CN104892777A - Extracting and purifying method for amylase of lotus seeds - Google Patents
Extracting and purifying method for amylase of lotus seeds Download PDFInfo
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- 240000002853 Nelumbo nucifera Species 0.000 title claims abstract description 157
- 235000006508 Nelumbo nucifera Nutrition 0.000 title claims abstract description 157
- 235000006510 Nelumbo pentapetala Nutrition 0.000 title claims abstract description 157
- 238000000034 method Methods 0.000 title claims abstract description 74
- 239000004382 Amylase Substances 0.000 title abstract 9
- 102000013142 Amylases Human genes 0.000 title abstract 9
- 108010065511 Amylases Proteins 0.000 title abstract 9
- 235000019418 amylase Nutrition 0.000 title abstract 9
- 229920002472 Starch Polymers 0.000 claims abstract description 137
- 235000019698 starch Nutrition 0.000 claims abstract description 137
- 239000008107 starch Substances 0.000 claims abstract description 137
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 114
- 239000000243 solution Substances 0.000 claims description 132
- 229920000856 Amylose Polymers 0.000 claims description 119
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 57
- 239000013049 sediment Substances 0.000 claims description 49
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 47
- 238000005119 centrifugation Methods 0.000 claims description 45
- 238000006243 chemical reaction Methods 0.000 claims description 37
- 238000009835 boiling Methods 0.000 claims description 28
- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 claims description 24
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 23
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 23
- 239000007864 aqueous solution Substances 0.000 claims description 22
- 238000001035 drying Methods 0.000 claims description 20
- 239000011259 mixed solution Substances 0.000 claims description 17
- 238000005406 washing Methods 0.000 claims description 15
- 239000000843 powder Substances 0.000 claims description 14
- 210000000582 semen Anatomy 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 13
- 238000010438 heat treatment Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 8
- 229960004132 diethyl ether Drugs 0.000 claims description 7
- 238000004108 freeze drying Methods 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 abstract description 7
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 3
- 238000004904 shortening Methods 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 38
- 238000000605 extraction Methods 0.000 description 16
- 238000000746 purification Methods 0.000 description 16
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 11
- 229910052740 iodine Inorganic materials 0.000 description 11
- 239000011630 iodine Substances 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- JLKDVMWYMMLWTI-UHFFFAOYSA-M potassium iodate Chemical compound [K+].[O-]I(=O)=O JLKDVMWYMMLWTI-UHFFFAOYSA-M 0.000 description 4
- 235000006666 potassium iodate Nutrition 0.000 description 4
- 239000001230 potassium iodate Substances 0.000 description 4
- 229940093930 potassium iodate Drugs 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 229960000935 dehydrated alcohol Drugs 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000004448 titration Methods 0.000 description 3
- 229920000945 Amylopectin Polymers 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 1
- 241000209447 Nelumbo Species 0.000 description 1
- 235000005807 Nelumbo Nutrition 0.000 description 1
- 206010059013 Nocturnal emission Diseases 0.000 description 1
- 244000213382 Nymphaea lotus Species 0.000 description 1
- 235000010710 Nymphaea lotus Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 102000011759 adducin Human genes 0.000 description 1
- 108010076723 adducin Proteins 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000003637 basic solution Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical compound Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229960004756 ethanol Drugs 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 235000007715 potassium iodide Nutrition 0.000 description 1
- 229960004839 potassium iodide Drugs 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000002522 swelling effect Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
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- Polysaccharides And Polysaccharide Derivatives (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention belongs to the field of biological chemistry and concretely relates to an extracting and purifying method for amylase of lotus seeds. In order to solve the technical problems of complex step, relatively-low efficiency, long preparation period and difficulty in large-scale production of the existing extracting and purifying method for amylase of lotus seeds, the invention provides an extracting and purifying method for amylase of lotus seeds, which is simple in process, capable of easily realizing large-scale production and high in yield and purity. According to the extracting and purifying method for amylase of lotus seeds, lotus seed starch prepared from dry lotus seeds is used as a raw material, and the centrifugal speed and the concentration and the like of a NaOH solution are optimized, so that the aims of increasing the efficiency, shortening the operation time and favorably realizing large-scale production of the amylase of lotus seeds are achieved on the premise that the molecular structure of the amylase of lotus seeds is not destroyed; in addition, the purity of the extracted and purified amylase of lotus seeds is high (about 94%) and can be used as the standard of a method for measuring the content of the amylase of lotus seeds.
Description
Technical field
The invention belongs to biochemical field, be specifically related to a kind of extracting and purifying method of lotus seeds amylose starch.
Background technology
Lotus seeds are Nymphaeceae Nelumbo (the Nelum bonucifera Gaertn) fruit of plant and the general name of seed, mainly be distributed in the ground such as Jiangxi, Fujian, Hunan, Hubei, be the important local product resource of China, there is abundant nutritive and health protection components and pharmacologically active.According to the record of Compendium of Material Medica, lotus seeds have bowl spares repose, quench the thirst reduce phlegm and internal heat, feel at ease only dysentery, lay one's heart bear kidney controlling nocturnal emission with astringent drugs gas, strengthening the bones and muscles, tonifying deficiency, sharp knowledge, eliminating cold-damp and woman be with effects such as collapsing.
Starch is the main component of lotus seeds, accounts for more than 50% of its dry-matter.Starch with most plants is the same, and lotus seed starch is also made up of amylose starch and amylopectin.Kind is different, different lotus seeds of originating, and its amylose content difference is larger.Lotus seeds amylose content is the principal element affecting the solvability of solid matter in lotus seeds water-absorbent, swelling property, boiling, color, gloss, viscosity and flexibility, is also the important factor determining Cooking Quality.
The molecular structure of lotus seeds amylose starch is the internal factor determining its quality and quality, and molecular structure is different, and its quality and quality are also different.And extracting and purifying method is the important factor of the molecular structure affecting lotus seeds amylose starch, it is also the important factor affecting its quality and quality.Under the prerequisite not destroying lotus seeds amylose molecule structure, the extracting and purifying method of research lotus seeds amylose starch, not only be conducive to the composition studying lotus seed starch, and be conducive to studying the corresponding relation between lotus seeds amylose content and lotus seeds quality, thus provide fundamental basis for the formulation of the seed selection of Lotus Seed Varieties, the exploitation of lotus seeds resource and lotus seeds quality relevant criterion.
Master thesis " separation of six kinds of lotus seed starch and quality characteristic research thereof " (Tao Jinhong, Nanjing Normal University, 2009) a kind of extracting and purifying method of lotus seeds amylose starch is disclosed, that is: take 10g starch and cross 40 mesh sieves, put into 500mL beaker, add a small amount of dehydrated alcohol and distilled water, make sample moistening, adding the NaOH 350mL of 0.5mol/L, in boiling water bath, heated and stirred 20 ~ 30min is to disperseing completely, after cooling centrifugal (4000r/min, 20min), go precipitation, then be adjusted to neutrality with 2mol/L HCl.Add 100mL propyl carbinol and primary isoamyl alcohol (volume ratio 3:1) mixed solution, then in boiling water bath, heated and stirred 10min is transparent to solution, be cooled to room temperature, move into 4 DEG C of refrigerators and leave standstill 24 rear centrifugal (4000r/min, 20min), the precipitation obtained is thick amylose starch (Am), and supernatant liquor is thick amylopectin solution (Ap).200mL propyl carbinol saturated aqueous solution is added in precipitation, then in boiling water bath, heated and stirred is transparent to Solution Dispersion, is cooled to room temperature, at 4 DEG C of refrigerator 24h, centrifugal (4000r/min, 20min), be precipitated, after repeating aforesaid operations 5 times, collect the throw out obtained and be dipped in 24h in dehydrated alcohol, and with absolute ethanol washing several, dry at 40 DEG C, obtain amylose starch (Am) sample.
But in the production process of reality, the step of the extracting and purifying method of above-mentioned lotus seeds amylose starch is complicated, efficiency is lower, and preparation cycle is long, is difficult to accomplish scale production.
Therefore, under the prerequisite not destroying lotus seeds amylose molecule structure, how research improves the efficiency of the extracting and purifying method of lotus seeds amylose starch, and shorten the process time, the large-scale production for lotus seeds amylose starch has very important significance.
Summary of the invention
For this reason; the technical problem being that the step of the extracting and purifying method of existing lotus seeds amylose starch is complicated, efficiency is lower, preparation cycle is long, not easily accomplishing scale production to be solved by this invention, thus the extracting and purifying method of proposition a kind of lotus seeds amylose starch that technique simply, is easily accomplished scale production, productive rate is high, purity is high.
For solving the problems of the technologies described above, the present invention is achieved through the following technical solutions:
The extracting and purifying method of a kind of lotus seeds amylose starch of the present invention, comprises the following steps:
(1) taking lotus seed starch 10 weight part, add the sodium hydroxide solution of 0.30-0.40 parts by volume 0.5-0.8mol/L, heated and stirred 15-25min, to disperseing completely, being cooled to room temperature;
(2) undertaken centrifugal by reaction solution prepared by described step (1), centrifugal speed is 4000-18000r/min, and centrifugation time is 15-25min, gets supernatant liquor, and being adjusted to pH with 1.0-2.0mol/L hydrochloric acid is 6.0-7.0;
(3) the mixed solution 0.06-0.10 parts by volume of propyl carbinol/primary isoamyl alcohol is added in the reaction solution prepared to described step (2), heated and stirred 10-20min, be cooled to room temperature, 2-4 DEG C of standing 20-30h, upper solution discarded, undertaken centrifugal by lower sediment, centrifugal speed is 4000-18000r/min, centrifugation time is 5-15min, obtains throw out A;
(4) the saturated n-butanol aqueous solution of described throw out A is washed, centrifugal, centrifugal speed is 4000-18000r/min, and centrifugation time is 5-15min, obtains sediment B;
(5) described sediment B is placed in saturated n-butanol aqueous solution, heated and stirred 10-20min, is cooled to room temperature, 2-4 DEG C of standing 20-30h, discards upper solution, is undertaken centrifugal by lower sediment, centrifugal speed is 4000-18000r/min, and centrifugation time is 5-15min, obtains throw out C;
(6) described throw out C is placed in saturated n-butanol aqueous solution, heated and stirred, purifying 4-6 time, obtains sediment D repeatedly;
(7) by described sediment D washing with alcohol 2-5 time, dry, obtain lotus seeds amylose starch;
The pass of described weight part and parts by volume is g/L.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in at least one step of described step (2), step (3), step (4) and step (5), described centrifugal speed is 10000-18000r/min.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in described step (1), the concentration of described sodium hydroxide solution is 0.6mol/L or 0.7mol/L.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in described step (1), step (3), step (5) and step (6), described in be heated to be boiling water bath heating.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in described step (3) and step (5), the described standing time is 24h.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in described step (3), in the mixed solution of described propyl carbinol/primary isoamyl alcohol, the volume ratio of propyl carbinol and primary isoamyl alcohol is 1:1, and the volume ratio of reaction solution prepared by the mixed solution of described propyl carbinol/primary isoamyl alcohol and described step (2) is 1:5.25-6.125.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in described step (7), described drying adopts freeze-drying or boulton process.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in described step (1), described lotus seed starch obtains as follows:
A (), by after lotus seeds drying, pulverizes, cross 50-300 mesh sieve, obtain Semen Nelumbinis powder;
B () takes described Semen Nelumbinis powder 40 weight part, add 0.1-0.3 parts by volume 0.05-0.2mol/L sodium hydroxide solution, stirs, hold over night;
C the upper strata of reaction solution prepared by described step (b) discards by (), lower sediment water washing 2-4 time of 0.08-0.12 parts by volume is colourless to upper solution;
D reaction solution that described step (c) is prepared by () carries out centrifugal, and centrifugal speed is 2000-4000r/min, and centrifugation time is 5-15min, upper solution is discarded, and obtains throw out E;
E (), by described throw out E 0.04-0.06 parts by volume washed with diethylether 2-4 time, obtains throw out F;
F () takes the described throw out F of 10 weight parts, add 0.05-0.2mol/L sodium hydroxide solution 0.04-0.06 parts by volume, stirs, and 30-70 DEG C of heating 10-30min, discards upper solution;
Add water 0.04-0.06 parts by volume in g reaction solution that () prepares to described step (f), centrifugal, centrifugal speed is 2000-4000r/min, and centrifugation time is 5-15min, discards upper solution, obtains throw out G;
H described throw out G washes with water by (), the pH to upper solution is 7.0, obtains throw out H, and 30-50 DEG C of drying, obtains described lotus seed starch;
The pass of described weight part and parts by volume is g/L.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, described step (b) is with in step (f), and the concentration of described sodium hydroxide solution is 0.1mol/L.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in described step (d) and/or described step (g), described centrifugal speed is 3000r/min.
In the extracting and purifying method of the above-mentioned lotus seeds amylose starch of the present invention, in described step (h), described drying is drying under reduced pressure.
Technique scheme of the present invention has the following advantages compared to existing technology:
According to the record of " preparation of maize amylose and characteristic thereof ", (1) in basic solution, on starch molecule, the proton of hydroxyl is dissociated, starch molecule is electronegative, intermolecular mutual repulsion, along with the increase of alkali concn, this repulsion is corresponding enhancing also, finally causes the expansion even molecular degradation of helical region; (2) under the effect of high speed centrifugation power, the molecular structure of the conformation of starch molecule especially amylose starch is changed, causes the binding ability of amylose starch and iodine molecule to decline, so the blue value of amylose starch reduces.Known in conjunction with " separation of six kinds of lotus seed starch and moral character characteristic research thereof " this section of paper, instruct according to above-mentioned technology, those skilled in the art define a kind of technology prejudice: improve the concentration of NaOH solution and improve centrifugation rate, the molecular structure of lotus seeds amylose starch will be destroyed, cause the binding ability of amylose starch and iodine molecule to decline, and then cause the blue value of lotus seeds amylose starch to reduce.The extracting and purifying method of lotus seeds amylose starch of the prior art, often step is complicated, efficiency is lower, preparation cycle is long, not easily accomplish scale production.
The extracting and purifying method of lotus seeds amylose starch of the present invention; applicant overcomes above-mentioned technology prejudice; found by a large amount of creative works; the lotus seed starch of gained is prepared for raw material with dry lotus seeds; by optimizing the concentration etc. of centrifugation rate and NaOH solution; under the prerequisite not destroying lotus seeds amylose molecule structure; improve efficiency, shorten the operating time, be conducive to the large-scale production of lotus seeds amylose starch; and the lotus seeds amylose starch purity high (about 94%) that institute's extraction purification obtains, can as the standard of lotus seeds amylose content determination method.
Accompanying drawing explanation
In order to make content of the present invention be more likely to be clearly understood, below according to a particular embodiment of the invention and by reference to the accompanying drawings, the present invention is further detailed explanation, wherein:
Fig. 1 is the abosrption spectrogram of lotus seeds amylose starch after adding iodine colour developing of the embodiment of the present invention 3 extraction purification.
Embodiment
1 instrument and reagent
1.1 instrument
Whizzer Hitachi CR22G III; Vacuum drying oven; Freeze drier; Water-bath; Acidometer; Refrigerator.
1.2 reagent
Lotus seeds are provided by Inst. of White Lotus Science, Guangchang County of Jiangxi Province;
Sodium hydroxide, hydrochloric acid, Virahol, propyl carbinol, ether, potassiumiodide, Potassium Iodate, dehydrated alcohol are commercially available product, analytical pure; Water is distilled water.
experimental example 1
The absorption spectrum of iodo-lotus seeds amylose starch
Take the lotus seeds amylose starch 100mg of embodiment 3 extraction purification, join in 100mL beaker, add 95% ethanol 1.0mL and infiltrate sample, then 1.0mol/L sodium hydroxide solution 9mL is added, sample heats in 85 DEG C of water-baths, until sample disperses completely, cooling and dilute with water constant volume in 100mL volumetric flask, violent jolting 1min, for subsequent use.
Get the 1mg/mL lotus seeds amylose solution 2mL of above-mentioned preparation, add 0.09mol/L sodium hydroxide solution 3mL, add the dilution of 50mL water, add 1mol/L acetic acid solution 1mL and iodine reagent 1mL again, 100mL is settled to water, colour developing 10min, with the absorption spectrum in spectrophotometer scanning 500nm-800nm interval, result as shown in Figure 1.
As shown in Figure 1, the maximum absorption wavelength of the lotus seeds amylose starch of embodiment 3 extraction purification is 631nm, consistent with the maximum absorption wavelength of the lotus seeds amylose starch of bibliographical information.This shows, the product of embodiment 3 extraction purification is lotus seeds amylose starch.
experimental example 2
The mensuration of lotus seeds amylose content
Take the lotus seeds amylose starch of 0.1000g embodiment 3 extraction purification, add 0.5mol/L sodium hydroxide solution 10mL, disperse in boiling water bath.Add 2mol/L hydrochloric acid 21.5mL again, back hydrolysis 2h in boiling water bath, measure reducing sugar by GB/T5513-2008, be multiplied by 0.9 and namely obtain starch content.
After measured, the lotus seeds amylose starch of embodiment 3 extraction purification, in former state starch content for 88.5%, in butt starch content for 93.7%, crude protein wherein, ash content and lipid content three sums are less than 1%, purity is higher, can as the standard of lotus seeds amylose content determination method.
experimental example 3
The mensuration of lotus seeds amylose starch iodine binding capacity
The lotus seeds amylose starch 0.1000g of Example 3, comparative example 1, comparative example 7 and comparative example 8 extraction purification respectively, be added in 100mL volumetric flask, add several dehydrated alcohols moistening after, add 0.5mol/L sodium hydroxide solution 10mL again, put boiling water bath to disperse completely, the constant volume that adds water after cooling is to scale.Draw 5mL (being equivalent to containing amylose starch 5mg) dispersion liquid and, in 200mL beaker, add 85mL water, 1mol/L hydrochloric acid 5mL and 0.4mol/L liquor kalii iodide 5mL.Beaker is placed on magnetic stirrer, under platinum electrode and mercurous chloride electrode are inserted liquid level, with the titration of Potassium Iodate standardized solution, drips 0.1mL at every turn, add rear 1min and read millivolt number.Titration end point second derivative method calculates, and calculation formula is: amylose starch iodine binding capacity (%)=0.6346 × V × 100/m.
In formula: m-lotus seeds amylose starch quality, mg; V-8.34 × 10
-4mol/L potassium iodate solution titration volumes, mL; 0.6346-every milliliter 8.34 × 10
-4mol/L potassium iodate solution is equivalent to the iodine of 0.6346mg.
The iodine binding capacity measurement result of table 1 lotus seeds amylose starch
| Numbering | Iodine binding capacity (%) |
| Comparative example 1 | 9.90 |
| Comparative example 7 | 9.65 |
| Comparative example 8 | 10.15 |
| Embodiment 3 | 10.79 |
The measurement result of iodine binding capacity is as shown in table 1.As shown in Table 1, the iodine binding capacity of the lotus seeds amylose starch of embodiment 3 extraction purification is maximum, far above the iodine binding capacity of the lotus seeds amylose starch of institute's extraction purification in comparative example 1,7,8.This shows, the molecular structure impact of extracting and purifying method on lotus seeds amylose starch of embodiment 3 is minimum.
experimental example 4
The mensuration of lotus seeds amylose starch blue value
The lotus seeds amylose starch 0.1g of Example 3, comparative example 1, comparative example 7 and comparative example 8 extraction purification, makes the solution that concentration is 1mg/mL respectively.
Get the 1mg/mL lotus seeds amylose solution 1mL of above-mentioned preparation, add 35mL water, pH to 3.0 is regulated with the hydrochloric acid soln of 0.05mol/L, add 2% IKI 0.5mL, constant volume, to 50mL, fully mixes, and scans after 20min with spectrophotometer in 500nm-700nm, the wavelength at climax place is maximum absorption wavelength, and the absorbancy at this place is designated as Amax.
Calculation formula is as follows: the concentration of blue value=Amax × 4/ sample.In formula, the unit of the concentration of sample is mg/100mL.
The measurement result of the blue value of table 2 lotus seeds amylose starch
| Numbering | Blue value |
| Comparative example 7 | 0.798 |
| Comparative example 8 | 0.850 |
| Embodiment 3 | 0.888 |
| Comparative example 1 | 0.856 |
The blue value measurement result of lotus seeds amylose starch is as shown in table 2.As shown in Table 2, the blue value of the lotus seeds amylose starch of embodiment 3 extraction purification is maximum, far above the blue value of the lotus seeds amylose starch of institute's extraction purification in comparative example 1,7,8.This shows, the molecular structure impact of extracting and purifying method on lotus seeds amylose starch of embodiment 3 is minimum.
experimental example 5
The mensuration of lotus seeds amylose starch absorbancy
The each 0.1g of lotus seeds amylose starch of Example 3, comparative example 1, comparative example 2, comparative example 3, comparative example 4, comparative example 5, comparative example 6, comparative example 7 and comparative example 8 extraction purification respectively, after adding iodine colour developing, measure its absorbancy, result is as shown in table 3.
The measurement result of the absorbancy of table 3 lotus seeds amylose starch
| Numbering | Absorbancy |
| Comparative example 1 | 0.388 |
| Comparative example 2 | 0.499 |
| Embodiment 3 | 0.408 |
| Comparative example 3 | 0.344 |
| Comparative example 4 | 0.343 |
| Comparative example 5 | 0.340 |
| Comparative example 6 | 0.342 |
| Comparative example 7 | 0.367 |
| Comparative example 8 | 0.386 |
As shown in Table 3, the absorbancy of the lotus seeds amylose starch of embodiment 3 extraction purification is maximum.This shows, the molecular structure impact of extracting and purifying method on lotus seeds amylose starch of embodiment 3 is minimum.
The extracting and purifying method of lotus seeds amylose starch of the present invention; the lotus seed starch of gained is prepared for raw material with dry lotus seeds; by optimizing the concentration etc. of centrifugation rate and NaOH solution; under the prerequisite not destroying lotus seeds amylose molecule structure; improve efficiency, shorten the operating time, be conducive to the large-scale production of lotus seeds amylose starch; and the lotus seeds amylose starch purity high (about 94%) that institute's extraction purification obtains, can as the standard of lotus seeds amylose content determination method.
embodiment 1
The extracting and purifying method of a kind of lotus seeds amylose starch of the present embodiment, comprises the following steps:
A (), by after lotus seeds drying, pulverizes, cross 50 mesh sieves, obtain Semen Nelumbinis powder;
B () takes described Semen Nelumbinis powder 40g, add the 0.2mol/L sodium hydroxide solution of 0.1L, stirs, hold over night;
C the upper strata of reaction solution prepared by described step (b) discards by (), the water washing of lower sediment 0.08L 4 times, is colourless to upper solution;
D reaction solution that described step (c) is prepared by () carries out centrifugal, and centrifugal speed is 2000r/min, and centrifugation time is 15min, upper solution is discarded, and obtains throw out E;
E (), by described throw out E 0.04L washed with diethylether 4 times, obtains throw out F;
F () takes the described throw out F of 10g, add 0.05mol/L sodium hydroxide solution 0.06L, stirs, and 30 DEG C of heating 30min, discard upper solution;
Add water 0.04L in g reaction solution that () prepares to described step (f), centrifugal, centrifugal speed is 2000r/min, and centrifugation time is 15min, discards upper solution, obtains throw out G;
H described throw out G washes with water by (), the pH to upper solution is 7.0, obtains throw out H, and 30 DEG C of drying under reduced pressure, obtain described lotus seed starch;
(1) taking lotus seed starch 10g, add the sodium hydroxide solution of the 0.8mol/L of 0.30L, boiling water bath heated and stirred 15min, to disperseing completely, being cooled to room temperature;
(2) undertaken centrifugal by reaction solution prepared by described step (1), centrifugal speed is 4000r/min, and centrifugation time is 25min, gets supernatant liquor, and being adjusted to pH with 1.0mol/L hydrochloric acid is 6.0;
(3) the mixed solution 0.06L of propyl carbinol/primary isoamyl alcohol is added in the reaction solution prepared to step described in 0.32L (2), in the mixed solution of described propyl carbinol/primary isoamyl alcohol, the volume ratio of propyl carbinol and primary isoamyl alcohol is 1:1, boiling water bath heated and stirred 20min, be cooled to room temperature, 2 DEG C of standing 30h, discard upper solution, lower sediment is carried out centrifugal, centrifugal speed is 4000r/min, and centrifugation time is 15min, obtains throw out A;
(4) the saturated n-butanol aqueous solution of described throw out A is washed, centrifugal, centrifugal speed is 4000r/min, and centrifugation time is 15min, obtains sediment B;
(5) described sediment B is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred 10min, is cooled to room temperature, 2 DEG C of standing 30h, discard upper solution, are undertaken centrifugal by lower sediment, centrifugal speed is 4000r/min, and centrifugation time is 15min, obtains throw out C;
(6) described throw out C is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred, purifying 4 times, obtains sediment D repeatedly;
(7) by described sediment D washing with alcohol 2 times, freeze-drying is dry, obtains lotus seeds amylose starch.
embodiment 2
The extracting and purifying method of a kind of lotus seeds amylose starch of the present embodiment, comprises the following steps:
A (), by after lotus seeds drying, pulverizes, cross 300 mesh sieves, obtain Semen Nelumbinis powder;
B () takes described Semen Nelumbinis powder 40g, add the 0.05mol/L sodium hydroxide solution of 0.3L, stirs, hold over night;
C the upper strata of reaction solution prepared by described step (b) discards by (), the water washing of lower sediment 0.12L 2 times, is colourless to upper solution;
D reaction solution that described step (c) is prepared by () carries out centrifugal, and centrifugal speed is 4000r/min, and centrifugation time is 5min, upper solution is discarded, and obtains throw out E;
E (), by described throw out E 0.06L washed with diethylether 2 times, obtains throw out F;
F () takes the described throw out F of 10g, add 0.2mol/L sodium hydroxide solution 0.04L, stirs, and 70 DEG C of heating 10min, discard upper solution;
Add water 0.06L in g reaction solution that () prepares to described step (f), centrifugal, centrifugal speed is 4000r/min, and centrifugation time is 5min, discards upper solution, obtains throw out G;
H described throw out G washes with water by (), the pH to upper solution is 7.0, obtains throw out H, and 50 DEG C of drying under reduced pressure, obtain described lotus seed starch;
(1) taking lotus seed starch 10g, add the sodium hydroxide solution of the 0.5mol/L of 0.40L, boiling water bath heated and stirred 25min, to disperseing completely, being cooled to room temperature;
(2) undertaken centrifugal by reaction solution prepared by described step (1), centrifugal speed is 18000r/min, and centrifugation time is 15min, gets supernatant liquor, and being adjusted to pH with 2.0mol/L hydrochloric acid is 7.0;
(3) the mixed solution 0.10L of propyl carbinol/primary isoamyl alcohol is added in the reaction solution prepared to step described in 0.61L (2), in the mixed solution of described propyl carbinol/primary isoamyl alcohol, the volume ratio of propyl carbinol and primary isoamyl alcohol is 1:1, boiling water bath heated and stirred 10min, be cooled to room temperature, 4 DEG C of standing 20h, discard upper solution, lower sediment is carried out centrifugal, centrifugal speed is 18000r/min, and centrifugation time is 5min, obtains throw out A;
(4) the saturated n-butanol aqueous solution of described throw out A is washed, centrifugal, centrifugal speed is 18000r/min, and centrifugation time is 5min, obtains sediment B;
(5) described sediment B is placed in saturated n-butanol aqueous solution, heated and stirred 20min, is cooled to room temperature, 4 DEG C of standing 20h, discard upper solution, are undertaken centrifugal by lower sediment, centrifugal speed is 18000r/min, and centrifugation time is 5min, obtains throw out C;
(6) described throw out C is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred, purifying 6 times, obtains sediment D repeatedly;
(7) by described sediment D washing with alcohol 5 times, freeze-drying is dry, obtains lotus seeds amylose starch.
embodiment 3
The extracting and purifying method of a kind of lotus seeds amylose starch of the present embodiment, comprises the following steps:
A (), by after lotus seeds drying, pulverizes, cross 60 mesh sieves, obtain Semen Nelumbinis powder;
B () takes described Semen Nelumbinis powder 40g, add the 0.1mol/L sodium hydroxide solution of 0.2L, stirs, hold over night;
C the upper strata of reaction solution prepared by described step (b) discards by (), the water washing of lower sediment 0.1L 2 times, is colourless to upper solution;
D reaction solution that described step (c) is prepared by () carries out centrifugal, and centrifugal speed is 3000r/min, and centrifugation time is 10min, upper solution is discarded, and obtains throw out E;
E (), by described throw out E 0.05L washed with diethylether 3 times, obtains throw out F;
F () takes the described throw out F of 10g, add 0.1mol/L sodium hydroxide solution 0.05L, stirs, and 50 DEG C of heating 20min, discard upper solution;
Add water 0.05L in g reaction solution that () prepares to described step (f), centrifugal, centrifugal speed is 3000r/min, and centrifugation time is 10min, discards upper solution, obtains throw out G;
H described throw out G washes with water by (), the pH to upper solution is 7.0, obtains throw out H, and 40 DEG C of drying under reduced pressure, obtain described lotus seed starch;
(1) taking lotus seed starch 10g, add the sodium hydroxide solution of the 0.6mol/L of 0.35L, boiling water bath heated and stirred 20min, to disperseing completely, being cooled to room temperature;
(2) undertaken centrifugal by reaction solution prepared by described step (1), centrifugal speed is 7000r/min, and centrifugation time is 20min, gets supernatant liquor, and being adjusted to pH with 1.5mol/L hydrochloric acid is 6.5;
(3) the mixed solution 0.08L of propyl carbinol/primary isoamyl alcohol is added in the reaction solution prepared to step described in 0.46L (2), in the mixed solution of described propyl carbinol/primary isoamyl alcohol, the volume ratio of propyl carbinol and primary isoamyl alcohol is 1:1, boiling water bath heated and stirred 15min, be cooled to room temperature, 3 DEG C of standing 24h, discard upper solution, lower sediment is carried out centrifugal, centrifugal speed is 16000r/min, and centrifugation time is 10min, obtains throw out A;
(4) the saturated n-butanol aqueous solution of described throw out A is washed, centrifugal, centrifugal speed is 12000r/min, and centrifugation time is 5min, obtains sediment B;
(5) described sediment B is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred 15min, is cooled to room temperature, 3 DEG C of standing 24h, discard upper solution, are undertaken centrifugal by lower sediment, centrifugal speed is 16000r/min, and centrifugation time is 10min, obtains throw out C;
(6) described throw out C is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred, purifying 5 times, obtains sediment D repeatedly;
(7) by described sediment D washing with alcohol 4 times, freeze-drying is dry, obtains lotus seeds amylose starch.
embodiment 4
The extracting and purifying method of a kind of lotus seeds amylose starch of the present embodiment, comprises the following steps:
A (), by after lotus seeds drying, pulverizes, cross 60 mesh sieves, obtain Semen Nelumbinis powder;
B () takes described Semen Nelumbinis powder 40g, add the 0.1mol/L sodium hydroxide solution of 0.2L, stirs, hold over night;
C the upper strata of reaction solution prepared by described step (b) discards by (), the water washing of lower sediment 0.1L 2 times, is colourless to upper solution;
D reaction solution that described step (c) is prepared by () carries out centrifugal, and centrifugal speed is 3000r/min, and centrifugation time is 10min, upper solution is discarded, and obtains throw out E;
E (), by described throw out E 0.05L washed with diethylether 2 times, obtains throw out F;
F () takes the described throw out F of 10g, add 0.1mol/L sodium hydroxide solution 0.05L, stirs, and 50 DEG C of heating 20min, discard upper solution;
Add water 0.05L in g reaction solution that () prepares to described step (f), centrifugal, centrifugal speed is 3000r/min, and centrifugation time is 10min, discards upper solution, obtains throw out G;
H described throw out G washes with water by (), the pH to upper solution is 7.0, obtains throw out H, and 40 DEG C of drying under reduced pressure, obtain described lotus seed starch;
(1) taking lotus seed starch 10g, add the sodium hydroxide solution of the 0.6mol/L of 0.35L, boiling water bath heated and stirred 20min, to disperseing completely, being cooled to room temperature;
(2) undertaken centrifugal by reaction solution prepared by described step (1), centrifugal speed is 18000r/min, and centrifugation time is 20min, gets supernatant liquor, and being adjusted to pH with 1.5mol/L hydrochloric acid is 6.5;
(3) the mixed solution 0.08L of propyl carbinol/primary isoamyl alcohol is added in the reaction solution prepared to step described in 0.46L (2), in the mixed solution of described propyl carbinol/primary isoamyl alcohol, the volume ratio of propyl carbinol and primary isoamyl alcohol is 1:1, boiling water bath heated and stirred 15min, be cooled to room temperature, 3 DEG C of standing 24h, discard upper solution, lower sediment is carried out centrifugal, centrifugal speed is 18000r/min, and centrifugation time is 10min, obtains throw out A;
(4) the saturated n-butanol aqueous solution of described throw out A is washed, centrifugal, centrifugal speed is 18000r/min, and centrifugation time is 5min, obtains sediment B;
(5) described sediment B is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred 15min, is cooled to room temperature, 3 DEG C of standing 24h, discard upper solution, are undertaken centrifugal by lower sediment, centrifugal speed is 18000r/min, and centrifugation time is 10min, obtains throw out C;
(6) described throw out C is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred, purifying 5 times, obtains sediment D repeatedly;
(7) by described sediment D washing with alcohol 4 times, freeze-drying is dry, obtains lotus seeds amylose starch.
embodiment 5
The extracting and purifying method of a kind of lotus seeds amylose starch of the present embodiment, comprises the following steps:
A (), by after lotus seeds drying, pulverizes, cross 60 mesh sieves, obtain Semen Nelumbinis powder;
B () takes described Semen Nelumbinis powder 40g, add the 0.1mol/L sodium hydroxide solution of 0.2L, stirs, hold over night;
C the upper strata of reaction solution prepared by described step (b) discards by (), the water washing of lower sediment 0.1L 2 times, is colourless to upper solution;
D reaction solution that described step (c) is prepared by () carries out centrifugal, and centrifugal speed is 3000r/min, and centrifugation time is 10min, upper solution is discarded, and obtains throw out E;
E (), by described throw out E 0.05L washed with diethylether 2 times, obtains throw out F;
F () takes the described throw out F of 10g, add 0.1mol/L sodium hydroxide solution 0.05L, stirs, and 50 DEG C of heating 20min, discard upper solution;
Add water 0.05L in g reaction solution that () prepares to described step (f), centrifugal, centrifugal speed is 3000r/min, and centrifugation time is 10min, discards upper solution, obtains throw out G;
H described throw out G washes with water by (), the pH to upper solution is 7.0, obtains throw out H, and 40 DEG C of drying under reduced pressure, obtain described lotus seed starch;
(1) taking lotus seed starch 10g, add the sodium hydroxide solution of the 0.6mol/L of 0.35L, boiling water bath heated and stirred 20min, to disperseing completely, being cooled to room temperature;
(2) undertaken centrifugal by reaction solution prepared by described step (1), centrifugal speed is 10000r/min, and centrifugation time is 20min, gets supernatant liquor, and being adjusted to pH with 1.5mol/L hydrochloric acid is 6.5;
(3) the mixed solution 0.08L of propyl carbinol/primary isoamyl alcohol is added in the reaction solution prepared to step described in 0.46L (2), in the mixed solution of described propyl carbinol/primary isoamyl alcohol, the volume ratio of propyl carbinol and primary isoamyl alcohol is 1:1, boiling water bath heated and stirred 15min, be cooled to room temperature, 3 DEG C of standing 24h, discard upper solution, lower sediment is carried out centrifugal, centrifugal speed is 10000r/min, and centrifugation time is 10min, obtains throw out A;
(4) the saturated n-butanol aqueous solution of described throw out A is washed, centrifugal, centrifugal speed is 10000r/min, and centrifugation time is 5min, obtains sediment B;
(5) described sediment B is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred 15min, is cooled to room temperature, 3 DEG C of standing 24h, discard upper solution, are undertaken centrifugal by lower sediment, centrifugal speed is 10000r/min, and centrifugation time is 10min, obtains throw out C;
(6) described throw out C is placed in saturated n-butanol aqueous solution, boiling water bath heated and stirred, purifying 5 times, obtains sediment D repeatedly;
(7) by described sediment D washing with alcohol 4 times, freeze-drying is dry, obtains lotus seeds amylose starch.
comparative example 1
The extracting and purifying method of the present embodiment lotus seeds amylose starch is all identical with the operating parameters of each step of the extracting and purifying method of embodiment 3 lotus seeds amylose starch, and difference is only: in step (1), the concentration of sodium hydroxide solution is 0.7mol/L.
comparative example 2
The extracting and purifying method of the present embodiment lotus seeds amylose starch is all identical with the operating parameters of each step of the extracting and purifying method of embodiment 3 lotus seeds amylose starch, and difference is only: adopt boulton process dry in step (7).
comparative example 3
The extracting and purifying method of the present embodiment lotus seeds amylose starch is all identical with the operating parameters of each step of the extracting and purifying method of embodiment 3 lotus seeds amylose starch, difference is only: in step (2), step (3), step (4) and step (5), centrifugal speed is 3000r/min.
comparative example 4
The extracting and purifying method of the present embodiment lotus seeds amylose starch is all identical with the operating parameters of each step of the extracting and purifying method of embodiment 3 lotus seeds amylose starch, difference is only: in step (2), step (3), step (4) and step (5), centrifugal speed is 4000r/min.
comparative example 5
The extracting and purifying method of the present embodiment lotus seeds amylose starch is all identical with the operating parameters of each step of the extracting and purifying method of embodiment 3 lotus seeds amylose starch, difference is only: in step (2), step (3), step (4) and step (5), centrifugal speed is 5000r/min.
comparative example 6
The extracting and purifying method of the present embodiment lotus seeds amylose starch is all identical with the operating parameters of each step of the extracting and purifying method of embodiment 3 lotus seeds amylose starch, difference is only: in step (2), step (3), step (4) and step (5), centrifugal speed is 16000r/min.
comparative example 7
The extracting and purifying method of the present embodiment lotus seeds amylose starch is all identical with the operating parameters of each step of the extracting and purifying method of embodiment 3 lotus seeds amylose starch, and difference is only: in step (1), the concentration of sodium hydroxide solution is 0.4mol/L.
comparative example 8
The extracting and purifying method of the present embodiment lotus seeds amylose starch is all identical with the operating parameters of each step of the extracting and purifying method of embodiment 3 lotus seeds amylose starch, and difference is only: in step (1), the concentration of sodium hydroxide solution is 0.5mol/L.
Obviously, above-described embodiment is only for clearly example being described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still among the protection domain of the invention.
Claims (10)
1. an extracting and purifying method for lotus seeds amylose starch, is characterized in that, comprises the following steps:
(1) taking lotus seed starch 10 weight part, add the sodium hydroxide solution of 0.30-0.40 parts by volume 0.5-0.8mol/L, heated and stirred 15-25min, to disperseing completely, being cooled to room temperature;
(2) undertaken centrifugal by reaction solution prepared by described step (1), centrifugal speed is 4000-18000r/min, and centrifugation time is 15-25min, gets supernatant liquor, and being adjusted to pH with 1.0-2.0mol/L hydrochloric acid is 6.0-7.0;
(3) the mixed solution 0.06-0.10 parts by volume of propyl carbinol/primary isoamyl alcohol is added in the reaction solution prepared to described step (2), heated and stirred 10-20min, be cooled to room temperature, 2-4 DEG C of standing 20-30h, upper solution discarded, undertaken centrifugal by lower sediment, centrifugal speed is 4000-18000r/min, centrifugation time is 5-15min, obtains throw out A;
(4) the saturated n-butanol aqueous solution of described throw out A is washed, centrifugal, centrifugal speed is 4000-18000r/min, and centrifugation time is 5-15min, obtains sediment B;
(5) described sediment B is placed in saturated n-butanol aqueous solution, heated and stirred 10-20min, is cooled to room temperature, 2-4 DEG C of standing 20-30h, discards upper solution, is undertaken centrifugal by lower sediment, centrifugal speed is 4000-18000r/min, and centrifugation time is 5-15min, obtains throw out C;
(6) described throw out C is placed in saturated n-butanol aqueous solution, heated and stirred, purifying 4-6 time, obtains sediment D repeatedly;
(7) by described sediment D washing with alcohol 2-5 time, dry, obtain lotus seeds amylose starch;
The pass of described weight part and parts by volume is g/L.
2. the extracting and purifying method of lotus seeds amylose starch according to claim 1, it is characterized in that, in at least one step of described step (2), step (3), step (4) and step (5), described centrifugal speed is 10000-18000r/min.
3. the extracting and purifying method of lotus seeds amylose starch according to claim 1 and 2, is characterized in that, in described step (1), the concentration of described sodium hydroxide solution is 0.6mol/L or 0.7mol/L.
4. the extracting and purifying method of the lotus seeds amylose starch according to any one of claim 1-3, it is characterized in that, in described step (1), step (3), step (5) and step (6), described in be heated to be boiling water bath heating.
5. the extracting and purifying method of the lotus seeds amylose starch according to any one of claim 1-4, is characterized in that, in described step (3) and step (5), the described standing time is 24h.
6. the extracting and purifying method of the lotus seeds amylose starch according to any one of claim 1-5, it is characterized in that, in described step (3), in the mixed solution of described propyl carbinol/primary isoamyl alcohol, the volume ratio of propyl carbinol and primary isoamyl alcohol is 1:1, and the volume ratio of reaction solution prepared by the mixed solution of described propyl carbinol/primary isoamyl alcohol and described step (2) is 1:5.25-6.125.
7. the extracting and purifying method of the lotus seeds amylose starch according to any one of claim 1-6, is characterized in that, in described step (7), described drying adopts freeze-drying or boulton process.
8. the extracting and purifying method of the lotus seeds amylose starch according to any one of claim 1-7, is characterized in that, in described step (1), described lotus seed starch obtains as follows:
A (), by after lotus seeds drying, pulverizes, cross 50-300 mesh sieve, obtain Semen Nelumbinis powder;
B () takes described Semen Nelumbinis powder 40 weight part, add 0.1-0.3 parts by volume 0.05-0.2mol/L sodium hydroxide solution, stirs, hold over night;
C the upper strata of reaction solution prepared by described step (b) discards by (), lower sediment water washing 2-4 time of 0.08-0.12 parts by volume is colourless to upper solution;
D reaction solution that described step (c) is prepared by () carries out centrifugal, and centrifugal speed is 2000-4000r/min, and centrifugation time is 5-15min, upper solution is discarded, and obtains throw out E;
E (), by described throw out E 0.04-0.06 parts by volume washed with diethylether 2-4 time, obtains throw out F;
F () takes the described throw out F of 10 weight parts, add 0.05-0.2mol/L sodium hydroxide solution 0.04-0.06 parts by volume, stirs, and 30-70 DEG C of heating 10-30min, discards upper solution;
Add water 0.04-0.06 parts by volume in g reaction solution that () prepares to described step (f), centrifugal, centrifugal speed is 2000-4000r/min, and centrifugation time is 5-15min, discards upper solution, obtains throw out G;
H described throw out G washes with water by (), the pH to upper solution is 7.0, obtains throw out H, and 30-50 DEG C of drying, obtains described lotus seed starch;
The pass of described weight part and parts by volume is g/L.
9. the extracting and purifying method of lotus seeds amylose starch according to claim 8, is characterized in that, described step (b) is with in step (f), and the concentration of described sodium hydroxide solution is 0.1mol/L.
10. the extracting and purifying method of lotus seeds amylose starch according to claim 8 or claim 9, is characterized in that, in described step (d) and/or described step (g), described centrifugal speed is 3000r/min; In described step (h), described drying is drying under reduced pressure.
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| CN109651515A (en) * | 2018-12-10 | 2019-04-19 | 北京工商大学 | A method of processing lotus seeds scarlet reground grain |
| CN114989316A (en) * | 2022-06-01 | 2022-09-02 | 山东省农业科学院 | Method for obtaining high amylose starch from cashew kernels |
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| CN101518343A (en) * | 2009-04-02 | 2009-09-02 | 福建农林大学 | Method for preparing lotus seed starch sponge |
| CN104628873A (en) * | 2015-02-10 | 2015-05-20 | 天津大学 | Method for separating high-solubility pueraria starch from pueraria powder |
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| CN101518343A (en) * | 2009-04-02 | 2009-09-02 | 福建农林大学 | Method for preparing lotus seed starch sponge |
| CN104628873A (en) * | 2015-02-10 | 2015-05-20 | 天津大学 | Method for separating high-solubility pueraria starch from pueraria powder |
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| CN109651515A (en) * | 2018-12-10 | 2019-04-19 | 北京工商大学 | A method of processing lotus seeds scarlet reground grain |
| CN114989316A (en) * | 2022-06-01 | 2022-09-02 | 山东省农业科学院 | Method for obtaining high amylose starch from cashew kernels |
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