CN104817706A - Preparation method for Setipinna tenuifilis protein nano-silver antibacterial film - Google Patents
Preparation method for Setipinna tenuifilis protein nano-silver antibacterial film Download PDFInfo
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- CN104817706A CN104817706A CN201510107297.6A CN201510107297A CN104817706A CN 104817706 A CN104817706 A CN 104817706A CN 201510107297 A CN201510107297 A CN 201510107297A CN 104817706 A CN104817706 A CN 104817706A
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Abstract
The invention relates to a preparation method for a Setipinna tenuifilis protein nano-silver antibacterial film. The preparation method comprises the steps of: preparation of a solution A: adding gelatin into a Setipinna tenuifilis protein antibacterial peptide solution to prepare a gelatin solution, adding glycerin into the gelatin solution, after complete dissolution, mixing the mixture evenly to obtain the solution A; preparation of a solution B: preparing a silver nitrate solution; film making: taking the solution A, adding the solution B, and conducting film coating by a coating technique, thus obtaining the Setipinna tenuifilis protein nano-silver antibacterial film. Silver nitrate is reduced into silver ions by means of the reducing effect of the Setipinna tenuifilis protein antibacterial peptide solution (mild reaction conditions), then the silver ions are dispersed into the gelatin (with rich sources and high safety) solution, and the interaction of the Setipinna tenuifilis protein antibacterial peptide solution and gelatin improves the stretchability and toughness of the final film.
Description
Technical field
The invention belongs to anti-biotic material technical field, particularly relate to a kind of antibacterial efficient, pollute few, reaction conditions is gentle, the preparation method of the yellow crucian carp protein nano silver antibacterial film of good energy-conserving effect.
Background technology
Pathogenic microorganism is ubiquitous, all-pervasive, their propagation and spread the health that the mankind in serious threat.Along with a large amount of uses of the chemicalses such as microbiotic, sterilizing agent and sterilant, the bacterial dissociation population caused by resistance gets more and more.
Anti-biotic material refers to the class functional materials can killing or hinder microbial growth and activity thereof.Along with development in science and technology and the requirement of people to healthy living more and more higher, antimicrobial technology and anti-biotic material receive to be paid close attention to more and more widely.Anti-biotic material relatively more conventional at present comprises inorganic system and the large class of organic system two.Inorganic owner will refer to all kinds of metallics and its esters, mainly comprises metal_based material, natural mineral, carbon fiber etc.; The principal item of organic system has quaternary ammonium salt, biguanides, alcohols, phenols, pyridines, imidazoles, halogen amine etc.Inorganic antibacterial material, compared with organic anti-bacterial material, has wide spectrum, durable, and the feature of safety, wherein silver is that anti-biotic material has become the inorganic antibacterial material be most widely used at present.
Nanometer silver is the novel product developed based on nanotechnology, in electricity, optics and catalysis etc., have very excellent characteristic.Simultaneously due to surface effects, volume effect, quantum size effect and macro quanta tunnel effect, nanometer silver has the incomparable antibacterial effect of conventional silver system anti-biotic material, the nanometer silver of minute quantity can make microorganism comprise the lower level of the Growth and reproduction maintenances such as bacterium, fungi, yeast, and effect is lasting.Based on this, nano silver antibacterial material has application prospect widely, comprises various antibacterial dress ornament, antibacterials, toy for children, personal-care supplies etc.
China Patent Publication No. CN1742582A, publication date on March 8th, 2006, name is called a kind of photocatalyst-type anti-biotic material preparation method, and this application case discloses a kind of photocatalyst-type anti-biotic material preparation method, comprise A, preparation step, be prepared antiseptic solution and material surface pre-treatment respectively; B, material surface coating antiseptic solution step; C, drying step, make to be coated on material surface attachment and form antibacterial film; D, solidification treatment step, promote the combination on antibiotic layer and collective surface by thermal treatment, photocatalysis antibacterial once realized anatase crystal transformation simultaneously.Its weak point is, the method step much complex, and thermal treatment power consumption is more.
Summary of the invention
The object of the present invention is to provide that a kind of preparation process is simple, energy-conserving and environment-protective, there is the preparation method of the yellow crucian carp protein nano silver antibacterial film of good anti-microbial property.
To achieve these goals, the present invention is by the following technical solutions:
A preparation method for yellow crucian carp protein nano silver antibacterial film, described preparation method comprises the steps:
A) A liquid is prepared: in yellow crucian carp albumen antibacterial peptide liquid, add gelatin, be mixed with gelatin solution, in gelatin solution, add glycerine, abundant dissolving and mixing, obtain A liquid;
B) B liquid is prepared: configuration concentration is the silver nitrate solution of 500 μ g/mL;
C) masking: get the A liquid that step a) obtains, stir at 35-45 DEG C of lower magnetic force and be incubated 10-20min, then the B liquid that step b) obtains is added, mixed solution is temperature constant magnetic stirring 2.5-3.2h at 35-45 DEG C, mixed solution is adopted coating method film, dry 24-26h, obtains yellow crucian carp protein nano silver antibacterial film.In the technical program, in step c) during mixed solution temperature constant magnetic stirring, if temperature is lower than 30 DEG C, gelatin can solidify, and the too high yellow crucian carp albumen antibacterial peptide liquid of temperature can deepen through long-time heating color, it is then because fully can form nanometer silver within this time period that churning time is decided to be 2.5-3.2h.
As preferably, in step a), the concentration of gelatin solution is 20-25%.
As preferably, the add-on of glycerine is the 25%-27% of gelatin weight.In the technical program, glycerine add the stretchiness that effectively can improve film.
As preferably, in step c), the add-on of B liquid is identical with the volume of A liquid.
As preferably, the feed postition of B liquid is for dropwise to add.
As preferably, the thickness of film is 1-2mm.
The invention has the beneficial effects as follows:
1) reaction conditions of the present invention is gentle, without the need to severe condition such as High Temperature High Pressure, and good energy-conserving effect;
2) yellow crucian carp protein nano silver antibacterial film good stability prepared by the present invention, is widely used, efficiently antibacterial, antibacterial wide spectrum;
3) abundant raw material source of the present invention, security is high, pollutes few, environmental protection;
4) reductive action of yellow crucian carp albumen antibacterial peptide liquid is utilized to be silver atoms (reaction conditions is gentle) by silver nitrate reduction, then be distributed in gelatin (abundance, and security is high) in liquid, the stretchiness and toughness that improve most telolemma and yellow crucian carp albumen antibacterial peptide liquid and gelatin interact.
Accompanying drawing explanation
Fig. 1 is embodiment 1 uv-vis spectra scintigram.
Fig. 2 is the antibacterial inhibition figure of embodiment 1.
Fig. 3 is the antibacterial inhibition figure of embodiment 2.
Embodiment
Below in conjunction with specific embodiment, the present invention is further explained:
The preparation of yellow crucian carp albumen antibacterial peptide liquid, yellow crucian carp removes internal organ afterwash, and tissue mashing machine smashs to pieces, deionized water mixing is added according to the ratio of 1:4, mixed serum acid for adjusting pH value is to 2.0, and 37 DEG C of insulation 10min, add the stomach en-of flesh of fish quality 0.5% and stir.Front 30min in enzymolysis process adds hydrochloric acid or alkali every 15min and keeps pH value 2.0.After reaction terminates, 95-100 DEG C of heating 10min passivation is gone out enzyme, and mixed solution is through the centrifugal 10min of low temperature (4 DEG C) 7000r/min, discard upper strata oil film and bottom residues, stay middle enzymolysis solution alkali adjust ph to 5.0, then can obtain yellow crucian carp albumen antibacterial peptide liquid after filtration ,-20 DEG C of preservations are for subsequent use.
Embodiment 1
A preparation method for yellow crucian carp protein nano silver antibacterial film, described preparation method comprises the steps:
A) A liquid is prepared: in yellow crucian carp albumen antibacterial peptide liquid, add gelatin, be mixed with the gelatin solution that concentration is 20%, in gelatin solution, add glycerine, abundant dissolving and mixing, obtain A liquid; The add-on of glycerine is 25% of gelatin weight;
B) B liquid is prepared: the silver nitrate solution with deionized water configuration concentration being 500 μ g/mL;
C) masking: get the A liquid that 10mL step a) obtains, stir at 40 DEG C of lower magnetic forces and be incubated 10min, then the B liquid that step b) obtains dropwise is added, the add-on of B liquid is identical with the volume of A liquid, and mixed solution is temperature constant magnetic stirring 3h at 40 DEG C, gets one piece, clean glass, adopt coating method film, the thickness of film is 1mm, dry 24h, obtains yellow crucian carp protein nano silver antibacterial film.
Embodiment 2
A preparation method for yellow crucian carp protein nano silver antibacterial film, described preparation method comprises the steps:
A) A liquid is prepared: in yellow crucian carp albumen antibacterial peptide liquid, add gelatin, be mixed with the gelatin solution that concentration is 22%, in gelatin solution, add glycerine, abundant dissolving and mixing, obtain A liquid; The add-on of glycerine is 26% of gelatin weight;
B) B liquid is prepared: the silver nitrate solution with deionized water configuration concentration being 500 μ g/mL;
C) masking: get the A liquid that 10mL step a) obtains, stir at 35 DEG C of lower magnetic forces and be incubated 15min, then the B liquid that step b) obtains dropwise is added, the add-on of B liquid is identical with the volume of A liquid, and mixed solution is temperature constant magnetic stirring 2.5h at 35 DEG C, gets one piece, clean glass, adopt coating method film, the thickness of film is 2mm, dry 25h, obtains yellow crucian carp protein nano silver antibacterial film.
Embodiment 3
A preparation method for yellow crucian carp protein nano silver antibacterial film, described preparation method comprises the steps:
A) A liquid is prepared: in yellow crucian carp albumen antibacterial peptide liquid, add gelatin, be mixed with the gelatin solution that concentration is 25%, in gelatin solution, add glycerine, abundant dissolving and mixing, obtain A liquid; The add-on of glycerine is 27% of gelatin weight;
B) B liquid is prepared: the silver nitrate solution with deionized water configuration concentration being 500 μ g/mL;
C) masking: get the A liquid that 10mL step a) obtains, stir at 45 DEG C of lower magnetic forces and be incubated 20min, then the B liquid that step b) obtains dropwise is added, the add-on of B liquid is identical with the volume of A liquid, and mixed solution is temperature constant magnetic stirring 3.2h at 45 DEG C, gets one piece, clean glass, adopt coating method film, the thickness of film is 1.5mm, dry 26h, obtains yellow crucian carp protein nano silver antibacterial film.
Fig. 1 be nano silver colloidal sol generally uv-vis spectra scanning in 400 ~ 450nm scope have charateristic avsorption band.From Fig. 1 result: yellow crucian carp albumen antibacterial peptide/gelatin/AgNPs occurs absorption peak near 450nm, shows that this system contains nanometer silver, that is: the load nano silver film that yellow crucian carp albumen antibacterial peptide/gelatin/AgNPs is formed.
In Fig. 2 and Fig. 3, antibacterial result shows the nanometer film that yellow crucian carp albumen antibacterial peptide liquid/gelatin/AgNPs formed and has very strong fungistatic effect to food pathogenic Enterohemorrhagic E.coli and streptococcus aureus.
The reductive action of yellow crucian carp albumen antibacterial peptide liquid is utilized to be silver atoms (reaction conditions is gentle) by silver nitrate reduction, then be distributed in gelatin (abundance, and security is high) in liquid, the stretchiness and toughness that improve most telolemma and yellow crucian carp albumen antibacterial peptide liquid and gelatin interact.
Claims (6)
1. a preparation method for yellow crucian carp protein nano silver antibacterial film, it is characterized in that, described preparation method comprises the steps:
A) A liquid is prepared: in yellow crucian carp albumen antibacterial peptide liquid, add gelatin, be mixed with gelatin solution, in gelatin solution, add glycerine, abundant dissolving and mixing, obtain A liquid;
B) B liquid is prepared: configuration concentration is the silver nitrate solution of 500 μ g/mL;
C) masking: get the A liquid that step a) obtains, stir at 35-45 DEG C of lower magnetic force and be incubated 10-20min, then the B liquid that step b) obtains is added, mixed solution is temperature constant magnetic stirring 2.5-3.2h at 35-45 DEG C, mixed solution is adopted coating method film, dry 24-26h, obtains yellow crucian carp proteins nanometer silver antimicrobial film.
2. the preparation method of a kind of yellow crucian carp protein nano silver antibacterial film according to claim 1, it is characterized in that, in step a), the concentration of gelatin solution is 20-25%.
3. the preparation method of a kind of yellow crucian carp protein nano silver antibacterial film according to claim 1, it is characterized in that, the add-on of glycerine is the 25%-27% of gelatin weight.
4. the preparation method of a kind of yellow crucian carp protein nano silver antibacterial film according to claim 1, it is characterized in that, in step c), the add-on of B liquid is identical with the volume of A liquid.
5. the preparation method of a kind of yellow crucian carp protein nano silver antibacterial film according to claim 1 and 2, it is characterized in that, the feed postition of B liquid is for dropwise to add.
6. the preparation method of a kind of yellow crucian carp protein nano silver antibacterial film according to claim 1, it is characterized in that, the thickness of film is 1-2mm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105267951A (en) * | 2015-11-10 | 2016-01-27 | 韩盈 | Injury nursing composition containing nano-silver antibacterial ionized water |
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| WO2010031684A2 (en) * | 2008-09-17 | 2010-03-25 | Taminco | Antimicrobial composition |
| CN102816440A (en) * | 2012-09-10 | 2012-12-12 | 复旦大学 | Isolated soy protein-nano silver antibacterial composite membrane and preparation method thereof |
| CN102935521A (en) * | 2012-12-07 | 2013-02-20 | 苏州大学 | Method for preparing fibroin nano-silver hydrosol |
| CN103642070A (en) * | 2013-11-11 | 2014-03-19 | 青岛文创科技有限公司 | Antibiotic composite membrane of nano-silk fibroin-chitosan-nano-silver |
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- 2015-03-12 CN CN201510107297.6A patent/CN104817706A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010031684A2 (en) * | 2008-09-17 | 2010-03-25 | Taminco | Antimicrobial composition |
| CN102816440A (en) * | 2012-09-10 | 2012-12-12 | 复旦大学 | Isolated soy protein-nano silver antibacterial composite membrane and preparation method thereof |
| CN102935521A (en) * | 2012-12-07 | 2013-02-20 | 苏州大学 | Method for preparing fibroin nano-silver hydrosol |
| CN103642070A (en) * | 2013-11-11 | 2014-03-19 | 青岛文创科技有限公司 | Antibiotic composite membrane of nano-silk fibroin-chitosan-nano-silver |
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| 宋茹，汪东风，谢超，王铣: "黄鲫胃蛋白酶酶解液体外抗氧化、抑菌作用研究", 《食品科学》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105267951A (en) * | 2015-11-10 | 2016-01-27 | 韩盈 | Injury nursing composition containing nano-silver antibacterial ionized water |
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