CN104839008A - Breeding method for high-quality strong gluten spring wheat - Google Patents
Breeding method for high-quality strong gluten spring wheat Download PDFInfo
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- CN104839008A CN104839008A CN201510228315.6A CN201510228315A CN104839008A CN 104839008 A CN104839008 A CN 104839008A CN 201510228315 A CN201510228315 A CN 201510228315A CN 104839008 A CN104839008 A CN 104839008A
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Abstract
The invention discloses a breeding method for high-quality strong gluten spring wheat, and belongs to the field of crop breeding. Breeding is carried out through high-yielding parents and high-quality strong gluten parents, wherein the high-yielding parents are selected from high-yield varieties which are planted in Sinkiang in a large scale and are proved by many years` practice, and the high-quality strong gluten parents are selected from germplasms which have further genetic distances with local parents, are complementary with local parent characters, and have no obvious disadvantages, and adopt a high molecular weight glutenin subunit electropherogram analysis method to carry out a glutenin subunit genetic analysis on parent materials; by adopting the breeding method, a new strong gluten, high-yielding and early-maturing spring wheat variety can be cultivated.
Description
Technical field
The present invention relates to a kind of breeding method of high-quality strong gluten spring wheat, belong to crop breeding field.
Background technology
At present, domestic have utilize breeding material to carry out glutenin subunit genetic analysis and be bred as the report that wheat product (being) plant; Also there is the report utilizing glutenin subunit analysis in conjunction with breeding methods such as 60Co gamma-ray irradiation and biochemical markers, but have no the report adopting above-mentioned integrated approach to be bred as strong muscle, high yield, precocious spring wheat variety.
Summary of the invention
(1) technical problem that will solve
For solving the problem, the present invention proposes a kind of breeding method of high-quality strong gluten spring wheat.
(2) technical scheme
The breeding method of high-quality strong gluten spring wheat of the present invention, comprises hybrid strain selection, hybrid combination preparation, cross-pollinated seed irradiation mutagenesis, progeny selection and attribute test and new lines is identified.
1, the step that hybrid strain is selected is:
A) rapid extraction of sample: choose 1 seed, grind and be placed in 1.5mL centrifuge tube, add 800 μ L sample buffer (62.5mmol/L Tris-HCl solution, pH value 6.8,10% glycerine, 2%SDS, 5% beta-mercaptoethanol) the rear cradle vibrate 30min of mixing immediately, puts into 90 DEG C of water-baths and extracts 5min, take out centrifuge tube by centrifuge tube, the centrifugal 5min of 800r/min, gets supernatant and is placed in 4 DEG C of Refrigerator stores.
B) gel preparation: adopt sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) discontinuous buffer system, configuration separation gel with 10% SDS-PAGE (T=10.0%, C=2.67%, Tris 0.375mol/L, pH8.8), the concentrated glue of configuration with 4.0% SDS-PAGE (T=4.0%, C=2.67%, Tris 0.125mol/L, pH6.8), the thick 1mm of glue, two plate, sample comb is 21 holes, each sample well point sample 8-10 μ L.
C) deposition condition: electrode buffer is 25mmol/L TrisHCl, 192mmol/L glycine, 0.1%SDS, pH8.3.Two plate 30mA current stabilization electrophoresis cross liquid.
D) dyeing-decolorzing and preservation: gel 12% ethapon fixes 20min, 0.05% coomassie brilliant blue R_250 dyeed liquid, decoloured with distilled water.
E) glutenin subunit name and scoring: by above hmw glutenin subunit Electrophoretic method, obtains that the HMW-GS of new varieties consists of 1,7+9,5+10; The HMW-GS of original kind consists of 2*, 7+9,2+12; Simultaneously again grain protein content, wet gluten content and flour farinograph are carried out to new varieties, tensilometer measured and analyzed; Toasted bread, new quality reaches the strong muscle standard of GB.
2, the step of hybrid combination preparation is: plantation parent garden (if answering interval sowing when two parent's heading period differences are larger), after female parent is eared 2 days, choose maternal standard fringe (main fringe) 5 fringes, every fringe top and bottom small ear is rejected, little Hua 20 in the middle of retaining, removed by stamen in 20 little Hua, bagging is numbered; Pollinate when male parent flowering reaches 40%-50% (every day, pollinations in 8 o'clock to 12 o'clock were in the morning better, if pollen amount can adopt greatly twist with the fingers fringe method).
3, the step of cross-pollinated seed irradiation mutagenesis is: the mutagenesis of cross-pollinated seed 60Co gamma-ray irradiation.The seed moisture content of irradiation is controlled between 14%-16%; Measure irradiation chamber dose rate be 1 gray(Gy)/minute point; Irradiation seed is evenly placed in dose rate be 1 gray(Gy)/minute point on, irradiation dose is that 80 gray(Gy)s carry out irradiation mutagenesis.
4, progeny selection with the step of attribute test is: hybridize the offspring be combined with 60Co gamma-ray irradiation, according to breeding goal through too much for individual plant, single ear selection select with south numerous added-generation (if conditions permit, south numerous added-generation; If condition does not allow, also can not south numerous added-generation), to 4-5 for time hmw glutenin subunit Electrophoretic (analytical method is the same) is carried out to the seed that excellent plant or head progeny row mix receipts, by hmw glutenin subunit Electrophoretic and in conjunction with variable rate technology, to select in HMW-GS composition containing 5+10 high quality subunit and at the outstanding plant of variable rate technology or head progeny row.
5, the step that new lines is identified is: continue to add generation and selection at the outstanding plant of variable rate technology or head progeny row containing 5+10 high quality subunit by what select, until the new lines of seed selection is entered in variety test of lines after complete stability, carry out the output of 3 years, economical character, resistance, disease resistance and quality (grain protein content, wet gluten content and flour farinograph, extensograph testing, baking bread) etc. comprehensive identification, the spring wheat variety of strong muscle, high yield can be selected.
(3) beneficial effect
Compared with prior art, it has following beneficial effect in the present invention: the breeding method of high-quality strong gluten spring wheat of the present invention, and it can cultivate the strong muscle, high yield, the precocious spring wheat variety that make new advances.
Embodiment
Below in conjunction with concrete embodiment, further restriction is done to technical scheme of the present invention, but claimed scope is not only confined to done description.
Embodiment:
By high parent's (select the local establishing in large scale in Xinjiang and high yielding variety) through facts have proved for many years and high-quality strong gluten parent (select far away with local parent's genetic distance and with local parental trait complementation, there is no the kind matter of distinct disadvantage, adopt hmw glutenin subunit Electrophoretic method, carry out the glutenin subunit genetic analysis of parent material) carry out breeding.
The breeding method of high-quality strong gluten spring wheat of the present invention:
Comprise hybrid strain selection, hybrid combination preparation, cross-pollinated seed irradiation mutagenesis, progeny selection and attribute test and new lines is identified.
1, the step that hybrid strain is selected is:
A) rapid extraction of sample: choose 1 seed, grind and be placed in 1.5mL centrifuge tube, add 800 μ L sample buffer (62.5mmol/L Tris-HCl solution, pH value 6.8,10% glycerine, 2%SDS, 5% beta-mercaptoethanol) the rear cradle vibrate 30min of mixing immediately, puts into 90 DEG C of water-baths and extracts 5min, take out centrifuge tube by centrifuge tube, the centrifugal 5min of 800r/min, gets supernatant and is placed in 4 DEG C of Refrigerator stores.
B) gel preparation: adopt sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) discontinuous buffer system, configuration separation gel with 10% SDS-PAGE (T=10.0%, C=2.67%, Tris 0.375mol/L, pH8.8), the concentrated glue of configuration with 4.0% SDS-PAGE (T=4.0%, C=2.67%, Tris 0.125mol/L, pH6.8), the thick 1mm of glue, two plate, sample comb is 21 holes, each sample well point sample 8-10 μ L.
C) deposition condition: electrode buffer is 25mmol/L TrisHCl, 192mmol/L glycine, 0.1%SDS, pH8.3.Two plate 30mA current stabilization electrophoresis cross liquid.
D) dyeing-decolorzing and preservation: gel 12% ethapon fixes 20min, 0.05% coomassie brilliant blue R_250 dyeed liquid, decoloured with distilled water.
E) glutenin subunit name and scoring: by above hmw glutenin subunit Electrophoretic method, obtains that the HMW-GS of new varieties consists of 1,7+9,5+10; The HMW-GS of original kind consists of 2*, 7+9,2+12; Simultaneously again grain protein content, wet gluten content and flour farinograph are carried out to new varieties, tensilometer measured and analyzed; Toasted bread, new quality reaches the strong muscle standard of GB.
2, the step of hybrid combination preparation is: plantation parent garden (if answering interval sowing when two parent's heading period differences are larger), after female parent is eared 2 days, choose maternal standard fringe (main fringe) 5 fringes, every fringe top and bottom small ear is rejected, little Hua 20 in the middle of retaining, removed by stamen in 20 little Hua, bagging is numbered; Pollinate when male parent flowering reaches 40%-50% (every day, pollinations in 8 o'clock to 12 o'clock were in the morning better, if pollen amount can adopt greatly twist with the fingers fringe method).
3, the step of cross-pollinated seed irradiation mutagenesis is: the mutagenesis of cross-pollinated seed 60Co gamma-ray irradiation.The seed moisture content of irradiation is controlled between 14%-16%; Measure irradiation chamber dose rate be 1 gray(Gy)/minute point; Irradiation seed is evenly placed in dose rate be 1 gray(Gy)/minute point on, irradiation dose is that 80 gray(Gy)s carry out irradiation mutagenesis.
4, progeny selection with the step of attribute test is: hybridize the offspring be combined with 60Co gamma-ray irradiation, according to breeding goal through too much for individual plant, single ear selection select with south numerous added-generation (if conditions permit, south numerous added-generation; If condition does not allow, also can not south numerous added-generation), to 4-5 for time hmw glutenin subunit Electrophoretic (analytical method is the same) is carried out to the seed that excellent plant or head progeny row mix receipts, by hmw glutenin subunit Electrophoretic and in conjunction with variable rate technology, to select in HMW-GS composition containing 5+10 high quality subunit and at the outstanding plant of variable rate technology or head progeny row.
5, the step that new lines is identified is: continue to add generation and selection at the outstanding plant of variable rate technology or head progeny row containing 5+10 high quality subunit by what select, until the new lines of seed selection is entered in variety test of lines after complete stability, carry out the output of 3 years, economical character, resistance, disease resistance and quality (grain protein content, wet gluten content and flour farinograph, extensograph testing, baking bread) etc. comprehensive identification, the spring wheat variety of strong muscle, high yield can be selected.
Beneficial effect of the present invention: by choosing seeds fast, cultivates the strong muscle, high yield, the precocious spring wheat variety that make new advances more easily sooner.
Embodiment recited above is only be described the preferred embodiment of the present invention, not limits the spirit and scope of the present invention.Under the prerequisite not departing from design concept of the present invention; the various modification that this area ordinary person makes technical scheme of the present invention and improvement; all should drop into protection scope of the present invention, the technology contents of request protection of the present invention, all records in detail in the claims.
Claims (6)
1. a breeding method for high-quality strong gluten spring wheat, is characterized in that: comprise hybrid strain selection, hybrid combination preparation, cross-pollinated seed irradiation mutagenesis, progeny selection and attribute test and new lines is identified.
2. the breeding method of high-quality strong gluten spring wheat according to claim 1, is characterized in that: the step that described hybrid strain is selected is:
1) rapid extraction of sample: choose 1 seed, grind and be placed in 1.5mL centrifuge tube, add 800 μ L sample buffer (62.5mmol/L Tris-HCl solution, pH value 6.8,10% glycerine, 2%SDS, 5% beta-mercaptoethanol) the rear cradle vibrate 30min of mixing immediately, puts into 90 DEG C of water-baths and extracts 5min, take out centrifuge tube by centrifuge tube, the centrifugal 5min of 800r/min, gets supernatant and is placed in 4 DEG C of Refrigerator stores.
2) gel preparation: adopt sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) discontinuous buffer system, configuration separation gel with 10% SDS-PAGE (T=10.0%, C=2.67%, Tris 0.375mol/L, pH8.8), the concentrated glue of configuration with 4.0% SDS-PAGE (T=4.0%, C=2.67%, Tris0.125mol/L, pH6.8), the thick 1mm of glue, two plate, sample comb is 21 holes, each sample well point sample 8-10 μ L.
3) deposition condition: electrode buffer is 25mmol/L TrisHCl, 192mmol/L glycine, 0.1%SDS, pH8.3.Two plate 30mA current stabilization electrophoresis cross liquid.
4) dyeing-decolorzing and preservation: gel 12% ethapon fixes 20min, 0.05% coomassie brilliant blue R_250 dyeed liquid, decoloured with distilled water.
5) glutenin subunit name and scoring: by above hmw glutenin subunit Electrophoretic method, obtains that the HMW-GS of new varieties consists of 1,7+9,5+10; The HMW-GS of original kind consists of 2*, 7+9,2+12; Simultaneously again grain protein content, wet gluten content and flour farinograph are carried out to new varieties, tensilometer measured and analyzed; Toasted bread, new quality reaches the strong muscle standard of GB.
3. the breeding method of high-quality strong gluten spring wheat according to claim 1, it is characterized in that: the step of described hybrid combination preparation is: plantation parent garden (if answering interval sowing when two parent's heading period differences are larger), after female parent is eared 2 days, choose maternal standard fringe (main fringe) 5 fringes, every fringe top and bottom small ear is rejected, little Hua 20 in the middle of retaining, removes the stamen in 20 little Hua, and bagging is numbered; Pollinate when male parent flowering reaches 40%-50% (every day, pollinations in 8 o'clock to 12 o'clock were in the morning better, if pollen amount can adopt greatly twist with the fingers fringe method).
4. the breeding method of high-quality strong gluten spring wheat according to claim 1, is characterized in that: the step of described cross-pollinated seed irradiation mutagenesis is: the mutagenesis of cross-pollinated seed 60Co gamma-ray irradiation.The seed moisture content of irradiation is controlled between 14%-16%; Measure irradiation chamber dose rate be 1 gray(Gy)/minute point; Irradiation seed is evenly placed in dose rate be 1 gray(Gy)/minute point on, irradiation dose is that 80 gray(Gy)s carry out irradiation mutagenesis.
5. the breeding method of high-quality strong gluten spring wheat according to claim 1, it is characterized in that: described progeny selection with the step of attribute test is: hybridize the offspring be combined with 60Co gamma-ray irradiation, according to breeding goal through too much for individual plant, single ear selection select with south numerous added-generation (if conditions permit, south numerous added-generation; If condition does not allow, also can not south numerous added-generation), to 4-5 for time hmw glutenin subunit Electrophoretic (analytical method is the same) is carried out to the seed that excellent plant or head progeny row mix receipts, by hmw glutenin subunit Electrophoretic and in conjunction with variable rate technology, to select in HMW-GS composition containing 5+10 high quality subunit and at the outstanding plant of variable rate technology or head progeny row.
6. the breeding method of high-quality strong gluten spring wheat according to claim 1, it is characterized in that: the step that described new lines is identified is: continue to add generation and selection at the outstanding plant of variable rate technology or head progeny row containing 5+10 high quality subunit by what select, until the new lines of seed selection is entered in variety test of lines after complete stability, carry out the output of 3 years, economical character, resistance, disease resistance and quality (grain protein content, wet gluten content and flour farinograph, extensograph testing, baking bread) etc. comprehensive identification, strong muscle can be selected, the spring wheat variety of high yield.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105949294A (en) * | 2016-07-15 | 2016-09-21 | 朱广双 | Novel method for one-time complete extraction of wheat glutenin subunits |
| CN106688877A (en) * | 2017-03-01 | 2017-05-24 | 山东省农业科学院农产品研究所 | Breeding method of red-grain early-ripe strong-gluten high-quality wheat |
| CN110214691A (en) * | 2019-07-11 | 2019-09-10 | 山东省农业科学院作物研究所 | Bad eliminate that a kind of collaboration of yield and quality of wheat improves selects excellent selection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998007747A1 (en) * | 1996-08-22 | 1998-02-26 | University Of Florida | Transformed wheat having improved breadmaking characteristics |
| CN102215668A (en) * | 2007-08-07 | 2011-10-12 | 华盛顿州立大学 | Glyphosate-tolerant wheat genotypes |
| CN102511386A (en) * | 2011-11-30 | 2012-06-27 | 张从宇 | 60Co-Gamma radiation method for breeding new wheat high-molecular-weight glutenin subunit line |
-
2015
- 2015-05-07 CN CN201510228315.6A patent/CN104839008A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998007747A1 (en) * | 1996-08-22 | 1998-02-26 | University Of Florida | Transformed wheat having improved breadmaking characteristics |
| CN102215668A (en) * | 2007-08-07 | 2011-10-12 | 华盛顿州立大学 | Glyphosate-tolerant wheat genotypes |
| CN102511386A (en) * | 2011-11-30 | 2012-06-27 | 张从宇 | 60Co-Gamma radiation method for breeding new wheat high-molecular-weight glutenin subunit line |
Non-Patent Citations (3)
| Title |
|---|
| D.-E. LAI,ET AL.: "Quality trait variations in [60Co]-irradiated wheat and high-molecular-weight glutenin subunit mutant identification", 《GENETICS AND MOLECULAR RESEARCH》 * |
| 吴振录等: "杂交与诱变相结合提高小麦育种效果的研究", 《麦类作物学报》 * |
| 张钰等: "新疆小麦高分子量麦谷蛋白亚基组成及主效亚基分析", 《核农学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105949294A (en) * | 2016-07-15 | 2016-09-21 | 朱广双 | Novel method for one-time complete extraction of wheat glutenin subunits |
| CN106688877A (en) * | 2017-03-01 | 2017-05-24 | 山东省农业科学院农产品研究所 | Breeding method of red-grain early-ripe strong-gluten high-quality wheat |
| CN110214691A (en) * | 2019-07-11 | 2019-09-10 | 山东省农业科学院作物研究所 | Bad eliminate that a kind of collaboration of yield and quality of wheat improves selects excellent selection |
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Inventor after: Fan Zheru Inventor after: Zhao Qi Inventor after: Lei Bin Inventor after: Yu Junshan Inventor after: Li Jianfeng Inventor after: Chen Jing Inventor after: Zhang Yueqiang Inventor after: Su Jingguo Inventor after: Wang Zhong Inventor after: Zhang Hua Inventor after: Zhang Hongzhi Inventor before: Li Zuxin |
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Effective date of registration: 20151229 Address after: 830000 No. 38, Nanchang Road, the Xinjiang Uygur Autonomous Region, Urumqi Applicant after: Institute of nuclear technology and Biotechnology (the Xinjiang Uygur Autonomous Region Biotechnology Research Center, Xinjiang Academy of Agricultural Sciences) Applicant after: Xinjiang gold Tianshan Mountains agricultural science and technology Co., Ltd Address before: 831100 No. 109 Beijing North Road, Changji Hui Autonomous Prefecture, the Xinjiang Uygur Autonomous Region, Changji Applicant before: Xinjiang gold Tianshan Mountains agricultural science and technology Co., Ltd |
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Application publication date: 20150819 |
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