CN104884168B - 从阱提取受抑制液体 - Google Patents

从阱提取受抑制液体 Download PDF

Info

Publication number
CN104884168B
CN104884168B CN201380058480.0A CN201380058480A CN104884168B CN 104884168 B CN104884168 B CN 104884168B CN 201380058480 A CN201380058480 A CN 201380058480A CN 104884168 B CN104884168 B CN 104884168B
Authority
CN
China
Prior art keywords
chip
fixing device
liquid
component
extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380058480.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN104884168A (zh
Inventor
B·L·格里斯沃尔德
S·A·胡塞恩
R·莫蒂
W·B·唐格
G·康特里
J·邓恩
P·林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bao Biologic Engineering (usa) Co Ltd
Original Assignee
Wafergen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wafergen Inc filed Critical Wafergen Inc
Publication of CN104884168A publication Critical patent/CN104884168A/zh
Application granted granted Critical
Publication of CN104884168B publication Critical patent/CN104884168B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
    • B01L3/50857—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates using arrays or bundles of open capillaries for holding samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F17—STORING OR DISTRIBUTING GASES OR LIQUIDS
    • F17D—PIPE-LINE SYSTEMS; PIPE-LINES
    • F17D1/00—Pipe-line systems
    • F17D1/08—Pipe-line systems for liquids or viscous products
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/126—Paper
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y10T137/0391—Affecting flow by the addition of material or energy
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/8593—Systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Water Supply & Treatment (AREA)
  • Mechanical Engineering (AREA)
  • Public Health (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Extraction Or Liquid Replacement (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
CN201380058480.0A 2012-11-08 2013-11-08 从阱提取受抑制液体 Active CN104884168B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261724118P 2012-11-08 2012-11-08
US61/724,118 2012-11-08
US201361777459P 2013-03-12 2013-03-12
US61/777,459 2013-03-12
PCT/US2013/069214 WO2014074863A2 (en) 2012-11-08 2013-11-08 Extraction of restrained liquid from wells

Publications (2)

Publication Number Publication Date
CN104884168A CN104884168A (zh) 2015-09-02
CN104884168B true CN104884168B (zh) 2017-07-18

Family

ID=50680498

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380058480.0A Active CN104884168B (zh) 2012-11-08 2013-11-08 从阱提取受抑制液体

Country Status (8)

Country Link
US (2) US9447925B2 (enExample)
EP (1) EP2916953B1 (enExample)
JP (1) JP6559568B2 (enExample)
CN (1) CN104884168B (enExample)
AU (1) AU2013342218A1 (enExample)
CA (1) CA2891088C (enExample)
HK (1) HK1214200A1 (enExample)
WO (1) WO2014074863A2 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7622296B2 (en) 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
WO2014074863A2 (en) 2012-11-08 2014-05-15 Wafergen, Inc. Extraction of restrained liquid from wells
WO2016134342A1 (en) 2015-02-20 2016-08-25 Wafergen, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
WO2017087873A1 (en) * 2015-11-18 2017-05-26 Wafergen, Inc. Systems and methods for pooling samples from multi-well devices
CN109070044B (zh) 2016-07-21 2021-07-30 宝生物工程（美国)有限公司 使用多孔装置进行的多z平面成像和分配
CN113832219A (zh) * 2020-06-23 2021-12-24 安徽微分基因科技有限公司 一种基于parms技术的纳升级超高通量snp基因分型方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1946830A1 (en) * 2005-11-07 2008-07-23 Konica Minolta Medical & Graphic, Inc. Microreactor and method of liquid feeding making use of the same
CN101528350A (zh) * 2006-08-30 2009-09-09 因弗因斯医药瑞士股份有限公司 流体指示物装置

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4902481A (en) * 1987-12-11 1990-02-20 Millipore Corporation Multi-well filtration test apparatus
US6083761A (en) 1996-12-02 2000-07-04 Glaxo Wellcome Inc. Method and apparatus for transferring and combining reagents
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
US6274088B1 (en) * 1998-04-06 2001-08-14 Pharmacopeia, Inc. Methods and apparatus for high throughput plate to plate or plate to membrane transfer
DE10026647A1 (de) * 2000-05-29 2001-12-06 Merck Patent Gmbh Positioniervorrichtung
CA2311622A1 (en) * 2000-06-15 2001-12-15 Moussa Hoummady Sub-nanoliter liquid drop dispensing system and method therefor
WO2003028878A1 (en) * 2001-09-28 2003-04-10 Dynametrix Limited Methods and means for creating arrays
US6994544B2 (en) * 2004-01-30 2006-02-07 Texas Instruments Incorporated Wafer scale thermal stress fixture and method
US7622296B2 (en) 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
AU2007217765A1 (en) * 2006-02-21 2007-08-30 Nanogen, Inc. Methods and compositions for analyte detection
AU2008209561A1 (en) 2007-01-22 2008-07-31 Wafergen, Inc. Apparatus for high throughput chemical reactions
CN101896275A (zh) * 2007-12-17 2010-11-24 龚海庆 微流控器件
US20090260458A1 (en) 2008-04-17 2009-10-22 Victor Joseph High throughput dispenser
JP2011062119A (ja) * 2009-09-16 2011-03-31 Seiko Epson Corp 生体試料定量用チップ
WO2012061314A1 (en) * 2010-11-01 2012-05-10 Nanoink, Inc. High-throughput slide processing apparatus
WO2014074863A2 (en) 2012-11-08 2014-05-15 Wafergen, Inc. Extraction of restrained liquid from wells
WO2015116591A1 (en) * 2014-01-30 2015-08-06 Illumina, Inc. Compositions and methods for dispensing reagents

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1946830A1 (en) * 2005-11-07 2008-07-23 Konica Minolta Medical & Graphic, Inc. Microreactor and method of liquid feeding making use of the same
CN101528350A (zh) * 2006-08-30 2009-09-09 因弗因斯医药瑞士股份有限公司 流体指示物装置

Also Published As

Publication number Publication date
EP2916953A4 (en) 2016-06-29
US20170001193A1 (en) 2017-01-05
WO2014074863A2 (en) 2014-05-15
CA2891088A1 (en) 2014-05-15
US9447925B2 (en) 2016-09-20
EP2916953A2 (en) 2015-09-16
US10500585B2 (en) 2019-12-10
AU2013342218A1 (en) 2015-06-04
EP2916953B1 (en) 2023-03-29
US20140130885A1 (en) 2014-05-15
WO2014074863A3 (en) 2014-07-17
HK1214200A1 (zh) 2016-07-22
JP2016508025A (ja) 2016-03-17
JP6559568B2 (ja) 2019-08-14
CA2891088C (en) 2024-01-02
CN104884168A (zh) 2015-09-02

Similar Documents

Publication Publication Date Title
US10500585B2 (en) Extraction of restrained liquid from wells
CN106796166A (zh) 用聚合物捕获膜进行的单细胞捕获
US20120196280A1 (en) Microfabricated device for metering an analyte
JP4665960B2 (ja) 生体試料反応用チップ、生体試料反応装置、および生体試料反応方法
ES2453379T3 (es) Reactivos soportados, métodos y dispositivos
CN102712950B (zh) 用于实施测定法的方法和装置
US8092999B2 (en) Biological sample reaction chip and biological sample reaction method
CN103946392B (zh) 生化分析的系统、设备和方法
CN104508492B (zh) 微流体装置、用于试剂的固体支持体和相关方法
KR20160086937A (ko) 액체 시료들을 탑재하기 위한 시스템 및 방법
EP2227690A1 (en) The micro-chamber plate, manufacturing method thereof
JP2015511016A (ja) 液体試料を充填するためのシステムおよび方法
CN103074203A (zh) 用于核酸扩增反应的微芯片及其制造方法
JP5131538B2 (ja) 反応液充填方法
US20250091043A1 (en) Methods for anchoring lyophilized reagents to a substrate and complexes formed therefrom
US10837048B2 (en) Fluidic card for analysis of biochips
WO2006098435A1 (ja) 検出チップ及びこれを用いた物質の検出方法
JP2009150754A (ja) 生体試料反応用チップ、生体試料反応装置、および生体試料反応方法
HK1200498B (en) Systems, apparatus and methods for biochemical analysis

Legal Events

Date Code Title Description
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1214200

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20180408

Address after: American California

Patentee after: Bao biologic engineering (USA) Co., Ltd.

Address before: American California

Patentee before: Wa Fugen company

REG Reference to a national code

Ref country code: HK

Ref legal event code: WD

Ref document number: 1214200

Country of ref document: HK