CN104886010A - Skin pattern sex-limited trans-breeding method for silkworm variety with common skin pattern - Google Patents
Skin pattern sex-limited trans-breeding method for silkworm variety with common skin pattern Download PDFInfo
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- CN104886010A CN104886010A CN201510236166.8A CN201510236166A CN104886010A CN 104886010 A CN104886010 A CN 104886010A CN 201510236166 A CN201510236166 A CN 201510236166A CN 104886010 A CN104886010 A CN 104886010A
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 32
- 238000009395 breeding Methods 0.000 title abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 37
- 241000382353 Pupa Species 0.000 claims abstract description 10
- 238000009396 hybridization Methods 0.000 claims abstract description 7
- 230000001418 larval effect Effects 0.000 claims abstract description 4
- 230000000384 rearing effect Effects 0.000 claims abstract description 4
- 230000002068 genetic effect Effects 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract 1
- 230000009466 transformation Effects 0.000 description 20
- 239000008280 blood Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 108700005079 Recessive Genes Proteins 0.000 description 3
- 102000052708 Recessive Genes Human genes 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000011426 transformation method Methods 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 230000000306 recurrent effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000009366 sericulture Methods 0.000 description 1
- 210000003765 sex chromosome Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a skin pattern sex-limited trans-breeding method for a silkworm variety with a common skin pattern. The skin pattern sex-limited trans-breeding method comprises the following steps of: carrying out hybridization to obtain F1 by taking a base skin pattern sex-limited variety as a female parent and taking a practical common skin pattern variety as a male parent; back-crossing the F1 and a male individual of the practical common skin pattern variety to obtain BC1, selecting out the common skin pattern individual which is most easily to discriminate by naked eyes at a larval stage from the BC1, selecting an excellent female individual by discriminating pupas, and continuously back-crossing the excellent female individual to BCn; continuously selecting out the individual with a thick common skin pattern from the BCn, and self-crossing and sub-culturing other progeny individuals to Fm-1, self-crossing and carrying out single batch rearing from Fm, selecting an excellent moth area which is in accordance with skin pattern sex-limited characteristics through a pupa discriminating method to perform subculture, and carrying out sex discrimination on every generation by taking the individuals with skin patterns as the female and taking the individuals without skin patterns as the female. According to the method disclosed by the invention, the practicability of a new variety can be improved by continuous back-crossing; the difficulties that the skin pattern depth of a target individual is difficult to discriminate and most of later generations cannot participate in subculture are solved by adopting a method of selecting out the individual with the thickest skin pattern in the later generations; the process is simplified; the operation is simple; the genetic background is wide; and the aim of realizing rapid practical trans-breeding is fulfilled.
Description
Technical field
The invention belongs to silkworm breeding field, be specifically related to the sex-limited transfer method of speckle of a kind of silkworm general spot kind.
Background technology
Speckle sex limited gene by chromosome engineering technology, common speckle (the being called for short general spot) genetic fragment on silkworm autosome is translocated to W sex chromosome (this chromosome has complete decisive action to female in silkworm, everyly have the chromosomal individuality of W just for female) on, make it the sex limited gene with sex determining gene complete linkage.Utilize this speckle sex limited gene general cultivated silkworm breed variety transformation can be become speckle sex limited race, namely every brindled larva is individual is just female, and every larva individuality without spot (plain spot silkworm, white silkworm, Ji silkworm) is just male.It can carry out the sex of identifying by naked eye Different Individual according to the presence or absence of larva speckle, reduce in silkworm silkworm egg reproduction process at different levels and carry out manually in pupa time the conventional processes that " mirror pupa " distinguishes male and female, can reduce the cost of labor more than 20% of silkworm egg reproduction, be currently in Labor-saving breed breeding, apply the most general also one of the most successful technology.But there is a large portion in practicality conventional variety, particularly day Europe system kind, the overwhelming majority is common speckle kind, and current transfer method carries out the sex-limited practical transformation of speckle to this type kind often to be existed and need the problems such as algebraically is many, technology is loaded down with trivial details, the time is long, the degree of being practical of transformation kind is not high.
At present, 3 classes can be summarized as to the method that practicality common speckle kind carries out the sex-limited transformation of speckle:
1, direct transformation method.Choose the sex-limited basic silkworm race of speckle for maternal, hybridize, at its F for male parent to need the practical kind of transformation
2choose that light general spot is female carries out mating subculture, F with plain silkworm hero in generation
3namely the seed selection material in generation has the sex-limited feature of speckle, but still there is following shortcoming in the material obtained: 1. containing needing the practical kind blood relationship composition of transformation to only have 50%, need improve its component ratio further by backcrossing, but the method can not continuous backcross, backcrossing once need every 2 generation; 2. F
2in generation, is chosen in process the offspring individuals only accounting for 1/4 and can meet the method and select requirement to genotype, and other 3/4 individuality can not participate in subculture; 3. at F
2for time accurately select the female individual subculture difficulty of light general spot, the most easily obscure with general spot individuality.(all watersides are precious, Liu Shuqing.The discussion of silkworm egg look and speckle sex limited race selection, " silkworm industry science ", 1986,6 (2): 80-85.)
2, single introductory technique.Choosing the sex-limited basic silkworm race of speckle is female parent, to need the hero of the practical kind of transformation individual for recurrent parent carries out continuous backcross, all the time the female individuality of common speckle and the male individuality of recurrent parent is selected to backcross in each generation backcrossing, until practical kind blood relationship composition can meet the required algebraically of practical requirement, solving like this in direct transformation method can not the difficult problem of continuous backcross, but still there is following shortcoming: must the most easily obscure mutually with light general spot individuality because of speckle depth difference degree reason the female individuality of general spot of M8003 line, and in general spot individuality, also have half to be male individual, M8003 line target individual difficulty.(hold through space, etc., day Europe system shape silkworm transformation becomes the research of sex-limited speckle kind, " Shaanxi silkworm industry ", and 1992,1:5-7; Li Sheng. family's pattern of silkworm sex limited race quick breeding method research. " Agriculture of Anhui science ", 1993,21 (4): 371-373.)
3, independent assortment transformation method.Choose the sex-limited basic silkworm race of speckle for maternal, hybridize, from F for male parent to need the practical kind of transformation
2in generation, starts, the dense general spot individuality that continuous blanking speckle is the darkest, the individuality of other different depth speckle then selects subculture according to the quality of himself actual breeding achievement, this avoid and difficulty (three kinds of brindled larvas are selected to the offspring individuals speckle depth, only have dense general spot individual apparent, general spot individuality and light general spot the individuality then depth are not easily distinguishable), expand the offspring individuals scope that can participate in subculture, but still there is following shortcoming: the blood relationship composition containing the practical kind needing transformation only has 50%, its component ratio need be improved further by the means that backcross, and the method is only isozygotied for the selfing of speckle recessive gene on autosome.(Ai Junwen, etc., the transformation of shape Silkworm varieties becomes the research of speckle sex limited race, " Sericulture of China ", and 2005,1:23-25.)
Summary of the invention
An object of the present invention be for solve in existing method contain selected by need the practical kind blood relationship component ratio of transformation low, the majority individuality of filial generation can not participate in subculture, between offspring individuals, speckle depth difference difficulty is distinguished and affects various difficult problems of required genotypic target individual being carried out to accurately selection, provides the sex-limited transfer method of speckle of a kind of silkworm general spot kind.
The sex-limited transfer method of speckle that the invention provides a kind of silkworm general spot kind comprises hybridization, continuous backcross, selfing, and its step is as follows:
Hybridization: with basic speckle sex limited race for female parent, the general spot kind of practicality is male parent, hybridizes, obtains heterozygous F
1generation;
Continuous backcross: described heterozygous F
1in generation, backcrosses with the male individuality of the general spot kind of practicality and obtains BC
1in generation, from described BC
1play that to eliminate the dense general spot of the easiest naked eyes identification in larval phase individual generation, then choose excellent female individuality by pupa method of reflecting and carry out continuous backcross to BC
ngeneration;
Selfing: from described BC
nwork that to continue to eliminate dense general spot individual generation, other offspring individuals according to they separately actual raise result achievement select and selfing subculture to F
m-1in generation, from described F
min generation, rises and carries out one batch rearing and continue selfing subculture, and choose by mirror pupa method the excellent moth district subculture meeting the sex-limited feature of speckle, each generation is just by there being spot to be female thereafter, is that hero carries out Sex judging without spot.
Beneficial effect of the present invention is: the sex-limited transfer method of speckle of a kind of silkworm of the present invention general spot kind tightly fastens transformation respectively for all not losing speckle sex limited gene (W^ in subculture colony
+) with the inheritance of speckle recessive gene (p), both practicality parent blood relationship component ratio contained by new seed selection material was improved by continuous backcross method, again by the simple and easy way of the denseest individuality of speckle in blanking subculture colony, solve target individual speckle depth difficulty and distinguish the difficulty that can not participate in subculture with most of offspring, the process of achieving is simplified, simple to operate, genetic background is wide, the integration objective that practical transformation is fast.The present invention is compared with silkworm conventional variety selection classical at present, except backcrossing and adding except the measure of the dense general spot individuality of blanking in selfed breeding process, its transformation algebraically, seed selection step, selection are consistent completely, have accomplished that the sex-limited transformation of speckle and the practical seed selection of new material are neglected neither.
Accompanying drawing explanation
Figure 1 shows that the sex-limited transfer method procedure chart of speckle of silkworm of the present invention general spot kind.
Embodiment
Hereafter will describe the specific embodiment of the invention in detail in conjunction with concrete accompanying drawing.It should be noted that the combination of technical characteristic or the technical characteristic described in following embodiment should not be considered to isolated, they can mutually be combined thus be reached better technique effect.
As shown in Figure 1, the sex-limited transfer method of speckle of silkworm of the present invention general spot kind comprises hybridization, continuous backcross, selfing, and its step is as follows:
Hybridization: with basic speckle sex limited race for female parent, the general spot kind of practicality is male parent, hybridizes, obtains heterozygous F
1generation;
Continuous backcross: heterozygous F
1in generation, backcrosses with the male individuality of the general spot kind of practicality and obtains BC
1in generation, from BC
1play that to eliminate the dense general spot of the easiest naked eyes identification in larval phase individual generation, then choose excellent female individuality by pupa method of reflecting and carry out continuous backcross to BC
ngeneration;
Selfing: from BC
nwork that to continue to eliminate dense general spot individual generation, other offspring individuals according to they separately actual breeding achievement select and selfing subculture to F
m-1in generation, from F
min generation, rises and carries out one batch rearing and continue selfing subculture, and choose by mirror pupa method the excellent moth district subculture meeting the sex-limited feature of speckle, each generation is just by there being spot to be female thereafter, is that hero carries out Sex judging without spot.
The sex-limited transfer method of speckle of a kind of silkworm of the present invention general spot kind tightly fastens transformation respectively for all not losing speckle sex limited gene (W^ in subculture colony
+) with the inheritance of speckle recessive gene (p), both practicality parent blood relationship component ratio contained by new seed selection material was improved by continuous backcross method, simple and easy way solution target individual speckle depth difficulty again by the denseest individuality of speckle in blanking subculture colony distinguishes the difficulty that can not participate in subculture with most of offspring, the process of achieving is simplified, simple to operate, genetic background is wide, the integration objective that practical transformation is fast.The present invention is compared with silkworm conventional variety selection classical at present, except backcrossing and adding except the measure of the dense general spot individuality of blanking in selfed breeding process, its transformation algebraically, seed selection step, selection are consistent completely, have accomplished that the sex-limited transformation of speckle and the practical seed selection of new material are neglected neither.
Although give some embodiments of the present invention, it will be understood by those of skill in the art that without departing from the spirit of the invention herein, can change embodiment herein.Above-described embodiment is exemplary, should using embodiment herein as the restriction of interest field of the present invention.
Claims (1)
1. the sex-limited transfer method of speckle of silkworm general spot kind, is characterized in that, comprise hybridization, continuous backcross, selfing, its step is as follows,
Hybridization: with basic speckle sex limited race for female parent, the general spot kind of practicality is male parent, hybridizes, obtains heterozygous F
1generation;
Continuous backcross: described heterozygous F
1in generation, backcrosses with the male individuality of the general spot kind of practicality and obtains BC
1in generation, from described BC
1play that to eliminate the dense general spot of the easiest naked eyes identification in larval phase individual generation, then choose excellent female individuality by pupa method of reflecting and carry out continuous backcross to BC
ngeneration;
Selfing: from described BC
nwork that to continue to eliminate dense general spot individual generation, other offspring individuals according to they separately actual raise result select and selfing subculture to F
m-1in generation, from described F
min generation, rises and carries out one batch rearing and continue selfing subculture, and choose by mirror pupa method the excellent moth district subculture meeting the sex-limited feature of speckle, each generation is just by there being spot to be female thereafter, is that hero carries out Sex judging without spot.
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|---|---|---|---|
| CN201510236166.8A CN104886010B (en) | 2015-05-11 | 2015-05-11 | A kind of sex-limited transfer method of speckle of the general spot kind of silkworm |
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| CN201510236166.8A CN104886010B (en) | 2015-05-11 | 2015-05-11 | A kind of sex-limited transfer method of speckle of the general spot kind of silkworm |
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| CN104886010A true CN104886010A (en) | 2015-09-09 |
| CN104886010B CN104886010B (en) | 2017-07-11 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532588A (en) * | 2015-12-05 | 2016-05-04 | 艾均文 | Simple method using bombyx mori common speckle breed to form practical tea speckle sex-limiting system |
| CN108849773A (en) * | 2018-06-25 | 2018-11-23 | 湖南省蚕桑科学研究所 | A kind of transfer method that the speckle making the Japanese tea spot autosexing race of silkworm is more clear |
| CN110367210A (en) * | 2019-07-26 | 2019-10-25 | 四川省农业科学院蚕业研究所 | The sex-limited speckle dark color transfer method of silkworm |
| CN113080153A (en) * | 2021-05-07 | 2021-07-09 | 云南省农业科学院蚕桑蜜蜂研究所 | Female backcross method for directionally transforming silkworm vegetarian spot variety into tea spot limiting system |
| CN114916508A (en) * | 2022-04-28 | 2022-08-19 | 广西壮族自治区蚕业技术推广站 | Method for effectively fusing and quickly purifying conventional properties and egg color-restricted properties of silkworms |
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| CN104430214A (en) * | 2014-12-31 | 2015-03-25 | 广东省蚕业技术推广中心 | Breeding method and application of robust self-cocoon-emerging stripe double sex-limited marking domestic silkworm variety |
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| 李圣: ""家蚕斑纹限性品种快速选育法研究"", 《安徽农业科学》 * |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532588A (en) * | 2015-12-05 | 2016-05-04 | 艾均文 | Simple method using bombyx mori common speckle breed to form practical tea speckle sex-limiting system |
| CN105532588B (en) * | 2015-12-05 | 2018-03-06 | 湖南省蚕桑科学研究所 | A kind of simple and quick method that the sex-limited system of practicality tea spot is built using the common speckle kind of silkworm |
| CN108849773A (en) * | 2018-06-25 | 2018-11-23 | 湖南省蚕桑科学研究所 | A kind of transfer method that the speckle making the Japanese tea spot autosexing race of silkworm is more clear |
| CN108849773B (en) * | 2018-06-25 | 2021-01-26 | 湖南省蚕桑科学研究所 | Transformation method for enabling stripes of silkworm daily-series tea-dot-restricted variety to be clearer |
| CN110367210A (en) * | 2019-07-26 | 2019-10-25 | 四川省农业科学院蚕业研究所 | The sex-limited speckle dark color transfer method of silkworm |
| CN113080153A (en) * | 2021-05-07 | 2021-07-09 | 云南省农业科学院蚕桑蜜蜂研究所 | Female backcross method for directionally transforming silkworm vegetarian spot variety into tea spot limiting system |
| CN114916508A (en) * | 2022-04-28 | 2022-08-19 | 广西壮族自治区蚕业技术推广站 | Method for effectively fusing and quickly purifying conventional properties and egg color-restricted properties of silkworms |
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Effective date of registration: 20170817 Address after: 410127 Hunan province Changsha Furong District Branch Road No. 23 were Co-patentee after: Soochow University Patentee after: The Sericultural Research Institute of Hunan Province Address before: 410000, No. 8, East Lake Road, Furong district, Hunan, Changsha Co-patentee before: Sima, Yang Hu, Xue Hong Patentee before: Ai Junwen Co-patentee before: Xue Hong |