Agrobacterium rhizogenes) comprises plasmid (Ti or Ri plasmid) and T-DNA element, described plasmid and element are transferred to plant after with Agrobacterium transfection, and T-DNA is integrated in the genome of vegetable cell.T-DNA can be positioned on Ri-plasmid or Ti-plasmid, or is included in independently in so-called binary vector.Agrobacterium-mediated method for transformation is described in such as.The agrobacterium-mediated the most applicable dicotyledons of conversion, but be also applicable to monocotyledons.The conversion of edaphic bacillus to plant is described in such as.Conversion can cause instantaneous or stable conversion and expression.Although nucleotide sequence of the present invention can be inserted into any plant and vegetable cell that fall into these broad variety, it is particularly useful for crop plants cell.

Compared with prior art, the present invention has following beneficial effect: the male sterile line that the invention provides a kind of pollen development gene and produce based on this transgenation, the stable fertility of this sterile line, not by environmental influence, can be recovered by wild-type transgenic.The sterile element being structure third generation cross-breeding system and providing necessity that this gene and this transgenation produce, the male sterile line that this transgenation produces, be used for producing cenospecies, for breaking through and improveing existing " three are " and " two are " hybridization technique is significant.

Embodiment

Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.

Embodiment 1, rice male sterility mutant (osfg3) screen

The acquisition of this mutant accounts for seed (M0) by the yellow China of EMS mutagenesis long-grained nonglutinous rice, and EMS mutagenesis concentration and time are 0.7%, 12 hours, from M0 for the solid rear mixed receipts of seed plant, obtain mutant library (M1).Be used for screening at seed maturity for the plant of seed from M1, by Phenotypic Observation, obtain sterile strain.Rice stub regeneration is cut in sterile strain, regeneration strain in generative phase with I2-KI staining examine pollen development and staining reaction.One of them mutant shows as non-pollen type sterile (no pollen), called after osfg3.

Embodiment 2, rice male sterility mutant (osfg3) genetic analysis

The sterile strain of osfg3 mutant and the yellow China of wild-type account for hybridization, and three hybrid Population F1 generation plant all show as and can educate.Again F1 generation is carried out selfing, F2 is for the segregation ratio with fertile plant sterile in plant close to 1:3 (table 1), and showing this sudden change is controlled by Recessive genes.

Table 1: rice male sterility mutant (osfg3) hybridizes F2 for segregation ratio

Embodiment 3, rice male sterility mutant (osfg3) reproductive organ phenotype analytical

Compared with wild-type, mutant plants grows normally, and the same period blooms.Lemma and glumelle size, form, the first transaction of a day's business size, the first transaction of a day's business time and wild-type do not have difference (Fig. 1).But mutant flower pesticide is modest, in white (Fig. 2), flower pesticide does not ftracture, and does not have pollen, carries out staining examine further with I2-KI solution to the pollen of mutant, and as shown in Figure 3, the pollen staining of wild-type is normal, and mutant does not have pollen for result.The female organ (comprising ovary, style, column cap) all bigger than wild-type (Fig. 4) of mutant.Mutant stigma exposure rate more than 85% (Fig. 5), and the yellow China of wild-type accounts for column cap and seldom exposes.Compared with accounting for the yellow China of wild-type, the trichome on mutant blade becomes many, and diminish (Fig. 6).Glumelle trichome compares, and compared with accounting for the yellow China of wild-type, the trichome on mutant glumelle becomes many, and diminish (Fig. 7).

The clone of embodiment 4, rice male sterility mutant gene

Mutant gene clone takes Mutmap method, namely utilizes the raw parents of mutant and open country to build F2 colony, is carried out the method for the assignment of genes gene mapping by sequence of resurveying.Sterile strain and the yellow China of wild-type are accounted for hybridization, choose 30 F2 for sterile plant, get blade and extract genomic dna, for high throughput genome sequencing after balanced mix, obtain about 18.5Gb genomic sequence data altogether, be equivalent to 43x rice genome (table 2).Account for genome sequence with the yellow China of wild-type and compare that to show mutator gene may be Os10g35180 allelotrope on the 10th karyomit(e).

Table 2 rice male sterility mutant is resurveyed order sequenced data

At the coding region total length 2181bp of yellow this gene of Hua Zhanzhong of wild-type, its nucleotide sequence is as shown in SEQ ID NO:1.Albumen coded by SEQ ID NO:1 is containing 727 amino acid, and its aminoacid sequence is as shown in SEQ ID NO:2.In sterile mutant, the shearing site of this gene the 8th and the 9th exon is by GT ... AG sports AT ... AG (Fig. 8), makes corresponding intron can not be sheared (Fig. 8), causes the premature termination of this gene.Adopt up-to-date SNP (single nucleotide polymorphism) research tool HRM (High Resolution Melt, namely high resolving power melts) analyze, the all WUHUAFEN plant of further checking all carries homozygous mutant site, and fertile plant carries pure and mild wild-type or heterozygous site (table 3).This site is that the plant self progeny of pure and mild wild-type all can educate, and this site is that the plant self progeny of heterozygous shows sterile: can educate 1:3 and be separated.

Table 3 mutator gene (Os10g35180) HRM genotyping result

Nucleotide sequence polymorphism (Fig. 8) is there is in this gene cDNA encoding district between the yellow China of warm and fine wild-type of japonica rice Japan accounts for, compared with Huang Huazhan, the T of the 834th being specially the fine nucleotide sequence at this gene of japonica rice Japan sports C, the C of the 1371st sports T, the A of the 1422nd sports G, the G of the 1515th sports A, the disappearance of an appearance fragment of 2298-2307 position, concrete deletion fragment is CGCCCGCGG, the G of the 2324th sports T, cause the polymorphism of aminoacid sequence at 696-698 position place's appearance 3 amino-acid residues of this corresponding gene, the amino-acid residue of the 705th sports Serine S (Fig. 9) by L-Ala A, it is concrete in japonica rice Japan is fine, the nucleotide sequence of this gene is as shown in SEQ ID NO:5, the aminoacid sequence of its coding is as shown in SEQ ID NO:6.Further analysis, this gene does not have polymorphism between rice variety 9311 and the yellow China of wild-type account for.

Embodiment 5, the OsFG3 gene expression analysis in each organ of paddy rice

According to the cDNA sequence design primer of OsFG3, upstream primer is F15 ' GGCTGATCACCTACTACAAGAACTC 3 ' (SEQ ID NO:24), downstream primer is R15 ' CTCCTGCATCCAGCACTGTC 3 ' (SEQ ID NO:25), simultaneously using paddy rice Actin gene as internal reference control design primer, upstream primer is 5 ' GCTATGTACGTCGCCATCCA ' (SEQ ID NO:26), and downstream primer is 5 ' GGACAGTGTGGCTGACACCAT ' (SEQ ID NO:27).Account for rice material with yellow China extract total serum IgE and synthesize cDNA template.Take real time quantitative PCR method, analyze OsFG3 gene in rice root, stem, leaf, lemma, glumelle, lepicena, gynoecium and young fringe grain husk flower primordium differentiation phase (stage6), children's fringe pollen mother cell Meiosis (stage7) and tetrad formation stages (stage8), sporule early stage (stage9), the express spectra of sporule middle and advanced stage (stage10) and mature pollen phase (stage12), its result as shown in Figure 10, this gene is at early stage (stage9) specifically expressing of sporule and expression amount is very high, start to reduce at sporule middle and advanced stage (stage10) expression amount, and at root, stem, leaf, seed and other panicle developmental stage expression level very low.The expression of this gene gynoecium, young fringe pollen mother cell Meiosis (stage7) and tetrad formation stages (stage8), sporule early stage (stage9) and sporule middle and advanced stage (stage10) in mutant is obvious down regulation trend (Figure 11).

The promoter expression vector of embodiment 6, OsFG3 gene builds and functional analysis

The structure of the promoter expression vector (Figure 12) of OsFG3 gene: with primer OsFG3-Pro-F (ACTGGTACCCTTAATCCCTTCTATACC, SEQ ID NO:28) and OsFG3-Pro-R (TCTGTCGACTCATCTCTCCCTCTCTCGC, SEQ ID NO:29) amplifying rice genome obtains the OsFG3 promoter gene fragment (SEQ ID NO:3) of 2010bp, obtains pOsfg3-gus carrier by being connected in promoter detection carrier pHPG after this fragment Kpn1 and Sal 1 double digestion.Be converted in wild rice callus by agriculture bacillus mediated method, screening regeneration obtains transfer-gen plant.

Screening is regenerated the transgenic rice plant obtained, by analyzing the expression pattern of the activation analysis OsFG3 promotor of beta-galactosidase, the root of genetically modified plant, stem, leaf and in spending by GUS staining analysis, find that OsFG3 promoters driven GUS mainly expresses in paddy pollen.In addition, promotor SEQ ID NO:9 is connected GUS and carries out functional verification discovery, its coloration result is consistent with the result of SEQ ID NO:3, is the promotor of flower pesticide specifically expressing type.

The transgenic function complementation of embodiment 7, rice male sterility mutant (osfg3)

The structure (Figure 13) of complementing vector: with primer Osfg3-Res-F (ACTGGTACCCTTAATCCCTTCTATACC, SEQ ID NO:30) and Osfg3-Res-R (AGTGGATCCGGATCATGGAAATATCAAGTAC, SEQ ID NO:31) amplifying rice genome obtains the full length sequence fragment (SEQ ID NO:4) of 748 bases after the base of about 2010bp containing Osfg3 initiator codon ATG upstream and terminator codon TGA, pOsfg3-complementing vector is obtained by being connected in genome complementation expression vector after this fragment Kpn1 and BamHI double digestion.Being converted into Huang Hua by agriculture bacillus mediated method accounts in the callus of the Seed inducement of osfg3 mutant, and screening regeneration obtains transfer-gen plant.

Analyze the transgenic positive plant of all acquisitions, find that all transgenic positive plant all show and can educate.These are analyzed proves that gene OsFG3 participates in pollen development regulation and control further, and this transgenation causes WUHUAFEN phenotype.

The acquisition of the transgenic line of the RNAi interference of embodiment 8, OsFG3 gene and phenotype analytical

The structure of the RNAi interference carrier (Figure 14) of OsFG3 gene: with primer Osfg3-Flag-F1 (ctcagatctGATCCAAGTGGCTGGACCTC, SEQ ID NO:32) and Osfg3-Flag-R1 (ctcactagtCAGCTGCTGATATACACCTTGA, SEQ ID NO:33) amplifying rice cDNA obtains the OsFG3 gene fragment of 244bp, forward and reverse OsFG3 gene fragment 1 (SEQ ID NO:34) and OsFG3 gene fragment 2 (SEQ ID NO:35) is obtained respectively with two pairs of amplimer amplifications with Bgl2 and Spe1 restriction enzyme site, enzyme is cut to be connected in pRNAi carrier and is obtained pOsfg3-RNAi 1.Also construct another one RNAi carrier simultaneously, with primer Osfg3-Flag-F2 (ctcagatctGATCCAAGTGGCTGGACCTC, SEQ ID NO:36), Osfg3-Flag-R2 (ctcactagtCAGCTGCTGATATACACCTTGA, SEQ ID NO:37) amplifying rice cDNA obtains the OsFG3 gene fragment of 480bp, forward and reverse OsFG3 gene fragment 3 (SEQ ID NO:38) and OsFG3 gene fragment 4 (SEQ ID NO:39) is obtained respectively with two pairs of amplimer amplifications with Bgl2 and Spe1 restriction enzyme site, enzyme is cut to be connected in pRNAi carrier and is obtained pOsfg3-RNAi2.Transform the fine callus of Japan by agriculture bacillus mediated method, screening regeneration obtains transfer-gen plant.

The sequence alignment of the homologous protein predicted in the albumen of embodiment 9, OsFG3 genes encoding and barley, Chinese sorghum, Maize genome

In ncbi database, utilize protein blast instrument, the albumen complete sequence of rice Os FG3 genes encoding is searched in albumen database, obtain barley (Hordeum vulgare), Chinese sorghum (Sorghum bicolor), millet (millet) (Setaria italica), two fringe false bromegrasses (Brachypodium distachyon), the homologous protein predicted in the genome of corn (Zea mays), these protein sequences are compared, result display all has closely similar conserved sequence from the homologous protein of different plant, homology very high (Figure 15) each other, show that this albumen biological function in the male organs growth course of plant flowers is guarded, play very important effect.

Wherein, in barley, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:10 or 11, and its aminoacid sequence is as shown in SEQ ID NO:12; In Chinese sorghum, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:13 or 14, and its aminoacid sequence is as shown in SEQ ID NO:15; In corn, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:16 or 17, and its aminoacid sequence is as shown in SEQ ID NO:18; In millet, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:19, and its aminoacid sequence is as shown in SEQ ID NO:20; In two fringe false bromegrasses, the nucleotide sequence of this fertile gene is as shown in SEQ ID NO:21 or 22, and its aminoacid sequence is as shown in SEQ ID NO:23.

SEQUENCE LISTING

<110> Shenzhen Crop Molecular Design Breeding Institute

Shenzhen Xingwang Biological Seed Industry Co., Ltd.

Xingwang Investment Pty Ltd.

 

<120> sterility changing gene FG3 and application thereof

<130>

<160> 39

<170> PatentIn version 3.3

<210> 1

<211> 2181

<212> DNA

<213> paddy rice (Oryza sativa Indica)

<400> 1

atgaagaagc agggaggagg aggaggagta gcggcggcgg cggtggcggc ggcggggatg 60

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tcggcgcgcc tggcgtggcg cgacctcacc gtcaccgtcg tgctcggcgg cggcggcggc 240

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ctcgccggcc gcctcgccgc caacgccttc ctcgacggca ccgtcctcct caacggccgc 420

aaggccaacc tctccttcgg cgccgcggcc tatgtgaccc aagatgacaa cttgatcggc 480

acattgacgg tgagggagac catctcatac tcggcgcggc ttcgccttcc cgacaagatg 540

cccatggaag agaagcgcgc ccttgtcgag ggcacaatcg tcgagatggg gctgcaggac 600

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agggtcagca ttgcccttga gatactgatg aggcccaggc tgctcttcct ggatgaacca 720

accagtggcc ttgacagtgc ttcagcgttc ttcgtgacac agacactgcg tgggctagcg 780

agggatggca ggacagtgat agcatcgatc catcagccga gcagcgaggt ttttgagctt 840

ttcgaccgct tgtatctcct ttcaggaggc aaaactgttt actttgggct ggcctctgaa 900

gcttgtcagt tctttgccca agccggcttc ccttgcccac cgctgcgcaa tccatcggat 960

catttcctca gatgcataaa cgcagacttc gacaaggtga aggccactct gaaaggatca 1020

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ttttggatgc aggctttcac actcacgaag cgatcgttca tcaacatgtc gcgggacttc 1260

gggtactact ggctgaggct tatcatctac attgttgtga cagtctgcat cgggacaatc 1320

tacctcaatg ttggcaccag atacagctca attctggcaa gaggtgcatg cgcttctttc 1380

gtcttcggct ttgtcacgtt catgtcgatt ggaggattcc catcttttgt ggaagacatg 1440

aaggtgtttc agagagagag gctcaatggg cactatggcg tgctggcatt cgtgataagc 1500

aacacgatct cggcgatgcc attcttgatc ttgatcacct tcatctccgg gacaatgtgc 1560

tacttcatgg tgcgcctcca cccgggcttc acgcactacc tcttcttcgt cctctgcctc 1620

tacgcaagcg tcaccgtcgt cgagagcttg atgatggcca ttgccagtgt catccccaac 1680

ttcctcatgg gcatcatcat cggtgcagga attcagggga tattcatgct ggtttcagga 1740

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cagatcgacg tgagccgatc caagtggctg gacctcgccg tgctcttcag catgatcttc 1980

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cgcggctacg tcgcgcggcg ccgggtgcag ggcaagggcg cccgcggcgc ccgcggccgc 2100

ggggcggacc tcgccgccgc gcgctcgccg tcgctccgcg cctacgtcgt cgacgccgcc 2160

gacgacctgc cgccggcctg a 2181

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Ala Trp Arg Asp Leu Thr Val Thr Val Val Leu Gly Gly Gly Gly Gly

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Gly Lys Ser Thr Leu Leu Asp Ala Leu Ala Gly Arg Leu Ala Ala Asn

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Ala Phe Leu Asp Gly Thr Val Leu Leu Asn Gly Arg Lys Ala Asn Leu

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Ser Phe Gly Ala Ala Ala Tyr Val Thr Gln Asp Asp Asn Leu Ile Gly

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Thr Leu Thr Val Arg Glu Thr Ile Ser Tyr Ser Ala Arg Leu Arg Leu

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Pro Asp Lys Met Pro Met Glu Glu Lys Arg Ala Leu Val Glu Gly Thr

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Pro Ser Ser Glu Val Phe Glu Leu Phe Asp Arg Leu Tyr Leu Leu Ser

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His Phe Leu Arg Cys Ile Asn Ala Asp Phe Asp Lys Val Lys Ala Thr

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Leu Lys Gly Ser Met Lys Arg Arg Phe Glu Arg Ser Asp Asp Pro Leu

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Asp Arg Ile Met Thr Ser Glu Ala Ile Arg Arg Leu Ile Thr Tyr Tyr

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Lys Asn Ser Gln Tyr Tyr Phe Ala Ala Gln Gln Lys Val Asn Glu Met

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Ala Arg Val Lys Gly Thr Val Leu Asp Ala Gly Gly Ser Gln Ala Ser

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Phe Trp Met Gln Ala Phe Thr Leu Thr Lys Arg Ser Phe Ile Asn Met

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Ser Arg Asp Phe Gly Tyr Tyr Trp Leu Arg Leu Ile Ile Tyr Ile Val

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Val Thr Val Cys Ile Gly Thr Ile Tyr Leu Asn Val Gly Thr Arg Tyr

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Ser Ser Ile Leu Ala Arg Gly Ala Cys Ala Ser Phe Val Phe Gly Phe

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Val Thr Phe Met Ser Ile Gly Gly Phe Pro Ser Phe Val Glu Asp Met

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Thr Val Val Glu Ser Leu Met Met Ala Ile Ala Ser Val Ile Pro Asn

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Val Gln Gly Lys Gly Ala Arg Gly Ala Arg Gly Arg Gly Ala Asp Leu

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Ala Ala Ala Arg Ser Pro Ser Leu Arg Ala Tyr Val Val Asp Ala Ala

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cttaatccct tctatacctc tgaattttat ctcatcctgt tttcaaattt acgatcaagt 60

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aattttaaaa gcttaactga atcttgacct aaacattaaa tatttatctc attctaatcg 420

atgttaacaa tttctcaact tttgctataa aatcttaatc gcacgtaaat gataggcacc 480

attgcactag gtgtaaaata aaaaccttat cctctactcc ctcggcccca aaataagttt 540

attttcaccc atctcatata taataatagt aataataata ataataataa taataataat 600

aataataata ataataataa taataataat aatataatac ccccacctta tcaaatctca 660

atgcaattat ttctctcttt atccaccccc aatgcattta tccactgctt tcagaaactt 720

tgatttaatg atagctaaaa ataaacttat tttaggataa ttaaaaacac gaatatcagg 780

gaaggaccta ttatcaaata attagaaggg gtgaggcttc gaacccaggt cgtctagccc 840

acaccacctt gtggagctag ctggaaaacc ctgggcattt ctcttatttt aggataactg 900

aaaataaacc ataaatgata ttattctgaa agagggaata ctctcttttg caaatgaaaa 960

aaaaaaacta taacaagaaa ccatggtcgg gatagcattt gtgcagcaga aagaacctaa 1020

accgtgtgtg gctgcattgc atgcatgata gtgatgagat accatatggc tgttcacaac 1080

ccaacaactt gcaatccagt gcatgcatta catggccaag aatccagcgg ttgagccact 1140

gacatgtata ttccactttc gttatgaccc gcatgtcagt ggctcaattg cacatacagc 1200

taagtcagag aatttccttc atctctacca atctattctc tctctttggc ctggtttagt 1260

cccaaaacat catatcgaat ctttgaagac atgcatgtag cattaaatat ataataaaaa 1320

aaactaattg catagttagg cggaaatcac gagacgaatc ttttgagcct aataagtcca 1380

ttattagccg taagtgctac agtaacccac atacgctaat gacggattaa ttaagctcaa 1440

aagattcgtc ttgcgatttt caggcaagtt atgaaattag ttttttcatt cgtgtccgaa 1500

aacccctacc gatatccagt caaacatccg atgtgacacc caaacatttc ttttcttgaa 1560

ttaaacatgc cctttctctt gtgcttgcat tgcatatgca tatgcacaat gcattgcttc 1620

tagatcacac caaacagcta ctagcccccc cttatgacat gccattcatc tccattgcat 1680

gcttgcatat tgcatgcatg ctgctgctgc ttctactcct ccaactctct agagctagct 1740

accttgaccc taggtcaaac ccctacaccc ctcattactt ctgcacctga tctccttctc 1800

tctctagctt ctctccctag attttctctc tctaacctgc tcctccatta ctggccttta 1860

tatccaccac ctcctcctct tctcctccct cataggtggt gtttgcttag ctagctagct 1920

agctccatca ctagctctcg catcgcatgc cacggtgagc cggtgtcgcc ggcgtcgtac 1980

ggacgtagca agcgagagag ggagagatga 2010

<210> 4

<211> 8024

<212> DNA

<213> paddy rice (Oryza sativa Indica)

<400> 4

ccttaatccc ttctatacct ctgaatttta tctcatcctg ttttcaaatt tacaatcaag 60

taactatatt atgtttgttt ttttaaaaat aaatcatttt tttgttaagg gctgtctttt 120

tcaatcaact tgcacacaag tacactaaca gacaagggta atatggatat ttttacaaaa 180

atctaatggt taactaacta tcgactcacg gaatagacat ggaagggatc aaaataaata 240

aaaactcaag gctatatagg ggatgaggct aaatttaggg gcacataagg gaattggcga 300

aaattcgtgg acataaggaa ttttatattt tataaaactt attgtaatat attattttgt 360

ttttagaatt ttaaaagctt aactgaatct tgacctaaac attaaatatt tatctcattc 420

taatcgatgt taacaatttc tcaacttttg ctataaaatc ttaatcgcac gtaaatgata 480

ggcaccattg cactaggtgt aaaataaaaa ccttatcctc tactccctct gccccaaaat 540

aagtttattt tcacccatct catatataat aataataata ataataataa taataataat 600

aataataata ataataataa taataccccc accttatcaa atctcaatgc aattatttct 660

ctttatccac ccccaatgca tttatccact gctttcagaa actttgattt aatgatagct 720

aaaaataaac ttattttagg ataattaaaa acacgaatat cagggaagga cctattatca 780

aataattaga aggggtgagg cttcgaaccc aggttgtcta gcccatacca ccttgtggag 840

ctagctggaa aaccctgggc atttctctta ttttaggata actgaaaata aaccataaat 900

gatattattc tgaaagaggg aatactctct tctgcaaatg aaaaaaaaaa actataacaa 960

gaaaccatga tcgggatagc atttgtgcag cagaaagaac ctaaaccgtg tgtggctgca 1020

ttgcatgcat gatagtgatg agataccata tggctgttca caacccaaca acttgcaatc 1080

cagtgcatgc attacatggc caagaatcca gcggttgagc cactgacatg tatattccac 1140

tttcgttatg acccgcatgt cagtggctca attgtacgta cagctaagtc agagaatttc 1200

cttcatctct accaatctat tctctctctt tggcctggtt tagtcccaaa acatcatatc 1260

gaatctttga aaacatgcat gtagcattaa atatataata aaaaaaacta attgcatagt 1320

taggcggaaa tcacgagaca aatcttttga gcctaattag tccattatta gccgtaagtg 1380

ctacagtaac ccacatacgc taatgacgga ttaattaagc tcaaaagatt cgtcttgtga 1440

ttttcaggca agttatgaaa ttagtttttt cattcgtgtc cgaaaacccc taccgatatc 1500

cagtcaaaca tccgatgtga cacccaaaca tttcttttct tgaattaaac atgccctttc 1560

tcttgtgctt gcattgcata tgcatatgca caatgcattg cttctagatc acaccaaaca 1620

gctactagcc cccccttatg acatgccatt catctccatt gcatgcttgc atattgcatg 1680

catgctgctg ctgcttctac tcctcctcct ctctagagct agctaccttg accctaggtc 1740

aaacccctac acccttcatt acttctgcac ctgatctcct tctctctcta gcttctctcc 1800

ctagattttc tctctctaac ctgctcctcc attactggcc tttatatcca ccacctcctc 1860

ctcttctcct ccctcatagg tggtgtttgc ttagctagct agctagctcc atcactagct 1920

ctcgcatcgc atgccacggt gagccggtgt cgccggcgtc gtacggacgt agcaagcgag 1980

agagggagag atgaagaagc agggaggagg aggaggagta gcggcggcgg cggtggcggc 2040

ggcggggatg gtgaaggcgg agctggagga cgtggggatc aaggcggcgg gaggggcggt 2100

ggcggcgctg agcccgctga gcgagacgct gtggcgggag aaggcggcgg cggagttcct 2160

cggcgacgtg tcggcgcgcc tggcgtggcg cgacctcacc gtcaccgtcg tgctcggcgg 2220

cggcggcggc ggcggcggag gagggacgac gcaggacgtg ctccaggggc tcacggggca 2280

cgccgagccg ggcaccatca cggcgctcat gggcccctcc ggctccggca agtccaccct 2340

gctcgacgcc ctcgccggcc gcctcgccgc caacgccttc ctcgacggca ccgtcctcct 2400

caacggccgc aaggccaacc tctccttcgg cgccgcggta cgcacgcaca cctcgccgcc 2460

ggcgacccgt cgccactgtg ctgaactgct gaccgcaacg ctaagttaat cagttattag 2520

tagtagtaca atagtactaa atactagtaa atactaatca gctttgcttt tatatgggat 2580

tggattttgc ttcagaaaat ggttttctgg attaaaagct gcacattaaa ctaaagaagt 2640

actccctccg tttcacgatg taaatcattc tgtcatttcg cacattcata ttgatgttaa 2700

tgaatctaaa catatataca tcaatattaa tatgggaaaa gttagaatga tttacattgt 2760

gaaacggagg aagtagttca tttcttgaag tataactgca cacagggaga atacttgtag 2820

tacaaaagaa aaaaaaacat ggaatatgtg tgtgttcttt ttgcagtact tcattttctt 2880

gaagaagatt tatttttttt tgtgtgtgtt tgattagaat gtgtattgta tatttttttt 2940

tcctttctgt tgctttcatt acttggtctt tatctgaatc acctgaacaa ccgatttcag 3000

aatgagaaag aaatgggtca tttttttgtt gttgccacgt gccatttctt ggaaccttgc 3060

tgctttctgg cttctgcatg tgtttggaca gactgcctct ctgcagatgt aatttactgc 3120

attaataact ccctttcatg gatatagatc acccatttct ggccaagatt aattggggga 3180

tctggctcaa tcaattaatt agtttctgct tattaatttt tagataggaa aaaaaaaagc 3240

tctttcaggt atgtgtttgg taatggagtt aacactcatt cagtcatcta gcttgttggc 3300

aaggaaaact aggaacttcc ccagcagatg ggatgtggtt taccagtttt ttcagaaact 3360

atgctcatca aaaactcatg atctgttgac aatcctgatt atttgttgta catgtagcgt 3420

gtgtcgccat gttgtccaaa atcatttctt tatgacttaa caggagcttt tttaaaagaa 3480

aaaacatagt ccaaaagaag gttgttgctg atatttttca tgacttgtgc agttgtgctt 3540

catttgtttc tgtcaaaact gtcaacctaa tatgtaggaa gtagtttaac ttctgtacca 3600

atcaataact ataaagtatt atcaaggaaa aaaagagcaa aatttctgta tgtaaaaagt 3660

tctaccttgt caaaaatgat aaggtgatac ctgaagttat gtgcatatat tatcttactt 3720

cgtctgatgc agtaataatc tcaaggtaac aaaagaagtt acctccaggc tccagcttac 3780

tgaaagtttc agtaaaatat gtaatttcag gcctatgtga cccaagatga caacttgatc 3840

ggcacattga cggtgaggga gaccatctca tactcggcgc ggcttcgcct tcccgacaag 3900

atgcccatgg aagagaagcg cgcccttgtc gagggcacaa tcgtcgagat ggggctgcag 3960

gactgcgccg acacggtggt cggcaattgg cacctgaggg gaatcagtgg tggtgagaag 4020

aggagggtca gcattgccct tgagatactg atgaggccca ggctgctctt cctggatgaa 4080

ccaaccagtg gccttgacag gtaggtactg ctattatatt tatggcaaat catttgttat 4140

tacattaatt aatggatcat gcaagcatgc cgggtcggcc aaaggctggc cgaccaattt 4200

cattaagatt gggagaaaat aaagtaaaca tttagttaca tccaggccca gcatgatagc 4260

aatctagcta tctagcaagc atgatagcaa tctagctgaa cttgagatgt ggagatttga 4320

tttgcacagc tctctacctt gctttgcttt caaccagtag tgtttatcaa actcattcct 4380

ggtataacta tcagaaggtt actatgcctt gtcatttgtc aagtgattgt aattgttaca 4440

gtagcaatta tctgtctaag taactgatgt gcagatgatt aatctgttgc agtgcttcag 4500

cgttcttcgt gacacagaca ctgcgtgggc tagcgaggga tggcaggaca gtgatagcat 4560

cgatccatca gccgagcagc gaggtttttg agcttttcga ccgcttgtat ctcctttcag 4620

gaggcaaaac tgtttacttt gggctggcct ctgaagcttg tcaggtatgt cagttttcac 4680

aaactctatt aagttgtttc tgcaatgcta atatctatat gggttccctc aatcatgtac 4740

tgttctttgc attttgcagt tctttgccca agccggcttc ccttgcccac cgctgcgcaa 4800

tccatcggat catttcctca gatgcataaa cgcagacttc gacaaggtga aggccactct 4860

gaaaggatca atgaagagaa gagtaagttt caggattcag tcagtctcct cctagtacct 4920

acaactggca ctcttacctg tgcatgcctt gactgaaatt gtgctcacat tttgcataca 4980

tagtttgaaa gatccgatga cccacttgat cggattatga cttcggaggc catcagaagg 5040

ctgatcacct actacaagaa ctcacagtac tactttgctg cgcagcagaa agtcaatgag 5100

atggcacggg ttgtaagtaa ctagattact acagcaactt ccccaaacat tcaaccacag 5160

ctagaccatt gtaatgcaag ctaagaggac tcacatggta tttttgttgc agaaagggac 5220

agtgctggat gcaggaggga gccaggctag cttttggatg caggctttca cactcacgaa 5280

gcgatcgttc atcaacatgt cgcgggactt cgggtactac tggctgaggc ttatcatcta 5340

cattgttgtg acagtctgca tcgggacaat ctacctcaat gttggcacca gatacagctc 5400

aattctggta aaaatttcct tcttcagttg tcacttctat ttggatgcaa acttacactg 5460

aaaagtatct agctaccctg aactgaatac ttgatgttaa gcttggtgat ctgataaaac 5520

tgtgaaaaca attaggcaag aggtgcatgc gcttctttcg tcttcggctt tgtcacgttc 5580

atgtcgattg gaggattccc atcttttgtg gaagacatga aggtttgtgt caccactgaa 5640

tcaacaaatg ggttagaaaa gttcttcaga ttacagcaaa ccatgctcac agaatctttt 5700

ttatgctctt tcaggtgttt cagagagaga ggctcaatgg gcactatggc gtgctggcat 5760

tcgtgataag caacacgatc tcggcgatgc cattcttgat cttgatcacc ttcatctccg 5820

ggacaatgtg ctacttcatg gtgcgcctcc acccgggctt cacgcactac ctcttcttcg 5880

tcctctgcct ctacgcaagc gtcaccgtcg tcgagagctt gatgatggcc attgccagtg 5940

tcatccccaa cttcctcatg ggcatcatca tcggtgcagg aattcaggtg aattaattac 6000

catccatcac aacaacttga tcctgtccat ctttatcagg cactgtttgg agtcctgcaa 6060

cgcatacaaa ctgggattaa attttgacac tgtacttgga actgcacaac ataatgttga 6120

tgtttgttaa tattctctca tgatcatgac atgcaacaac atttattgaa gagaaaaata 6180

tattttggaa atggacaggg gatattcatg ctggtttcag gatacttcag gcttccccat 6240

gacatcccaa agccattctg gaggtatccc atgtcataca tcagcttcca ctactgggca 6300

ttgcaggtaa aaatttcaac tttttttaca gctcataaac tgaagcaacc agtgaactca 6360

aatcagggtt taaaacaaca aatcaggaaa atttaaaccc aagaccaaaa aatcaaaatc 6420

aaaattttgt ccatgtgtta ctgctgttac ttctgttgtc acaagtacat gtatagaaca 6480

tgacatgaga aacaacgaat ttgagaaatt tgaagcaaaa atgggccaat ttcaatcttg 6540

acaggtttcg tcgatatttt ccataatttt tgtcaatctc ttaaactggt tatacctctg 6600

ttgtcagttt tcactagtat atgtaatact agaacataaa acaactattg ataattatac 6660

atgaggaaca ataaattcgg aaaaactacc agcaaaaaag cggcaaattt gaattttgat 6720

gggtttgatc gatatttttc ataatttttg tcaatctctt aaactgctta ttatacatct 6780

gttatcagct ttcaccacca catgtaatag aacataaaac aactatggag aattacacac 6840

gagaagcaac aaattcaagc aaaaaatgtg cagattcgaa tgtttgactg gttttttttt 6900

tccatctgac attgttgcag ggtcagtacc agaacgacct gaagggcctg gtgttcgaca 6960

accaggacga cgagctcccc aagatccccg gcgagtacat cctggagaac gtgttccaga 7020

tcgacgtgag ccgatccaag tggctggacc tcgccgtgct cttcagcatg atcttcatct 7080

accgcctcct cttcttcgcc atgatcaagg tcagcgagga cgtcaccccg tgggtgcgcg 7140

gctacgtcgc gcggcgccgg gtgcagggca agggcgcccg cggcgcccgc ggccgcgggg 7200

cggacctcgc cgccgcgcgc tcgccgtcgc tccgcgccta cgtcgtcgac gccgccgacg 7260

acctgccgcc ggcctgacca cccgtgagac agacacggct tctgaaaact ctgaaactct 7320

gaactcctca actgaacaag tggagacaga ggaagaaaaa gagagagagg gagaggctaa 7380

aactgaacta aaaaaaaaag aacctgaata tctgatatga taattgtgtg ttatagtagt 7440

agcaatgctt aattaatcga tcaagctgtg ttgttgtatt gtattgataa ttactaatat 7500

caaggtgtat atcagcagct gaatgcaagt ttagctagtg cttagtttca ggtctctgtc 7560

ttatctctga tcttgctgca gttagtagta acaacttatt caagttggaa atttgatggt 7620

tccattttgt ttggatggca tcttgcagca gtgggtttgt ccaaaatgga gctacagctg 7680

ctacagattg gcaatgatgc atatggcagc aaggtactga agattttcac aaggatcttg 7740

tcaggacagt atgcagtttg gttgtgcaag agaaaaacat ccaagaaagg acatatgata 7800

tttatgagta ttgaagagca aactgttcgc tacttgcttg taccatgatc catcatttag 7860

tctaaacaca ttgcaagatg gtgtggcctg ttaaagaagc aattttgcaa tgttgataag 7920

taattaatga caagctgttt tatcatgtga ggagagttta tttgtatgca gacaagagca 7980

acaaactgta tatatgctag tagtacttga tatttccatg atcc 8024

<210> 5

<211> 2172

<212> DNA

<213> paddy rice (Oryza sativa Japonica)

<400> 5

atgaagaagc agggaggagg aggaggagta gcggcggcgg cggtggcggc ggcggggatg 60

gtgaaggcgg agctggagga cgtggggatc aaggcggcgg gaggggcggt ggcggcgctg 120

agcccgctga gcgagacgct gtggcgggag aaggcggcgg cggagttcct cggcgacgtg 180

tcggcgcgcc tggcgtggcg cgacctcacc gtcaccgtcg tgctcggcgg cggcggcggc 240

ggcggcggag gagggacgac gcaggacgtg ctccaggggc tcacggggca cgccgagccg 300

ggcaccatca cggcgctcat gggcccctcc ggctccggca agtccaccct gctcgacgcc 360

ctcgccggcc gcctcgccgc caacgccttc ctcgacggca ccgtcctcct caacggccgc 420

aaggccaacc tctccttcgg cgccgcggcc tatgtgaccc aagatgacaa cttgatcggc 480

acattgacgg tgagggagac catctcatac tcggcgcggc ttcgccttcc cgacaagatg 540

cccatggaag agaagcgcgc ccttgtcgag ggcacaatcg tcgagatggg gctgcaggac 600

tgcgccgaca cggtggtcgg caattggcac ctgaggggaa tcagtggtgg tgagaagagg 660

agggtcagca ttgcccttga gatactgatg aggcccaggc tgctcttcct ggatgaacca 720

accagtggcc ttgacagtgc ttcagcgttc ttcgtgacac agacactgcg tgggctagcg 780

agggatggca ggacagtgat agcatcgatc catcagccga gcagcgaggt tttcgagctt 840

ttcgaccgct tgtatctcct ttcaggaggc aaaactgttt actttgggct ggcctctgaa 900

gcttgtcagt tctttgccca agccggcttc ccttgcccac cgctgcgcaa tccatcggat 960

catttcctca gatgcataaa cgcagacttc gacaaggtga aggccactct gaaaggatca 1020

atgaagagaa gatttgaaag atccgatgac ccacttgatc ggattatgac ttcggaggcc 1080

atcagaaggc tgatcaccta ctacaagaac tcacagtact actttgctgc gcagcagaaa 1140

gtcaatgaga tggcacgggt taaagggaca gtgctggatg caggagggag ccaggctagc 1200

ttttggatgc aggctttcac actcacgaag cgatcgttca tcaacatgtc gcgggacttc 1260

gggtactact ggctgaggct tatcatctac attgttgtga cagtctgcat cgggacaatc 1320

tacctcaatg ttggcaccag atacagctca attctggcaa gaggtgcatg tgcttctttc 1380

gtcttcggct ttgtcacgtt catgtcgatt ggaggattcc cgtcttttgt ggaagacatg 1440

aaggtgtttc agagagagag gctcaatggg cactatggcg tgctggcatt cgtgataagc 1500

aacacgatct cggcaatgcc attcttgatc ttgatcacct tcatctccgg gacaatgtgc 1560

tacttcatgg tgcgcctcca cccgggcttc acgcactacc tcttcttcgt cctctgcctc 1620

tacgcaagcg tcaccgtcgt cgagagcttg atgatggcca ttgccagtgt catccccaac 1680

ttcctcatgg gcatcatcat cggtgcagga attcagggga tattcatgct ggtttcagga 1740

tacttcaggc ttccccatga catcccaaag ccattctgga ggtatcccat gtcatacatc 1800

agcttccact actgggcatt gcagggtcag taccagaacg acctgaaggg cctggtgttc 1860

gacaaccagg acgacgagct ccccaagatc cccggcgagt acatcctgga gaacgtgttc 1920

cagatcgacg tgagccgatc caagtggctg gacctcgccg tgctcttcag catgatcttc 1980

atctaccgcc tcctcttctt cgccatgatc aaggtcagcg aggacgtcac cccgtgggtg 2040

cgcggctacg tcgcgcggcg ccgggtgcag ggcaagggcg cccgcggccg cggggcggac 2100

ctctccgccg cgcgctcgcc gtcgctccgc gcctacgtcg tcgacgccgc cgacgacctg 2160

ccgccggcct ga 2172

<210> 6

<211> 723

<212> PRT

<213> paddy rice (Oryza sativa Japonica)

<400> 6

Met Lys Lys Gln Gly Gly Gly Gly Gly Val Ala Ala Ala Ala Val Ala

1 5 10 15

Ala Ala Gly Met Val Lys Ala Glu Leu Glu Asp Val Gly Ile Lys Ala

20 25 30

Ala Gly Gly Ala Val Ala Ala Leu Ser Pro Leu Ser Glu Thr Leu Trp

35 40 45

Arg Glu Lys Ala Ala Ala Glu Phe Leu Gly Asp Val Ser Ala Arg Leu

50 55 60

Ala Trp Arg Asp Leu Thr Val Thr Val Val Leu Gly Gly Gly Gly Gly

65 70 75 80

Gly Gly Gly Gly Gly Thr Thr Gln Asp Val Leu Gln Gly Leu Thr Gly

85 90 95

His Ala Glu Pro Gly Thr Ile Thr Ala Leu Met Gly Pro Ser Gly Ser

100 105 110

Gly Lys Ser Thr Leu Leu Asp Ala Leu Ala Gly Arg Leu Ala Ala Asn

115 120 125

Ala Phe Leu Asp Gly Thr Val Leu Leu Asn Gly Arg Lys Ala Asn Leu

130 135 140

Ser Phe Gly Ala Ala Ala Tyr Val Thr Gln Asp Asp Asn Leu Ile Gly

145 150 155 160

Thr Leu Thr Val Arg Glu Thr Ile Ser Tyr Ser Ala Arg Leu Arg Leu

165 170 175

Pro Asp Lys Met Pro Met Glu Glu Lys Arg Ala Leu Val Glu Gly Thr

180 185 190

Ile Val Glu Met Gly Leu Gln Asp Cys Ala Asp Thr Val Val Gly Asn

195 200 205

Trp His Leu Arg Gly Ile Ser Gly Gly Glu Lys Arg Arg Val Ser Ile

210 215 220

Ala Leu Glu Ile Leu Met Arg Pro Arg Leu Leu Phe Leu Asp Glu Pro

225 230 235 240

Thr Ser Gly Leu Asp Ser Ala Ser Ala Phe Phe Val Thr Gln Thr Leu

245 250 255

Arg Gly Leu Ala Arg Asp Gly Arg Thr Val Ile Ala Ser Ile His Gln

260 265 270

Pro Ser Ser Glu Val Phe Glu Leu Phe Asp Arg Leu Tyr Leu Leu Ser

275 280 285

Gly Gly Lys Thr Val Tyr Phe Gly Leu Ala Ser Glu Ala Cys Gln Phe

290 295 300

Phe Ala Gln Ala Gly Phe Pro Cys Pro Pro Leu Arg Asn Pro Ser Asp

305 310 315 320

His Phe Leu Arg Cys Ile Asn Ala Asp Phe Asp Lys Val Lys Ala Thr

325 330 335

Leu Lys Gly Ser Met Lys Arg Arg Phe Glu Arg Ser Asp Asp Pro Leu

340 345 350

Asp Arg Ile Met Thr Ser Glu Ala Ile Arg Arg Leu Ile Thr Tyr Tyr

355 360 365

Lys Asn Ser Gln Tyr Tyr Phe Ala Ala Gln Gln Lys Val Asn Glu Met

370 375 380

Ala Arg Val Lys Gly Thr Val Leu Asp Ala Gly Gly Ser Gln Ala Ser

385 390 395 400

Phe Trp Met Gln Ala Phe Thr Leu Thr Lys Arg Ser Phe Ile Asn Met

405 410 415

Ser Arg Asp Phe Gly Tyr Tyr Trp Leu Arg Leu Ile Ile Tyr Ile Val

420 425 430

Val Thr Val Cys Ile Gly Thr Ile Tyr Leu Asn Val Gly Thr Arg Tyr

435 440 445

Ser Ser Ile Leu Ala Arg Gly Ala Cys Ala Ser Phe Val Phe Gly Phe

450 455 460

Val Thr Phe Met Ser Ile Gly Gly Phe Pro Ser Phe Val Glu Asp Met

465 470 475 480

Lys Val Phe Gln Arg Glu Arg Leu Asn Gly His Tyr Gly Val Leu Ala

485 490 495

Phe Val Ile Ser Asn Thr Ile Ser Ala Met Pro Phe Leu Ile Leu Ile

500 505 510

Thr Phe Ile Ser Gly Thr Met Cys Tyr Phe Met Val Arg Leu His Pro

515 520 525

Gly Phe Thr His Tyr Leu Phe Phe Val Leu Cys Leu Tyr Ala Ser Val

530 535 540

Thr Val Val Glu Ser Leu Met Met Ala Ile Ala Ser Val Ile Pro Asn

545 550 555 560

Phe Leu Met Gly Ile Ile Ile Gly Ala Gly Ile Gln Gly Ile Phe Met

565 570 575

Leu Val Ser Gly Tyr Phe Arg Leu Pro His Asp Ile Pro Lys Pro Phe

580 585 590

Trp Arg Tyr Pro Met Ser Tyr Ile Ser Phe His Tyr Trp Ala Leu Gln

595 600 605

Gly Gln Tyr Gln Asn Asp Leu Lys Gly Leu Val Phe Asp Asn Gln Asp

610 615 620

Asp Glu Leu Pro Lys Ile Pro Gly Glu Tyr Ile Leu Glu Asn Val Phe

625 630 635 640

Gln Ile Asp Val Ser Arg Ser Lys Trp Leu Asp Leu Ala Val Leu Phe

645 650 655

Ser Met Ile Phe Ile Tyr Arg Leu Leu Phe Phe Ala Met Ile Lys Val

660 665 670

Ser Glu Asp Val Thr Pro Trp Val Arg Gly Tyr Val Ala Arg Arg Arg

675 680 685

Val Gln Gly Lys Gly Ala Arg Gly Arg Gly Ala Asp Leu Ser Ala Ala

690 695 700

Arg Ser Pro Ser Leu Arg Ala Tyr Val Val Asp Ala Ala Asp Asp Leu

705 710 715 720

Pro Pro Ala

           

<210> 7

<211> 2392

<212> DNA

<213> paddy rice (Oryza sativa)

<400> 7

atgaagaagc agggaggagg aggaggagta gcggcggcgg cggtggcggc ggcggggatg 60

gtgaaggcgg agctggagga cgtggggatc aaggcggcgg gaggggcggt ggcggcgctg 120

agcccgctga gcgagacgct gtggcgggag aaggcggcgg cggagttcct cggcgacgtg 180

tcggcgcgcc tggcgtggcg cgacctcacc gtcaccgtcg tgctcggcgg cggcggcggc 240

ggcggcggag gagggacgac gcaggacgtg ctccaggggc tcacggggca cgccgagccg 300

ggcaccatca cggcgctcat gggcccctcc ggctccggca agtccaccct gctcgacgcc 360

ctcgccggcc gcctcgccgc caacgccttc ctcgacggca ccgtcctcct caacggccgc 420

aaggccaacc tctccttcgg cgccgcggcc tatgtgaccc aagatgacaa cttgatcggc 480

acattgacgg tgagggagac catctcatac tcggcgcggc ttcgccttcc cgacaagatg 540

cccatggaag agaagcgcgc ccttgtcgag ggcacaatcg tcgagatggg gctgcaggac 600

tgcgccgaca cggtggtcgg caattggcac ctgaggggaa tcagtggtgg tgagaagagg 660

agggtcagca ttgcccttga gatactgatg aggcccaggc tgctcttcct ggatgaacca 720

accagtggcc ttgacagtgc ttcagcgttc ttcgtgacac agacactgcg tgggctagcg 780

agggatggca ggacagtgat agcatcgatc catcagccga gcagcgaggt ttttgagctt 840

ttcgaccgct tgtatctcct ttcaggaggc aaaactgttt actttgggct ggcctctgaa 900

gcttgtcagt tctttgccca agccggcttc ccttgcccac cgctgcgcaa tccatcggat 960

catttcctca gatgcataaa cgcagacttc gacaaggtga aggccactct gaaaggatca 1020

atgaagagaa gatttgaaag atccgatgac ccacttgatc ggattatgac ttcggaggcc 1080

atcagaaggc tgatcaccta ctacaagaac tcacagtact actttgctgc gcagcagaaa 1140

gtcaatgaga tggcacgggt taaagggaca gtgctggatg caggagggag ccaggctagc 1200

ttttggatgc aggctttcac actcacgaag cgatcgttca tcaacatgtc gcgggacttc 1260

gggtactact ggctgaggct tatcatctac attgttgtga cagtctgcat cgggacaatc 1320

tacctcaatg ttggcaccag atacagctca attctggcaa gaggtgcatg cgcttctttc 1380

gtcttcggct ttgtcacgtt catgtcgatt ggaggattcc catcttttgt ggaagacatg 1440

aaggtgtttc agagagagag gctcaatggg cactatggcg tgctggcatt cgtgataagc 1500

aacacgatct cggcgatgcc attcttgatc ttgatcacct tcatctccgg gacaatgtgc 1560

tacttcatgg tgcgcctcca cccgggcttc acgcactacc tcttcttcgt cctctgcctc 1620

tacgcaagcg tcaccgtcgt cgagagcttg atgatggcca ttgccagtgt catccccaac 1680

ttcctcatgg gcatcatcat cggtgcagga attcagatga attaattacc atccatcaca 1740

acaacttgat cctgtccatc tttatcaggc actgtttgga gtcctgcaac gcatacaaac 1800

tgggattaaa ttttgacact gtacttggaa ctgcacaaca taatgttgat gtttgttaat 1860

attctctcat gatcatgaca tgcaacaaca tttattgaag agaaaaatat cttttggaaa 1920

tggacagggg atattcatgc tggtttcagg atacttcagg cttccccatg acatcccaaa 1980

gccattctgg aggtatccca tgtcatacat cagcttccac tactgggcat tgcagggtca 2040

gtaccagaac gacctgaagg gcctggtgtt cgacaaccag gacgacgagc tccccaagat 2100

ccccggcgag tacatcctgg agaacgtgtt ccagatcgac gtgagccgat ccaagtggct 2160

ggacctcgcc gtgctcttca gcatgatctt catctaccgc ctcctcttct tcgccatgat 2220

caaggtcagc gaggacgtca ccccgtgggt gcgcggctac gtcgcgcggc gccgggtgca 2280

gggcaagggc gcccgcggcg cccgcggccg cggggcggac ctcgccgccg cgcgctcgcc 2340

gtcgctccgc gcctacgtcg tcgacgccgc cgacgacctg ccgccggcct ga 2392

<210> 8

<211> 574

<212> PRT

<213> paddy rice (Oryza sativa)

<400> 8

Met Lys Lys Gln Gly Gly Gly Gly Gly Val Ala Ala Ala Ala Val Ala

1 5 10 15

Ala Ala Gly Met Val Lys Ala Glu Leu Glu Asp Val Gly Ile Lys Ala

20 25 30

Ala Gly Gly Ala Val Ala Ala Leu Ser Pro Leu Ser Glu Thr Leu Trp

35 40 45

Arg Glu Lys Ala Ala Ala Glu Phe Leu Gly Asp Val Ser Ala Arg Leu

50 55 60

Ala Trp Arg Asp Leu Thr Val Thr Val Val Leu Gly Gly Gly Gly Gly

65 70 75 80

Gly Gly Gly Gly Gly Thr Thr Gln Asp Val Leu Gln Gly Leu Thr Gly

85 90 95

His Ala Glu Pro Gly Thr Ile Thr Ala Leu Met Gly Pro Ser Gly Ser

100 105 110

Gly Lys Ser Thr Leu Leu Asp Ala Leu Ala Gly Arg Leu Ala Ala Asn

115 120 125

Ala Phe Leu Asp Gly Thr Val Leu Leu Asn Gly Arg Lys Ala Asn Leu

130 135 140

Ser Phe Gly Ala Ala Ala Tyr Val Thr Gln Asp Asp Asn Leu Ile Gly

145 150 155 160

Thr Leu Thr Val Arg Glu Thr Ile Ser Tyr Ser Ala Arg Leu Arg Leu

165 170 175

Pro Asp Lys Met Pro Met Glu Glu Lys Arg Ala Leu Val Glu Gly Thr

180 185 190

Ile Val Glu Met Gly Leu Gln Asp Cys Ala Asp Thr Val Val Gly Asn

195 200 205

Trp His Leu Arg Gly Ile Ser Gly Gly Glu Lys Arg Arg Val Ser Ile

210 215 220

Ala Leu Glu Ile Leu Met Arg Pro Arg Leu Leu Phe Leu Asp Glu Pro

225 230 235 240

Thr Ser Gly Leu Asp Ser Ala Ser Ala Phe Phe Val Thr Gln Thr Leu

245 250 255

Arg Gly Leu Ala Arg Asp Gly Arg Thr Val Ile Ala Ser Ile His Gln

260 265 270

Pro Ser Ser Glu Val Phe Glu Leu Phe Asp Arg Leu Tyr Leu Leu Ser

275 280 285

Gly Gly Lys Thr Val Tyr Phe Gly Leu Ala Ser Glu Ala Cys Gln Phe

290 295 300

Phe Ala Gln Ala Gly Phe Pro Cys Pro Pro Leu Arg Asn Pro Ser Asp

305 310 315 320

His Phe Leu Arg Cys Ile Asn Ala Asp Phe Asp Lys Val Lys Ala Thr

325 330 335

Leu Lys Gly Ser Met Lys Arg Arg Phe Glu Arg Ser Asp Asp Pro Leu

340 345 350

Asp Arg Ile Met Thr Ser Glu Ala Ile Arg Arg Leu Ile Thr Tyr Tyr

355 360 365

Lys Asn Ser Gln Tyr Tyr Phe Ala Ala Gln Gln Lys Val Asn Glu Met

370 375 380

Ala Arg Val Lys Gly Thr Val Leu Asp Ala Gly Gly Ser Gln Ala Ser

385 390 395 400

Phe Trp Met Gln Ala Phe Thr Leu Thr Lys Arg Ser Phe Ile Asn Met

405 410 415

Ser Arg Asp Phe Gly Tyr Tyr Trp Leu Arg Leu Ile Ile Tyr Ile Val

420 425 430

Val Thr Val Cys Ile Gly Thr Ile Tyr Leu Asn Val Gly Thr Arg Tyr

435 440 445

Ser Ser Ile Leu Ala Arg Gly Ala Cys Ala Ser Phe Val Phe Gly Phe

450 455 460

Val Thr Phe Met Ser Ile Gly Gly Phe Pro Ser Phe Val Glu Asp Met

465 470 475 480

Lys Val Phe Gln Arg Glu Arg Leu Asn Gly His Tyr Gly Val Leu Ala

485 490 495

Phe Val Ile Ser Asn Thr Ile Ser Ala Met Pro Phe Leu Ile Leu Ile

500 505 510

Thr Phe Ile Ser Gly Thr Met Cys Tyr Phe Met Val Arg Leu His Pro

515 520 525

Gly Phe Thr His Tyr Leu Phe Phe Val Leu Cys Leu Tyr Ala Ser Val

530 535 540

Thr Val Val Glu Ser Leu Met Met Ala Ile Ala Ser Val Ile Pro Asn

545 550 555 560

Phe Leu Met Gly Ile Ile Ile Gly Ala Gly Ile Gln Met Asn

565 570

<210> 9

<211> 950

<212> DNA

<213> paddy rice (Oryza sativa)

<400> 9

accatatggc tgttcacaac ccaacaactt gcaatccagt gcatgcatta catggccaag 60

aatccagcgg ttgagccact gacatgtata ttccactttc gttatgaccc gcatgtcagt 120

ggctcaattg cacatacagc taagtcagag aatttccttc atctctacca atctattctc 180

tctctttggc ctggtttagt cccaaaacat catatcgaat ctttgaagac atgcatgtag 240

cattaaatat ataataaaaa aaactaattg catagttagg cggaaatcac gagacgaatc 300

ttttgagcct aataagtcca ttattagccg taagtgctac agtaacccac atacgctaat 360

gacggattaa ttaagctcaa aagattcgtc ttgcgatttt caggcaagtt atgaaattag 420

ttttttcatt cgtgtccgaa aacccctacc gatatccagt caaacatccg atgtgacacc 480

caaacatttc ttttcttgaa ttaaacatgc cctttctctt gtgcttgcat tgcatatgca 540

tatgcacaat gcattgcttc tagatcacac caaacagcta ctagcccccc cttatgacat 600

gccattcatc tccattgcat gcttgcatat tgcatgcatg ctgctgctgc ttctactcct 660

ccaactctct agagctagct accttgaccc taggtcaaac ccctacaccc ctcattactt 720

ctgcacctga tctccttctc tctctagctt ctctccctag attttctctc tctaacctgc 780

tcctccatta ctggccttta tatccaccac ctcctcctct tctcctccct cataggtggt 840

gtttgcttag ctagctagct agctccatca ctagctctcg catcgcatgc cacggtgagc 900

cggtgtcgcc ggcgtcgtac ggacgtagca agcgagagag ggagagatga 950

<210> 10

<211> 2631

<212> DNA

<213> barley (Hordeum vulgare)

<400> 10

agagagagag agagcgcaca gagcagccta tagttgcagc ttggacacgc tgcccaagaa 60

acgccattct tctcccgcgc cggcctggct tgtacggtca gctagcttcc tcccacacgc 120

agccgcggcg aggaggaagg cgccgtgtgc gagcgttccc accaccttgt taccaagaag 180

aagccccggc ttgccctcgt ctgccctgct ctgtcacggc ctcccaaggt taggtcgtgg 240

tgtctggccg gcgaggctat gaagaaggga ggggcgggga cggcgaagga tgcggcggcg 300

gtggggcagg tgaaggccga gctggaggac gtggggaagg cggcggggcg ggcggtggcg 360

gtggcggcgg cgccggcgct gagcccgctg agcgagacgc tgtggcggga gaagacggcg 420

gtggagttcc tcggggacgt gtcagcgcgg ctggcctgga gggacctcac ggtcaccgtc 480

gcgctcggca gcggcgacac gcagaccgtg ctcgaggggc tcacggggta cgccgagccg 540

ggcaccatca ccgcgctcat gggcccctcc ggctccggca agtccacgct gctcgacgcc 600

ctcgccggcc gcctcgccgc caacgccttc ctctccggga ccatcctgct caacggacgc 660

aaggccaatc tttccttcgg cgccgcggcc tatgtgacgc aagacgacaa cttgattggc 720

actttgacgg tgagggaaac gatctcgtac tcggcacgcc ttcgactccc cgacaacatg 780

cccatggaag agaagcgtgc cttggtggag ggcaccatcg tggagatggg gcttcaggac 840

tgcgcagaca cggtcgtcgg caattggcac ctgagggggg tcagcggcgg cgagaagagg 900

agggtcagca ttgccctgga gatactgatg aggcctaggc tgctcttcct ggatgagccc 960

accagtggcc tcgacagtgc ttcggcgttc ttcgtgacac aaactctgcg tgggctggcc 1020

agagatggcc ggactgtgat cgcgtcgatc caccagccca gcagcgaggt cttcgagctt 1080

ttcgatcgcc tctatcttct ctcagggggc aaaacagtct actttgggca ggcttccgag 1140

gcttgcgagt tctttgccca agctggcttc ccgtgcccgc cgctgcgcaa cccgtcggat 1200

catttcctcc ggtgcataaa cgcggacttt gacaaggtga aggccacact gaaaggatca 1260

atgaagataa ggtttgagag gtccgacgat cccctcgagc acaccactac ttcggacgcg 1320

atcagaaggc tgttcagcta ctaccagcac tcgcagcact acttgacggc actgcagaag 1380

gtcgacgaga tggcacgggt caaaggaacg gtcctggacg caggggggag ccaggccagc 1440

ttcgggatgc aggccttcac gcttaccaag cgatcgttcg tcaacatgtc gagggacttt 1500

ggatactact ggctgaggct tgtcatctac attttggtca cggtctgcat tgggtccatc 1560

tacctcaacg tcggcaccaa atacagctcc atcctggcaa ggggcgcgtg cgcgtccttc 1620

atcttcggct tcgtcacgtt catgtcgatc ggagggttcc cgtctttcgt ggaggacatg 1680

aaggtgttcc agagggagcg gctgaacggg cactacggcg tgctggcgtt cgtgatgagc 1740

aacacgctgt cagcgatgcc gttcctgatc ctcatcacgt tcctgtcggg gacgctgtgc 1800

tacttcatgg tgcgcctcca cccgggcttc acgcactacc tcttcttcct cctctgcctt 1860

tacgccagcg tcaccgtcgt cgagagcctt atgatggcca tcgccagcat catccccaac 1920

ttcctaatgg gcatcatcat cggcgcaggg atacagggga tattcatgct ggtgtcgggg 1980

tacttcaggc tcccacatga catcccgaag cccttctgga ggtaccccat gtcctacatc 2040

agcttccact actgggcact gcaggggcag taccagaacg acctggtggg gctggtgttc 2100

gacaaccagg acgacgagct gcccaagatc ccgggggagt acatcctgga gaacgtgttc 2160

cagatcgacg tgagccggtc caagtggctg gacctgtcgg tgctcttcgg catgatcgtc 2220

atctaccgcc tgctcttctt tgccatgatc aaggtcagcg aggacgtgac gccgtgggtg 2280

cgcggctacg tcgccaggag gagggtgcag cacaagcgca gagaggcgga gctcgccatg 2340

gtccggacgc cgtcgctgcg cggctacgtc atcgacgcgg cgccggagct gccggccgat 2400

catccgtgat ccatcatcga gaatggatga tgaatggcct gagctgaatc ggtatacatg 2460

agccgtgatg tgtgatcctt gtgtggtgtg actaccatct gacgatgcat cattgaaatg 2520

gcatcttgta gcagtaaggt tgtcactcaa atagtgctcc gaatggctac aacttttatc 2580

aagtgtagta ctacaatctg atggttcatc attaaggtgg catcttgtag c 2631

<210> 11

<211> 2151

<212> DNA

<213> barley (Hordeum vulgare)

<400> 11

atgaagaagg gaggggcggg gacggcgaag gatgcggcgg cggtggggca ggtgaaggcc 60

gagctggagg acgtggggaa ggcggcgggg cgggcggtgg cggtggcggc ggcgccggcg 120

ctgagcccgc tgagcgagac gctgtggcgg gagaagacgg cggtggagtt cctcggggac 180

gtgtcagcgc ggctggcctg gagggacctc acggtcaccg tcgcgctcgg cagcggcgac 240

acgcagaccg tgctcgaggg gctcacgggg tacgccgagc cgggcaccat caccgcgctc 300

atgggcccct ccggctccgg caagtccacg ctgctcgacg ccctcgccgg ccgcctcgcc 360

gccaacgcct tcctctccgg gaccatcctg ctcaacggac gcaaggccaa tctttccttc 420

ggcgccgcgg cctatgtgac gcaagacgac aacttgattg gcactttgac ggtgagggaa 480

acgatctcgt actcggcacg ccttcgactc cccgacaaca tgcccatgga agagaagcgt 540

gccttggtgg agggcaccat cgtggagatg gggcttcagg actgcgcaga cacggtcgtc 600

ggcaattggc acctgagggg ggtcagcggc ggcgagaaga ggagggtcag cattgccctg 660

gagatactga tgaggcctag gctgctcttc ctggatgagc ccaccagtgg cctcgacagt 720

gcttcggcgt tcttcgtgac acaaactctg cgtgggctgg ccagagatgg ccggactgtg 780

atcgcgtcga tccaccagcc cagcagcgag gtcttcgagc ttttcgatcg cctctatctt 840

ctctcagggg gcaaaacagt ctactttggg caggcttccg aggcttgcga gttctttgcc 900

caagctggct tcccgtgccc gccgctgcgc aacccgtcgg atcatttcct ccggtgcata 960

aacgcggact ttgacaaggt gaaggccaca ctgaaaggat caatgaagat aaggtttgag 1020

aggtccgacg atcccctcga gcacaccact acttcggacg cgatcagaag gctgttcagc 1080

tactaccagc actcgcagca ctacttgacg gcactgcaga aggtcgacga gatggcacgg 1140

gtcaaaggaa cggtcctgga cgcagggggg agccaggcca gcttcgggat gcaggccttc 1200

acgcttacca agcgatcgtt cgtcaacatg tcgagggact ttggatacta ctggctgagg 1260

cttgtcatct acattttggt cacggtctgc attgggtcca tctacctcaa cgtcggcacc 1320

aaatacagct ccatcctggc aaggggcgcg tgcgcgtcct tcatcttcgg cttcgtcacg 1380

ttcatgtcga tcggagggtt cccgtctttc gtggaggaca tgaaggtgtt ccagagggag 1440

cggctgaacg ggcactacgg cgtgctggcg ttcgtgatga gcaacacgct gtcagcgatg 1500

ccgttcctga tcctcatcac gttcctgtcg gggacgctgt gctacttcat ggtgcgcctc 1560

cacccgggct tcacgcacta cctcttcttc ctcctctgcc tttacgccag cgtcaccgtc 1620

gtcgagagcc ttatgatggc catcgccagc atcatcccca acttcctaat gggcatcatc 1680

atcggcgcag ggatacaggg gatattcatg ctggtgtcgg ggtacttcag gctcccacat 1740

gacatcccga agcccttctg gaggtacccc atgtcctaca tcagcttcca ctactgggca 1800

ctgcaggggc agtaccagaa cgacctggtg gggctggtgt tcgacaacca ggacgacgag 1860

ctgcccaaga tcccggggga gtacatcctg gagaacgtgt tccagatcga cgtgagccgg 1920

tccaagtggc tggacctgtc ggtgctcttc ggcatgatcg tcatctaccg cctgctcttc 1980

tttgccatga tcaaggtcag cgaggacgtg acgccgtggg tgcgcggcta cgtcgccagg 2040

aggagggtgc agcacaagcg cagagaggcg gagctcgcca tggtccggac gccgtcgctg 2100

cgcggctacg tcatcgacgc ggcgccggag ctgccggccg atcatccgtg a 2151

<210> 12

<211> 716

<212> PRT

<213> barley (Hordeum vulgare)

<400> 12

Met Lys Lys Gly Gly Ala Gly Thr Ala Lys Asp Ala Ala Ala Val Gly

1 5 10 15

Gln Val Lys Ala Glu Leu Glu Asp Val Gly Lys Ala Ala Gly Arg Ala

20 25 30

Val Ala Val Ala Ala Ala Pro Ala Leu Ser Pro Leu Ser Glu Thr Leu

35 40 45

Trp Arg Glu Lys Thr Ala Val Glu Phe Leu Gly Asp Val Ser Ala Arg

50 55 60

Leu Ala Trp Arg Asp Leu Thr Val Thr Val Ala Leu Gly Ser Gly Asp

65 70 75 80

Thr Gln Thr Val Leu Glu Gly Leu Thr Gly Tyr Ala Glu Pro Gly Thr

85 90 95

Ile Thr Ala Leu Met Gly Pro Ser Gly Ser Gly Lys Ser Thr Leu Leu

100 105 110

Asp Ala Leu Ala Gly Arg Leu Ala Ala Asn Ala Phe Leu Ser Gly Thr

115 120 125

Ile Leu Leu Asn Gly Arg Lys Ala Asn Leu Ser Phe Gly Ala Ala Ala

130 135 140

Tyr Val Thr Gln Asp Asp Asn Leu Ile Gly Thr Leu Thr Val Arg Glu

145 150 155 160

Thr Ile Ser Tyr Ser Ala Arg Leu Arg Leu Pro Asp Asn Met Pro Met

165 170 175

Glu Glu Lys Arg Ala Leu Val Glu Gly Thr Ile Val Glu Met Gly Leu

180 185 190

Gln Asp Cys Ala Asp Thr Val Val Gly Asn Trp His Leu Arg Gly Val

195 200 205

Ser Gly Gly Glu Lys Arg Arg Val Ser Ile Ala Leu Glu Ile Leu Met

210 215 220

Arg Pro Arg Leu Leu Phe Leu Asp Glu Pro Thr Ser Gly Leu Asp Ser

225 230 235 240

Ala Ser Ala Phe Phe Val Thr Gln Thr Leu Arg Gly Leu Ala Arg Asp

245 250 255

Gly Arg Thr Val Ile Ala Ser Ile His Gln Pro Ser Ser Glu Val Phe

260 265 270

Glu Leu Phe Asp Arg Leu Tyr Leu Leu Ser Gly Gly Lys Thr Val Tyr

275 280 285

Phe Gly Gln Ala Ser Glu Ala Cys Glu Phe Phe Ala Gln Ala Gly Phe

290 295 300

Pro Cys Pro Pro Leu Arg Asn Pro Ser Asp His Phe Leu Arg Cys Ile

305 310 315 320

Asn Ala Asp Phe Asp Lys Val Lys Ala Thr Leu Lys Gly Ser Met Lys

325 330 335

Ile Arg Phe Glu Arg Ser Asp Asp Pro Leu Glu His Thr Thr Thr Ser

340 345 350

Asp Ala Ile Arg Arg Leu Phe Ser Tyr Tyr Gln His Ser Gln His Tyr

355 360 365

Leu Thr Ala Leu Gln Lys Val Asp Glu Met Ala Arg Val Lys Gly Thr

370 375 380

Val Leu Asp Ala Gly Gly Ser Gln Ala Ser Phe Gly Met Gln Ala Phe

385 390 395 400

Thr Leu Thr Lys Arg Ser Phe Val Asn Met Ser Arg Asp Phe Gly Tyr

405 410 415

Tyr Trp Leu Arg Leu Val Ile Tyr Ile Leu Val Thr Val Cys Ile Gly

420 425 430

Ser Ile Tyr Leu Asn Val Gly Thr Lys Tyr Ser Ser Ile Leu Ala Arg

435 440 445

Gly Ala Cys Ala Ser Phe Ile Phe Gly Phe Val Thr Phe Met Ser Ile

450 455 460

Gly Gly Phe Pro Ser Phe Val Glu Asp Met Lys Val Phe Gln Arg Glu

465 470 475 480

Arg Leu Asn Gly His Tyr Gly Val Leu Ala Phe Val Met Ser Asn Thr

485 490 495

Leu Ser Ala Met Pro Phe Leu Ile Leu Ile Thr Phe Leu Ser Gly Thr

500 505 510

Leu Cys Tyr Phe Met Val Arg Leu His Pro Gly Phe Thr His Tyr Leu

515 520 525

Phe Phe Leu Leu Cys Leu Tyr Ala Ser Val Thr Val Val Glu Ser Leu

530 535 540

Met Met Ala Ile Ala Ser Ile Ile Pro Asn Phe Leu Met Gly Ile Ile

545 550 555 560

Ile Gly Ala Gly Ile Gln Gly Ile Phe Met Leu Val Ser Gly Tyr Phe

565 570 575

Arg Leu Pro His Asp Ile Pro Lys Pro Phe Trp Arg Tyr Pro Met Ser

580 585 590

Tyr Ile Ser Phe His Tyr Trp Ala Leu Gln Gly Gln Tyr Gln Asn Asp

595 600 605

Leu Val Gly Leu Val Phe Asp Asn Gln Asp Asp Glu Leu Pro Lys Ile

610 615 620

Pro Gly Glu Tyr Ile Leu Glu Asn Val Phe Gln Ile Asp Val Ser Arg

625 630 635 640

Ser Lys Trp Leu Asp Leu Ser Val Leu Phe Gly Met Ile Val Ile Tyr

645 650 655

Arg Leu Leu Phe Phe Ala Met Ile Lys Val Ser Glu Asp Val Thr Pro

660 665 670

Trp Val Arg Gly Tyr Val Ala Arg Arg Arg Val Gln His Lys Arg Arg

675 680 685

Glu Ala Glu Leu Ala Met Val Arg Thr Pro Ser Leu Arg Gly Tyr Val

690 695 700

Ile Asp Ala Ala Pro Glu Leu Pro Ala Asp His Pro

705 710 715

<210> 13

<211> 5321

<212> DNA

<213> Chinese sorghum (Sorghum bicolor)

<400> 13

accgcatcga ccggccggtt cctctctccc gacacgacgc gccccagccc agtgcacggc 60

cccgccacgc tagcctccca gcagctccca gccccagctc cgctcagggc gcgcctgcag 120

ttccggccat gagacgggcc ggaggaggag acctgcaggg cgaccacggc acgacgcctc 180

ggtccgcggc ggcagggcag gccatggtgg agctgcaggc caacgggtcg gcggccgccg 240

ccgggggcgc catggtggtg ggtctcagcc cgctcagcga gaccctgtgg cgggacagca 300

aggcgctccc gccgggtgcc ggccccgccg cgctcatcgg cgacgtgtcc gccaggctca 360

cgtggaagga cctctgcgtc accgtggcgc tcggccccgg caagacgcag accgtgctgg 420

acgaactcac cgggtacgcg gagcccggct cgctcaccgc gctcatgggc ccctccgggt 480

ccggcaagtc caccctgctc gacgccctcg ccggccgcct cgccgccaac gccttcctct 540

ccggcgacgt gctcctcaac ggccgcaagg ccaagctctc cttcggcgcc gcggtacgtg 600

acctttgctg cttcctcagt cctcgcttaa gctcctagct atagatcatg cagcactact 660

ctctctcaac tgggaaaaaa tagaatcact gttctatttt tgagcaccaa aaacactccg 720

tactgctact acttcagcca gatcatccat atataggagc tagcctagca gttccgttaa 780

gggtttctta ggtaggatct gttcaagttc gaaactatct tccatatata actggcatct 840

cgcaacatgc aaatcatatt tgaaggtatc cggatgaccg gatctggtaa tctacttggt 900

gtgtcagtaa aacaatcgtt gctggtaatt aaagtgtggt ttgtctatgg tttttccaaa 960

tgaaatatac tatatatatg ctgcgtcctg ttattatcac tataatcaat gacataacta 1020

gtactctcat tctgttcttt tttaaaagac ctaccacaac acagctacgc tttgaccact 1080

atagtagtgc tataattaag cagaggtgcg agctgcttat ttcatgtcgg gcagtaagca 1140

gggagatcgg ctagccggca tagtgttatt agcgacagat cacagataca cgtccaaatc 1200

caatcgattc gggtcgattt ttggcgtccg gccaagatgt cgaaagcacg tacggctagg 1260

ctttgcatag tagagctccg gcgtgtaatg ttgttaggtg cgcggattta attcgccctc 1320

gatcggttgg tcgatggcgc ctccactgga attcattcaa acacataggt cgtactgcgt 1380

actgctgcta gtatgattag tatcgtgatt ttgatctctc acatcgatag atccagaggc 1440

gaaaaacatg gcgatcgata tcgtcctgct ctgctgtcaa tacaatacac atggcgatcg 1500

atatccacaa cggctgaagc cacctacaca gcataaatac tgacagctgg gtttttctct 1560

ctgatcctgc tgcgaattta tggtgatacg aaccgtccgg gctgtggcgt ttgggtacgt 1620

ctcaccagca gttacacgca acgggatcct gcaggcgcat caaacgcatc agttttctgg 1680

ctaatcttgc tctgatccag agtagaaagt tttaagtgga cgagtttttt tttttatggg 1740

agagtttgtg ctactactgc cggaggagga gactgatcca gaggcgttgt tggtgccgtg 1800

tgtatacgcg cgcgtacgtg cttcttctgc ttgcaggcgt acgtgacgca ggacgacaac 1860

ctgatcggga cgctgacggt gcgcgagacg atcggctact cggcgctgct gcggctgccg 1920

gacaagatgc cgcgggagga caagcgcgcg ctggtggagg gcaccatcgt cgagatgggg 1980

ctccaggact gcgccgacac cgtcatcggg aactggcacc tccgcggggt cagcggcggc 2040

gagaagcgcc gcgtcagcat cgcgctcgag ctcctcatgc gcccgcgcct cctcttcctc 2100

gacgagccca ccagcggcct cgacaggtga gcagcacggt ggcggccgtg gtgcacggcg 2160

gcaaattgtg tgagcctgca atgtcagttg gtggtggtga ggctaactta tttagctgcg 2220

ggcgtggctg tatgtgtgca gctcctcggc gttcttcgtg acgcagacgc tgcgaggcct 2280

ggccagggac ggcaggacag tgattgcttc catccaccag cccagcagcg aggtgttcga 2340

gctcttcgac atgctcttcc tgctgtccgg gggcaaaacc gtctacttcg gccaagcatc 2400

gcaagcatgt gaggtagtaa tcctgtattc ttgccggtgt taccgtgaga tgccgaatgc 2460

atgcttcggc gacaatgtga tcgtgcgact gactctcctt gcattgctcg gttgcagttc 2520

tttgctcaag gcggtttccc ttgcccgcct ctgaggaacc catcggacca tttcctgagg 2580

tgcgtcaact cggacttcga caaggtgaag gccacactga aaggatcaat gaaggcaagg 2640

gtaagaagaa gaaggtccag gggcaaatca gaggccacca aaacatgaaa aaaaaagcct 2700

tgttctctgc tgtgaataaa atactaatac gaccttttat tttgtgttca tgaaaaggct 2760

gagaggtccg acgatccact cgataggatg actacatcag aagccatcag gaaattggtt 2820

ggatcctaca gcaggtccca atactactac gctgcgaggg agaaagttaa tgatatctcg 2880

agaatggtac gtattctgag tcagcaaaca caacattatg cattgatatg ttgtatctgt 2940

ttcctgtgtc cacataataa ataccgcatc tggggctggg aaaaactaac cataaccatc 3000

ttgttcctta cagaaaggaa ctgtgctgga ttcaggtggc agccaggcta gcttcctgat 3060

gcaggcttgc accctgacca agcgctcgtt catcaacatg tcacgggact ttgggtatta 3120

ctggctcagg ctcctgatct atctcctcgt gactgtttgc atcggcacaa tctacctgga 3180

tgttggcact aaatacacat ccatcctggt aagtaggaag gaaagaaatt ggtccggaag 3240

ttctgggctg gactgatatg aatgcacaat aatgtagtca catgaccaat gtcatgaagc 3300

agcatatggt tgattttaat gcattcactc taccttgcct ctgcaggcca gagcttcctg 3360

ttctgcattc gtctttgggt tcgttacgtt catgtccatt ggaggattcc cttcgtttgt 3420

tgaagaaatg aaggtgaaat taaaatggtg ttttttcatc tgtaaattag aaatgctatg 3480

aattttgctc acctttcgtt tgcttcattt atacaggtct tccaacggga acggctgaat 3540

gggcactatg gtgtcgcggc atttgtcatc agtaacacca tctcagcaac gccattcctg 3600

attctaatat gcttcttgtc gggaactata tgctacttta tggtgcgcct gcatcctggt 3660

ttcgaacact atatcttctt cgtcttgaac ctgtatgcca gcgtcacagt ggtcgaaagc 3720

ttaatgatgg caattgccag tgtcattccc aatttcctca tgggtatcat cataggggct 3780

ggaattcagg tatgacattt tcacccatct cccatatcgg tttatcatca gcccttataa 3840

cctattatag cttgggggat atgtgttcaa aatatatggt taagtatggg gttatgaatc 3900

aacttgtaaa atatcatttt agctcagatg ttgagactgc acttttagtt gtggcataag 3960

ttgacacata caaccatgtg atacttaaga tgaattcatg aactaaagaa cctccatact 4020

tgaggtggtt ttccgggacc agaatatcaa aggactttat cctctattga tccaatatcc 4080

agcttgtcct gacctgtatt atttggagct tcactacatg ctggccaaca ttctagtaca 4140

agctcagcaa attttaggac acgagacatt ggtgaacttg tagctatcac tggattttac 4200

tttattttat tggatttgct tagctattgg tgcagaatac acattattcg tgatttaatg 4260

tttatttagt tatgttcagc agttccatgc caccgtaaat agacatcacc atactcccct 4320

cgaaaagatt atgagtttcg aatttatcat gatgaaatat tctcaacaat tttcctgtac 4380

agggaatatt tatgctggtg tctggctact tcagacttcc atacgacatt ccaaagcctt 4440

tttggagata ccccatgcag tacatcagct tccattactg ggcactgcag gtaaattccg 4500

tttcacacat tggagcaacc cacatacact agcctccaag tctccaacct gaactttttg 4560

acaccaaact ccatacatct aacagggcca gtgccagaac gacatgaaag gcctcgtatt 4620

cgacaaccag tacccagatc aacccaagat ccccggggac ttcatcctga agtacatctt 4680

ccagatcaac gtggaccgga acaagtggat cgacttgtca gtcattttca gcatgatctt 4740

catctaccgc atcctcttct tcctcatgat caagatcaac gaggatgtgc tgccgtggat 4800

ccgtggccac atcgcgagga agagaatgca gaagaaaggc cctagtccca gtttcggcaa 4860

gacgccttcg ctgagaggct atgttgtgga tccagagctc ggttccaacg agggctagag 4920

gacgatgatg atctggttct tttgggtagc agaaataggg tgtttctccc cccggctctt 4980

ggtgctatat tgtatattta agatcaggga aaattaaact ggtatcatac gttttgaaac 5040

tgaagctaat tcgagtacag gtatacatac agcatgtaat gtgactatgt gagcaaagtg 5100

gcatcggaga gatgcccagt gacacatgac agattgttca gaatttattg ccttcgacac 5160

acgaagaatt gttggagcac taaagcgggt acggctcaca tgaccgtgta ttatcagatt 5220

gattttgatt gcttgctggt tacataaaat cggtactaca tattgacaga attgtaaatg 5280

ccccacataa atataaacta acaataagat ccatgccgta c 5321

<210> 14

<211> 2175

<212> DNA

<213> Chinese sorghum (Sorghum bicolor)

<400> 14

atgagacggg ccggaggagg agacctgcag ggcgaccacg gcacgacgcc tcggtccgcg 60

gcggcagggc aggccatggt ggagctgcag gccaacgggt cggcggccgc cgccgggggc 120

gccatggtgg tgggtctcag cccgctcagc gagaccctgt ggcgggacag caaggcgctc 180

ccgccgggtg ccggccccgc cgcgctcatc ggcgacgtgt ccgccaggct cacgtggaag 240

gacctctgcg tcaccgtggc gctcggcccc ggcaagacgc agaccgtgct ggacgaactc 300

accgggtacg cggagcccgg ctcgctcacc gcgctcatgg gcccctccgg gtccggcaag 360

tccaccctgc tcgacgccct cgccggccgc ctcgccgcca acgccttcct ctccggcgac 420

gtgctcctca acggccgcaa ggccaagctc tccttcggcg ccgcggcgta cgtgacgcag 480

gacgacaacc tgatcgggac gctgacggtg cgcgagacga tcggctactc ggcgctgctg 540

cggctgccgg acaagatgcc gcgggaggac aagcgcgcgc tggtggaggg caccatcgtc 600

gagatggggc tccaggactg cgccgacacc gtcatcggga actggcacct ccgcggggtc 660

agcggcggcg agaagcgccg cgtcagcatc gcgctcgagc tcctcatgcg cccgcgcctc 720

ctcttcctcg acgagcccac cagcggcctc gacagctcct cggcgttctt cgtgacgcag 780

acgctgcgag gcctggccag ggacggcagg acagtgattg cttccatcca ccagcccagc 840

agcgaggtgt tcgagctctt cgacatgctc ttcctgctgt ccgggggcaa aaccgtctac 900

ttcggccaag catcgcaagc atgtgagttc tttgctcaag gcggtttccc ttgcccgcct 960

ctgaggaacc catcggacca tttcctgagg tgcgtcaact cggacttcga caaggtgaag 1020

gccacactga aaggatcaat gaaggcaagg gctgagaggt ccgacgatcc actcgatagg 1080

atgactacat cagaagccat caggaaattg gttggatcct acagcaggtc ccaatactac 1140

tacgctgcga gggagaaagt taatgatatc tcgagaatga aaggaactgt gctggattca 1200

ggtggcagcc aggctagctt cctgatgcag gcttgcaccc tgaccaagcg ctcgttcatc 1260

aacatgtcac gggactttgg gtattactgg ctcaggctcc tgatctatct cctcgtgact 1320

gtttgcatcg gcacaatcta cctggatgtt ggcactaaat acacatccat cctggccaga 1380

gcttcctgtt ctgcattcgt ctttgggttc gttacgttca tgtccattgg aggattccct 1440

tcgtttgttg aagaaatgaa ggtcttccaa cgggaacggc tgaatgggca ctatggtgtc 1500

gcggcatttg tcatcagtaa caccatctca gcaacgccat tcctgattct aatatgcttc 1560

ttgtcgggaa ctatatgcta ctttatggtg cgcctgcatc ctggtttcga acactatatc 1620

ttcttcgtct tgaacctgta tgccagcgtc acagtggtcg aaagcttaat gatggcaatt 1680

gccagtgtca ttcccaattt cctcatgggt atcatcatag gggctggaat tcagggaata 1740

tttatgctgg tgtctggcta cttcagactt ccatacgaca ttccaaagcc tttttggaga 1800

taccccatgc agtacatcag cttccattac tgggcactgc agggccagtg ccagaacgac 1860

atgaaaggcc tcgtattcga caaccagtac ccagatcaac ccaagatccc cggggacttc 1920

atcctgaagt acatcttcca gatcaacgtg gaccggaaca agtggatcga cttgtcagtc 1980

attttcagca tgatcttcat ctaccgcatc ctcttcttcc tcatgatcaa gatcaacgag 2040

gatgtgctgc cgtggatccg tggccacatc gcgaggaaga gaatgcagaa gaaaggccct 2100

agtcccagtt tcggcaagac gccttcgctg agaggctatg ttgtggatcc agagctcggt 2160

tccaacgagg gctag 2175

<210> 15

<211> 724

<212> PRT

<213> Chinese sorghum (Sorghum bicolor)

<400> 15

Met Arg Arg Ala Gly Gly Gly Asp Leu Gln Gly Asp His Gly Thr Thr

1 5 10 15

Pro Arg Ser Ala Ala Ala Gly Gln Ala Met Val Glu Leu Gln Ala Asn

20 25 30

Gly Ser Ala Ala Ala Ala Gly Gly Ala Met Val Val Gly Leu Ser Pro

35 40 45

Leu Ser Glu Thr Leu Trp Arg Asp Ser Lys Ala Leu Pro Pro Gly Ala

50 55 60

Gly Pro Ala Ala Leu Ile Gly Asp Val Ser Ala Arg Leu Thr Trp Lys

65 70 75 80

Asp Leu Cys Val Thr Val Ala Leu Gly Pro Gly Lys Thr Gln Thr Val

85 90 95

Leu Asp Glu Leu Thr Gly Tyr Ala Glu Pro Gly Ser Leu Thr Ala Leu

100 105 110

Met Gly Pro Ser Gly Ser Gly Lys Ser Thr Leu Leu Asp Ala Leu Ala

115 120 125

Gly Arg Leu Ala Ala Asn Ala Phe Leu Ser Gly Asp Val Leu Leu Asn

130 135 140

Gly Arg Lys Ala Lys Leu Ser Phe Gly Ala Ala Ala Tyr Val Thr Gln

145 150 155 160

Asp Asp Asn Leu Ile Gly Thr Leu Thr Val Arg Glu Thr Ile Gly Tyr

165 170 175

Ser Ala Leu Leu Arg Leu Pro Asp Lys Met Pro Arg Glu Asp Lys Arg

180 185 190

Ala Leu Val Glu Gly Thr Ile Val Glu Met Gly Leu Gln Asp Cys Ala

195 200 205

Asp Thr Val Ile Gly Asn Trp His Leu Arg Gly Val Ser Gly Gly Glu

210 215 220

Lys Arg Arg Val Ser Ile Ala Leu Glu Leu Leu Met Arg Pro Arg Leu

225 230 235 240

Leu Phe Leu Asp Glu Pro Thr Ser Gly Leu Asp Ser Ser Ser Ala Phe

245 250 255

Phe Val Thr Gln Thr Leu Arg Gly Leu Ala Arg Asp Gly Arg Thr Val

260 265 270

Ile Ala Ser Ile His Gln Pro Ser Ser Glu Val Phe Glu Leu Phe Asp

275 280 285

Met Leu Phe Leu Leu Ser Gly Gly Lys Thr Val Tyr Phe Gly Gln Ala

290 295 300

Ser Gln Ala Cys Glu Phe Phe Ala Gln Gly Gly Phe Pro Cys Pro Pro

305 310 315 320

Leu Arg Asn Pro Ser Asp His Phe Leu Arg Cys Val Asn Ser Asp Phe

325 330 335

Asp Lys Val Lys Ala Thr Leu Lys Gly Ser Met Lys Ala Arg Ala Glu

340 345 350

Arg Ser Asp Asp Pro Leu Asp Arg Met Thr Thr Ser Glu Ala Ile Arg

355 360 365

Lys Leu Val Gly Ser Tyr Ser Arg Ser Gln Tyr Tyr Tyr Ala Ala Arg

370 375 380

Glu Lys Val Asn Asp Ile Ser Arg Met Lys Gly Thr Val Leu Asp Ser

385 390 395 400

Gly Gly Ser Gln Ala Ser Phe Leu Met Gln Ala Cys Thr Leu Thr Lys

405 410 415

Arg Ser Phe Ile Asn Met Ser Arg Asp Phe Gly Tyr Tyr Trp Leu Arg

420 425 430

Leu Leu Ile Tyr Leu Leu Val Thr Val Cys Ile Gly Thr Ile Tyr Leu

435 440 445

Asp Val Gly Thr Lys Tyr Thr Ser Ile Leu Ala Arg Ala Ser Cys Ser

450 455 460

Ala Phe Val Phe Gly Phe Val Thr Phe Met Ser Ile Gly Gly Phe Pro

465 470 475 480

Ser Phe Val Glu Glu Met Lys Val Phe Gln Arg Glu Arg Leu Asn Gly

485 490 495

His Tyr Gly Val Ala Ala Phe Val Ile Ser Asn Thr Ile Ser Ala Thr

500 505 510

Pro Phe Leu Ile Leu Ile Cys Phe Leu Ser Gly Thr Ile Cys Tyr Phe

515 520 525

Met Val Arg Leu His Pro Gly Phe Glu His Tyr Ile Phe Phe Val Leu

530 535 540

Asn Leu Tyr Ala Ser Val Thr Val Val Glu Ser Leu Met Met Ala Ile

545 550 555 560

Ala Ser Val Ile Pro Asn Phe Leu Met Gly Ile Ile Ile Gly Ala Gly

565 570 575

Ile Gln Gly Ile Phe Met Leu Val Ser Gly Tyr Phe Arg Leu Pro Tyr

580 585 590

Asp Ile Pro Lys Pro Phe Trp Arg Tyr Pro Met Gln Tyr Ile Ser Phe

595 600 605

His Tyr Trp Ala Leu Gln Gly Gln Cys Gln Asn Asp Met Lys Gly Leu

610 615 620

Val Phe Asp Asn Gln Tyr Pro Asp Gln Pro Lys Ile Pro Gly Asp Phe

625 630 635 640

Ile Leu Lys Tyr Ile Phe Gln Ile Asn Val Asp Arg Asn Lys Trp Ile

645 650 655

Asp Leu Ser Val Ile Phe Ser Met Ile Phe Ile Tyr Arg Ile Leu Phe

660 665 670

Phe Leu Met Ile Lys Ile Asn Glu Asp Val Leu Pro Trp Ile Arg Gly

675 680 685

His Ile Ala Arg Lys Arg Met Gln Lys Lys Gly Pro Ser Pro Ser Phe

690 695 700

Gly Lys Thr Pro Ser Leu Arg Gly Tyr Val Val Asp Pro Glu Leu Gly

705 710 715 720

Ser Asn Glu Gly

               

<210> 16

<211> 3616

<212> DNA

<213> corn (Zea mays)

<400> 16

gagggagcgc attactctgc agcgcggaca cgcccggcac ggcaagccag ccactcttct 60

cgcgtgaagc ttagccattc ttgcggctgg gcaaggaaac tgctgaagcc actagccggc 120

tgcctgctct acggcctccg aaccgaacga ggtgcatcgt gaggaagcag cagcaggggg 180

gggggggggg ggggggcagt ggccgggcgg gatgacgggg agggaggccg tggcttcggc 240

ggaggcggag ctggatcagg actcggcggc ggcggcggcc gcggcgcccg tgctgctgag 300

cccgctcagc gagacgctgt ggcgcgacag ggcggcggcg gcggcgggcg gggtcctcgt 360

cggggacgtg tcggcgcgcc tggcgtggcg cgacctcacc gtgaccgtcg cgctcggcac 420

cggcgacacg caggccgtgc tgcagggcct cacggggcac gccgagcccg gcaccatcac 480

cgcgctcatg ggcccgtccg gctccggcaa gtccacgctg ctcgacgcgc tcgcgggccg 540

cctcgccgcc aacgccttcc tctccgggac cgtcctcctc aacgggcgca aggccaacct 600

ctccttcggc gccgcggtac gtgcgctcgt cctcgatcgg tcttctcctt tttctcctta 660

cgcatgacag aaagaggtgt gttttgttgt tgtctataag ttgttcgtcg atctgatgaa 720

atagacatga caggacaggc tagctctcca cgcacgtttt tctgtgtgaa actgaaaccg 780

agcatttgtc tgtgactgtc agtctcctcc catgaacatg gagtggattt ggatttgtca 840

atctgctttt cagatggatc gtattgccaa tctcacctca cctggaacct tgttgccaat 900

aatactcagg cctacgtgac gcaagacgac aacctgatcg gcacgctgac ggtgagggag 960

acgatctcgt actcggcgcg actccggctc cccgacaaga tgccgaggga ggagaagcag 1020

gctctggtgg agggcaccat cgtggagatg gggctgcagg actgcgccga caccgtcgtc 1080

ggcaactggc acctgagggg gatcagcggc ggcgagaagc ggagggtcag catcgccctg 1140

gaaatactca tgcggcccag gctgctgttc ctggacgagc ctaccagtgg ccttgacagg 1200

tacttttata tatatgcgct tctgtttctt cgtcctatac tactactagt gatgaccgat 1260

gatgcaaaac aatgtgcagt gcttcggcgt tcttcgtgac gcagacgctg cgagggctgg 1320

caagggacgg ccggaccgtc atagcgtcgg tgcaccagcc gagcagcgag gttttcttgc 1380

tgttcgactg cctctacctt ctgtcagggg gcaggacagt ctacttcggg aaggcatcgg 1440

aggcctgcga ggtgaaggca ctggtctcta ctctctactc tactaccgat gcatatggct 1500

ggcatctgag agagccgtct atctgttgcg tgcacagttc ttcgcccagg ctggcttccc 1560

gtgcccgccg atgcgcaacc cgtcggacca tttcctccgg tgcataaact cggatttcga 1620

caaggtgaag gccactctca aagggtcgat gaaggcgagg gtgagtcgga tttcaccacc 1680

tcgctcctta gttttttttt tggctgttgt agcctatata catagcatct cctttcgctc 1740

gtgtagttcg aaaggtccaa cgacgacgac ccgctggaga agatgacgac gtcggaagcg 1800

atgaggaggc tcaccagcta ctaccagcac tcgcagtgcc acatcaacgc gcagcagaaa 1860

gtggacgaga tggcccggct cgtaagcgcc agctgctcca ttctcttgga cgatcgttca 1920

ccgctttcca acacaactga tgaaaggccg ggggggatcc atgtctgtct gtctgtcctc 1980

gcagaaagga acggtgctgg atccaggagg agggagccag gccagcttcc tgatgcaggc 2040

gttcacgctc acgaagcgct cgttcgtcaa catgtccagg gacttcggct actactggct 2100

caggctcatc atctacatcg tcgtcacgct ttgcatcggg actatatacc tcaacgttgg 2160

gaccggatac agctctatcc tggtaattat taacacgcaa catctatcgt cgtcttttgt 2220

tgggttaatt gggcactgaa tgaataataa tgcgtctact tgcttgttct gaatctgact 2280

gatgaaaact actatactac taggcccggg gcgcgtgtgc ttcgttcatc ttcggcttcg 2340

tcacgttcat gtccatcgga gggtttccgt ctttcgtgga ggacatgaag gtctgtctct 2400

gtcatcggag ggtttcctat gctcagggcc tcaggctcag cttattaatt caattcccgt 2460

ttctttataa ttctccaggt gttccagagg gagaggctga acgggcacta cggcgtgctg 2520

gcgttcgtcg tcagcaacac ggtctcggcg atgccgttcc tggtcctcat caccttcgtg 2580

tcgggcacgc tgtgctactt catggtgcgc ctgcacccgg gcttcacgca ctacctcttc 2640

ttcgtgctgg cgctctacgc cagcgtcacc gtcgtcgaga gcctcatgat ggccatcgcc 2700

agcgtcatcc ccaacttcct catgggcatc ataatcggcg cagggatcca ggtaattaaa 2760

ctgagactga gagactgaga ctgagactga gggttcccta atggtcgtcg tggacacaca 2820

caggggatct tcatgctggt gtcgggttac ttcaggcttc cgcacgacat ccccaagccc 2880

ttctggaggt accccatgtc atacatcagc ttccactact gggcgctgca ggggcagtac 2940

cagaacgacc tcaggggcct gctgttcgac aaccaggacg acgagctgcc caggatcccg 3000

ggggagttca tcctggagac cgtgttccag atcgacgtcg cccgctccaa gtggctggac 3060

ctggccgtgc tcttcagcat gatcgtcatc taccgcctgc tcttcttcct catgatcaag 3120

gccagcgagg acgtcacccc ctggctgcgc cgctacgtcg cgcggcgccg ggtgcggaac 3180

cgtcgccacc gcaagccgga cctgcccgcc cggacgccgt cgctccgcgg gtacgtcgtc 3240

gacgcggcca gcctgccgga cgaccacccg tgacgaaaga gcgcgagaga aaacgtacgg 3300

gcagggatgt catttcatat catatcacac agaggctaaa ccggatcgag ctgataataa 3360

cacactcact cactagtact gcaataatat gatgttaagt tggtatagca accgcttaag 3420

tgcccgttcg gttcttcctg aatgctttac tggattggtt ccggattgga atagttcaca 3480

aatttgtatt agctcgatga gttggaatga ttccggcctt caatctggac taaacgaaca 3540

gagctgtaat ggagtgttgc ttgatgcact gctaagatgt acacgtatat gaagagcatg 3600

tgaccatcag ttctac 3616

<210> 17

<211> 2127

<212> DNA

<213> corn (Zea mays)

<400> 17

atgacgggga gggaggccgt ggcggcggcg gaggcggagc tggatcagga ctcggcggcg 60

gcggcggcgc ccgtgctgct gagcccgctc agcgagacgc tgtggcgcga cagggcggcg 120

gcggcggcgg gcggggtcct cgtcggggac gtgtcggcgc gcctggcgtg gcgcgacctc 180

accgtgaccg tcgcgctcgg ctccggcgac acgcaggccg tgctgcaggg cctcacgggg 240

cacgccgagc ccggcaccat caccgcgctc atgggcccgt ccggctccgg caagtccacg 300

ctgctcgacg cgctcgccgg ccgcctcgcc gccaacgcct tcctctccgg gaccgtcctc 360

ctcaacgggc gcaaggccaa cctctccttc ggcgccgcgg cctacgtgac gcaagacgac 420

aacctgatcg gcacgctgac ggtgagggag acgatctcgt actcggcgcg actccggctc 480

cccgacaaga tgccgaggga ggagaagcag gctctggtgg agggcaccat cgtggagatg 540

gggctgcagg actgcgccga caccgtcgtc ggcaactggc acctgagggg gatcagcggc 600

ggcgagaagc ggagggtcag catcgccctg gaaatactca tgcggcccag gctgctgttc 660

ctggacgagc ctaccagtgg ccttgacagt gcttcggcgt tcttcgtgac gcagacgctg 720

cgagggctgg caagggacgg ccggaccgtc atagcgtcgg tgcaccagcc gagcagcgag 780

gttttcttgc tgttcgactg cctctacctt ctgtcggggg gcaggacagt ctacttcggg 840

aaggcatcgg aggcctgcga gttcttcgcg caggctggct tcccgtgccc gccgatgcgc 900

aacccgtcgg accatttcct ccggtgcata aactcggatt tcgacaaggt gaaggccact 960

ctcaaagggt ccatgaaggc gaagttcgaa aggtccaacg acgacgaccc gctggagaag 1020

atgacgacgt cggaagcgat gaggaggctg accagctact accagcactc gcagtgccac 1080

atcaacgcgc agcagaaagt ggacgagatg gcccggctca aaggaacggt gctggatcca 1140

ggaggaggga gccaggccag cttcctgatg caggcgttca cgctcacgaa gcgctcgttc 1200

gtcaacatgt ccagggactt cggctactac tggctcaggc tcatcatcta catcgtcgtc 1260

acgctttgca tcgggactat atacctcaac gttgggaccg gatacagctc tatcctggcc 1320

cggggcgcgt gtgcttcgtt catcttcggc ttcgtcacgt tcatgtccat cggagggttt 1380

ccgtctttcg tggaggacat gaaggtgttc cagagggaga ggctgaacgg gcactacggc 1440

gtgctggcgt tcgtcgtcag caacacggtc tcggcgatgc cgttcctggt cctcatcacc 1500

ttcgtgtcgg gcacgctgtg ctacttcatg gtgcgcctgc acccgggctt cacgcactac 1560

ctcttcttcg tgctggcgct ctacgccagc gtcaccgtcg tcgagagcct catgatggcc 1620

atcgccagcg tcatccccaa cttcctcatg ggcatcataa tcggcgcagg gatccagggg 1680

atcttcatgc tggtgtcggg ttacttcagg cttccgcacg acatccccaa gcccttctgg 1740

aggtacccca tgtcatacat cagcttccac tactgggcgc tgcaggggca gtaccagaac 1800

gacctcaggg gcctgctgtt cgacaaccag gacgacgagc tgcccaggat cccgggggag 1860

ttcatcctgg agaccgtgtt ccagatcgac gtcgcccgct ccaagtggct ggacctggcc 1920

gtgctcttca gcatgatcgt catctaccgc ctgctcttct tcctcatgat caaggccagc 1980

gaggacgtca ccccctggct gcgccgctac gtcgcgcggc gccgggtgcg gaaccgtcgc 2040

caccgcaagc cggacctgcc cgcccggacg ccgtcgctcc gcgggtacgt cgtcgacgcg 2100

gccagcctgc cggacgacca cccgtga 2127

<210> 18

<211> 708

<212> PRT

<213> corn (Zea mays)

<400> 18

Met Thr Gly Arg Glu Ala Val Ala Ala Ala Glu Ala Glu Leu Asp Gln

1 5 10 15

Asp Ser Ala Ala Ala Ala Ala Pro Val Leu Leu Ser Pro Leu Ser Glu

20 25 30

Thr Leu Trp Arg Asp Arg Ala Ala Ala Ala Ala Gly Gly Val Leu Val

35 40 45

Gly Asp Val Ser Ala Arg Leu Ala Trp Arg Asp Leu Thr Val Thr Val

50 55 60

Ala Leu Gly Ser Gly Asp Thr Gln Ala Val Leu Gln Gly Leu Thr Gly

65 70 75 80

His Ala Glu Pro Gly Thr Ile Thr Ala Leu Met Gly Pro Ser Gly Ser

85 90 95

Gly Lys Ser Thr Leu Leu Asp Ala Leu Ala Gly Arg Leu Ala Ala Asn

100 105 110

Ala Phe Leu Ser Gly Thr Val Leu Leu Asn Gly Arg Lys Ala Asn Leu

115 120 125

Ser Phe Gly Ala Ala Ala Tyr Val Thr Gln Asp Asp Asn Leu Ile Gly

130 135 140

Thr Leu Thr Val Arg Glu Thr Ile Ser Tyr Ser Ala Arg Leu Arg Leu

145 150 155 160

Pro Asp Lys Met Pro Arg Glu Glu Lys Gln Ala Leu Val Glu Gly Thr

165 170 175

Ile Val Glu Met Gly Leu Gln Asp Cys Ala Asp Thr Val Val Gly Asn

180 185 190

Trp His Leu Arg Gly Ile Ser Gly Gly Glu Lys Arg Arg Val Ser Ile

195 200 205

Ala Leu Glu Ile Leu Met Arg Pro Arg Leu Leu Phe Leu Asp Glu Pro

210 215 220

Thr Ser Gly Leu Asp Ser Ala Ser Ala Phe Phe Val Thr Gln Thr Leu

225 230 235 240

Arg Gly Leu Ala Arg Asp Gly Arg Thr Val Ile Ala Ser Val His Gln

245 250 255

Pro Ser Ser Glu Val Phe Leu Leu Phe Asp Cys Leu Tyr Leu Leu Ser

260 265 270

Gly Gly Arg Thr Val Tyr Phe Gly Lys Ala Ser Glu Ala Cys Glu Phe

275 280 285

Phe Ala Gln Ala Gly Phe Pro Cys Pro Pro Met Arg Asn Pro Ser Asp

290 295 300

His Phe Leu Arg Cys Ile Asn Ser Asp Phe Asp Lys Val Lys Ala Thr

305 310 315 320

Leu Lys Gly Ser Met Lys Ala Lys Phe Glu Arg Ser Asn Asp Asp Asp

325 330 335

Pro Leu Glu Lys Met Thr Thr Ser Glu Ala Met Arg Arg Leu Thr Ser

340 345 350

Tyr Tyr Gln His Ser Gln Cys His Ile Asn Ala Gln Gln Lys Val Asp

355 360 365

Glu Met Ala Arg Leu Lys Gly Thr Val Leu Asp Pro Gly Gly Gly Ser

370 375 380

Gln Ala Ser Phe Leu Met Gln Ala Phe Thr Leu Thr Lys Arg Ser Phe

385 390 395 400

Val Asn Met Ser Arg Asp Phe Gly Tyr Tyr Trp Leu Arg Leu Ile Ile

405 410 415

Tyr Ile Val Val Thr Leu Cys Ile Gly Thr Ile Tyr Leu Asn Val Gly

420 425 430

Thr Gly Tyr Ser Ser Ile Leu Ala Arg Gly Ala Cys Ala Ser Phe Ile

435 440 445

Phe Gly Phe Val Thr Phe Met Ser Ile Gly Gly Phe Pro Ser Phe Val

450 455 460

Glu Asp Met Lys Val Phe Gln Arg Glu Arg Leu Asn Gly His Tyr Gly

465 470 475 480

Val Leu Ala Phe Val Val Ser Asn Thr Val Ser Ala Met Pro Phe Leu

485 490 495

Val Leu Ile Thr Phe Val Ser Gly Thr Leu Cys Tyr Phe Met Val Arg

500 505 510

Leu His Pro Gly Phe Thr His Tyr Leu Phe Phe Val Leu Ala Leu Tyr

515 520 525

Ala Ser Val Thr Val Val Glu Ser Leu Met Met Ala Ile Ala Ser Val

530 535 540

Ile Pro Asn Phe Leu Met Gly Ile Ile Ile Gly Ala Gly Ile Gln Gly

545 550 555 560

Ile Phe Met Leu Val Ser Gly Tyr Phe Arg Leu Pro His Asp Ile Pro

565 570 575

Lys Pro Phe Trp Arg Tyr Pro Met Ser Tyr Ile Ser Phe His Tyr Trp

580 585 590

Ala Leu Gln Gly Gln Tyr Gln Asn Asp Leu Arg Gly Leu Leu Phe Asp

595 600 605

Asn Gln Asp Asp Glu Leu Pro Arg Ile Pro Gly Glu Phe Ile Leu Glu

610 615 620

Thr Val Phe Gln Ile Asp Val Ala Arg Ser Lys Trp Leu Asp Leu Ala

625 630 635 640

Val Leu Phe Ser Met Ile Val Ile Tyr Arg Leu Leu Phe Phe Leu Met

645 650 655

Ile Lys Ala Ser Glu Asp Val Thr Pro Trp Leu Arg Arg Tyr Val Ala

660 665 670

Arg Arg Arg Val Arg Asn Arg Arg His Arg Lys Pro Asp Leu Pro Ala

675 680 685

Arg Thr Pro Ser Leu Arg Gly Tyr Val Val Asp Ala Ala Ser Leu Pro

690 695 700

Asp Asp His Pro

705

<210> 19

<211> 2154

<212> DNA

<213> millet (Setaria italica)

<400> 19

atgaggaagg ggggagcggc tgtcggggcg gcgatggtgg ggagggaggc ggcggtcgcg 60

gcggtggctg cggagctgga ggactcggcg gggaaggcgg cgggggcggc ggcgcccgcg 120

ctgagcccgc tcagcgagac gctgtggcgg gacagggcgg cggcgggcgg ggtcctcctc 180

ggtgacgtgt cggcgcgact ggcctggcgg gacctcgccg tcaccgtcgc gctcggcacc 240

ggcgacacgc aggccgtgct gcagggcctc acgggccacg ccgagccggg caccatcacg 300

gcgctcatgg ggccctccgg ctccggcaag tccacgctcc tcgacgcgct cgccgggcgg 360

ctcgccgcca acgccttcct ctccgggacc gtcctcctca acggacgcaa ggccaacctc 420

tccttcggcg ccgcggccta tgtgacgcaa gatgacaacc ttatcggcac attgacggtt 480

cgggagacga tctcatactc ggcgcgcctc cgtctccctg acaagatgcc ctgggaggag 540

aagcaggccc tggtagaggg caccatcatc gagatggggc tgcaggactg tgccgacacg 600

gtcgtcggca actggcacct gaggggcatc agtggtggtg aaaagaggag ggtcagcatt 660

gcccttgaga tactgatgag gcccaggctg ctcttcctgg acgagccaac cagtggtctc 720

gatagcgctt cagcattctt cgtgacacag acactgcgtg ggctggcgag ggacggccgg 780

acggtcatcg cgtccgtgca tcagccgagc agtgaggtgt tcacgctctt tgactgtctg 840

tatcttctat cgggagggaa aactgtctac tttgggaagg cctctgaggc ttgtgagttc 900

ttcgcccaag ctggtttccc gtgcccacca atgcgcaacc cctcggatca ttttctcagg 960

tgcataaatt cggattttga taaggtgaag gccactctga aaggatcaat gaagacgaga 1020

tttgaaaggt ctgatgatcc gctcgagaaa atcacaactt cggaggcaat gaggaggctg 1080

atcaactact accagcactc acagtaccac atcaatgcgc agcagaaagt agatgagatg 1140

gcccgggtta aaggaacagt gctggactca ggagggagcc aagctagttt cgcaatgcag 1200

gcgttcacac tcacgaagcg atcattcatc aacatgtcaa gggattttgg atactactgg 1260

ttgaggctta tcatctacat cgtcgtgaca ctctgcattg ggactatcta cctaaatgtt 1320

ggcactggat acagctccat tctggctcgg ggcgcatgtg cttccttcgt atttggcttt 1380

gtcacattca tgtcgattgg agggtttcca tcgtttgtcg aggacatgaa ggtctttcaa 1440

agggagaggc tcaatgggca ctacggtgtg ctggcgtttg tcatcagcaa cacgctgtca 1500

gcgatgccgt tcctgatcct gatcaccttc gtgtcgggca cgctgtgcta cttcatggtg 1560

cgcctccacc cgggcttcat gcactacctc ttcttcgtcc tggccctcta tgccagcgtc 1620

actgttgtcg agagcctgat gatggccatt gccagcgtga tccccaactt cctcatgggc 1680

atcattatcg gcgcagggat tcagggcata ttcatgcttg tatcaggcta cttcaggctt 1740

ccacatgata tcccaaagcc tttctggagg taccccatgt catatatcag cttccactac 1800

tgggcattgc agggccagta ccagaacgat ctcaagggcc tggtgttcga caaccaggac 1860

gacgagctgc ccaagatccc cggggagtac atcctggaga acgtgttcca gatcgacgtg 1920

aaccggtcca agtggctgga cctggccgtg ctgttcagca tgatcgtcat ctaccgcctc 1980

ctcttcttcg tcatgatcaa gatcagcgag gacgtgaccc cctgggtgcg cggctacatc 2040

gcgcggcggc gggtgcagaa ccggcggcag cgcaaggtgg agctggccgc ccggtcgccg 2100

tcgctccgcg ggtacgtggt ggacgtggcc agcctgccgg ccgaccaccc ctga 2154

<210> 20

<211> 717

<212> PRT

<213> millet (Setaria italica)

<400> 20

Met Arg Lys Gly Gly Ala Ala Val Gly Ala Ala Met Val Gly Arg Glu

1 5 10 15

Ala Ala Val Ala Ala Val Ala Ala Glu Leu Glu Asp Ser Ala Gly Lys

20 25 30

Ala Ala Gly Ala Ala Ala Pro Ala Leu Ser Pro Leu Ser Glu Thr Leu

35 40 45

Trp Arg Asp Arg Ala Ala Ala Gly Gly Val Leu Leu Gly Asp Val Ser

50 55 60

Ala Arg Leu Ala Trp Arg Asp Leu Ala Val Thr Val Ala Leu Gly Thr

65 70 75 80

Gly Asp Thr Gln Ala Val Leu Gln Gly Leu Thr Gly His Ala Glu Pro

85 90 95

Gly Thr Ile Thr Ala Leu Met Gly Pro Ser Gly Ser Gly Lys Ser Thr

100 105 110

Leu Leu Asp Ala Leu Ala Gly Arg Leu Ala Ala Asn Ala Phe Leu Ser

115 120 125

Gly Thr Val Leu Leu Asn Gly Arg Lys Ala Asn Leu Ser Phe Gly Ala

130 135 140

Ala Ala Tyr Val Thr Gln Asp Asp Asn Leu Ile Gly Thr Leu Thr Val

145 150 155 160

Arg Glu Thr Ile Ser Tyr Ser Ala Arg Leu Arg Leu Pro Asp Lys Met

165 170 175

Pro Trp Glu Glu Lys Gln Ala Leu Val Glu Gly Thr Ile Ile Glu Met

180 185 190

Gly Leu Gln Asp Cys Ala Asp Thr Val Val Gly Asn Trp His Leu Arg

195 200 205

Gly Ile Ser Gly Gly Glu Lys Arg Arg Val Ser Ile Ala Leu Glu Ile

210 215 220

Leu Met Arg Pro Arg Leu Leu Phe Leu Asp Glu Pro Thr Ser Gly Leu

225 230 235 240

Asp Ser Ala Ser Ala Phe Phe Val Thr Gln Thr Leu Arg Gly Leu Ala

245 250 255

Arg Asp Gly Arg Thr Val Ile Ala Ser Val His Gln Pro Ser Ser Glu

260 265 270

Val Phe Thr Leu Phe Asp Cys Leu Tyr Leu Leu Ser Gly Gly Lys Thr

275 280 285

Val Tyr Phe Gly Lys Ala Ser Glu Ala Cys Glu Phe Phe Ala Gln Ala

290 295 300

Gly Phe Pro Cys Pro Pro Met Arg Asn Pro Ser Asp His Phe Leu Arg

305 310 315 320

Cys Ile Asn Ser Asp Phe Asp Lys Val Lys Ala Thr Leu Lys Gly Ser

325 330 335

Met Lys Thr Arg Phe Glu Arg Ser Asp Asp Pro Leu Glu Lys Ile Thr

340 345 350

Thr Ser Glu Ala Met Arg Arg Leu Ile Asn Tyr Tyr Gln His Ser Gln

355 360 365

Tyr His Ile Asn Ala Gln Gln Lys Val Asp Glu Met Ala Arg Val Lys

370 375 380

Gly Thr Val Leu Asp Ser Gly Gly Ser Gln Ala Ser Phe Ala Met Gln

385 390 395 400

Ala Phe Thr Leu Thr Lys Arg Ser Phe Ile Asn Met Ser Arg Asp Phe

405 410 415

Gly Tyr Tyr Trp Leu Arg Leu Ile Ile Tyr Ile Val Val Thr Leu Cys

420 425 430

Ile Gly Thr Ile Tyr Leu Asn Val Gly Thr Gly Tyr Ser Ser Ile Leu

435 440 445

Ala Arg Gly Ala Cys Ala Ser Phe Val Phe Gly Phe Val Thr Phe Met

450 455 460

Ser Ile Gly Gly Phe Pro Ser Phe Val Glu Asp Met Lys Val Phe Gln

465 470 475 480

Arg Glu Arg Leu Asn Gly His Tyr Gly Val Leu Ala Phe Val Ile Ser

485 490 495

Asn Thr Leu Ser Ala Met Pro Phe Leu Ile Leu Ile Thr Phe Val Ser

500 505 510

Gly Thr Leu Cys Tyr Phe Met Val Arg Leu His Pro Gly Phe Met His

515 520 525

Tyr Leu Phe Phe Val Leu Ala Leu Tyr Ala Ser Val Thr Val Val Glu

530 535 540

Ser Leu Met Met Ala Ile Ala Ser Val Ile Pro Asn Phe Leu Met Gly

545 550 555 560

Ile Ile Ile Gly Ala Gly Ile Gln Gly Ile Phe Met Leu Val Ser Gly

565 570 575

Tyr Phe Arg Leu Pro His Asp Ile Pro Lys Pro Phe Trp Arg Tyr Pro

580 585 590

Met Ser Tyr Ile Ser Phe His Tyr Trp Ala Leu Gln Gly Gln Tyr Gln

595 600 605

Asn Asp Leu Lys Gly Leu Val Phe Asp Asn Gln Asp Asp Glu Leu Pro

610 615 620

Lys Ile Pro Gly Glu Tyr Ile Leu Glu Asn Val Phe Gln Ile Asp Val

625 630 635 640

Asn Arg Ser Lys Trp Leu Asp Leu Ala Val Leu Phe Ser Met Ile Val

645 650 655

Ile Tyr Arg Leu Leu Phe Phe Val Met Ile Lys Ile Ser Glu Asp Val

660 665 670

Thr Pro Trp Val Arg Gly Tyr Ile Ala Arg Arg Arg Val Gln Asn Arg

675 680 685

Arg Gln Arg Lys Val Glu Leu Ala Ala Arg Ser Pro Ser Leu Arg Gly

690 695 700

Tyr Val Val Asp Val Ala Ser Leu Pro Ala Asp His Pro

705 710 715

<210> 21

<211> 3762

<212> DNA

<213> bis-fringe false bromegrass (Brachypodium distachyon)

<400> 21

atgaagggag ctgaggcggc ggcggcggcg aaggcggagc tggaggacgt ggggaaggcg 60

gcggtccggg cggcggcgcc cccgctgagc ccgctgagcg agacgctgtg gcgggacaag 120

gcggggctcg tcttgctcgg ggacgtgtcg gcgcggctag cgtggcggga cctcacggtg 180

accgtgccgg cggcgggcgg cggcgggacg caggcggtgc tggaggcgct gacggggtac 240

gcggagccgg gcaccatgac ggcgctcatg gggccctccg gctccggcaa gtccactctc 300

ctcgacgcgc tcgccggccg cctcgccgcc aacgcgtttc tatctggcac cgtcctcctc 360

aacgggcgca aggccaacct ctccttcggc gccgcggtac gtaccctgca tatgcctcgc 420

cgtccacggc tagctagcta ctgtaactgg ctgccgctct ctcgctcgat ctcttaatta 480

agctagcttc cctgctagta ggtatactgg cgtactgatc tttagacggt agagtgtact 540

ctgtagtctg tagtgtacat caacaagcaa caagaggtcc ggttctatta aagattcatg 600

catttttcat caacctttta aaagtacatt tttttccctc tacatacata tatccaacaa 660

agaatcctgt gcaatcgaac atatttgttc gtcaatccca tgactttcta ggtagatttg 720

ggcatacaac acggtagcag tttggtgatg gtatacgact agctagcccg ttttactata 780

atcagtctgt ttttcttctt cttttacttc cattccttat ctgtgtttct ccccatgggc 840

catcttgaat tgtgattgaa tctctctgcg ccacctgaga tggcaaccat gcgatgctca 900

caaaccagaa agaagtaatg gaccatgtgc cgcgtggcag gttgtttgga accttgctgg 960

ttcttggctt ctgcctgctc agcttagctg catttgctat ttgttggagg ttatctccta 1020

attgactgca catgaactgc ttctgtctgg acacggatta gcaagttcct agtctcaatt 1080

tgagatctca catcttcttt aatctgggcg tcttgtttgg tgatcgaatt tgacacccat 1140

tttttctaga ggatttttag ttcccatttt ggttggttgg taaagggatg caaggcactt 1200

tttttgtacc tgataagtgg caactggaag cagatgggtt atggttcacc agtttttttt 1260

tcctcaggag tctgctcata ggaaactcat gacaccttca gaacatagta ttctttttgt 1320

ctgcagtgtg aaaattcagt ttctgtttgt taacgagaag tgtgtgtgca tgcgtatgcg 1380

tgtgtctatg ttacctgaaa ccattaatca gatgcatgca atttgtgatt cgggcaggca 1440

tatgtgacgc aagatgacaa tctgatgggc acactgacgg tgagggagac gatctcatac 1500

tcggcgagcc ttcggctccc tgacaagatg cccatggaag agaagcgtga cctggtggag 1560

ggcacaatcg tggagatggg gctccaggac tgcgccgaca cggtcatcgg caattggcac 1620

ctcagggggg tcagcggcgg tgagaagaga agggtcagca tcgccctgga gatcctcatg 1680

aggcctaggc tgctcttcct ggatgagccc accagtggtc ttgacaggta tatatggcat 1740

cttcacaatt tcagtcatgc aattcttttt attcagttgt ttgtaaatat tttgctcgca 1800

tacaagttcg ataatgatac atatctgaat tctgaaaact atgcttgtcg tgcagcgctt 1860

cagcgttctt cgtgacgcag acgctccgcg ggctggcgag ggatgggcgg acagtgatcg 1920

cgtcgatcca tcagccgagc agcgaggtct tccagctctt cgatcgcctg tatcttcttt 1980

cagggggcaa aacagtctac tttgggcagg cgtctgaggc ttgtgaggta ctctgtagct 2040

taatcttttt atctgtattt cttcaatgcc aaattgccaa tgccaatggg gtctctcaag 2100

cagtctgaat atgcaatgca gttctttgcc caagctggct tcccatgccc agcactacgc 2160

aacccgtcgg atcatttcct ccgctgcata aacgcagact tcgacaaggt gaaggccacc 2220

ctgaaaggat caatgaagat gagagtaagt ttcttcagcc actatttcac tgatgaatct 2280

caattagctt tctgatggac cttaattagt ttgagaggtc cgacgatccg ctagagcgca 2340

tcacgacctg cgaggcaatc agaaagttat tcagccacta ccagcactcg caggactacc 2400

tcgccacccg gcagaaagtg gacgagatgg cacgggtcgt aagtgcagct gatccatttt 2460

tcttcagaaa cctacctgca atgacaagta aattacgatg gttcattttg catctgcaga 2520

aaggaacggt cctggacgca gggggcagcc aggccagctt cgggatgcaa gcctgcacgc 2580

tgacgaagcg gtccttcgtg aacatgacga gggacttcgg gtactactgg ctgcggctgg 2640

tgatctacgt ggtggtcacc gtgtgcatcg gctccatcta cctcaacgtg ggcaccaagt 2700

acagctccat cctggcgcgg ggcgcgtgcg cgtccttcat cttcggcttc gtcaccttca 2760

tgtccatcgg gggcttcccc tccttcgtgg aggacatgaa ggtgttccag cgggagcggc 2820

tcaacggcca ctacggcgtg gccgcgttcg tgctcggcaa cacggcgtcc gcggcgccgt 2880

tcctgctgct catcaccgtc tgctccggga cgctatgcta cttcatggtg gggctacacc 2940

cggggctcag ccattatgtt ttcttcctgc tctgcctcta tgccagcgtc accgtcgtcg 3000

agagcctcat gatggccatc gccagcgtcg tcccgaattt tctcatgggc atcatcaccg 3060

gcgccggaat tcaaggggtg ttcatgctcg tctcggggta tttccgcctc ccccacgaca 3120

tccccaagcc cttctggcgt taccccatgt catacatcag cttccactac tgggccctgc 3180

aggtaacatc cagatcgatt tttccatctt tcctctgaat gaagcgtcaa gatcctgcat 3240

gcacgcatgg tgtttgatgg attgattttg caggggcagt accagaacga tttggtgggg 3300

ctcatgttcg acaaccagag cgagttgctg cccaagatcc cgggggagta cgtgctggag 3360

aacgtgttcc agatcgacgt ggggaggtca aagtggctgg acttgtccgt gcttttcgcc 3420

atgatcgtcg tctaccgcct gctcttcttc gccatgatca aggtcagcga ggacgtgacg 3480

ccgtggctgc gccggtacat cgccaggagg agggtgcagc gcaggcgagg ccgggcccgg 3540

gacgcggcgg cggagctcca agttggccgc tcgccgtcgc tgcgcgggta cgtcgtcgac 3600

gacgacctgc cggccgatca tccgtgacag tatgagctca agaagaaagg ttaattggtt 3660

ggacagacga aattaacgct aaaataactg gacgataaaa cagtctgata tgaatgatgt 3720

actcgtatgt gtgttagcta gggcatctcc gatttgttct ct 3762

<210> 22

<211> 2127

<212> DNA

<213> bis-fringe false bromegrass (Brachypodium distachyon)

<400> 22

atgaagggag ctgaggcggc ggcggcggcg aaggcggagc tggaggacgt ggggaaggcg 60

gcggtccggg cggcggcgcc cccgctgagc ccgctgagcg agacgctgtg gcgggacaag 120

gcggggctcg tcttgctcgg ggacgtgtcg gcgcggctag cgtggcggga cctcacggtg 180

accgtgccgg cggcgggcgg cggcgggacg caggcggtgc tggaggcgct gacggggtac 240

gcggagccgg gcaccatgac ggcgctcatg gggccctccg gctccggcaa gtccactctc 300

ctcgacgcgc tcgccggccg cctcgccgcc aacgcgtttc tatctggcac cgtcctcctc 360

aacgggcgca aggccaacct ctccttcggc gccgcggcat atgtgacgca agatgacaat 420

ctgatgggca cactgacggt gagggagacg atctcatact cggcgagcct tcggctccct 480

gacaagatgc ccatggaaga gaagcgtgac ctggtggagg gcacaatcgt ggagatgggg 540

ctccaggact gcgccgacac ggtcatcggc aattggcacc tcaggggggt cagcggcggt 600

gagaagagaa gggtcagcat cgccctggag atcctcatga ggcctaggct gctcttcctg 660

gatgagccca ccagtggtct tgacagcgct tcagcgttct tcgtgacgca gacgctccgc 720

gggctggcga gggatgggcg gacagtgatc gcgtcgatcc atcagccgag cagcgaggtc 780

ttccagctct tcgatcgcct gtatcttctt tcagggggca aaacagtcta ctttgggcag 840

gcgtctgagg cttgtgagtt ctttgcccaa gctggcttcc catgcccagc actacgcaac 900

ccgtcggatc atttcctccg ctgcataaac gcagacttcg acaaggtgaa ggccaccctg 960

aaaggatcaa tgaagatgag atttgagagg tccgacgatc cgctagagcg catcacgacc 1020

tgcgaggcaa tcagaaagtt attcagccac taccagcact cgcaggacta cctcgccacc 1080

cggcagaaag tggacgagat ggcacgggtc aaaggaacgg tcctggacgc agggggcagc 1140

caggccagct tcgggatgca agcctgcacg ctgacgaagc ggtccttcgt gaacatgacg 1200

agggacttcg ggtactactg gctgcggctg gtgatctacg tggtggtcac cgtgtgcatc 1260

ggctccatct acctcaacgt gggcaccaag tacagctcca tcctggcgcg gggcgcgtgc 1320

gcgtccttca tcttcggctt cgtcaccttc atgtccatcg ggggcttccc ctccttcgtg 1380

gaggacatga aggtgttcca gcgggagcgg ctcaacggcc actacggcgt ggccgcgttc 1440

gtgctcggca acacggcgtc cgcggcgccg ttcctgctgc tcatcaccgt ctgctccggg 1500

acgctatgct acttcatggt ggggctacac ccggggctca gccattatgt tttcttcctg 1560

ctctgcctct atgccagcgt caccgtcgtc gagagcctca tgatggccat cgccagcgtc 1620

gtcccgaatt ttctcatggg catcatcacc ggcgccggaa ttcaaggggt gttcatgctc 1680

gtctcggggt atttccgcct cccccacgac atccccaagc ccttctggcg ttaccccatg 1740

tcatacatca gcttccacta ctgggccctg caggggcagt accagaacga tttggtgggg 1800

ctcatgttcg acaaccagag cgagttgctg cccaagatcc cgggggagta cgtgctggag 1860

aacgtgttcc agatcgacgt ggggaggtca aagtggctgg acttgtccgt gcttttcgcc 1920

atgatcgtcg tctaccgcct gctcttcttc gccatgatca aggtcagcga ggacgtgacg 1980

ccgtggctgc gccggtacat cgccaggagg agggtgcagc gcaggcgagg ccgggcccgg 2040

gacgcggcgg cggagctcca agttggccgc tcgccgtcgc tgcgcgggta cgtcgtcgac 2100

gacgacctgc cggccgatca tccgtga 2127

<210> 23

<211> 708

<212> PRT

<213> bis-fringe false bromegrass (Brachypodium distachyon)

<400> 23

Met Lys Gly Ala Glu Ala Ala Ala Ala Ala Lys Ala Glu Leu Glu Asp

1 5 10 15

Val Gly Lys Ala Ala Val Arg Ala Ala Ala Pro Pro Leu Ser Pro Leu

20 25 30

Ser Glu Thr Leu Trp Arg Asp Lys Ala Gly Leu Val Leu Leu Gly Asp

35 40 45

Val Ser Ala Arg Leu Ala Trp Arg Asp Leu Thr Val Thr Val Pro Ala

50 55 60

Ala Gly Gly Gly Gly Thr Gln Ala Val Leu Glu Ala Leu Thr Gly Tyr

65 70 75 80

Ala Glu Pro Gly Thr Met Thr Ala Leu Met Gly Pro Ser Gly Ser Gly

85 90 95

Lys Ser Thr Leu Leu Asp Ala Leu Ala Gly Arg Leu Ala Ala Asn Ala

100 105 110

Phe Leu Ser Gly Thr Val Leu Leu Asn Gly Arg Lys Ala Asn Leu Ser

115 120 125

Phe Gly Ala Ala Ala Tyr Val Thr Gln Asp Asp Asn Leu Met Gly Thr

130 135 140

Leu Thr Val Arg Glu Thr Ile Ser Tyr Ser Ala Ser Leu Arg Leu Pro

145 150 155 160

Asp Lys Met Pro Met Glu Glu Lys Arg Asp Leu Val Glu Gly Thr Ile

165 170 175

Val Glu Met Gly Leu Gln Asp Cys Ala Asp Thr Val Ile Gly Asn Trp

180 185 190

His Leu Arg Gly Val Ser Gly Gly Glu Lys Arg Arg Val Ser Ile Ala

195 200 205

Leu Glu Ile Leu Met Arg Pro Arg Leu Leu Phe Leu Asp Glu Pro Thr

210 215 220

Ser Gly Leu Asp Ser Ala Ser Ala Phe Phe Val Thr Gln Thr Leu Arg

225 230 235 240

Gly Leu Ala Arg Asp Gly Arg Thr Val Ile Ala Ser Ile His Gln Pro

245 250 255

Ser Ser Glu Val Phe Gln Leu Phe Asp Arg Leu Tyr Leu Leu Ser Gly

260 265 270

Gly Lys Thr Val Tyr Phe Gly Gln Ala Ser Glu Ala Cys Glu Phe Phe

275 280 285

Ala Gln Ala Gly Phe Pro Cys Pro Ala Leu Arg Asn Pro Ser Asp His

290 295 300

Phe Leu Arg Cys Ile Asn Ala Asp Phe Asp Lys Val Lys Ala Thr Leu

305 310 315 320

Lys Gly Ser Met Lys Met Arg Phe Glu Arg Ser Asp Asp Pro Leu Glu

325 330 335

Arg Ile Thr Thr Cys Glu Ala Ile Arg Lys Leu Phe Ser His Tyr Gln

340 345 350

His Ser Gln Asp Tyr Leu Ala Thr Arg Gln Lys Val Asp Glu Met Ala

355 360 365

Arg Val Lys Gly Thr Val Leu Asp Ala Gly Gly Ser Gln Ala Ser Phe

370 375 380

Gly Met Gln Ala Cys Thr Leu Thr Lys Arg Ser Phe Val Asn Met Thr

385 390 395 400

Arg Asp Phe Gly Tyr Tyr Trp Leu Arg Leu Val Ile Tyr Val Val Val

405 410 415

Thr Val Cys Ile Gly Ser Ile Tyr Leu Asn Val Gly Thr Lys Tyr Ser

420 425 430

Ser Ile Leu Ala Arg Gly Ala Cys Ala Ser Phe Ile Phe Gly Phe Val

435 440 445

Thr Phe Met Ser Ile Gly Gly Phe Pro Ser Phe Val Glu Asp Met Lys

450 455 460

Val Phe Gln Arg Glu Arg Leu Asn Gly His Tyr Gly Val Ala Ala Phe

465 470 475 480

Val Leu Gly Asn Thr Ala Ser Ala Ala Pro Phe Leu Leu Leu Ile Thr

485 490 495

Val Cys Ser Gly Thr Leu Cys Tyr Phe Met Val Gly Leu His Pro Gly

500 505 510

Leu Ser His Tyr Val Phe Phe Leu Leu Cys Leu Tyr Ala Ser Val Thr

515 520 525

Val Val Glu Ser Leu Met Met Ala Ile Ala Ser Val Val Pro Asn Phe

530 535 540

Leu Met Gly Ile Ile Thr Gly Ala Gly Ile Gln Gly Val Phe Met Leu

545 550 555 560

Val Ser Gly Tyr Phe Arg Leu Pro His Asp Ile Pro Lys Pro Phe Trp

565 570 575

Arg Tyr Pro Met Ser Tyr Ile Ser Phe His Tyr Trp Ala Leu Gln Gly

580 585 590

Gln Tyr Gln Asn Asp Leu Val Gly Leu Met Phe Asp Asn Gln Ser Glu

595 600 605

Leu Leu Pro Lys Ile Pro Gly Glu Tyr Val Leu Glu Asn Val Phe Gln

610 615 620

Ile Asp Val Gly Arg Ser Lys Trp Leu Asp Leu Ser Val Leu Phe Ala

625 630 635 640

Met Ile Val Val Tyr Arg Leu Leu Phe Phe Ala Met Ile Lys Val Ser

645 650 655

Glu Asp Val Thr Pro Trp Leu Arg Arg Tyr Ile Ala Arg Arg Arg Val

660 665 670

Gln Arg Arg Arg Gly Arg Ala Arg Asp Ala Ala Ala Glu Leu Gln Val

675 680 685

Gly Arg Ser Pro Ser Leu Arg Gly Tyr Val Val Asp Asp Asp Leu Pro

690 695 700

Ala Asp His Pro

705

<210> 24

<211> 25

<212> DNA

<213> synthetic

<400> 24

ggctgatcac ctactacaag aactc 25

<210> 25

<211> 20

<212> DNA

<213> synthetic

<400> 25

ctcctgcatc cagcactgtc 20

<210> 26

<211> 20

<212> DNA

<213> synthetic

<400> 26

gctatgtacg tcgccatcca 20

<210> 27

<211> 21

<212> DNA

<213> synthetic

<400> 27

ggacagtgtg gctgacacca t 21

<210> 28

<211> 27

<212> DNA

<213> synthetic

<400> 28

actggtaccc ttaatccctt ctatacc 27

<210> 29

<211> 28

<212> DNA

<213> synthetic

<400> 29

tctgtcgact catctctccc tctctcgc 28

<210> 30

<211> 27

<212> DNA

<213> synthetic

<400> 30

actggtaccc ttaatccctt ctatacc 27

<210> 31

<211> 31

<212> DNA

<213> synthetic

<400> 31

agtggatccg gatcatggaa atatcaagta c 31

<210> 32

<211> 29

<212> DNA

<213> synthetic

<400> 32

ctcagatctg atccaagtgg ctggacctc 29

<210> 33

<211> 31

<212> DNA

<213> synthetic

<400> 33

ctcactagtc agctgctgat atacaccttg a 31

<210> 34

<211> 244

<212> DNA

<213> paddy rice (Oryza sativa Japonica)

<400> 34

agagagaggc tcaatgggca ctatggcgtg ctggcattcg tgataagcaa cacgatctcg 60

gcaatgccat tcttgatctt gatcaccttc atctccggga caatgtgcta cttcatggtg 120

cgcctccacc cgggcttcac gcactacctc ttcttcgtcc tctgcctcta cgcaagcgtc 180

accgtcgtcg agagcttgat gatggccatt gccagtgtca tccccaactt cctcatgggc 240

atca 244

<210> 35

<211> 244

<212> DNA

<213> paddy rice (Oryza sativa Japonica)

<400> 35

tgatgcccat gaggaagttg gggatgacac tggcaatggc catcatcaag ctctcgacga 60

cggtgacgct tgcgtagagg cagaggacga agaagaggta gtgcgtgaag cccgggtgga 120

ggcgcaccat gaagtagcac attgtcccgg agatgaaggt gatcaagatc aagaatggca 180

ttgccgagat cgtgttgctt atcacgaatg ccagcacgcc atagtgccca ttgagcctct 240

ctct 244

<210> 36

<211> 29

<212> DNA

<213> synthetic

<400> 36

ctcagatctg atccaagtgg ctggacctc 29

<210> 37

<211> 31

<212> DNA

<213> synthetic

<400> 37

ctcactagtc agctgctgat atacaccttg a 31

<210> 38

<211> 480

<212> DNA

<213> paddy rice (Oryza sativa Japonica)

<400> 38

gatccaagtg gctggacctc gccgtgctct tcagcatgat cttcatctac cgcctcctct 60

tcttcgccat gatcaaggtc agcgaggacg tcaccccgtg ggtgcgcggc tacgtcgcgc 120

ggcgccgggt gcagggcaag ggcgcccgcg gccgcggggc ggacctctcc gccgcgcgct 180

cgccgtcgct ccgcgcctac gtcgtcgacg ccgccgacga cctgccgccg gcctgaccac 240

ccgtgagaca gacacggctt ctgaaaactc tgaaactctg aactcctcaa ctgaacaagt 300

ggagacagag gaagaaaaag agagagaggg agaggctaaa actgaactaa aaaaaaaaga 360

acctgaatat ctgatatgat aattgtgtgt tatagtagta gcaatgctta attaatcgat 420

caagctgtgt tgttgtattg tattgataat tactaatatc aaggtgtata tcagcagctg 480

<210> 39

<211> 480

<212> DNA

<213> paddy rice (Oryza sativa Japonica)

<400> 39

cagctgctga tatacacctt gatattagta attatcaata caatacaaca acacagcttg 60

atcgattaat taagcattgc tactactata acacacaatt atcatatcag atattcaggt 120

tctttttttt ttagttcagt tttagcctct ccctctctct ctttttcttc ctctgtctcc 180

acttgttcag ttgaggagtt cagagtttca gagttttcag aagccgtgtc tgtctcacgg 240

gtggtcaggc cggcggcagg tcgtcggcgg cgtcgacgac gtaggcgcgg agcgacggcg 300

agcgcgcggc ggagaggtcc gccccgcggc cgcgggcgcc cttgccctgc acccggcgcc 360

gcgcgacgta gccgcgcacc cacggggtga cgtcctcgct gaccttgatc atggcgaaga 420

agaggaggcg gtagatgaag atcatgctga agagcacggc gaggtccagc cacttggatc 480

 

 

Claims (10)

1. a DNA sequence dna, has the function of regulating plant fertility, it is characterized in that, described DNA sequence dna is selected from one of sequence of following group:

There is the nucleotide sequence shown in SEQ ID NO:1 or 5;

There is the nucleotide sequence shown in SEQ ID NO:10 or 11;

There is the nucleotide sequence shown in SEQ ID NO:13 or 14;

There is the nucleotide sequence shown in SEQ ID NO:16 or 17;

There is the nucleotide sequence shown in SEQ ID NO:19;

There is the nucleotide sequence shown in SEQ ID NO:21 or 22;

Under strict conditions can with the DNA sequence dna of the DNA hybridization of the arbitrary described sequence of (a)-(f); Or

With the DNA sequence dna of the arbitrary described complementary of (a)-(g).

2. DNA sequence dna according to claim 1, is characterized in that the aminoacid sequence of described DNA sequence encoding is as shown in SEQ ID NO:2,6,12,15,18,20 or 23.

3. an expression cassette, is characterized in that described expression cassette comprises DNA sequence dna according to claim 1.

4. an expression vector, is characterized in that described expression vector comprises expression cassette according to claim 3.

5. the application of a mutant material, described mutant material caused by the sudden change of nucleotide sequence, male sterile is shown as containing the plant of nucleotide sequence after this sudden change, it is characterized in that the described nucleotide sequence by sudden change generation male sterile phenotype is as shown in SEQ ID NO:1,5,10,11,13,14,16,17,19,21 or 22, described sudden change can be point mutation, also can be DNA disappearance or insertion mutation, also can be produced by the gene silencing such as RNAi, rite-directed mutagenesis means.

6. a promotor, has the characteristic of flower pesticide specifically expressing, it is characterized in that the nucleotide sequence of described promotor is as shown in SEQ ID NO:3 or 9.

7. an expression cassette, is characterized in that described expression cassette comprises promoter sequence according to claim 6.

8. an expression vector, is characterized in that described expression vector comprises expression cassette according to claim 7.

9. in plant, express the method for object nucleotide sequence for one kind, described method comprises to plant materials importing DNA construct, described DNA construct contains the object nucleotide sequence that promotor and operability are connected to described promotor, the nucleotide sequence of wherein said promotor is as shown in SEQ ID NO:3 or 9, described plant optimization is monocotyledons, described monocotyledons is preferably grass, and described grass is preferably paddy rice, corn, Chinese sorghum, barley, millet or two fringe false bromegrasses.

10. a method, for keeping the homozygous recessive condition of male sterile plants, described method comprises:

A () provides the first plant, it comprises the homozygous recessive alleles of FG3 gene, and it is male sterile;

B () introduces following construct in the first plant, form the second plant, and described second plant comprises the homozygous recessive alleles of FG3 gene and described construct, and construct is hemizygous condition in the second plant, and described construct comprises:

I) the first nucleotide sequence, it comprises FG3 nucleotide sequence, and when expressing in the first plant, it will recover male fertility;

Ii) the second nucleotide sequence, when it is expressed, can suppress formation or the function that can educate male gamete in described second plant, be specially pollen inactivated gene ZM-PA; And

C () makes described first plant fertilization, to produce the offspring maintaining described first plant homozygous recessive condition with the male gamete of described second plant.