CN109134695B - Alcohol-soluble beta-glucan extract, preparation method and application thereof, and health-care wine - Google Patents
Alcohol-soluble beta-glucan extract, preparation method and application thereof, and health-care wine Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
Abstract
An alcohol-soluble beta-glucan extract, a preparation method and application thereof, and health care wine, and relates to the technical field of extraction of plant components. The alcohol-soluble beta-glucan extract is prepared by adding acid solution into highland barley, and performing acid extraction; adding alpha-amylase and glucoamylase into the acid extract, carrying out enzymolysis, and carrying out enzyme deactivation treatment to obtain an enzymolysis extract; centrifuging and concentrating the enzymolysis extracting solution to obtain a concentrated solution; adding an ethanol solution into the concentrated solution, and carrying out ethanol precipitation to obtain an ethanol precipitation solution; the alcohol precipitation liquid is filtered and dried, and the process is simple, convenient to operate, short in production period, high in efficiency and suitable for industrial mass production; the prepared alcohol-soluble beta-glucan extract has good alcohol solubility, can be dissolved in ethanol with different concentrations, and is suitable for producing highland barley beta-glucan products; the application of the alcohol-soluble beta-glucan extract is used for preparing health care wine; the health wine contains a large amount of highland barley beta-glucan.
Description
Technical Field
The invention relates to the technical field of extraction of plant components, and particularly relates to an alcohol-soluble beta-glucan extract, a preparation method and application thereof, and health-care wine.
Background
Highland barley (Hordeum vulgar L.var. nudum hook. f.) belongs to the barley crop of the Gramineae family, and because the seeds of the highland barley have no shells, the highland barley, the naked barley, the rice barley, the Huai wheat and the Yuanmai are also called as naked barley, the rice barley, the Huai wheat and the Yuanmai and are mainly distributed in Qinghai, Tibet, Sichuan Ganzui, Yunnan Diqing, Gansu Ganna and the like in China. The highland barley is the only crop in the alpine region with the altitude of 4200-4500m, has the characteristics of high protein, high fiber, high vitamin, low fat, low sugar and the like, and is rich in trace elements, beta-glucan and the like.
The highland barley contains more beta-glucan, and researches show that the beta-glucan has the effects of clearing intestines, reducing blood fat, cholesterol, regulating blood sugar, preventing cardiovascular diseases, improving immunity, resisting tumors and the like, and is a soluble dietary fiber which is very beneficial to human health. The chemical name of beta-glucan is: (1-3) (1-4) -beta-D-glucan, a class of non-starch polysaccharides that are poorly water soluble and ethanol insoluble, and the solubility of beta-glucan limits its use in liquid products, particularly alcoholic products. Therefore, the extract containing the highland barley beta-glucan is extracted under proper extraction conditions, the solubility of the extract is improved, and the method has important significance for the development and utilization of the highland barley.
At present, the extraction method of highland barley beta-glucan mainly comprises a hot water method, an acid extraction method and an alkali extraction method. The beta-glucan extracted by the hot water method has high purity, but low extraction amount, long extraction time and high production cost, and is not suitable for industrial production; the acid extraction method easily causes excessive hydrolysis of the cereal starch, and a large amount of glucose can be mixed into a beta-glucan product, so that the product purity is low, and the separation process is difficult. The alkali extraction hydrolysis can hydrolyze part of seed coat cellulose, can enable more beta-glucan combined with the cellulose to be dissociated into an aqueous solution, increases the yield of the beta-glucan, but needs long-time extraction, and has long production period. Moreover, the highland barley beta-glucan prepared by the prior art is insoluble in ethanol, so that the application range of the beta-glucan is limited.
Therefore, a method which has simple working procedures and short production period and can extract the highland barley extract containing the beta-glucan and having good alcohol solubility is needed.
Disclosure of Invention
The invention aims to provide a preparation method of an alcohol-soluble beta-glucan extract, which has the advantages of simple process, convenient operation, short production period and high efficiency and is suitable for industrial mass production.
The invention also aims to provide an alcohol-soluble beta-glucan extract which has good alcohol solubility, can be dissolved in ethanol with different concentrations and is suitable for producing highland barley beta-glucan products.
The invention also aims to provide application of the alcohol-soluble beta-glucan extract in preparing health-care wine.
The invention also aims to provide health care wine which contains a large amount of highland barley beta-glucan.
The technical problem to be solved by the invention is realized by adopting the following technical scheme.
The invention provides a preparation method of an alcohol-soluble beta-glucan extract, which comprises the following steps:
taking highland barley, adding an acid solution, and performing acid extraction at 40-60 ℃ to obtain an acid extracting solution;
adding alpha-amylase accounting for 0.05-0.2% of the weight of the acid extract and saccharifying enzyme accounting for 0.2-0.6% of the weight of the acid extract into the acid extract, carrying out enzymolysis at 40-60 ℃, and carrying out enzyme deactivation treatment after enzymolysis is finished to obtain an enzymolysis extract;
centrifuging and concentrating the enzymolysis extracting solution to obtain a concentrated solution;
adding an ethanol solution into the concentrated solution, and carrying out ethanol precipitation to obtain an ethanol precipitation solution;
filtering the alcohol precipitation solution, and drying.
Further, in a preferred embodiment of the present invention, the method of adding the acid solution is: adding water which is 15-25 times of the weight of the highland barley into the highland barley medicinal material, and adding citric acid to adjust the pH value to 4-6.
Further, in the preferred embodiment of the present invention, the acid extraction method is: extracting for 2-3 times at 40-60 ℃ for 1-2 hours each time.
Further, in a preferred embodiment of the present invention, the enzymolysis time is 1 to 2 hours.
Further, in a preferred embodiment of the present invention, the enzyme deactivation method comprises: heating to 80-90 ℃ and preserving the heat for 40-60 minutes.
Further, in the preferred embodiment of the present invention, the concentration is to concentrate the clear liquid obtained by centrifugation to 1-3 times of the weight of the highland barley medicinal material.
Further, in a preferred embodiment of the present invention, the alcohol precipitation method comprises: adding 90-95% ethanol into the concentrated solution, and controlling the ethanol concentration in the ethanol precipitation solution to be 50-70%.
The invention provides an alcohol-soluble beta-glucan extract, which is prepared by adopting the preparation method of the alcohol-soluble beta-glucan extract.
The invention provides an application of the alcohol-soluble beta-glucan extract, and the alcohol-soluble beta-glucan extract is used for preparing health care wine.
The invention provides health wine, which comprises white spirit with the alcoholic strength of 40-60% vol and an alcohol-soluble beta-glucan extract as claimed in claim 8, wherein the addition amount of the alcohol-soluble beta-glucan extract is 1-8% of the mass of the white spirit.
The alcohol-soluble beta-glucan extract, the preparation method and the application thereof of the embodiment of the invention, and the health wine have the beneficial effects that: the preparation method of the alcohol-soluble beta-glucan extract provided by the embodiment of the invention comprises the steps of taking highland barley medicinal materials, adding an acid solution, and carrying out acid extraction at 40-60 ℃ to obtain an acid extracting solution; adding alpha-amylase accounting for 0.05-0.2% of the weight of the acid extract and saccharifying enzyme accounting for 0.2-0.6% of the weight of the acid extract into the acid extract, carrying out enzymolysis at 40-60 ℃, and carrying out enzyme deactivation treatment after enzymolysis is finished to obtain an enzymolysis extract; centrifuging and concentrating the enzymolysis extracting solution to obtain a concentrated solution; adding an ethanol solution into the concentrated solution, and carrying out ethanol precipitation to obtain an ethanol precipitation solution; the alcohol precipitation liquid is filtered and dried, and the process is simple, convenient to operate, short in production period, high in efficiency and suitable for industrial mass production; the prepared alcohol-soluble beta-glucan extract has good alcohol solubility, can be dissolved in ethanol with different concentrations, and is suitable for producing highland barley beta-glucan products; the alcohol-soluble beta-glucan extract provided by the embodiment of the invention is used for preparing health care wine; the health care wine provided by the embodiment of the invention contains a large amount of highland barley beta-glucan.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a process flow chart of a method for preparing an alcohol-soluble β -glucan extract according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The alcohol-soluble beta-glucan extract, the preparation method and the application thereof, and the health wine of the embodiment of the invention are specifically described below.
The invention aims to provide a preparation method of an alcohol-soluble beta-glucan extract, which comprises the following steps:
(1) acid extraction: taking highland barley, adding an acid solution, and performing acid extraction at 40-60 ℃ to obtain an acid extracting solution. In this example, the method of adding the acid solution is generally: adding water in an amount which is 15-25 times the weight of the highland barley medicinal material into the highland barley medicinal material, and adding citric acid to adjust the pH value to 4-6; the acid extraction method generally comprises: extracting for 2-3 times at 40-60 ℃ for 1-2 hours each time.
(2) Enzymolysis: adding alpha-amylase accounting for 0.05-0.2% of the weight of the acid extract and saccharifying enzyme accounting for 0.2-0.6% of the weight of the acid extract into the acid extract, carrying out enzymolysis at 40-60 ℃, wherein the enzymolysis time is generally 1-2 hours, and carrying out enzyme deactivation treatment after the enzymolysis is finished, wherein the enzyme deactivation method generally comprises the following steps: heating to 80-90 ℃ and preserving the heat for 40-60 minutes to obtain an enzymolysis extracting solution;
(3) centrifuging: and centrifuging the enzymolysis extracting solution to obtain clear liquid. The method of centrifugation is generally: through one or two of horizontal spiral shell centrifuge, disk centrifuge, this embodiment is to cross horizontal spiral shell centrifuge, disk centrifuge with the enzymolysis extract in proper order.
(4) Concentration: and concentrating the clear liquid obtained by centrifugation to 1-3 times of the mass of the highland barley medicinal material to obtain a concentrated solution.
(5) Alcohol precipitation: and adding an ethanol solution into the concentrated solution, and carrying out ethanol precipitation to obtain an ethanol precipitation solution. In this embodiment, the alcohol precipitation method generally includes: adding 90-95% ethanol into the concentrated solution, and controlling the ethanol concentration in the ethanol precipitation solution to be 50-70%.
(6) And (3) filtering: filtering the alcohol precipitation solution, and collecting the alcohol precipitation precipitate.
(7) And (3) drying: drying the alcohol precipitation at 40-60 deg.c to obtain alcohol soluble beta-glucan extract.
The preparation method of the alcohol-soluble beta-glucan extract of the present example has the following advantages:
1. simple process, short production period, low cost and easy popularization. Compared with the common highland barley beta-glucan extraction process, the method has the advantages that the acidic solvent is utilized to completely extract glucan, the advantages of short enzyme action time and high efficiency are utilized, the beta-glucan wrapped in highland barley starch is dissociated into the aqueous solution, the beta-glucan in highland barley is completely extracted, and the production period can be shortened and the production cost can be reduced by combining the advantages of the beta-glucan and the aqueous solution.
2. Mild condition and environmental protection: at present, no matter acid extraction or alkali extraction is adopted, wastewater generated in the production process can generate certain pollution. The embodiment adopts a method combining acid extraction and enzymolysis, neutralizes acidic substances by utilizing self reaction, reduces the pollution of wastewater to the minimum, has mild extraction conditions, does not have drastic temperature difference change among all working procedures, reduces energy waste, and is beneficial to further reducing cost and saving resources.
3. Improve the alcohol solubility and enlarge the application range: the highland barley beta-glucan is difficult to dissolve in ethanol due to the molecular structure characteristics, so that the application range of the highland barley beta-glucan is limited. In the embodiment, the beta-glucan in the highland barley is extracted by an acid extraction enzymolysis method, and the highland barley beta-glucan extract with good alcohol solubility is obtained by technologies such as combined separation, alcohol precipitation and the like, and specifically, the molecular weight of the beta-glucan extract is controlled within a proper range, so that the alcohol solubility of the beta-glucan extract is improved, and the beta-glucan extract can be applied to ethanol-containing products.
4. The raw materials are fully utilized: according to the conventional highland barley beta-glucan extraction process, the extraction residues cannot be further utilized, waste is caused, the extraction residues obtained in the embodiment have low water content and are easy to dry, and the extraction residues can be used as raw materials for producing crude fiber products or brewing wine, so that the resource utilization maximization is realized.
The invention aims to provide an alcohol-soluble beta-glucan extract, which is prepared by adopting the preparation method of the alcohol-soluble beta-glucan extract. The alcohol-soluble beta-glucan extract has good alcohol solubility and is easy to dissolve in an ethanol solution; the alcohol-soluble beta-glucan extract has high content of beta-glucan, and the mass content of the beta-glucan reaches 28-35%.
The invention aims to provide the application of the alcohol-soluble beta-glucan extract, and the alcohol-soluble beta-glucan extract is used for preparing health care wine.
The invention aims to provide health-care wine which comprises white spirit with the alcoholic strength of 40-60% vol and the alcohol-soluble beta-glucan extract, wherein the addition amount of the alcohol-soluble beta-glucan extract is 1-8% of the mass of the white spirit. Generally, white spirit with the alcoholic strength of 40-60 vol% is taken, alcohol-soluble beta-glucan extract is added, the mixture is uniformly stirred, and the mixture is filtered after standing for 5-30 days, so that the health-care wine is obtained.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
This example provides an alcohol-soluble β -glucan extract, which is obtained by the following preparation method:
(1) extraction: extracting 20kg of highland barley with acid solution for 2 times, adding 300L of water each time, adding citric acid to adjust pH to 4, extracting at 40 deg.C for 2 hr, and mixing the two extractive solutions to obtain acid extractive solution.
(2) Enzymolysis: adding alpha-amylase accounting for 0.05 percent of the weight of the acid extract and 0.2 percent of saccharifying enzyme into the acid extract, carrying out enzymolysis for 1 hour at 40 ℃, heating to 80 ℃ after the enzymolysis is finished, and keeping the temperature for 40 minutes to inactivate the enzyme to obtain an enzymolysis extract.
(3) Centrifuging: sequentially passing the enzymolysis extractive solution through a horizontal screw centrifuge and a disc centrifuge, and collecting the clear liquid.
(4) Concentration: concentrating the clear liquid to 1 time of the weight of the highland barley medicinal material to obtain concentrated solution.
(5) Alcohol precipitation: adding 95% ethanol into the concentrated solution, and controlling the ethanol concentration at 50% to obtain ethanol precipitation solution.
(6) And (3) filtering: filtering the alcohol precipitation solution, and collecting the alcohol precipitation precipitate.
(7) And (3) drying: drying the precipitate to obtain 2.43kg of alcohol soluble beta-glucan extract. The detection proves that the content of the beta-glucan in the product is 28.34%.
The embodiment also provides health wine, which is prepared by taking 50kg of white spirit with the alcoholic strength of 50% vol, adding 1kg of alcohol-soluble beta-glucan extract, uniformly stirring, completely dissolving the alcohol-soluble beta-glucan extract in the white spirit, standing for 10 days, allowing a small amount of precipitate to exist at the bottom of a wine body, and filtering.
Example 2
This example provides an alcohol-soluble β -glucan extract, which is obtained by the following preparation method:
(1) extraction: extracting 20kg of highland barley with acid solution for 2 times, adding 500L of water each time, adding citric acid to adjust pH to 6, extracting at 60 deg.C for 3 times, each time for 2 hr, and mixing the two extractive solutions to obtain acid extractive solution.
(2) Enzymolysis: adding alpha-amylase accounting for 0.2% of the mass of the acid extracting solution and 0.6% of saccharifying enzyme into the acid extracting solution, carrying out enzymolysis for 2 hours at 60 ℃, heating to 90 ℃ after the enzymolysis is finished, keeping the temperature for 50 minutes, and inactivating the enzyme to obtain an enzymolysis extracting solution.
(3) Centrifuging: sequentially passing the enzymolysis extractive solution through a horizontal screw centrifuge and a disc centrifuge, and collecting the clear liquid.
(4) Concentration: concentrating the centrifugal clear liquid to 3 times of the weight of the highland barley medicinal material to obtain concentrated solution.
(5) Alcohol precipitation: adding ethanol into the concentrated solution, and controlling the concentration of the ethanol at 70% to obtain an ethanol precipitation solution.
(6) And (3) filtering: filtering the alcohol precipitation solution, and collecting the alcohol precipitation precipitate.
(7) And (3) drying: drying the precipitate to obtain 2.68kg of alcohol soluble beta-glucan extract. The detection proves that the content of the beta-glucan in the product is 30.93 percent.
The embodiment also provides health wine, which is prepared by taking 50kg of white spirit with the alcoholic strength of 50 vol%, adding 2kg of alcohol-soluble beta-glucan extract, uniformly stirring, completely dissolving the alcohol-soluble beta-glucan extract in the white spirit, standing for 10 days, allowing a small amount of precipitate to exist at the bottom of a wine body, and filtering.
Example 3
This example provides an alcohol-soluble β -glucan extract, which is obtained by the following preparation method:
(1) extraction: taking 40kg of highland barley medicinal material, adding 800L of water, adding citric acid to adjust pH to 5, extracting at 50 ℃ for 2 times, each time for 2 hours, and combining the two extracting solutions to obtain an acid extracting solution.
(2) Enzymolysis: adding alpha-amylase accounting for 0.1% of the weight of the acid extract and 0.4% of saccharifying enzyme into the acid extract, carrying out enzymolysis for 2 hours at 50 ℃, heating to 90 ℃ after the enzymolysis is finished, keeping the temperature for 30 minutes, and inactivating the enzyme to obtain an enzymolysis extract.
(3) Centrifuging: sequentially passing the enzymolysis extractive solution through a horizontal screw centrifuge and a disc centrifuge, and collecting the clear liquid.
(4) Concentration: concentrating the centrifugal clear liquid to 2 times of the weight of the highland barley medicinal material to obtain concentrated solution.
(5) Alcohol precipitation: adding ethanol into the concentrated solution, and controlling the concentration of the ethanol to be 65% to obtain an ethanol precipitation solution.
(6) And (3) filtering: filtering the alcohol precipitation solution, and collecting the alcohol precipitation precipitate.
(7) And (3) drying: drying the alcohol precipitation to obtain 2.57kg of alcohol-soluble beta-glucan extract. The detection proves that the content of the beta-glucan is 32.74%.
The embodiment also provides health wine, which is prepared by taking 50kg of white spirit with the alcoholic strength of 50% vol, adding 2.5kg of alcohol-soluble beta-glucan extract, uniformly stirring, completely dissolving the alcohol-soluble beta-glucan extract in the white spirit, standing for 10 days, only allowing a small amount of precipitate to exist at the bottom of a wine body, and filtering.
Example 4
This example provides an alcohol-soluble β -glucan extract, which is obtained by the following preparation method:
(1) extraction: adding water 450L into 30kg of highland barley, adding citric acid to adjust pH to 4, extracting at 60 deg.C for 1 hr for 3 times, and mixing the two extractive solutions to obtain acid extractive solution.
(2) Enzymolysis: adding alpha-amylase accounting for 0.05 percent of the weight of the acid extract and 0.6 percent of saccharifying enzyme into the acid extract, carrying out enzymolysis for 1 hour at 40 ℃, heating to 80 ℃ after the enzymolysis is finished, and keeping the temperature for 60 minutes to inactivate the enzyme to obtain an enzymolysis extract.
(3) Centrifuging: sequentially passing the enzymolysis extractive solution through a horizontal screw centrifuge and a disc centrifuge, and collecting the clear liquid.
(4) Concentration: concentrating the centrifugal clear liquid to 3 times of the weight of the highland barley medicinal material to obtain concentrated solution.
(5) Alcohol precipitation: adding ethanol into the concentrated solution, and controlling the concentration of the ethanol at 70% to obtain an ethanol precipitation solution.
(6) And (3) filtering: filtering the alcohol precipitation solution, and collecting the alcohol precipitation precipitate.
(7) And (3) drying: drying the precipitate to obtain 2.39kg of alcohol soluble beta-glucan extract. The detection proves that the content of the beta-glucan in the product is 30.64%.
The embodiment also provides health wine, which is prepared by taking 50kg of white spirit with the alcoholic strength of 50% vol, adding 3.5kg of alcohol-soluble beta-glucan extract, uniformly stirring, completely dissolving the alcohol-soluble beta-glucan extract in the white spirit, standing for 15 days, only allowing a small amount of precipitate to exist at the bottom of a wine body, and filtering.
In conclusion, the preparation method of the alcohol-soluble beta-glucan extract provided by the embodiment of the invention has the advantages of simple process, convenience in operation, short production period and high efficiency, and is suitable for industrial mass production; the prepared alcohol-soluble beta-glucan extract has good alcohol solubility, can be dissolved in ethanol with different concentrations, and is suitable for producing highland barley beta-glucan products; the alcohol-soluble beta-glucan extract provided by the embodiment of the invention is used for preparing health care wine; the health care wine provided by the embodiment of the invention contains a large amount of highland barley beta-glucan.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (5)
1. A method for preparing an alcohol-soluble beta-glucan extract, characterized by comprising the steps of:
taking highland barley, adding an acid solution, and performing acid extraction at 40-60 ℃ to obtain an acid extracting solution;
adding alpha-amylase accounting for 0.05-0.2% of the mass of the acid extracting solution and saccharifying enzyme accounting for 0.2-0.6% of the mass of the acid extracting solution into the acid extracting solution, carrying out enzymolysis at 40-60 ℃, and carrying out enzyme deactivation treatment after enzymolysis is finished to obtain an enzymolysis extracting solution;
centrifuging and concentrating the enzymolysis extracting solution to obtain a concentrated solution;
adding an ethanol solution into the concentrated solution, and carrying out ethanol precipitation to obtain an ethanol precipitation solution;
filtering and drying the alcohol precipitation solution;
the method for adding the acid solution comprises the following steps: adding water in an amount which is 15-25 times the weight of the highland barley medicinal material into the highland barley medicinal material, and adding citric acid to adjust the pH value to 4-6;
the acid extraction method comprises the following steps: extracting for 2-3 times at 40-60 ℃ for 1-2 hours each time;
the enzymolysis time is 1-2 hours;
the enzyme deactivation method comprises the following steps: heating to 80-90 ℃ and preserving the heat for 40-60 minutes;
the method for alcohol precipitation comprises the following steps: adding 90-95% ethanol by mass concentration into the concentrated solution, and controlling the ethanol concentration in the ethanol precipitation solution to be 50-70%.
2. The method for preparing an alcohol-soluble β -glucan extract as claimed in claim 1, wherein the concentration is performed by concentrating the supernatant obtained by centrifugation to 1-3 times the mass of the highland barley herb.
3. An alcohol-soluble β -glucan extract, which is produced by the method for producing an alcohol-soluble β -glucan extract according to any one of claims 1 to 2.
4. Use of the alcohol-soluble β -glucan extract according to claim 3 for the preparation of a health wine.
5. A health wine is characterized by comprising white spirit with an alcoholic strength of 40-60% vol and the alcohol-soluble beta-glucan extract as claimed in claim 3, wherein the addition amount of the alcohol-soluble beta-glucan extract is 1-8% of the mass of the white spirit.
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| CN109134695A (en) | 2019-01-04 |
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Address after: 435000 9 Shengming Road, Jinshan street, Huangshi economic and Technological Development Zone, Hubei Province Patentee after: Jingpai Zhengtang Pharmaceutical Co.,Ltd. Address before: No.9, Shengming Road, Jinshan street, Huangshi economic and Technological Development Zone, Huangshi City, Hubei Province Patentee before: JING BRAND BIO-MEDICINE Co.,Ltd. |