CN104823858A - Disposable air-lifting type plant seed tissue culture reactor - Google Patents
Disposable air-lifting type plant seed tissue culture reactor Download PDFInfo
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- CN104823858A CN104823858A CN201510268034.3A CN201510268034A CN104823858A CN 104823858 A CN104823858 A CN 104823858A CN 201510268034 A CN201510268034 A CN 201510268034A CN 104823858 A CN104823858 A CN 104823858A
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Abstract
The invention provides a disposable air-lifting type plant seed tissue culture reactor and belongs to the technical field of plant tissue culture. The disposable air-lifting type plant seed tissue culture reactor has a simple structure, is easy to mount and is easy to sterilize, and culture products are easy to harvest. A reactor main body is a sealed cavity made of a medical plastic thin film; the reactor main body is arranged on a bracket; an aeration sheet is fixed on an aeration nozzle; the aeration nozzle is in hot melting connection with the bottom of the reactor main body; an air outlet end of a compressed air inlet pipeline is fixedly mounted on the aeration nozzle; the upper end of the outer surface of the side wall of the reactor main body is fixedly connected with one end of a pH probe pipeline, one end of an oxygen dissolving probe pipeline and a liquid outlet end of a liquid inlet pipeline; the pH probe pipeline, the oxygen dissolving probe pipeline and the liquid feeding pipeline are communicated with an inner cavity of the reactor main body respectively; the outer surface of the top of the reactor main body is fixedly connected with an air inlet end of an air outlet pipeline; a blind plate is mounted on the liquid inlet pipeline; and the air outlet end of the air outlet pipeline is connected with a filter. The disposable air-lifting type plant seed tissue culture reactor is used for plant tissue culture.
Description
Technical field
The present invention relates to a kind of Plant Tissue Breeding reactor, belong to field of plant tissue culture technique.
Background technology
After 20 century 70s, the more employing airlift bioreactor of cultivation of plant cell/tissue and animal organ.At present, and the agent structure of existing airlift bioreactor is many is made up of stainless steel or glass material.Stainless steel bioreactor culture is effective, but disposablely to have high input, reactor vapor sterilization process length consuming time, power consumption greatly, not easy to clean, not easily gather in the crops cultured products; Although glass material reactor is convenient to observe, frangible, dangerous, withstand voltage weak effect, bioreactor culture small scale, be especially not easy to grope reactor design technology parameter in reactor design initial stage.In addition existing airlift bioreactor inside is provided with the parts such as multilayer guide ring, and complex structure, easily installs, maintenance, difficult in maintenance.
Summary of the invention
The object of the invention is to the deficiency overcoming existing Plant Tissue Breeding reactor, provide a kind of structure simply, easily to install, be easy to sterilizing, easily the disposable pneumo lift-type plant seed tissue of results cultured products cultivate reactor.
The present invention solves the problem the technical scheme taked respectively:
Reactor is cultivated by disposable pneumo lift-type plant seed tissue, comprise reactor body, multiple compressed air inlet pipe road, multiple aeration nozzle, multiple aerator disk, PH pops one's head in pipeline, dissolved oxygen probe pipeline, water influent pipeline, blind plate, outlet pipe, support 1 and filter, described reactor body is the closed cavity be made up of Medical plastic film, reactor body is arranged on support, described aerator disk is fixed in aeration nozzle, hot-melt adhesive paste bottom aeration nozzle and reactor body, the outlet side on described compressed air inlet pipe road is fixedly mounted in aeration nozzle, compressed air inlet pipe road and aeration nozzle one_to_one corresponding are arranged, compressed air inlet pipe road is communicated with the inner chamber of reactor body by aeration nozzle and aerator disk, pop one's head in PH respectively pipeline, one end of dissolved oxygen probe pipeline and the outlet end of water influent pipeline of the upper end of the wall outer surface of reactor body is fixedly connected with, PH probe pipeline, dissolved oxygen probe pipeline and water influent pipeline all communicate with the inner chamber of reactor body, reactor body outer surface of cupular part is fixedly connected with the inlet end of outlet pipe, described water influent pipeline is provided with blind plate, the outlet side of outlet pipe 9 is connected with filter.
The present invention relative to the beneficial effect of prior art is:
1. easily sterilizing; Reactor is cultivated by disposable pneumo lift-type plant seed tissue of the present invention can realize sterilizing after Co 60 irradiates, and can come into operation fast.
2. easily gather in the crops cultured products; Cultivation completes wild Oryza species and is first discharged by compressed air inlet pipe road, bottom, disposable pneumo lift-type plant seed tissue is cultivated after reactor top cuts open and cultured products can be taken out.
3. save a large amount of reactor cleaning cleaning times; Compared with traditional stainless steel, glass biological reaction device, the cleaning before using without the need to reactor and after results is cleaned, and has saved the plenty of time.
4. disposable pneumo lift-type plant seed tissue of the present invention cultivation reactor has structure simply, easily installs, non-maintaining feature.
Accompanying drawing explanation
Fig. 1 is the structural representation that reactor is cultivated by disposable pneumo lift-type plant seed tissue of the present invention;
Fig. 2 is the A portion enlarged drawing of Fig. 1;
Fig. 3 is the enlarged drawing of the dissolved oxygen probe pipeline in Fig. 1;
Fig. 4 is the enlarged drawing of the PH probe pipeline in Fig. 1.
The component names related in above-mentioned figure and label are respectively:
Reactor body 1, compressed air inlet pipe road 2, aeration nozzle 3, aerator disk 4, PH probe pipeline 5, PH probe 5-1, connecting line one 5-2, dissolved oxygen probe pipeline 6, dissolved oxygen probe 6-1, connecting line two 6-2, water influent pipeline 7, blind plate 8, outlet pipe 9, dull and stereotyped folder 10, support 11, filter 12.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail: the present embodiment is implemented under premised on technical solution of the present invention, gives detailed embodiment, but protection scope of the present invention is not limited to following real legend.
Embodiment one: as shown in Fig. 1 ~ Fig. 4, reactor is cultivated by disposable pneumo lift-type plant seed tissue, comprise reactor body 1, multiple compressed air inlet pipe road 2, multiple aeration nozzle 3, multiple aerator disk 4, PH pops one's head in pipeline 5, dissolved oxygen probe pipeline 6, water influent pipeline 7, blind plate 8, outlet pipe 9, support 11 and filter 12, described reactor body 1 is the closed cavity be made up of Medical plastic film, reactor body 1 is arranged on support 11, described aerator disk 4 is fixed in aeration nozzle 3, hot-melt adhesive paste bottom aeration nozzle 3 and reactor body 1, the outlet side on described compressed air inlet pipe road 2 is fixedly mounted in aeration nozzle 3, compressed air inlet pipe road 2 and aeration nozzle 3 one_to_one corresponding are arranged, compressed air inlet pipe road 2 is communicated with the inner chamber of reactor body 1 by aeration nozzle 3 and aerator disk 4, pop one's head in PH respectively pipeline 5, one end of dissolved oxygen probe pipeline 6 and the outlet end of water influent pipeline 7 of the upper end of the wall outer surface of reactor body 1 is fixedly connected with, PH probe pipeline 5, dissolved oxygen probe pipeline 6 and water influent pipeline 7 all communicate with the inner chamber of reactor body 1, reactor body 1 outer surface of cupular part is fixedly connected with the inlet end of outlet pipe 9, described water influent pipeline 7 is provided with blind plate 8, the outlet side of outlet pipe 9 is connected with filter 12.Its objective is that preventing extraneous air from entering reactor pollutes.
Described PH probe pipeline 5 comprises PH probe 5-1, connecting line one 5-2 and nylon cable tie one, described connecting line one 5-2 one end and the PH 5-1 that pops one's head in is fixedly connected with by nylon cable tie one, and the described PH 5-1 that pops one's head in is fixedly connected with the upper end of the wall outer surface of reactor body 1.The effect of PH probe 5-1 is the PH numerical value for monitoring in incubation.
Described dissolved oxygen probe pipeline 6 comprises dissolved oxygen probe 6-1, connecting line two 6-2 and nylon cable tie two, described connecting line two 6-2 one end is fixedly connected with by nylon cable tie two with dissolved oxygen probe 6-1, and described dissolved oxygen probe 6-1 is fixedly connected with the upper end of the wall outer surface of reactor body 1.The effect of dissolved oxygen probe 6-1 is the dissolved oxygen numerical value for monitoring in incubation.
Each compressed air inlet pipe road 2 is all provided with dull and stereotyped folder 10.For controlling the air inflow entering reactor body 1 compressed air.
The quantity on described compressed air inlet pipe road 2 is seven.The needs of Plant Tissue Breeding can be met.
The course of work: as shown in Fig. 1 ~ Fig. 4, disposable pneumo lift-type plant seed tissue of the present invention cultivates reactor and can directly use after Co 60 radiation sterilization, during inoculation, seed and medium enter in reactor body 1 by water influent pipeline 7, and utilize the PH numerical value that PH pops one's head in 5-1 monitoring incubation, utilize the dissolved oxygen numerical value in dissolved oxygen probe 6-1 monitoring incubation, dull and stereotyped folder 10 is opened after having inoculated, compressed air enters in reactor body 1 by compressed air inlet pipe road 2, aeration nozzle 3, through outlet pipe 9, discharged by filter 12.After having inoculated, the blind plate 8 be arranged in water influent pipeline 7 is utilized to be shut by liquid outlet.
Reactor body 1 volume of the present invention is 1000L.
Claims (4)
1. reactor is cultivated by a disposable pneumo lift-type plant seed tissue, comprise reactor body (1), multiple compressed air inlet pipe road (2), multiple aeration nozzle (3), multiple aerator disk (4), PH probe pipeline (5), dissolved oxygen probe pipeline (6), water influent pipeline (7), blind plate (8), outlet pipe (9), support (11) and filter (12), it is characterized in that: described reactor body (1) is the closed cavity be made up of Medical plastic film, reactor body (1) is arranged on support (11), described aerator disk (4) is fixed in aeration nozzle (3), aeration nozzle (3) and reactor body (1) bottom hot-melt adhesive paste, the outlet side on described compressed air inlet pipe road (2) is fixedly mounted in aeration nozzle (3), compressed air inlet pipe road (2) and aeration nozzle (3) one_to_one corresponding are arranged, compressed air inlet pipe road (2) is communicated with the inner chamber of reactor body (1) by aeration nozzle (3) and aerator disk (4), pop one's head in PH respectively pipeline (5), one end of dissolved oxygen probe pipeline (6) and the outlet end of water influent pipeline (7) of the upper end of the wall outer surface of reactor body (1) is fixedly connected with, PH probe pipeline (5), dissolved oxygen probe pipeline (6) and water influent pipeline (7) all communicate with the inner chamber of reactor body (1), reactor body (1) outer surface of cupular part is fixedly connected with the inlet end of outlet pipe (9), described water influent pipeline (7) is provided with blind plate (8), the outlet side of outlet pipe (9) is connected with filter (12).
2. reactor is cultivated by disposable pneumo lift-type plant seed tissue according to claim 1, it is characterized in that: described PH probe pipeline (5) comprises PH probe (5-1), connecting line one (5-2) and nylon cable tie one, described connecting line one (5-2) one end pop one's head in PH (5-1) be fixedly connected with by nylon cable tie one, described PH probe (5-1) is fixedly connected with the upper end of the wall outer surface of reactor body (1).
3. reactor is cultivated by disposable pneumo lift-type plant seed tissue according to claim 2, it is characterized in that: described dissolved oxygen probe pipeline (6) comprises dissolved oxygen probe (6-1), connecting line two (6-2) and nylon cable tie two, described connecting line two (6-2) one end is fixedly connected with by nylon cable tie two with dissolved oxygen probe (6-1), and described dissolved oxygen probe (6-1) is fixedly connected with the upper end of the wall outer surface of reactor body (1).
4. reactor is cultivated by the disposable pneumo lift-type plant seed tissue according to claim 1,2 or 3, it is characterized in that: each compressed air inlet pipe road (2) is all provided with dull and stereotyped folder (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510268034.3A CN104823858A (en) | 2015-05-25 | 2015-05-25 | Disposable air-lifting type plant seed tissue culture reactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510268034.3A CN104823858A (en) | 2015-05-25 | 2015-05-25 | Disposable air-lifting type plant seed tissue culture reactor |
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| CN104823858A true CN104823858A (en) | 2015-08-12 |
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| CN201510268034.3A Pending CN104823858A (en) | 2015-05-25 | 2015-05-25 | Disposable air-lifting type plant seed tissue culture reactor |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6420173B1 (en) * | 2000-05-24 | 2002-07-16 | Microplants Co., Ltd. | Method for mass production and storage of seedlings of Eleutherococcus senticosus (Siberian ginseng) through cell culturing |
| CN203065461U (en) * | 2012-10-22 | 2013-07-17 | 中国石油化工股份有限公司 | Internal loop airlift microbial culture reactor |
| CN103224882A (en) * | 2012-01-31 | 2013-07-31 | 天津艾赛博生物技术有限公司 | Disposable wave type bioreactor with aerating apparatus |
| CN204690015U (en) * | 2015-05-25 | 2015-10-07 | 固元本草汉方生物科技股份有限公司 | Reactor is cultivated by disposable pneumo lift-type plant seed tissue |
-
2015
- 2015-05-25 CN CN201510268034.3A patent/CN104823858A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6420173B1 (en) * | 2000-05-24 | 2002-07-16 | Microplants Co., Ltd. | Method for mass production and storage of seedlings of Eleutherococcus senticosus (Siberian ginseng) through cell culturing |
| CN103224882A (en) * | 2012-01-31 | 2013-07-31 | 天津艾赛博生物技术有限公司 | Disposable wave type bioreactor with aerating apparatus |
| CN203065461U (en) * | 2012-10-22 | 2013-07-17 | 中国石油化工股份有限公司 | Internal loop airlift microbial culture reactor |
| CN204690015U (en) * | 2015-05-25 | 2015-10-07 | 固元本草汉方生物科技股份有限公司 | Reactor is cultivated by disposable pneumo lift-type plant seed tissue |
Non-Patent Citations (2)
| Title |
|---|
| WILLIAM B. ZIMMERMAN等: "On the design and simulation of an airlift loop bioreactor with microbubble generation by fluidic oscillation", 《FOOD AND BIOPRODUCTS PROCESSING》 * |
| 成喜雨等: "植物组织培养生物反应器技术研究进展", 《生物加工过程》 * |
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Application publication date: 20150812 |
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