CN113952114A - Multilayered nanofiber dressing with patterned releasable gas and method of making same - Google Patents
Multilayered nanofiber dressing with patterned releasable gas and method of making same Download PDFInfo
- Publication number
- CN113952114A CN113952114A CN202111122402.5A CN202111122402A CN113952114A CN 113952114 A CN113952114 A CN 113952114A CN 202111122402 A CN202111122402 A CN 202111122402A CN 113952114 A CN113952114 A CN 113952114A
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- Prior art keywords
- nanofiber
- sponge
- patterned
- dressing
- freezing
- Prior art date
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- Granted
Links
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Images
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- A61H2033/146—Devices for gas baths with ozone, hydrogen, or the like with nitrogen
Abstract
The invention relates to the technical field of biological materials and interfacial chemistry, in particular to a multi-layer nanofiber dressing with patterned releasable gas and a preparation method thereof. The multilayer nanofiber wound dressing comprises one or more layers of nanofiber sponges with controllable pore morphology and one or more layers of nanofiber membranes with patterning; the patterning refers to imprinting a polygonal pattern on one or more layers of nanofiber membranes, and obtaining a stable multilayer nanofiber wound dressing by ultraviolet induced crosslinking. The functions of the nano-fibers in different layers are different, and the whole dressing can simultaneously have the functions of stopping bleeding, resisting bacteria and oxidation and promoting wound healing by combining gas treatment, and has good elastic performance.
Description
Technical Field
The invention relates to the technical field of biological materials and interfacial chemistry, in particular to a multi-layer nanofiber dressing with patterned releasable gas and a preparation method thereof.
Background
Healing of chronic wounds places a significant burden on the healthcare system. The healing process consists of several overlapping stages, including hemostasis, inflammation, proliferation and remodeling. Prolonged inflammation is considered to be an important factor in delayed healing of chronic wounds. In order to pass the inflammatory phase, the bacterial load, tissue necrosis and moisture balance of chronic wounds must be adequately considered.
The large volume of exudate from chronic wounds contains high levels of inflammatory mediators and proteases, which make treatment difficult, as well as inactivate and dilute bioactive therapeutic molecules and destroy the extracellular matrix and wound bed. Exudate management of conventional dressings relies on the absorption and retention of exudate by hydrophilic materials, such as hydrogels, hydrophilic nanofibers, cellulose sponges, and the like. However, when the hydrophilic conventional dressing is in close contact with the wound for a long time, excessive exudate remaining in the dressing may be reversely exuded to the periphery of the wound, so that the wound is excessively hydrated to damage tissues around the wound, thereby delaying healing. To overcome this dilemma, surgical debridement must be used, which may lead to secondary trauma and severe pain. Therefore, there is a great need for wound dressings with efficient exudate management capabilities to establish an improved wound bed environment. In addition, the use of excess exudate to trigger the release of therapeutic gas molecules and to coordinate the delivery of multifunctional bioactive molecules is expected to promote wound healing while balancing the wound microenvironment.
Disclosure of Invention
In view of the above technical problems, a first object of the present invention is to provide a multilayered nanofiber wound dressing with surface patterning and gas release capability; the second purpose of the invention is to provide the preparation method of the multilayer nanofiber wound dressing, the method is simple and easy to operate, the shaping is convenient, the prepared wound dressing has a multilayer structure, the physical properties or chemical properties of nanofiber materials of different layers are different, the impregnation phenomenon of a wound bed body fluid can be greatly reduced, and in addition, the anti-inflammatory and antibacterial treatment can be performed according to the wound condition.
The invention is realized by the following technical scheme:
a method of making a multilayered nanofiber dressing having a patterned releasable gas, the method comprising:
(1) preparing the nanofiber sponge with a three-dimensional hierarchical pore structure and gas release triggering capacity:
mixing at least two of chitosan, polyethylene glycol, alginate, silk fibroin, sodium hyaluronate or collagen serving as raw materials with zeolite, and performing electrostatic spinning to obtain a nanofiber membrane;
homogenizing and emulsifying the nanofiber membrane to obtain dispersed nanofibers, freezing the dispersed nanofibers for a certain time according to a certain freezing speed and different dimensionality freezing directions, and then carrying out vacuum drying to obtain a nanofiber sponge with a pore morphology controllable three-dimensional hierarchical pore structure and triggering gas release capacity; the nanofiber sponge is placed in a pressure container, and is exposed to dry nitric oxide under certain pressure, the nitric oxide is sealed in the nanofiber sponge and can escape in a wet environment, the nitric oxide cannot escape in the subsequent preparation process, and the prepared dressing is placed in a drying dish in the subsequent process, so that the gas can be ensured not to escape in the nanofiber sponge.
In the steps, the difference of the freezing time, the freezing speed and the direction (including unidirectional or multidirectional gradient) can cause the difference of the appearance of the ice crystals, the hierarchical pore nanofiber sponge with the ice crystal appearance is obtained by controlling the freezing time, the freezing speed and rearranging according to the appearance of the ice crystals, and the hierarchical pore nanofiber sponge with the pore appearance controllable and the three-dimensional hierarchical pore structure with the trigger gas release capacity is obtained after vacuum drying.
Preferably, uniformly mixing the polyvinyl alcohol solution and the chitosan solution to obtain a mixed solution, and then performing electrostatic spinning to obtain a nanofiber membrane; wherein, chitosan is dissolved in acetic acid solution to obtain the chitosan solution, the mass concentration of the chitosan in the chitosan solution is 3-5%, and the viscosity of the chitosan is 300-400 mPa.s; dissolving polyvinyl alcohol in water to obtain a polyvinyl alcohol solution with the mass concentration of 8-10%; the weight average molecular weight of the polyvinyl alcohol is 18-21 ten thousand; mixing chitosan and polyethylene glycol serving as raw materials with zeolite to obtain a mixed solution, and performing electrostatic spinning to obtain a nanofiber membrane; the mixed solution comprises 70-80% of polyvinyl alcohol solution, 20-30% of chitosan solution and 1-3% of zeolite by mass percent;
the freezing time of the nano-fiber membrane after homogenizing and emulsifying is 2-5 hours, the freezing speed is-20 ℃ per hour, and the direction is unidirectional or multidirectional gradient.
(2) Preparing a nanofiber membrane with patterning on the nanofiber sponge: dissolving a drug-loaded polymer and a photoinitiator to obtain an electrostatic spinning solution, wherein the drug-loaded polymer has a hydrophilicity different from that of a polymer used for preparing the nanofiber sponge; taking the nanofiber sponge with the hierarchical pore structure as a pattern template, and performing electrostatic spinning again above the nanofiber sponge to obtain a nanofiber membrane with polygonal patterns engraved;
(3) after the ultraviolet radiation with the wavelength of 254nm-365nm is carried out for 1-8 hours, the nanofiber membrane and the nanofiber sponge printed with polygonal patterns are crosslinked to form the surface-patterned multilayer nanofiber wound dressing with a stable structure.
Further, in the step (1), the dispersed nano-fibers are frozen according to the conditions that the freezing speed is-5 to-50 ℃/min, the freezing direction can be unidirectional or multidirectional, and the freezing time is 6 to 48 hours;
wherein, the control of the freezing direction is as follows: and pouring the homogenized and emulsified nanofiber mixture into a freeze casting mold, wherein one or more cooling elements are respectively arranged on the opposite side or the periphery of the mold, and the cooling gradient is controlled by combining a heater and a thermocouple device to realize unidirectional freezing of a sample from one side to the opposite side or multidirectional dimensional freezing from the periphery to the inside.
Further, in the step (1), the prepared nanofiber sponge has high elasticity, and can be recovered after being compressed to 70% of the volume of the non-compressed state.
Further, in the step (1), the diameter of the nanofiber in the nanofiber sponge is 512nm-879 nm.
Further, in the step (1), the specific technical parameters of the electrostatic spinning in the step (1) are as follows: the voltage is 14-28kv, the distance from the needle to the receiving device is 8-16cm, and the size of the zeolite is 50-200 nmm; the nanofiber sponge is exposed to dry nitric oxide for 1-10 hours at 4-10 atmospheres.
Further, in the step (1), the nanofiber membrane is cut into squares, immersed in an anhydrous tert-butyl alcohol solution, and then homogenized and emulsified to form dispersed nanofibers;
the mass of the nanofiber membrane is 1-50g, and the volume of the anhydrous tertiary butanol solution is 10-1000 ml. Preferably, the nanofiber membrane is cut into 1 x 1cm squares.
Further, in the step (2), the polymer used comprises any one or more of polydimethylsiloxane, polyurethane and latex; the load medicine comprises one or more of antioxidant and antibacterial medicines, and the antioxidant and antibacterial medicines comprise one or more of curcumin, thymol, gentamicin sulfate, ampicillin and amoxicillin; the photoinitiator is 2-hydroxy-4' - (2-hydroxyethoxy) -2-methyl propiophenone or Irgacure 2959;
the polymer comprises 5 wt.% to 20 wt.% of the mass of the electrospinning solution, and the photoinitiator is 0.1 wt.% to 1 wt.% of the mass of the polymer.
Further, in the step (2), the specific technical parameters of electrostatic spinning are as follows: the voltage is 20-28kv, and the distance between the needle and the receiving device is 5-13 cm.
A multilayered nanofiber dressing having a patterned releasable gas, the multilayered nanofiber wound dressing comprising one or more layers of zeolite-loaded nanofiber sponge having a three-dimensional hierarchical pore structure with triggered gas release capabilities and one or more layers of patterned nanofiber membrane; the patterning is to print one or more layers of nanofiber membranes with patterns on a nanofiber sponge with a customized porous structure obtained in a specific freezing mode as a pattern template;
the pattern on the nanofiber membrane increases the physical entanglement between the nanofiber membrane and the nanofiber sponge to reduce wound maceration.
The physical and chemical properties of nanofiber sponges and nanofiber membranes are different; the difference of the physical properties lies in the difference of hydrophilicity, and based on the difference of the hydrophilicity, the exudation liquid can be actively discharged from a wound part and absorbed by the nanofiber sponge, so that the release of nitric oxide therapeutic gas molecules is triggered; the difference in chemical properties includes difference in loaded drug or difference in chemical properties of the fiber raw material itself. The loaded medicine comprises one or more of curcumin, thymol or antioxidant or antibacterial medicine; the antioxidant and antibacterial drugs comprise gentamicin sulfate, ampicillin and amoxicillin.
Furthermore, the pore size of the nanofiber sponge is 10-80um, and the pore appearance is any one or more of honeycomb, dendritic, layered and columnar.
The invention has the beneficial technical effects that:
the present invention provides a surface patterned multilayered nanofiber wound dressing with gas release capability that has effective antimicrobial, antioxidant and exudate control properties. Under the condition of no external driving force, the micropatterned nanofiber aerogel can independently transfer liquid drops in an intelligent and unidirectional mode, has faster liquid transfer capacity, and triggers the release of nitric oxide therapeutic gas while transferring liquid (water has stronger affinity with zeolite, and when the water is in contact with the nitric oxide-loaded zeolite, the water can compete for zeolite pores which originally adsorb nitric oxide, so that the release of nitric oxide is triggered). The rapid one-way imbibition composite structure can also prevent the absorbed exudate from impregnating the wound again, and is beneficial to wound recovery.
Freezing in different directions and speeds can obtain the nanofiber sponge with honeycomb, dendritic, layered and columnar structures, and the patterned nanofibers are stably combined on the surface of the nanofiber sponge by utilizing photo-crosslinking. The multilayer nanofiber wound dressing provided by the invention overcomes the close packing of the traditional nanofiber in the preparation process, and the nanofiber sponge with the customizable three-dimensional hierarchical pore structure has enhanced mechanical properties and liquid absorption capacity, and simultaneously retains the inherent soft texture and extracellular matrix-like structure of the nanofiber.
The surface-patterned multilayer nanofiber wound dressing with gas release capacity provided by the invention can achieve a synergistic treatment effect by combining with bioactive molecules with anti-inflammatory, antioxidant and antibacterial activities, and can accelerate the promotion of wound healing in the inflammatory phase of chronic wounds.
Drawings
Fig. 1 is a schematic structural diagram of a multi-layered nanofiber dressing having a patterned releasable gas in an embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
On the contrary, the invention is intended to cover alternatives, modifications, equivalents and alternatives which may be included within the spirit and scope of the invention as defined by the appended claims. Furthermore, in the following detailed description of the present invention, certain specific details are set forth in order to provide a better understanding of the present invention. It will be apparent to one skilled in the art that the present invention may be practiced without these specific details.
Example 1
This example provides a method of making a multilayered nanofiber dressing with a patterned releasable gas:
(1) preparing the nanofiber sponge with a three-dimensional hierarchical pore structure and gas release triggering capacity:
(a) 0.2g of quaternary ammonium salt chitosan with the viscosity of 400mPa.s is added into 10mL of water for dissolution, and 1g of polyvinyl alcohol with the weight-average molecular weight of 1 ten thousand is dissolved into 10mL of water for dissolution by stirring.
(b) The quaternary ammonium salt chitosan solution and the polyvinyl alcohol solution are mixed according to the weight ratio of 3: 7, adding 1% of zeolite after mixing, stirring uniformly, injecting into a syringe, adjusting the propelling speed of the syringe to be 0.3mL/h, adjusting the distance between a needle and a receiving device to be 13cm, and adjusting the voltage between the needle and the receiving device to be 20KV, and electro-spinning for 3 hours to obtain 0.1g of nano-fiber membrane with a planar structure.
(c) Cutting the accumulated 0.5g of nanofiber membrane with a planar structure into 1cm multiplied by 1cm, placing the cut nanofiber membrane into a beaker filled with tert-butyl alcohol, placing the beaker into a homogeneous emulsifying instrument, and emulsifying for 10 minutes at 10000 rpm.
(d) And (3) putting the emulsified dispersed nanofiber solution into a square mould, freezing for 8 hours in a freezing and casting device with the lower temperature, the left temperature and the right temperature being 0 ℃ and the upper temperature being minus 80 ℃, taking out and putting into a freezing and vacuum drying machine for drying for 12 hours to obtain the nanofiber sponge with the thickness of 10mm and the fiber diameter of 634nm and in a three-dimensional multistage pore structure, and exposing the nanofiber sponge to dry nitric oxide for 8 hours under 4 atmospheric pressures.
(2) Preparation of nanofiber membranes with patterning on nanofiber sponges:
dissolving 0.8g of polylactic acid with the weight-average molecular weight of 35 ten thousand and 0.001g of 2-hydroxy-4' - (2-hydroxyethoxy) -2-methyl propiophenone in 10mL of hexafluoroisopropanol, and stirring for dissolution;
injecting polylactic acid solution into an injector, adjusting the propelling speed of the injector to be 0.2mL/h, adjusting the distance between a needle head and the nanofiber sponge with the three-dimensional hierarchical pore structure to be 8cm, and carrying out electrospinning for 20 minutes under the condition that the voltage between the needle head and the nanofiber sponge is 25 KV;
(3) upon irradiation with 365nm uv light for 1 hour, crosslinking occurred, forming a surface patterned multilayered nanofiber wound dressing with a stable structure (as shown in fig. 1).
Example 2
This example provides a method of making a multilayered nanofiber dressing with a patterned releasable gas:
(1) preparing the nanofiber sponge with a three-dimensional hierarchical pore structure and gas release triggering capacity:
(a) 0.2g of quaternary ammonium salt chitosan with the viscosity of 300mPa.s is added into 10mL of water for dissolution, and 1g of polyvinyl alcohol with the weight-average molecular weight of 8000 is dissolved in 10mL of water for dissolution by stirring.
(b) The quaternary ammonium salt chitosan solution and the polyvinyl alcohol solution are mixed according to the weight ratio of 2: 8, adding 2% of zeolite after mixing, stirring uniformly, injecting into a syringe, adjusting the propelling speed of the syringe to be 0.3mL/h, adjusting the distance between a needle and a receiving device to be 13cm, and adjusting the voltage between the needle and the receiving device to be 22KV, and electro-spinning for 3 hours to obtain 0.1g of nano-fiber membrane with a planar structure.
(c) The cumulative amount of 0.5g of the nanofiber membrane having a planar structure was cut into 1cm × 1cm and placed in a beaker containing t-butanol, and the beaker was placed in a homogeneous emulsifier and emulsified at 13000rpm for 10 minutes.
(d) And (3) putting the emulsified dispersed nanofiber solution into a square mould, freezing for 8 hours in a freezing and casting device with the temperature of-20 ℃ above, below, left and right, taking out, putting into a freezing and vacuum drying machine, and drying for 12 hours to obtain the nanofiber sponge with the thickness of 10mm and the fiber diameter of 685nm, wherein the nanofiber sponge is exposed to dry nitric oxide for 9 hours under the pressure of 5 atmospheres.
(2) Preparation of nanofiber membranes with patterning on nanofiber sponges:
0.8g of polylactic acid with a weight average molecular weight of 35 ten thousand, 0.01g of gentamicin sulfate and 0.002g of 2-hydroxy-4' - (2-hydroxyethoxy) -2-methylpropiophenone were dissolved in 10mL of hexafluoroisopropanol and stirred.
Injecting the mixed solution into an injector, adjusting the propelling speed of the injector to be 0.2mL/h, adjusting the distance between a needle head and the nanofiber sponge with the three-dimensional hierarchical pore structure to be 8cm, and carrying out electrospinning for 20 minutes under the condition that the voltage between the needle head and the nanofiber sponge is 27 KV;
(3) irradiating for 7 hours under 254nm ultraviolet light to obtain the patterned multilayer nanofiber wound dressing.
Example 3
This example provides a method of making a multilayered nanofiber dressing with a patterned releasable gas:
(1) preparing a nanofiber sponge with a three-dimensional hierarchical pore structure:
(a) 0.2g of chitosan quaternary ammonium salt with the viscosity of 1000mPa.s is added into 10mL of water for dissolution, and 1g of polyepoxy hexane with the weight-average molecular weight of 8000 is dissolved in 10mL of water for dissolution by stirring.
(b) The quaternary ammonium salt chitosan solution and the poly (hexamethylene oxide) solution are mixed according to the weight ratio of 2: 8, adding 1.5 percent of zeolite after mixing, uniformly stirring, adjusting the propelling speed of the injector to be 0.3mL/h, the distance between the needle head and the receiving device to be 13cm, and the voltage between the needle head and the receiving device to be 24KV, and electro-spinning for 3 hours to obtain 0.1g of nano-fiber membrane with a planar structure.
(c) The cumulative amount of 0.8g of the nanofiber membrane having a planar structure was cut into 1cm × 1cm and placed in a beaker containing t-butanol, and the beaker was placed in a homogeneous emulsifier and emulsified at 13000rpm for 10 minutes.
(d) And (3) putting the emulsified dispersed nanofiber solution into a square mould, freezing for 8 hours in a freezing and casting device with the temperature of-80 ℃ above, below, left and right, taking out, putting into a freezing and vacuum drying machine, and drying for 12 hours to obtain the nanofiber sponge with the thickness of 10mm and the fiber diameter of 685nm, wherein the nanofiber sponge is exposed to dry nitric oxide for 4 hours under 7 atmospheric pressures.
(2) Preparing a nanofiber membrane with patterning on the nanofiber sponge:
0.8g of polylactic acid having a weight average molecular weight of 35 ten thousand, 0.02g of curcumin and 0.001g of Irgacure 2959 were dissolved in 10mL of hexafluoroisopropanol and stirred.
Injecting the mixed solution into an injector, adjusting the propelling speed of the injector to be 0.2mL/h, adjusting the distance between a needle head and the nanofiber sponge with the three-dimensional hierarchical pore structure to be 8cm, and performing electrospinning for 20 minutes under the condition that the voltage between the needle head and the nanofiber sponge is 26 KV;
(3) irradiating for 2 hours under 365nm ultraviolet light to obtain the patterned multilayer nanofiber wound dressing.
Example 4
This example provides a method of making a multilayered nanofiber dressing with a patterned releasable gas:
(1) preparing the nanofiber sponge with a three-dimensional hierarchical pore structure and gas release triggering capacity:
(a) 0.2g of chitosan quaternary ammonium salt with the viscosity of 1000mPa.s is added into 10mL of water for dissolution, and 1g of polyepoxy hexane with the weight-average molecular weight of 8000 is dissolved in 10mL of water for dissolution by stirring.
(b) The quaternary ammonium salt chitosan solution and the poly (hexamethylene oxide) solution are mixed according to the weight ratio of 2: 8, adding 1% of zeolite after mixing, stirring uniformly, injecting into a syringe, adjusting the propelling speed of the syringe to be 0.3mL/h, adjusting the distance between a needle and a receiving device to be 13cm, and adjusting the voltage between the needle and the receiving device to be 24KV, and electro-spinning for 3 hours to obtain 0.1g of nano-fiber membrane with a planar structure.
(c) The cumulative amount of 0.8g of the nanofiber membrane having a planar structure was cut into 1cm × 1cm and placed in a beaker containing t-butanol, and the beaker was placed in a homogeneous emulsifier and emulsified at 13000rpm for 10 minutes.
(d) And (3) putting the emulsified dispersed nanofiber solution into a square mould, freezing for 8 hours in a freezing and casting device with the right side at-20 ℃ and the upper side and the lower side at-60 ℃, taking out, and putting into a freezing and vacuum drying machine for drying for 12 hours to obtain a nanofiber sponge with a three-dimensional multilevel pore structure, wherein the thickness of the nanofiber sponge is 10mm, the diameter of the nanofiber is 685nm, and the nanofiber sponge is exposed to dry nitric oxide for 9 hours under 6 atmospheric pressures.
(2) Preparing a nanofiber membrane with patterning on the nanofiber sponge with a three-dimensional hierarchical pore structure:
0.8g of polylactic acid having a weight average molecular weight of 35 ten thousand, 0.01g of gallic acid and 0.003g of Irgacure 2959 were dissolved in 10mL of hexafluoroisopropanol and dissolved with stirring.
Injecting the mixed solution into an injector, adjusting the propelling speed of the injector to be 0.2mL/h, adjusting the distance between a needle head and the nanofiber sponge with the three-dimensional hierarchical pore structure to be 8cm, and electrically spinning for 20 minutes under the condition that the voltage between the needle head and the nanofiber sponge is 28 KV;
(3) irradiating for 3 hours under 365nm ultraviolet light to obtain the patterned multilayer nanofiber wound dressing.
Claims (10)
1. A method of making a multilayered nanofiber dressing having a patterned releasable gas, the method comprising:
(1) preparing the nanofiber sponge with a three-dimensional hierarchical pore structure and gas release triggering capacity:
mixing at least two of chitosan, polyethylene glycol, alginate, silk fibroin, sodium hyaluronate or collagen serving as raw materials with zeolite, and performing electrostatic spinning to obtain a nanofiber membrane;
homogenizing and emulsifying the nanofiber membrane to obtain dispersed nanofibers, freezing the dispersed nanofibers for a certain time according to a certain freezing speed and different dimensionality freezing directions, and then carrying out vacuum drying to obtain a nanofiber sponge with a pore morphology controllable three-dimensional hierarchical pore structure and triggering gas release capacity; placing the nanofiber sponge into a pressure vessel, and exposing the nanofiber sponge to dry nitric oxide under certain pressure;
(2) preparing a nanofiber membrane with patterning on the nanofiber sponge: dissolving a drug-loaded polymer and a photoinitiator to obtain an electrostatic spinning solution, wherein the drug-loaded polymer has a hydrophilicity different from that of a polymer used for preparing the nanofiber sponge; taking the nanofiber sponge with the hierarchical pore structure as a pattern template, and performing electrostatic spinning again above the nanofiber sponge to obtain a nanofiber membrane with polygonal patterns engraved;
(3) after the ultraviolet radiation with the wavelength of 254nm-365nm is carried out for 1-8 hours, the nanofiber membrane and the nanofiber sponge printed with polygonal patterns are crosslinked to form the surface-patterned multilayer nanofiber wound dressing with a stable structure.
2. The method for preparing the patterned gas-releasable multilayered nanofiber dressing according to claim 1, wherein in the step (1), the dispersed nanofibers are frozen at a freezing speed of-5 to-50 ℃/min, the freezing direction can be a unidirectional dimension or a multidirectional dimension, and the freezing time is 6 to 48 hours;
wherein, the control of the freezing direction is as follows: and pouring the homogenized and emulsified nanofiber mixture into a freeze casting mold, wherein one or more cooling elements are respectively arranged on the opposite side or the periphery of the mold, and the cooling gradient is controlled by combining a heater and a thermocouple device to realize unidirectional freezing of a sample from one side to the opposite side or multidirectional dimensional freezing from the periphery to the inside.
3. The method for preparing a multi-layered nanofiber dressing with a patterned gas-releasable function as claimed in claim 1, wherein the nanofiber sponge obtained in step (1) has high elasticity and can recover its original shape after being compressed to 70% of its volume in a non-compressed state.
4. The method of claim 1, wherein in step (1), the diameter of the nanofibers in the nanofiber sponge is 512nm to 879 nm.
5. The method for preparing the patterned gas-releasable multilayered nanofiber dressing according to claim 1, wherein the specific technical parameters of the electrospinning in the step (1) are as follows: the voltage is 14-28kv, the distance from the needle to the receiving device is 8-16cm, and the size of the zeolite is 50-200 nmm; the nanofiber sponge is exposed to dry nitric oxide for 1-10 hours at 4-10 atmospheres.
6. The method of claim 1, wherein in step (1), the nanofiber membrane is cut into square pieces, immersed in anhydrous t-butanol solution, and then homogenized and emulsified to form dispersed nanofibers.
7. The method for preparing the patterned gas-releasable multilayered nanofiber dressing of claim 1, wherein the polymer used in the step (2) comprises any one or more of polydimethylsiloxane, polyurethane and latex; the load medicine comprises one or more of antioxidant and antibacterial medicines, and the antioxidant and antibacterial medicines comprise one or more of curcumin, thymol, gentamicin sulfate, ampicillin and amoxicillin; the photoinitiator is 2-hydroxy-4' - (2-hydroxyethoxy) -2-methyl propiophenone or Irgacure 2959; the polymer comprises 5 wt.% to 20 wt.% of the mass of the electrospinning solution, and the photoinitiator is 0.1 wt.% to 1 wt.% of the mass of the polymer.
8. The method for preparing the patterned gas-releasable multilayered nanofiber dressing according to claim 1, wherein the specific technical parameters of the electrospinning in the step (2) are as follows: the voltage is 20-28kv, and the distance between the needle and the receiving device is 5-13 cm.
9. A multilayered nanofiber dressing with patterned releasable gas, characterized in that the multilayered nanofiber wound dressing comprises one or more layers of zeolite-loaded nanofiber sponge with a stereo hierarchical pore structure with triggered gas release capability and one or more layers of patterned nanofiber membrane; the patterning is to print one or more layers of nanofiber membranes with patterns on a nanofiber sponge with a customized porous structure obtained in a specific freezing mode as a pattern template;
the pattern on the nanofiber membrane increases the physical entanglement between the nanofiber membrane and the nanofiber sponge to reduce wound maceration;
the physical and chemical properties of nanofiber sponges and nanofiber membranes are different; the difference of the physical properties lies in the difference of hydrophilicity, and based on the difference of the hydrophilicity, the exudation liquid can be actively discharged from a wound part and absorbed by the nanofiber sponge, so that the release of nitric oxide therapeutic gas molecules is triggered; the difference in chemical properties includes difference in loaded drug or difference in chemical properties of the fiber raw material itself.
10. The multi-layered nanofiber dressing with a patterned releasable gas according to claim 9, wherein the nanofiber sponge has a pore size of 10-80um and a pore morphology of any one or more of honeycomb, dendritic, lamellar and columnar shape.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2709289A1 (en) * | 2007-12-12 | 2009-06-18 | 3M Innovative Properties Company | Hydrophilic gel materials and methods of making |
| CN103990175A (en) * | 2014-06-10 | 2014-08-20 | 吉林大学 | Drug controlled-release double-layer nanofiber wound dressing and preparation method thereof |
| CN104846555A (en) * | 2015-05-07 | 2015-08-19 | 东华大学 | Preparation method of gelatin nanofiber membrane capable of synergistically releasing vitamin A and vitamin E |
| CN105873621A (en) * | 2013-12-30 | 2016-08-17 | 芬欧汇川集团 | Patterned membrane |
| CN106390177A (en) * | 2016-09-28 | 2017-02-15 | 深圳先进技术研究院 | Chitosan-based multi-layer nanofiber membrane dressing as well as preparation method and application thereof |
| CN107753996A (en) * | 2017-10-19 | 2018-03-06 | 五邑大学 | A kind of moisture absorption antibacterial 3D nano-fiber medical dressings and preparation method thereof |
| EP3423011A1 (en) * | 2016-03-01 | 2019-01-09 | KCI Licensing, Inc. | Drape for use with medical therapy systems |
| CN109481730A (en) * | 2018-11-27 | 2019-03-19 | 五邑大学 | Calcium alginate/polyethylene glycol three-dimensional fiber network gel and its preparation method and application |
| CN110917386A (en) * | 2019-12-10 | 2020-03-27 | 康妍葆(北京)干细胞科技有限公司 | Stem cell sponge scaffold, preparation method and application |
| CN111529748A (en) * | 2020-04-16 | 2020-08-14 | 东华大学 | Medical composite dressing for wound repair and preparation method thereof |
-
2021
- 2021-09-24 CN CN202111122402.5A patent/CN113952114B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2709289A1 (en) * | 2007-12-12 | 2009-06-18 | 3M Innovative Properties Company | Hydrophilic gel materials and methods of making |
| CN105873621A (en) * | 2013-12-30 | 2016-08-17 | 芬欧汇川集团 | Patterned membrane |
| CN103990175A (en) * | 2014-06-10 | 2014-08-20 | 吉林大学 | Drug controlled-release double-layer nanofiber wound dressing and preparation method thereof |
| CN104846555A (en) * | 2015-05-07 | 2015-08-19 | 东华大学 | Preparation method of gelatin nanofiber membrane capable of synergistically releasing vitamin A and vitamin E |
| EP3423011A1 (en) * | 2016-03-01 | 2019-01-09 | KCI Licensing, Inc. | Drape for use with medical therapy systems |
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| CN111529748A (en) * | 2020-04-16 | 2020-08-14 | 东华大学 | Medical composite dressing for wound repair and preparation method thereof |
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| CN114618006A (en) * | 2022-04-11 | 2022-06-14 | 北京服装学院 | Intelligent wound dressing, preparation method and application thereof, and flexible sensor |
| CN114618006B (en) * | 2022-04-11 | 2022-11-25 | 北京服装学院 | Intelligent wound dressing, preparation method and application thereof, and flexible sensor |
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