CN104906072A - Method for preparing Glechoma hedracea volatile oil microcapsule and application thereof - Google Patents
Method for preparing Glechoma hedracea volatile oil microcapsule and application thereof Download PDFInfo
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- CN104906072A CN104906072A CN201510270199.4A CN201510270199A CN104906072A CN 104906072 A CN104906072 A CN 104906072A CN 201510270199 A CN201510270199 A CN 201510270199A CN 104906072 A CN104906072 A CN 104906072A
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Abstract
The invention discloses a method for preparing a glechoma hedracea volatile oil microcapsule and an application thereof. Corn protein and gelatin serve as wall materials, glechoma hedracea volatile oil serves as a core material, and then a glechoma hedracea volatile oil microcapsule is prepared and obtained through high-pressure homogenization, freezing and drying. The obtained glechoma hedracea volatile oil microcapsule is in a powder shape and good in water solubility and stability, the defects that glechoma hedracea volatile oil is not dissolved in water and volatile components are likely to be lost are overcome, and original active components of the glechoma hedracea volatile oil are not prone to being damaged and are slowly released. The method for preparing the glechoma hedracea volatile oil microcapsule is simple, the yield of the microcapsule is high, entrapment efficiency and the drug-loading rate are high, remarkable anti-bacterial activity is achieved, and the method can be applied to air conditioner formulas.
Description
Technical field
The present invention relates to a kind of preparation method and application thereof of Herba Glechomae volatile oil microcapsule.
Background technology
Herba Glechomae is the dry herb of Labiatae Glechoma plant HUOXUEDAN.Dry or using fresh herb.Herba Glechomae is among the people for diuresis and expelling stone in Jiangsu, also known as " HERBA GLECHOMAE ", cures mainly the wet pouring of pyretic stranguria, jaundice due to damp-heat, sore, carbuncle and painful swelling, traumatic injury.Be mainly used in diuresis clinically, treatment vesical calculus, renal calculus, ureter, oedema due to nephritis, cholecystitis, cholelithiasis, parotitis, burn etc.Show through bacteriostatic experiment, Herba Glechomae extract to staphylococcus aureus extreme sensitivity, to dysentery bacterium, bacillus pyocyaneus, Bacillus typhi medium sensitivity.At present, the chemical research of Herba Glechomae is only limitted to volatile ingredient, less to its volatility pharmacological research.
Volatile oil is the effective constituents in Chinese medicine, widely distributed in plant kingdom, and has multiple pharmacologically active and purposes widely.Be mainly used in varieties of food items, cosmetics of everyday use, making essence to use, to the not yet systematic expansion of the functional study of volatile oil.Now to volatile oil research more be the antioxidant activity of volatile oil, and relatively less to its antibacterial activity research.Therefore, to the research of the antibacterial action of Herba Glechomae volatile oil, contribute to the deep development to Herba Glechomae volatile oil, as anticorrosion in food antiseptic, cosmetics of everyday use etc.
Due to the high volatility of volatile oil, easily oxidized, cause it to preserve and use all being very restricted.Utilize microcapsule technology that Herba Glechomae volatile oil is carried out micro encapsulation, greatly can improve the stable of product, be convenient to storage and utilize.
Microcapsule technology is a kind of a kind of resist technology adopting filmogen some to be had reactivity, sensitivity or volatile liquid or solid encapsulating formation fine particle; the process of encapsulating is micro encapsulation; the fine particle formed is called microcapsule; size is generally at 1 ~ 1000 μm, and the thickness of cyst wall is at 0.2 ~ 10 μm.The advantage of technology be can protective core materials/substances from the impact of environmental condition, reduce oxidation, passivation heliosensitivity and thermal sensitivity, reduce core volatility.
By literature search, identical with the present invention open source literature report is had no.
Summary of the invention
The object of the present invention is to provide a kind of preparation method and application of Herba Glechomae volatile oil microcapsule, this microcapsule has good stability and dissolubility, and antibacterial effect is obvious.
The present invention completes by following technical solution:
A preparation method for Herba Glechomae volatile oil microcapsule, comprises the steps:
A. by zein water dissolution, preparation quality volume ratio is the zein aqueous solution of 5 ~ 8%, regulates pH to 10.0, then with acetic acid, solution is adjusted to pH8.0 with sodium bicarbonate;
B. gelatin is soluble in water, be prepared into the colloidal solution that mass volume ratio is 3 ~ 6%, be adjusted to pH4.0 with acetic acid;
C. Herba Glechomae volatile oil is joined in zein aqueous solution, the colloidal solution of gained in step b is added after stirring, high pressure homogenizer is passed into after 10000 ~ 15000r/min high-speed stirred 10min, homogenizing three times under the condition of temperature 70 ~ 80 DEG C with pressure 30 ~ 50MPa, forms the mixture of uniform particle diameter;
D. by mixture lyophilization in freeze drying box after 0 DEG C of cooling 24h, pulverous Herba Glechomae volatile oil microcapsule is obtained.
Described microcapsule quality proportioning is: Herba Glechomae volatile oil proportion is 20 ~ 30 weight portions, and zein is 30 ~ 40 weight portions, and gelatin is 30 ~ 50 weight portions;
Described lyophilization condition is: condensation temperature-50 ~-60 DEG C, pressure 0.2 ~ 0.5KPa, and finishing temperature is 10 ~ 20 DEG C.
The invention provides a kind of hair conditioner formula containing above-mentioned microcapsule.
In hair conditioner formula, the weight percent content of described Herba Glechomae volatile oil microcapsule is 0.1 ~ 0.5%.
Tool of the present invention has the following advantages and effect:
(1) the technology of the present invention route simple possible, mild condition, suitability for industrialized is produced;
(2) wall material of the present invention selects zein and gelatin, not only has no side effect, and has biological degradability, can not produce environmental pollution.Herba Glechomae volatile oil, by micro encapsulation, separates with air, avoids oxidation and the volatilization of volatile ingredient, can improve its stability, maintain the reactivity that it is good, can not affect other performance.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to following embodiments.
detailed description of the invention:
Embodiment 1:
By 30g zein water dissolution, preparation quality volume ratio is the zein aqueous solution of 5%, regulates pH to 10.0, then with acetic acid, solution is adjusted to pH8.0 with sodium bicarbonate; By soluble in water for 50g gelatin, be prepared into the colloidal solution that mass volume ratio is 6%, be adjusted to pH4.0 with acetic acid; 20g Herba Glechomae volatile oil is joined in zein aqueous solution, the colloidal solution of gained is added after stirring, after 15000r/min high-speed stirred 10min, pass into high pressure homogenizer, homogenizing three times under the condition of temperature 80 DEG C with pressure 50MPa, forms the mixture of uniform particle diameter; Condensation temperature-60 DEG C after mixture is cooled 24h in 0 DEG C, pressure 0.5KPa, finishing temperature is lyophilization under 20 DEG C of conditions, obtains pulverous Herba Glechomae volatile oil microcapsule.
Embodiment 2:
By 35g zein water dissolution, preparation quality volume ratio is the zein aqueous solution of 8%, regulates pH to 10.0, then with acetic acid, solution is adjusted to pH8.0 with sodium bicarbonate; By soluble in water for 40g gelatin, be prepared into the colloidal solution that mass volume ratio is 3%, be adjusted to pH4.0 with acetic acid; 25g Herba Glechomae volatile oil is joined in zein aqueous solution, the colloidal solution of gained is added after stirring, after 10000r/min high-speed stirred 10min, pass into high pressure homogenizer, homogenizing three times under the condition of temperature 70 C and pressure 30MPa, forms the mixture of uniform particle diameter; Condensation temperature-50 DEG C after mixture is cooled 24h in 0 DEG C, pressure 0.4KPa, finishing temperature is lyophilization under 15 DEG C of conditions, obtains pulverous Herba Glechomae volatile oil microcapsule.
Embodiment 3:
By 40g zein water dissolution, preparation quality volume ratio is the zein aqueous solution of 6%, regulates pH to 10.0, then with acetic acid, solution is adjusted to pH8.0 with sodium bicarbonate; By soluble in water for 30g gelatin, be prepared into the colloidal solution that mass volume ratio is 4%, be adjusted to pH4.0 with acetic acid; 30g Herba Glechomae volatile oil is joined in zein aqueous solution, the colloidal solution of gained is added after stirring, after 12000r/min high-speed stirred 10min, pass into high pressure homogenizer, homogenizing three times under the condition of temperature 75 DEG C with pressure 40MPa, forms the mixture of uniform particle diameter; Condensation temperature-55 DEG C after mixture is cooled 24h in 0 DEG C, pressure 0.2KPa, finishing temperature is lyophilization under 10 DEG C of conditions, obtains pulverous Herba Glechomae volatile oil microcapsule.
Embodiment 4: the fungistatic effect of Herba Glechomae volatile oil microcapsule
By above-mentioned gained volatile oil microcapsule by weight percentage content be 0.1 ~ 0.5% to join in hair conditioner formula.By comparing Herba Glechomae volatile oil and microcapsule product thereof and the hair conditioner containing this microcapsule suppression ratio to escherichia coli and staphylococcus aureus, evaluate the inhibition of this microcapsule product to pathogenic bacterium.Adopt flat band method, on the nutrient agar containing variable concentrations antibacterial, after 37 DEG C of above-mentioned 2 kinds of bacterium 24h of culture medium, detect its clump count.The bacterium colony concentration of the experimental strain in this experiment is 10
5cFU/mL.Do not add the flat board contrast of any antibacterial.The results are shown in Table 1:
The computing formula of bacteriostasis rate: suppression ratio=(1-test group bacterium colony/matched group bacterium colony) × 100%
The Herba Glechomae volatile oil of table 1 variable concentrations and the suppression ratio of microcapsule product
Experiment shows, five groups of antibacterial contain the Herba Glechomae volatile oil of same concentrations, and as can be seen from the table, after microcapsule embedded, the fungistatic effect of Herba Glechomae volatile oil does not reduce significantly, and can well be applied in hair conditioner product, antiseptic effect is obvious.
Claims (5)
1. a preparation method for Herba Glechomae volatile oil microcapsule, is characterized in that, the method comprises the Herba Glechomae volatile oil microcapsule based on 100 weight portions:
A. by zein water dissolution, preparation quality volume ratio is the zein aqueous solution of 5 ~ 8%, regulates pH to 10.0, then with acetic acid, solution is adjusted to pH8.0 with sodium bicarbonate;
B. gelatin is soluble in water, be prepared into the colloidal solution that mass volume ratio is 3 ~ 6%, be adjusted to pH4.0 with acetic acid;
C. Herba Glechomae volatile oil is joined in zein aqueous solution, the colloidal solution of gained in step b is added after stirring, high pressure homogenizer is passed into after 10000 ~ 15000r/min high-speed stirred 10min, homogenizing three times under the condition of temperature 70 ~ 80 DEG C with pressure 30 ~ 50MPa, forms the mixture of uniform particle diameter;
D. by mixture lyophilization in freeze drying box after 0 DEG C of cooling 24h, pulverous Herba Glechomae volatile oil microcapsule is obtained.
2. the preparation method of a kind of Herba Glechomae volatile oil microcapsule according to claim 1, it is characterized in that, described microcapsule quality proportioning is: Herba Glechomae volatile oil proportion is 20 ~ 30 weight portions, and zein is 30 ~ 40 weight portions, and gelatin is 30 ~ 50 weight portions;
The preparation method of a kind of Herba Glechomae volatile oil microcapsule according to claim 1, is characterized in that, the lyophilization condition described in steps d is: condensation temperature-50 ~-60 DEG C, pressure 0.2 ~ 0.5KPa, and finishing temperature is 10 ~ 20 DEG C.
3. the Herba Glechomae volatile oil microcapsule obtained according to the preparation method described in claim 1 ~ 3.
4. the hair conditioner formula containing Herba Glechomae volatile oil microcapsule as described in claim 4.
5. hair conditioner formula according to claim 5, is characterized in that, the weight percent content of described Herba Glechomae volatile oil microcapsule is 0.1 ~ 0.5%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737309A (en) * | 2017-11-28 | 2018-02-27 | 戴凤 | A kind of composite antibacterial microcapsules and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737309A (en) * | 2017-11-28 | 2018-02-27 | 戴凤 | A kind of composite antibacterial microcapsules and preparation method thereof |
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Application publication date: 20150916 |