CN104862252A - Bacillus tequilensis strain for producing surfactant - Google Patents
Bacillus tequilensis strain for producing surfactant Download PDFInfo
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- CN104862252A CN104862252A CN201510262600.XA CN201510262600A CN104862252A CN 104862252 A CN104862252 A CN 104862252A CN 201510262600 A CN201510262600 A CN 201510262600A CN 104862252 A CN104862252 A CN 104862252A
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- 241000315694 Bacillus tequilensis Species 0.000 title claims abstract description 11
- 239000004094 surface-active agent Substances 0.000 title abstract description 9
- 230000001580 bacterial effect Effects 0.000 claims description 37
- 239000013543 active substance Substances 0.000 claims description 23
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 13
- 244000005700 microbiome Species 0.000 claims description 3
- 239000002994 raw material Substances 0.000 abstract description 6
- 231100000252 nontoxic Toxicity 0.000 abstract description 4
- 230000003000 nontoxic effect Effects 0.000 abstract description 4
- 230000009467 reduction Effects 0.000 abstract description 2
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 24
- 241000894006 Bacteria Species 0.000 description 12
- 239000002609 medium Substances 0.000 description 10
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 239000003876 biosurfactant Substances 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000001888 Peptone Substances 0.000 description 4
- 108010080698 Peptones Proteins 0.000 description 4
- 231100000053 low toxicity Toxicity 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 235000019319 peptone Nutrition 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108020004465 16S ribosomal RNA Proteins 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 2
- 239000006161 blood agar Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 235000005288 Annona lutescens Nutrition 0.000 description 1
- 244000030795 Annona lutescens Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229920001131 Pulp (paper) Polymers 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- JBTHDAVBDKKSRW-UHFFFAOYSA-N chembl1552233 Chemical compound CC1=CC(C)=CC=C1N=NC1=C(O)C=CC2=CC=CC=C12 JBTHDAVBDKKSRW-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000011437 continuous method Methods 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 238000000247 postprecipitation Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 229940073450 sudan red Drugs 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Abstract
The invention provides a bacillus tequilensis strain for producing a surfactant. The strain is a FJAT-14262a (bacillus tequilensis) strain and is preserved in the CGMCC (China General Microbiological Culture Collection Center) on 23rd, April, 2015, and the preservation number is CGMCC No. 10735. According to the bacillus tequilensis strain, the source of a biological surfactant which is environment-friendly, non-toxic/low-toxic and low in raw material cost is broadened; besides, the surfactant produced by the FJAT-14262a strain has the characteristic of remarkable reduction of interfacial tension.
Description
[technical field]
The invention belongs to microorganism field, be specifically related to a kind of Te Jila Bacillus strain producing tensio-active agent.
[background technology]
Tensio-active agent is important industrial raw material, and it has oleophylic hydrophilic radical, aligns in solution surface, significantly can reduce the surface tension of solvent, there is the effects such as emulsification, dispersion, solubilising, decontamination, be widely used in industrial circle, be called as " industrial monosodium glutamate ".
Tensio-active agent generally have bio-surfactant and chemical surfactant point, wherein bio-surfactant is except having the functions such as the reduction surface tension of chemical surfactant, stable emulsion and foaming, also has low toxicity, easily degraded, environmental friendliness, has good chemical stability and thermostability.Bio-surfactant can adopt agricultural wastes to be raw material, greatly alleviates the consumption of synthetic surfactant to the energy.Substituting chemical surfactant with bio-surfactant becomes study hotspot at present.Bio-surfactant can be applicable to agricultural, makeup, medicine, washing composition, personal care product, food-processing, weaving manufacture, laundry article, metal treatment and process, paper pulp and paper process and paint industry.
And bacillus is distributed widely in the environment such as water body, soil, animal and plant body, air, particularly in some extreme environments, be the main producing strains of tensio-active agent, the exploration of producing tensio-active agent bacillus is significant to development environment close friend, nontoxic/low toxicity, Bio-surface active that raw materials cost is low.
[summary of the invention]
Technical problem to be solved by this invention is to provide a kind of Te Jila Bacillus strain FJAT-14262a (Bacillus tequilensis) producing tensio-active agent, this Te Jila Bacillus strain FJAT-14262a (Bacillus tequilensis) was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 04 23rd, 2015, address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and deposit number is CGMCC No.10735
The present invention solves the problems of the technologies described above by the following technical programs:
This laboratory is separated and screens by blood agar, oil extraction circle the bacterial strain obtained from the wild Herba Anoectochili roxburghii rhizosphere soil in Guangze County, Nanping City in Fujian province, the 16S rDNA sequence of this bacterial strain is measured and sequence alignment analysis, finally identifies that it is a bacterial strain of Te Jila genus bacillus Pseudomonas.
Beneficial effect of the present invention is: provide a kind of Te Jila bacterial strain of bacillus FJAT-14262a (Bacillus tequilensis) that can produce tensio-active agent, thus widened environmentally friendly, nontoxic/low toxicity and the source of the low Bio-surface active of raw materials cost, in addition, the tensio-active agent that this bacterial strain FJAT-14262a produces has the advantages that significantly reduce interfacial tension.
[accompanying drawing explanation]
The invention will be further described in conjunction with the embodiments with reference to the accompanying drawings.
Fig. 1 is 1.5% agarose gel electrophoresis figure of PCR primer in the present invention.
Fig. 2 is the infrared spectrogram of the tensio-active agent that bacterial strain FJAT-14262a of the present invention produces.
[embodiment]
In the present invention, Te Jila Bacillus strain FJAT-14262a (Bacillus tequilensis) is separated and screens the bacterial strain obtained from the wild Herba Anoectochili roxburghii rhizosphere soil in Guangze County, Nanping City in Fujian province, can obtain tensio-active agent by the fermented liquid of this bacterial strain of separation and purification, namely this bacterial strain can produce tensio-active agent.
One, the isolation and screening of this bacterial strain FJAT-14262a
(1) collection of sample: get the wild Herba Anoectochili roxburghii rhizosphere soil in Guangze County, Fujian Province and load in collection bag, under being placed in room temperature, for subsequent use;
(2) isolated strains: fetch earth earth 10g in 90mL sterilized water, fully draws 1mL and carries out gradient dilution, select extent of dilution to be 10 after vibration
-1, 10
-2, 10
-3; Then diluent being coated respectively on bacteria culture medium flat board, at 30 DEG C, cultivate 3-5d, adopt continuous method of scoring to be inoculated on bacteria culture medium flat board by cultivating the bacterium colony obtained afterwards, and purifying cultivating 48h at 30 DEG C, obtains pure bacterial strain;
(3) blood agar primary dcreening operation: prepare primary dcreening operation substratum according to following operation: add peptone 22g, sodium-chlor 5g in every 1L deionized water, OX-heart powder 3g, Zulkovsky starch 1.0g, agar 13g prepare, and pH 7.3; After the primary dcreening operation substratum prepared is cooled to 55 DEG C-50 DEG C, add 50-100g degreasing sheep blood, mixing is poured into the culture dish of sterilizing; Then adopt some connection to be inoculated in primary dcreening operation substratum in culture dish separating obtained pure bacterial strain, cultivate 3-5d at 15 DEG C after, select the bacterial strain producing transparent circle and be primary dcreening operation bacterial strain;
(4) bacterial strain sieves again: be placed in by primary dcreening operation bacterial strain and bacteria culture medium cultivate to obtain single bacterium colony of bacterial strain, then single colony inoculation of bacterial strain in seed liquid nutrient medium, and in 30 DEG C, cultivate under 200rpm, be cultured to OD exponential phase of growth
600terminate when reaching 0.6; Then be seeded in liquid fermentation medium with the inoculum size of 5%, in 30 DEG C, cultivate 48h under 200rpm and obtain fermented liquid, fermented liquid is removed thalline with the centrifugal 15min of 10000rpm, retains supernatant liquor and fermented liquid bacterium liquid, stand-by; 1mL is dripped in 30mL water through the whiteruss that oil red dyes, until Sudan red completely after liquid level launches, 1mL fermented liquid bacterium drop is added to liquid level central authorities, with initial liquid fermentation medium (i.e. the liquid fermentation medium of non-inoculating strain list bacterium colony) for contrast, observe oil extraction circle whether to be formed, observe and measure the diameter of oil extraction circle, select the large bacterium colony of oil extraction circle and carry out purifying cultivation, for convenience of description, Strain Designation purifying being cultivated gained is bacterial strain FJAT-14262a.
Two, the qualification of this bacterial strain FJAT-14262a
With reference to the genomic dna extracting test kit (generay bitehch) operation extraction aimed strain and bacterial strain FJAT-14262a; The primer (as shown in SEQ ID NO:1,2) of entrusting Shanghai Bo Shang Bioisystech Co., Ltd to utilize DNA synthesizer to synthesize: 27F:5 '-AGAGTTTGATCCTGGCTCAG-3 '; 1492R:5 '-ACGGCTACCTTGTTACGACT-3 '.
To extract the genomic dna of the bacterial strain FJAT-14262a obtained for template, and with above-mentioned primer (27F/1492R) for its 16S rDNA gene V6 ~ V8 variable region of primer pair pcr amplification:
PCR reaction system (25 μ L): the Taq enzyme of 2.5 μ L 10 × Buffer, 0.5 μ L 10mM dNTP, 1 μ L primer (27F), 1 μ L primer (1492R), 0.3 μ L (5U/ μ L) and 1 μ L DNA profiling;
PCR response procedures: 94 DEG C of denaturation 5min; Then 94 DEG C of sex change 30s, 55 DEG C of annealing 45s, 72 DEG C extend 1min 30s, totally 35 circulations; Last 72 DEG C extend 10min.
PCR primer product detects and sequencing analysis: get 2 μ L PCR primer, point sample is in the sepharose of 1.5%, using 100bp Marker as standard molecular weight, 100V voltage, electrophoresis 40min, EB dye, namely carry out gel electrophoresis and be separated inspection, PCR primer as shown in Figure 1, and entrusts Shanghai Bo Shang Bioisystech Co., Ltd to check order by electrophoretic separation assay, and the sequence obtained is as shown in SEQ ID NO.3.Korea S's bacterial sequences comparison website EZtaxon-e.ezbiocloud.net after comparing to the 16S rRNA sequence of bacterial strain FJAT-14262a, confirm that this bacterial strain FJAT-14262a belongs to Te Jila genus bacillus (Bacillus tequilensis) Pseudomonas on taxonomy.
Three, this bacterial strain FJAT-14262a produces the test of tensio-active agent
This bacterial strain FJAT-14262a is placed in and bacteria culture medium cultivates to obtain single bacterium colony of bacterial strain, then single colony inoculation of bacterial strain in seed liquid nutrient medium, and in 30 DEG C, cultivate under 200rpm, be cultured to OD exponential phase of growth
600terminate when reaching 0.6; Then be seeded in liquid fermentation medium with the inoculum size of 5%, in 30 DEG C, cultivate 48h under 200rpm and obtain fermented liquid, fermented liquid is removed thalline with the centrifugal 15min of 10000rpm, retains supernatant liquor; Gained supernatant liquor adopts the HCl of 6mol/L to regulate pH to 2.0, now occurs white flock precipitate, 4 DEG C of hold over night; Next day, with the centrifugal 20min collecting precipitation of 10000rpm, washs 3 times with the HCl of pH 2.0; The proper amount of methanol extracting 3 times of washing postprecipitation, dry concentrated under methyl alcohol extract being placed Nitrogen evaporator, until only remain solid matter, be the raw product of tensio-active agent, calculating output of weighing.Adopt the chemical bond in Fourier transform infrared spectroscopy mensuration gained tensio-active agent and functional group (Nicolet 380, Thermo, USA), particularly, the raw product getting 0.1mg tensio-active agent is placed in 1.0g KBr and mixes, laminate under the tabletting machine of 7500kg, total range of wavelength 400-4000cm
-1, measure gained infrared spectrogram as shown in Figure 2; Measurement result and Infra-red Absorption Frequency database are compared, then infers that the tensio-active agent that bacterial strain FJAT-14262a of the present invention produces is fat peptid-based surfactant.
Four, this bacterial strain FJAT-14262a fermented liquid measurement of surface tension test
The fermented liquid preparation manipulation process of producing with reference to above-mentioned bacterial strains FJAT-14262a in the test of tensio-active agent obtains fermented liquid, gained fermented liquid is removed thalline with the centrifugal 15min of 10000rpm, retains supernatant liquor, stand-by; The surface tension of liquid fermentation medium and supernatant liquor is measured respectively under probe temperature 20 DEG C and other condition are equal; Mensuration is learnt, the surface tension value of liquid fermentation medium is 74.1mN/m, and the surface tension value of supernatant liquor is 32.7mN/m, thus shows, the tensio-active agent that bacterial strain FJAT-14262a of the present invention can not only produce, and the tensio-active agent produced significantly can reduce interfacial tension.
In addition, it should be noted that, the component of each substratum involved by invention is as follows: the component of bacteria culture medium flat board is: beef extract 0.3%, peptone 1%, sodium-chlor 0.5%, and distilled water is prepared, pH 7.2; The component of described seed liquid nutrient medium: glucose 0.5%, extractum carnis 0.3%, peptone 1%, MgSO
47H
2o 0.02%, pH 7.2; Liquid fermentation medium: glucose 5%, peptone 0.6%, KH
2pO
40.35%, K
2hPO
43H
2o 0.24%, MgSO
47H
2o 0.1%, CaCl
20.0005%, pH 7.0.
To sum up, the invention provides a kind of Te Jila bacterial strain of bacillus FJAT-14262a (Bacillus tequilensis) that can produce tensio-active agent, thus widened environmentally friendly, nontoxic/low toxicity and the source of the low Bio-surface active of raw materials cost, in addition, the tensio-active agent that this bacterial strain FJAT-14262a produces has the advantages that significantly reduce interfacial tension.
Claims (1)
1. produce a Te Jila Bacillus strain for tensio-active agent, it is characterized in that: described bacterial strain is
Te Jila bacterial strain of bacillus FJAT-14262a (Bacillus tequilensis), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 04 23rd, 2015, deposit number is CGMCC No.10735.
Priority Applications (1)
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|---|---|---|---|
| CN201510262600.XA CN104862252B (en) | 2015-05-22 | 2015-05-22 | A kind of Te Jila Bacillus strains for producing surfactant |
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|---|---|---|---|
| CN201510262600.XA CN104862252B (en) | 2015-05-22 | 2015-05-22 | A kind of Te Jila Bacillus strains for producing surfactant |
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| Publication Number | Publication Date |
|---|---|
| CN104862252A true CN104862252A (en) | 2015-08-26 |
| CN104862252B CN104862252B (en) | 2018-01-19 |
Family
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105695368A (en) * | 2016-04-11 | 2016-06-22 | 江苏省农业科学院 | Tequila bacillus and application thereof |
| CN108277186A (en) * | 2018-04-12 | 2018-07-13 | 扬州大学 | A kind of bacillus licheniformis and its application as pesticide surfactant |
| CN115975878A (en) * | 2022-12-15 | 2023-04-18 | 鹤山市新的生物制品有限公司 | Bacillus tequilensis strain and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719379A (en) * | 2012-06-15 | 2012-10-10 | 江南大学 | Bacillus tequilensis and application thereof |
| CN102757994A (en) * | 2011-09-21 | 2012-10-31 | 大庆沃太斯化工有限公司 | Industrial production method of lipopeptide bio-surfactant |
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2015
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102757994A (en) * | 2011-09-21 | 2012-10-31 | 大庆沃太斯化工有限公司 | Industrial production method of lipopeptide bio-surfactant |
| CN102719379A (en) * | 2012-06-15 | 2012-10-10 | 江南大学 | Bacillus tequilensis and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105695368A (en) * | 2016-04-11 | 2016-06-22 | 江苏省农业科学院 | Tequila bacillus and application thereof |
| CN105695368B (en) * | 2016-04-11 | 2019-03-22 | 江苏省农业科学院 | One plant of Te Jila bacillus and its application |
| CN108277186A (en) * | 2018-04-12 | 2018-07-13 | 扬州大学 | A kind of bacillus licheniformis and its application as pesticide surfactant |
| CN108277186B (en) * | 2018-04-12 | 2020-07-14 | 扬州大学 | Bacillus licheniformis as pesticide surfactant and application thereof |
| CN115975878A (en) * | 2022-12-15 | 2023-04-18 | 鹤山市新的生物制品有限公司 | Bacillus tequilensis strain and application thereof |
| CN115975878B (en) * | 2022-12-15 | 2023-07-07 | 鹤山市新的生物制品有限公司 | Bacillus tertiarygenus strain and application thereof |
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| Publication number | Publication date |
|---|---|
| CN104862252B (en) | 2018-01-19 |
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