CN104877906B - Atomizing plant cultivation reactor - Google Patents
Atomizing plant cultivation reactor Download PDFInfo
- Publication number
- CN104877906B CN104877906B CN201510249731.4A CN201510249731A CN104877906B CN 104877906 B CN104877906 B CN 104877906B CN 201510249731 A CN201510249731 A CN 201510249731A CN 104877906 B CN104877906 B CN 104877906B
- Authority
- CN
- China
- Prior art keywords
- negative pressure
- spraying
- retort
- generation tank
- pressure bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The present invention provides a kind of atomizing plant cultivation reactors, which is characterized in that including：Retort and the spray assembly being arranged below the retort, the spray assembly includes the spraying generation tank and negative pressure bottle that set gradually from top to bottom, by being connected downspout, communicating pipe between the retort and negative pressure bottle, the bottom of the spraying generation tank is equipped with stalk, the stalk is deeply to the bottom of negative pressure bottle, the top of the spraying generation tank is additionally provided with injection spray pipe and communicating pipe, the communicating pipe is connected with the top of negative pressure bottle, the injection spray pipe extends to the top in fixed seedbed, and the end of the injection spray pipe is equipped with fog spray head.Advantageous effect is possessed by the present invention：The present invention has reached spraying and has formed required pressure, the effect of spraying is preferable, ingenious in design, of low cost, has the prospect of industrial application by setting downspout and communicating pipe.
Description
Technical field
The present invention relates to a kind of device of biotechnology, specifically a kind of plant spray formula cultivation reactor.
Background technology
The exploitation of phytochemicals production always is the hot spot of research, and Vitro Plant tissue is carried out using bioreactor
Culture can not only produce many secondary metabolites with important value, but also can alleviate excessively adopting for wild resource
It digs.Reactor currently used for medicinal plant tissue, cell and Organ culture mainly has stirring-type, gas-lifting type and atomizing
Deng.Stirring type bioreactor opereating specification is big, and oxygen delivery capacity is strong, good mixing effect, but plant tissue in whipping process to producing
Raw shearing force is especially sensitive.Although airlift reactor shearing force is small, the mixing and gas that are unfavorable for substance are transmitted.Spraying
Formula reactor can by moisture content in the form of fog sustainable supply plant tissue, farthest reduce the shearing force of liquid,
In turn avoiding traditional reactor simultaneously makes plant tissue be immersed in liquid for a long time and lead to anoxic, therefore be current Vitro Plant group
Knit used ideal reactor type during culture.
To solve the above-mentioned problems, Chinese patent CN103031251A and Chinese patent CN101457205 are individually disclosed
A kind of multi-functional plant excised root culture bioreactor system and a kind of siphoning type intermittent spraying bioreactor, but it is above-mentioned
Two kinds of reactors are due to being not achieved the pressure needed for spraying, so " droplet " that is formed is larger, the effect of spraying is undesirable.
Invention content
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of plant spray formula cultivation reactors.
The present invention is achieved by the following technical solutions：
The present invention provides a kind of atomizing plant cultivation reactor, including：The retort that sets gradually from top to bottom,
Spraying generation tank and negative pressure bottle, the bottom of the retort are connected with the top of negative pressure bottle by downspout, the spraying hair
Raw tank is connected with negative pressure bottle by stalk and communicating pipe, and the stalk is drawn by the bottom of spraying generation tank, is extended to
The bottom of negative pressure bottle, the communicating pipe by negative pressure bottle Base top contact, extend to spraying generation tank top, it is described spraying occur
The side wall of tank is also communicated with the injection spray pipe extended in retort.
Preferably, the top of the retort is equipped with air filter.
Preferably, the middle part of the stalk is equipped with liquid pump.
Preferably, the retort and the distance of spraying generating bottle are not less than 30cm, no more than 40cm.
Preferably, the distance between liquid level and injection spray pipe top are not less than 12cm in the spraying generation tank, no
More than in 15cm.
Liquid in retort is transferred to by downspout in negative pressure bottle, and then the liquid in negative pressure bottle acts in liquid pump
Under via stalk be transferred to spraying generating bottle in, the pressure in negative pressure bottle leads to mist by being transferred to mist generating bottle communicating pipe
The raising of pressure in generating bottle, and released pressure in the form of spraying.There is provided a root long degree in the present invention to be more than
The downspout of 30cm, pressure caused by pressure and the liquid pump operating that liquid generates in the downspout, it is whole to pass through communicating pipe
It is transferred in spraying generation tank, so as to dramatically increase the pressure in spraying generation tank, and ultimately forms " ideal " spraying effect
Fruit.
A kind of application method of atomizing plant cultivation reactor using the present invention, includes the following steps：In asepsis ring
Under border, fresh fluid nutrient medium is injected into retort first, while open liquid pump, control pump speed is 0.5~1ml/s；
When the liquid level in negative pressure bottle and spraying generating bottle is 2/3rds or so of bottle height, fresh medium implant operation stops
Only；Then germ-free plant material is transferred in culture tank, and is 0.05~0.1ml/s by pump speed fixed setting.
Compared with prior art, the present invention has following advantageous effect：
1st, it is simple in structure, it is easy to assembly；
2nd, the defects of droplet is larger is overcome, the liquid in retort is made to pass through downspout and is transferred in negative pressure bottle, then
Liquid in negative pressure bottle is transferred to via stalk in mist generating bottle under being acted in liquid pump, and the pressure in negative pressure bottle passes through connection
Pipe is transferred to spraying generating bottle, leads to the raising of pressure in mist generating bottle, and is released pressure in the form of spraying.
Description of the drawings
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon：
Fig. 1 is the structural diagram of the present invention.
In figure：1st, air filter, 2, retort, 3, spraying generation tank, 4, downspout, 5, fixed seedbed, 6, fog spray head, 7,
Injection spray pipe, 8, liquid pump, 9, stalk, 10, negative pressure bottle, 11, communicating pipe.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill to this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection domain.
Embodiment 1
The present embodiment be related to a kind of structure of atomizing plant cultivation reactor as shown in Figure 1, including：From top to bottom successively
Retort 2, spraying generation tank 3 and the negative pressure bottle 10 in the built-in fixed seedbed 5 of setting, bottom and the negative pressure bottle 10 of retort 2
Top is connected by downspout 4, and spraying generation tank 3 is connected with negative pressure bottle 10 by stalk 9 and communicating pipe 11, stalk
9 are drawn by the bottom of spraying generation tank 3, are extended to the bottom of negative pressure bottle 10, by the Base top contact of negative pressure bottle 10, are prolonged communicating pipe 11
The top of spraying generation tank 3 is extended to, the side wall of spraying generation tank 3 is also communicated with the injection spray pipe 7 extended in retort 2, sprays
The outlet of pipe 7 is equipped with fog spray head 6, and the atomizing nozzle face of fog spray head 6 fixes seedbed 5.
In order to make that germ-free condition is kept in retort, air filter 1 is equipped at 2 top of retort.
For the water in negative pressure bottle 10 is enable smoothly to be transferred in spraying generation tank 3, liquid is provided at the middle part of stalk 9
Body pump 8.
For the pressure for making the liquid generation in downspout enough, the distance of the retort and negative pressure bottle is more than 30cm,
No more than 40cm.
It is described in order to farthest reduce counteracting of the pressure of liquid generation in injection spray pipe to generation pressure in downspout
The distance between liquid level and injection spray pipe top are less than 15cm in spraying generation tank.
The present invention operation principle be：Liquid in retort is transferred to by downspout in negative pressure bottle, then negative pressure bottle
In liquid acted in liquid pump under be transferred in spraying generation tank via stalk, pressure in negative pressure bottle, which passes through, to be turned communicating pipe
Spraying generation tank is moved on to, leads to the raising of pressure in spraying generation tank, and is released pressure in the form of spraying.The present invention
In be provided with the downspout that a root long degree is not less than 30cm, produced by pressure and the liquid pump operating that liquid generates in the downspout
Pressure, by be transferred completely into communicating pipe spraying generation tank in, so as to dramatically increase the pressure in spraying generation tank, and most
End form is into " ideal " spray effect.
In use, in an aseptic environment, fresh fluid nutrient medium is injected into retort first, while open liquid
Pump, control pump speed are 0.5~1ml/s；When the liquid level in negative pressure bottle and spraying generating bottle is 2/3rds of bottle height, newly
Fresh culture solution implant operation stops；Then germ-free plant material is transferred in culture tank, and is 0.05 by pump speed fixed setting
~0.1ml/s.
Implementation result
The volume of retort is 1 liter in the reactor of the present embodiment, and the length of downspout 4 is 40cm, in generation tank of spraying
The distance between liquid level and injection spray pipe top are 10cm.For the present embodiment to periwinkle Aseptic seedling culture, culture solution is addition
The MS culture mediums of NAA and 6-BA, the rotating speed of liquid pump is 0.1ml/s, and cultivation temperature is 25 DEG C.The result shows that being suitable for plant
Under conditions of cell growth (25 DEG C), periwinkle all took root, and grow fine at 30 days.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring the substantive content of the present invention.
Claims (2)
1. a kind of atomizing plant cultivation reactor, which is characterized in that including：The retort that sets gradually from top to bottom, spraying
Generation tank and negative pressure bottle, the bottom of the retort are connected with the top of negative pressure bottle by downspout, the spraying generation tank
It is connected with negative pressure bottle by stalk and communicating pipe, the stalk is drawn by the bottom of spraying generation tank, extends to negative pressure
The bottom of bottle, communicating pipe by the Base top contact of negative pressure bottle, extend to the top of spraying generation tank, the spraying generation tank
Side wall is also communicated with the injection spray pipe extended in retort；The middle part of the stalk is equipped with liquid pump；
Wherein, the retort and the distance of negative pressure bottle are 30~40cm；Liquid level and injection spray pipe top in the spraying generation tank
The distance between be less than 12cm.
2. a kind of application method of atomizing plant cultivation reactor as described in claim 1, it is characterised in that：Including as follows
Step：
In an aseptic environment, fresh fluid nutrient medium is injected into retort first, while opens liquid pump, control pump speed is
0.5~1mL/s；When the liquid level in negative pressure bottle and spraying generating bottle is 2/3rds of bottle height, fresh medium injection
Operation stops；Then germ-free plant material is transferred in culture tank, and is 0.05~0.1mL/s by pump speed fixed setting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510249731.4A CN104877906B (en) | 2015-05-15 | 2015-05-15 | Atomizing plant cultivation reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510249731.4A CN104877906B (en) | 2015-05-15 | 2015-05-15 | Atomizing plant cultivation reactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104877906A CN104877906A (en) | 2015-09-02 |
| CN104877906B true CN104877906B (en) | 2018-07-03 |
Family
ID=53945587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510249731.4A Expired - Fee Related CN104877906B (en) | 2015-05-15 | 2015-05-15 | Atomizing plant cultivation reactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104877906B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7270980B2 (en) * | 2020-02-17 | 2023-05-11 | ヤマト電機資産管理株式会社 | Plant curing system |
| US11299700B1 (en) | 2021-02-19 | 2022-04-12 | Acequia Biotechnology, Llc | Bioreactor containers and methods of growing hairy roots using the same |
| CN114027164A (en) * | 2021-11-04 | 2022-02-11 | 南宁学院 | Edible plant incubator of keeping in |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2791115B2 (en) * | 1989-07-12 | 1998-08-27 | 日東電工株式会社 | Gas phase culture equipment |
| CN101457205B (en) * | 2009-01-08 | 2011-11-16 | 上海交通大学 | Siphoning type intermittent spraying bioreactor |
| CN103031251B (en) * | 2012-12-07 | 2015-01-14 | 上海交通大学 | Multifunctional plant excised root culture bioreactor system |
-
2015
- 2015-05-15 CN CN201510249731.4A patent/CN104877906B/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN104877906A (en) | 2015-09-02 |
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