CN104060401A - Electrostatic spinning preparing method of gelatin nanofiber membrane capable of releasing vitamin A - Google Patents
Electrostatic spinning preparing method of gelatin nanofiber membrane capable of releasing vitamin A Download PDFInfo
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- CN104060401A CN104060401A CN201410316896.4A CN201410316896A CN104060401A CN 104060401 A CN104060401 A CN 104060401A CN 201410316896 A CN201410316896 A CN 201410316896A CN 104060401 A CN104060401 A CN 104060401A
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Abstract
The invention relates to an electrostatic spinning preparing method of a gelatin nanofiber membrane capable of releasing vitamin A. The method comprises the steps that (1) vitamin A palmitate is added into a natural polymer gelatin solution, dissolving is carried out, electro-spinning liquid is obtained, electrostatic spinning is carried out, and the nanofiber membrane with vitamin A carried is obtained; and (2) the nanofiber membrane obtained by spinning is subjected to steam crosslinking, and then the gelatin nanofiber membrane capable of releasing vitamin A is obtained. The method is simple, efficient, novel and convenient to operate, good application prospect is achieved on functionality dressing, obtained gelatin electrospining yarn with vitamin A carried is used for preparing the nanofiber membrane, quick continuous releasing of vitamin A needed for wound healing can be achieved, and the method can be used for medicine releasing performance analysis of wound surface dressing materials.
Description
Technical field
The invention belongs to the preparation field of nano fibrous membrane, particularly a kind of electro-spinning Preparation Method of the glutin nano fabric film that discharges vitamin A.
Background technology
Wound healing refers to that body suffers External Force Acting, and skin etc. tissues occurs from the process more again after disconnected or damaged, is to comprise the regeneration of various tissues and the complex combination that granulation tissue hyperplasia, cicatricial tissue form, and shows the synergy of various processes.Treatment of wounds is correct, can make it heal rapidly; Otherwise possible suppurative infection, does not prolongedly heal, even because of threat to life such as concurrent general infection, emphysematous gangrene, lockjaw.Therefore,, for the wound of wound, must carry out strict conscientious processing.And select correct dressing correctly to process beyond doubt the key of wound accelerating wound healing.Traditional dressing comprises gauze, tulle etc., and its effect is only to cover wound.Along with going deep into researching wound healing, people recognize and use the object of dressing far from for flap coverage, dressing also must can help wound healing, and simultaneously also due to materialogy and technological progress, dressing, in the nearly more than ten years, breakthrough variation has occurred.
New pattern compress, also claims active dressing or revolutionary dressing, and its proposition originates from generation the experiment that british animal physiologist George Wen Te does with piggy.In research, he finds that the wound healing time of polyethylene film covering protection exposes wound therapy and saves 50%, thereby has established the theoretical foundation of the new pattern compress processing surface of a wound.After this, launch from all parts of the world about the research of new pattern compress.The design philosophy of new pattern compress is mainly based on following several respects:
(1) flap coverage is not only wanted in dressing, but also will initiatively participate in the main process of wound healing;
(2) comfortable, to patient's no pain, have no adverse reaction;
(3) easy to use, meet the needs of patient under various living conditions, do not increase patient itself and nursing staff's burden;
(4) cheap, can be accepted and be used by extensive patients.
Therefore, for the more preferably new pattern compress of making, the corresponding wound type of dressing, the physicochemical properties of healing cycle and dressing self all need to be considered.
The eighties in 20th century, new road has been opened up in the preparation that the birth of electrostatic spinning is new pattern compress.Its principle forms jet for utilizing extra electric field power to make polymer solution or melt overcome surface tension at spinning nozzle capillary tip.In the time that electric-field intensity is enough high, under electrostatic repulsion and capillary acting in conjunction, polymer jet is along unsettled helical trajectory bending motion.Drafted thousands of times within a few tens of milliseconds, with solvent evaporates, jet solidify to form sub-micron to nano level superfine fiber.The nano-fiber material of preparing by electrostatic spinning has the characteristic of high hole and high-specific surface area, and this feature is particularly suitable for the excretion of wound exudate and allows oxygen to see through.In addition, some medicine or protein substances with the reparation of promotion wound can add in nanofiber by electrostatic spinning technique, realize dissimilar release.This feature is that the new pattern compress of design functionality provides the foundation.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of electro-spinning Preparation Method of the glutin nano fabric film that discharges vitamin A, and this inventive method is simple, efficient, novel and easy to operate, aspect new type functional dressing, is having a good application prospect.
The electro-spinning Preparation Method of a kind of glutin nano fabric film that discharges vitamin A of the present invention, comprising:
(1) in natural polymer gelatin solution, add Retinol Palmitate, dissolve, obtain electrospinning liquid, carry out electrostatic spinning, must be loaded with the nano fibrous membrane of vitamin A; Wherein the mass ratio of gelatin copolymer and Retinol Palmitate is 20-100: 1;
(2) by spin nano fibrous membrane vapor crosslinking after, must discharge the glutin nano fabric film of vitamin A.
In described step (1), the solvent of gelatin solution is glacial acetic acid.
In described step (1), the concentration of gelatin solution is 12-15% grams per milliliter.
In described step (1), the mass ratio of gelatin copolymer and Retinol Palmitate is 20: 1,50: 1 or 100: 1.
In described step (1), electrostatic spinning process parameter is: voltage 12-16 kilovolt, and syringe fltting speed is 0.6-1.0 ml/hour, receiving range is 100-200 millimeter, uses No. 9 syringe needle.
In described step (2), vapor crosslinking crosslinking agent used is the glutaraldehyde solution of 8-15 milliliter 25%.
In described step (2), the vapor crosslinking time is 24-30 hour.
In step (2), the area of the glutin nano fabric film of preparation is 10cm*10cm.
beneficial effect
(1) the inventive method is simple, efficient, novel and easy to operate, aspect new type functional dressing, is having a good application prospect;
(2) gained of the present invention carry the glutin nano fabric film of vitamin A, can realize the quick sustained release of the required vitamin A of wound healing, can be applied to the Release Performance analysis of Wound dressing material.
Brief description of the drawings
Fig. 1 is the stereoscan photograph of crosslinked rear glutin nano fabric film (mass ratio of gelatin and Retinol Palmitate is 20: the 1) electrostatic spinning that discharges vitamin A;
Fig. 2 is the infared spectrum of crosslinked rear glutin nano fabric film (mass ratio of gelatin and Retinol Palmitate is 100: 1) the electrostatic spinning nano tunica fibrosa that discharges vitamin A;
Fig. 3 is the vitamin A In Vitro Dissolution curve that discharges the glutin nano fabric film of vitamin A.
Detailed description of the invention
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment are only not used in and limit the scope of the invention for the present invention is described.In addition should be understood that those skilled in the art can make various changes or modifications the present invention after having read the content of the present invention's instruction, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
Take 0.7 gram of gelatin powder with electronic analytical balance and be dissolved in 5 milliliters of glacial acetic acid, obtain the gelatin solution that concentration is 14% (grams per milliliter); Take 0.007 gram of Retinol Palmitate with electronic analytical balance, be dissolved in the gelatin solution of 14% (grams per milliliter), normal temperature lower magnetic force is stirred to and dissolves (in solution, the mass ratio of gelatin and Retinol Palmitate is 100: 1) completely; After dissolving, solution is electrospinning liquid, and voltage is 14 kilovolts, and syringe fltting speed is 0.8 ml/hour, receiving range is 150 millimeters, select syringe needle No. 9, aluminium-foil paper receives, and obtaining size is the gelatin electrostatic spinning nano tunica fibrosa of year vitamin A of 10 centimetres x10 centimetre.After 24 hours, obtain discharging the gelatin electrostatic spinning nano tunica fibrosa of vitamin A with 10 milliliter of 25% glutaraldehyde cross-linking.
Embodiment 2
Take 0.6 gram of gelatin powder with electronic analytical balance and be dissolved in 5 milliliters of glacial acetic acid, obtain the gelatin solution that concentration is 12% (grams per milliliter); Take 0.03 gram of Retinol Palmitate with electronic analytical balance, be dissolved in the gelatin solution of 12% (grams per milliliter), normal temperature lower magnetic force is stirred to and dissolves (in solution, the mass ratio of gelatin and Retinol Palmitate is 20: 1) completely; After dissolving, solution is electrospinning liquid, and voltage is 16 kilovolts, and syringe fltting speed is 1.0 mls/hour, receiving range is 200 millimeters, select syringe needle No. 9, aluminium-foil paper receives, and obtaining size is the gelatin electrostatic spinning nano tunica fibrosa of year vitamin A of 10 centimetres x10 centimetre.After 28 hours, obtain discharging the gelatin electrostatic spinning nano tunica fibrosa of vitamin A with 8 milliliter of 25% glutaraldehyde cross-linking.
Embodiment 3
Take 0.75 gram of gelatin powder with electronic analytical balance and be dissolved in 5 milliliters of glacial acetic acid, obtain the gelatin solution that concentration is 15% (grams per milliliter); Take 0.015 gram of Retinol Palmitate with electronic analytical balance, be dissolved in the gelatin solution of 15% (grams per milliliter), normal temperature lower magnetic force is stirred to and dissolves (in solution, the mass ratio of gelatin and Retinol Palmitate is 50: 1) completely; After dissolving, solution is electrospinning liquid, and voltage is 12 kilovolts, and syringe fltting speed is 0.6 ml/hour, receiving range is 100 millimeters, select syringe needle No. 9, aluminium-foil paper receives, and obtaining size is the gelatin electrostatic spinning nano tunica fibrosa of year vitamin A of 10 centimetres x10 centimetre.After 30 hours, obtain discharging the gelatin electrostatic spinning nano tunica fibrosa of vitamin A with 12 milliliter of 25% glutaraldehyde cross-linking.
Claims (8)
1. an electro-spinning Preparation Method that discharges the glutin nano fabric film of vitamin A, comprising:
(1) in natural polymer gelatin solution, add Retinol Palmitate, dissolve, obtain electrospinning liquid, carry out electrostatic spinning, must be loaded with the nano fibrous membrane of vitamin A; Wherein the mass ratio of gelatin copolymer and Retinol Palmitate is 20-100: 1:
(2) by spin nano fibrous membrane vapor crosslinking after, must discharge the glutin nano fabric film of vitamin A.
2. the electro-spinning Preparation Method of a kind of glutin nano fabric film that discharges vitamin A according to claim 1, is characterized in that: in described step (1), the solvent of gelatin solution is glacial acetic acid.
3. the electro-spinning Preparation Method of a kind of glutin nano fabric film that discharges vitamin A according to claim 1, is characterized in that: in described step (1), the concentration of gelatin solution is 12-15% grams per milliliter.
4. the electro-spinning Preparation Method of a kind of glutin nano fabric film that discharges vitamin A according to claim 1, is characterized in that: in described step (1), the mass ratio of gelatin copolymer and Retinol Palmitate is 20: 1,50: 1 or 100: 1.
5. the electro-spinning Preparation Method of a kind of glutin nano fabric film that discharges vitamin A according to claim 1, it is characterized in that: in described step (1), electrostatic spinning process parameter is: voltage 12-16 kilovolt, syringe fltting speed is 0.6-1.0 ml/hour, receiving range is 100-200 millimeter, uses No. 9 syringe needle.
6. the electro-spinning Preparation Method of a kind of glutin nano fabric film that discharges vitamin A according to claim 1, is characterized in that: in described step (2), vapor crosslinking crosslinking agent used is the glutaraldehyde solution of 8-15 milliliter 25%.
7. the electro-spinning Preparation Method of a kind of glutin nano fabric film that discharges vitamin A according to claim 1, is characterized in that: in described step (2), the vapor crosslinking time is 24-30 hour.
8. the electro-spinning Preparation Method of a kind of glutin nano fabric film that discharges vitamin A according to claim 1, is characterized in that: in described step (2), the area of prepared glutin nano fabric film is 10cm*10cm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410316896.4A CN104060401A (en) | 2014-07-04 | 2014-07-04 | Electrostatic spinning preparing method of gelatin nanofiber membrane capable of releasing vitamin A |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104846555A (en) * | 2015-05-07 | 2015-08-19 | 东华大学 | Preparation method of gelatin nanofiber membrane capable of synergistically releasing vitamin A and vitamin E |
| CN112409933A (en) * | 2020-10-21 | 2021-02-26 | 江苏大学 | Production method of fish-derived gelatin and nanofiber membrane |
| CN114832157A (en) * | 2022-04-24 | 2022-08-02 | 四川大学 | Collagen membrane material for promoting functional healing of full-thickness skin wound |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3143475A (en) * | 1961-01-23 | 1964-08-04 | Hoffmann La Roche | Vitamin-containing gelatin beadlets and the process of preparing them |
| JP2005290631A (en) * | 2004-04-02 | 2005-10-20 | Teijin Ltd | Fiber structure and method for producing the same |
| JP2009013523A (en) * | 2007-07-03 | 2009-01-22 | Unitika Ltd | Microfiber assembly for assisting touch reading of braille |
| CN101406711A (en) * | 2008-11-04 | 2009-04-15 | 东华大学 | Method for preparing galvanic deposit calcium phosphorus mineralized layer superfine fibre bone material |
| CN101703796A (en) * | 2009-11-27 | 2010-05-12 | 天津大学 | Nano fibre artificial vascular graft modifying internal layer and preparation method thereof |
| CN102277737A (en) * | 2011-04-27 | 2011-12-14 | 南昌大学 | Preparation method and application of polycaprolactone/natural high-molecular composite porous scaffold |
| CN102560888A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with water-soluble vitamin E and preparation method for nano-fiber film |
| CN102560887A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof |
| CN103118670A (en) * | 2010-06-01 | 2013-05-22 | 欧鲁普雷图联邦大学 | Nanoparticulate composition containing antibiotics for intramammary administration in animals |
-
2014
- 2014-07-04 CN CN201410316896.4A patent/CN104060401A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3143475A (en) * | 1961-01-23 | 1964-08-04 | Hoffmann La Roche | Vitamin-containing gelatin beadlets and the process of preparing them |
| JP2005290631A (en) * | 2004-04-02 | 2005-10-20 | Teijin Ltd | Fiber structure and method for producing the same |
| JP2009013523A (en) * | 2007-07-03 | 2009-01-22 | Unitika Ltd | Microfiber assembly for assisting touch reading of braille |
| CN101406711A (en) * | 2008-11-04 | 2009-04-15 | 东华大学 | Method for preparing galvanic deposit calcium phosphorus mineralized layer superfine fibre bone material |
| CN101703796A (en) * | 2009-11-27 | 2010-05-12 | 天津大学 | Nano fibre artificial vascular graft modifying internal layer and preparation method thereof |
| CN103118670A (en) * | 2010-06-01 | 2013-05-22 | 欧鲁普雷图联邦大学 | Nanoparticulate composition containing antibiotics for intramammary administration in animals |
| CN102277737A (en) * | 2011-04-27 | 2011-12-14 | 南昌大学 | Preparation method and application of polycaprolactone/natural high-molecular composite porous scaffold |
| CN102560888A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with water-soluble vitamin E and preparation method for nano-fiber film |
| CN102560887A (en) * | 2012-01-17 | 2012-07-11 | 东华大学 | Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 万怡灶等: "戊二醛蒸汽交联明胶材料的性能研究", 《功能高分子学报》, vol. 12, no. 4, 30 December 1999 (1999-12-30), pages 457 - 459 * |
| 王映红等: "明胶膜的制备及其交联性能的研究", 《功能高分子学报》, vol. 16, no. 1, 30 March 2003 (2003-03-30), pages 36 - 40 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104846555A (en) * | 2015-05-07 | 2015-08-19 | 东华大学 | Preparation method of gelatin nanofiber membrane capable of synergistically releasing vitamin A and vitamin E |
| CN112409933A (en) * | 2020-10-21 | 2021-02-26 | 江苏大学 | Production method of fish-derived gelatin and nanofiber membrane |
| CN114832157A (en) * | 2022-04-24 | 2022-08-02 | 四川大学 | Collagen membrane material for promoting functional healing of full-thickness skin wound |
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Application publication date: 20140924 |