CN104892777B - A kind of method for extraction and purification of lotus seeds amylose - Google Patents
A kind of method for extraction and purification of lotus seeds amylose Download PDFInfo
- Publication number
- CN104892777B CN104892777B CN201510263729.2A CN201510263729A CN104892777B CN 104892777 B CN104892777 B CN 104892777B CN 201510263729 A CN201510263729 A CN 201510263729A CN 104892777 B CN104892777 B CN 104892777B
- Authority
- CN
- China
- Prior art keywords
- sediment
- amylose
- lotus seeds
- purification
- extraction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 240000002853 Nelumbo nucifera Species 0.000 title claims abstract description 163
- 235000006508 Nelumbo nucifera Nutrition 0.000 title claims abstract description 163
- 235000006510 Nelumbo pentapetala Nutrition 0.000 title claims abstract description 163
- 229920000856 Amylose Polymers 0.000 title claims abstract description 114
- 238000000605 extraction Methods 0.000 title claims abstract description 70
- 238000000746 purification Methods 0.000 title claims abstract description 70
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 108
- 229920002472 Starch Polymers 0.000 claims abstract description 49
- 235000019698 starch Nutrition 0.000 claims abstract description 49
- 239000008107 starch Substances 0.000 claims abstract description 49
- 238000005119 centrifugation Methods 0.000 claims abstract description 36
- 239000000243 solution Substances 0.000 claims description 127
- 239000013049 sediment Substances 0.000 claims description 126
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 91
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 60
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 claims description 44
- 238000003756 stirring Methods 0.000 claims description 43
- 238000010438 heat treatment Methods 0.000 claims description 38
- 238000006243 chemical reaction Methods 0.000 claims description 36
- 238000009835 boiling Methods 0.000 claims description 30
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 28
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 23
- 239000007864 aqueous solution Substances 0.000 claims description 22
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- 238000004108 freeze drying Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 239000002244 precipitate Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 10
- 238000002360 preparation method Methods 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000004904 shortening Methods 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 38
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 11
- 229910052740 iodine Inorganic materials 0.000 description 11
- 239000011630 iodine Substances 0.000 description 11
- 235000019441 ethanol Nutrition 0.000 description 8
- 238000001816 cooling Methods 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- JLKDVMWYMMLWTI-UHFFFAOYSA-M potassium iodate Chemical compound [K+].[O-]I(=O)=O JLKDVMWYMMLWTI-UHFFFAOYSA-M 0.000 description 4
- 239000001230 potassium iodate Substances 0.000 description 4
- 235000006666 potassium iodate Nutrition 0.000 description 4
- 229940093930 potassium iodate Drugs 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 229920000945 Amylopectin Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000010411 cooking Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- DKNPRRRKHAEUMW-UHFFFAOYSA-N Iodine aqueous Chemical compound [K+].I[I-]I DKNPRRRKHAEUMW-UHFFFAOYSA-N 0.000 description 1
- 241000209447 Nelumbo Species 0.000 description 1
- 235000005807 Nelumbo Nutrition 0.000 description 1
- 244000213382 Nymphaea lotus Species 0.000 description 1
- 235000010710 Nymphaea lotus Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 229940075397 calomel Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical compound Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 150000004694 iodide salts Chemical class 0.000 description 1
- 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Substances [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to biochemical field, and in particular to the method for extraction and purification of lotus seeds amylose.Complicated, less efficient, long preparation period to be solved by this invention the step of be the method for extraction and purification of existing lotus seeds amylose, be difficult the technical problem accomplished scale production, thus propose that a kind of technique is simple, easily accomplish scale production, the method for extraction and purification for the lotus seeds amylose that yield height, purity are high.The method for extraction and purification of lotus seeds amylose of the present invention; using the lotus seed starch obtained by the preparation of dry lotus seeds as raw material; concentration by optimizing centrifugation rate and NaOH solution etc.; on the premise of lotus seeds amylose molecule structure is not destroyed; improve efficiency, the large-scale production for shortening the operating time, being conducive to lotus seeds amylose; and the obtained lotus seeds amylose purity of institute's extraction purification is high (about 94%), can as lotus seeds amylose content determination method standard.
Description
Technical field
The invention belongs to biochemical field, and in particular to a kind of method for extraction and purification of lotus seeds amylose.
Background technology
Lotus seeds are the fruit and the general name of seed of Nymphaeceae Nelumbo (Nelum bonucifera Gaertn) plant, mainly
The ground such as Jiangxi, Fujian, Hunan, Hubei are distributed in, are the important local product resources of China, with abundant nutritive and health protection components and medicine
Reason activity.According to《Compendium of Materia Medica》Record, there is lotus seeds bowl spares to repose, quench the thirst reduce phlegm and internal heat, stop dysentery of feeling at ease, kidney solid essence of laying one's heart bear,
The effects such as strengthening the bones and muscles, qi-restoratives damage, sharp knowledge, eliminating cold-damp and woman are with collapsing.
Starch is the main component of lotus seeds, accounts for more than the 50% of its dry.It is the same with the starch of most plants, lotus seeds
Starch is also to be made up of amylose and amylopectin.The lotus seeds that kind is different, source is different, its amylose content difference compared with
Greatly.Lotus seeds amylose content be influence the dissolubility of solid matter in lotus seeds water imbibitions, dilatancy, cooking, color, gloss,
The principal element of viscosity and flexibility, is also the key factor for determining Cooking Quality.
The molecular structure of lotus seeds amylose is the internal factor for determining its quality and quality, and molecular structure is different, its product
Matter and quality are also different.It is also influence and method for extraction and purification is the key factor for the molecular structure for influenceing lotus seeds amylose
The key factor of its quality and quality.On the premise of lotus seeds amylose molecule structure is not destroyed, lotus seeds amylose is studied
Method for extraction and purification, not only contribute to study lotus seed starch composition, and be conducive to research lotus seeds amylose content with
Corresponding relation between lotus seeds quality, so that related to lotus seeds quality for the seed selection of Lotus Seed Varieties, the exploitation of lotus seeds resource
The formulation of standard is provided fundamental basis.
Master thesis《The separation and its quality characteristic research of six kinds of lotus seed starch》(Tao Jinhong, Nanjing Normal University,
2009) a kind of method for extraction and purification of lotus seeds amylose is disclosed, i.e.,：Weigh 10g starch and cross 40 mesh sieves, be put into 500mL burnings
In cup, plus a small amount of absolute ethyl alcohol and distilled water, sample is moistened, is added in 0.5mol/L NaOH 350mL, boiling water bath is added
20~30min of thermal agitation centrifuges (4000r/min, 20min) to being completely dispersed after cooling, goes precipitation, then adjust with 2mol/L HCl
To neutrality.Add 100mL n-butanols and isoamyl alcohol (volume ratio 3:1) mixed liquor, then heating stirring 10min is extremely in boiling water bath
Solution is transparent, is cooled to room temperature, moves into after 4 DEG C of refrigerators stand 24 and centrifuges (4000r/min, 20min), obtained precipitation is thick
Amylose (Am), supernatant is thick amylopectin solution (Ap).200mL n-butanol saturated aqueous solutions are added in precipitation, then
Heating stirring is cooled to room temperature to solution dispersed transparent in boiling water bath, in 4 DEG C of refrigerator 24h, centrifuge (4000r/min,
20min), precipitated, repeated after aforesaid operations 5 times, the sediment obtained by collecting is dipped in 24h in absolute ethyl alcohol, and uses nothing
Water-ethanol is washed for several times, is dried at 40 DEG C, is produced amylose (Am) sample.
However, in actual production process, complicated, the efficiency the step of method for extraction and purification of above-mentioned lotus seeds amylose
Relatively low, long preparation period is difficult to realize large-scale production.
Therefore, on the premise of lotus seeds amylose molecule structure is not destroyed, how research improves lotus seeds amylose
The efficiency of method for extraction and purification, shortens the process time, has very important meaning for the large-scale production of lotus seeds amylose
Justice.
The content of the invention
Therefore, complicated the step of be the method for extraction and purification of existing lotus seeds amylose, effect to be solved by this invention
Rate is relatively low, long preparation period, the technical problem accomplished scale production is difficult, so as to propose that technique is simple, easily realize scale
Production, a kind of method for extraction and purification for lotus seeds amylose that yield is high, purity is high.
In order to solve the above technical problems, the present invention is achieved through the following technical solutions：
A kind of method for extraction and purification of lotus seeds amylose of the present invention, comprises the following steps：
(1) parts by weight of lotus seed starch 10 are weighed, the sodium hydroxide for adding 0.30-0.40 parts by volume 0.5-0.8mol/L is molten
Liquid, heating stirring 15-25min, to being completely dispersed, is cooled to room temperature；
(2) reaction solution for preparing the step (1) is centrifuged, and centrifugal speed is 4000-18000r/min, during centrifugation
Between be 15-25min, take supernatant, it is 6.0-7.0 to be adjusted to pH with 1.0-2.0mol/L hydrochloric acid；
(3) the mixed liquor 0.06-0.10 volumes of n-butanol/isoamyl alcohol are added in the reaction solution prepared to the step (2)
Part, heating stirring 10-20min is cooled to room temperature, and upper solution is discarded, lower sediment is carried out by 2-4 DEG C of standing 20-30h
Centrifugation, centrifugal speed is 4000-18000r/min, and centrifugation time is 5-15min, obtains sediment A；
(4) the sediment A is washed with saturation n-butanol aqueous solution, centrifuged, centrifugal speed is 4000-18000r/
Min, centrifugation time is 5-15min, obtains sediment B；
(5) sediment B is placed in saturation n-butanol aqueous solution, heating stirring 10-20min is cooled to room temperature, 2-
4 DEG C of standing 20-30h, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 4000-18000r/min, from
The heart time is 5-15min, obtains sediment C；
(6) the sediment C is placed in saturation n-butanol aqueous solution, heating stirring, purifies 4-6 times, must precipitate repeatedly
Thing D；
(7) sediment D is washed 2-5 times with ethanol, dries, produce lotus seeds amylose；
The relation of the parts by weight and parts by volume is g/L.
In the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention, the step (2), step (3), step (4) and
In at least one step of step (5), the centrifugal speed is 10000-18000r/min.
In the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention, in the step (1), the sodium hydroxide solution
Concentration be 0.6mol/L or 0.7mol/L.
In the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention, the step (1), step (3), step (5) and
It is described to be heated to be boiling water bath heating in step (6).
It is described quiet in the step (3) and step (5) in the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention
The time put is 24h.
In the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention, in the step (3), the n-butanol/isoamyl
The volume ratio of n-butanol and isoamyl alcohol is 1 in the mixed liquor of alcohol:1, mixed liquor and the step of the n-butanol/isoamyl alcohol
(2) volume ratio of the reaction solution prepared is 1：5.25-6.125.
It is described dry using freezing in the step (7) in the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention
Seasoning or boulton process.
In the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention, in the step (1), the lotus seed starch passes through
Following steps are made：
(a) after lotus seeds are dried, crush, cross 50-300 mesh sieves, obtain lotus nut starch；
(b) parts by weight of lotus nut starch 40 are weighed, 0.1-0.3 parts by volume 0.05-0.2mol/L sodium hydroxide solutions are added,
Stirring, stands overnight；
(c) upper strata for the reaction solution for preparing the step (b) is discarded, the water of lower sediment 0.08-0.12 parts by volume
Washing 2-4 times, is colourless to upper solution；
(d) reaction solution for preparing the step (c) is centrifuged, and centrifugal speed is 2000-4000r/min, during centrifugation
Between be 5-15min, upper solution is discarded, sediment E is obtained；
(e) the sediment E is washed 2-4 times with 0.04-0.06 parts by volume ether, obtains sediment F；
(f) the sediment F of 10 parts by weight is weighed, 0.05-0.2mol/L sodium hydroxide solution 0.04-0.06 bodies are added
Product part, stirring, 30-70 DEG C of heating 10-30min discards upper solution；
(g) water 0.04-0.06 parts by volume, centrifugation are added in the reaction solution prepared to the step (f), centrifugal speed is
2000-4000r/min, centrifugation time is 5-15min, discards upper solution, obtains sediment G；
(h) the sediment G is washed with water, the pH to upper solution is 7.0, obtains sediment H, 30-50 DEG C of drying, i.e.,
Obtain the lotus seed starch；
The relation of the parts by weight and parts by volume is g/L.
In the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention, in the step (b) and step (f), the hydrogen
The concentration of sodium hydroxide solution is 0.1mol/L.
In the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention, in the step (d) and/or the step (g),
The centrifugal speed is 3000r/min.
In the method for extraction and purification of the above-mentioned lotus seeds amylose of the present invention, in the step (h), the drying is dry for decompression
It is dry.
The above-mentioned technical proposal of the present invention has advantages below compared with prior art：
According to《The preparation of maize amylose and its characteristic》Record, (1) in alkaline solution, hydroxyl on starch molecule
Proton be dissociated, starch molecule is negatively charged, intermolecular mutually exclusive, with the increase of alkali concn, and this repulsion also accordingly increases
By force, the expansion even molecular degradation of helical region is ultimately resulted in；(2) under the effect of high speed centrifugation power so that the conformation of starch molecule
The molecular structure of especially amylose changes, and causes the binding ability of amylose and iodine molecule to decline, so straight chain
The blue value reduction of starch.With reference to《The separation of six kinds of lotus seed starch and its moral character characteristic research》This paper is understood, according to above-mentioned
Technical teaching, those skilled in the art form a kind of technology prejudice：Improve the concentration of NaOH solution and improve centrifugation rate, will
The molecular structure of lotus seeds amylose is destroyed, causes the binding ability of amylose and iodine molecule to decline, and then cause lotus seeds straight
The blue value reduction of chain starch.The method for extraction and purification of lotus seeds amylose of the prior art, often step is complicated, efficiency compared with
Low, long preparation period, it is difficult to accomplish scale production.
The method for extraction and purification of lotus seeds amylose of the present invention, applicant overcomes above-mentioned technology prejudice, by substantial amounts of
Creative work finds, using the lotus seed starch obtained by prepared by dry lotus seeds as raw material, by optimizing centrifugation rate and NaOH solution
Concentration etc., on the premise of lotus seeds amylose molecule structure is not destroyed, improves efficiency, shortens the operating time, is conducive to
The large-scale production of lotus seeds amylose, and the obtained lotus seeds amylose purity of institute's extraction purification is high (about 94%), can be with
It is used as the standard of lotus seeds amylose content determination method.
Brief description of the drawings
In order that present disclosure is more likely to be clearly understood, specific embodiment and combination below according to the present invention
Accompanying drawing, the present invention is further detailed explanation, wherein：
Fig. 1 is abosrption spectrogram of the lotus seeds amylose of the extraction purification of the embodiment of the present invention 3 after adding iodine colour developing.
Embodiment
1 instrument and reagent
1.1 instrument
Centrifuge Hitachi CR22G III；Vacuum drying chamber；Freeze drier；Water-bath；Acidometer；Refrigerator.
1.2 reagent
Lotus seeds are provided by Inst. of White Lotus Science, Guangchang County of Jiangxi Province；
Sodium hydroxide, hydrochloric acid, isopropanol, n-butanol, ether, KI, Potassiumiodate, absolute ethyl alcohol are commercially available product, point
Analysis is pure；Water is distilled water.
Experimental example 1
The absorption spectrum of iodo- lotus seeds amylose
The lotus seeds amylose 100mg of the extraction purification of embodiment 3 is weighed, is added in 100mL beakers, 95% ethanol is added
1.0mL infiltrates sample, then adds 1.0mol/L sodium hydroxide solution 9mL, sample is heated in 85 DEG C of water-baths, until sample is complete
It is scattered, cool down and constant volume is diluted with water into 100mL volumetric flasks, acutely shake 1min, it is standby.
The 1mg/mL lotus seeds amylose solution 2mL of above-mentioned preparation, plus 0.09mol/L sodium hydroxide solution 3mL are taken, plus
50mL water dilutes, and adds 1mol/L acetic acid solutions 1mL and iodine reagent 1mL, is settled to 100mL with water, and develop the color 10min, with point
Absorption spectrum interval light photometer scanning 500nm-800nm, as a result as shown in Figure 1.
As shown in Figure 1, a length of 631nm of maximum absorption wave of the lotus seeds amylose of the extraction purification of embodiment 3, with document report
The maximum absorption wavelength of the lotus seeds amylose in road is consistent.This shows that the product of the extraction purification of embodiment 3 forms sediment for lotus seeds straight chain
Powder.
Experimental example 2
The measure of lotus seeds amylose content
The lotus seeds amylose of the extraction purification of 0.1000g embodiments 3 is weighed, 0.5mol/L sodium hydroxide solutions are added
10mL, disperses in boiling water bath.Add 2mol/L hydrochloric acid 21.5mL, the back hydrolysis 2h in boiling water bath, by GB/T5513-
2008 determine reduced sugar, are multiplied by 0.9 and produce content of starch.
After measured, the lotus seeds amylose of the extraction purification of embodiment 3, to count content of starch as former state as 88.5%, with butt
It is 93.7% to count content of starch, and crude protein therein, three sums of ash content and fat content are less than 1%, and purity is higher, Ke Yizuo
For the standard of lotus seeds amylose content determination method.
Experimental example 3
The measure of lotus seeds amylose iodine binding capacity
Distinguish the lotus seeds amylose 0.1000g of Example 3, comparative example 1, comparative example 7 and the extraction purification of comparative example 8,
Add into 100mL volumetric flasks, plus after a few drop absolute ethyl alcohol moistenings, add 0.5mol/L sodium hydroxide solution 10mL, put boiling
Water-bath is completely dispersed, and the constant volume that added water after cooling is to scale.5mL (equivalent to containing amylose 5mg) dispersion liquids are drawn to burn in 200mL
In cup, plus 85mL water, 1mol/L hydrochloric acid 5mL and 0.4mol/L liquor kalii iodides 5mL.Beaker is placed on magnetic stirrer, will
Under platinum electrode and calomel electrode insertion liquid level, titrated with Potassiumiodate standard liquid, 0.1mL is added dropwise every time, plus rear 1min reads milli
Voltage.Titration end-point is calculated with second derivative method, and calculation formula is：Amylose iodine binding capacity (%)=0.6346 × V ×
100/m。
In formula：M- lotus seeds amylose quality, mg；V-8.34×10-4Mol/L potassium iodate solution titration volumes, mL；
Every milliliter of 0.6346- 8.34 × 10-4Iodine of the mol/L potassium iodate solutions equivalent to 0.6346mg.
The iodine binding capacity measurement result of the lotus seeds amylose of table 1
| Numbering | Iodine binding capacity (%) |
| Comparative example 1 | 9.90 |
| Comparative example 7 | 9.65 |
| Comparative example 8 | 10.15 |
| Embodiment 3 | 10.79 |
The measurement result of iodine binding capacity is as shown in table 1.As shown in Table 1, the lotus seeds amylose of the extraction purification of embodiment 3
Iodine binding capacity is maximum, far above the iodine binding capacity of the lotus seeds amylose of institute's extraction purification in comparative example 1,7,8.This shows, real
Apply the method for extraction and purification of example 3 influences minimum to the molecular structure of lotus seeds amylose.
Experimental example 4
The measure of lotus seeds amylose blue value
The lotus seeds amylose 0.1g of Example 3, comparative example 1, comparative example 7 and the extraction purification of comparative example 8, is made respectively
Concentration is 1mg/mL solution.
The 1mg/mL lotus seeds amylose solution 1mL of above-mentioned preparation, plus 35mL water are taken, with 0.05mol/L hydrochloric acid solution
Adjust pH to 3.0, plus 2% iodine-potassium iodide 0.5mL, constant volume is fully mixed to 50mL, used after 20min spectrophotometer in
500nm-700nm is scanned, and the wavelength at top is maximum absorption wavelength, and the absorbance at this is designated as Amax.
Calculation formula is as follows：The concentration of the sample of blue value=Amax × 4/.In formula, the unit of the concentration of sample is mg/
100mL。
The measurement result of the blue value of the lotus seeds amylose of table 2
| Numbering | Blue value |
| Comparative example 7 | 0.798 |
| Comparative example 8 | 0.850 |
| Embodiment 3 | 0.888 |
| Comparative example 1 | 0.856 |
The blue value measurement result of lotus seeds amylose is as shown in table 2.As shown in Table 2, the lotus seeds of the extraction purification of embodiment 3 are straight
The blue value of chain starch is maximum, far above the blue value of the lotus seeds amylose of institute's extraction purification in comparative example 1,7,8.This shows, real
Apply the method for extraction and purification of example 3 influences minimum to the molecular structure of lotus seeds amylose.
Experimental example 5
The measure of lotus seeds amylose absorbance
Difference Example 3, comparative example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5, comparative example 6, comparative example 7
After each 0.1g of lotus seeds amylose of the extraction purification of comparative example 8, plus iodine colour developing, its absorbance is determined, as a result as shown in table 3.
The measurement result of the absorbance of the lotus seeds amylose of table 3
| Numbering | Absorbance |
| Comparative example 1 | 0.388 |
| Comparative example 2 | 0.499 |
| Embodiment 3 | 0.408 |
| Comparative example 3 | 0.344 |
| Comparative example 4 | 0.343 |
| Comparative example 5 | 0.340 |
| Comparative example 6 | 0.342 |
| Comparative example 7 | 0.367 |
| Comparative example 8 | 0.386 |
As shown in Table 3, the absorbance of the lotus seeds amylose of the extraction purification of embodiment 3 is maximum.This shows, embodiment 3
Method for extraction and purification influences minimum to the molecular structure of lotus seeds amylose.
The method for extraction and purification of lotus seeds amylose of the present invention, using the lotus seed starch obtained by the preparation of dry lotus seeds as raw material, leads to
Concentration of optimization centrifugation rate and NaOH solution etc. is crossed, on the premise of lotus seeds amylose molecule structure is not destroyed, is improved
Efficiency, the large-scale production for shortening the operating time, being conducive to lotus seeds amylose, and the lotus seeds that institute's extraction purification is obtained are straight
Chain purity of starch height (about 94%), can as lotus seeds amylose content determination method standard.
Embodiment 1
A kind of method for extraction and purification of lotus seeds amylose of the present embodiment, comprises the following steps：
(a) after lotus seeds are dried, crush, cross 50 mesh sieves, obtain lotus nut starch；
(b) the lotus nut starch 40g is weighed, 0.1L 0.2mol/L sodium hydroxide solutions are added, stirring is stood overnight；
(c) upper strata of reaction solution for preparing the step (b) is discarded, lower sediment 0.08L water washing 4 times, extremely
Upper solution is colourless；
(d) reaction solution for preparing the step (c) is centrifuged, and centrifugal speed is 2000r/min, and centrifugation time is
15min, upper solution is discarded, and obtains sediment E；
(e) the sediment E is washed 4 times with 0.04L ether, obtains sediment F；
(f) the 10g sediment F is weighed, 0.05mol/L sodium hydroxide solution 0.06L, stirring, 30 DEG C of heating are added
30min, discards upper solution；
(g) water 0.04L is added in the reaction solution prepared to the step (f), centrifuged, centrifugal speed is 2000r/min, from
The heart time is 15min, discards upper solution, obtains sediment G；
(h) the sediment G is washed with water, the pH to upper solution is 7.0, obtains sediment H, 30 DEG C are dried under reduced pressure,
Produce the lotus seed starch；
(1) lotus seed starch 10g is weighed, 0.30L 0.8mol/L sodium hydroxide solution, boiling water bath heating stirring is added
15min, to being completely dispersed, is cooled to room temperature；
(2) reaction solution for preparing the step (1) is centrifuged, and centrifugal speed is 4000r/min, and centrifugation time is
25min, takes supernatant, and it is 6.0 that pH is adjusted to 1.0mol/L hydrochloric acid；
(3) the mixed liquor 0.06L of n-butanol/isoamyl alcohol, institute are added in the reaction solution prepared to step described in 0.32L (2)
The volume ratio for stating n-butanol and isoamyl alcohol in the mixed liquor of n-butanol/isoamyl alcohol is 1:1, boiling water bath heating stirring 20min, cooling
To room temperature, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 4000r/min, centrifuged by 2 DEG C of standing 30h
Time is 15min, obtains sediment A；
(4) the sediment A is washed with saturation n-butanol aqueous solution, centrifuged, centrifugal speed is 4000r/min, centrifuged
Time is 15min, obtains sediment B；
(5) sediment B is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring 10min is cooled to room
Temperature, 2 DEG C of standing 30h, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 4000r/min, centrifugation time
For 15min, sediment C is obtained；
(6) the sediment C is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring, purifies 4 times, obtain repeatedly
Sediment D；
(7) sediment D is washed 2 times with ethanol, freeze-drying is dried, and produces lotus seeds amylose.
Embodiment 2
A kind of method for extraction and purification of lotus seeds amylose of the present embodiment, comprises the following steps：
(a) after lotus seeds are dried, crush, cross 300 mesh sieves, obtain lotus nut starch；
(b) the lotus nut starch 40g is weighed, 0.3L 0.05mol/L sodium hydroxide solutions are added, stirring is stood overnight；
(c) upper strata of reaction solution for preparing the step (b) is discarded, lower sediment 0.12L water washing 2 times, extremely
Upper solution is colourless；
(d) reaction solution for preparing the step (c) is centrifuged, and centrifugal speed is 4000r/min, and centrifugation time is
5min, upper solution is discarded, and obtains sediment E；
(e) the sediment E is washed 2 times with 0.06L ether, obtains sediment F；
(f) the 10g sediment F is weighed, 0.2mol/L sodium hydroxide solution 0.04L, stirring, 70 DEG C of heating are added
10min, discards upper solution；
(g) water 0.06L is added in the reaction solution prepared to the step (f), centrifuged, centrifugal speed is 4000r/min, from
The heart time is 5min, discards upper solution, obtains sediment G；
(h) the sediment G is washed with water, the pH to upper solution is 7.0, obtains sediment H, 50 DEG C are dried under reduced pressure,
Produce the lotus seed starch；
(1) lotus seed starch 10g is weighed, 0.40L 0.5mol/L sodium hydroxide solution, boiling water bath heating stirring is added
25min, to being completely dispersed, is cooled to room temperature；
(2) reaction solution for preparing the step (1) is centrifuged, and centrifugal speed is 18000r/min, and centrifugation time is
15min, takes supernatant, and it is 7.0 that pH is adjusted to 2.0mol/L hydrochloric acid；
(3) the mixed liquor 0.10L of n-butanol/isoamyl alcohol, institute are added in the reaction solution prepared to step described in 0.61L (2)
The volume ratio for stating n-butanol and isoamyl alcohol in the mixed liquor of n-butanol/isoamyl alcohol is 1:1, boiling water bath heating stirring 10min, cooling
To room temperature, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 18000r/min, centrifuged by 4 DEG C of standing 20h
Time is 5min, obtains sediment A；
(4) the sediment A is washed with saturation n-butanol aqueous solution, centrifuged, centrifugal speed is 18000r/min, centrifuged
Time is 5min, obtains sediment B；
(5) sediment B is placed in saturation n-butanol aqueous solution, heating stirring 20min, is cooled to room temperature, 4 DEG C quiet
20h is put, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 18000r/min, centrifugation time is 5min,
Obtain sediment C；
(6) the sediment C is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring, purifies 6 times, obtain repeatedly
Sediment D；
(7) sediment D is washed 5 times with ethanol, freeze-drying is dried, and produces lotus seeds amylose.
Embodiment 3
A kind of method for extraction and purification of lotus seeds amylose of the present embodiment, comprises the following steps：
(a) after lotus seeds are dried, crush, cross 60 mesh sieves, obtain lotus nut starch；
(b) the lotus nut starch 40g is weighed, 0.2L 0.1mol/L sodium hydroxide solutions are added, stirring is stood overnight；
(c) upper strata of reaction solution for preparing the step (b) is discarded, lower sediment 0.1L water washing 2 times, supreme
Layer solution is colourless；
(d) reaction solution for preparing the step (c) is centrifuged, and centrifugal speed is 3000r/min, and centrifugation time is
10min, upper solution is discarded, and obtains sediment E；
(e) the sediment E is washed 3 times with 0.05L ether, obtains sediment F；
(f) the 10g sediment F is weighed, 0.1mol/L sodium hydroxide solution 0.05L, stirring, 50 DEG C of heating are added
20min, discards upper solution；
(g) water 0.05L is added in the reaction solution prepared to the step (f), centrifuged, centrifugal speed is 3000r/min, from
The heart time is 10min, discards upper solution, obtains sediment G；
(h) the sediment G is washed with water, the pH to upper solution is 7.0, obtains sediment H, 40 DEG C are dried under reduced pressure,
Produce the lotus seed starch；
(1) lotus seed starch 10g is weighed, 0.35L 0.6mol/L sodium hydroxide solution, boiling water bath heating stirring is added
20min, to being completely dispersed, is cooled to room temperature；
(2) reaction solution for preparing the step (1) is centrifuged, and centrifugal speed is 7000r/min, and centrifugation time is
20min, takes supernatant, and it is 6.5 that pH is adjusted to 1.5mol/L hydrochloric acid；
(3) the mixed liquor 0.08L of n-butanol/isoamyl alcohol, institute are added in the reaction solution prepared to step described in 0.46L (2)
The volume ratio for stating n-butanol and isoamyl alcohol in the mixed liquor of n-butanol/isoamyl alcohol is 1:1, boiling water bath heating stirring 15min, cooling
To room temperature, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 16000r/min, centrifuged by 3 DEG C of standing 24h
Time is 10min, obtains sediment A；
(4) the sediment A is washed with saturation n-butanol aqueous solution, centrifuged, centrifugal speed is 12000r/min, centrifuged
Time is 5min, obtains sediment B；
(5) sediment B is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring 15min is cooled to room
Temperature, 3 DEG C of standing 24h, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 16000r/min, centrifugation time
For 10min, sediment C is obtained；
(6) the sediment C is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring, purifies 5 times, obtain repeatedly
Sediment D；
(7) sediment D is washed 4 times with ethanol, freeze-drying is dried, and produces lotus seeds amylose.
Embodiment 4
A kind of method for extraction and purification of lotus seeds amylose of the present embodiment, comprises the following steps：
(a) after lotus seeds are dried, crush, cross 60 mesh sieves, obtain lotus nut starch；
(b) the lotus nut starch 40g is weighed, 0.2L 0.1mol/L sodium hydroxide solutions are added, stirring is stood overnight；
(c) upper strata of reaction solution for preparing the step (b) is discarded, lower sediment 0.1L water washing 2 times, supreme
Layer solution is colourless；
(d) reaction solution for preparing the step (c) is centrifuged, and centrifugal speed is 3000r/min, and centrifugation time is
10min, upper solution is discarded, and obtains sediment E；
(e) the sediment E is washed 2 times with 0.05L ether, obtains sediment F；
(f) the 10g sediment F is weighed, 0.1mol/L sodium hydroxide solution 0.05L, stirring, 50 DEG C of heating are added
20min, discards upper solution；
(g) water 0.05L is added in the reaction solution prepared to the step (f), centrifuged, centrifugal speed is 3000r/min, from
The heart time is 10min, discards upper solution, obtains sediment G；
(h) the sediment G is washed with water, the pH to upper solution is 7.0, obtains sediment H, 40 DEG C are dried under reduced pressure,
Produce the lotus seed starch；
(1) lotus seed starch 10g is weighed, 0.35L 0.6mol/L sodium hydroxide solution, boiling water bath heating stirring is added
20min, to being completely dispersed, is cooled to room temperature；
(2) reaction solution for preparing the step (1) is centrifuged, and centrifugal speed is 18000r/min, and centrifugation time is
20min, takes supernatant, and it is 6.5 that pH is adjusted to 1.5mol/L hydrochloric acid；
(3) the mixed liquor 0.08L of n-butanol/isoamyl alcohol, institute are added in the reaction solution prepared to step described in 0.46L (2)
The volume ratio for stating n-butanol and isoamyl alcohol in the mixed liquor of n-butanol/isoamyl alcohol is 1:1, boiling water bath heating stirring 15min, cooling
To room temperature, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 18000r/min, centrifuged by 3 DEG C of standing 24h
Time is 10min, obtains sediment A；
(4) the sediment A is washed with saturation n-butanol aqueous solution, centrifuged, centrifugal speed is 18000r/min, centrifuged
Time is 5min, obtains sediment B；
(5) sediment B is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring 15min is cooled to room
Temperature, 3 DEG C of standing 24h, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 18000r/min, centrifugation time
For 10min, sediment C is obtained；
(6) the sediment C is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring, purifies 5 times, obtain repeatedly
Sediment D；
(7) sediment D is washed 4 times with ethanol, freeze-drying is dried, and produces lotus seeds amylose.
Embodiment 5
A kind of method for extraction and purification of lotus seeds amylose of the present embodiment, comprises the following steps：
(a) after lotus seeds are dried, crush, cross 60 mesh sieves, obtain lotus nut starch；
(b) the lotus nut starch 40g is weighed, 0.2L 0.1mol/L sodium hydroxide solutions are added, stirring is stood overnight；
(c) upper strata of reaction solution for preparing the step (b) is discarded, lower sediment 0.1L water washing 2 times, supreme
Layer solution is colourless；
(d) reaction solution for preparing the step (c) is centrifuged, and centrifugal speed is 3000r/min, and centrifugation time is
10min, upper solution is discarded, and obtains sediment E；
(e) the sediment E is washed 2 times with 0.05L ether, obtains sediment F；
(f) the 10g sediment F is weighed, 0.1mol/L sodium hydroxide solution 0.05L, stirring, 50 DEG C of heating are added
20min, discards upper solution；
(g) water 0.05L is added in the reaction solution prepared to the step (f), centrifuged, centrifugal speed is 3000r/min, from
The heart time is 10min, discards upper solution, obtains sediment G；
(h) the sediment G is washed with water, the pH to upper solution is 7.0, obtains sediment H, 40 DEG C are dried under reduced pressure,
Produce the lotus seed starch；
(1) lotus seed starch 10g is weighed, 0.35L 0.6mol/L sodium hydroxide solution, boiling water bath heating stirring is added
20min, to being completely dispersed, is cooled to room temperature；
(2) reaction solution for preparing the step (1) is centrifuged, and centrifugal speed is 10000r/min, and centrifugation time is
20min, takes supernatant, and it is 6.5 that pH is adjusted to 1.5mol/L hydrochloric acid；
(3) the mixed liquor 0.08L of n-butanol/isoamyl alcohol, institute are added in the reaction solution prepared to step described in 0.46L (2)
The volume ratio for stating n-butanol and isoamyl alcohol in the mixed liquor of n-butanol/isoamyl alcohol is 1:1, boiling water bath heating stirring 15min, cooling
To room temperature, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 10000r/min, centrifuged by 3 DEG C of standing 24h
Time is 10min, obtains sediment A；
(4) the sediment A is washed with saturation n-butanol aqueous solution, centrifuged, centrifugal speed is 10000r/min, centrifuged
Time is 5min, obtains sediment B；
(5) sediment B is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring 15min is cooled to room
Temperature, 3 DEG C of standing 24h, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 10000r/min, centrifugation time
For 10min, sediment C is obtained；
(6) the sediment C is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring, purifies 5 times, obtain repeatedly
Sediment D；
(7) sediment D is washed 4 times with ethanol, freeze-drying is dried, and produces lotus seeds amylose.
Comparative example 1
The method for extraction and purification of the present embodiment lotus seeds amylose and the method for extraction and purification of the lotus seeds amylose of embodiment 3
Each step operating parameter all same, differ only in：The concentration of sodium hydroxide solution is 0.7mol/L in step (1).
Comparative example 2
The method for extraction and purification of the present embodiment lotus seeds amylose and the method for extraction and purification of the lotus seeds amylose of embodiment 3
Each step operating parameter all same, differ only in：Dried in step (7) using boulton process.
Comparative example 3
The method for extraction and purification of the present embodiment lotus seeds amylose and the method for extraction and purification of the lotus seeds amylose of embodiment 3
Each step operating parameter all same, differ only in：In step (2), step (3), step (4) and step (5), centrifugation speed
Degree is 3000r/min.
Comparative example 4
The method for extraction and purification of the present embodiment lotus seeds amylose and the method for extraction and purification of the lotus seeds amylose of embodiment 3
Each step operating parameter all same, differ only in：In step (2), step (3), step (4) and step (5), centrifugation speed
Degree is 4000r/min.
Comparative example 5
The method for extraction and purification of the present embodiment lotus seeds amylose and the method for extraction and purification of the lotus seeds amylose of embodiment 3
Each step operating parameter all same, differ only in：In step (2), step (3), step (4) and step (5), centrifugation speed
Degree is 5000r/min.
Comparative example 6
The method for extraction and purification of the present embodiment lotus seeds amylose and the method for extraction and purification of the lotus seeds amylose of embodiment 3
Each step operating parameter all same, differ only in：In step (2), step (3), step (4) and step (5), centrifugation speed
Degree is 16000r/min.
Comparative example 7
The method for extraction and purification of the present embodiment lotus seeds amylose and the method for extraction and purification of the lotus seeds amylose of embodiment 3
Each step operating parameter all same, differ only in：The concentration of sodium hydroxide solution is 0.4mol/L in step (1).
Comparative example 8
The method for extraction and purification of the present embodiment lotus seeds amylose and the method for extraction and purification of the lotus seeds amylose of embodiment 3
Each step operating parameter all same, differ only in：The concentration of sodium hydroxide solution is 0.5mol/L in step (1).
Obviously, above-described embodiment is only intended to clearly illustrate example, and the not restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (1)
1. a kind of method for extraction and purification of lotus seeds amylose, comprises the following steps：
(a) after lotus seeds are dried, crush, cross 60 mesh sieves, obtain lotus nut starch；
(b) the lotus nut starch 40g is weighed, 0.2L 0.1mol/L sodium hydroxide solutions are added, stirring is stood overnight；
(c) upper strata of reaction solution for preparing the step (b) is discarded, lower sediment 0.1L water washing 2 times, molten to upper strata
Liquid is colourless；
(d) reaction solution for preparing the step (c) is centrifuged, and centrifugal speed is 3000r/min, and centrifugation time is 10min,
Upper solution is discarded, sediment E is obtained；
(e) the sediment E is washed 3 times with 0.05L ether, obtains sediment F；
(f) the 10g sediment F is weighed, 0.1mol/L sodium hydroxide solution 0.05L are added, stirred, 50 DEG C of heating 20min,
Discard upper solution；
(g) water 0.05L is added in the reaction solution prepared to the step (f), centrifuged, centrifugal speed is 3000r/min, during centrifugation
Between be 10min, discard upper solution, obtain sediment G；
(h) the sediment G is washed with water, the pH to upper solution is 7.0, obtains sediment H, 40 DEG C are dried under reduced pressure, and produce
The lotus seed starch；
(1) weigh lotus seed starch 10g, add 0.35L 0.6mol/L sodium hydroxide solution, boiling water bath heating stirring 20min,
To being completely dispersed, room temperature is cooled to；
(2) reaction solution for preparing the step (1) is centrifuged, and centrifugal speed is 7000r/min, and centrifugation time is 20min,
Supernatant is taken, it is 6.5 that pH is adjusted to 1.5mol/L hydrochloric acid；
(3) add the mixed liquor 0.08L of n-butanol/isoamyl alcohol in the reaction solution prepared to step described in 0.46L (2), it is described just
The volume ratio of n-butanol and isoamyl alcohol is 1 in the mixed liquor of butanol/isoamyl alcohol:1, boiling water bath heating stirring 15min, are cooled to room
Temperature, 3 DEG C of standing 24h, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 16000r/min, centrifugation time
For 10min, sediment A is obtained；
(4) the sediment A is washed with saturation n-butanol aqueous solution, centrifuged, centrifugal speed is 12000r/min, centrifugation time
For 5min, sediment B is obtained；
(5) sediment B is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring 15min is cooled to room temperature, 3 DEG C
24h is stood, upper solution is discarded, lower sediment is centrifuged, centrifugal speed is 16000r/min, centrifugation time is
10min, obtains sediment C；
(6) the sediment C is placed in saturation n-butanol aqueous solution, boiling water bath heating stirring, purifies 5 times, must precipitate repeatedly
Thing D；
(7) sediment D is washed 4 times with ethanol, freeze-drying is dried, and produces lotus seeds amylose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510263729.2A CN104892777B (en) | 2015-05-22 | 2015-05-22 | A kind of method for extraction and purification of lotus seeds amylose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510263729.2A CN104892777B (en) | 2015-05-22 | 2015-05-22 | A kind of method for extraction and purification of lotus seeds amylose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104892777A CN104892777A (en) | 2015-09-09 |
| CN104892777B true CN104892777B (en) | 2017-11-07 |
Family
ID=54025782
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510263729.2A Expired - Fee Related CN104892777B (en) | 2015-05-22 | 2015-05-22 | A kind of method for extraction and purification of lotus seeds amylose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104892777B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109651515A (en) * | 2018-12-10 | 2019-04-19 | 北京工商大学 | A method of processing lotus seeds scarlet reground grain |
| CN114989316A (en) * | 2022-06-01 | 2022-09-02 | 山东省农业科学院 | Method for obtaining high amylose starch from cashew kernels |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101518343A (en) * | 2009-04-02 | 2009-09-02 | 福建农林大学 | Method for preparing lotus seed starch sponge |
| CN104628873A (en) * | 2015-02-10 | 2015-05-20 | 天津大学 | Method for separating high-solubility pueraria starch from pueraria powder |
-
2015
- 2015-05-22 CN CN201510263729.2A patent/CN104892777B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN104892777A (en) | 2015-09-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102960644A (en) | Preparation method of pumpkin dietary fiber and pumpkin dietary fiber | |
| CN103006899B (en) | Medlar general flavones extracting and purifying technology and medlar general flavone detecting method as well as applications of medlar general flavone | |
| CN103416639B (en) | The method of the quick debitterize of a kind of supersonic induced semen armeniacae amarae | |
| CN103435714B (en) | The preparation of the thick polysaccharide of a kind of fig and purification process and purposes | |
| CN101891840B (en) | Method for extracting pectin by utilizing potato scraps | |
| CN104892777B (en) | A kind of method for extraction and purification of lotus seeds amylose | |
| CN103804507A (en) | Maryland tobacco polysaccharide, extracting and purifying method and application thereof as antioxidant | |
| CN105175573A (en) | High-efficiency radix astragali polysaccharide extraction method | |
| CN102863509B (en) | Method for extracting protein from potato starch processing liquid waste | |
| CN106722943A (en) | A kind of apple ferment and preparation method thereof | |
| CN109965173B (en) | Preparation method and application of phycoerythrin, polysaccharide and dietary fiber extracted from laver in water powder | |
| CN109134695B (en) | Alcohol-soluble beta-glucan extract, preparation method and application thereof, and health-care wine | |
| CN103169107A (en) | Preparation method for nutrition ingredients of kelp seaweed | |
| WO2019184320A1 (en) | Method for co-producing gelatinous and emulsified pectin by using cichorium intybus/helianthus tuberosus meal | |
| CN108740219A (en) | The preparation method and its usage of miracle fruit leaf soaking object and function tea beverage | |
| CN101824097B (en) | Method for producing polyglucose | |
| CN102372795A (en) | Method for preparing beta-chitin by utilizing squid theca | |
| CN103911273B (en) | A kind of liquid fermentation embryo nelumbinis-germination rice vinegar and preparation technology thereof | |
| CN112724276A (en) | Ultrasonic-assisted extraction process for optimizing aralia leucotaxis stem bark polysaccharide based on response surface method | |
| CN108003252A (en) | It is a kind of that there is sea grass polysaccharide carboxy methylation modified outcome for improving antioxidation activity and preparation method thereof | |
| CN109468197A (en) | A kind of novel sealwort wine and its production technology | |
| CN105295434B (en) | Method for combined extraction of haematochrome and pectin from pitaya peel | |
| CN103183740A (en) | Production method of fructus schisandrae polysaccharide | |
| CN106177499A (en) | A kind of Herba Dendrobii total flavone extracting method | |
| CN101611837A (en) | The method of preparing sweet potato amylodextrin by acid-alcohol medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171107 |