CN104840503A - Composition containing epimedium herb and doxycycline and used for livestock and poultry, and preparation method thereof - Google Patents
Composition containing epimedium herb and doxycycline and used for livestock and poultry, and preparation method thereof Download PDFInfo
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- CN104840503A CN104840503A CN201510011309.5A CN201510011309A CN104840503A CN 104840503 A CN104840503 A CN 104840503A CN 201510011309 A CN201510011309 A CN 201510011309A CN 104840503 A CN104840503 A CN 104840503A
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Abstract
The invention discloses a composition containing epimedium herb and doxycycline and used for livestock and poultry, and a preparation method thereof. The composition comprises the following raw materials by weight: 5 to 200 parts of epimedium herb and 3 to 30 parts of doxycycline. The composition can rapidly overcome the defects of poor curative effect, great dosage, high residual and high cost in therapy with antibacterial drugs due to drug resistance.
Description
Technical field
The invention belongs to poultry coli-infection treatment technology field, particularly relate to a kind of poultry composition and method of making the same containing trident wind and doxycycline.
Background technology
Escherichia coli are common conditioned pathogens, also be the normal a kind of disease sent out, it can formerly have been carried out the coffin upon burial the multiple location severe infections such as chicken acute sepsis, peritonitis, hepatitis, pneumonia, enteritis, also can secondary or mixedly to send out in virosis, causes huge economic loss to aquaculture.The beta-lactam lopps antibiotic such as amoxicillin, ampicillin, ceftiofur, ceftriaxone, amoxicillin/clavulanate, ampicillin/sulbactam are the conventional important drugs of this disease of control, along with cultivation scale intensive degree is more and more large, medication is more and more spread unchecked, its Resistant strain is more and more many, and the report about Drug Resistance of E. coli is also more.Research confirms, one of main mechanism of drug resistance produces beta-lactamase, especially extended spectrumβ-lactamase (Extended-Spectrum β-lactamase, ESBLs), and this enzyme can be hydrolyzed beta-lactam nucleus more by force and rapidly.ESBLs is the main enzyme type of beta-lactamase, and it not only to cephalo three generations and aztreonam drug resistance, and is crossing drug resistant to aminoglycoside, quinolones and sulfonamides, only to carbapenems, a mould alkene class medicaments insensitive.Many documents reported colibacillary multi-drug resistant with produce ESBLs have substantial connection, both at home and abroad to comparatively pay close attention to.Inventor has carried out the detection of food animal source pathogenic bacterium ESBLs recently, extraction, enzyme press down the researchs such as enzyme protection effect to antibiotic percent hydrolysis and sulbactam, Tazobactam Sodium and Chinese medicine; show the colibacillary drug sensitive test of part ESBLs, its multidrug resistant rate is apparently higher than non-ESBLs bacterial strain.In addition, result also shows to produce ESBLs bacterial strain not only to the serious drug resistance of the third generation cephalosporins such as ceftiofur, and also to multiple antimicrobial drug drug resistance such as fluoroquinolones, aminoglycoside and sulfonamides, fosfomycin etc., in serious multidrug resistant.Inventor is separated to the escherichia coli producing ESBLs drug resistant gene in Resistant strain, and this gene strengthens the drug resistance of antibacterial.
Colibacillary drug resistance causes raiser's drug cost to increase, and treatment extends the course for the treatment of, and mortality of livestock increases, and brings larger economic loss, and increase poultry drug residue risk to production.Therefore, the escherichia coli needing a kind of drug resistance to no matter whether in actual production badly can have the medicine of better therapeutical effect, and can shorten treatment course for the treatment of, reduce mortality rate, reduce economic loss and reduce drug residue, for raiser creates higher economic benefit, also ensureing that the mankind's is healthy and safe.
Summary of the invention
Main purpose of the present invention is to provide a kind of poultry compositions containing trident wind (formal name used at school Herba Epimedii) and doxycycline, raiser's drug cost is caused to increase to solve colibacillary drug resistance, treatment extends the course for the treatment of, mortality of livestock increases, bring larger economic loss to production, and increase the technical problem of poultry drug residue risk.
The poultry compositions containing trident wind and doxycycline that the present invention proposes, comprises the raw material of following weight portion: the trident wind of 5-200 part and the doxycycline of 3-30 part.The compositions that this programme provides, can overcome large, the residual high and defect that cost is high of weak curative effect that antimicrobial drug treatment causes due to drug resistance problems, consumption quickly, avoids that simple Chinese medicine sterilizing spectrum is narrow, sterilize slow problem.
Preferably, the raw material of following weight portion is comprised: the trident wind of 10-180 part and the doxycycline of 5-30 part.
Preferably, comprise the raw material of following weight portion: the doxycycline of the trident wind of 95 parts and 5 parts, or the trident wind of 10 parts and the doxycycline of 10 parts, or the trident wind of 150 parts and the doxycycline of 12 parts, or the trident wind of 180 parts and the doxycycline of 30 parts.
Preferably, described compositions is prepared into oral agents.
Preferably, described oral agents is powder, tablet, oral liquid or granule.
The present invention also proposes a kind of preparation method containing the poultry compositions of trident wind and doxycycline, comprises the following steps:
S1, trident wind is ground into Powdered;
S2, the Powdered trident wind of step S1 gained and pulverous doxycycline are pressed trident wind 5-200 part, the weight ratio mix homogeneously of doxycycline 3-30 part, obtains described compositions.
Preferably, in step S2, by the weight ratio mix homogeneously of trident wind 10-180 part, doxycycline 5-30 part, obtain described compositions.
Preferably, in step S2, by the weight ratio of trident wind 95 parts, doxycycline 5 parts, or the weight ratio of trident wind 10 parts, doxycycline 10 parts, or the weight ratio of trident wind 150 parts, doxycycline 12 parts, or the weight ratio mix homogeneously of trident wind 180 parts, doxycycline 30 parts, obtains described compositions.
Preferably, described compositions is prepared into oral agents.
Preferably, described oral agents is powder, tablet, oral liquid or granule.
For the drug resistance problems that current poultry coli-infection exists, inventor utilizes Chinese medicine trident wind and antimicrobial drug to form compositions.Research proves, after adding trident wind in antimicrobial drug, can reverse bacterial to the drug resistance of antimicrobial drug, thus improve the therapeutic effect of antimicrobial drug.Said composition is made powder, tablet, oral liquid or granule, add in animal and fowl fodder according to the 0.1-10g principal agent/addition of 1kg feedstuff and feed, can significantly reduce drug cost and drug residue, for raiser creates higher economic benefit, also ensure that food safety simultaneously.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is described in further detail.It is emphasized that following explanation is only exemplary, instead of in order to limit the scope of the invention and apply.
One, trident wind is to the drug resistance inversion Effect study of antimicrobial drug
Antimicrobial drug itself has the colibacillary effect for the treatment of, but has stronger drug resistance owing to producing the escherichia coli of drug resistant gene, causes antimicrobial drug not show good therapeutic effect in normal using dosage situation.
1, trident wind mix with antimicrobial drug after to colibacillary drug resistance inhibition test
96 orifice plates adopt microtest tube doubling dilution, first determine antimicrobial drug to producing the minimal inhibitory concentration of e. coli strains of ESBLs drug resistant gene and trident wind to the subinhibitory concentration producing ESBLs drug resistant gene e. coli strains, the trident wind then adding subinhibitory concentration in the medium reuses microtest tube doubling dilution and cultivates the minimal inhibitory concentration measuring antimicrobial drug; Continuous passage method is utilized to carry out Secondary Culture to product ESBLs drug resistant gene e. coli strains in the culture medium being added with same subinhibitory concentration trident wind and same concentration antimicrobial drug, until the antimicrobial drug minimal inhibitory concentration observed diminishes more than 4 times compared with before going down to posterity, can be judged as that trident wind has drug resistance inversion effect to antibacterial medical instrument.Result of the test statistics is as shown in table 1:
In table 1 subinhibitory concentration (0.06g/mL) trident hearsay generation, causes the change (μ g/mL) of producing ESBLs escherichia coli Western medicine MIC.
Above result shows that trident wind has drug resistance inversion effect to product ESBLs drug resistant gene escherichia coli.
Two, the application example of the present composition
Prepare embodiment 1-embodiment 6 according to table 2 formula and following methods: after trident wind was pulverized 60 mesh sieves, based on raw material, then with pulverous antimicrobial drug by weight ratio put into blender and mix, compositions is prepared into formulation products.
The formula of table 2 embodiment 1-6 and preparation dosage form
| Numbering | Antimicrobial drug | Trident wind | Dosage form |
| Embodiment 1 | Doxycycline 3g | 200g | Tablet |
| Embodiment 2 | Doxycycline 30g | 5g | Oral liquid |
| Embodiment 3 | Doxycycline 5g | 95g | Tablet |
| Embodiment 4 | Doxycycline 10g | 10g | Powder |
| Embodiment 5 | Doxycycline 12g | 150g | Oral liquid |
| Embodiment 6 | Doxycycline 30g | 180g | Granule |
1, embodiment 1-6 above-described embodiment is obtained the efficacy experiment of composition product to chicken escherichia coil disease
Clinical symptoms: Guangxi Qinzhou chicken house, 30 Day-old Broiler Chickens, there is fragmentary death in chicken group, cuts open the indivedual chicken of inspection and occur the pathological change of bag pericardium liver, typical bacillus coli feature.
Laboratory diagnosis: get pathologic liver dye sheet, Gram’s staining, basis of microscopic observation, the gram negative bacilli of red color visible; Tentatively be judged as bacillus coli.Cultivated by escherichia coli, biochemical test is differentiated, judges that chicken infects as bacillus coli, pcr amplification, proves to produce the ESBLs drug resistant gene having SHV type.
Test therapeutic regimen: morbidity chicken group is divided into 8 groups of administrations, first group with the addition administration of embodiment 1 according to 0.1g/kg feedstuff, second group with the addition administration of embodiment 2 according to 10g/kg feedstuff, 3rd group with the addition administration of embodiment 3 according to 4g/kg feedstuff, 4th group with the addition administration of embodiment 4 according to 0.4g/kg feedstuff, 5th group with the addition administration of embodiment 5 according to 2.8g/kg feedstuff, the 6th group with the addition administration of embodiment 6 according to 1.4g/kg feedstuff; 7th group with the addition administration of doxycycline powder according to 0.2g/kg feedstuff, the 8th group with the addition administration of the thick powder of trident wind according to 5g/kg feedstuff; Above eight groups of therapeutic regimens all continue use 5 days.
Staining effect: after the chicken group medication of first group to the 6th group, sick chicken feed intake rises, and no longer occurs dead chicken, and continue use 3 days, chicken group be almost recovered; The chicken group medication of the 7th group is after 2 days, and chicken group majority is still unhealthy, still has indivedual chicken death, and continue use 3 days, chicken death phenomenon is not still effectively controlled; The chicken group medication of the 8th group is after 2 days, and chicken group does not have too large remarkable improvement, and continue use 3 days, chicken death phenomenon is not still effectively controlled.Visible, the compositions curative effect containing trident wind is better than folk prescription.
Those skilled in the art will recognize that, it is possible for making numerous accommodation to above description, so embodiment is only used to describe one or more particular implementation.
Although described and described and be counted as example embodiment of the present invention, it will be apparent to those skilled in the art that and can make various change and replacement to it, and spirit of the present invention can not have been departed from.In addition, many amendments can be made so that particular case is fitted to religious doctrine of the present invention, and central concept of the present invention described here can not be departed from.So the present invention is not limited to specific embodiment disclosed here, but the present invention also may comprise all embodiments and equivalent thereof that belong to the scope of the invention.
Claims (10)
1. contain a poultry compositions for trident wind and doxycycline, it is characterized in that, comprise the raw material of following weight portion: the trident wind of 5-200 part and the doxycycline of 3-30 part.
2. poultry compositions as claimed in claim 1, is characterized in that, comprise the raw material of following weight portion: the trident wind of 10-180 part and the doxycycline of 5-30 part.
3. poultry compositions as claimed in claim 2, it is characterized in that, comprise the raw material of following weight portion: the doxycycline of the trident wind of 95 parts and 5 parts, or the doxycycline of the trident wind of 10 parts and 10 parts, or the doxycycline of the trident wind of 150 parts and 12 parts, or the trident wind of 180 parts and the doxycycline of 30 parts.
4. the poultry compositions as described in any one of claim 1-3, is characterized in that, described compositions is prepared into oral agents.
5. poultry compositions as claimed in claim 4, it is characterized in that, described oral agents is powder, tablet, oral liquid or granule.
6. contain a preparation method for the poultry compositions of trident wind and doxycycline, it is characterized in that, comprise the following steps:
S1, trident wind is ground into Powdered;
S2, the Powdered trident wind of step S1 gained and pulverous doxycycline are pressed trident wind 5-200 part, the weight ratio mix homogeneously of doxycycline 3-30 part, obtains described compositions.
7. preparation method as claimed in claim 6, is characterized in that, in step S2, by the weight ratio mix homogeneously of trident wind 10-180 part, doxycycline 5-30 part, obtain described compositions.
8. preparation method as claimed in claim 7, it is characterized in that, in step S2, by the weight ratio of trident wind 95 parts, doxycycline 5 parts, or the weight ratio of trident wind 10 parts, doxycycline 10 parts, or the weight ratio of trident wind 150 parts, doxycycline 12 parts, or trident wind 180 parts, doxycycline 30 parts weight ratio mix homogeneously, obtain described compositions.
9. the preparation method as described in any one of claim 6-8, is characterized in that, described compositions is prepared into oral agents.
10. preparation method as claimed in claim 9, it is characterized in that, described oral agents is powder, tablet, oral liquid or granule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510011309.5A CN104840503A (en) | 2015-01-09 | 2015-01-09 | Composition containing epimedium herb and doxycycline and used for livestock and poultry, and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510011309.5A CN104840503A (en) | 2015-01-09 | 2015-01-09 | Composition containing epimedium herb and doxycycline and used for livestock and poultry, and preparation method thereof |
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| CN104840503A true CN104840503A (en) | 2015-08-19 |
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| CN201510011309.5A Pending CN104840503A (en) | 2015-01-09 | 2015-01-09 | Composition containing epimedium herb and doxycycline and used for livestock and poultry, and preparation method thereof |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104027368A (en) * | 2013-06-13 | 2014-09-10 | 广西南宁市桃源兽药厂 | Plumbago zeylanica and doxycycline containing compound composition for livestock and poultry |
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2015
- 2015-01-09 CN CN201510011309.5A patent/CN104840503A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104027368A (en) * | 2013-06-13 | 2014-09-10 | 广西南宁市桃源兽药厂 | Plumbago zeylanica and doxycycline containing compound composition for livestock and poultry |
Non-Patent Citations (3)
| Title |
|---|
| YOCO MIYASAKI等: "Screening of Herbal Extracts against Multi-Drug Resistant Acinetobacter baumannii", 《PHYTOTHERAPY RESEARCH》 * |
| 宋丽娜: "四川淫羊藿化学成分和抑菌活性研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
| 邓家刚等: "《桂药原色图鉴》", 31 October 2008, 上海科学技术出版社 * |
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Application publication date: 20150819 |