CN104862252B - A kind of Te Jila Bacillus strains for producing surfactant - Google Patents
A kind of Te Jila Bacillus strains for producing surfactant Download PDFInfo
- Publication number
- CN104862252B CN104862252B CN201510262600.XA CN201510262600A CN104862252B CN 104862252 B CN104862252 B CN 104862252B CN 201510262600 A CN201510262600 A CN 201510262600A CN 104862252 B CN104862252 B CN 104862252B
- Authority
- CN
- China
- Prior art keywords
- bacterial strain
- surfactant
- bacillus
- fjat
- jila
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000004094 surface-active agent Substances 0.000 title claims abstract description 29
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 16
- 230000001580 bacterial effect Effects 0.000 claims abstract description 42
- 241000315694 Bacillus tequilensis Species 0.000 claims abstract description 7
- 244000005700 microbiome Species 0.000 claims abstract description 5
- 241000894006 Bacteria Species 0.000 claims description 15
- 244000144987 brood Species 0.000 claims 1
- 238000004321 preservation Methods 0.000 claims 1
- 231100000252 nontoxic Toxicity 0.000 abstract description 4
- 230000003000 nontoxic effect Effects 0.000 abstract description 4
- 239000000463 material Substances 0.000 abstract description 3
- 231100001231 less toxic Toxicity 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 description 11
- 239000007788 liquid Substances 0.000 description 11
- 239000001963 growth medium Substances 0.000 description 9
- 238000000855 fermentation Methods 0.000 description 8
- 230000004151 fermentation Effects 0.000 description 8
- 238000000605 extraction Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 239000003876 biosurfactant Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 240000002853 Nelumbo nucifera Species 0.000 description 3
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 3
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241000235342 Saccharomycetes Species 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 229910052564 epsomite Inorganic materials 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000011265 semifinished product Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 244000030795 Annona lutescens Species 0.000 description 1
- 235000005288 Annona lutescens Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 108010028921 Lipopeptides Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229920001131 Pulp (paper) Polymers 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- FHNINJWBTRXEBC-UHFFFAOYSA-N Sudan III Chemical compound OC1=CC=C2C=CC=CC2=C1N=NC(C=C1)=CC=C1N=NC1=CC=CC=C1 FHNINJWBTRXEBC-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000011437 continuous method Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000000401 methanolic extract Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of Te Jila Bacillus strains for producing surfactant, the bacterial strain is Te Jila bacillus (Bacillus tequilensis) bacterial strain FJAT 14262a, China Committee for Culture Collection of Microorganisms's common micro-organisms center was preserved on 04 23rd, 2015, deposit number is CGMCC No.10735.The present invention has widened the source of environmentally friendly, nontoxic/less toxic and low cost of material Bio-surface active, in addition, surfactant caused by bacterial strain FJAT 14262a has the characteristics of significantly reducing interfacial tension.
Description
【Technical field】
The invention belongs to microorganism field, and in particular to a kind of Te Jila Bacillus strains for producing surfactant.
【Background technology】
Surfactant is the important raw material of industry, and it has oleophylic hydrophilic radical, aligns in solution surface, can
The surface tension of solvent is significantly reduced, there is the effects such as emulsification, scattered, solubilising, decontamination, be widely used in industrial circle, claimed
For " industrial monosodium glutamate ".
Surfactant typically has point of biosurfactant and chemical surfactant, wherein biosurfactant
In addition to the functions such as reduction surface tension, stable emulsion and foaming with chemical surfactant, also there is low toxicity, easily drop
Solve, be environment-friendly, there is good chemical stability and heat endurance.Biosurfactant can use agricultural wastes for
Raw material, consumption of the synthetic surfactant to the energy is alleviated significantly.Chemical table is substituted with biosurfactant at present
Face activating agent turns into study hotspot.Biosurfactant can be applicable to agricultural, cosmetics, medicine, detergent, personal nursing production
Product, food processing, weaving manufacture, laundry article, metal processing and processing, paper pulp and paper process and paint industry.
And bacillus is distributed widely in the environment such as water body, soil, animal and plant body, air, particularly in some extreme rings
It is the main producing strains of surfactant in border, the exploration for producing surfactant bacillus is friendly to development environment, nontoxic/low
The low Bio-surface active of poison, cost of material is significant.
【The content of the invention】
The technical problems to be solved by the invention are to provide a kind of Te Jila bacillus for producing surfactant
(Bacillus tequilensis) bacterial strain FJAT-14262a, the Te Jila bacillus (Bacillus tequilensis)
Bacterial strain FJAT-14262a was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on 04 23rd, 2015
The heart, address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and deposit number is CGMCC No.10735
The present invention is that solve above-mentioned technical problem by the following technical programs：
This laboratory separates from the wild gold lotus rhizosphere soil of Nanping City in Fujian province Guangze County and passes through blood plate, oil extraction
The obtained bacterial strain of circle screening, is measured and sequence alignment analysis to the 16S rDNA sequences of the bacterial strain, is finally identified as spy
Base draws a bacterial strain for bacillus Pseudomonas.
The beneficial effects of the present invention are：Provide a kind of Te Jila bacillus that can produce surfactant
(Bacillus tequilensis) bacterial strain FJAT-14262a, so as to widened it is environmentally friendly, nontoxic/low toxicity and raw material into
The source of this low Bio-surface active, is significantly reduced in addition, surfactant caused by bacterial strain FJAT-14262a has
The characteristics of interfacial tension.
【Brief description of the drawings】
The utility model will be further described in conjunction with the embodiments with reference to the accompanying drawings.
Fig. 1 is 1.5% agarose gel electrophoresis figure of PCR primer in the present invention.
Fig. 2 is the infrared spectrogram of the surfactant of bacterial strain FJAT-14262a productions of the present invention.
【Embodiment】
Te Jila bacillus (Bacillus tequilensis) bacterial strain FJAT-14262a is from Fujian Province in the present invention
Separated in the wild gold lotus rhizosphere soil of Nanping City Guangze County and screen obtained bacterial strain, by the fermentation for isolating and purifying the bacterial strain
Liquid energy enough obtains surfactant, i.e., the bacterial strain can produce surfactant.
First, bacterial strain FJAT-14262a separation and screening
(1) collection of sample：Take Fujian Province Guangze County wild gold lotus rhizosphere soil to be fitted into collection bag, be placed in room temperature
Under, it is standby；
(2) isolated strains：Earth 10g fetch earth in 90mL sterilized waters, 1mL is fully drawn after vibration and carries out gradient dilution, choosing
It is 10 with dilution factor-1, 10-2, 10-3；Then dilution is respectively coated on bacteria culture media flat board, 3- is cultivated at 30 DEG C
5d, the bacterium colony for cultivating acquisition is inoculated on bacteria culture media flat board using continuous method of scoring afterwards, and training is purified at 30 DEG C
48h is supported, obtains pure bacterial strain；
(3) blood plate primary dcreening operation：According to following operation preparation primary dcreening operation culture medium：Added in per 1L deionized waters peptone 22g,
Sodium chloride 5g, OX-heart powder 3g, soluble starch 1.0g, agar 13g are prepared, and pH 7.3；The primary dcreening operation culture prepared
After base is cooled to 55 DEG C -50 DEG C, 50-100g degreasing sheep blood is added, mixes the culture dish for being poured into sterilizing；Then separating obtained
Pure bacterial strain is inoculated in primary dcreening operation culture medium in culture dish using point connection, after cultivating 3-5d at 15 DEG C, selects the bacterium of production transparent circle
Strain is primary dcreening operation bacterial strain；
(4) bacterial strain secondary screening：Primary dcreening operation bacterial strain is placed on bacteria culture media cultivate bacterial strain single bacterium colony, then bacterial strain
Single bacterium colony is inoculated in seed fluid nutrient mediums of saccharomycete, and is cultivated under 30 DEG C, 200rpm, cultivates to exponential phase of growth OD600Reach 0.6
When terminate；Then it is seeded to 5% inoculum concentration in liquid fermentation medium, 48h is cultivated under 30 DEG C, 200rpm to ferment
Liquid, zymotic fluid is removed into thalline with 10000rpm centrifugations 15min, it is zymotic fluid bacterium solution to retain supernatant, stand-by；By 1mL through Soviet Union
The atoleine of the dyeing of pellet III is added dropwise in 30mL water, and after tonyred completely after liquid level expansion, 1mL zymotic fluids bacterium solution is added dropwise
It is control with initial liquid fermentation medium (liquid fermentation medium of i.e. non-inoculating strain single bacterium colony) to liquid level center,
Whether observation oil extraction circle forms, and observes and measures the diameter of oil extraction circle, selects the big bacterium colony of oil extraction circle and carry out purifying culture, in order to
Convenient description, will purify the Strain Designation obtained by cultivating is bacterial strain FJAT-14262a.
2nd, bacterial strain FJAT-14262a identification
The base that extraction aimed strain is bacterial strain FJAT-14262a is operated with reference to extracts kit (generay bitehch)
Because of a group DNA；Shanghai Bo Shang Bioisystech Co., Ltd is entrusted to utilize primer (such as SEQ ID NO of DNA synthesizer synthesis：1st, 2 institute
Show)：27F：5’-AGAGTTTGATCCTGGCTCAG-3’；1492R：5’-ACGGCTACCTTGTTACGACT-3’.
Using the obtained bacterial strain FJAT-14262a of extraction genomic DNA as template, and with above-mentioned primer (27F/
1492R) its 16S rDNA gene V6~V8 variable region is expanded for primer pair PCR:
PCR reaction systems (25 μ L)：2.5 10 × Buffer of μ L, 0.5 μ L 10mM dNTP, 1 μ L primers (27F), 1 μ L draw
Thing (1492R), 0.3 μ L (5U/ μ L) Taq enzyme and 1 μ L DNA profilings；
PCR response procedures：94 DEG C of pre-degeneration 5min；Then 94 DEG C of denaturation 30s, 55 DEG C of annealing 45s, 72 DEG C of extension 1min
30s, totally 35 circulations；Last 72 DEG C of extensions 10min.
PCR primer product detection and sequencing analysis：Take 2 μ L PCR primers, point sample in 1.5% Ago-Gel, with
100bp Marker examine as standard molecular weight, 100V voltages, electrophoresis 40min, EB dyeing, i.e. progress gel electrophoresis separation,
Electrophoretic separation assay as shown in figure 1, and by PCR primer commission Shanghai Bo Shang Bioisystech Co., Ltd be sequenced, obtain
The sequence arrived is as shown in SEQ ID NO.3.It is right on the EZtaxon-e.ezbiocloud.net of website to be compared in South Korea's bacterial sequences
After bacterial strain FJAT-14262a 16S rRNA sequences are compared, confirm that bacterial strain FJAT-14262a belongs on taxology
In Te Jila bacillus (Bacillus tequilensis) Pseudomonas.
3rd, bacterial strain FJAT-14262a produces the experiment of surfactant
Bacterial strain FJAT-14262a is placed on bacteria culture media cultivate bacterial strain single bacterium colony, then the list of bacterial strain
Colony inoculation is cultivated in seed fluid nutrient mediums of saccharomycete under 30 DEG C, 200rpm, is cultivated to exponential phase of growth OD600When reaching 0.6
Terminate；Then it is seeded to 5% inoculum concentration in liquid fermentation medium, 48h is cultivated under 30 DEG C, 200rpm and obtains zymotic fluid,
Zymotic fluid is removed into thalline with 10000rpm centrifugations 15min, retains supernatant；Gained supernatant is adjusted using 6mol/L HCl
PH to 2.0, now there is white flock precipitate, 4 DEG C stand overnight；Next day collects precipitation with 10000rpm centrifugations 20min, uses pH
2.0 HCl is washed 3 times；Precipitation is extracted 3 times with proper amount of methanol after washing, methanol extract is placed concentration is dried under nitrogen evaporator,
Until the semifinished product of only surplus solid matter, as surfactant, weighs and calculates yield.Surveyed using Fourier transform infrared spectroscopy
Chemical bond and functional group (Nicolet 380, Thermo, USA) in fixed gained surfactant, specifically, take 0.1mg surfaces
The semifinished product of activating agent is placed in 1.0g KBr and is well mixed, and is laminated under 7500kg tablet press machine, determines wave-length coverage
400-4000cm-1, it is as shown in Figure 2 to determine gained infrared spectrogram；Measurement result and Infra-red Absorption Frequency database are compared
It is right, then infer that surfactant caused by bacterial strain FJAT-14262a of the present invention is lipopeptide surfactant.
4th, the surface tension test of bacterial strain FJAT-14262a zymotic fluids is tested
Hair is made in the zymotic fluid preparation manipulation process produced with reference to above-mentioned bacterial strains FJAT-14262a in the experiment of surfactant
Zymotic fluid, gained zymotic fluid is removed into thalline with 10000rpm centrifugations 15min, retains supernatant, it is stand-by；20 DEG C of test temperature and
The surface tension of liquid fermentation medium and supernatant is determined under other conditions are equal respectively；Measure learns, liquid fermentation and culture
The surface tension value of base is 74.1mN/m, and the surface tension value of supernatant is 32.7mN/m, so as to show, bacterial strain of the present invention
The surfactant that FJAT-14262a can not only be produced, and caused surfactant can significantly reduce interfacial tension.
In addition, it is necessary to explanation, as follows in the component of the involved each culture medium of invention：The group of bacteria culture media flat board
Divide and be：Beef extract 0.3%, peptone 1%, sodium chloride 0.5%, distilled water are prepared, pH 7.2；The seed Liquid Culture
The component of base：Glucose 0.5%, beef extract 0.3%, peptone 1%, MgSO4·7H2O 0.02%, pH 7.2；Liquid fermentation
Culture medium：Glucose 5%, peptone 0.6%, KH2PO40.35%, K2HPO4·3H2O 0.24%, MgSO4·7H2O
0.1%, CaCl20.0005%, pH 7.0.
To sum up, the invention provides a kind of Te Jila bacillus (Bacillus that can produce surfactant
Tequilensis) bacterial strain FJAT-14262a, so as to widen environmentally friendly, nontoxic/less toxic and low cost of material biology
The source of surface-active, in addition, surfactant caused by bacterial strain FJAT-14262a, which has, significantly reduces interfacial tension
Feature.
Claims (1)
- A kind of 1. Te Jila Bacillus strains for producing surfactant, it is characterised in that：The bacterial strain is Te Jila brood cell's bar Bacterium (Bacillus tequilensis) bacterial strain FJAT-14262a, Chinese microorganism strain was preserved on 04 23rd, 2015 Preservation administration committee common micro-organisms center, deposit number are CGMCC No.10735.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510262600.XA CN104862252B (en) | 2015-05-22 | 2015-05-22 | A kind of Te Jila Bacillus strains for producing surfactant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510262600.XA CN104862252B (en) | 2015-05-22 | 2015-05-22 | A kind of Te Jila Bacillus strains for producing surfactant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104862252A CN104862252A (en) | 2015-08-26 |
| CN104862252B true CN104862252B (en) | 2018-01-19 |
Family
ID=53908433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510262600.XA Active CN104862252B (en) | 2015-05-22 | 2015-05-22 | A kind of Te Jila Bacillus strains for producing surfactant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104862252B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105695368B (en) * | 2016-04-11 | 2019-03-22 | 江苏省农业科学院 | One plant of Te Jila bacillus and its application |
| CN108277186B (en) * | 2018-04-12 | 2020-07-14 | 扬州大学 | Bacillus licheniformis as pesticide surfactant and application thereof |
| CN115975878B (en) * | 2022-12-15 | 2023-07-07 | 鹤山市新的生物制品有限公司 | Bacillus tertiarygenus strain and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719379A (en) * | 2012-06-15 | 2012-10-10 | 江南大学 | Bacillus tequilensis and application thereof |
| CN102757994A (en) * | 2011-09-21 | 2012-10-31 | 大庆沃太斯化工有限公司 | Industrial production method of lipopeptide bio-surfactant |
-
2015
- 2015-05-22 CN CN201510262600.XA patent/CN104862252B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102757994A (en) * | 2011-09-21 | 2012-10-31 | 大庆沃太斯化工有限公司 | Industrial production method of lipopeptide bio-surfactant |
| CN102719379A (en) * | 2012-06-15 | 2012-10-10 | 江南大学 | Bacillus tequilensis and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| "一株高效表面活性剂产生菌的筛选及其活性产物的结构与性质";肖兵等;《生态学杂志》;20130315;第32卷(第3期);正文第782-783页左栏结果分析2.1-2.3 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104862252A (en) | 2015-08-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110257310B (en) | A kind of vinyon efficient degrading bacteria and its separating screening method and application | |
| CN102286412B (en) | Bacillus velezensis and application thereof in tomato blight antagonism | |
| CN105647839B (en) | A kind of desert Oligotrophic bacteria DOB150 and the application in fixing the sand | |
| CN105670964B (en) | Bacillus atrophaeus BsR05 strain and application thereof | |
| CN102321554B (en) | Bacillus cereus and its application of being as plant growth-promoting rhizobacteria | |
| CN104862252B (en) | A kind of Te Jila Bacillus strains for producing surfactant | |
| CN106978366A (en) | A kind of mix bacterium agent and its application in compost maturity is promoted | |
| CN107058177A (en) | A kind of hot denitrification ground bacillus bacterial strain TB62 and its application in compost maturity is promoted | |
| CN106957807A (en) | A kind of lichem bacillus strain TA65 and its application in compost maturity is promoted | |
| CN104877926A (en) | Anti-Cotinus coggygria oxysporum strain and use thereof | |
| CN109868236A (en) | One plant of Jie meter La series bacillus, its tunning and preparation method and application | |
| CN112746039A (en) | Bacillus subtilis subspecies deserticola strain and application thereof | |
| CN103371041A (en) | Method for screening bio-control bacteria of cucumber fusarium wilt | |
| CN102021131B (en) | Bacillus licheniformis strain and application thereof | |
| CN103173386A (en) | Bio-control strain G1 for preventing and controlling pepper phytophthora blights and applications thereof | |
| CN106834177B (en) | One plant of cud bacterium and its application | |
| CN104630103B (en) | A kind of microbial bacterial agent and its preparation method and application | |
| CN105154353B (en) | A kind of bacillus subtilis and its application in greenhouse soil remediation | |
| CN104087526B (en) | A kind of bacillus licheniformis is utilized to control the method for earthy in white wine | |
| CN104004693B (en) | A kind of Bacillus pumilus and the application of earthy in controlling Chinese liquor thereof | |
| CN106591173A (en) | Bacillus flexus HL-37 capable of activating soil heavy metal cadmium, and applications thereof | |
| CN107217016B (en) | One plant has the endophytic Bacillus bacterial strain ZY122 for inhibiting rice sheath blight disease and its application | |
| CN109182189A (en) | The oxidation microbacterium and its application that one plant height produces | |
| Han et al. | Denitratimonas tolerans gen. nov., sp. nov., a denitrifying bacterium isolated from a bioreactor for tannery wastewater treatment | |
| CN107760629A (en) | A kind of Methylotrophic bacillus B18 and its wettable powder and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20231011 Address after: 530007, 1st to 5th floors, A-2, Nanning Biotechnology Center, No. 18 Xinji Road, Nanning City, Guangxi Zhuang Autonomous Region Patentee after: NANNING HARWORLD BIOTECHNOLOGY Co.,Ltd. Address before: 350000 No. 54, 247, Fuzhou, Fujian Patentee before: AGRICULTURAL BIORESOURCES INSTITUTE OF FUJIAN ACADEMY OF AGRICULTURAL SCIENCES |