CN104862207A - Hanging-bag microalgae photoreactor and hanging-bag microalgae culture system - Google Patents
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Abstract
本发明涉及一种吊袋式微藻光反应器及吊袋式微藻养殖系统。所述吊袋式微藻光反应器包括由端部封闭的透明薄膜袋形成的反应器主体；所述透明薄膜袋的内部空间由至少一条隔断线分割为多空间结构，所述隔断线存在非连续断开段。所述吊袋式微藻养殖系统中包括至少一个所述的吊袋式微藻光反应器。本发明所述的吊袋式微藻光反应器透光性能好，增加了CO2气体在水体中的滞留时间，更有利于微藻培养体系对CO2气体的吸收。所述的吊袋式微藻养殖系统结构更简单，制造和使用成本低廉，可以实现封闭式清洁高品质微藻养殖要求。
The invention relates to a hanging bag type microalgae photoreactor and a hanging bag type microalgae cultivation system. The hanging bag microalgae photoreactor includes a reactor body formed by a transparent film bag with the ends closed; the internal space of the transparent film bag is divided into a multi-space structure by at least one partition line, and the partition line has a discontinuous Disconnect segment. The hanging bag microalgae cultivation system includes at least one hanging bag microalgae photoreactor. The hanging bag type microalgae photoreactor of the invention has good light transmission performance, increases the residence time of CO2 gas in the water body, and is more conducive to the absorption of CO2 gas by the microalgae culture system. The hanging bag type microalgae cultivation system has a simpler structure, low manufacturing and use costs, and can meet the requirements of closed, clean and high-quality microalgae cultivation.
Description
技术领域technical field
本发明涉及一种吊袋式微藻光反应器及吊袋式微藻养殖系统，是一种利用透明薄膜袋实施光合微藻养殖的装置。The invention relates to a hanging bag type microalgae photoreactor and a hanging bag type microalgae cultivation system, which is a device for carrying out photosynthetic microalgae cultivation by using a transparent film bag.
背景技术Background technique
微藻能够利用简单的矿物质营养素，在光合作用（日光和人工照明）的情况下，将CO2和水转化为我们生活需要的各种有机化合物（如色素、蛋白质、氨基酸、维生素和脂肪酸、多糖和矿物质等人体必需的营养成份）。由于藻类具有高效光利用率，以及从周围环境中快速高效吸收营养素（如CO2、氮、磷等）并转化成有机化合物的能力，使藻类具有解决食品短缺、能源危机和CO2减排的巨大经济潜力。Microalgae are able to use simple mineral nutrients to convert CO2 and water into various organic compounds (such as pigments, proteins, amino acids, vitamins and fatty acids, essential nutrients such as polysaccharides and minerals). Due to the high efficiency of light utilization and the ability of algae to quickly and efficiently absorb nutrients (such as CO 2 , nitrogen, phosphorus, etc.) great economic potential.
目前，微藻培养主要有开放式和封闭式两种光生物反应器。其中，封闭式光生物反应器主要有管道式、平板式、柱状气升式、浮式薄膜袋等。这些反应器不仅制造成本高，而且需要占用大量耕地或养殖水面，形成与农作物或水产品争地争水的局面。我国农业一直有农作物间种，即利用植物的不同高度、采收时间不同错时或分层种植，以提高单位面积产值和养殖空间的传统。吊袋式微藻光反应器有利于实现微藻的空中养殖，实现非占地养殖产业发展模式。At present, there are mainly two types of photobioreactors for microalgae cultivation: open and closed. Among them, closed photobioreactors mainly include pipeline type, flat plate type, columnar airlift type, floating film bag and so on. These reactors are not only expensive to manufacture, but also need to occupy a large amount of arable land or aquaculture water surface, forming a situation of competing for land and water with crops or aquatic products. my country's agriculture has always had the tradition of interplanting crops, that is, using different heights of plants, different harvesting times, or planting in layers to increase the output value per unit area and the breeding space. The hanging bag microalgae photoreactor is beneficial to realize the aerial cultivation of microalgae and realize the development mode of non-occupancy aquaculture industry.
CN102086437A公开了薄膜挂袋式单胞藻光生物反应器及其制作方法，提出了一种底部带有倒圆锥状气升部的中空透明柱状体，也加设了悬挂结构。这种结构实质是对大直径单柱光反应器的一种简化，柱状体光反应器气体停留时间短，大直径的柱状结构导致中心部位光照不足，形成养殖暗区，养殖暗区降低了柱状光反应器养殖效率，加大光生物反应器重量，光生物反应器不得不加装加强结构，造成反应器制造成本提高，光反应器透光性不好。CN102086437A discloses a film-hanging bag-type single-celled algae photobioreactor and a manufacturing method thereof, and proposes a hollow transparent columnar body with an inverted conical air-lift portion at the bottom, and a suspension structure is also added. This structure is essentially a simplification of the large-diameter single-column photoreactor. The gas residence time of the columnar photoreactor is short, and the large-diameter columnar structure leads to insufficient light in the center, forming a dark area for breeding, which reduces the columnar shape. The cultivation efficiency of the photoreactor increases the weight of the photobioreactor, and the photobioreactor has to be equipped with a strengthening structure, resulting in an increase in the manufacturing cost of the reactor, and the light transmission of the photoreactor is not good.
CN102373150A和CN102373151A分别提出了两种形式的光生物反应器和光生物培养系统。通过在柔性薄膜袋上设置多个隔离点或竖向的线状隔离带，将光生物反应器内的光生物培养液分割成多个相互连通的液体区域。上述两篇现有专利是对目前常用薄膜培养袋横向隔离带结构的改造，与CN102086437A及现有技术相比，解决了大直径单柱光反应器透光和培养物分层问题，但其分割模式由线到点状分割，是两种极端情况，纵向隔离带与气体上升方向一致，反而不利于气体在培养体系的横向搅拌，点状隔离对解决培养气体搅拌与气体分布情况并不理想。CN102373150A and CN102373151A propose two forms of photobioreactor and photobiological cultivation system respectively. By setting multiple isolation points or vertical linear isolation belts on the flexible film bag, the photobiological culture solution in the photobioreactor is divided into a plurality of interconnected liquid areas. The above two existing patents are the transformation of the structure of the transverse isolation zone of the commonly used film culture bag at present. Compared with CN102086437A and the prior art, it solves the problems of light transmission and culture layering of the large-diameter single-column photoreactor, but its division The mode is divided from line to point, which are two extreme cases. The longitudinal isolation zone is consistent with the rising direction of the gas, which is not conducive to the horizontal stirring of the gas in the culture system. The point isolation is not ideal for solving the gas stirring and gas distribution of the culture.
发明内容Contents of the invention
为改善大型柱状薄膜挂袋式光生物反应器及其他现有光生物反应器的问题，提高微藻养殖效果，本发明提出一种吊袋式微藻光反应器及吊袋式微藻养殖系统，在实现微藻的高效空间养殖的同时，改善光反应器光照效果、营养物质的供给并延长CO2气体在养殖水体的滞留时间，以提高水体光合养殖微藻的产量。In order to improve the problems of large columnar film hanging bag photobioreactor and other existing photobioreactors, and improve the effect of microalgae cultivation, the present invention proposes a hanging bag microalgae photoreactor and a hanging bag microalgae cultivation system. While realizing the high-efficiency space cultivation of microalgae, improve the light effect of the photoreactor, the supply of nutrients and prolong the residence time of CO 2 gas in the cultured water body, so as to increase the output of photosynthetic cultured microalgae in the water body.
为达此目的，本发明采用以下技术方案：For reaching this purpose, the present invention adopts following technical scheme:
本发明的目的之一在于提供一种吊袋式微藻光反应器，所述吊袋式微藻光反应器包括由端部封闭的透明薄膜袋形成的反应器主体；所述透明薄膜袋的内部空间由至少一条隔断线分割为多空间结构，所述隔断线存在非连续断开段。One of the objects of the present invention is to provide a hanging bag type microalgae photoreactor, which includes a reactor body formed by a transparent film bag with an end closed; the inner space of the transparent film bag It is divided into multi-space structures by at least one partition line, and the partition line has non-continuous disconnection segments.
本发明所述的吊袋式微藻光反应器在透明薄膜袋上设置至少一条隔断线，同时在隔断线上设置非连续断开段，便于不同隔断区之间的物质交换，加入培养液后，形成若干相互连通的液体区域。在使用时，从底部分布的气体在上升过程中一部分滞留在隔断线内，多余的气体溢出向上移动、层层滞留，强化了培养液的扰动，增加了CO2气体在水体中的滞留时间。The hanging bag microalgae photoreactor according to the present invention is provided with at least one partition line on the transparent film bag, and at the same time, a non-continuous disconnection section is arranged on the partition line to facilitate material exchange between different partition areas. After adding the culture solution, Several interconnected liquid regions are formed. When in use, part of the gas distributed from the bottom stays in the partition line during the ascent process, and the excess gas overflows and moves upward and stays layer by layer, which strengthens the disturbance of the culture medium and increases the residence time of CO2 gas in the water body.
所述隔断线可选择一条、三条、四条、六条等，可由本领域技术人员根据实际情况进行选择。One, three, four, six, etc. can be selected as the partition lines, which can be selected by those skilled in the art according to the actual situation.
所述隔断线由数组并列排布的倒V形、倒U形或W形的线条组成。The partition line is composed of an array of inverted V-shaped, inverted U-shaped or W-shaped lines arranged side by side.
所述透明薄膜袋的上部设有营养液入口和排气口，底部设有进气口和排料口。所述排料口为单独设置于透明薄膜袋底部的单独接口，或与进气口共用同一接口。The upper part of the transparent film bag is provided with a nutrient solution inlet and an exhaust port, and the bottom is provided with an air inlet and a discharge port. The discharge port is a separate interface separately arranged at the bottom of the transparent film bag, or shares the same interface with the air inlet.
所述透明薄膜袋的材料可选择聚乙烯PE薄膜、聚氯乙烯PVC薄膜或聚氨酯PU薄膜等。所述吊袋式微藻光反应器的透光性能好。The material of the transparent film bag can be selected from polyethylene PE film, polyvinyl chloride PVC film or polyurethane PU film. The hanging bag microalgae photoreactor has good light transmission performance.
本发明的目的之二在于提供一种吊袋式微藻养殖系统，所述系统中包括至少一个如上所述的吊袋式微藻光反应器。所述吊袋式微藻养殖系统的其他部件，如输气、输液、排气等具体部件的设计，可由本领域技术人员进行选择，本发明并无特殊限制。The second object of the present invention is to provide a hanging bag microalgae cultivation system, which includes at least one hanging bag microalgae photoreactor as described above. Other components of the hanging bag microalgae culture system, such as the design of specific components such as gas transmission, liquid infusion, and exhaust, can be selected by those skilled in the art, and the present invention is not particularly limited.
以下为本发明所述吊袋式微藻养殖系统的优选形式。The following are preferred forms of the hanging bag microalgae culture system of the present invention.
本发明所述微藻养殖系统主要由输液输气系统、吊袋式微藻光反应器、气体回收系统、培养液回收系统和辅助支架组成。The microalgae breeding system of the present invention is mainly composed of a liquid and gas delivery system, a hanging bag microalgae photoreactor, a gas recovery system, a culture solution recovery system and an auxiliary support.
所述输液输气系统包括营养罐和连入营养罐底部的气源。其主要作用是向吊袋式微藻光反应器中输入营养液和含有CO2的气体。The infusion and air delivery system includes a nutrition tank and an air source connected to the bottom of the nutrition tank. Its main function is to input nutrient solution and gas containing CO2 into the hanging bag microalgae photoreactor.
所述营养罐分为上部的气体空间和下部的液体空间；气体空间连接有输气管，其末端通过输气针头接入透明薄膜袋底部的进气口，并与透明薄膜袋底部的布气软管连接；液体空间连接有输液管，其末端通过输液针头接入透明薄膜袋上部的营养液入口。The nutrition tank is divided into an upper gas space and a lower liquid space; the gas space is connected with a gas delivery pipe, the end of which is connected to the air inlet at the bottom of the transparent film bag through a gas delivery needle, and connected to the air distribution soft tube at the bottom of the transparent film bag. tube connection; the liquid space is connected with an infusion tube, the end of which is connected to the nutrient solution inlet on the upper part of the transparent film bag through the infusion needle.
本发明所述输液输气系统中气源与营养罐连接，输液管入口浸没在营养罐的液体空间，输气管设置在营养罐的气体空间，由气源向营养罐提供压力推动气体和营养液向吊袋式微藻光反应器内流动。In the infusion and gas delivery system of the present invention, the gas source is connected to the nutrition tank, the inlet of the infusion tube is immersed in the liquid space of the nutrition tank, the gas delivery pipe is arranged in the gas space of the nutrition tank, and the gas source provides pressure to the nutrition tank to push gas and nutrient solution Flow into the hanging bag microalgae photoreactor.
所述输气管上设有输气阀。另外，所述输气管上还设有加热器和冷却器，能够实现对输送气体的温度调节，以及吊袋式微藻光反应器培养体系的温度调控。An air delivery valve is provided on the air delivery pipe. In addition, the gas pipeline is also provided with a heater and a cooler, which can realize the temperature adjustment of the transport gas and the temperature control of the hanging bag microalgae photoreactor culture system.
所述输液管上设有输液阀，调节输液阀采用滴定的方式向吊袋式微藻光反应器输送营养液。The infusion tube is provided with an infusion valve, and the infusion valve is adjusted to deliver nutrient solution to the hanging bag microalgae photoreactor by titration.
所述布气软管开有若干微孔，能够保证气体分布均匀。The gas distribution hose is provided with a number of micropores, which can ensure uniform gas distribution.
所述气体回收系统包括依次连接的排气针头、气袋和压气机；所述排气针头与透明薄膜袋上部的排气口连通，压气机与气源连通。The gas recovery system includes an exhaust needle, an air bag and an air compressor connected in sequence; the exhaust needle communicates with the exhaust port on the upper part of the transparent film bag, and the air compressor communicates with the gas source.
气体回收系统连接在透明薄膜袋的排气口上，将送入袋内未被微藻利用而排出的气体，经过气袋和压气机送回气源，实现气体回收再循环利用，解决目前CO2气体在培养液中吸收率较低，未被微藻利用的大量CO2气体直接排放到空气中造成环境污染的问题。The gas recovery system is connected to the exhaust port of the transparent film bag, and the gas that is not used by the microalgae in the bag is sent back to the gas source through the air bag and the compressor to realize gas recovery and recycling, and solve the current CO 2 The absorption rate of gas in the culture solution is low, and a large amount of CO 2 gas that is not utilized by microalgae is directly discharged into the air, causing environmental pollution.
所述培养液回收系统包括依次连接的排料管阀、过滤器及水箱，所述排料管阀与透明薄膜袋底部的排料口连通。所述排料口为设置在透明薄膜袋底部的单独接口或者与透明薄膜袋底部的进气口共用接口，排料管阀用于培养的微藻排出光反应器的转运和收集过程的调控。所述水箱与营养罐连通，用于培养液的回用输送。在水箱内液体静压差不能满足培养液回流营养罐时，在水箱和营养罐之间设置输液泵。The culture solution recovery system includes a discharge pipe valve, a filter and a water tank connected in sequence, and the discharge pipe valve communicates with the discharge port at the bottom of the transparent film bag. The discharge port is a separate interface arranged at the bottom of the transparent film bag or a common interface with the air inlet at the bottom of the transparent film bag, and the discharge pipe valve is used for the transfer and collection process of the cultured microalgae discharged from the photoreactor. The water tank communicates with the nutrient tank and is used for the reuse and transportation of the culture solution. When the static pressure difference of the liquid in the water tank cannot satisfy the return of the culture fluid to the nutrition tank, an infusion pump is arranged between the water tank and the nutrition tank.
所述水箱与输液泵之间设有排液管阀，其作用是调控过滤器滤出培养微藻的水及其回收的营养液向营养罐的输送，并方便设备检修。A drain pipe valve is arranged between the water tank and the infusion pump, and its function is to control the water filtered out by the filter for cultivating microalgae and the nutrient solution recycled to the nutrition tank, and to facilitate equipment maintenance.
所述输液泵与营养罐之间设有输液隔断阀，其作用是防止营养罐内液体向输液泵倒流并方便设备检修。An infusion isolation valve is arranged between the infusion pump and the nutrition tank, and its function is to prevent the liquid in the nutrition tank from flowing back to the infusion pump and to facilitate equipment maintenance.
所述培养液回收系统连接在透明薄膜袋底部的排料口，将透明薄膜袋内培养液滤出微藻后，回收再利用培养液资源，减少目前微藻培养系统对水及营养资源的大量消耗。The culture solution recovery system is connected to the discharge port at the bottom of the transparent film bag, and after the culture solution in the transparent film bag is filtered out of the microalgae, the culture solution resources are recycled and reused, reducing the amount of water and nutrient resources that the current microalgae culture system consumes. consume.
本发明输液针头、输气针头和排气针头是一种优选的插拔式快速连接方式，与其他固定式接头方式有等效技术功效。The infusion needle, the air delivery needle and the exhaust needle of the present invention are a preferred plug-in quick connection method, and have equivalent technical effects with other fixed connection methods.
本发明所述的吊袋式微藻养殖系统的运行方法如下：The operating method of the hanging bag type microalgae cultivation system of the present invention is as follows:
1）将两端封闭的吊袋式微藻光反应器吊挂在辅助支架上，将系统设备安装好后，通过输液针头、输气针头或排气针头向袋内加装微藻藻种；1) Hang the hanging bag-type microalgae photoreactor with both ends closed on the auxiliary bracket, and after the system equipment is installed, add microalgae to the bag through the infusion needle, gas delivery needle or exhaust needle;
2）将配置好的营养液注入营养罐内并密封罐口，接通输液输气系统，开启气源输出阀，在营养罐内达到一定压力后，开启输液阀和输气阀，营养罐内的液体空间通过输液阀向吊袋式微藻光反应器输送营养液，营养罐内的气体空间通过输气阀向吊袋式微藻光反应器输送富含CO2气体的混合气体，输液输气量通过调节输液阀和输气阀的开关调节；2) Inject the prepared nutrient solution into the nutrition tank and seal the mouth of the tank, connect the infusion and gas delivery system, open the air source output valve, and open the infusion valve and the gas delivery valve after reaching a certain pressure in the nutrition tank. The liquid space in the nutrient tank delivers the nutrient solution to the hanging bag microalgae photoreactor through the infusion valve, and the gas space in the nutrition tank delivers the mixed gas rich in CO2 gas to the hanging bag microalgae photoreactor through the gas delivery valve, and the infusion gas volume By adjusting the switch of the infusion valve and the gas delivery valve;
3）未被微藻利用的气体，一部分以气泡形式储存在吊袋式微藻光反应器的隔断线内，另一部分由排气针头排出，经过气袋和压气机组成的气体回收系统，回到气源继续循环使用；3) Part of the gas that is not used by microalgae is stored in the partition line of the hanging bag microalgae photoreactor in the form of bubbles, and the other part is discharged from the exhaust needle, passes through the gas recovery system composed of air bags and compressors, and returns to the The air source continues to be recycled;
4）待到微藻产量达到养殖收获要求后，通过排料管阀，将微藻及培养液一并排出，留取部分藻种在吊袋式微藻光反应器内继续繁殖，如此反复实现微藻的多批次培养；4) After the production of microalgae meets the harvesting requirements, discharge the microalgae and the culture medium together through the discharge pipe valve, and retain some algae species to continue to reproduce in the hanging bag microalgae photoreactor, so as to realize microalgae repeatedly. Multi-batch cultivation of algae;
5）由排料管阀排出的微藻及培养液，经过过滤器、水箱及输液泵组成的培养液回收系统，滤出微藻后对培养液重新调节营养成分后，送回到营养罐继续循环使用。5) The microalgae and culture solution discharged from the discharge pipe valve pass through the culture solution recovery system composed of filters, water tanks and infusion pumps. After the microalgae are filtered out, the nutrients in the culture solution are readjusted, and then sent back to the nutrition tank to continue. recycle.
本发明所述吊袋式微藻养殖系统，在收获微藻并实现微藻与培养液分离后，培养液可作为农作物肥料和水源，输送到农作物根部，实现微藻与农作物之间资源循环利用。The hanging bag microalgae cultivation system of the present invention, after harvesting microalgae and separating the microalgae from the culture solution, the culture solution can be used as crop fertilizer and water source and transported to the roots of the crops to realize resource recycling between the microalgae and the crops.
本发明气源为富含CO2气体的混合气体，混合气体中CO2气体的含量为0.05%～100%。The gas source of the present invention is a mixed gas rich in CO 2 gas, and the content of the CO 2 gas in the mixed gas is 0.05% to 100%.
与已有技术方案相比，本发明具有以下有益效果：Compared with the prior art solutions, the present invention has the following beneficial effects:
本发明所述的吊袋式微藻光反应器与现有的薄膜挂袋式单胞藻光生物反应器相比，采用隔断线实现光反应器的结构扁平化，反应器透光性能更好，倒V形、倒U形或W形隔断线结构，便于气体在水体滞留，增加了CO2气体在水体中的滞留时间，更有利于微藻培养体系对CO2气体的吸收。Compared with the existing film hanging bag type microalgae photoreactor, the hanging bag type microalgae photoreactor according to the present invention adopts the partition line to realize the flattening of the structure of the photoreactor, and the light transmission performance of the reactor is better. The inverted V-shaped, inverted U-shaped or W-shaped partition line structure facilitates the retention of gas in the water body, increases the residence time of CO 2 gas in the water body, and is more conducive to the absorption of CO 2 gas by the microalgae culture system.
与现有的微藻光养殖系统相比，本发明所述的吊袋式微藻养殖系统结构更简单，制造和使用成本低廉，与目前开放池养殖系统相比，可以实现封闭式清洁高品质微藻养殖要求。吊袋式微藻养殖系统采用高空养殖，可满足立体、大面积、成本低的经济性养殖需求。Compared with the existing microalgae photoculture system, the hanging bag type microalgae culture system of the present invention has a simpler structure and lower manufacturing and use costs. Compared with the current open pond culture system, it can realize closed clean high-quality microalgae. Algal farming requirements. The hanging bag microalgae cultivation system adopts high-altitude cultivation, which can meet the economical cultivation needs of three-dimensional, large area and low cost.
附图说明Description of drawings
图1为本发明的吊袋式微藻养殖系统组成示意图；Fig. 1 is the composition schematic diagram of hanging bag type microalgae cultivation system of the present invention;
图2为本发明的吊袋式微藻光反应器结构示意图。Fig. 2 is a structural schematic diagram of the hanging bag microalgae photoreactor of the present invention.
图中：1-吊袋式微藻光反应器；2-气源；3-营养罐；4-输液管；5-输液阀；6-输液针头；7-输气管；8-输气阀；9-输气针头；10-排气针头；11-排料管阀；12-布气软管；13-透明薄膜袋；14-隔断线；15-辅助支架；16-气袋；17-排气管阀；18-压气机；19-过滤器；20-水箱；21-排液管阀；22-输液泵；23-输液隔断阀。In the figure: 1-hanging bag microalgae photoreactor; 2-air source; 3-nutrition tank; 4-infusion tube; 5-infusion valve; 6-infusion needle; 7-air delivery tube; 8-air delivery valve; -air delivery needle; 10-exhaust needle; 11-discharge pipe valve; 12-air distribution hose; 13-transparent film bag; 14-partition line; 15-auxiliary bracket; 16-air bag; 17-exhaust Pipe valve; 18-compressor; 19-filter; 20-water tank; 21-discharge pipe valve; 22-infusion pump; 23-infusion isolation valve.
下面对本发明进一步详细说明。但下述的实例仅仅是本发明的简易例子，并不代表或限制本发明的权利保护范围，本发明的保护范围以权利要求书为准。The present invention will be further described in detail below. However, the following examples are only simple examples of the present invention, and do not represent or limit the protection scope of the present invention, and the protection scope of the present invention shall be determined by the claims.
具体实施方式Detailed ways
下面结合附图并通过具体实施方式来进一步说明本发明的技术方案。The technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and through specific implementation methods.
为更好地说明本发明，便于理解本发明的技术方案，本发明的典型但非限制性的实施例如下：For better illustrating the present invention, facilitate understanding technical scheme of the present invention, typical but non-limiting embodiment of the present invention is as follows:
一种吊袋式微藻光反应器，所述吊袋式微藻光反应器包括由端部封闭的透明薄膜袋13形成的反应器主体；所述透明薄膜袋13的内部空间由至少一条隔断线14分割为多空间结构，所述隔断线14存在非连续断开段。A hanging bag type microalgae photoreactor, the hanging bag type microalgae photoreactor comprises a reactor main body formed by a transparent film bag 13 closed at the end; Divided into a multi-space structure, the partition line 14 has discontinuous disconnected segments.
所述隔断线14由数组并列排布的倒V形、倒U形或W形的线条组成。The partition line 14 is composed of an array of inverted V-shaped, inverted U-shaped or W-shaped lines arranged side by side.
所述透明薄膜袋13的上部设有营养液入口和排气口，底部设有进气口和排料口。The top of the transparent film bag 13 is provided with a nutrient solution inlet and an exhaust port, and the bottom is provided with an air inlet and a discharge port.
一种吊袋式微藻养殖系统，所述系统中包括至少一个如上所述的吊袋式微藻光反应器1。A hanging bag microalgae culture system, the system includes at least one hanging bag microalgae photoreactor 1 as described above.
所述系统主要由输液输气系统、吊袋式微藻光反应器1、气体回收系统、培养液回收系统和辅助支架15组成。The system is mainly composed of an infusion and gas delivery system, a hanging bag microalgae photoreactor 1 , a gas recovery system, a culture solution recovery system and an auxiliary support 15 .
所述输液输气系统包括营养罐3和连入营养罐3底部的气源2。所述营养罐3分为上部的气体空间和下部的液体空间；气体空间连接有输气管7，其末端通过输气针头9接入透明薄膜袋13底部的进气口，并与透明薄膜袋13底部的布气软管12连接；液体空间连接有输液管4，其末端通过输液针头6接入透明薄膜袋13上部的营养液入口。The infusion and air delivery system includes a nutrition tank 3 and an air source 2 connected to the bottom of the nutrition tank 3 . Described nutrition tank 3 is divided into the gas space of upper part and the liquid space of lower part; Gas space is connected with air delivery pipe 7, and its end is connected to the air inlet at the bottom of transparent film bag 13 through gas delivery needle 9, and with transparent film bag 13 The air distribution hose 12 at the bottom is connected; the liquid space is connected with an infusion tube 4, the end of which is connected to the nutrient solution inlet on the top of the transparent film bag 13 through the infusion needle 6.
所述输气管7上设有输气阀8；所述输气管7上设有加热器和冷却器；The air delivery pipe 7 is provided with an air delivery valve 8; the air delivery pipe 7 is provided with a heater and a cooler;
所述输液管4上设有输液阀5；所述布气软管12开有若干微孔。The infusion tube 4 is provided with an infusion valve 5; the air distribution hose 12 has a number of micropores.
所述气体回收系统包括依次连接的排气针头10、气袋16和压气机18；所述排气针头10与透明薄膜袋13上部的排气口连通，压气机18与气源2连通；所述气袋16和压气机18之间设有排气管阀17。The gas recovery system includes an exhaust needle 10, an air bag 16 and an air compressor 18 connected in sequence; the exhaust needle 10 communicates with the exhaust port on the top of the transparent film bag 13, and the air compressor 18 communicates with the gas source 2; An exhaust pipe valve 17 is arranged between the air bag 16 and the compressor 18 .
所述培养液回收系统包括依次连接的排料管阀11、过滤器19及水箱20，所述排料管阀11与透明薄膜袋13底部的排料口连通，排料口与透明薄膜袋13底部的进气口共用接口；所述水箱20与营养罐3连通，用于培养液的回用输送。在水箱20内液体静压差不能满足培养液回流营养罐3时，在水箱20与营养罐3之间设置输液泵22。The culture fluid recovery system comprises a discharge pipe valve 11, a filter 19 and a water tank 20 connected in sequence, the discharge pipe valve 11 is communicated with the discharge port at the bottom of the transparent film bag 13, and the discharge port is connected with the transparent film bag 13 The air inlets at the bottom share the interface; the water tank 20 communicates with the nutrition tank 3 for recycling and transporting the culture solution. When the static pressure difference of the liquid in the water tank 20 cannot satisfy the requirement for the culture fluid to return to the nutrition tank 3 , an infusion pump 22 is provided between the water tank 20 and the nutrition tank 3 .
所述水箱20与输液泵22之间设有排液管阀21。A drain pipe valve 21 is provided between the water tank 20 and the infusion pump 22 .
所述输液泵22与营养罐3之间设有输液隔断阀23。An infusion isolation valve 23 is provided between the infusion pump 22 and the nutrition tank 3 .
如图1为本发明所述的吊袋式微藻养殖系统组成示意图。包括一组连体吊袋式微藻光反应器1安装在辅助支架15上，气源2、营养罐3、压气机18、输液泵22及其控制柜固定在集成平台上。输液输气系统中的气源2与营养罐3连接，营养罐3分别与输液管4和输气管7连接，输液管4通过输液针头6连接在吊袋式微藻光反应器1的上部，调节输液阀5采用滴定方式向吊袋式微藻光反应器1内输送营养液，输气管7通过输气针头9连接在吊袋式微藻光反应器1的底部，并与布气软管12连接，向吊袋式微藻光反应器1输送气体，并利用布气软管12实现气体的均匀分布。输入吊袋式微藻光反应器1内的气体，在上升过程中一部分滞留在隔断线14的倒V形隔档内，多余的气体溢出向上移动、层层滞留，强化培养液的扰动。溢出培养液的气体，通过气体回收系统储存在气袋16中，由压气机18排入气源2。Figure 1 is a schematic diagram of the composition of the hanging bag microalgae culture system according to the present invention. It includes a group of conjoined hanging bag microalgae photoreactors 1 installed on the auxiliary bracket 15, and the gas source 2, nutrition tank 3, air compressor 18, infusion pump 22 and its control cabinet are fixed on the integrated platform. The gas source 2 in the infusion and gas delivery system is connected to the nutrition tank 3, and the nutrition tank 3 is connected to the infusion tube 4 and the gas delivery tube 7 respectively, and the infusion tube 4 is connected to the upper part of the hanging bag microalgae photoreactor 1 through the infusion needle 6, and adjusted The infusion valve 5 transports the nutrient solution to the hanging bag microalgae photoreactor 1 by titration, and the air delivery pipe 7 is connected to the bottom of the hanging bag microalgae photoreactor 1 through the air delivery needle 9, and is connected to the air distribution hose 12, The gas is delivered to the hanging bag microalgae photoreactor 1, and the gas distribution hose 12 is used to realize the uniform distribution of the gas. A part of the gas input into the hanging bag microalgae photoreactor 1 stays in the inverted V-shaped partition of the partition line 14 during the ascent process, and the excess gas overflows and moves upwards and stays layer by layer to strengthen the disturbance of the culture solution. The gas overflowing the culture solution is stored in the air bag 16 through the gas recovery system, and is discharged into the gas source 2 by the compressor 18 .
根据气体消耗量向营养罐3内鼓入气体，营养罐3内压力升高，营养罐3下部液体空间的营养液通过输液管4、输液阀5、输液针头6输送到吊袋式微藻光反应器1内，输液流量通过输液阀5调节；同样的，营养罐3上部气体空间的气体通过输气管7、输气阀8、输气针头9输送到吊袋式微藻光反应器1内，由布气软管12将输入吊袋式微藻光反应器1的气体均匀扩散到吊袋式微藻光反应器1的底部区域，输气流量通过输气阀8调节。在吊袋式微藻光反应器1的微藻养殖量达到收获要求时，关闭输气阀8开启排料管阀11，吊袋式微藻光反应器1内微藻与培养液通过与进气口共用的排料口排出，实现吊袋式微藻光反应器1内微藻的排出过滤收集与回收利用。Gas is blown into the nutrition tank 3 according to the gas consumption, the pressure in the nutrition tank 3 rises, and the nutrient solution in the liquid space at the lower part of the nutrition tank 3 is transported to the hanging bag microalgae photoreaction via the infusion tube 4, the infusion valve 5, and the infusion needle 6. In the device 1, the infusion flow rate is regulated by the infusion valve 5; similarly, the gas in the upper gas space of the nutrition tank 3 is transported to the hanging bag type microalgae photoreactor 1 through the air pipe 7, the air valve 8, and the air needle 9, and is controlled by the cloth The gas hose 12 evenly diffuses the gas input into the hanging bag microalgae photoreactor 1 to the bottom area of the hanging bag microalgae photoreactor 1 , and the gas delivery flow is regulated by the gas delivery valve 8 . When the amount of microalgae cultivated in the hanging bag microalgae photoreactor 1 reaches the harvesting requirement, close the gas delivery valve 8 and open the discharge pipe valve 11, and the microalgae and the culture solution in the hanging bag microalgae photoreactor 1 pass through the air inlet Discharging from the shared discharge port realizes the discharge, filtration, collection and recycling of the microalgae in the hanging bag microalgae photoreactor 1 .
图1示出了两个吊袋式微藻光反应器1对称的安装在辅助支架15上，通过并联多支路输液输气系统，实现多个吊袋式微藻光反应器1的联合养殖。Figure 1 shows that two hanging bag-type microalgae photoreactors 1 are symmetrically installed on the auxiliary support 15, and the combined cultivation of multiple hanging bag-type microalgae photoreactors 1 is realized by connecting a multi-branch infusion and gas delivery system in parallel.
图2为吊袋式微藻光反应器1的结构示意图，图中两只吊袋式微藻光反应器1联接在一起，跨装在辅助支架15上，为突出吊袋式微藻光反应器1结构，用于支撑吊袋式微藻光反应器1的辅助支架15在图中被隐去。吊袋式微藻光反应器1由透明薄膜袋13制造，底部被压实封闭形成封闭空间，在吊袋式微藻光反应器1的底部安装有布气软管12，输气针头9穿过透明薄膜袋13插入布气软管12，输液针头6插入透明薄膜袋13，采用滴定模式缓慢加入营养液，排气针头10插入透明薄膜袋13，作为封闭吊袋式微藻光反应器1的排气口。在透明薄膜袋13内，以一定距离间隔压实成倒V形或倒U型或W形隔断线14，隔断线14存在非连续断开段，便于气流和培养物穿过间隔隔断14，也便于改变气体在吊袋式微藻光反应器的流动状态和滞留时间，达到均匀传质效果。Fig. 2 is a structural schematic diagram of the hanging bag type microalgae photoreactor 1, in which two hanging bag type microalgae photoreactors 1 are connected together and straddled on the auxiliary support 15 to highlight the structure of the hanging bag type microalgae photoreactor 1 , the auxiliary support 15 for supporting the hanging bag microalgae photoreactor 1 is hidden in the figure. The hanging bag microalgae photoreactor 1 is made of a transparent film bag 13, and the bottom is compacted and sealed to form a closed space. An air distribution hose 12 is installed at the bottom of the hanging bag microalgae photoreactor 1, and the air delivery needle 9 passes through the transparent film bag. The film bag 13 is inserted into the air distribution hose 12, the infusion needle 6 is inserted into the transparent film bag 13, and the nutrient solution is slowly added in the titration mode, and the exhaust needle 10 is inserted into the transparent film bag 13, which is used as the exhaust of the closed hanging bag microalgae photoreactor 1 mouth. In the transparent film bag 13, an inverted V-shaped or inverted U-shaped or W-shaped partition line 14 is compacted at a certain interval, and there are discontinuous disconnection sections in the partition line 14, which is convenient for air flow and culture to pass through the partition partition 14, and also It is convenient to change the flow state and residence time of the gas in the hanging bag microalgae photoreactor to achieve a uniform mass transfer effect.
具体实施例：Specific examples:
以小球藻培养为例，利用针管取小球藻种源，利用输液针头6将待培养藻种注入吊袋式微藻光反应器1，连接输液与输气管道，以荧光灯为光源进行24小时连续培养，将细胞表面光强调整为50μmol m-2s-1，环境温度为30℃，加注营养液至一定量后，采用滴定方式缓慢加入营养液，每半小时通过布气软管12连续向吊袋式微藻光反应器1内通入CO2与空气混合气体5分钟。随着培养物生长，藻种的不断繁殖数量增多，输入液体增多，输气时间加长，输液输气量根据藻种对营养物质的需求不断增加，试验结果表明，微藻的生长速率达25gm-2d-1，养殖结束后，利用培养液回收系统将培养物过滤收集干燥，实现产品培养与收获。Taking the cultivation of chlorella as an example, the source of chlorella is obtained by using a needle tube, and the algae to be cultivated is injected into the hanging bag microalgae photoreactor 1 by using the infusion needle 6, and the infusion and gas pipeline are connected, and the fluorescent lamp is used as the light source for 24 hours. For continuous culture, the light intensity on the cell surface was adjusted to 50 μmol m -2 s -1 , and the ambient temperature was 30°C. After adding the nutrient solution to a certain amount, the nutrient solution was slowly added by titration, and passed through the air distribution hose 12 every half hour. Continuously feed the mixed gas of CO2 and air into the hanging bag microalgae photoreactor 1 for 5 minutes. As the culture grows, the number of algae species will increase continuously, the input liquid will increase, and the time of gas transmission will be prolonged . 2 d -1 , after the cultivation is over, use the culture fluid recovery system to collect and dry the culture by filtration to realize product cultivation and harvesting.
申请人声明，本发明通过上述实施例来说明本发明的详细结构特征以及方法，但本发明并不局限于上述详细结构特征以及方法，即不意味着本发明必须依赖上述详细结构特征以及方法才能实施。所属技术领域的技术人员应该明了，对本发明的任何改进，对本发明所选用部件的等效替换以及辅助部件的增加、具体方式的选择等，均落在本发明的保护范围和公开范围之内。The applicant declares that the present invention illustrates the detailed structural features and methods of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned detailed structural features and methods, that is, it does not mean that the present invention must rely on the above-mentioned detailed structural features and methods. implement. Those skilled in the art should understand that any improvement to the present invention, the equivalent replacement of selected components in the present invention, the addition of auxiliary components, the selection of specific methods, etc., all fall within the scope of protection and disclosure of the present invention.
以上详细描述了本发明的优选实施方式，但是，本发明并不限于上述实施方式中的具体细节，在本发明的技术构思范围内，可以对本发明的技术方案进行多种简单变型，这些简单变型均属于本发明的保护范围。The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solutions of the present invention. These simple modifications All belong to the protection scope of the present invention.
另外需要说明的是，在上述具体实施方式中所描述的各个具体技术特征，在不矛盾的情况下，可以通过任何合适的方式进行组合，为了避免不必要的重复，本发明对各种可能的组合方式不再另行说明。In addition, it should be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable way if there is no contradiction. The combination method will not be described separately.
此外，本发明的各种不同的实施方式之间也可以进行任意组合，只要其不违背本发明的思想，其同样应当视为本发明所公开的内容。In addition, various combinations of different embodiments of the present invention can also be combined arbitrarily, as long as they do not violate the idea of the present invention, they should also be regarded as the disclosed content of the present invention.
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