CN104904482A - Cymbidium goeringii rhizome growing method - Google Patents
Cymbidium goeringii rhizome growing method Download PDFInfo
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- CN104904482A CN104904482A CN201510340452.9A CN201510340452A CN104904482A CN 104904482 A CN104904482 A CN 104904482A CN 201510340452 A CN201510340452 A CN 201510340452A CN 104904482 A CN104904482 A CN 104904482A
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- chunlan
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention relates to a cymbidium goeringii rhizome growing method, and belongs to the field of plant biotechnologies. The cymbidium goeringii rhizome growing method comprises the four steps of material preparation, pretreatment, cultivation and management and protection. An appropriate planting pot, pot soil and cymbidium goeringii are selected, the corm of the cymbidium goeringii makes direct contact with the pot bottom of the planting pot in the planting process, then management and protection for about two months are conducted, and cymbidium goeringii rhizome growth can be seen on the corm of the cymbidium goeringii. The cymbidium goeringii rhizome growing method overcomes the defect that the rhizome obtained through seed germination cannot completely inherit the excellent characteristics of the male and female parent in the prior art, the method also overcomes the defects that when stem tip induction is used for obtaining the rhizome in the prior art, cultivation difficulty is large and time is long, hence, it is guaranteed that the grown rhizome can completely inherit the excellent characteristics of the male and female parent, the rhizome can be grown out quickly and conveniently, and the method is suitable for industrialization production of factories.
Description
Technical field
The present invention relates to plant biotechnology field, particularly relate to the rhizomatic growing method of a kind of Chunlan.
Background technology
Chunlan belongs to the orchid family (Orchidaceae) plant in Plant Taxonomy, has higher ornamental value and economic worth.The Chunlan (Cymbidium goeringii) of China's cultivation is the Terrestrial orchid in the orchid family (Orchidaceae) Cymbidium (Cymbidium) plant, cultivation history is long, become with its light far unsurpassably wonderful delicate fragrance and simple and elegant white and pure flower and spend middle treasure and enjoy praise, germ plasm resource is very abundant, and market demand is very large.Along with the development of modern biotechnology, be that the biotechnology of Main Means is progressively applied in the reproductive process of Chunlan with tissue cultures, it is generally acknowledged that orchid tissue cultures has two approach: one is form protocorm by the non-symbiosis germination of seed, and two is for explant cultivates induced synthesis protocorms with stem apex, lateral bud etc.The protocorm of Chunlan is owing to looking like root shape, and not quite alike with other orchids, spy is referred to as root-like stock.
People find the root-like stock formed by seed germination by research, formed, have the genotype of Parent by globular embryo direct development in seed; Explant, mainly stem apex, the root-like stock that induction produces mainly under stem apex epidermis or epidermis one layer of cells there is the embryo parenchyma cell of meristematic capacity, cell dedifferentiation can form cells,primordial under certain conditions, then comes through spherical embryonic development.
But the root-like stock formed by seed germination, contains the genotype of Parent, therefore, cannot inherit completely Parent merit; And Stem tip induction produces that rhizomatic tissue culture technique difficulty is comparatively large and the time is longer, very easily brownization is dead, even if not dead, induce generation root-like stock, also will reach half a year many, even year, applies very difficult in Chunlan proliferation.
Summary of the invention
For above-mentioned Problems existing, the invention provides the rhizomatic growing method of a kind of Chunlan, to overcome in prior art the problem that the root-like stock adopting seed germination to obtain cannot inherit the merit of Parent completely, also overcome in prior art and adopt Stem tip induction acquisition root-like stock cultivation difficulty large and the problem that the time is longer, thus ensure that the root-like stock grown can inherit the merit of Parent completely, and root-like stock can be grown fast, easily, be applicable to factory's industrialization and produce.
To achieve these goals, the technical scheme that the present invention takes is:
The rhizomatic growing method of a kind of Chunlan, wherein, comprising:
(1) get the raw materials ready: to prepare in one pelvic surface of sacrum without glaze and material is the Pot for cultivating plant of non-plastic, preparing a large amount of dry branches and fallen leaves humus soil is basin soil, prepares a robust growth and has the Chunlan sprouting sprouting ability;
(2) pretreatment: reject withered on described Chunlan, lose leaf, is placed under temperature is no more than the environment of 20 DEG C, until the root tissue water status of described Chunlan runs off 20% ~ 60% by described Chunlan;
(3) cultivate: described Chunlan is put into Pot for cultivating plant until the bulb of described Chunlan is directly touched at the bottom of the basin of described Pot for cultivating plant, and the coring of described Chunlan all directly contacts at the bottom of basin, then train into described basin soil on described Chunlan, wherein, the blade of described Chunlan exposes described basin soil surface at least 5cm;
(4) manage and protect: the described Chunlan cultivated is moved to cool place, steady wind place, and manage and protect 1.5 ~ 2.5 months under the condition of intensity of illumination 2000-3000lux, just can see on the bulb of described Chunlan has Chunlan root-like stock to grow.
The rhizomatic growing method of above-mentioned Chunlan, wherein, described Pot for cultivating plant is the one in high foot washing basin, shape sand basin and mud basin.
The rhizomatic growing method of above-mentioned Chunlan, wherein, the cylinder of described Pot for cultivating plant is dark is 20 ~ 30cm.
The rhizomatic growing method of above-mentioned Chunlan, wherein, the humus soil that described basin soil adopts dry branches and fallen leaves to land rotten 10 ~ 14 months.
The rhizomatic growing method of above-mentioned Chunlan, wherein, the described Chunlan in step (1) is the plant that 2-3 is raw, and at least 3 strain one clump.
The rhizomatic growing method of above-mentioned Chunlan, wherein, the thickness of the basin soil in Pot for cultivating plant described in step (3) is 10 ~ 20cm.
Technique scheme tool has the following advantages or beneficial effect:
The invention provides the rhizomatic growing method of Chunlan and adopt special planting technology, the lateral bud for Chunlan carries out induction plantation and cultivates, and select suitable planting conditions, make it develop into Chunlan root-like stock, the tissue cultures for Chunlan provides a new technology.Can simply obtain Chunlan root-like stock fast, the production system of setting up a factory Chunlan plantlet in vitro, for China's Chunlan industry provides a large amount of high-quality seedling, make up the deficiency in Chunlan market, meanwhile, new excellent special cymbidium varieties is cultivated, for Chinese Chunlan breeding provides a new way by Chunlan root-like stock.
The present invention utilizes Chunlan plant culture in blue basin, foster through 1.5 ~ 2.5 months transplant, thus obtains Chunlan root-like stock, and whole process does not exist the problem whether polluted; And existingly organize rapid propagation in vitro to obtain Chunlan root-like stock technology from the viewpoint of drawing materials and explant sterilization by Chunlan bud, due to Chunlan sprouting contact soil, be bacterial contamination comparatively serious, once sterilization is clean, just easily cause medium to pollute, explant death.Therefore, present invention, avoiding Chunlan sprouting as explant sterilization the problem such as difficult and operating difficulties, and material needed for the rhizomatic process of growth of Chunlan is less, and cost is lower.
On the other hand, from inductivity, the easy degree of survival rate and differentiation, utilize and existingly organize rapid propagation in vitro to obtain Chunlan root-like stock technology by Chunlan bud to carry out tissue cultures, explant Chunlan sprouting very easily brownization is dead, even if not dead, induce generation root-like stock, also half a year will be reached many, even year, and the rhizomatic growing method of Chunlan provided by the invention, simple and effective, on average every Chunlan can obtain a root-like stock through the cultivation of 1.5 ~ 2.5 months, length reaches more than 2cm, it is the explant raw material of very rare Chunlan tissue cultures, for Chunlan tissue cultures solves the large problem of raw material.
Therefore, the rhizomatic growing method of Chunlan provided by the invention overcomes in prior art the problem that the root-like stock adopting seed germination to obtain cannot inherit the merit of Parent completely, also overcome in prior art and adopt Stem tip induction acquisition root-like stock cultivation difficulty large and the problem that the time is longer, thus ensure that the root-like stock grown can inherit the merit of Parent completely, and root-like stock can be grown fast, easily, be applicable to factory's industrialization and produce.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated, but not as limiting to the invention.
Embodiment 1:
The rhizomatic growing method of the Chunlan that the embodiment of the present invention 1 provides comprises:
(1) get the raw materials ready: to prepare in one pelvic surface of sacrum without glaze and material is the Pot for cultivating plant of non-plastic, preparing a large amount of dry branches and fallen leaves humus soil is basin soil, prepares a robust growth and has the Chunlan sprouting sprouting ability;
(2) pretreatment: reject withered on Chunlan, lose leaf, Chunlan is placed on temperature be no more than the environment of 20 DEG C under (shady and cool place), 20% ~ 60% (now, the root system of Chunlan is soft) until the root tissue water status of Chunlan runs off;
(3) cultivate: Chunlan is put into Pot for cultivating plant until the bulb of Chunlan is directly touched at the bottom of the basin of Pot for cultivating plant, and the coring of Chunlan all directly contacts at the bottom of basin, then on Chunlan, trains engagement soil, wherein, the blade of Chunlan exposes basin soil surface at least 5cm;
(4) manage and protect: the Chunlan cultivated is moved to cool place, steady wind place, and 1.5 ~ 2.5 months are managed and protected under the condition of intensity of illumination 2000-3000lux, as 1.5 months, 2 months, 2.5 months etc., just can see on the bulb of Chunlan and have Chunlan root-like stock to grow.
In the rhizomatic growing method of Chunlan that the embodiment of the present invention 1 provides, Pot for cultivating plant is the one in high foot washing basin, shape sand basin and mud basin, and the cylinder of Pot for cultivating plant dark be 20 ~ 30cm, as 20cm, 22cm, 25cm, 28cm, 30cm etc., can not too deeply can not be too shallow, to ensure that Chunlan is cultivated after in Pot for cultivating plant, the blade of Chunlan exposes basin soil surface at least 5cm, and the thickness of basin soil in Pot for cultivating plant is 10 ~ 20cm, as 10cm, 12cm, 15cm, 18cm, 20cm etc.
In the rhizomatic growing method of Chunlan that the embodiment of the present invention 1 provides, the humus soil that basin soil adopts dry branches and fallen leaves to land rotten 10 ~ 14 months, as 10 months, 11 months, 12 months, 13 months, 14 months etc., humus soil is not now also completely Powdered, dry branches and fallen leaves also maintains original form, such basin soil is conducive to the eliminating of water, is conducive to the reservation of gas simultaneously, can meets the particular/special requirement of Chunlan to aqueous vapor completely.
In the rhizomatic growing method of Chunlan that the embodiment of the present invention 1 provides, described Chunlan in step (1) is the plant that 2-3 is raw, and at least 3 strain one clump (not separating), such Chunlan is more healthy and stronger and ability that is sprouting sprouting is better, thus ensure that the rhizomatic growth of Chunlan.
In sum, the invention provides the rhizomatic growing method of Chunlan and adopt special planting technology, the lateral bud for Chunlan carries out induction plantation and cultivates, and selects suitable planting conditions, make it develop into Chunlan root-like stock, the tissue cultures for Chunlan provides a new technology.Can simply obtain Chunlan root-like stock fast, the production system of setting up a factory Chunlan plantlet in vitro, for China's Chunlan industry provides a large amount of high-quality seedling, make up the deficiency in Chunlan market, meanwhile, new excellent special cymbidium varieties is cultivated, for Chinese Chunlan breeding provides a new way by Chunlan root-like stock.
The present invention utilizes Chunlan plant culture in blue basin, foster through 1.5 ~ 2.5 months transplant, thus obtains Chunlan root-like stock, and whole process does not exist the problem whether polluted; And existingly organize rapid propagation in vitro to obtain Chunlan root-like stock technology from the viewpoint of drawing materials and explant sterilization by Chunlan bud, due to Chunlan sprouting contact soil, be bacterial contamination comparatively serious, once sterilization is clean, just easily cause medium to pollute, explant death.Therefore, present invention, avoiding Chunlan sprouting as explant sterilization the problem such as difficult and operating difficulties, and material needed for the rhizomatic process of growth of Chunlan is less, and cost is lower.
On the other hand, from inductivity, the easy degree of survival rate and differentiation, utilize and existingly organize rapid propagation in vitro to obtain Chunlan root-like stock technology by Chunlan bud to carry out tissue cultures, explant Chunlan sprouting very easily brownization is dead, even if not dead, induce generation root-like stock, also half a year will be reached many, even year, and the rhizomatic growing method of Chunlan provided by the invention, simple and effective, on average every Chunlan can obtain a root-like stock through the cultivation of 1.5 ~ 2.5 months, length reaches more than 2cm, it is the explant raw material of very rare Chunlan tissue cultures, for Chunlan tissue cultures solves the large problem of raw material.
Therefore, the rhizomatic growing method of Chunlan provided by the invention overcomes in prior art the problem that the root-like stock adopting seed germination to obtain cannot inherit the merit of Parent completely, also overcome in prior art and adopt Stem tip induction acquisition root-like stock cultivation difficulty large and the problem that the time is longer, thus ensure that the root-like stock grown can inherit the merit of Parent completely, and root-like stock can be grown fast, easily, be applicable to factory's industrialization and produce.
It should be appreciated by those skilled in the art that those skilled in the art can realize described change case in conjunction with prior art and above-described embodiment, do not repeat them here.Such change case does not affect flesh and blood of the present invention, does not repeat them here.
Above preferred embodiment of the present invention is described.It is to be appreciated that the present invention is not limited to above-mentioned particular implementation; Any those of ordinary skill in the art, make many possible variations and modification not departing from technical solution of the present invention, or be revised as the Equivalent embodiments of equivalent variations, this does not affect flesh and blood of the present invention.Therefore, every content not departing from technical solution of the present invention, according to technical spirit of the present invention to any simple modification made for any of the above embodiments, equivalent variations and modification, all still belongs in the scope of technical solution of the present invention protection.
Claims (6)
1. the rhizomatic growing method of Chunlan, is characterized in that, comprising:
(1) get the raw materials ready: to prepare in one pelvic surface of sacrum without glaze and material is the Pot for cultivating plant of non-plastic, preparing a large amount of dry branches and fallen leaves humus soil is basin soil, prepares a robust growth and has the Chunlan sprouting sprouting ability;
(2) pretreatment: reject withered on described Chunlan, lose leaf, is placed under temperature is no more than the environment of 20 DEG C, until the root tissue water status of described Chunlan runs off 20% ~ 60% by described Chunlan;
(3) cultivate: described Chunlan is put into Pot for cultivating plant until the protocorm of described Chunlan is directly touched at the bottom of the basin of described Pot for cultivating plant, and the coring of described Chunlan all directly contacts at the bottom of basin, then train into described basin soil on described Chunlan, wherein, the blade of described Chunlan exposes described basin soil surface at least 5cm;
(4) manage and protect: the described Chunlan cultivated is moved to cool place, steady wind place, and manage and protect 1.5 ~ 2.5 months under the condition of intensity of illumination 2000-3000lux, just can see on the bulb of described Chunlan has Chunlan root-like stock to grow.
2. the rhizomatic growing method of Chunlan as claimed in claim 1, it is characterized in that, described Pot for cultivating plant is the one in high foot washing basin, shape sand basin and mud basin.
3. the rhizomatic growing method of Chunlan as claimed in claim 1 or 2, it is characterized in that, the cylinder of described Pot for cultivating plant is dark is 20 ~ 30cm.
4. the rhizomatic growing method of Chunlan as claimed in claim 1, is characterized in that, the humus soil that described basin soil adopts dry branches and fallen leaves to land rotten 10 ~ 14 months.
5. the rhizomatic growing method of Chunlan as claimed in claim 1, is characterized in that, the described Chunlan in step (1) is the plant that 2-3 is raw, and at least 3 strain one clump.
6. the rhizomatic growing method of Chunlan as claimed in claim 1, it is characterized in that, the thickness of the basin soil in Pot for cultivating plant described in step (3) is 10 ~ 20cm.
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| CN201510340452.9A CN104904482B (en) | 2015-06-18 | 2015-06-18 | The rhizomatic growing method of Chunlan |
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| CN201510340452.9A CN104904482B (en) | 2015-06-18 | 2015-06-18 | The rhizomatic growing method of Chunlan |
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| CN104904482A true CN104904482A (en) | 2015-09-16 |
| CN104904482B CN104904482B (en) | 2017-03-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109526711A (en) * | 2018-10-19 | 2019-03-29 | 杭州西湖风景名胜区（杭州市园林文物局）灵隐管理处（杭州花圃） | A kind of light green cloud rapid propagation method of Chunlan name |
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| CN101822220A (en) * | 2010-05-11 | 2010-09-08 | 浙江大学 | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii |
| CN103314862A (en) * | 2013-07-16 | 2013-09-25 | 葛建 | High-efficiency method for obtaining cymbidium detoxified seedling |
| CN103704130A (en) * | 2014-01-03 | 2014-04-09 | 江苏里下河地区农业科学研究所 | Chinese orchid and cymbidium hybridum hybrid seedling raising method |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101822220A (en) * | 2010-05-11 | 2010-09-08 | 浙江大学 | Method for culturing and rapidly propagating stem tip tissue of rare cymbidium goeringii |
| CN103314862A (en) * | 2013-07-16 | 2013-09-25 | 葛建 | High-efficiency method for obtaining cymbidium detoxified seedling |
| CN103704130A (en) * | 2014-01-03 | 2014-04-09 | 江苏里下河地区农业科学研究所 | Chinese orchid and cymbidium hybridum hybrid seedling raising method |
Non-Patent Citations (3)
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| 张菊野等: "几种影响春兰原球茎生长与分化的因素", 《植物生理学通讯》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109526711A (en) * | 2018-10-19 | 2019-03-29 | 杭州西湖风景名胜区（杭州市园林文物局）灵隐管理处（杭州花圃） | A kind of light green cloud rapid propagation method of Chunlan name |
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