CN104857315B

CN104857315B - Particle for strengthen immunity

Info

Publication number: CN104857315B
Application number: CN201510226240.8A
Authority: CN
Inventors: 杨槐; 梁斌; 张丽艳; 唐靖雯; 潘梅; 王传方
Original assignee: GUIZHOU WARMEN PHARMACEUTICAL CO Ltd
Current assignee: GUIZHOU WARMEN PHARMACEUTICAL CO Ltd
Priority date: 2015-05-06
Filing date: 2015-05-06
Publication date: 2018-04-13
Anticipated expiration: 2035-05-06
Also published as: CN104857315A

Abstract

The present invention relates to the particle and preparation method for strengthen immunity.Specifically, the present invention relates to a kind of particle for strengthen immunity, it is made of dendrobium candidum and peacilomyce hepiahi bacterium powder are extracted, and wherein the weight ratio of dendrobium candidum and peacilomyce hepiahi bacterium powder is 100：100~200, wherein both dendrobium candidum and peacilomyce hepiahi bacterium powder be reinstate water extraction and the concentrated dry extract being dried to obtain be added in the granule.The present invention using specified quantitative dendrobium candidum and peacilomyce hepiahi bacterium powder it is extracted made of granule there is unexpected advantageous feature, such as seek peace physicochemical property with excellent biological characteristics.

Description

Particle for strengthen immunity

Technical field

The invention belongs to life science, is related to a kind of particle for strengthen immunity.More particularly to one kind includes The product of dendrobium candidum, it is more special more particularly to one kind granule as made from dendrobium candidum and peacilomyce hepiahi bacterium powder It is not to be related to one kind by the extracted rear obtained granule of dendrobium candidum and peacilomyce hepiahi bacterium powder.The invention further relates to this The preparation method of kind granule.The granule of the present invention has the advantages that existing dendrobium candidum product is incomparable, particularly exists The advantages of in terms of strengthen immunity.

Background technology

Immune is a kind of biological answer-reply process of body " identification " and " exclusion " non-antigen, to maintain internal physiology Balance.Immunity function is the basis of health, immune dysfunction and it is low when, the energy of body fight infections can be reduced Power, reduces identification and removes the histocyte ability of itself aging, reduces killing and removes the energy of abnormal sudden change cell in vivo Power, causes the decline of human body premunition and vis medicatrix naturae.And modern society, the accelerating rhythm of life, the increase of competitive pressure, make to be permitted More people are in nervous working and learning state, and body, which is chronically at this tense situation, will suppress immune function；Meanwhile tightly Working and learning allow more people to have to convenient fast food, the unbalance of endotrophic element is caused, if body is long-term In a state in which immunity of organisms can be declined, the sub-health state of body is ultimately resulted in.

According to《China Youth News-youth Shi Xun》Report, the Ministry of Public Health once investigated 10 city working clans, find People in sub-health state accounts for 48%, and wherein coastal cities are higher than inland city, and brain worker is higher than physical labourer, in Year, people was higher than young people.Meanwhile " Chinese health care science and technology association " is good for national 16 provinces, cities before affiliated institutions of the Ministry of Public Health Health status investigation, and announce in July, 2002 the result shows that, the ratio that Pekinese is in " inferior health " state is 75.3%, on Sea is that 73.49%, Guangdong is 73.41%.If human body is chronically at sub-health state, body and intellectual development can be caused not It is good, also easily induce major disease.

As sub-health population gradually increases, many consumers wish to improve physical condition by edible health food, The city above county level of inland of China 654, the adult city of 20 years old~64 years old are occupied according to Shanghai Zhi Lian market surveys Co., Ltd The sample investigation of the people shows that what consumer's purchase health food purpose to be reached took the first four place position is respectively：Adjust immune Power, improve bone condition, be antifatigue, improving sleep.Wherein southeastern coast, Little Valleys In The Middle Reaches of The Yellow and the consumer of southwest pay attention to adjusting The relatively other areas of ratio for saving immunocompetence are higher.It can be seen from the above that " advocating health care, put prevention first " viewpoint is extensive by the masses Receive, strengthen immunity, improve the disease resistance of body also as common recognition.

It follows that society is changing in progress, the life style of people, rhythm of life is accelerating, and research and development have The health food of strengthen immunity function has the huge market demand and vast potential for future development.

Dendrobium candidum (scientific name：Dendrobium officinale Kimura et Migo), also known as：Ribbed hedyotis herb, Yunnan Iron sheet, iron sheet bucket maple.Belong to micro- specific item, the perennial herbaceous plant that grows nonparasitically upon another plant of orchid family.Stem is upright, cylinder, 9-35 centimetres long, thick 2-4 Millimeter, sepal and petal yellow green, near similar, long round shape lanceolar, is about 1.8cm, wide 4~5mm, 3~June of florescence.Mainly It is distributed in the ground such as Chinese Anhui, Zhejiang, Fujian.Its stem is used as medicine, and belongs to the yin tonics in tonic.

Dendrobium candidum is herbaceos perennial, is distributed widely in the ground such as Guangxi, Yunnan, Zhejiang, Fujian, is typically distributed about On the dark and damp rock in mountainous region half.

The morphological feature of dendrobium candidum：Stem is upright, cylinder, 9-35 centimetres long, 2-4 millimeters thick, not branch, has more piece, 1-3-1.7 centimetres of internode length, often in middle part 3-5 pieces of leaf of above alternate；Ye Erlie, papery, long round shape lanceolar, long 3-4 (- 7) Centimetre, wide 9-11 (- 15) millimeter, apex is blunt and how many hook turn, and base portion is downward to embrace the sheath of stem, and edge and middle rib are often with pale purple Color；Leaf sheath often with purple plague purpura, when old its upper limb and stem pine from and opens, and leave 1 ashen gap of ring-type with saving.

Raceme is often sent from the old stem top for having fallen leaf, has 2-3 flower；Common peduncle is 5-10 millimeters long, base portion tool 2-3 Piece short sheath；Rachis inflection sigmoid, it is 2-4 centimetres long；Petal piece dry film matter, shallow white is avette, 5-7 millimeters long, and apex is slightly It is blunt；Bennet and ovary are 2-2.5 centimetres long；Sepal and petal yellow green, nearly similar, long round shape lanceolar is about 1.8 centimetres, wide 4-5 millimeters, the sharp point of apex, has 5 arteries and veins；Side sepal base portion is broader, about 1 centimetre wide；Calyx capsule cone, is about 5 millimeters, end It is circular；Lip white, base portion have the corpus callosum of 1 green or yellow, and ovum shape lanceolar is more slightly shorter than sepal, middle part reflexed, apex Anxious point, does not split or unobvious 3 is split, the tool aubergine striped of both sides below middle part, and how much wavy edge is；Lip disk gathers thin papillary Hair, and have 1 aubergine patch more than middle part；Stamen column yellow green, is about 3 millimeters, apex both sides respectively have 1 purple point；Stamen Column foot yellow green band aubergine striped, thinly covered hair；Anther cap white, long ovate triangle, is about 2.3 millimeters, the nearly sharp point in top is simultaneously And 2 split.The month at florescence 3-6.

In terms of habit, dendrobium candidum is suitably in nice and cool, moistening, the ambient growth of clear air.Usually it is born in sea Pull out up on the dark and damp rock in 1600 meters of mountainous region half, the environment of happiness warm and moist weather and half cloudy half light, can not resist cold.

In terms of geographical distribution, dendrobium candidum is distributed widely in the Anhui west and south (Dabie Mountain), Eastern Part of Zhejiang Province, China (Yinxian County, day Platform, fairy house), In Western Fujian Province (Ninghua), the Guangxi northwestward (Tiane), Sichuan, southeastern Yunnan (stone screen, mountain of papers, Malipo, west Farmland).

In terms of propagation method, fast numerous method and plant division cutting propagation etc. in a organized way.

Tissue rapid propagation method：The hardening of 14~21 days should be first carried out before tissue-cultured seedling cultivation, concrete measure is to move to tissue-cultured seedling Hardening room, makes its gradual reform of nature environment in the environment of open change, can bottle outlet plantation when tissue-cultured seedling leaf color is dark green.

First bottle cap is opened before bottle outlet, allows bottle seedling to be placed in outdoor air 2~3 days, allows its reform of nature humiture.So After carry out washing seedling, the seedling with culture medium is gently taken out when washing seedling, is placed in basin and cleans, pollutes seedling and bare-root seeding or few root Seedling should be separated.Tissue-cultured seedling is first cleaned with tap water, should mainly wash agar off, in case agar is mouldy to cause rotten, then Rinsed with clear water again.Size and quality when washing seedling preferably based on seedling are synchronously classified, and to cultivate, to manage, are improved The survival rate of tissue-cultured seedling simultaneously makes its growth neat.

Plant division cutting propagation method：Selected during plant division life in 1 year or life in 2 years, color and luster it is light green, it is healthy and strong, sprout more, well developed root system and The plant of no disease and pests harm makees kind of a strain, cuts off deadwood, disconnected branch, old branch and long fibrous root, strain clump is cut, and is divided into little Cong, every clump Stem strain with leaf 5~7, you can plantation.

In terms of cultivation technique, the planting time of dendrobium candidum is usually annual spring and autumn, and spring is better than autumn. In Zhejiang area, the Best Times of officinal dendrobium stem plantation are annual mid-April to late June, and this period, temperature was 12~25 DEG C, and air humidity is larger, the test tube seedling transplanting survival rate after bottle outlet is higher and growth time is longer；Secondly mid-September is extremely Late October, the transplanting of this period are important to carry out the antifreeze work of cold-resistant.The cultivation matrix of dendrobium candidum is High efficiency and high quality cultivation Key, the biological nature of dendrobium candidum require the existing good water-retaining property of cultivation matrix to have ventilating air permeability, large-scale production again It is required that cultivation matrix raw material is easy to get, is easy to operate.Report mesostroma has sphagna, rubble, peanut shell, moss, coconut skin, pine tree Skin, sawdust, charcoal, wooden unit etc., application in production is mainly that bark, sawdust, or bark, sawdust, rubble, organic fertilizer mix Thing, wherein bark are ground into 2~below 3cm granules.Officinal dendrobium stem plantation requirement carries out in greenhouse, the construction requirement of greenhouse Accomplish to divulge information, shade and keep off the rain, have a fly net, and according to the habit of dendrobium candidum, consider the illumination in place, temperature, humidity, The natural causes such as ventilation.The preference temperature of Growth of Dendrobium candidum is 15~30 DEG C, and in Zhejiang area, summer is more hot, generally adopts Covered with 80% sunshade net, raise plastic film, cooled down with profit；Winter is colder, is used under 30%~50% sunshade degree Double-plastic closing insulation.Dendrobium candidum requires to keep matrix moistening, and air humidity keeps more than 80% preferably, but not Can ponding, it is best using sprinkling irrigation or trickle irrigation during watering, must not irrigation.Different seasons it is different regional irrigation amount it is also different.

Peacilomyce hepiahi bacterium powder, that is, cordyceps, is to use deep cooling superfine communication technique, is divided from natural cordyceps A kind of Mycophyta active material separated out, forms through liquid deep layer fermenting culture.Peacilomyce hepiahi bacterium powder is country 65, seven Five emphasis key scientific and technological projects achievements, and be in a kind of new Chinese medicine (raw material) authentication code of 1989 Nian Huo Ministry of Public Health approval [(89) defend the quasi- word of medicine Z-17], this is also that health ministry carries out first Chinese medicine one produced since " provisions for new drugs approval " Kind new medicine.Peacilomyce hepiahi bacterium powder system Cordyceps Militaris separating obtained from fresh cordyceps sinensis --- Paecilomyces hepiali chen Cs-4 Bacterial strain, is made through Submerged fermentation, processing.

Among vicennial clinical practice, its good clinical effectiveness and reliable security obtain domestic and international medicine deeply The accreditation of experts and scholars.And with being sent the documents by health ministry on March 23rd, 2001：Method prison (2001) No. 84 texts of hair are defended, are confirmed as Fungi list available for health food.

Gong Xiaojian etc. is using Carbon grain Kuo clear law, dinitrofluorobenzene inducing mouse delayed allergy test method(s) and hemolysin Test method(s), observes artificial Cordyceps sinensis polysaccharide to non-specific and specific immune function influence.The result shows that artificial aweto intracellular And exocellular polysaccharide can significantly increase phagocyte imdex K and phagocytic index α, strengthen the phagocytic function of mononuclear macrophage；Born of the same parents Interior polysaccharide can significantly increase mice ear degree, and the mouse delayed allergy of enhancing dinitrofluorobenzene induction, is improved molten Sanguinin is horizontal, prompts artificial aweto intracellular polyse to have the function that to strengthen cellular immunity and humoral immunity.

Zhong Jianchun etc. is using the weight method of mouse, spleen, thymic factor D injection method, delayed allergy test method(s), and list Core macrophage phagocytic function test method, which have studied polyoses extract, influences mouse immune.Test result indicates that:Artificial aweto Polyoses extract can increase mouse thymus quality；Improve the mouse delayed allergy that 2,4- dinitrofluorobenzene induces； The phagocytic activity of mononuclear macrophage can be significantly improved, but mouse weight is not influenced.Therefore Fermented Cordyceps polysaccharide Have the function that to strengthen immune function of mice.

Guo Hongchun etc. elaborates that cordyceps sinensis can significantly improve t helper cell and Lyt-1/Lyt-2 (t helper cells/T suppressions Cell processed) in the ratio of peripheral blood and spleen, while spleen weight, macrophage quantity and macrophage activity can be improved.

The comparison of peacilomyce hepiahi bacterium powder and natural cordyceps, peacilomyce hepiahi bacterium powder are that natural cordyceps exist Upgrading substitute in pharmacy industry.High-quality natural cordyceps control is produced with Qinghai, carries out component comparative analysis the results show that bat Bat moth Paecilomyces varioti bacterium powder and natural cordyceps chemical composition are basically identical, pharmacological action also with the basic phase of natural cordyceps Together.Natural cordyceps price too expensive, is not suitable for ordinary people's long-term use, and cordyceps sinensis on the market is very much All adulterate, the product mixed the spurious with the genuine is more, and ordinary people is not easy to tell truth from falsehood；And wild cordyceps sinensis is due to life Long environment can not be controlled strictly, the problems such as being relatively also easy to produce heavy metals exceeding standard, pollute.And peacilomyce hepiahi bacterium powder is as natural worm The upgrading substitute of grass, preferably solves the above problem, realizes the great target for allowing cordyceps sinensis to enter into common people house.

Peacilomyce hepiahi bacterium powder, which uses to produce from Yushu district, Qinghai area, isolates and purifies gained ergot in fresh cordyceps sinensis Section's fungi peacilomyce hepiahi bacterium strain (CS-4 bacterial strains), forms through mycelium drying obtained by bioengineering liquid deep layer fermenting.

The pharmacological property of peacilomyce hepiahi bacterium powder：It is sweet in flavor, it is warm-natured.Return lung, kidney channel.Pharmacology：Immune function, makes macrophage Phagocytic percentage and phagocytic index substantially increase.Major function：Invigorating the lung and the kidney, hemostasis and phlegm, cures mainly chronic cough and dyspnea of deficiency type, deficiency of the kidney sun The diseases such as impotence, seminal emission, soreness of waist and knee joint.Its core functional component includes：Polysaccharide：More than or equal to 8.0%, start macrophage, enhancing Immune and hematopoietic potential；Adenosine：More than or equal to 0.3%, pressure of releiving and intense strain, improve sleep, and enhancing is physical, endurance, It is antifatigue.Man is not easy tired；Ms can invigorate blood circulation, and lift qi and blood, and antenatal postpartum recuperating more preferably, is had rosy cheeks, and hand warms up sufficient temperature, Beauty is healthy and strong.And it can effectively maintain healthy cholesterol；Cordycepic acid (PEARLITOL 25C)：More than or equal to 14.0%, happy organ, cough-relieving Relieving asthma, reduce respiratory problems, strengthen adrenaline；Ergosterol：More than or equal to 0.65%, serum cholesterol and sweet can be reduced Oily three ester contents, strengthen heart and cardiovascular function, suppress harmful cellular growths；Sudismase (SOD)：It is on a small quantity, anti-oxidant, Strengthen brain oxygen supply amount, lift mental and memory, pressure of releiving.Body or function aging can be resisted, makes the heart, lung, kidney and brain Keep fit in portion；The oxygen radical that the increase of SOD contents is produced for removing organism metabolism, or internal some medicines are entered by the external world The free radical and can be played a positive role by the free radical that radiation produces that thing or toxicant metabolism produce；Protein：31.0%- 35.0%, it is to form human body cell and the basic substance of vital movement.

Peacilomyce hepiahi bacterium powder target user is extremely wide, including：Man：Invigorating kidney yang, moistening lung, improves sleep quality, relaxes Slow stress, supplements energy, not easy to fatigue, respiratory tract anaphylaxis symptom of releiving；Female：Nourishing qi and blood, solves menses worry, brothers It is ice-cold to wait disease；To anti-aging, make skin children tender, white touched with red；And build up resistance, improve constitution, stress of releiving；Always： Enriching yin and nourishing kidney are empty, strengthen cardio-pulmonary function, supplement energy, promote spleen function, and strengthening the bones and muscles, strengthens blood flow, strengthens eyesight； Children：Resistance building, respiratory problems of releiving, reduce the symptoms such as flu, cough, nose sensitivity.Resist learning fatigue, lifting note Recall power, brain tonic and intelligence development.

Although there is all products for being chiefly used in strengthen immunity particularly health food in the prior art.But with excellent The product such as health food of different strengthen immunity performance is still desirable.

The content of the invention

Present invention aims at provide a kind of product for strengthen immunity particularly health food, and expecting this Health food has excellent biological effectiveness.The present inventor is surprisingly, it was found that dendrobium candidum and bat using specified quantitative Granule made of bat moth Paecilomyces varioti bacterium powder is extracted has unexpected advantageous feature, therefore the present invention is accomplished. A kind of particle for strengthen immunity

Therefore, first aspect present invention provides a kind of particle for strengthen immunity, it can also claim in the present invention For dendrobium candidum particle (it is also referred to as granule), it is by dendrobium candidum and peacilomyce hepiahi bacterium powder is extracted is made 's.

The particle for strengthen immunity described in any embodiment, wherein dendrobium candidum according to a first aspect of the present invention Weight ratio with peacilomyce hepiahi bacterium powder is 100：100~200, such as weight ratio is 100：120~180, such as weight ratio For 100：150.It has been unexpectedly discovered that with aforementioned proportion, particularly using weight ratio as 100：Particle made from 150 ratios More excellent biological activity is presented in granule made from other ratios than it for agent.

The particle for strengthen immunity described in any embodiment, wherein dendrobium candidum according to a first aspect of the present invention With both peacilomyce hepiahi bacterium powder be reinstate water extraction and the concentrated dry extract being dried to obtain be added to the particle In agent.

The particle for strengthen immunity described in any embodiment according to a first aspect of the present invention, wherein the dry leaching Cream powder is prepared according to following method：Weigh dendrobium candidum, peacilomyce hepiahi bacterium powder, extracting in water, extracting solution decompression Concentration, dry, pulverize, obtain dry extract.It is traditional extraction work well known in the art to carry out said extracted as Extraction solvent using water Skill.Such as the dosage of water is 5-30 times of medicinal material gross weight.Such as can extract 1-5 times, when each 1-3 is small.Such as merging Filtrate can obtain medicinal extract through being concentrated under reduced pressure.Such as gained medicinal extract can be vacuum dried so as to obtain dry extract.

The particle for strengthen immunity described in any embodiment according to a first aspect of the present invention, wherein the dry leaching Cream powder is prepared according to following method：Dendrobium candidum, peacilomyce hepiahi bacterium powder are weighed, adds 20 times of amount water, extracts 2 times, Every time 1 it is small when, merge extracting solution.Extracting solution is concentrated under reduced pressure (70-80 DEG C of temperature, 0.06~0.08Mpa of vacuum) to relatively close Spend for 1.20-1.25 (60 DEG C), obtain medicinal extract, medicinal extract vacuum drying (60~70 DEG C of temperature, 0.06~0.08Mpa of vacuum), powder It is broken, obtain dry extract.

The particle for strengthen immunity described in any embodiment according to a first aspect of the present invention, wherein also adding medicine Use auxiliary material.

The particle for strengthen immunity described in any embodiment according to a first aspect of the present invention, wherein described medicinal Auxiliary material is to be selected from following carbohydrate：Sucrose, lactose, glucose, fructose, mannitol etc..

The particle for strengthen immunity described in any embodiment, wherein dendrobium candidum according to a first aspect of the present invention The weight ratio of medicinal material and institute's adding carbohydrate is 100：200~300, such as weight ratio is 100：220~280, such as weight ratio is 100：230~270, such as weight ratio is 100：260.It has been unexpectedly discovered that use particular kind of carbohydrate, Ke Yiyou Help obtain significantly more stable granule.In view of carbohydrate used is only the filler of this area routine, but it is in this hair The bright middle effect for producing improvement stability is that the prior art is completely unexpected.

The particle for strengthen immunity described in any embodiment according to a first aspect of the present invention, it, which has, is less than 8% moisture.

The particle for strengthen immunity described in any embodiment according to a first aspect of the present invention, it, which has, is less than 6% moisture.

The particle for strengthen immunity described in any embodiment according to a first aspect of the present invention, it, which has, is less than 5% moisture.

The particle for strengthen immunity described in any embodiment according to a first aspect of the present invention, it substantially shines What following steps were prepared：

(1) dry extract is prepared：Dendrobium candidum, peacilomyce hepiahi bacterium powder are weighed, extracting in water, merges extracting solution；

(2) extracting solution is concentrated under reduced pressure, and obtains medicinal extract, medicinal extract vacuum drying, crushes, obtain dry extract；

(3) weigh pharmaceutic adjuvant and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, the wet system of softwood Grain, it is dry, to obtain the final product.

(1) dry extract is prepared：Dendrobium candidum, peacilomyce hepiahi bacterium powder are weighed, adds 20 times of amount water, extracts 2 times, every time 1 Hour, merge extracting solution；

(2) extracting solution be concentrated under reduced pressure (70-80 DEG C of temperature, 0.06~0.08Mpa of vacuum) to relative density be 1.20- 1.25 (60 DEG C), obtain medicinal extract, medicinal extract vacuum drying (60~70 DEG C of temperature, 0.06~0.08Mpa of vacuum), crushes, obtain dry leaching Cream powder；

Further, second aspect of the present invention provides a kind of method for preparing the particle for strengthen immunity, the party Method comprises the following steps：

Method described in any embodiment according to a second aspect of the present invention, it comprises the following steps：

Method described in any embodiment according to a second aspect of the present invention, wherein the obtained strengthen immunity that is used for Particle has the moisture less than 8%.

Method described in any embodiment according to a second aspect of the present invention, wherein the obtained strengthen immunity that is used for Particle has the moisture less than 6%.

Method described in any embodiment according to a second aspect of the present invention, wherein the obtained strengthen immunity that is used for Particle has the moisture less than 5%.

Method described in any embodiment, wherein dendrobium candidum and peacilomyce hepiahi bacterium according to a second aspect of the present invention The weight ratio of powder is 100：100~200, such as weight ratio is 100：120~180, such as weight ratio is 100：150.

Method described in any embodiment according to a second aspect of the present invention, wherein the pharmaceutic adjuvant is selected from following Carbohydrate：Sucrose, lactose, glucose, fructose, mannitol etc..

Method described in any embodiment according to a second aspect of the present invention, wherein dendrobium candidum medicinal material and institute's adding carbohydrate Weight ratio be 100：200~300, such as weight ratio is 100：220~280, such as weight ratio is 100：230~270, such as Weight ratio is 100：260.

In the step of above-mentioned preparation method of the invention, although the specific steps of its description are in some details or language The step of described in preparation example in description with following detailed description part, is otherwise varied, however, people in the art The detailed disclosure of member's full text according to the present invention can summarize approach described above step completely.

Any embodiment of the either side of the present invention, can be combined with other embodiments, as long as they are not Contradiction occurs.In addition, in any embodiment of either side of the present invention, any technical characteristic can be adapted for other realities The technical characteristic in scheme is applied, as long as they are not in contradiction.

The invention will be further described below.

All documents recited in the present invention, their full content are incorporated herein by reference, and if these are literary When offering expressed implication and the inconsistent present invention, the statement of the present invention is subject to.In addition, the various terms that use of the present invention and Phrase has well known to a person skilled in the art general sense, nonetheless, the present invention remain desirable at this to these terms and Phrase is described in more detail and explains, the term and phrase referred to is if any inconsistent with common art-recognized meanings, with institute's table of the present invention Subject to the implication stated.

It is used for the particle of strengthen immunity according to the present invention, which uses unit dose package Form is packed.For example, the amount in each unit dose package for the particle of strengthen immunity is converted to the gauge of iron sheet lime dry measure used in former times It can be such as, but not limited to about 0.5g, about 1g, about 1.5g, about 2g, about 2.5g, about 3g, about 5g etc..

Qin Han dynasty《Sheng Nong's herbal classic》Record, dendrobium candidum " in main wound, eliminating impediment, lower gas, tonifying five zang organs consumptive disease it is thin it is thin, Qiang Yin, long term usage thickness stomach "；It is classical into Taoist school medicine of the book before more than 1,000 years《Taoist Scriptures》Dendrobium candidum is classified as to " China nine is big First of celestial grass "；Li Shizhen (1518-1593 A.D.) exists《Compendium of Materia Medica》Middle evaluation dendrobium candidum " strong the moon strengthening the essence, thick stomach, mend in never enough, flat stomach gas, Long muscle, intelligence development is except frightened, macrobiosis of making light of one's life by commiting suicide "；The stem of the dendrobium candidum among the people for being called " help celestial grass " modern times can clearing heat and promoting fluid, Anti-inflammatory analgetic, clear and rich throat, has the effect of fine to treatment hoarse voice.The stem of noble dendrobium has good antifatigue, the work of resist oxygen lack With.Dendrobium candidum inside pharmacy, category is numerous, cheap, is often served as with water plant, and the country only has a few big product Board can buy real dendrobium candidum, and using good fortune, dendrobium candidum and the two old brands of Tongrentang are the most authentic as representative near the house. Tongrentang's price is most expensive, and packaging is more beautiful, is suitable for giving a present.

The effect of being used for stomach-Yin deficiency and consumption of body fluid caused by febrile disease card on dendrobium candidum.This product is longer than nourishing the stomach-yin, promotes the production of body fluid to quench thirst, simultaneous Can clearing away the stomach-heat.Consumption of body fluid caused by febrile disease is cured mainly, the black card of the dry tongue of polydipsia, tongue, is often used together, such as with product such as root of Chinese trichosanthes, the fresh dried rhizome of rehmannia, ophiopogons《When Disease opinion》Heat-clearing Bao Jinfa.The gastral cavity pain, swelling and aching of gum, aphthae for controlling the stomach energy deficiency of Yin can be with the product such as the dried rhizome of rehmannia, ophiopogon, radix scutellariae With use.

The effect of being used for syndrome of deficiency of kidney yin on dendrobium candidum.This product again can nourishing kidney-yin, and the fire of deficiency type can be dropped, be lost suitable for kidney yin The dark unknown, muscles and bones impotence of the mesh of void and fire excess from yin deficiency, the card such as hectic fever due to yin labor heat.Kidney deficiency, mesh secretly unknown person, often with the fruit of Chinese wolfberry, The product such as prepared rhizome of rehmannia, Semen Cuscutae with use, as Shiyu Yeguang Wan (《Former machine opens micro-》).Kidney deficiency, muscles and bones impotence person, often with cultivated land, The filling liver kidney such as Fructus Corni, the bark of eucommia, the root of bidentate achyranthes, the product of strengthening the bones and muscles are the same as use.Kidney deficiency hyperactivity of fire, the hot person of hectic fever due to yin labor, preferably with radix rehmanniae recen, matrimony vine The nourishing kidney-yins such as son, Cortex Phellodendri, Radix picrorrhizae, the product of reducing the asthenic fever are the same as use.

Dendrobium candidum is sweet in flavor, cold nature.Energy replenishing the vital essence and removing heat, reinforcing stomach reg fluid.Dendrobium candidum includes the alkaloids such as dendrobine, glues Liquid matter, starch, polysaccharide, mannose etc..Dendrobium candidum has certain antipyretic effect, can promote gastric secretion, is aid digestion, having increasing Excitometabolic, the anti-ageing effect of waiting for a long time.Clinically dendrobium candidum can be used for heat consuming body fluid, low-heat polydipsia, the red few tongue of tongue；Stomach-Yin is not Foot, thirsty and dry pharynx, inverse few food of vomitting, vague epigastralgia, tongue light lack tongue；The deficiency of the kidney yin, blurring of vision.

People have many dendrobium candidum eating methods.For example, enter tea：20~30 grams of fresh dendrobium candidum is taken, cleans and goes Clothing shave or segment, which are clapped, to be split, and with jujube several or a little matrimony vine, adds clear water some (according to oneself amount of drinking water), with intense fire by water Boil, then it is i.e. drinkable to be cooked by slow fire more than 30 minutes.Or direct boiling water Instant Drinks.Food is finished, and dendrobium candidum is taken out edible.Enter Meals：Take 30-50 grams of fresh dendrobium candidum, clean section or segment with clear water and clap and split, according to personal taste can stew chicken, Duck, bone etc..Pot time is slightly longer than usually a little.Enter wine：500 grams of fresh dendrobium candidum is taken, is rinsed with clear water Totally, taken in right amount sooner or later after 2 months with matrimony vine, American Ginseng, 1000 grams of white wine, immersion.Beat juice：Clean and press 10 with juicer:1 Add water to beat juice to eat, add honey taste more preferably, drink has Soboring-up liver-protecting after drinking before wine, mitigates headache effect, positive effect.

Health promotion effect of the dendrobium candidum for human body is known knowledge.Such as

Nourishing yin jin effect in relation to dendrobium candidum.《Chinese pharmacy dictionary》Maple bucket is claimed " specially to grow the gas-liquid of lung stomach, gas-liquid Rush prosperous, kidney water is spontaneous, " says that he is good at nourishing Yin and promoting production of body fluid, treats all diseases of Yin and fluid deficiency.Traditional Chinese medicine thinks that yin-fluid is lived for human life Dynamic material base, has and moistens body internal organs, brains bone, suppresses the dynamic effect of the high fire of sun, can maintain normally growth hair Educate and reproductive function activity.If human body YIN-fluid being insufficient, apathetic, dim complexion just occurs, eye does refreshing stagnant, waist and knee acid Soft, dizzy weak, mouth parched and tongue scorched, have sore throat, symptom, the maple bucket such as constipation are the key medicines of yin-nourishing, taking maple bucket can be from Fundamentally solve the problems, such as human body YIN-fluid being insufficient.

The effect of building up health of related dendrobium candidum.The stem of noble dendrobium has the function of nourishing yin and nourishing blood, the Qing Dynasty《Haigoushen》Say the stem of noble dendrobium Can kidney tonifying product essence, nourishing stomach-YIN, benefiting energy.Contain abundant polysaccharose substance in the stem of noble dendrobium.Experiment proves that dendrobium polysaccharide has enhancing The effect of immune function, can strengthen phagocytic activity of the peritoneal macrophage to chicken red blood cell.

The effect of strengthening the spleen and stomach of related dendrobium candidum.Taste have digestion diet, and intake essence of water and grain is with the weight of nutrition whole body Act on, be the source of nutrition, therefore the function of taste is depended primarily upon whether nutritional sufficiency.The stem of noble dendrobium is reinforcing stomach reg fluid medicine,《God Agriculture book on Chinese herbal medicine warp》、《Book on Chinese herbal medicine is new again》In it is on the books, he is referred to as " medicine for enterogastritis " to the people, is treatment epigastric pain, and upper abdomen is gone up painful common Medicine.Modern experimental confirms that the stem of noble dendrobium has good inhibiting effect to pathogenic bacteria-helicobacter pylori common in the spleen-stomach diseases, helps In the illness of the treatment Helicobacter pylori such as atrophic gastritis, superficial gastritis, duodenal ulcer, meanwhile, take orally the stem of noble dendrobium Decocting liquid can promote the secretion of gastric juice, strengthen the emptying ability of stomach, help digest.

Protect liver choleretic effect in relation to dendrobium candidum.The stem of noble dendrobium has preferable choleretic effect, and ancient Chinese medicine doctor all thinks " iron sheet maple Bucket " has the function that nourishing hepatic yin, is the key medicine of the various liver and gall for the treatment of simultaneously, available for livers such as treatment hepatitis, cholecystitis, gall stone Biliary diseases.Clearind deficient heat.Abnormal heat, shows as body fatigue and weak, dizzy, and flushed face, palpitaition are had palpitation, cough few phlegm, mistake Sleep one group of symptom such as forgetful, dark urine, tongue are red, arteries and veins is thin.《A Supplement to the Compendium of Materia Medica》、《Amplification on Materia Medica》" clearing stomach removes for record respectively Abnormal heat, " " abnormal heat in hemorrhoid stomach ".Compared with common heat-clearing medicine, the clear abnormal heat of the stem of noble dendrobium is entirely a kind of abnormal heat, be with the deficiency of Yin, With the characteristics of excess heat.Pharmacological evaluation shows that the stem of noble dendrobium can significantly reduce the heating peak value of excess heat rabbit and body temperature reaction refers to Number, shortens and plays the hot time.

The effect of strengthening muscles and bones of related dendrobium candidum.People entered after the middle age, " people's half of YIN QI consumed " (ZHU Dan-xi) human body Yin jin starts weak, and muscles and bones function is faded, and the stem of noble dendrobium can nourish yin-fluid, yin-fluid can lubricating joint, so as to reach strengthening tendons Strong bone, fluent joint, the effect for strengthening antirheumatic.Modern pharmacological studies have shown that the stem of noble dendrobium can improve stress ability, have well It is antifatigue, the effect of resist oxygen lack.In terms of promoting circulation, stem of noble dendrobium energy yin-nourishing, can make moist sering, blood vessel be expanded, so as to promote blood Liquid circulates.Test result indicates that the stem of noble dendrobium can significantly improve blood stasis symptom, cholesterolemia and triglyceride are reduced, is improved highly dense Lipoprotein cholesterol level is spent, cardiovascular and cerebrovascular disease can be treated.

Reduction blood glucose effect in relation to dendrobium candidum.The traditional Chinese medical science thinks, the generation of diabete (diabetes), mainly with kidney yin Void, the early heat of lung stomach is basic pathogenesis, using deficiency of both qi and yin as its pathological characteristic, stem of noble dendrobium function yin-nourishing ` clearing heat and moistening drynesses, since ancient times It is the medicine dedicated for treating diabetes.Clinical research shows that the stem of noble dendrobium can not only strengthen insulin active, while can significantly reduce blood Sugar level, makes blood sugar recovery normal.

Function of tumor inhibition in relation to dendrobium candidum.The stem of noble dendrobium to Human Lung Cancer, oophoroma and progranulocyte leukemia Some cells have killing effect, have stronger antitumor activity.Clinical time shows that the auxiliary that the stem of noble dendrobium is used for malignant tumour is controlled Treat, the symptom of tumor patient can be improved, mitigate the side effect of Radiotherapy chemotherapy, strengthen immunity, improves life quality, extends existence Time.

Bright eyes effect in relation to dendrobium candidum.The traditional Chinese medical science thinks that the vital essence of the vital organs of the human body is all by arteries and veins, noting in eye Eyeball, and liver " have one's ideas straightened out in mesh " eyes physiological and pathological in liver the moon essence it is vigorous whether it is in close relations, the stem of noble dendrobium have enriching yin support purpose Function, by good merchantable brand of the ancient Chinese medicine doctor as eye-protecting.Modern efficacy of a drug research confirmation, the stem of noble dendrobium ophthalmology common to prevention the elderly Disease-cataract not only has retarding action, can keep lenticular transparency 36 points eight percent, and galactolipin Caused enzymatic activity anomalous variation has suppression or role of correcting.

Nourishing skin effect in relation to dendrobium candidum.After human body enters midlife, since internal yin-fluid is increasingly reduced, from And skin aging is accelerated, it is allowed to blackening or crumple.The stem of noble dendrobium contains the lymphatic temperament of volume, has moist nutrition to make to human skin With.

The effect of promoting longevity of related dendrobium candidum.《Sheng Nong's herbal classic》The stem of noble dendrobium is classified as with " macrobiosis of making light of one's life by commiting suicide " effect Commodity medicine.Modern research shows that the stem of noble dendrobium contains various trace elements, these good health and a long life of trace element for human body have Close relationship, the stem of noble dendrobium are more extensive to the anti-aging effects of human body than general medicine to the anti-aging effects of human body, more comprehensively.

In addition, various classical traditional Chinese medicine treatises are all documented in detail dendrobium candidum effect.Such as《Compendium of Materia Medica》 Record dendrobium candidum：Gas under eliminating impediment, tonifying five zang organs consumptive disease win thin, strong the moon strengthening the essence.Long term usage, thick stomach, mend in never enough, flat stomach gas, Long muscle, by skin heat symptoms caused by an exopathgen miliaria gas, the cold numbness of foot knee pain is weak, determines intelligence except frightened, macrobiosis of making light of one's life by commiting suicide.QI invigorating is good for sun, by skin wind except heat Numbness, cold, kidney tonifying benefit power long in bone.Smelting fever spontaneous perspiration, the interior plug of ulcer apocenosis.《Sheng Nong's herbal classic》Record dendrobium candidum：In main wound, Eliminating impediment, lower gas, tonifying five zang organs consumptive disease, thin thin, Qiang Yin.Long term usage thickness stomach, make light of one's life by commiting suicide, macrobiosis.《This warp》Record dendrobium candidum：It is sweet in flavor, It is flat.In main wound, eliminating impediment, lower gas, tonifying five zang organs consumptive disease is thin and weak, Qiang Yin, long term usage thickness stomach.《Detailed outline》：It is sweet light micro- salty, control fever certainly Sweat, the interior plug of ulcer apocenosis.《Detailed outline is picked up any lost article from the road》Record dendrobium candidum：Clearing stomach removes abnormal heat, the sixth of the twelve Earthly Branches strain of promoting the production of body fluid, with generation tea, appetizing is strong Spleen.Wind, energy town saliva phlegm, sweet virtue sending down abnormally ascending are treated in arresting convulsion.

The eating method of dendrobium candidum is well known.

1. fresh food.In the age that former no antibiotic is brought down a fever, fever of the fresh dendrobium candidum to child has special efficacy Effect, and without the side effect of antibiotic.To fever caused by various fevers, as long as fresh dendrobium candidum is mashed and boiling water Swallow, or direct chewing, or decocted and taken with boiling water, you can play the role of bringing down a fever, it can also be used into ruling toothache caused by fire of deficiency type by men.

2. make tea.Old wild goose mountain Tiepi Fengdou ground into end, 3~5 grams/person/day, boiling water Instant Drinks continue to have special efficacy in 30 days. Tiepi Fengdou can be added boiling 30 minutes used above, be recycled edible, the same day is eaten up, and is eaten up together with slag.Can heat-clearing enriching yin, It is antifatigue.

3. meals are eaten.Tiepi Fengdou can be added in diet, such as stewed chicken, stew pigeon.Two refer to below： 1) Tiepi Fengdou congee：5 grams of Tiepi Fengdou, obstructs 50 grams of rice, appropriate rock sugar.2) cordyceps sinensis Tiepi Fengdou soup：Raw a red-spotted lizard of breathing out is a pair of, the worm summer in winter Grass 5,5 grams of Tiepi Fengdou, 200 grams of thin pork, one jiao of dried orange peel.(curative effect) nourishing Yin and promoting production of body fluid is nourishing liver and kidney, and old children is all suitable.

4. the clearing stomach of maple bucket is drunk.10 grams of [raw material] old wild goose mountain Tiepi Fengdou, 12 grams of caulis bambusae in taenian, 30 grams of reed root, public 15 grams of English, Fructus Aurantii 10 grams, 15 grams of ophiopogon, 6 grams of peppermint, 12 grams of Radix Paeoniae Alba, 6 grams of radix glycyrrhizae.200 milliliters of [usage] decocting, once in the morning and once at night, warmly takes before meals. [curative effect] light refrigerant profit, regulating qi-flowing for relieving pain.Chronic superficial gastritis, gastric ulcer partial heat person.

5. infusing drugs in wine is drunk.Macrobiosis Tiepi Fengdou wine：250 grams of Tiepi Fengdou, 60 grams of radix rehmanniae recen, 30 grams of radix achyranthis bidentatae, the bark of eucommia 20 Gram, 20 grams of Radix Salviae Miltiorrhizae, 10 liters of white wine.[curative effect] lumbocrural pain, body is tired powerless, the disease such as rheumatism numbness.Kidney tonifying, strengthening the bones and muscles, except overworked.

6. educate cloudy nourshing kidney soup (1 people's deal) material：Each 15 grams of cordyceps sinensis, dendrobium candidum, Radix Paeoniae Alba, dogwood fruit, mulberry fruit, Each 25 grams of the fruit of glossy privet, Eclipta prostrata, cultivated land.Pot method：4 bowls of clear water is decocted to 1 bowl.Effect：Cordyceps not only nourshing kidney but also tonifying liver, plus iron sheet The stem of noble dendrobium, Radix Paeoniae Alba, mulberry fruit, dogwood fruit etc. are all the Chinese medicine of enriching yin, are most suitable for deficiency of Yin personage's filling liver kidney and are used.

7. American ginseng dendrobium tea：5 grams of [raw material] American Ginseng, 30 grams of old wild goose mountain Tiepi Fengdou.[preparation method] first picks American Ginseng It is miscellaneous, clean, dry or dry, be cut into medicine materical crude slice, be put into larger container, it is spare.By old wild goose mountain Tiepi Fengdou, earth pot is put into, is added 200ml water, after being boiled by fire, uses small fire mulling instead 30 minutes, with clean filtered through gauze, removes slag, and collects filtered juice and contains into entering to have west In the container of American ginseng medicine materical crude slice, it is capped vexed 15 minutes, you can drink.(or American Ginseng, the stem of noble dendrobium are ground into fine powder and wash water open on behalf of the tea With 3~5 grams every time, daily 1~2 time.) [eating method] work as tea, at each 2 times of upper and lower noon, drink again and again.Take on behalf of the tea, the same day is eaten up. [effect] tonifying-Yin and nourishing-stomach, cough-relieving of promoting the production of body fluid.We are suitable for the concurrent stomatitis patient of stomach-Yin deficiency real heat type leukaemia.[review] the West Join cool in nature, sweet in flavor, slight bitter, is good at tonifying Qi enriching yin, relieves inflammation or internal heat and promote the production of body fluid.Modern pharmacological research proves that American Ginseng can improve the anti-of body Sick ability, suppresses the growth of cancer cell.Stem of noble dendrobium function nourishing stomach-YIN, promote the production of body fluid, and clinical research data shows, the stem of noble dendrobium to cancer chemotherapy, Polydipsia caused by hindering Tianjin and syndrome of yin deficiency and depletion of fluid after radiotherapy, the dry, constipation of dry throat tongue etc. truly have effect.Two taste compatibilities are dietotherapy Tea-drinking, has obvious tonifying-Yin and nourishing-stomach, cough-relieving effect of promoting the production of body fluid, concurrent stomatitis patient has preferably to stomach-Yin deficiency heat type leukaemia Adjuvant treatment effect, particularly suitable youngsters and children and old leukaemic.

8. steamed stem of noble dendrobium spiral shell：Raw material：Blue or green 1500 grams of spiral shell (stone spiral shell), 9 grams of pig fillet, 6 grams of the stem of noble dendrobium.Condiment：The a little preparation method of salt： 1) blue or green spiral shell is spat mud, is cleaned, and is cooked by scalding, picked up with boiling water.Continue to employ after soup filtering.2) choose spiral shell meat, cleaned with light salt brine, drained, Load stew cup.Pig fillet is cut into even block, dirty with the boiling water blood that flies to.3) spiral shell juice is first about stewed after twenty minutes with the stem of noble dendrobium with a pannikin, is removed The dregs of a decoction are removed, filtering concoction is stand-by.4) concoction is poured into stew cup, then pig fillet is put on the spiral shell flesh noodles in cup, it is small about to stew 1 Shi Hou, calls in salt, you can edible.Characteristic：Delicious micro-sweet.Effect：The stem of noble dendrobium is closed with blue or green spiral shell, pork and stewed, energy nourishing Yin and moistening dryness, and tonneau is small Just, quench one's thirst Li Shui, to thin and weak, constipation of quenching one's thirst, cough caused by dryness, drunk does not wake up.

The soup 9. yin-nourishing is quenched one's thirst：Material：6~9 grams of the stem of noble dendrobium, asparagus fern, radix polygonati officinalis, adenophora tetraphylla, sealwort, cultivated land, Chinese yam, Poria cocos each 12 Gram, 5 grams of dried orange peel.Usage：It can arbitrarily drink and add lean meat or appropriate chicken, add eight bowls of boiling water is same to stew three hours.At present salt seasons food.Work( Effect：Liver stomach-Yin is excessively caused to hinder all diseases to diabetes diabetes, tobacco and wine quite effective.

10. stem of noble dendrobium Cassia electuary：Material：The stem of noble dendrobium, American ginseng are 30~50 grams each, matrimony vine, chrysanthemum, Semen Cuscutae, semen astragali complanati, female Loyal son, each 60 grams of cassia seed plantain seed, 60~80 grams of Radix Salviae Miltiorrhizae.Usage：Medicine-feeding grinds to the powder altogether.Twice a day, every time 3~5 grams.Open Mixing in water for oral taking.It is continuous take more than three months it is better.Effect：To the hypertension caused by the deficiency of liver-yin and kidney-yin, glaucoma, cataract etc. Disease is effective.

11. tea beverage：Radix polygonati officinalis, Radix Glehniae, the stem of noble dendrobium, each 15 grams of ophiopogon, five pieces of dark plum, decocting takes juice, adds rock sugar appropriate generation Tea-drinking is used, and can control consumption of YIN caused by febrile disease, or the more caused drys of summer perspiration think drink, are hard and dry.12. 15 grams of Radix Glehniae, the dried rhizome of rehmannia, wheat Each 10 grams of winter, the stem of noble dendrobium, appropriate rock sugar, is decocted in water for oral dose, and lacks suitable for the body fluid after hyperpyrexia disease, dry throat, loses the appetite, stool It is constipated, the disease such as difficult urination.

Tiepi Fengdou usage and dosage usually have：1. use low temperature breaking-wall cell polishing：It is ground into more than 200 mesh thin Powder (is sure not to beat powder with high speed fever type common grinder, molecule of pharmaceutical is destroyed because producing high temperature in shattering process).Take daily 3 grams of Tiepi Fengdou fine powder, point secondary uses warm water delivery service sooner or later【Tonifying Qi is such as needed, can also add a little American Ginseng or Ginseng Root Powder With clothes】.Recommend this method best results suitably long-term use of.2. the ancient wide soup of method is decocted long：Take 5~6 grams of Tiepi Fengdou former material (two days Amount), add 1.5 liters of water to be stewed with marmite.Take after boiling twenty four hours for the first time, taken after boiling eight hours for the second time, third time Take after boiling eight hours, take soup and Tiepi Fengdou slag together after boiling eight hours for the 4th time.Good Tiepi Fengdou is clear after boiling Fragrant matter is tender, slag is few, viscosity is high, and mouthfeel is clear and oiliness, can not have to add any flavouring and directly drink.It is suitable long-term use of 【Tonifying Qi is such as needed, can also add a little American Ginseng or ginseng】.3. 3~5 grams of Tiepi Fengdou former materials are taken, and chicken and duck etc. together stew by slow boiling 100 minutes, connect slag and eat.

The present invention provides a kind of product for strengthen immunity particularly health food, and this health food has There is excellent biological effectiveness.The present inventor is surprisingly, it was found that dendrobium candidum and Paecilomyces hepiali chen using specified quantitative Granule made of bacterium powder is extracted has unexpected advantageous feature.

Embodiment

The present invention can be further described by the following examples, however, the scope of the present invention and unlimited In following embodiments.One of skill in the art, can be with it is understood that on the premise of without departing substantially from the spirit and scope of the present invention Various change and modification are carried out to the present invention.The present invention carries out the material and test method that are arrived used in experiment general And/or specific description.Although for realize many materials used in the object of the invention and operating method be it is known in the art that But the present invention is still described in detail as far as possible herein.Following embodiments further illustrate the present invention, rather than limit this hair It is bright.

Hereafter preparation process is for the purpose of citing, and the comparability based on each citing and make some specific description, Those skilled in the art can therefrom summarize to obtain particle of the present invention preparation for strengthen immunity completely according to existing knowledge Method.In the various examples for preparing the particle that the present invention is used for strengthen immunity below, if not otherwise indicated, every batch of adds Work amount is calculated as 1kg with dendrobium candidum inventory.It is that iron sheet is converted to the amount of every bag of packing if not otherwise indicated in packing The amount of the gauge 1g of lime dry measure used in former times is dispensed into bottle.

Embodiment 1：Prepare the particle for strengthen immunity

Dispensing：

Dendrobium candidum	1000g
		Peacilomyce hepiahi bacterium powder	1500g
Glucose	2600g

(3) weigh pharmaceutic adjuvant and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, the wet system of softwood Grain, it is dry, to obtain the final product.The granule is dispensed into aluminum plastic compound membrane bag, is sealed.Moisture 4.6%.

Embodiment 2：Prepare the particle for strengthen immunity

Dispensing：

Dendrobium candidum	1000g
		Peacilomyce hepiahi bacterium powder	1200g
Glucose	2800g

(3) weigh pharmaceutic adjuvant and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, the wet system of softwood Grain, it is dry, to obtain the final product.The granule is dispensed into aluminum plastic compound membrane bag, is sealed.Moisture 3.8%.

Embodiment 3：Prepare the particle for strengthen immunity

Dispensing：

Dendrobium candidum	1000g
		Peacilomyce hepiahi bacterium powder	1800g
Glucose	2200g

(3) weigh pharmaceutic adjuvant and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, the wet system of softwood Grain, it is dry, to obtain the final product.The granule is dispensed into aluminum plastic compound membrane bag, is sealed.Moisture 5.3%.

Embodiment 4：Prepare the particle for strengthen immunity

Dispensing：

Dendrobium candidum	1000g
		Peacilomyce hepiahi bacterium powder	1000g
Glucose	3000g

(3) weigh pharmaceutic adjuvant and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, the wet system of softwood Grain, it is dry, to obtain the final product.The granule is dispensed into aluminum plastic compound membrane bag, is sealed.Moisture 4.4%.

Embodiment 5：Prepare the particle for strengthen immunity

Dispensing：

Dendrobium candidum	1000g
		Peacilomyce hepiahi bacterium powder	2000g
Glucose	2000g

(3) weigh pharmaceutic adjuvant and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, the wet system of softwood Grain, it is dry, to obtain the final product.The granule is dispensed into aluminum plastic compound membrane bag, is sealed.Moisture 4.8%.

Embodiment 11：Prepare the particle for strengthen immunity

Respectively according to the formula and preparation method of embodiment 1, different is only sucrose, the breast that glucose therein is replaced with to equivalent Sugar, fructose or mannitol, obtain the granule of four batches.Moisture is in the range of 3.8%~5.1%.

Embodiment 12：Prepare the particle for strengthen immunity

According to the formula and preparation method of embodiment 1, different is only to replace with the amount of peacilomyce hepiahi bacterium powder therein 250g and 500g, obtains the granule of 2 batches.Moisture is in the range of 3.5%~5.2%.

Embodiment 13：Prepare the particle for strengthen immunity

According to the formula and preparation method of embodiment 1, different is only to replace with the amount of peacilomyce hepiahi bacterium powder therein 2500g and 5000g, obtains the granule of 2 batches.Moisture is in the range of 3.7%~5.3%.

With reference to the method for following experiments example 2, example 2 above -5 and embodiment 12 and embodiment 13 are prepared Whole granules carry out dendrobium officinale granula strengthen immunity function animal experiment.It turns out that：Four of embodiment 2-5 Granula and the result of 1 granule of embodiment are essentially identical, and showing to animal has the function of the strengthen immunity (number respectively tested There is difference according to micro-, but statistical result is identical)；But four kinds of particles that embodiment 12 and embodiment 13 are prepared Result of the agent with 1 granule of embodiment differs, and statistical result shows that strengthen immunity is not presented to animal for they Function, and bat moth in the lower sample of peacilomyce hepiahi bacterium powder or 13 liang of samples of embodiment in 12 liang of samples of embodiment The higher sample of Paecilomyces varioti bacterium powder, it is bigger with the significant difference of 1 granule result of embodiment.

Test example 1：Stability test

The adenosine included in granule of the present invention is the index component of peacilomyce hepiahi bacterium powder.Adopted in this test example With《Health food is examined and assessment technique specification》(version in 2003) contained method of page 300, measures various forms of the present invention Product in adenosine stability.

Dry extract, embodiment 1-5 steps (2) gained granule, embodiment 11 obtained by the step of example 1 above -5 (2) Gained granule, makes them use aluminum-plastic composite membrane bag hermetic package respectively, is placed 3 months at a temperature of they are placed in 38 DEG C, surveys Residual content when adenosine in fixed each sample during March was relative to the sample 0 month.

As a result：Adenosine residual content after each dry extract each leisure high-temperature treatment March obtained by embodiment 1-5 steps (2) In the range of 88.4%~91.1%, the adenosine remnants after each granule each leisure high-temperature treatment March obtained by embodiment 11 contain Amount is in the range of 88.8%~91.3%, the gland after granule obtained by embodiment 1-5 steps (2) each comfortable high-temperature treatment March Glycosides residual content is in the range of 97.6%~100.2%.Typically, adenosine residual content is low after this hot test processing Those skilled in the art have been generally acknowledged that product is unqualified when 90%.

Test example 2：Dendrobium candidum particle strengthen immunity function animal experiment

1 gained granule of embodiment used below is tested.

1. material and method

1.1 sample：

Dendrobium candidum particulate samples are brown yellow granule.Daily recommended amounts of being grown up are 6000mg/60kg.BW, and when experiment is used Distill water as solvent and prepare tested material, it is stored refrigerated.

1.2 experimental animal：The Kunming mouse 160 provided by Sichuan Provincial Academy of Traditional Chinese Medicine Experimental Animal Center, entirely Female, weight 18-22g, production licence number is 2013-19 SPF grades of SCXK (river)；Feed resource is in Sichuan Academy of Medical Sciences Institute of lab animals of People's Hospital, Sichuan Prov., production licence number are SCXK (river) 2010-01.Experimental animal room is barrier system System, is SYXK (river) 2011-043 using credit number.20-25 DEG C of temperature, relative humidity 40-70%.

1.3 dosage select to give mode with tested material：If 1000mg/kg.BW, 2000mg/kg.BW, 3000mg/kg.BW Three dosage groups (10 times, 20 times, 30 times that are respectively equivalent to human body recommended intake), weigh respectively 12.50g, 25.00g, 37.50g tested materials add distilled water to be mixed to 250ml, are finished and match somebody with somebody again, separately set distilled water negative control group, every group of 40 animals.Point To be immunized one group, two groups, three groups, four groups, one of which is used for Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment；Two groups of use Tested in NK cytoactive detections and lymphocyte transformation；Three groups be used for antibody-producting cell experiment, serum hemolysin measure and Tardive allergy is tested；Four groups are used for mouse carbonic clearance and test.Mouse presses 20ml/kg.BW weight once oral gavage, extremely It is few continuously to give 30 days, claim a weight weekly, adjust gavage volume.

1.4 key instruments and reagent：100 μ l micro syringes, CO2gas incubator, electronic analytical balance, 723 points Light photometer, centrifuge, 2010 type microplate reader, operating theater instruments, SRBC, Hank ' s liquid, DNFB, S_ABuffer solution, agarose, India Prepared Chinese ink, Na₂CO₃, RPMI1640 etc..

1.5 test method

1.5.1 internal organs/weight ratio measurement：Animal is put to death after weighing during off-test, takes spleen and thymus gland in electronics Weigh on assay balance, and calculate dirty/body ratio.

1.5.2 delayed allergy (DTH) (vola pedis thickens method)：2% hematocrit SRBC is injected to mouse peritoneal (0.2ml/ is per mouse) sensitization after 4 days, measures left back vola pedis thickness, and 20% (v/v) SRBC (20 then is subcutaneously injected in measuring point μ l/ are per mouse), 24h measures left back vola pedis thickness after injection, and same position measurement three times, is averaged, to attack front and rear vola pedis Thickness difference (swelling degree of the paw) represents the degree of DTH.

1.5.3 the mouse lymphocyte conversion test (mtt assay) of ConA inductions：It is sterile to take spleen, equipped with sterile Hank ' s In liquid plate, spleen is ground with tweezers, cell suspension is made, 200 mesh screens, are washed 2 times with Hank ' s liquid, every time centrifugation 10min (1000r/min), then takes precipitation to be suspended in the complete culture solution of lmL, platform phenol orchid dyeing counting (should 95% with On).Cell concentration is adjusted to 3 × 10⁶A/ml, then divides holes to add in 24 well culture plates cell suspension, per hole 1m1, One hole adds 75 μ lConA liquid, and another hole compares, and puts 5%CO₂37 DEG C of culture 72h of incubator.Culture terminates preceding 4h, is gently inhaled per hole Supernatant 0.7ml is removed, 0.7ml is added and is free of the RPMI1640 nutrient solutions of calf serum, while add 50 μ l/ of MTT (5mg/ml) Hole continues to cultivate 4h, after culture, 1ml acid isopropyl alcohol is added per hole, purple crystal is completely dissolved, is then dispensed into 96 Well culture plate, 3 parallel holes are done per hole, and with 2010 type microplate reader, OD value is measured with 570nm wavelength.

1.5.4 antibody-producting cell detection (Jerne improves slide methods)：Every mouse peritoneal injects 2% hematocrit SRBC 0.2ml, the mouse cervical dislocation after being immunized 5 days are put to death, and are taken out spleen and are placed in the plate for filling Hank ' s liquid, grind spleen It is dirty, cell suspension is made, through 200 mesh sieve net filtrations, centrifuges (1000r/min) 10min, is washed 2 times with Hank ' s liquid, finally will be thin Born of the same parents are suspended in 5ml RPMI1640 nutrient solutions, platform phenol orchid dyeing counting (should be more than 95%), and cell concentration is adjusted to 5 ×10⁶A/ml, is made splenocyte suspension.Agarose is configured to 1% aqueous solution, 30min is boiled in water-bath, with the double concentration of equivalent Hank ' s liquid mixes, and dispenses small test tube, often pipe 0.5ml, then adds 10% (using SA buffers) hematocrit SRBC, 50 μ into pipe L, each 20 μ l of splenocyte suspension do two Duplicate Samples, after rapid mixing, are poured on agarose thin layer slide, treat agar solidification Afterwards, horizontal buckle of slide is placed on horse, is put into CO2gas incubator and is incubated 1.5h, complement is then added to glass frame In groove, continue to incubate 1.5h, count hemolysis plaque number.Prepared by complement is collection guinea pig blood, isolates serum (at least 5 globefish The pooled serum of mouse), 1ml hematocrits SRBC is added in 5ml guinea pig serums, 4 DEG C of refrigerators place 30min, often vibration, centrifugation Supernatant is taken, is dispensed, -70 DEG C of preservations.Used time is with SA buffer solutions by 1: 15 dilution.

1.5.5 serum hemolysin measure (Hemagglutination Method)：Every mouse peritoneal injection 2%SRBC 0.2ml is immunized, and continues to fill After stomach 5 days, extract eyeball and take blood when placement 1 is small, to be peeled off, 2000r/min is centrifuged 10 minutes, collects serum, is used in centrifuge tube Serum is made doubling dilution by physiological saline, and every part of 12 holes of dilution, the serum of different dilution factors are placed in blood-coagulation-board, per 100 μ of hole L, adds 100 μ l of 0.5%SRBC suspensions, mix, put wet box it is 37 DEG C 3 small when after observation as a result, record per hole aggegation degree. Calculating antibody product.

1.5.6 mouse carbonic clearance test：India ink in mouse tail vein injection with 5 times of normal saline dilutions, is pressed Timing immediately after the injection of 0.1ml/10g prepared Chinese ink, the 2nd, 10min takes 20 μ l of blood from angular vein clump respectively after prepared Chinese ink is injected, and adds To 2mlNa₂CO₃In solution, with Na₂CO₃Solution makees blank control, and OD value is measured at 600nm with 723 spectrophotometers. Mouse is put to death after taking blood, takes liver, spleen to weigh, calculates phagocytic index.

1.5.7 Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment (half intracorporal method)：Every mouse peritoneal injection NS 20% chicken erythrocyte suspension 1ml after washing 3 times, after 30 minutes, cervical dislocation is put to death, and is fixed on mouse plate, center is cut Abdominal skin is opened, through abdominal cavity saline injection 2ml, rotates mouse plate 1 minute, suctions out abdominal cavity washing lotion 1ml, mean droplet is in 2 loads On slide, wet box 37 DEG C is put 30 minutes, take out and rinse in physiological saline, dry, is fixed, 4%Giemsa PBS are dyed 3 minutes, Distilled water rinses airing, microscopy.Calculate phagocytic percentage and phagocytic index.

1.5.8 NK cytoactive detections (lactate dehydrogenase L DH determination methods)：Cervical dislocation puts to death animal, takes out spleen, Tear up, after crossing 200 mesh sieve nets, washed 3 times with Hank ' s liquid, each 1000r/min centrifuges 10min, takes cytoplasm, and sterilize water-splitting Red blood cell, platform phenol orchid dyeing counting (should be more than 95%), 2 × 10 are made into complete 1640 culture medium⁷A/ml cell suspensions. 300 μ l are taken to be placed in 96 well culture plates the cell suspension of each mouse, per 100 μ l of hole, adding target cell per hole, (YAC-1 is thin Born of the same parents, 4 × 10⁵A/ml) 100 μ l, while do target cell Spontaneous release hole (100 μ l+ nutrient solutions of target cell, 100 μ l) and maximum is released Discharge hole (100 μ l+2.5%Triton of target cell, 100 μ l) each 3 hole, 37 DEG C of 5%CO₂Cultivate 4 it is small when, take out 1500r/min from Heart 5min.Each 100 μ l of hole supernatant are placed in another culture plate, 100 μ l matrix liquids is added per hole, adds after ten minutes 30 μ l of 1mol/L HCL terminate reaction, and OD values are measured at 490nm, calculate NK cytoactive rates.

1.6 test datas count：Test data is handled using 10.0 for Windows software kits of SPSS.Control group with For the data of dosage group through homogeneity test of variance, variance is neat, carries out variance analysis, if P values are less than 0.05, then with Dunnett methods into Row compares two-by-two；If heterogeneity of variance, carry out data conversion, it is still uneven, use rank sum test instead, as P values be less than 0.05, then use Dunnett ' s T3 methods are compared two-by-two.

1.7 result judgement：In cellular immune function, humoral immune function, monocytes/macrophages function, NK cytoactives Any two aspect results are positive in terms of four, can determine that the given the test agent has strengthen immunity function.

2. result

The influence of 2.1 dendrobium candidum Granules on Mouse weight

The original body mass of 1 dendrobium candidum particle each group mouse of table

The mid-term weight of 2 dendrobium candidum particle each group mouse of table

The end weight of 3 dendrobium candidum particle each group mouse of table

The influence of 4 dendrobium candidum Granules on Mouse weight gain of table

By table 1-4 as it can be seen that dendrobium candidum particle be immunized one group, two groups immune, immune three groups and immune four groups of animals just Initial body weight is compared with negative control group, and through homogeneity test of variance, variance is neat (P ＞ 0.05), and the results of analysis of variance (P ＞ 0.05) it is balanced, to illustrate the original body mass between each group animal and negative control group.One, two, three, four group of each dosage is immunized Relatively nothing is notable compared with negative control group for the growth of mouse weight during group animal experiment mid-term, the weight in latter stage and experiment Sex differernce (P ＞ 0.05).

The influence of 2.2 dendrobium candidum Granules on Mouse internal organs/weight ratio

The influence of 5 dendrobium candidum Granules on Mouse internal organs of table/weight ratio

By table 5 as it can be seen that continuous gavage is after 30 days, spleen/weight ratio, the thymus gland/weight ratio of three dosage group animals Compared with negative control group, it is statistically analyzed, difference that there are no significant (P ＞ 0.05).

The influence of 2.3 dendrobium candidum Granules on Mouse cellular immune functions

2.3.1 the influence of dendrobium candidum Granules on Mouse delayed allergy (DTH)

The influence of 6 dendrobium candidum Granules on Mouse delayed allergy (DTH) of table

By table 6 as it can be seen that continuous gavage is after 30 days, the toes swelling of three dosage group animals is compared with negative control group, warp Statistical procedures, there was no significant difference (P ＞ 0.05).

2.3.2 the influence for the mouse lymphocyte conversion test that dendrobium candidum particle induces ConA

The influence for the mouse lymphocyte conversion test that 7 dendrobium candidum particle of table induces ConA

By table 7 as it can be seen that continuous gavage mouse is after 30 days, the lymphopoiesis ability and feminine gender of three dosage group animals are right Compare, be statistically analyzed according to group, there was no significant difference (P ＞ 0.05).

Dendrobium candidum Granules on Mouse test for celluar immunity result is negative.

The influence of 2.4 dendrobium candidum Granules on Mouse humoral immunities

2.4.1 influence of the dendrobium candidum particle to antibody-producting cell

The influence of 8 dendrobium candidum Granules on Mouse antibody-producting cell of table

By table 8 as it can be seen that continuous gavage is after 30 days, the hemolysis plaque number of three dosage group animals compared with negative control group, It is statistically analyzed, there was no significant difference (P ＞ 0.05).

2.4.2 the influence of dendrobium candidum Granules on Mouse serum hemolysin

The influence of 9 dendrobium candidum Granules on Mouse serum hemolysin of table

By table 9 as it can be seen that continuous gavage is after 30 days, the antibody product of three dosage group animals is compared with negative control group, warp Statistical procedures, middle and high dosage group animal have significant difference (P ＜ 0.05, P ＜ 0.01).

Dendrobium candidum Granules on Mouse test for humoral immunity result is positive.

The influence of 2.5 dendrobium candidum Granules on Mouse monocytes/macrophages phagocytic functions

2.5.1 the influence of dendrobium candidum Granules on Mouse monocytes/macrophages carbonic clearance function

The influence of 10 dendrobium candidum Granules on Mouse monocytes/macrophages carbonic clearance function of table

By table 10 as it can be seen that continuous gavage is after 30 days, the carbonic clearance ability of three dosage group animals and a negative control group phase Than being statistically analyzed, there was no significant difference (P ＞ 0.05).

2.5.2 the influence of dendrobium candidum Granules on Mouse macrophage phagocytosis chicken red blood cell ability

11 dendrobium candidum Granules on Mouse macrophage of table swallows the influence of chicken red blood cell ability

By table 11 as it can be seen that continuous gavage is after 30 days, the phagocytic rate and phagocytic index and negative control of three dosage group animals Group is compared, and is statistically analyzed, and there was no significant difference (P ＞ 0.05).

Dendrobium candidum Granules on Mouse monocytes/macrophages phagocytic function test result is negative.

The influence of 2.6 dendrobium candidum Granules on Mouse NK cytoactives

The influence of 12 dendrobium candidum Granules on Mouse NK cytoactives of table

By table 12 as it can be seen that continuous gavage is after 30 days, the NK cytoactives of three dosage group animals compared with negative control group, It is statistically analyzed, low, high dose group animal has significant difference (P ＜ 0.01, P ＜ 0.01).

Dendrobium candidum Granules on Mouse NK cell activity assays result is positive.

3. brief summary

Dendrobium candidum particle continuous gavage mouse is after 30 days, and to the weight of mouse, internal organs/weight ratio has no significant effect, It is negative to the test for celluar immunity result of mouse, it is negative to the monocytes/macrophages phagocytic function test result of mouse, to mouse Test for humoral immunity result it is positive；It is positive to the NK cell activity assays result of mouse.As can be seen here：Dendrobium candidum particle pair Animal has the function of strengthen immunity.

Industrial applicability

The present invention has using the extracted manufactured granule of dendrobium candidum and peacilomyce hepiahi bacterium powder of specified quantitative The advantageous feature that people expects, such as seek peace physicochemical property with excellent biological characteristics.

Claims

1. a kind of particle for strengthen immunity, its be made of dendrobium candidum and peacilomyce hepiahi bacterium powder are extracted, The weight ratio of dendrobium candidum and peacilomyce hepiahi bacterium powder is 100：100~200；The particle is prepared according to following steps：

(3) weigh pharmaceutic adjuvant glucose and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, softwood is wet Granulation, it is dry, to obtain the final product；Wherein the weight ratio of dendrobium candidum medicinal material and glucose is 100：200~300.

2. the particle according to claim 1 for strengthen immunity, the wherein weight of dendrobium candidum and peacilomyce hepiahi bacterium powder Amount is than being 100：120~180.

3. the particle according to claim 1 for strengthen immunity, the wherein weight of dendrobium candidum and peacilomyce hepiahi bacterium powder Amount is than being 100：150.

4. the particle according to claim 1 for strengthen immunity, wherein the dry extract is prepared into according to following method Arrive：

Weigh dendrobium candidum, peacilomyce hepiahi bacterium powder, add 20 times amount water, extract 2 times, every time 1 it is small when, merge extracting solution；

Relative density when extracting solution is concentrated under reduced pressure into 60 DEG C under conditions of 70-80 DEG C of temperature, 0.06 ~ 0.08MPa of vacuum For 1.20-1.25, medicinal extract is obtained；

Medicinal extract is dried in vacuo under conditions of 60~70 DEG C of temperature, 0.06~0.08MPa of vacuum, is crushed, is obtained dry extract.

5. the particle according to claim 1 for strengthen immunity, wherein dendrobium candidum medicinal material and the weight for adding glucose Amount is than being 100：220~280.

6. the particle according to claim 1 for strengthen immunity, wherein dendrobium candidum medicinal material and the weight for adding glucose Amount is than being 100：230~270.

7. the particle according to claim 1 for strengthen immunity, wherein dendrobium candidum medicinal material and the weight for adding glucose Amount is than being 100：260.

8. the particle according to claim 1 for strengthen immunity, it has the moisture less than 5%.

9. preparing the method for the particle for strengthen immunity, this method comprises the following steps：

(3) weigh pharmaceutic adjuvant glucose and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, softwood is wet Granulation, it is dry, to obtain the final product,

Wherein, the weight ratio of dendrobium candidum and peacilomyce hepiahi bacterium powder is 100：100 ~ 200, dendrobium candidum medicinal material and glucose Weight ratio be 100：200~300.

10. method according to claim 9, it comprises the following steps：

(1) dry extract is prepared：Weigh dendrobium candidum, peacilomyce hepiahi bacterium powder, add 20 times amount water, extract 2 times, every time 1 it is small when, Merge extracting solution；

(2) it is opposite when extracting solution is concentrated under reduced pressure into 60 DEG C under conditions of 70-80 DEG C of temperature, 0.06 ~ 0.08MPa of vacuum Density is 1.20-1.25, obtains medicinal extract；Medicinal extract vacuum under conditions of 60~70 DEG C of temperature, 0.06~0.08MPa of vacuum is done It is dry, crush, obtain dry extract；

(3) weigh pharmaceutic adjuvant glucose and dry extract is uniformly mixed, add appropriate purified water, stir softwood processed, softwood is wet Granulation, it is dry, to obtain the final product.

11. method according to claim 9, wherein there is the obtained particle for strengthen immunity the moisture less than 5% to contain Amount.

12. method according to claim 9, the wherein weight ratio of dendrobium candidum and peacilomyce hepiahi bacterium powder are 100：120~ 180。

13. method according to claim 9, the wherein weight ratio of dendrobium candidum and peacilomyce hepiahi bacterium powder are 100：150.

14. method according to claim 9, wherein dendrobium candidum medicinal material and the weight ratio for adding glucose are 100：220~ 280。

15. method according to claim 9, wherein dendrobium candidum medicinal material and the weight ratio for adding glucose are 100：230~ 270。

16. method according to claim 9, wherein dendrobium candidum medicinal material and the weight ratio for adding glucose are 100：260.