CN104877927B - A kind of liquid preparation of utilization livestock breeding wastewater production and its application in continuous cropping obstacle is prevented and kill off - Google Patents
A kind of liquid preparation of utilization livestock breeding wastewater production and its application in continuous cropping obstacle is prevented and kill off Download PDFInfo
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Abstract
The invention discloses a kind of liquid preparation of utilization livestock breeding wastewater production and its application in continuous cropping obstacle is prevented and kill off, it is prepared by bacillus subtilis D9 and the fermented livestock aquaculture wastewater of trichoderma asperellum 12, microorganism total amount ＞ 10 × 10 in the liquid preparation8cfu ml‑1, wherein, effective efficiency microbe colony formation unit >=0.5 × 107cfu ml‑1, bacillus subtilis D9 content ＞ 0.5 × 107cfu ml‑1, trichoderma asperellum T12 content ＞ 0.1 × 106cfu ml‑1Its quantity that can effectively increase cuttage tissue or transplant Biocontrol microorganism around crop seedling root circle, the cuttage tissue or the probability of transplanted seedling of pathogen infection plant in continuous cropping soil are reduced, continuous cropping obstacle is effectively prevented and kill off, while substantial amounts of aquaculture sewage can be consumed.Experiment shows, after the reed wormwood artemisia continuous cropping obstacle soil application product, and the incidence of disease reduces 93.8%, and every mu of reed wormwood artemisia continuous cropping ground 5~8 tons of breeding wastewater that can dissolve；Continuous cropping tomato soil is used after the product, and the incidence of disease reduces 87.3%, every mu of growing tomatoes ground 10~20 tons of aquaculture waste water that can dissolve.
Description
Technical field
The invention belongs to microbial technology field, the liquid preparation produced more particularly to a kind of utilization livestock breeding wastewater
And its application in continuous cropping obstacle is prevented and kill off.
Background technology
With social development, the running of livestock and poultry cultivation multiple enterprises, intensive culture, cultivation density is high, unit aquaculture cost
Reduction.But intensive culture causes livestock and poultry cultivation to produce substantial amounts of discarded object, wherein solid waste can be using at compost
Reason, forms the product of solid, can long range transport to application at it.But a large amount of waste water produced, due to the agriculture on periphery
Field can not dissolve in time, and transport in the form of fertilizer remote farmland, and cost of transportation is too high, breeding enterprise and plantation
Family transport enthusiasm is not high, therefore, and the substantial amounts of irrational disposal of waste water is easy to cause environmental pollution.
Continuous cropping obstacle refers to more than the two batches of the same race or equal crops of continuous plantation in same piece of land, and the plant of continuous cropping occurs
Plant growing way dies down, incidence of disease increase, the phenomenon that product yield and quality decline.The continuous cropping diseases incidence of disease is up to 100%, sternly
The agricultural production of respective crop is threatened again.Soil surface characters quantity increase is the one of the main reasons that soil continuous cropping disease occurs.
Because the capacity of decomposition of soil is very strong, soil adds a small amount of bactericide, it is difficult to soil surface characters be killed, if using high poison agriculture
Medicine-smoking kills germ, it is easy to threaten food security.It is so far to suppress growth of pathogenic bacteria using beneficial biocontrol strains
The method of relatively effective prevention and control continuous cropping obstacle.
Biological organic fertilizer or product with biological pesticide function are applied by specific mode, biocontrol strains are allowed
The guard circle of high concentration can be formed in the surrounding of transplanting or cuttage plant, is conducive to biocontrol strains in the root system of plant
Or colonized on stolon, be conducive to suppressing infecting for pathogen.
Therefore, invent it is a kind of can high additionalization using livestock breeding wastewater energy prevention and control continuous cropping diseases again biological products ten
Divide necessity.So far, do not find to be related to the document report of present subject matter.
The content of the invention
Goal of the invention：In order to overcome the deficiencies in the prior art, the present invention provides one kind and utilizes livestock breeding wastewater
The liquid preparation of production and its application on continuous cropping obstacle is prevented and kill off, the liquid preparation sufficiently utilize livestock breeding wastewater, together
The prevention and control of Shi Jinhang succession crop diseases, effectively prevent and kill off harm of the continuous cropping obstacle to industry.
Technical scheme：In order to solve the above technical problems, the present invention proposes a kind of liquid of utilization livestock breeding wastewater production
Body preparation, described liquid preparation is prepared by bacillus subtilis D9 and the fermented livestock aquaculture wastewater of trichoderma asperellum 12,
Microorganism total amount ＞ 10 × 10 in the liquid preparation8cfu ml-1, wherein, effective efficiency microbe colony formation unit >=0.5 ×
107cfu ml-1, bacillus subtilis D9 content ＞ 0.5 × 107cfu ml-1, trichoderma asperellum T12 content ＞ 0.1 ×
106cfu ml-1。
Wherein, described bacillus subtilis D9 bacterial strain preserving number was CGMCC NO.9170, on May 16th, 2014
China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved in, preservation address is Chaoyang District, Beijing City North Star west
The institute 3 of road 1；The bacterial strain preserving number of described trichoderma asperellum 12 is CCTCC NO：M2013213, was protected on May 16th, 2013
China typical culture collection center is hidden in, preservation address is Wuhan, China Wuhan University.
Present invention further proposes a kind of above-mentioned preparation method of the liquid preparation of utilization livestock breeding wastewater production, bag
Include following steps：
(1) bacillus subtilis D9 is inoculated into bacillus subtilis liquid fermentation medium, bacillus subtilis is made
Bacterium D9 zymotic fluids；Wherein, the condition of fermenting and producing is：Initial pH scopes are 6.5~7.2, and 35~37 DEG C of cultivation temperature, dissolved oxygen leads to
Tolerance scope is 30~100%, 120~180rpm, and ferment 16~18h, obtains bacillus subtilis D9 zymotic fluids；
(2) trichoderma asperellum 12 is inoculated into PDA nutrient solutions, carries out liquid fermentation production, obtain the zymotic fluid of trichoderma asperellum 12,
The condition of its fermenting and producing is：Initial pH scopes are 6.5~7.2,25~30 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30~
100%, 150~170rpm, ferment 30~36h, and fermentation later stage mycelium pellet is all broken into mycelia fragment, the bacterium of zymotic fluid bacterial strain
Fall to form unit >=1 × 108cfu ml-1；
(3) zymotic fluid of trichoderma asperellum 12 that step (2) is obtained is inoculated into solid fermentation matrix, inoculum concentration is volume ratio
5%, 23~28 DEG C, ferment 5~8 days, low temperature drying crushed 200 mesh sieves, obtained the solid bacterium powder of trichoderma asperellum 12；
(4) livestock breeding wastewater is decomposed with microbial bacterial agent, flocculation sediment, remove breeding wastewater in it is substantial amounts of solid
Shape particulate matter, roundworm egg, the microorganism of part, obtain livestock and poultry cultivation sewage；
(5) bacillus subtilis D9 liquid fermentation liquids and trichoderma asperellum T12 solid bacterium powders are added to the cultivation after processing
In waste water, bacillus subtilis D9 liquid spawns are according to 2~10L/m3Livestock and poultry cultivation sewage, trichoderma asperellum T12 solid bacterium powders are pressed
According to 2~10kg/m3The ratio of livestock and poultry cultivation sewage is respectively added in the livestock and poultry cultivation sewage that step (4) is obtained, and will add bacterium
The livestock and poultry cultivation sewage planted is transferred to aerated fermentation groove, and bottom land supply is supplied by warmhouse booth or solar converter in bottom land
Heat, it is ensured that the sewage temperature of fermentation is no more than 30 DEG C；Fermentation 5~8 days, produces a kind of preventing and kill off for utilization livestock breeding wastewater production
The product of continuous cropping obstacle.
Specifically, in step (1), liquid fermentation medium used is measured with volume 1L, includes beef extract 3g, peptone
10g, NaCl 3g, running water 1000ml, pH scope 7.0~7.5, in standby after 121 DEG C of 20min that sterilize.
In step (2), PDA culture liquid making methods used are：Exemplified by preparing 1L culture mediums, peeled with 100g potatoes
After be cut into small pieces to be put into water and boil, 30min is boiled after boiling, after filtering in filtrate plus 5g common sucroses and 2g rice straw powders,
1000ml, pH value are settled to naturally, in standby after 115 DEG C of 30min that sterilize.
In step (3), the preparation method of solid fermentation matrix used is：Maize cob meal：Wheat bran is 1 according to mass ratio
: 1, water content 50~55% is adjusted, in standby after 120 DEG C of 30min that sterilize.
Continuous cropping barrier is being prevented and kill off present invention further proposes the liquid preparation of above-mentioned utilization livestock breeding wastewater production
Application in hindering.
Specifically, in the application：
The crop bred for cuttage seeding, stirs continuous cropping soil, soil, which is stirred, to be finished, and is adopted using the rotary cultivator with pressure roller
After conventional drilling method punching, the product for preventing and kill off continuous cropping obstacle for being produced a kind of utilization livestock breeding wastewater using small pump
Uniformly spray in the surface of field face soil；Poured using the water pipe with shower nozzle, this product in field face is poured into above-mentioned beat
Hole in, field face is stablized 1~2 day, cuttage stalk；
For the crop using transplanted seedling, soil stirs crushing, stirs soil, a ditch is opened in field face, into trench digging
Pour and apply this product, soil stabilization transplants crop in the ditch that field face is opened after 1~2 day.
Beneficial effect：Compared with prior art, a kind of company of preventing and kill off for utilization livestock breeding wastewater production that the present invention is provided
The product for making obstacle is the microorganism one by being obtained with the microbial strains fermented livestock breeding wastewater that biological control is acted on
The product for preventing and kill off continuous cropping obstacle produced using livestock breeding wastewater is planted, is had compared with product in the market following excellent
Point：
(1) a kind of product for preventing and kill off continuous cropping obstacle of utilization livestock breeding wastewater of the invention production being capable of dividing by water
Dissipate to act on and be distributed highdensity biocontrol microorganisms around transplanted seedling or cuttage seeding in the range of 10~20cm, can be effectively protected
The root system of plant is from invading pathogens in continuous cropping soil；
(2) every mu of soil of part industrial crops can apply 5~20 tons of product of the present invention, be hundred pig cultivation bases 2~
The wastewater displacement of 10 days, the sewage that the aquaculture that substantial amounts of can dissolve is produced；
(3) waste water is after high added valueization processing, and the planting households of enterprise periphery industrial crops has higher positive
Property use the product, can effectively reduce the cost that breeding enterprise handles waste water, cause environment dirty while reducing discharge of wastewater
Dye.
Brief description of the drawings
The biological and ecological methods to prevent plant disease, pests, and erosion efficiency of Fig. 1 different disposal continuous cropping reed wormwood artemisia diseases；
Soil microbe quantity at Fig. 2 different disposal tomato continuous croppings 20cm；
The incidence of disease of Fig. 3 different disposal continuous cropping soil tomatoes.
Embodiment
The present invention is further described with reference to embodiment.
Wherein：
Trichoderma asperellum 12 (Trichoderma asperellum 12), bacterial strain preserving number is CCTCC NO：M2013213,
China typical culture collection center is preserved on May 16th, 2013, preservation address is Wuhan, China Wuhan University.
Bacillus subtilis D9 (Bacillus subtilis D9) bacterial strain preserving number is CGMCC NO.9170, in 2014
It is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 16, in, preservation address is court of Beijing
The positive institute 3 of area's North Star West Road 1.
Embodiment 1 is used for the preparation for preventing and kill off the liquid preparation of continuous cropping obstacles.
(1) microbial inoculum is produced
(1) bacillus subtilis D9 is inoculated into bacillus subtilis liquid fermentation medium, bacillus subtilis is made
Zymotic fluid.The condition of its fermenting and producing is：Initial pH scopes are 6.5~7.2,35~37 DEG C of cultivation temperature, dissolved oxygen throughput model
Enclose for 30~100%, 120~180rpm, fermentation 18h, acquisition bacillus subtilis D9 zymotic fluids；Wherein, liquid used hair
Ferment culture medium with volume 1L measure, comprising beef extract 3g, peptone 10g, NaCl 3g, running water 1000ml, pH scope 7.0~
Used after 7.5,121 DEG C of sterilizing 20min.
(2) trichoderma asperellum 12 is inoculated into PDA nutrient solutions, carries out liquid fermentation production, obtain the zymotic fluid of trichoderma asperellum 12,
The condition of its fermenting and producing is：Initial pH scopes are 6.5~7.2,25~30 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30~
100%, 170rpm, ferment 30h, and fermentation later stage mycelium pellet is all broken into mycelia fragment, and the bacterium colony of zymotic fluid bacterial strain forms list
Position >=1 × 108cfu ml-1；PDA cultures liquid making method used is (exemplified by preparing 1L culture mediums)：Peeled with 100g potatoes
After be cut into small pieces to be put into water and boil, 30min is boiled after boiling, after filtering in filtrate plus 5g common sucroses and 2g rice straw powders,
1000ml, pH value are settled to naturally, 115 DEG C of sterilizing 30min.
(3) zymotic fluid of trichoderma asperellum 12 is inoculated into solid fermentation matrix, inoculum concentration be volume ratio 5%, 23~28 DEG C,
Fermentation 5 days, low temperature drying crushed 200 mesh sieves, obtained the solid bacterium powder of trichoderma asperellum 12；Solid fermentation matrix used is by jade
Rice core powder and wheat bran are 1: 1 according to mass ratio, and regulation water content to 50~55% is prepared, and in 120 DEG C of sterilizing 30min
It is standby afterwards.
(2) livestock and poultry cultivation sewage ferments
(1) by livestock breeding wastewater, with the microbial bacterial agent purchased from Environmental Research Institute of Hohai University, (main bacteria seed is Xie Dian
Afnyloliquefaciens, bacillus thuringiensis and trichoderma asperellum) decomposed, flocculation sediment, it is substantial amounts of in breeding wastewater to remove
Solid particles thing, roundworm egg, the microorganism of part, obtain livestock and poultry cultivation sewage；
(2) bacillus subtilis D9 liquid fermentation liquids and trichoderma asperellum T12 solid bacterium powders are added to the livestock and poultry after processing
In breeding wastewater (i.e. livestock and poultry cultivation sewage), wherein, bacillus subtilis D9 liquid bacterias addition is 2L/m in aquaculture wastewater3,
Trichoderma asperellum T12 solid bacterium powders addition is 2kg/m3.Fermentation tank is built in warmhouse booth, groove width 1.2m, groove depth 2m will be added
The aquaculture wastewater of strain is transferred to aerated fermentation groove, and bottom land is supplied with diameter 20mm pvc pipe, with 5 layings of diameter 30mm iron pipes
In fermentation trench bottom, water is heated by solar energy heat absorption pipe, heat energy is led and heats aquaculture wastewater in trench bottom, it is ensured that fermentation sewage
Temperature be no more than 30 DEG C；Fermentation 8 days, that is, obtain a kind of product for preventing and kill off continuous cropping obstacle of utilization livestock breeding wastewater production,
Wherein, microorganism total amount ＞ 10 × 10 in product8cfu ml-1, bacillus subtilis D9 content ＞ 0.5 × 107cfu ml-1,
Trichoderma asperellum T12 content ＞ 0.1 × 106cfu ml-1。
Embodiment 2 is used for the preparation for preventing and kill off the liquid preparation of continuous cropping obstacles.
(1) microbial inoculum is produced
(1) bacillus subtilis D9 is inoculated into bacillus subtilis liquid fermentation medium, bacillus subtilis is made
Zymotic fluid.The condition of its fermenting and producing is：Initial pH scopes are 6.5~7.2,35~37 DEG C of cultivation temperature, dissolved oxygen throughput model
Enclose for 30~100%, 120~180rpm, fermentation 16h, acquisition bacillus subtilis D9 zymotic fluids；Wherein, liquid used hair
Ferment culture medium with volume 1L measure, comprising beef extract 3g, peptone 10g, NaCl 3g, running water 1000ml, pH scope 7.0~
Used after 7.5,121 DEG C of sterilizing 20min.
(2) trichoderma asperellum 12 is inoculated into PDA nutrient solutions, carries out liquid fermentation production, obtain the zymotic fluid of trichoderma asperellum 12,
The condition of its fermenting and producing is：Initial pH scopes are 6.5~7.2,25~30 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30~
100%, 170rpm, ferment 36h, and fermentation later stage mycelium pellet is all broken into mycelia fragment, and the bacterium colony of zymotic fluid bacterial strain forms list
Position >=1 × 108cfu ml-1；PDA cultures liquid making method used is (exemplified by preparing 1L culture mediums)：Peeled with 100g potatoes
After be cut into small pieces to be put into water and boil, 30min is boiled after boiling, after filtering in filtrate plus 5g common sucroses and 2g rice straw powders,
1000ml, pH value are settled to naturally, 115 DEG C of sterilizing 30min.
(3) zymotic fluid of trichoderma asperellum 12 is inoculated into solid fermentation matrix, inoculum concentration be volume ratio 5%, 23~28 DEG C,
Fermentation 8 days, low temperature drying crushed 200 mesh sieves, obtained the solid bacterium powder of trichoderma asperellum 12；Solid fermentation matrix used is by jade
Rice core powder and wheat bran are 1: 1 according to mass ratio, and regulation water content to 50~55% is prepared, and in 120 DEG C of sterilizing 30min
It is standby afterwards.
(2) livestock and poultry cultivation sewage ferments
(1) by livestock breeding wastewater, with the microbial bacterial agent purchased from Environmental Research Institute of Hohai University, (main bacteria seed is Xie Dian
Afnyloliquefaciens, bacillus thuringiensis and trichoderma asperellum) decomposed, flocculation sediment, it is substantial amounts of in breeding wastewater to remove
Solid particles thing, roundworm egg, the microorganism of part, obtain livestock and poultry cultivation sewage；
(2) bacillus subtilis D9 liquid fermentation liquids and trichoderma asperellum T12 solid bacterium powders are added to the livestock and poultry after processing
In breeding wastewater (i.e. livestock and poultry cultivation sewage), wherein, bacillus subtilis D9 liquid bacterias addition is 10L/m in aquaculture wastewater3,
Trichoderma asperellum T12 solid bacterium powders addition is 10kg/m3.Fermentation tank is built in warmhouse booth, groove width 1.2m, groove depth 2m will add
Plus the aquaculture wastewater of strain is transferred to aerated fermentation groove, bottom land is supplied with diameter 20mm pvc pipe, with 5 pavings of diameter 30mm iron pipes
Located at fermentation trench bottom, water is heated by solar energy heat absorption pipe, heat energy is led and heats aquaculture wastewater in trench bottom, it is ensured that fermentation is dirty
The temperature of water is no more than 30 DEG C；Fermentation 8 days, that is, obtain a kind of production for preventing and kill off continuous cropping obstacle of utilization livestock breeding wastewater production
Product, wherein, microorganism total amount ＞ 10 × 10 in product8cfu ml-1, bacillus subtilis D9 content ＞ 0.5 × 107cfu ml-1, trichoderma asperellum T12 content ＞ 0.1 × 106cfu ml-1。
Application of the liquid preparation in reed wormwood artemisia continuous cropping obstacle ground for being used to prevent and kill off continuous cropping obstacle of the present invention of embodiment 3.
Experimental field addressing Nanjing Qixia District, soil continuously plants reed wormwood artemisia 5 years, the 4th year Winter-Spring reed wormwood artemisia incidence of disease 9%,
The 5th year Winter-Spring reed wormwood artemisia incidence of disease 100%.
Experiment sets 3 processing, is respectively：
(1) control treatment：General planting；
(2) cultivating wastewater purification：Continuous cropping soil applies ton/mu of breeding wastewater 5；
(3) product treatment of the present invention：Continuous cropping soil apply the present invention product (embodiment 1 prepare prevent and kill off continuous cropping obstacle
Liquid preparation) 5 tons/mu；
It is great Ye wormwood artemisias to plant reed wormwood artemisia kind, stirs continuous cropping soil using the rotary cultivator with pressure roller, stirs depth 15cm, soil
Earth Manual punching sprays breeding wastewater or product of the present invention after finishing, spray after finishing, and every mu of soil waters 5m3, by native table
Waste water or product of the present invention are rinsed into the hole manually beaten, soil stabilization 48h, seedlings plugging.
After seedlings plugging 50 days, the incidence of disease of the accumulation of 3 processing is determined, as a result as shown in figure 1, the incidence of disease of control treatment
100%, wastewater treatment and product treatment of the present invention can prevention and control continuous cropping diseases, wherein wastewater treatment well diseases prevention rate
30.6%, the diseases prevention rate 93.8% of product of the present invention, product of the present invention can be good at preventing and kill off the generation of continuous cropping obstacle, and every mu
Can dissolve to reed wormwood artemisia continuous cropping 5~8 tons of breeding wastewater.
Application of the liquid preparation on tomato continuous cropping obstacle ground for being used to prevent and kill off continuous cropping obstacle of the present invention of embodiment 4.
Experimental field addressing Jiangsu Suqian Xiuyu District, plants tomato green plastic shed soil, upper season tomato bacterial wilt hair
Sick rate 100%, bacterial wilt is bacterial disease, is caused by Ralstonia solanacearum.
Experiment sets 3 processing, is respectively：
(1) control treatment：General planting method；
(2) cultivating wastewater purification, ton/mu of soil application waste water 15；
(3) product treatment of the present invention, soil application product of the present invention (liquid preparation prepared by embodiment 2) 15 tons/mu.
Tomato booth soil is stirred using rotary cultivator, and soil stirs depth 25cm, and soil is organized into furrow, by breeding wastewater
Or product of the present invention is poured in that will transplant the soil of tomato seedling, is poured several times, it is ensured that by breeding wastewater or this hair
Bright product is poured into soil, after soil pouring is finished, stablizes 5~10 days, transplants tomato seedling.
Edaphon is determined before transplanting and shown, compared to control treatment, wastewater treatment and product treatment of the present invention away from
From bacillus total amount in soil at earth's surface 20cm significantly rise (as shown in Figure 2), but wastewater treatment Trichoderma quantity with
Control is not significantly different.Compared to control treatment, soil Trichoderma quantity adds 33 times, gemma at present invention processing 20cm
Bacillus quantity adds 181 times, therefore product of the present invention can significantly increase the beneficial microbe number in transplanted seedling root ring border
Amount.
After tomato is planted 60 days, the incidence of disease of the accumulation of measure is as shown in figure 3, the control treatment incidence of disease 100%, western red
Persimmon mainly receives cumulative morbidity 56.2% He of difference of the harm of bacterial wilt, wastewater treatment and product treatment disease of the present invention
12.7%.The generation of product of the present invention with can significantly suppressing tomato continuous cropping obstacle disease, reaches to the control rate of disease
87.3%, while every mu of growing tomatoes ground 10~20 tons of aquaculture waste water that can dissolve.
Described above is only the preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (7)
1. a kind of liquid preparation of utilization livestock breeding wastewater production, it is characterised in that the liquid preparation passes through withered grass gemma
Bacillus (Bacillus subtilis) D9 and the fermented livestock aquaculture wastewater of trichoderma asperellum (Trichoderma asperellum) 12
Prepare, microorganism total amount ＞ 10 in the liquid preparation for preventing and kill off continuous cropping obstacle of utilization livestock breeding wastewater production ×
108cfu ml-1, wherein, effective efficiency microbe colony formation unit >=0.5 × 107cfu ml-1, bacillus subtilis
(Bacillussubtilis) D9 content ＞ 0.5 × 107cfu ml-1, trichoderma asperellum (Trichoderma asperellum)
T12 content ＞ 0.1 × 106cfu ml-1, wherein, described bacillus subtilis (Bacillussubtilis) D9 bacterial strain
Preserving number is CGMCC NO.9170, is preserved in China Committee for Culture Collection of Microorganisms on May 16th, 2014 commonly micro-
Bio-Centers, preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Described trichoderma asperellum (Trichoderma
Asperellum) 12 bacterial strain preserving number is CCTCC NO：M2013213, Chinese Typical Representative training is preserved on May 16th, 2013
Thing collection is supported, preservation address is Wuhan, China Wuhan University.
2. the preparation method of the liquid preparation of the utilization livestock breeding wastewater production described in claim 1, it is characterised in that including
Following steps：
(1) bacillus subtilis (Bacillus subtilis) D9 is inoculated into bacillus subtilis liquid fermentation medium,
Bacillus subtilis (Bacillus subtilis) D9 zymotic fluids are made；Wherein, the condition of fermenting and producing is：Initial pH scopes
For 6.5~7.2,35~37 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30~100%, 120~180rpm, fermentation 16~
18h, obtains bacillus subtilis (Bacillussubtilis) D9 zymotic fluids；
(2) trichoderma asperellum (Trichoderma asperellum) 12 is inoculated into PDA nutrient solutions, carries out liquid fermentation production,
Trichoderma asperellum (Trichoderma asperellum) 12 zymotic fluids are obtained, the condition of its fermenting and producing is：Initially pH scopes are
6.5~7.2,25~30 DEG C of cultivation temperature, dissolved oxygen throughput scope is 30~100%, 150~170rpm, and ferment 30~36h,
Fermentation later stage mycelium pellet is all broken into mycelia fragment, CFU >=1 × 10 of zymotic fluid bacterial strain8cfu ml-1；
(3) trichoderma asperellum (Trichoderma asperellum) 12 zymotic fluids obtained step (2) are inoculated into solid fermentation
In matrix, inoculum concentration is volume ratio 5%, 23~28 DEG C, is fermented 5~8 days, and low temperature drying crushed 200 mesh sieves, obtained spine spore
The solid bacterium powder of trichoderma (Trichoderma asperellum) 12；
(4) livestock breeding wastewater is decomposed with microbial bacterial agent, flocculation sediment, remove breeding wastewater in substantial amounts of solid
Grain thing, roundworm egg, the microorganism of part, obtain livestock and poultry cultivation sewage；
(5) by bacillus subtilis (Bacillus subtilis) D9 liquid fermentation liquids and trichoderma asperellum (Trichoderma
Asperellum) 12 solid bacterium powders are added in the breeding wastewater after processing, bacillus subtilis (Bacillus subtilis)
D9 liquid spawns are according to 2~10L/m3Livestock and poultry cultivation sewage, trichoderma asperellum (Trichoderma asperellum) 12 solid bacteriums
Powder is according to 2~10kg/m3The ratio of livestock and poultry cultivation sewage is respectively added in the livestock and poultry cultivation sewage that step (4) is obtained, and will be added
Plus the livestock and poultry cultivation sewage of strain is transferred to aerated fermentation groove, bottom land supply, by warmhouse booth or solar converter in groove
Bottom heat supply, it is ensured that the sewage temperature of fermentation is no more than 30 DEG C；Fermentation 5~8 days, produces one kind and produces liquid using livestock breeding wastewater
Body preparation.
3. preparation method according to claim 2, it is characterised in that in step (1), liquid fermentation medium used with
Volume 1L is measured, comprising beef extract 3g, peptone 10g, NaCl 3g, running water 1000ml, pH scope 7.0~7.5, in 121 DEG C
It is standby after sterilizing 20min.
4. preparation method according to claim 2, it is characterised in that in step (2), PDA nutrient solutions preparation side used
Method is：Exemplified by preparing 1L culture mediums, it is cut into small pieces to be put into water after being peeled with 100g potatoes and boils, 30min is boiled after boiling, passes through
Add 5g common sucroses and 2g rice straw powders after filter in filtrate, be settled to 1000ml, pH value naturally, in after 115 DEG C of 30min that sterilize
It is standby.
5. preparation method according to claim 2, it is characterised in that in step (3), the system of solid fermentation matrix used
Preparation Method is：Maize cob meal：Wheat bran is 1: 1 according to mass ratio, adjusts water content 50~55%, in after 120 DEG C of 30min that sterilize
It is standby.
6. liquid preparation the answering in continuous cropping obstacles are prevented and kill off of the utilization livestock breeding wastewater production described in claim 1
With.
7. application according to claim 6, its spy is, in the application：
The crop bred for cuttage seeding, stirs continuous cropping soil, soil, which is stirred, to be finished, using normal using the rotary cultivator with pressure roller
After the drilling method punching of rule, the liquid preparation for preventing and kill off continuous cropping obstacle for being produced the utilization livestock breeding wastewater using small pump
Uniformly spray in the surface of field face soil；Poured using the water pipe with shower nozzle, the utilization livestock and poultry cultivation in field face is given up
The liquid preparation for preventing and kill off continuous cropping obstacle of aquatic production is poured in above-mentioned beaten hole, and field face is stablized 1~2 day, cuttage stalk；
For the crop using transplanted seedling, soil stirs crushing, stirs soil, a ditch is opened in field face, pours and applies into trench digging
The liquid preparation for preventing and kill off continuous cropping obstacle of utilization livestock breeding wastewater production, soil stabilization is after 1~2 day, transplant crop in
In the ditch that field face is opened.
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| CN104877927B true CN104877927B (en) | 2017-11-03 |
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| CN201510119919.7A Active CN104877927B (en) | 2015-03-18 | 2015-03-18 | A kind of liquid preparation of utilization livestock breeding wastewater production and its application in continuous cropping obstacle is prevented and kill off |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102642979A (en) * | 2012-01-20 | 2012-08-22 | 湖南海尚环境生物科技有限公司 | Livestock and poultry breeding wastewater treatment method |
| CN103396954A (en) * | 2013-07-22 | 2013-11-20 | 河海大学 | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer |
| CN103449672A (en) * | 2013-09-05 | 2013-12-18 | 湖北特微生物环保有限公司 | Treatment method and recycling method of livestock and poultry breeding wastewater and biogas slurry |
| CN104016745A (en) * | 2014-06-12 | 2014-09-03 | 河海大学 | Microorganism organic fertilizer with functions of preventing and eliminating successive cropping obstacle of artemisia selengensis and preparation method thereof |
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| CN102642979A (en) * | 2012-01-20 | 2012-08-22 | 湖南海尚环境生物科技有限公司 | Livestock and poultry breeding wastewater treatment method |
| CN103396954A (en) * | 2013-07-22 | 2013-11-20 | 河海大学 | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer |
| CN103449672A (en) * | 2013-09-05 | 2013-12-18 | 湖北特微生物环保有限公司 | Treatment method and recycling method of livestock and poultry breeding wastewater and biogas slurry |
| CN104016745A (en) * | 2014-06-12 | 2014-09-03 | 河海大学 | Microorganism organic fertilizer with functions of preventing and eliminating successive cropping obstacle of artemisia selengensis and preparation method thereof |
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