CN104846555A - Preparation method of gelatin nanofiber membrane capable of synergistically releasing vitamin A and vitamin E - Google Patents
Preparation method of gelatin nanofiber membrane capable of synergistically releasing vitamin A and vitamin E Download PDFInfo
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- CN104846555A CN104846555A CN201510227786.5A CN201510227786A CN104846555A CN 104846555 A CN104846555 A CN 104846555A CN 201510227786 A CN201510227786 A CN 201510227786A CN 104846555 A CN104846555 A CN 104846555A
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Abstract
The invention relates to a preparation method of a gelatin nanofiber membrane capable of synergistically releasing vitamin A and vitamin E. The preparation method comprises the steps of (1) adding vitamin E polyethylene glycol succinate to a gelatin solution, (2) adding vitamin A palmitate to the gelatin solution in which the vitamin E polyethylene glycol succinate is dissolved, dissolving to obtain an electrospinning liquid, and performing electrospinning to obtain the gelatin nanofiber membrane carrying the vitamin A and the vitamin E, and (3) cross-linking the spun gelatin nanofiber membrane by use of steam, thereby obtaining the gelatin nanofiber membrane capable of synergistically releasing the vitamin A and the vitamin E. The preparation method is simple, low in cost, and relatively high in efficiency, and has favorable application prospect in the aspects of functional dressings and anti-oxidation skin care products; the obtained nanofiber membrane is capable of realizing continuous and synergistic release of the vitamin A and the vitamin E needed by wound healing or skin protection, and can be applied to the healing performance analysis of the anti-oxidation skin care products or the wound surface dressing materials.
Description
Technical field
The invention belongs to the preparation field of nano fibrous membrane, particularly the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A and vitamin E.
Background technology
Wound can be defined as by machinery, electricity, heat, chemistry etc. factor cause or become morphogenetic skin defect by a kind of medical science or physiology.If wound deal with improperly may suppurative infection, prolongedly not heal, even because of threat to life such as concurrent general infection, emphysematous gangrene, lockjaw.Therefore, for wound, strictly conscientious process must be carried out.And select the correct beyond doubt process wound of correct dressing and the key of accelerating wound healing.Along with going deep into researching wound healing, people recognize and use the object of dressing far from order to flap coverage, dressing also must can help wound healing, and simultaneously also due to materialogy and technological progress, dressing there occurs breakthrough change in the nearly more than ten years.
New pattern compress, this type of dressing because of can with wound all close adhesion, prevent surface of a wound drying to be also called seal dressing, a kind of dressing maintaining wound moist environment, its absorbability is relevant with its structure.It can act on mutually with the medicament of topical application and body endogenous molecule, promotes wound healing.The advantage of this type of dressing is that it can when facing different physiological environment, and the chemical environment of amendment wound area is with accelerating wound healing, and it is made up of biopolymer usually, and comprise active additive, and as antiseptic, antibiotic, all kinds of growth factor, nano-metal particle etc.The form of current modal several new pattern compress comprises film, foam, aquogel and hydrophilic fibers etc., and wherein nanofiber dressing receives concern especially.Nano-fiber material has the incomparable advantage of a lot of other materials, such as high specific area.And electrostatic spinning, as method comparatively general in nano-fiber material, naturally also become the popular method of the novel wound dressing of preparation.The nano-fiber material prepared by electrostatic spinning has the characteristic of high hole and high-specific surface area, this feature be particularly suitable for wound exudate excretion and allow oxygen through.In addition, some have and promote that the medicine of wound reparation or protein substance can add in nanofiber by electrostatic spinning technique, realize dissimilar release.This feature is that the new pattern compress of design functionality provides the foundation.
Vitamin is humans and animals is the class trace organic substance maintaining normal physiological function and must obtain from food, also plays an important role in wound healing.Wherein vitamin A has positive role in the inflammatory phase of wound healing.It can promote that epithelial cell breaks up in formation collagen process.It is by improving inflammatory reaction to lysosome membrane effect, increase the inflammatory cells such as monocyte, macrophage and the lymphocyte entering the surface of a wound, and regulate collagenase activity, contribute to collage synthesis maturation, epithelium regeneration and vascularization, also can improve the immunological function repression caused by operation wound.Therefore replenishing vitamins A can improve wound healing.According to about report, vitamin E proves that it has anti-oxidant and effect that is anti-inflammatory, can be used as the antioxidant of the materials such as vitamin A.The oxygen free radicals that its anti-oxidation characteristics also can protect wound to discharge from granulocyte; but delayed union (vitamin A can suppress this effect) significantly time heavy dose of; therefore vitamin E can be used for revising scar formation, its effect is also for the design of functional dressing provides new thinking.
Summary of the invention
Technical problem to be solved by this invention is to provide the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A and vitamin E, the method is simple, novel, low cost and efficiency is higher, has a good application prospect in functional dressing and anti-oxidant skin care products.
The preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A of the present invention and vitamin E, comprising:
(1) in gelatin solution, vitamin E polyethylene glycol succinic acid ester is added; Wherein, the mass ratio of gelatin copolymer and vitamin E polyethylene glycol succinic acid ester is 2-10:1;
(2) in the gelatin solution dissolving vitamin E polyethylene glycol succinic acid ester, Retinol Palmitate is added, dissolve, obtain electrospinning liquid, carry out electrostatic spinning, must be loaded with the glutin nano fabric film of vitamin A and vitamin E, wherein the mass ratio of gelatin and Retinol Palmitate is 20-100:1;
(3) the nano fibrous membrane vapor crosslinking will spun in step (2), must work in coordination with the glutin nano fabric film of release vitamin A and vitamin E.
The solvent of gelatin solution is glacial acetic acid or glacial acetic acid and distilled water volume ratio in described step (1) is the mixed solvent of 4:1.
In described step (1), the concentration of gelatin solution is 10-15% grams per milliliter.
In described step (1), the mass ratio of gelatin and vitamin E polyethylene glycol succinic acid ester is 2:1,5:1 or 10:1.
In described step (2), the mass ratio of gelatin and Retinol Palmitate is 20:1,50:1 or 100:1.
In described step (2), the technological parameter of electrostatic spinning is: voltage 10-16 kilovolt, syringe fltting speed is 0.2-0.8 ml/hour, and receiving range is 120-200 millimeter, uses No. 9 syringe needles.
In described step (3), the crosslinking agent of vapor crosslinking is the glutaraldehyde solution of 25%.
In described step (3), the vapor crosslinking time is 12-24 hour.
The consumption of 25% described glutaraldehyde cross-linking is 10-20 milliliter.
In described step (3), the area of glutin nano fabric film is 10cm*10cm.
beneficial effect
(1) simple, the low cost of the inventive method and efficiency is higher, has a good application prospect in functional dressing and anti-oxidant skin care products;
(2) the gelatin Electrospun carrying vitamin A and vitamin E of gained of the present invention prepares nano fibrous membrane; what can realize vitamin A needed for wound healing or skin sparing and vitamin E continues collaborative release, can be applied to the callus performance evaluation of anti-oxidant skin care products or Wound dressing material.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph of the glutin nano fabric film (mass ratio of gelatin and Retinol Palmitate and vitamin E polyethylene glycol succinic acid ester is respectively 100:1 and 10:1) of worked in coordination with release vitamin A and the vitamin E obtained in embodiment 1;
Fig. 2 is the transmission electron microscope photo of the glutin nano fabric film (mass ratio of gelatin and Retinol Palmitate and vitamin E polyethylene glycol succinic acid ester is respectively 50:1 and 5:1) of worked in coordination with release vitamin A and the vitamin E obtained in embodiment 3;
Fig. 3 is vitamin A in phosphate buffer (pH 7.4) of the glutin nano fabric film of worked in coordination with release vitamin A and the vitamin E obtained in embodiment 2 and E In Vitro Dissolution curve.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
Take 0.7 gram of gelatin powder with electronic analytical balance to be dissolved in 5 milliliters of glacial acetic acid, obtain the gelatin solution that concentration is 14% (grams per milliliter), 0.07 gram of vitamin E polyethylene glycol succinic acid ester is taken with electronic analytical balance, be dissolved in the gelatin solution of 14% (grams per milliliter), normal temperature lower magnetic force is stirred to and dissolves completely, Retinol Palmitate 0.0007 gram is added in solution after dissolving, it is electrospinning liquid that normal temperature lower magnetic force is stirred to dissolving (in solution, the mass ratio of gelatin and Retinol Palmitate and vitamin E polyethylene glycol succinic acid ester is respectively 100:1 and 10:1) completely, voltage is 14 kilovolts, syringe fltting speed is 0.8 ml/hour, receiving range is 150 millimeters, select No. 9 syringe needles, aluminium-foil paper receives, obtain the gelatin Electrospun nano-fibers film carrying vitamin A and vitamin E that size is 10 centimetres x10 centimetre.With 10 milliliter of 25% glutaraldehyde cross-linking after 24 hours, obtain the gelatin Electrospun nano-fibers film can working in coordination with release vitamin A and vitamin E.
Embodiment 2
Take 0.6 gram of gelatin powder with electronic analytical balance to be dissolved in the mixed solvent (glacial acetic acid and distilled water volume ratio are 4:1) of 6 milliliters of glacial acetic acid and water, obtain the gelatin solution that concentration is 10% (grams per milliliter), 0.3 gram of vitamin E polyethylene glycol succinic acid ester is taken with electronic analytical balance, be dissolved in the gelatin solution of 10% (grams per milliliter), normal temperature lower magnetic force is stirred to and dissolves completely, Retinol Palmitate 0.03 gram is added in solution after dissolving, it is electrospinning liquid that normal temperature lower magnetic force is stirred to dissolving (in solution, the mass ratio of gelatin and Retinol Palmitate and vitamin E polyethylene glycol succinic acid ester is respectively 20:1 and 2:1) completely, voltage is 16 kilovolts, syringe fltting speed is 0.2 ml/hour, receiving range is 120 millimeters, select No. 9 syringe needles, aluminium-foil paper receives, obtain the gelatin Electrospun nano-fibers film carrying vitamin A and vitamin E that size is 10 centimetres x10 centimetre.With 20 milliliter of 25% glutaraldehyde cross-linking after 21 hours, obtain the gelatin Electrospun nano-fibers film can working in coordination with release vitamin A and vitamin E.
Embodiment 3
Take 0.8 gram of gelatin powder with electronic analytical balance to be dissolved in 7 milliliters of glacial acetic acid, obtain the gelatin solution that concentration is 11.4% (grams per milliliter), 0.16 gram of vitamin E polyethylene glycol succinic acid ester is taken with electronic analytical balance, be dissolved in the gelatin solution of 11.4% (grams per milliliter), normal temperature lower magnetic force is stirred to and dissolves completely, Retinol Palmitate 0.016 gram is added in solution after dissolving, it is electrospinning liquid that normal temperature lower magnetic force is stirred to dissolving (in solution, the mass ratio of gelatin and Retinol Palmitate and vitamin E polyethylene glycol succinic acid ester is respectively 50:1 and 5:1) completely, voltage is 10 kilovolts, syringe fltting speed is 0.5 ml/hour, receiving range is 200 millimeters, select No. 9 syringe needles, aluminium-foil paper receives, obtain the gelatin Electrospun nano-fibers film carrying vitamin A and vitamin E that size is 10 centimetres x10 centimetre.With 15 milliliter of 25% glutaraldehyde cross-linking after 12 hours, obtain the gelatin Electrospun nano-fibers film can working in coordination with release vitamin A and vitamin E.
Claims (9)
1. a preparation method for the glutin nano fabric film of collaborative release vitamin A and vitamin E, comprising:
(1) in gelatin solution, vitamin E polyethylene glycol succinic acid ester is added; Wherein, the mass ratio of gelatin and vitamin E polyethylene glycol succinic acid ester is 2-10:1;
(2) in the gelatin solution dissolving vitamin E polyethylene glycol succinic acid ester, add Retinol Palmitate, dissolve, obtain electrospinning liquid, carry out electrostatic spinning, the glutin nano fabric film of vitamin A and vitamin E must be loaded with; Wherein the mass ratio of gelatin and Retinol Palmitate is 20-100:1;
(3) the nano fibrous membrane vapor crosslinking will spun in step (2), must work in coordination with the glutin nano fabric film of release vitamin A and vitamin E.
2. the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A according to claim 1 and vitamin E, it is characterized in that, the solvent of gelatin solution is glacial acetic acid or glacial acetic acid and distilled water volume ratio in described step (1) is the mixed solvent of 4:1.
3. the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A according to claim 1 and vitamin E, is characterized in that, in described step (1), the concentration of gelatin solution is 10-15% grams per milliliter.
4. the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A according to claim 1 and vitamin E, it is characterized in that, in described step (1), the mass ratio of gelatin and vitamin E polyethylene glycol succinic acid ester is 2:1,5:1 or 10:1.
5. the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A according to claim 1 and vitamin E, is characterized in that, in described step (2), the mass ratio of gelatin and Retinol Palmitate is 20:1,50:1 or 100:1.
6. the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A according to claim 1 and vitamin E, it is characterized in that, in described step (2), the technological parameter of electrostatic spinning is: voltage 10-16 kilovolt, syringe fltting speed is 0.2-0.8 ml/hour, receiving range is 120-200 millimeter, uses No. 9 syringe needles.
7. the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A according to claim 1 and vitamin E, is characterized in that, in described step (3), the crosslinking agent of vapor crosslinking is the glutaraldehyde solution of 25%.
8. the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A according to claim 1 and vitamin E, is characterized in that, in described step (3), the vapor crosslinking time is 12-24 hour.
9. the preparation method of the glutin nano fabric film of a kind of collaborative release vitamin A according to claim 1 and vitamin E, is characterized in that, in described step (3), the area of glutin nano fabric film is 10cm*10cm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105797195A (en) * | 2016-05-26 | 2016-07-27 | 东华大学 | Preparation method of nanofiber wound dressing slowly releasing vitamin E polyethylene glycol succinate |
| CN106616022A (en) * | 2016-12-05 | 2017-05-10 | 陈庆功 | Carassius auratus feed |
| CN113952114A (en) * | 2021-09-24 | 2022-01-21 | 北京科技大学 | Multilayered nanofiber dressing with patterned releasable gas and method of making same |
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| KR20110110482A (en) * | 2010-04-01 | 2011-10-07 | 중앙대학교 산학협력단 | Hyaluronic acid nanofiber comprised vitamin |
| CN102560887A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof |
| CN104047115A (en) * | 2014-07-04 | 2014-09-17 | 东华大学 | Electrostatic spinning preparation method of collagen nanofiber membrane capable of releasing vitamin E |
| CN104060401A (en) * | 2014-07-04 | 2014-09-24 | 东华大学 | Electrostatic spinning preparing method of gelatin nanofiber membrane capable of releasing vitamin A |
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2015
- 2015-05-07 CN CN201510227786.5A patent/CN104846555A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110110482A (en) * | 2010-04-01 | 2011-10-07 | 중앙대학교 산학협력단 | Hyaluronic acid nanofiber comprised vitamin |
| CN102560887A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof |
| CN104047115A (en) * | 2014-07-04 | 2014-09-17 | 东华大学 | Electrostatic spinning preparation method of collagen nanofiber membrane capable of releasing vitamin E |
| CN104060401A (en) * | 2014-07-04 | 2014-09-24 | 东华大学 | Electrostatic spinning preparing method of gelatin nanofiber membrane capable of releasing vitamin A |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105797195A (en) * | 2016-05-26 | 2016-07-27 | 东华大学 | Preparation method of nanofiber wound dressing slowly releasing vitamin E polyethylene glycol succinate |
| CN106616022A (en) * | 2016-12-05 | 2017-05-10 | 陈庆功 | Carassius auratus feed |
| CN113952114A (en) * | 2021-09-24 | 2022-01-21 | 北京科技大学 | Multilayered nanofiber dressing with patterned releasable gas and method of making same |
| CN113952114B (en) * | 2021-09-24 | 2022-09-09 | 北京科技大学 | Multilayered nanofiber dressing with patterned releasable gas and method of making same |
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Application publication date: 20150819 |