CN104893991A - Microorganism composite preparation and application thereof - Google Patents
Microorganism composite preparation and application thereof Download PDFInfo
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- CN104893991A CN104893991A CN201510360012.XA CN201510360012A CN104893991A CN 104893991 A CN104893991 A CN 104893991A CN 201510360012 A CN201510360012 A CN 201510360012A CN 104893991 A CN104893991 A CN 104893991A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The invention relates to the field of microbial remediation, in particular to microorganism composite preparation and application thereof. The microorganism composite preparation mainly comprises arbuscular mycorrhizal fungi and Glomus versiforme. The invention further provides the application of the microorganism composite preparation in soil improving. The microorganism composite preparation has the advantages that the arbuscular mycorrhizal fungi is used together with the Glomus versiforme, the use effect of the microorganism composite preparation is evidently better than that of the independent arbuscular mycorrhizal fungus inoculant or Glomus versiforme inoculant, biomass increasing can be evidently promoted, soil structure can be improved, soil remediation can be achieved, heavy metal contents are lowered, and soil continuous cropping is overcome.
Description
Technical field
The present invention relates to microorganism remediation field, in particular to a kind of microbial compound preparation and application thereof.
Background technology
Along with the construction of ecotope, China closes down mining area ecological environment fragility, and on the spot envrionment conditions is poor, and rehabilitation cost is high, weak effect, and repairing effect is unstable, and even, the mining area of severe contamination also also exists the safety problem of harm mining area and nearby residents life and life.Current restoration of the ecosystem can adopt the solid soil of physical method, planting plants carries out restoration of the ecosystem.
But because mining area production region conditions is poor, some mining area does not even have soil or water resources very deficient.In standard biologic restorative procedure, Plants types choice is single, plantation surviving rate is low, some can not normal growth growth etc.
In view of this, special proposition the present invention.
Summary of the invention
The first object of the present invention is to provide a kind of microbial compound preparation, described microbial compound preparation sets up the symbiotic relationship of plant and mycorrhizal fungi, improve the symbiosis degree of plant and mycorhiza, refractory components in mycelia activating soil, promote Nutrient Absorption, increase the advantages such as phytomass.
The second object of the present invention is the application providing a kind of described microbial compound preparation.
In order to realize above-mentioned purpose of the present invention, spy by the following technical solutions:
A kind of microbial compound preparation, main containing bush mycorrhizal fungi and Glomus versiforme.
Microbial compound preparation provided by the invention, be by bush mycorrhizal fungi and Glomus versiforme with the use of, the microbial compound preparation result of use obtained is significantly better than the result of use being used alone bush mycorrhizal fungi preparation or Glomus versiforme microbial inoculum, there is significant promotion biomass increase, improvement Soil structure, rehabilitating soil, reduces heavy metal content, overcomes the effect of soil continuous cropping.
Bush mycorrhizal fungi preparation is the biotechnological formulation of common bush mycorrhizal fungi, and similarly, Glomus versiforme microbial inoculum is the biotechnological formulation of common Glomus versiforme.Bush mycorrhizal fungi preparation and Glomus versiforme microbial inoculum are prepared according to existing method or by commercially available purchase.Bush mycorrhizal fungi and Glomus versiforme common bacterial classification disclosed in prior art, can buy from DSMZ, also can be bought by other approach.
Preferably, described bush mycorrhizal fungi exists with the form of bush mycorrhizal fungi preparation, and described Glomus versiforme exists with the form of Glomus versiforme microbial inoculum.
In order to strengthen the mutual chemiluminescence between bush mycorrhizal fungi and Glomus versiforme, better to strengthen the effect that bush mycorrhizal fungi and Glomus versiforme work in coordination with enhancing, preferably, by weight, described bush mycorrhizal fungi preparation 3-8 part, described Glomus versiforme microbial inoculum 1-5 part.
More preferably, by weight, described bush mycorrhizal fungi preparation 5-7 part, described Glomus versiforme microbial inoculum 2-3 part.
Further, described bush mycorrhizal fungi preparation is prepared by the following method:
Take corn as host, by weight, husky for after matrix sterilizing with 0.8-1.5 part peat composed of rotten mosses and 0.8-1.5 part, Arbuscular Mycorrhizal Fungi, expand numerous 55-65 days, the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated are described bush mycorrhizal fungi preparation.Wherein, the inoculum size of bush mycorrhizal fungi is the 8%-15% of matrix weight.
Further, described Glomus versiforme microbial inoculum is prepared by the following method:
Take corn as host, by weight, husky for after matrix sterilizing with 0.8-1.5 part peat composed of rotten mosses and 0.8-1.5 part, inoculation Glomus versiforme, expand numerous 55-65 days, the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated are described Glomus versiforme microbial inoculum.Wherein, the inoculum size of Glomus versiforme is the 8%-15% of matrix weight.
Be that the consistency that host expands between numerous bush mycorrhizal fungi preparation of obtaining and Glomus versiforme microbial inoculum bacterium is good with corn, thalli growth is vigorous, is easy to symplastic growth, and collaborative strengthen between mutual effect.
In order to microbial compound preparation reaches better effect, and be easy to preservation, preferably, described microbial compound preparation also comprises auxiliary material.
Particularly, by weight percentage, described auxiliary material is made primarily of following raw material:
Acrylamide 60%-70%, potassium acrylate 20%-25%, water 5%-10%, linking agent 0.4%-0.7%.
More preferably, by weight percentage, described auxiliary material is made primarily of following raw material:
Acrylamide 65%, potassium acrylate 23%, water 8%, linking agent 0.5%.
Further, by weight, described auxiliary material is 0.5-1.5 part.
Present invention also offers described microbial compound preparation and improve the application in soil.
Preferably, described microbial compound preparation and seed with the use of, the usage quantity of described microbial compound preparation is the 20%-30% of seed weight.
Microbial compound preparation mainly contains following use-pattern:
(1) seed disinfection: with seed potassium permanganate 2000-3000 times of liquid disinfectant 5 minutes of sowing, need prepare with cold water;
Inoculating active microbial inoculum: at seeding time, administered formulation in seed furrow or cave, usage quantity is the 20-30% of seed weight, puts seed thereon after first putting microbial compound preparation, afterwards by normally after planting managing.
(2) preparation seed dressing uses: seed and microbial compound preparation are put into clean water according to 10:2 ratio, and consumption is to form scattered paste shape for appropriateness, and seed-coat forms adhesion and is advisable, and normally sows management afterwards.
The symbiotic plant of microbial compound preparation provided by the invention comprises:
Most of gardening plants such as the Rosaceae except Cruciferae, Chenopodiaceae, Amaranthaceae, JUNCACEAE, the orchid family, Pinaceae, Cupressaceae, Fagaceae, Yang Ke, corylaceae, Gramineae, Curcurbitaceae, Solanaceae.
Preferably, described microbial compound preparation with except Cruciferae, Chenopodiaceae, Amaranthaceae, JUNCACEAE, the orchid family, Pinaceae, Cupressaceae, Fagaceae, Yang Ke, corylaceae gardening plant with the use of.
Compared with prior art, beneficial effect of the present invention is:
(1) Promoting plant growth, increases phosphorus and absorbs
The mining area of condition difference is stood on colliery and other places, utilize microbial preparation Combination application in different plants, the typical mycorhiza of root growth, mycelia expands the absorption area of root system, strengthen root system to the utilising efficiency of soil insoluble nutrition, promote that phytomass increases, improve the effect that mining-area plant is repaired.
(2) some heavy metal content of soil is reduced, improvement Soil structure
The microbial compound preparation that the application of the invention provides, promote that root system is to the absorption of heavy metal in soil, in mining soil, the heavy metal such as cadmium, lead has certain reduction; The formation of mycorhiza further improves the structure of rhizosphere soil, and porosity and water content etc. increase.
(3) continuous cropping is overcome
By the Seeding planting of microbe inoculation preparation in continuous cropping soil, can grow by normal growth.
(4) set up the symbiotic relationship of plant and mycorrhizal fungi, improve the symbiosis degree of plant and mycorhiza, refractory components in mycelia activating soil, promote Nutrient Absorption, increase phytomass.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below.
Fig. 1 is wheatgrass mycorhiza figure (100 ×) in the embodiment of the present invention 4;
Fig. 2 is wheatgrass mycorhiza figure (400 ×) in the embodiment of the present invention 4;
Fig. 3 is apricot mycorhiza figure (100 ×) in the embodiment of the present invention 4;
Fig. 4 is apricot mycorhiza figure (400 ×) in the embodiment of the present invention 4;
Fig. 5 is the strawberry mycorhiza figure using microbial compound preparation provided by the invention in the embodiment of the present invention 4;
Fig. 6 is the strawberry mycorhiza figure not using microbial compound preparation provided by the invention in the embodiment of the present invention 4.
Embodiment
Below in conjunction with embodiment, embodiment of the present invention are described in detail, but it will be understood to those of skill in the art that the following example only for illustration of the present invention, and should not be considered as limiting the scope of the invention.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturers suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, being can by the conventional products of commercially available acquisition.
Embodiment 1
Take corn as host, husky for after matrix sterilizing with the 1kg peat composed of rotten mosses and 1kg, Arbuscular Mycorrhizal Fungi, the inoculum size of bush mycorrhizal fungi is 10% of matrix weight, namely the inoculum size of bush mycorrhizal fungi is 0.2kg, expand numerous 60 days, namely the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated obtain bush mycorrhizal fungi preparation, for subsequent use;
Take corn as host, husky for after matrix sterilizing with the 1kg peat composed of rotten mosses and 1kg, inoculation Glomus versiforme, the inoculum size of Glomus versiforme is 10% of matrix weight, namely the inoculum size of Glomus versiforme is 0.2kg, expand numerous 60 days, namely the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated obtain Glomus versiforme microbial inoculum, for subsequent use;
Auxiliary material is made, acrylamide 65%, potassium acrylate 23%, water 7%, linking agent 0.5% by following weight percent, for subsequent use;
Get bush mycorrhizal fungi preparation 5kg, Glomus versiforme microbial inoculum 3kg, auxiliary material 1kg, mix and obtain microbial compound preparation;
Grass seed is sterilized: with seed potassium permanganate 2000 times of liquid disinfectants 5 minutes of sowing, need prepare with cold water;
Microbe inoculation compound formulation: in mining area sowing, first use microbial compound preparation in seed furrow or cave, usage quantity is 20% of seed weight, then is put on microbial compound preparation by seed, afterwards by normally after planting managing.
Meanwhile, not inoculate any microbial inoculum as a control group 1; With independent Arbuscular Mycorrhizal Fungi microbial inoculum as a control group 2; To inoculate separately Glomus versiforme microbial inoculum as a control group 3.
1. improve Soil structure
Microbial inoculum, after mixed with seed 2 months, gets the soil test soil weight, soil porosity, water content at random.Concrete measuring method is:
1.1 soil basis determinings adopt alcohol combustion method: claim furnace pot weight (A) with 1/100 balance; Getting for surveying soil sample 8-10g, putting into rapidly the furnace pot that oneself knows weight, weighing (B) with 1/100 balance; In furnace pot, drip alcohol with dropper, until the whole submergence in native face, vibrate a little, make soil sample be uniformly distributed in ware; Light alcohol with match, until fray-out of flame sample cooling after, then drip 1.5-2ml alcohol carry out second time burn; Weigh (C) after soil sample cooling, be accurate to 0.01g;
In formula: A-furnace pot heavy (g);
B-wet soil heavily adds furnace pot heavy (g);
C-dry ground heavily adds furnace pot heavy (g).
1.2 soil weight measure and adopt core cutter method: the soil sample utilizing the steel cutting ring cutting natural situation of certain volume, makes it be full of wherein, then weigh the oven-dried soil weight calculating unit volume.Get clean cutting ring (not being with top iron ring) to put on 1/10 balance and weigh (A), and accurately calculate the volume (B) of cutting ring; Cutting ring top puts iron ring, then blade one end is pressed into gently for surveying in soil layer, soil is filled and reaches iron ring upper limb, then carefully soil sample barrel is dug out with skin knife, be inverted on little plank, with skin knife, soil around cutting ring cut only, and blade one end is scabbled, build with little plank, finally make soil volume identical with soil sample barrel volume; Cutting ring with soil sample is placed on 1/10 balance and weighs (C), then in cutting ring, take out about 10 soil mensuration moisture contents, be converted into oven-dried soil weight;
In formula: A-cutting ring heavy (g);
B-cutting ring volume (cm3);
C-cutting ring heavily adds wet soil heavy (g) in cutting ring.
1.3 soil porosities measure:
Soil porosity size is directly connected to ventilation and the water regime of soil, is one of Main physical character of soil.Soil porosity does not generally directly measure, but is tried to achieve by specific gravity of soil and unit weight indirect calculation.Specific gravity of soil be the soil weight of unit volume and 4 DEG C time consubstantiality ponding weight ratio.
Measure Soil structure the results are shown in Table 1.
Table 1 different treatment is on the impact of Soil structure
As can be seen from Table 1, after microbial compound preparation process soil provided by the invention, soil porosity increases, and the soil weight declines, and water content is moderate.
2. mining area sward test: first batch of grass was gathered in the crops in early July, different treatment mycorhiza forms the biomass careless with first batch in table 2.
Table 2 different treatment mycorhiza forms the biomass careless with first batch
As can be seen from Table 2, use the mining area after microbial compound preparation process provided by the invention, the mycorhiza rate of grass has had and has very significantly improved, and fresh biomass there has also been and significantly improves.
At later stages (August), the increment of herbage is large, inoculate the average 6.08g/ strain of eldish of microbial compound preparation provided by the invention, the contrast not inoculating microbial inoculum is 1.41g/ strain, and the contrast of independent Arbuscular Mycorrhizal Fungi microbial inoculum is 3.1g/ strain; The contrast of independent inoculation Glomus versiforme microbial inoculum is 3.4g/ strain.Calculate according to density, Weight per plant and its water content, cut 3 times every year, use the total grass yield of microbial compound preparation process provided by the invention can reach 1200-1600kg/ mu, the contrast not inoculating microbial inoculum is 500-1100kg/ mu, and the contrast of independent Arbuscular Mycorrhizal Fungi microbial inoculum is 700-750kg/ mu; The contrast of independent inoculation Glomus versiforme microbial inoculum is 720-780kg/ mu.
3. rehabilitating soil, reduces heavy metal content
Cadmium in Soil, plumbous measuring method: adopt Inductively coupled plasma optical emission spectrometer to measure the content of heavy metal in soil Cd (cadmium) and Pb (lead), take the testing soil 1g of microbial inoculum after mixed with seed 2 months in alimentary canal, add the 10ml vitriol oil, 160 DEG C are spent the night.260 DEG C of digestion 1h, after 320 DEG C of digestion 1h, add 4,5, hydrogen peroxide, and 320 DEG C of heating 1h, add hydrogen peroxide 1ml.Until be residue in alimentary canal after repeating 5-6 time.Solution deionized water is settled to 100ml and is used for element determination.
The result obtained is as shown in table 3.
Table 3 different treatment heavy metal in soil content
| Process | Cd(mg/kg) | Pb(mg/kg) |
| Control group 1 | 0.097 | 11.1 |
| Control group 2 | 0.097 | 9.4 |
| Control group 3 | 0.107 | 11.6 |
| Microbial compound preparation | 0.091 | 10.7 |
As can be seen from Table 3, the heavy metal in soil Cd after applying microbial compound preparation provided by the invention and Pb content decline all to some extent.
Embodiment 2
Take corn as host, husky for after matrix sterilizing with the 0.8kg peat composed of rotten mosses and 1.5kg, Arbuscular Mycorrhizal Fungi, the inoculum size of bush mycorrhizal fungi is 15% of matrix weight, namely the inoculum size of bush mycorrhizal fungi is 0.345kg, expand numerous 55 days, namely the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated obtain bush mycorrhizal fungi preparation, for subsequent use;
Take corn as host, husky for after matrix sterilizing with the 0.8kg peat composed of rotten mosses and 1.5kg, inoculation Glomus versiforme, the inoculum size of Glomus versiforme is 15% of matrix weight, namely the inoculum size of Glomus versiforme is 0.345kg, expand numerous 55 days, namely the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated obtain Glomus versiforme microbial inoculum, for subsequent use;
Auxiliary material is made, acrylamide 60%, potassium acrylate 20%, water 5%, linking agent 0.4% by following weight percent, for subsequent use;
Get bush mycorrhizal fungi preparation 3kg, Glomus versiforme microbial inoculum 5kg, auxiliary material 0.5kg, mix and obtain microbial compound preparation;
Grass seed is sterilized: with seed potassium permanganate 3000 times of liquid disinfectants 5 minutes of sowing, need prepare with cold water;
Microbe inoculation compound formulation: in mining area sowing, first use microbial compound preparation in seed furrow or cave, usage quantity is 30% of seed weight, then is put on microbial compound preparation by seed, afterwards by normally after planting managing.
Meanwhile, not inoculate any microbial inoculum as a control group 1; With independent Arbuscular Mycorrhizal Fungi microbial inoculum as a control group 2; To inoculate separately Glomus versiforme microbial inoculum as a control group 3.
1. improve Soil structure
Measuring method is with embodiment 1, and result is consistent with embodiment 1.
2. mining area sward test
Measuring method is with embodiment 1, and result is consistent with embodiment 1.
3. rehabilitating soil, reduces heavy metal content
Measuring method is with embodiment 1, and result is consistent with embodiment 1.
Embodiment 3
Take corn as host, husky for after matrix sterilizing with the 1.5kg peat composed of rotten mosses and 0.8kg, Arbuscular Mycorrhizal Fungi, the inoculum size of bush mycorrhizal fungi is 8% of matrix weight, namely the inoculum size of bush mycorrhizal fungi is 0.184kg, expand numerous 65 days, namely the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated obtain bush mycorrhizal fungi preparation, for subsequent use;
Take corn as host, husky for after matrix sterilizing with the 1.5kg peat composed of rotten mosses and 0.8kg, inoculation Glomus versiforme, the inoculum size of Glomus versiforme is 8% of matrix weight, namely the inoculum size of Glomus versiforme is 0.184kg, expand numerous 65 days, namely the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated obtain Glomus versiforme microbial inoculum, for subsequent use;
Auxiliary material is made, acrylamide 70%, potassium acrylate 25%, water 10%, linking agent 0.7% by following weight percent, for subsequent use;
Get bush mycorrhizal fungi preparation 8kg, Glomus versiforme microbial inoculum 1kg, auxiliary material 1.5kg, mix and obtain microbial compound preparation;
Cucumber seeds and microbial compound preparation are put into clean water according to 10:2 ratio, and consumption is to form scattered paste shape for appropriateness, and seed-coat forms adhesion and is advisable, and is seeded in the soil of continuous cropping afterwards, normal management.
Meanwhile, not inoculate any microbial inoculum as a control group 1; With independent Arbuscular Mycorrhizal Fungi microbial inoculum as a control group 2; To inoculate separately Glomus versiforme microbial inoculum as a control group 3.
The cucumber index of plantation is measured, specifically as shown in table 4.
After table 4 microbe inoculation compound formulation, cucumber seedling is in continuous cropping soil increment
As can be seen from Table 4, microbial compound preparation provided by the invention is planted in the soil of continuous cropping, utilizes the seedling ground biomass of microbial compound preparation apparently higher than other groups after field planting.
Embodiment 4
Take corn as host, husky for after matrix sterilizing with the 1kg peat composed of rotten mosses and 1.2kg, Arbuscular Mycorrhizal Fungi, the inoculum size of bush mycorrhizal fungi is 10% of matrix weight, namely the inoculum size of bush mycorrhizal fungi is 0.22kg, expand numerous 60 days, namely the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated obtain bush mycorrhizal fungi preparation, for subsequent use;
Take corn as host, husky for after matrix sterilizing with the 1kg peat composed of rotten mosses and 1.2kg, inoculation Glomus versiforme, the inoculum size of Glomus versiforme is 15% of matrix weight, namely the inoculum size of Glomus versiforme is 0.33kg, expand numerous 60 days, namely the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated obtain Glomus versiforme microbial inoculum, for subsequent use;
Auxiliary material is made, acrylamide 65%, potassium acrylate 25%, water 8%, linking agent 0.5% by following weight percent, for subsequent use;
Get bush mycorrhizal fungi preparation 5kg, Glomus versiforme microbial inoculum 2kg, auxiliary material 1kg, mix and obtain microbial compound preparation;
Group 1: Agropyron seeds and microbial compound preparation are put into clean water according to 10:2 ratio, consumption is to form scattered paste shape for appropriateness, and seed-coat forms adhesion and is advisable, and sows afterwards, normal management;
Group 2: apricot seeds and microbial compound preparation are put into clean water according to 10:2 ratio, consumption is to form scattered paste shape for appropriateness, and seed-coat forms adhesion and is advisable, and sows afterwards, normal management;
Group 3: strawberry seed and microbial compound preparation are put into clean water according to 10:2 ratio, consumption is to form scattered paste shape for appropriateness, and seed-coat forms adhesion and is advisable, and sows afterwards, normal management.
Observe the mycorhiza of wheatgrass, apricot and strawberry respectively, mycorhiza dyeing adopts TB (the bent sharp benzene of Trypan Blue is blue) dyeing process.The concrete operations of TB dyeing process are: plant root segment is put into bottle, add 10% (w/v) KOH, in 90 DEG C of digestion 20 minutes, outwell KOH, again with water cleaning, with in 2% (v/v) HCl with 15 minutes, outwell HCl, add 0.05%TB dyestuff, in 90 DEG C of dyeing 10 minutes.After being cleaned with clear water by the root segment of dyeing, be cut into the size of 0.5-1.0cm, in dissecting Microscopic observation and selecting infection root segment.
Wherein, wheatgrass mycorhiza figure is as shown in Fig. 1 (100 ×) and Fig. 2 (400 ×);
Apricot mycorhiza figure is as shown in Fig. 3 (100 ×) and Fig. 4 (400 ×);
Strawberry mycorhiza figure is as shown in Fig. 5 (100 ×) and Fig. 6 (100 ×), and Fig. 5 is the mycorhiza using microbial compound preparation provided by the invention, and Fig. 6 is the mycorhiza not using microbial compound preparation provided by the invention.
As can be seen from Fig. 1 and Fig. 2, wheatgrass mycorhiza has VA spore and mycelia; As can be seen from Fig. 3 and Fig. 4, apricot mycorhiza has VA mycorhiza; As can be seen from Figure 5, the VA mycorhiza that strawberry is formed, does not use microbial compound preparation provided by the invention and does not then form VA mycorhiza.
VA mycorhiza energy Promoting plant growth, its mechanism is that mycelia expands the nutrition absorption space of root system, promote that plant is to the absorption of mineral nutrient, especially to the absorption of phosphorus, VA mycorhiza promotes that inhaling phosphorus is due to the insoluble inorganic phosphate in mycorrhizal fungi activating soil, organophosphorus and the mutual work with phosphate-solubilizing bacteria.Illustrate that microbial compound preparation provided by the invention has the symbiotic relationship setting up plant and mycorrhizal fungi, improve the symbiosis degree of plant and mycorhiza, refractory components in mycelia activating soil, promote Nutrient Absorption, increase the effect of phytomass.
Although illustrate and describe the present invention with specific embodiment, however it will be appreciated that can to make when not deviating from the spirit and scope of the present invention many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged in the scope of the invention in the following claims.
Claims (10)
1. a microbial compound preparation, is characterized in that, main containing bush mycorrhizal fungi and Glomus versiforme.
2. microbial compound preparation according to claim 1, is characterized in that, described bush mycorrhizal fungi exists with the form of bush mycorrhizal fungi preparation, and described Glomus versiforme exists with the form of Glomus versiforme microbial inoculum;
By weight, described bush mycorrhizal fungi preparation 3-8 part, described Glomus versiforme microbial inoculum 1-5 part.
3. microbial compound preparation according to claim 2, is characterized in that, described bush mycorrhizal fungi preparation is prepared by the following method:
Take corn as host, by weight, husky for after matrix sterilizing with 0.8-1.5 part peat composed of rotten mosses and 0.8-1.5 part, Arbuscular Mycorrhizal Fungi, the inoculum size of described bush mycorrhizal fungi is the 8%-15% of matrix weight, expand numerous 55-65 days, the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated are described bush mycorrhizal fungi preparation.
4. microbial compound preparation according to claim 2, is characterized in that, described Glomus versiforme microbial inoculum is prepared by the following method:
Take corn as host, by weight, husky for after matrix sterilizing with 0.8-1.5 part peat composed of rotten mosses and 0.8-1.5 part, inoculation Glomus versiforme, the inoculum size of described Glomus versiforme is the 8%-15% of matrix weight, expand numerous 55-65 days, the spore wherein containing fungi obtained, mycelia and the combined inoculation agent by the root segment contaminated are described Glomus versiforme microbial inoculum.
5. microbial compound preparation according to claim 1, is characterized in that, described microbial compound preparation also comprises auxiliary material.
6. microbial compound preparation according to claim 5, is characterized in that, by weight percentage, described auxiliary material is made primarily of following raw material:
Acrylamide 60%-70%, potassium acrylate 20%-25%, water 5%-10%, linking agent 0.4%-0.7%.
7. microbial compound preparation according to claim 6, is characterized in that, by weight, described auxiliary material is 0.5-1.5 part.
8. the microbial compound preparation described in any one of claim 1-7 is improving the application in soil.
9. application according to claim 8, is characterized in that, described microbial compound preparation and seed with the use of, the usage quantity of described microbial compound preparation is the 20%-30% of seed weight.
10. application according to claim 8, is characterized in that, described microbial compound preparation with except Cruciferae, Chenopodiaceae, Amaranthaceae, JUNCACEAE, the orchid family, Pinaceae, Cupressaceae, Fagaceae, Yang Ke, corylaceae gardening plant with the use of.
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| CN105505796A (en) * | 2016-01-08 | 2016-04-20 | 华南师范大学 | Method for reducing honeysuckle cadmium accumulation and increasing honeysuckle yield through glomus versiforme berch |
| CN110437002A (en) * | 2019-08-22 | 2019-11-12 | 四川大学 | Modifying agent increase soil fertility and preparation method thereof |
| CN110467924A (en) * | 2019-09-09 | 2019-11-19 | 山东胜伟盐碱地科技有限公司 | A kind of alkaline land soil mercury, cadmium ion remover and preparation method thereof |
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| CN112779177A (en) * | 2019-11-07 | 2021-05-11 | 中国科学院烟台海岸带研究所 | Compound microbial agent special for saline-alkali soil and application thereof |
| CN114164151A (en) * | 2021-12-03 | 2022-03-11 | 承德宝通矿业有限公司 | Phosphate-solubilizing complex microbial inoculant and application thereof |
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