CN104903914B - 用于定义细胞培养皿中感兴趣的对象周围的隔离区的方法 - Google Patents

用于定义细胞培养皿中感兴趣的对象周围的隔离区的方法 Download PDF

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Publication number
CN104903914B
CN104903914B CN201380069532.4A CN201380069532A CN104903914B CN 104903914 B CN104903914 B CN 104903914B CN 201380069532 A CN201380069532 A CN 201380069532A CN 104903914 B CN104903914 B CN 104903914B
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China
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image
colony
sample
culture dish
interested
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Expired - Fee Related
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CN201380069532.4A
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Chinese (zh)
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CN104903914A (zh
Inventor
劳伦·埃拉诺
纪尧姆·苏特拉
罗瑞德·穆诺兹
科琳·菲尔希龙
D·德科
弗莱德瑞克·皮恩斯顿
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Biomerieux SA
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Biomerieux SA
Commissariat à l'Énergie Atomique et Aux Énergies Alternatives
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    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/77—Retouching; Inpainting; Scratch removal
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/12—Edge-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/70—Determining position or orientation of objects or cameras
    • G06T7/73—Determining position or orientation of objects or cameras using feature-based methods
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/90—Determination of colour characteristics
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/693—Acquisition
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10016—Video; Image sequence
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10024—Color image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10152—Varying illumination
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20048—Transform domain processing
    • G06T2207/20061—Hough transform
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20076—Probabilistic image processing
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20112—Image segmentation details
    • G06T2207/20152—Watershed segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30242—Counting objects in image

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  • Engineering & Computer Science (AREA)
  • Theoretical Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Quality & Reliability (AREA)
  • Radiology & Medical Imaging (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medical Informatics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Image Analysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201380069532.4A 2012-11-07 2013-11-07 用于定义细胞培养皿中感兴趣的对象周围的隔离区的方法 Expired - Fee Related CN104903914B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12306372.9A EP2731051A1 (en) 2012-11-07 2012-11-07 Bio-imaging method
EP12306372.9 2012-11-07
PCT/EP2013/073291 WO2014072422A1 (en) 2012-11-07 2013-11-07 Bio-imaging method

Publications (2)

Publication Number Publication Date
CN104903914A CN104903914A (zh) 2015-09-09
CN104903914B true CN104903914B (zh) 2019-06-07

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US (1) US9576181B2 (cg-RX-API-DMAC7.html)
EP (2) EP2731051A1 (cg-RX-API-DMAC7.html)
JP (1) JP6062059B2 (cg-RX-API-DMAC7.html)
CN (1) CN104903914B (cg-RX-API-DMAC7.html)
AU (1) AU2013343537B2 (cg-RX-API-DMAC7.html)
WO (1) WO2014072422A1 (cg-RX-API-DMAC7.html)

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CN108102918B (zh) * 2018-01-03 2020-07-07 京东方科技集团股份有限公司 一种培养皿装置及细胞膜片的培养方法
WO2020021604A1 (ja) * 2018-07-23 2020-01-30 株式会社島津製作所 マイクロ流体デバイス観察装置及びマイクロ流体デバイス観察方法
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CN110992368A (zh) * 2019-11-08 2020-04-10 武汉互创联合科技有限公司 一种培养皿成像定位方法及装置
CN110724627B (zh) * 2019-11-15 2022-12-09 长春长光辰英生物科学仪器有限公司 单细胞分选仪的样品菌落定位装置与定位方法
CN111027527A (zh) * 2019-11-21 2020-04-17 四川极智朗润科技有限公司 一种基于光学频率特征的图像特征识别方法
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Also Published As

Publication number Publication date
JP2015536140A (ja) 2015-12-21
EP2731051A1 (en) 2014-05-14
WO2014072422A1 (en) 2014-05-15
CN104903914A (zh) 2015-09-09
US9576181B2 (en) 2017-02-21
JP6062059B2 (ja) 2017-01-18
EP2917873A1 (en) 2015-09-16
AU2013343537B2 (en) 2017-03-23
US20150278575A1 (en) 2015-10-01
AU2013343537A1 (en) 2015-05-21

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