CN104826089A - Method using fly maggot for production of immunopotentiator - Google Patents
Method using fly maggot for production of immunopotentiator Download PDFInfo
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- CN104826089A CN104826089A CN201510187164.4A CN201510187164A CN104826089A CN 104826089 A CN104826089 A CN 104826089A CN 201510187164 A CN201510187164 A CN 201510187164A CN 104826089 A CN104826089 A CN 104826089A
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- fly larvae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The invention discloses a method using fly maggot for production of an immunopotentiator, and belongs to the field of functional proteins. Fly maggot slurry as an immunopotentiator raw material is prepared by evenly mixing and crushing 90-100 parts by mass of live fly maggot, 4-6 parts by mass of wheat bran and 0.005-0.015 part by mass of vitamin C; the method using the fly maggot for production of the immunopotentiator is as follows: evenly mixing 80-100 parts by mass of the fly maggot slurry, 4-6 parts by mass of soybean lecithin, 4-6 parts by mass of radix astragali polysaccharide, 2-4 parts by mass of fly maggot puparium powder, 0.03-0.05 part by mass of vitamin C, and 0.005-0.015 part by mass of vitamin E; the fly maggot protein as a main raw material is mixed with the soybean lecithin, the vitamin C and E, the fly maggot puparium (chitosan), and the like for development of the immunopotentiator containing antibacterial substances, disease resistance of fed animals can be effectively improved, animal growth is promoted, and livestock and poultry meat quality can be significantly improved.
Description
Technical field
The present invention relates to functional protein field, be specifically related to a kind of method utilizing fly larvae to produce immunostimulant.
Background technology
Housefly belongs to Arthropoda, Insecta, Diptera, Nuscidae, has that biocycle is short, fertility is strong, to features such as adaptive capacity to environment are strong.The optimum growth temperature of housefly is generally 20-30 DEG C, and the suitableeest relative humidity is 60-80%, just can complete a generation with this understanding every 14-18d.From initial egg development to adult, generally only need 10-11d, become fly larvae by egg development, only need 4-5d, well below the biocycle of other insecticides.The fertility of housefly is very surprising, it is reported, 480 kind flies (larva weighs 0.69 gram), 16 days output larva gross weights 1086.7 grams, increase by 1575 times.Housefly is in the Conversion of energy of ecosystem, although assimilatory efficiency is only about mammiferous half, its production efficiency be but mammiferous 15-40 doubly, this be up to now other animals incomparable.Musca omnivorous insect, therefore the agricultural byproducts leftover bits and pieces such as Testa oryzae, Testa Tritici, bean dregs, distiller grains and industrial residue and fowl and animal excrement etc. all can be used for the raising of fly larvae.Fly larvae is the larva of housefly, nutritious, and its gross protein value is up to 56%-63% (average out to 59.5%), higher than bean cake, soybean cake, Pupa bombycis and domestic fish meal, almost can compare favourably with imported fish meal.And the nutritional labeling of fly larvae is also comparatively comprehensive, containing kind of the essential amino acids of 7 required for animal as lysine and methionine etc., its TEAA is 1.03 times of fish flour, methionine content is 2.20 times of fish flour, lysine content is 1.10 times of fish flour, can be used as the high-quality feed supplement of a lot of economic animal.
Housefly has unique immunologic function, has abundant antibacterial peptide, have extremely strong sterilization and anti-virus ability in its body.Survival and reproduction in the environment that the existence of antibacterial peptide enables zootic fly larvae exist in a large number at pathogenic microorganism, and to human non-toxic's side effect, life-time service can not develop immunity to drugs.Extract exploitation fly maggot protein antibacterial peptide and decrease antibiotic use in livestock breeding industry, be one of effective way solving the problems such as drug resistance, improve livestock-raising benefit, for green organic agricultural creates conditions.Fly maggot protein, as the high-quality feed supplement of many economic animals, can improve the speed of growth of animal, also can effectively strengthen its resistance against diseases.
Summary of the invention
The object of the present invention is to provide a kind of fly larvae serosity as immunostimulant raw material, and the immunostimulant prepared based on it; The present invention is also to provide a kind of method utilizing fly larvae to produce immunostimulant.
Object of the present invention is achieved through the following technical solutions:
As a fly larvae serosity for immunostimulant raw material, comprise following in the component of mass parts: the fly larvae 90-100 part lived, wheat bran 4-6 part, vitamin C 0.005-0.015 part.
The fly larvae of described work is by with milk powder: brown sugar: the diet housefly of water 1: 1: 5 composition in mass ratio obtains.
The preparation method of described fly larvae serosity, comprises the steps:, by being added in milling apparatus after the mixing of the fly larvae of the work of described mass parts, wheat bran and vitamin C, to grind to form fly larvae serosity.
A kind of immunostimulant, comprises above-mentioned fly larvae serosity.
Preferably, described immunostimulant comprises following in the component of mass parts: above-mentioned fly larvae serosity 80-100 part, soybean lecithin 4-6 part, Huang Shi polysaccharide 4-6 part, maggot shell powder 2-4 part, vitamin C 0.03-0.05 part, vitamin E 0.005-0.015 part.
Utilize fly larvae to produce a method for above-mentioned immunostimulant, comprise the steps: the fly larvae serosity of described mass parts, soybean lecithin, Huang Shi polysaccharide, fly larvae puparium powder, vitamin C and vitamin E mix homogeneously to obtain.
The present invention is that primary raw material is aided with soybean lecithin, vitamin C and E, fly larvae puparium (chitin) etc. with fly maggot protein, develop a kind of immunostimulant containing antibiotic substance, effectively can improve by the premunition of letting animals feed, promote growth of animal, significantly improve the meat of poultry.Wherein comprise chitin in fly larvae puparium, chitin is for containing ammonia polysaccharide natural bioactive thing, show by detecting analysis, with immunostimulant domesticated animal of the present invention not only containing the material that any antibiotic, the parahormone of synthesis and heavy metal etc. may be harmful to human body, and its protein content is significantly higher than conventional animal.
Detailed description of the invention
Following examples are used for further illustrating the present invention, but should not be construed as limitation of the present invention.If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art; The number of each raw material is mass fraction.
The preparation of embodiment 1 immunostimulant
(1) preparation of fly larvae serosity
In the sarong of 50cm × 50cm × 50cm, raise housefly, housefly feed formula is milk powder: brown sugar: water=1: 1: 5 (mass ratio), gained fly larvae is for the preparation of fly larvae serosity.
Be added to after the fly larvae of 95 parts of work, 5 parts of wheat brans and 0.01 part of vitamin C (Henan Rong Teng food food additive company limited) mixing in milling apparatus (common refiner), under room temperature, grind to form fly larvae serosity.
After testing, in fly larvae serosity, (dry weight) gross protein value up to 56%-63% (average out to 59.5%), crude fat 2.6%-5.0%, ash 3%, calcium phosphorus 0.5%-2.0%, total amino acid content 55%-62%.
(2) preparation of immunostimulant
90 parts of fly larvae serosity obtained above, 5 parts of soybean lecithins (Henan Rong Teng food food additive company limited), 5 parts of Huang Shi polysaccharide (Hui Cheng bio tech ltd, Shaanxi), 3 parts of the fly larvae puparium pulverized, 0.04 part of vitamin C (Henan Rong Teng food food additive company limited) and 0.01 part of vitamin E (the moral commerce and trade company limited of Henan Sheng) mix homogeneously are obtained immunostimulant.
The effect of Anguillar japonica raised by embodiment 2 immunostimulant
Different according to the feedstuff of raising Anguillar japonica, be divided into test group, matched group 1, matched group 2.Wherein, the immunostimulant prepared by imported fish meal and embodiment 1 of test group feedstuff in mass ratio 8:2 mixes and forms, matched group 1 feedstuff for use imported fish meal completely, matched group 2 feedstuff be imported fish meal with common Flyblow albumen powder in mass ratio 8:2 mix and form.Imported fish meal in the present embodiment and following embodiment is Dongguan City Lin Shi feed advance company limited product, and common Flyblow albumen powder is insecticide bio tech ltd, Yunnan product.
Will ensure quality of the fodder when throwing something and feeding, soft or hard appropriateness wanted by the feedstuff modulated, and amount of water is 1.2 times of feeding quality, and throwing the amount of raising is 2% of Anguillar japonica body weight.When every day the morning 8, afternoon 4 time about respectively throw something and feed 1 time, throw something and feed the morning and account for 40%, throw something and feed afternoon and account for 60%.
The present embodiment in 10 days to 2014 August in 2014 on JIUYUE within 10, carry out in Wuhan Ke Yang biological engineering company limited, the results are shown in Table 1.
Table 1
Through a test in month, test group is compared with matched group 2 with matched group 1, the Anguillar japonica growth rate of immunostimulant of throwing something and feeding is fast, balanced growth speed improves 4.93% and 1.9% respectively, simultaneously, the price of deed improves, and feedstuff-meat ratio reduces by 0.06 and 0.07 respectively, and in muscle, protein content improves 4.86% and 3.17% respectively.
The effect of laying hen raised by embodiment 3 immunostimulant
Different according to the feedstuff of raising laying hen, be divided into test group, matched group 1, matched group 2.Wherein, the immunostimulant prepared by imported fish meal and embodiment 1 of test group feedstuff in mass ratio 8:2 mixes and forms, matched group 1 feedstuff for use imported fish meal completely, matched group 2 feedstuff be imported fish meal with common Flyblow albumen powder in mass ratio 8:2 mix and form.Imported fish meal in the present embodiment is Dongguan City Lin Shi feed advance company limited product, and common Flyblow albumen powder is insecticide bio tech ltd, Yunnan product.
The present embodiment carries out on August 10,10 days to 2014 May in 2013 in Wuhan Ke Yang biological engineering company limited, the results are shown in Table 2.
Table 2
By above-mentioned test, test group is compared with matched group 2 with matched group 1, and the laying rate of laying hen of immunostimulant of throwing something and feeding increases by 18.9% and 7.7% respectively, simultaneously, the price of deed improves, and feedstuff-meat ratio reduces by 0.51 and 0.19 respectively, and the protein content in muscle improves 3.1% and 1.3% respectively.
Embodiment 4 immunostimulant is to prawn growth promoter and suppress viral pathogenetic effect
Different according to the feedstuff of raising prawn, be divided into test group, matched group 1, matched group 2.Wherein, the immunostimulant prepared by prawn feed and embodiment 1 of test group feedstuff in mass ratio 8:2 mixes and forms, matched group 1 feedstuff for use prawn feed completely, matched group 2 feedstuff be prawn feed with common Flyblow albumen powder in mass ratio 8:2 mix and form.Prawn feed in the present embodiment is Kangda Forage Co., Ltd., Huaian's product, and common Flyblow albumen powder is insecticide bio tech ltd, Yunnan product.
Every daily ration of feeding is thrown according to body weight difference, and feeding quality of throwing something and feeding is 5% of body weight.Bait throwing in every day 2 times, throws something and feeds when the morning 7, accounts for 30% of daily ration, feeding quantity, and throw something and feed when afternoon 6, daily ration, feeding quantity accounts for 70%.
The present embodiment carries out in Xing Wuyuan bio tech ltd, Xianning on January 22nd, 23 days 1 in JIUYUE in 2013, raises prawn respectively in Tu Tang and feed coefficient, the results are shown in Table 3 (Tu Tang) and table 4 (feed coefficient).
Table 3
Table 4
Shown by test, immunostimulant effectively can suppress the generation of prawn virus disease, significantly promotes the growth of prawn simultaneously, improves output.In the native pool, test group is compared to matched group 1 and matched group 2, and mortality rate declines 42% and 8.3% respectively, and prawn rate of rise improves 104.9% and 20.7% respectively.In feed coefficient, test group is compared to matched group 1 and matched group 2, and prawn crop improves 30.5% and 10.1% respectively, and the price of deed improves, and feedstuff-meat ratio reduces by 0.06 and 0.03 respectively, and in muscle, protein content improves 3.45% and 2.35% respectively.
Claims (6)
1., as a fly larvae serosity for immunostimulant raw material, it is characterized in that comprising as follows in the component of mass parts: the fly larvae 90-100 part lived, wheat bran 4-6 part, vitamin C 0.005-0.015 part.
2. fly larvae serosity according to claim 1, is characterized in that: the fly larvae of described work is by with milk powder: brown sugar: the diet housefly of water 1: 1: 5 composition in mass ratio obtains.
3. the preparation method of the fly larvae serosity described in claim 1 or 2, is characterized in that comprising the steps:, by being added in milling apparatus after the mixing of the fly larvae of the work of described mass parts, wheat bran and vitamin C, to grind to form fly larvae serosity.
4. an immunostimulant, is characterized in that: comprise the fly larvae serosity described in claim 1 or 2.
5. immunostimulant according to claim 4, it is characterized in that comprising as follows in the component of mass parts: the fly larvae serosity 80-100 part described in claim 1 or 2, soybean lecithin 4-6 part, Huang Shi polysaccharide 4-6 part, maggot shell powder 2-4 part, vitamin C 0.03-0.05 part, vitamin E 0.005-0.015 part.
6. the preparation method of immunostimulant according to claim 5, is characterized in that comprising the steps: the fly larvae serosity of described mass parts, soybean lecithin, Huang Shi polysaccharide, fly larvae puparium powder, vitamin C and vitamin E mix homogeneously to obtain.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041345A (en) * | 2017-12-11 | 2018-05-18 | 中山大学 | A kind of America of disease-resistant growth-promoting is into eel fly-maggot protein fodder additive and its preparation method and application |
| CN108041346A (en) * | 2017-12-11 | 2018-05-18 | 中山大学 | A kind of America elver fly-maggot protein fodder additive and its preparation method and application of disease-resistant growth-promoting |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1269153A (en) * | 1999-04-05 | 2000-10-11 | 唐耿吉 | Production process of maggot protein additivie for fish feed |
| CN104206360A (en) * | 2014-09-04 | 2014-12-17 | 江门鑫肽生物蛋白有限公司 | Quick flyblow hatching method |
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2015
- 2015-04-20 CN CN201510187164.4A patent/CN104826089B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1269153A (en) * | 1999-04-05 | 2000-10-11 | 唐耿吉 | Production process of maggot protein additivie for fish feed |
| CN104206360A (en) * | 2014-09-04 | 2014-12-17 | 江门鑫肽生物蛋白有限公司 | Quick flyblow hatching method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041345A (en) * | 2017-12-11 | 2018-05-18 | 中山大学 | A kind of America of disease-resistant growth-promoting is into eel fly-maggot protein fodder additive and its preparation method and application |
| CN108041346A (en) * | 2017-12-11 | 2018-05-18 | 中山大学 | A kind of America elver fly-maggot protein fodder additive and its preparation method and application of disease-resistant growth-promoting |
| CN108041345B (en) * | 2017-12-11 | 2021-04-09 | 中山大学 | Disease-resistant growth-promoting maggot protein feed additive for adult American eels and preparation method and application thereof |
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