CN104815095A - Composition with vaginal microecological balance repairing and recovering functions - Google Patents
Composition with vaginal microecological balance repairing and recovering functions Download PDFInfo
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Abstract
The invention relates to a composition with vaginal microecological balance repairing and recovering functions and a preparation method and application of the composition. The composition with the normal vaginal microecological environment repairing and recovering functions comprises, by weight, 1-10 parts of ecological nutrients, 1-8 parts of artificial cytomembranes, 0.2-6 parts of nano silver, 0.1-6 parts of tea polyphenol, 1-10 parts of aloe vera stoste, 0.1-5 parts of natural lactic acid and 1-8 parts of malt oligosaccharyl glucoside. By being verified by experiments, the composition with the normal vaginal microecological environment repairing and recovering functions is obtained by screening on the basis of a microecological theory studying on relations between microorganisms and a host, and by means of scientific compatibility and synergistic effects of the raw materials, and is prepared into a cosmeceutical or a medicine for repairing and recovering the normal vaginal microecological environment.
Description
Technical field
The invention belongs to sterile products and antibacterial product scope, relate to a kind of composition and method of making the same and the purposes that there is reparation and recover microecology in vaginas balance.
Background technology
Female genital tract is one of large microecosystem of female body four, the vagina of healthy women is parasitic more than one hundred million antibacterials, and Tiny ecosystem scholar is divided into resident bacterium from the rule that they haunt, pass by one's way bacterium and accidental bacterium, and few members has potential pathogenic effects.There is 85% lactobacillus had comparative advantage, be described as " vagina bodyguard ".Research shows: lactobacillus becomes lactic acid by the glycogen of estrogen decomposition vaginal epithelial cell, intravaginal pH value is maintained between 3.8 to 4.4 pin down under weakly acidic condition, restrict, coordinate the growth of all kinds of conditioned pathogen (harmful bacteria), therefore normal vagina liquid there are self-cleaning or bacteriostasis.As pH value in women's body unbalance (pH > 6), the hotbed of pathogenic bacterium will be become, occur a large amount of miscellaneous bacteria and leukocyte, bacillary, mycotic, trichomonal vaginitis may be brought out.
Any key element affecting ecosystem all can cause the phenomenon of some destruction of balance, and causes biological chain break.Balance between probiotic bacteria and pathogen is mainly based on the stability on micro organism quantity and kind.But the boundary line between probiotic bacteria and pathogen is difficult to determine usually: be considered to the strain of pathogen when causing any pathological changes when vaginal mucosal surfaces exists on a small quantity.When there are imbalances, the breeding increased suddenly will cause the disorder of flora.
Anatomical position residing for vagina is self-cleaning very unfavorable to vagina, and is open tract, logical cervix uteri in it, and outer even perineum, adjoins mutually with urethra and anus.The particularity of physiological environment, the feature of vagina and vulva warm moist, is applicable to the growth and breeding of multiple causal organism very much.Especially the rich protein-contg secretions in external genital position, very easily become the hotbed of causal organism breeding, therefore the sickness rate of women's urinary tract infection is more much higher than male.The investigation display to China women that World Health Organization (WHO) announces: have 75% women to infect a vaginitis to I haven't seen you for ages in life, wherein 2 to 3 patients become may cause infertile.The Chinese women Survey on Reproductive Health Status presided over by CDC mother and child care center shows, and once suffers from colpitic adult female and accounts for 53.86%, and wherein had people's recurrent exerbation of 9.48% to reach more than 4 times.
For a long time, the diagnosis and treatment theory of existing vaginal infection disease, all with the Therapeutic Method that killing microorganisms is main.A large amount of antibiotic, oral or remedy for external use (vaginal suppository, effervescent tablet etc.), and excessively clean lavation, while killing pathogenic bacterium, had a strong impact on intravaginal soda acid and colony balance, self-cleaning self-defence function is destroyed.General status is: single anti-inflammation and sterilization, does not consider microecological balance; Clinical sign and transference cure, pathogen disappear, and do not consider whether Tiny ecosystem restores balance.
Whole nation gynecological infections cooperative groups proposes: balance, microecology in vaginas as the ultimate aim of vaginitis treatment.Therefore, be necessary that the angle balanced from reparation and recovery microecology in vaginas is to develop new vaginal disinfection product and antibacterial product.
Summary of the invention
The object of this invention is to provide a kind of compositions that there is reparation and recover microecology in vaginas balance.
A kind of compositions having reparation and recover microecology in vaginas home of the present invention, comprises the raw material of following weight portion: ecoalimental element 1-10 part, artificial cell film 1-8 part, nanometer silver 0.2-6 part, tea polyphenols 0.1-6 part, aloe stoste 1-10 part, natural lactic acid 0.1-5 part, Fructus Hordei Germinatus oligose base glucosides 1-8 part.
According to the further feature of compositions of the present invention, described compositions comprises the raw material of following weight portion: ecoalimental element 2-6 part, artificial cell film 2-5 part, nanometer silver 0.5-4 part, tea polyphenols 0.3-3 part, aloe stoste 2-6 part, natural lactic acid 0.3-3 part, Fructus Hordei Germinatus oligose base glucosides 2-6 part.
Preferably, described compositions makes following external (containing in tract) dosage form: gel, spray, suppository.
Present invention also offers the preparation method of described compositions, comprise the following steps: ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, Fructus Hordei Germinatus oligose base glucosides are weighed respectively, gradation is added to the water, stir, add or do not add adjuvant routinely technique make acceptable preparation on pharmaceutics.
Preferably, can by thickening agent and polyhydric alcohol and water-soluble swollen dispersion, add ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, Fructus Hordei Germinatus oligose base glucosides, stir, technique is cold routinely joins, and adds or does not add adjuvant and make acceptable preparation on pharmaceutics.
Alternately, water first can be boiled and then be cooled to 80 DEG C, add adjuvant stirring and dissolving completely, homogenizing, temperature is down to the condition that technique allows, ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, Fructus Hordei Germinatus oligose base glucosides is added when guaranteeing bubble-free, stir, process heat is joined routinely, adds or does not add adjuvant and make acceptable preparation on pharmaceutics.
According to the further feature of the preparation method of compositions of the present invention, on described pharmaceutics, acceptable preparation is selected from following external (containing in tract) dosage form: gel, spray, suppository.
Present invention also offers the purposes of described compositions.
The medicine adornment for the preparation of repairing and recover microecology in vaginas home is used according to compositions of the present invention.
The medicine for the preparation of repairing and recover microecology in vaginas home is used according to compositions of the present invention.
Compositions of the present invention is on the basis of the microecology theory studying normal microflora and its host's mutual relation; the compositions with the function of reparation and recovery microecology in vaginas home filtered out; pass through scientific compatibility; play its mutual synergism; confirm a kind of compositions that there is reparation and recover microecology in vaginas home through experiment, external sterilizing or antibacterial product or medicine can be prepared further.
The present invention is with the food supplement of beneficial flora for Main way, based on faintly acid aqueous gel-type vehicle, is that various flora controls as auxiliary compound preparation with 5nm nanometer silver.Ecoalimental element can make probiotic bacteria recover quantity, and growth also will be faster than harmful bacteria, and pernicious bacteria can not as food.Even if natural balance is destroyed, the ecoalimental element in the present invention also can help probiotic bacteria to recover.
The present invention has surmounted the diagnosis and treatment theory of existing vaginal infection disease; by being that main Therapeutic Method becomes suppression, repairs and recover the novel therapeutic pattern of microecology in vaginas home with killing microorganisms, promote by the transformation of biomedical sterilization epoch to the antibacterial epoch of ecological medical science.Therefore, the present invention effectively solves traditional gynaecologic washing lotion while killing pathogenic bacterium, has killed probiotic bacteria, causes intravaginal dysbacteriosis, immunity low, causes the problem of vaginitis recurrent exerbation.
Compositions of the present invention can maintain vaginal mucosa physiological environment, while increase probiotic bacteria, reduce the quantity of pathogenic bacterium, maintenance microecology in vaginas balances, and improves vagina cleanness degree, improves vaginal mucosa immunity, and good biocompatibility, nonirritant, safety has no side effect, safer compared with other vaginal products.
Detailed description of the invention
In compositions of the present invention, the mechanism of action of each raw material components is as follows:
Ecoalimental element: preferably adopt French AXIALYS company to develop the product provided.It is the natural grape oligosaccharide be separated by patent enzyme process, the mixture formed with natural inulin extract.It comprises potent female reproductive system ecoalimental element raw material, can maintain and help to rebuild mucosa beneficial flora.It also comprises the carbon source of most of probiotic bacteria, and selective protection and being utilized by probiotic bacteria, its absorptance pathogen is faster and better, its special money voltinism, can not promote harmful bacteria.
Ecoalimental element has following effect: (1) breeds more probiotic bacteria, has enzyme, the protective substance of the even antibacterial activity of sterilizing by secretion, strengthens vaginal mucosal barrier, participates in cellular metabolism and immune system effect; (2) breed more probiotic bacteria, by competition nutrition, suppress harmful microbe breeding, for vaginal mucosa sets up the protective barrier of the harmful pathogenic microorganism of antagonism together, thus the breeding suppressing pathogen and the injury avoiding it to bring.
Artificial cell film: " Lipidure " product preferably adopting NOF Corp's exploitation.It is the bionical raw material of membrane structure of synthetic, by phospholipid and MPC(Phosphorylcholine base) produce separately the polymer be polymerized, this structure has splendid biocompatibility and controls the tack of protein and cell, is the requisite important material of artificial cell, artificial organ, artificial blood vessel and contact lens.It comprises the new type of safe material with shielding and moisture retention two kinds of functions, imitate the artificial cell film that human body cell film designs, this is the film that can breathe, acting on mucosa can the effectively absorption of Profilin matter and the bonding of cell, strengthen permeability and the homergy of mucosa, be equivalent to the barrier giving the many one decks of vaginal mucosa; Unique resistance to flushing (washing 10 times) rests on adeciduate feature on mucous layer; Than hyaluronic acid, there is better moisture absorbability and moisture retentivity; Both had the filming function of the hydrated gel film of phospholipid polymer, also have the seizure effect to excitor substance produced by hydrophobicity base from butyl methyl acrylate, both complement each other thus have given play to more preferably nursing role.Its chemical property is inactive, can not produce chemical reaction with other raw materials, even if long-term preservation also can not variable color, rotten.
Nanometer silver: preferable particle size is the nanometer silver product of 5 nm, is bought by commercial sources.Nanometer silver belongs to non-antibiotic antibacterial, can kill various pathogenic microorganism, and to pathogenic bacteria of drug-resistant as antibiotic-resistance E. coli, drug resistance golden staphylococci, drug-resistant pseudomonas aeruginosa, streptococcus pyogenes, drug resistance enterococcus, anaerobe etc. have comprehensive antibacterial activity.After nano-Ag particles is combined with the cell wall/film of pathogen, directly can enter thalline, sulfydryl (-SH) that is rapid and oxygen metabolism enzyme combines, make enzyme deactivation, block respiratory metabolism and make it be choked to death, effectively avoid causing recurrent exerbation obstinate because of drug resistance.
Tea polyphenols: can adopt existing commercially produced product, operative norm is QB2154-1995 " food additive tea polyphenols ".
Tea polyphenols is the All Pure Nature antioxidant food extracted from Folium Camelliae sinensis, has oxidation resistance strong, has no side effect, the features such as free from extraneous odour.Research shows, the catechins ECG in tea polyphenols and EGC and oxidation product theaflavin etc. thereof, have definite anti-radical action.
Aloe stoste: existing commercially produced product can be adopted, the aloe stoste (40 times concentrate, desolventing technology) of such as U.S. TERRY company.
Aloe stoste not only has the effect of antiphlogistic antibacterial, and can also impel the metabolism of cell regeneration and promotion cell, accelerates wound granulation promoting and heals very soon.Aloe stoste has following effect: the effect 1, strengthening immunologic function: the materials such as mucopolysaccharide (Polysaccharioes), mucin (Mucin) have facilitation to body Function of lymphocyte transform with to the vegetation of macrophage, can significantly improve the immunologic function of body; And mucin can make weak and sickly acidic physique change strong alkalescence body constitution into; 2, bactericidal action: Aloe pungent (Aloecin), Aloe saponin (Aloesaponin), Aloe resin B (Aloesin) etc. all have killing action to Klebsiella pneumonia, bacillus pyocyaneus, staphylococcus aureus, beta hemolysis streptococcus, streptococcus faecalis etc.; 3, by the co-operating antiinflammatory action of various effective ingredient: slow base peptidase, polysaccharide, Aloe resin B (Aloesin), Aloe (AloeutinA.B), the slow base peptidase Anhydroalkannin disease of aloin (A Laoyin Aloin), especially the Aloe gram tincture in Aloe polysaccharide and Aloe resin B, can cure scytitis; 4, the effect of wound healing is promoted: Aloe gram tincture (AloeutinA.B), wound hormone (1-decene-1 Traumaticid), polysaccharide peptide manna etc. have the effect of antiinflammatory, heat absorption detumescence, softening skin, enhancing cell viability; Can the healing of accelerated in wounds.
Natural lactic acid: existing commercially produced product can be adopted, operative norm: GB2023-2003.It is produced in lactobacillus effect bottom fermentation method by corn starch sugar, because the Pfansteihl part that is the intrinsic nature moisturizing factor of skin is widely used as the moisturizing agent of many skin care item.It is the gentleest hydroxy acid AHA, is also the main component maintaining vagina weak acid environment, can be used as pH value regulator.
Fructus Hordei Germinatus oligose base glucosides: existing commercially produced product can be adopted, the product that such as Japanese woods protobiochemistry institute provides.It is a kind of carbohydrate syrup be made up of starch, is made up of various saccharide, Cell protection, moisturizing, good maintenance mucosa softness, and the sliding sense of relaxing that expands; Have and alleviate because a variety of causes stimulates the inflammation and pruritus caused; Give the function of the TNF-a in cytoactive effect, the protective effect avoiding cell drying, inflammatory response; In the cultivation of anaerobe measures, show extremely strong low money voltinism tendency.
Compositions of the present invention comprises above-mentioned raw materials, can adopt and with the following method these raw materials is prepared into preparation:
(1) adopt with the composition being equivalent to described weight proportion for raw material adds thickening agent and stabilizing agent, according to process sequence mixing, stirring, homogenizing, obtain the gel meeting formulation requirements;
(2) adopt to be equivalent to the composition of described weight proportion relation for raw material, reduce or do not add thickening agent and stabilizing agent, after suitably processing, add solvent, be made into the liquid sprays meeting formulation requirements as required;
(3) adopt to be equivalent to the composition of described weight proportion relation for raw material, melting substrate (adjuvant), add composition (mixing), injection molding, cooling, scraping, taking-up, the suppository of formulation requirements must be met.
embodiment one:
Raw material: ecoalimental element 1g, artificial cell film 2g, nanometer silver 0.2g, tea polyphenols 0.1g, aloe stoste 1g, natural lactic acid 0.6g, Fructus Hordei Germinatus oligose base glucosides 2g
Take ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, the Fructus Hordei Germinatus oligose base glucosides of described weight proportion, appropriate and the pure water of polyacrylamide copolymer, xanthan gum, carbomer, glycerol, butanediol (boils, near room temperature) mixing dissolving, described composition is added respectively, stir, obtain gel.
embodiment two:
Raw material: ecoalimental element 4g, artificial cell film 8g, nanometer silver 1g, tea polyphenols 2g, aloe stoste 2g, natural lactic acid 1g, Fructus Hordei Germinatus oligose base glucosides 1g
Take ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, the Fructus Hordei Germinatus oligose base glucosides of described weight proportion, appropriate polyacrylamide copolymer, xanthan gum, carbomer, glycerol, butanediol are joined pure water and (boil, near room temperature), mixing is dissolved, homogenizing, gel, after still, described composition is added respectively, stirs, obtain gel.
embodiment three:
Raw material: ecoalimental element 5g, artificial cell film 3g, nanometer silver 6g, tea polyphenols 3g, aloe stoste 3g, natural lactic acid 5g, Fructus Hordei Germinatus oligose base glucosides 5g
Take ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, the Fructus Hordei Germinatus oligose base glucosides of described weight proportion, for subsequent use; Water used is first boiled and is then cooled to 80 DEG C, add adjuvant hydroxyethyl-cellulose, xanthan gum, carbomer, glycerol, butanediol stirring and dissolving completely, homogenizing, during near 40 DEG C of temperature, guarantee that bubble-free adds ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, Fructus Hordei Germinatus oligose base glucosides, stir, obtain gel.
embodiment four:
Raw material: ecoalimental element 3g, artificial cell film 1g, nanometer silver 3g, tea polyphenols 6g, aloe stoste 10g, natural lactic acid 0.1g, Fructus Hordei Germinatus oligose base glucosides 3g
Take ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, the Fructus Hordei Germinatus oligose base glucosides of described weight proportion, join pure water (boiling, near less than 45 DEG C), mixing is dissolved, 300 orders filter, and obtain liquid sprays.
embodiment five:
Raw material: ecoalimental element 10g, artificial cell film 5g, nanometer silver 4g, tea polyphenols 2g, aloe stoste 5g, natural lactic acid 2g, Fructus Hordei Germinatus oligose base glucosides 8g
First bolt mould is cleaned, dried, with lubricator coat model inside a little; The water soluble ingredient such as ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, Fructus Hordei Germinatus oligose base glucosides taking described weight proportion directly mixes with the water-soluble base melted, to slightly overflowing die orifice in impouring bolt mould, let cool, after solidifying completely, spilling part is cut with cutter, open model, suppository is released, obtains suppository.
More than citing is described in further detail foregoing of the present invention, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to above citing, and all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Obtained present component can to add on pharmaceutics acceptable adjuvant routinely technique be prepared into required preparation.As conventional polyacrylamide copolymer, xanthan gum, alginate jelly, carrageenan, Kappa mother, hydroxyethyl-cellulose, Radix Acaciae senegalis, cocoa butter, semi-synthetic cocos nucifera oil ester, the grey grease in semi-synthetic mountain, half platform become Petiolus Trachycarpi grease, propylene glycol stearate, glycerin gelatine, Polyethylene Glycol, glycerol, butanediol, propylene glycol etc.
Make the exterior-applied formulation of spray form, also can make the mucosa drug formulation of gel, suppository formulations form.
Adjuvant described here, can be different according to different preparations, as the diluent, thickening agent, antiseptic, stabilizing agent, surfactant, filler etc. commonly used at gel, suppository, spray etc.
Its conventional adjuvant is as microcrystalline Cellulose, Polyethylene Glycol, sorbitol, polyvidon, hydroxypropyl methylcellulose, methylcellulose, ethyl cellulose, carboxymethylstach sodium, hydroxypropyl cellulose, carrageenan, agar, gelatin, sodium alginate, xanthan gum, guar gum, tragcanth, arabic gum, locust bean gum, POLY-karaya, glyceryl monostearate, polyacrylamide, cross linked sodium polyacrylate, polyvinyl alcohol, carbomer, sorbic acid, potassium sorbate, ethyl hydroxybenzoate, nipalgin, glycerol, propylene glycol, BHT, BHA etc.
The beneficial effect of compositions of the present invention is set forth further below by way of experiment.The gel (hereinafter referred to as " this sample ") prepared by embodiment three is selected to carry out following experiment.
Embodiment six: flora is to the consumption quantifier elimination of the gel that compositions of the present invention is made
Experiment purpose: by the flora be separated from vagina, studies the degree that ecological nutrient is consumed.
Experimental technique: the flora of a part is transplanted in the control media containing 0.5% glucose.Glucose is thing in contrast, almost can be absorbed by all strains.
Another part is transplanted in the medium containing 0.5% glucose+5% gel sample.
The making of research sample: the sample (avoiding nanometer silver to resist microbicidal interference) not adding nanometer silver.
The medium of each flora is all identical.
After 48 hours, calculate the amount of residual glucose/ecoalimental element.
Criterion sees the following form 1.
Show the criterion of glucose or this sample be consumed in 1:48 hour
| Consumption level | Describe |
| 0 to 20% | No consumption |
| 20 to 40% | Consumption degree is poor |
| 40 to 60% | Medium consumption degree |
| 60 to 100% | High to all consuming |
Experimental result sees the following form 2 and table 3.
Table 2:
| Probiotic bacteria | Sample | Glucose control product |
| Kirschner storehouse micrococcus luteus | 60 to 100% | 60 to 100% |
| Addicted to oxygen gram-positive cocci | 20 to 40% | 0 to 20% |
| Head staphylococcus | 20 to 40% | 60 to 100% |
| Corynebacterium xerosis | 40 to 60% | 60 to 100% |
| Lactobacillus | 60 to 100% | 60 to 100% |
Table 3:
| Pathogen | Sample | Glucose control product |
| Staphylococcus | 0 to 20% | 60 to 100% |
| Corynebacterium | 0 to 20% | 60 to 100% |
| Propionibacterium acnes | 0 to 20% | 60 to 100% |
| Gartner antibacterial | 0 to 20% | 20 to 40% |
Conclusion:
1, sample is obviously the carbon source of most of tested probiotic bacteria, can be absorbed by beneficial flora rapidly.
2, contrary, sample can not obviously by the pathogenic microorganism metabolism in skin (vagina separation) ecosystem.Because it is not the carbon source of these microorganisms, but exists as biostatic agent.
3, sample be biological selectivity protect and utilized by probiotic bacteria.Probiotic bacteria is faster and better to the absorptance harmful bacteria of ecoalimental element, Molds and yeasts.Prove that this sample is potent ecoalimental element thus.
4, pudendum does not have cuticular protection, and its protection comes from prebiotic lactobacillus.Without physical protection, this balance is very easily because excessive cleaning and be destroyed.The probiotic bacteria biological selectivity that the display of this sample is higher.
Embodiment seven: competitive increment study
The experiment of embodiment six shows, compared with harmful bacteria, this sample can be absorbed by probiotic bacteria quickly.Thus make probiotic bacteria reproduction speed faster than harmful bacteria.From experiment, we find that the existence of this sample can promote the growth of probiotic bacteria really.
Experiment purpose: this test shows the impact of this sample when probiotic bacteria and pathogen or harmful bacteria exist simultaneously on flora growth.
Experimental technique: antibacterial is cultivated in containing the medium (tryptone USP) of 5% sample, calculates number of bacteria after 24 hours:
A) blending agent 1---competitive growth
Kirschner storehouse micrococcus luteus (probiotic bacteria)
Staphylococcus aureus (pathogen)
Test is carried out in same media, calculates bacterial number.
Experimental result sees the following form 4.
Table 4:
| The cultivation time | T=0 | T=6h | T=24h | T24h / T0 |
| Staphylococcus aureus (individual/ml) | 10 6 | 10 5 | 5x10 4 | Be less than 20 times |
| Kirschner storehouse micrococcus luteus (individual/ml) | 10 6 | 10 7 | 10 8 | Be greater than 100 times |
B) blending agent 2---competitive growth
Kirschner storehouse micrococcus luteus (probiotic bacteria)
Corynebacterium xerosis (fixing pernicious bacteria, forms abnormal flavour)
Test is carried out in same media, calculates bacterial number.
Experimental result sees the following form 5.
Table 5:
| The cultivation time | T=0 | T=6h | T=24h | T24h / T0 |
| Corynebacterium xerosis (individual/ml) | 10 6 | 5 X 10 5 | 10 5 | ~ be less than 10 times |
| Kirschner storehouse micrococcus luteus (individual/ml) | 10 6 | 10 7 | 10 8 | Be less than 100 times |
Conclusion:
Above result shows this sample can promote that probiotic bacteria (kirschner storehouse micrococcus luteus) grows, but harmful bacteria (Corynebacterium xerosis, staphylococcus aureus)) then can not be promoted, in default of food, quantity reduces gradually on the contrary.
Embodiment eight: the feasibility study that yeast is suppressed
Experiment purpose: determine whether this sample works to harmful saccharomycetic growth.
Experimental technique: adopt test concentrations to be the sample of 5%, selects candida albicans to test as harmful yeast strain.
Method of testing is as follows:
Under 35 ° of C, in the tryptone salt medium containing and do not contain sample, cultivate/dilute yeast 24 hours.
Yeast number: by the deposit of the 1mL in initial suspension, counts in husky Bao Ruo Shi agar.
All tests all carry out 2 times.
Experimental result sees the following form 6.
Table 6:
| The cultivation time | T=0 | T=24h | T24 h / T0 |
| Candida albicans | 10 7 | 10 6 | Be less than 10 times |
Conclusion:
1, this sample does not affect saccharomycetic growth.The breeding of candida albicans is aging sign.
2, the use of this sample to blood capillary and personal hygiene all without harm.It is by protection and strengthen beneficial flora thus affect blood capillary and vagina ecosystem.
Claims (10)
1. the compositions that there is reparation and recover microecology in vaginas home; it is characterized in that, described compositions comprises the raw material of following weight portion: ecoalimental element 1-10 part, artificial cell film 1-8 part, nanometer silver 0.2-6 part, tea polyphenols 0.1-6 part, aloe stoste 1-10 part, natural lactic acid 0.1-5 part, Fructus Hordei Germinatus oligose base glucosides 1-8 part.
2. compositions according to claim 1, it is characterized in that, described compositions comprises the raw material of following weight portion: ecoalimental element 2-6 part, artificial cell film 2-5 part, nanometer silver 0.5-4 part, tea polyphenols 0.3-3 part, aloe stoste 2-6 part, natural lactic acid 0.3-3 part, Fructus Hordei Germinatus oligose base glucosides 2-6 part.
3. compositions according to claim 1 and 2, is characterized in that, described compositions makes following external (containing in tract) dosage form: gel, spray, suppository.
4. the preparation method of compositions according to claim 1 and 2, it is characterized in that, comprise the following steps: ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, Fructus Hordei Germinatus oligose base glucosides are weighed respectively, gradation is added to the water, stir, add or do not add adjuvant routinely technique make acceptable preparation on pharmaceutics.
5. the preparation method of compositions according to claim 4, it is characterized in that: by thickening agent and polyhydric alcohol and water-soluble swollen dispersion, add ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, Fructus Hordei Germinatus oligose base glucosides, stir, technique is cold routinely joins, and adds or does not add adjuvant and make acceptable preparation on pharmaceutics.
6. the preparation method of compositions according to claim 4, it is characterized in that: water is first boiled and is then cooled to 80 DEG C, add adjuvant stirring and dissolving completely, homogenizing, temperature is down to the condition that technique allows, ecoalimental element, artificial cell film, nanometer silver, tea polyphenols, aloe stoste, natural lactic acid, Fructus Hordei Germinatus oligose base glucosides is added when guaranteeing bubble-free, stir, process heat is joined routinely, adds or does not add adjuvant and make acceptable preparation on pharmaceutics.
7. the preparation method of compositions according to claim 4, is characterized in that, on described pharmaceutics, acceptable preparation is selected from following external (containing in tract) dosage form: gel, spray, suppository.
8. the preparation method of the compositions according to claim 5 or 6, is characterized in that, on described pharmaceutics, acceptable preparation is selected from following external (containing in tract) dosage form: gel, spray, suppository.
9. compositions according to claim 1 and 2 is used for the application in the medicine adornment for the preparation of reparation and recovery microecology in vaginas home.
10. compositions according to claim 1 and 2 is used for the application in the medicine for the preparation of reparation and recovery microecology in vaginas home.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110151981A (en) * | 2019-05-31 | 2019-08-23 | 杨平芝 | A kind of composition and preparation method thereof repaired vagina and treat vaginitis |
| CN112675257A (en) * | 2020-12-24 | 2021-04-20 | 许仲田 | Gel for regulating female vaginal flora and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110151981A (en) * | 2019-05-31 | 2019-08-23 | 杨平芝 | A kind of composition and preparation method thereof repaired vagina and treat vaginitis |
| CN112675257A (en) * | 2020-12-24 | 2021-04-20 | 许仲田 | Gel for regulating female vaginal flora and preparation method and application thereof |
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