CN104813855A - Evaluation method of transgenic herbicide-resistant rice for environmental safety of cyrtohinus lividipennis - Google Patents
Evaluation method of transgenic herbicide-resistant rice for environmental safety of cyrtohinus lividipennis Download PDFInfo
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 192
- 235000009566 rice Nutrition 0.000 title claims abstract description 192
- 230000009261 transgenic effect Effects 0.000 title claims abstract description 77
- 230000002363 herbicidal effect Effects 0.000 title claims abstract description 36
- 239000004009 herbicide Substances 0.000 title claims abstract description 36
- 238000011156 evaluation Methods 0.000 title claims abstract description 21
- 230000007613 environmental effect Effects 0.000 title claims abstract description 10
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 209
- 230000000740 bleeding effect Effects 0.000 claims abstract description 75
- 241001556089 Nilaparvata lugens Species 0.000 claims abstract description 52
- 238000000034 method Methods 0.000 claims abstract description 29
- 235000013601 eggs Nutrition 0.000 claims abstract description 12
- 230000004083 survival effect Effects 0.000 claims abstract description 6
- 241000903269 Cyrtorhinus Species 0.000 claims description 66
- 108010000912 Egg Proteins Proteins 0.000 claims description 35
- 102000002322 Egg Proteins Human genes 0.000 claims description 35
- 230000000052 comparative effect Effects 0.000 claims description 35
- 210000004681 ovum Anatomy 0.000 claims description 35
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 22
- 240000001624 Espostoa lanata Species 0.000 claims description 22
- 239000012153 distilled water Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 241000607479 Yersinia pestis Species 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 9
- 229920000742 Cotton Polymers 0.000 claims description 6
- 230000004071 biological effect Effects 0.000 claims description 5
- 210000000481 breast Anatomy 0.000 claims description 4
- 239000003292 glue Substances 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 238000012360 testing method Methods 0.000 abstract description 10
- 241000238631 Hexapoda Species 0.000 abstract description 9
- 238000005259 measurement Methods 0.000 abstract description 2
- 239000000969 carrier Substances 0.000 abstract 2
- 238000011160 research Methods 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 6
- 239000010902 straw Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 244000062645 predators Species 0.000 description 4
- 241000193388 Bacillus thuringiensis Species 0.000 description 2
- 241000176086 Sogatella furcifera Species 0.000 description 2
- 230000003679 aging effect Effects 0.000 description 2
- 229940097012 bacillus thuringiensis Drugs 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 244000037671 genetically modified crops Species 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 241000678602 Creontiades pacificus Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 101000585157 Homo sapiens CST complex subunit STN1 Proteins 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 240000002582 Oryza sativa Indica Group Species 0.000 description 1
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 1
- 102100021683 Stonin-1 Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QLULGSLAHXLKSR-UHFFFAOYSA-N azane;phosphane Chemical compound N.P QLULGSLAHXLKSR-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000012812 general test Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 244000062804 prey Species 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Catching Or Destruction (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides an evaluation method of transgenic herbicide-resistant rice for environmental safety of cyrtohinus lividipennis. The evaluation method includes the steps: collecting rice plant bleeding sap of comparison samples; respectively absorbing the rice plant bleeding sap of comparison samples by carriers and then placing the carriers into a feeding container; placing the feeding container into a test box, placing adult cyrtohinus lividipennis into the test box and checking the survival number of the adult cyrtohinus lividipennis at regular time until the adult cyrtohinus lividipennis are died; placing rice stems with brown planthopper eggs into the feeding container, placing the adult cyrtohinus lividipennis into the test box and dissecting rice plants after 24h; analyzing the service lives of the adult cyrtohinus lividipennis and difference significance of brown planthopper egg predation quantities of the comparison samples. The method performs indoor measurement and is easy to control, high in accuracy and short in periodicity, the influence of the transgenic rice plant bleeding sap on the cyrtohinus lividipennis can be accurately and rapidly researched, and the novel method is provided for safety evaluation of the transgenic rice for natural enemy insects.
Description
Technical field
The invention belongs to genetically modified crops technical field of ecological risk assessment, be specifically related to Transgenic Resistant Herbicide paddy rice to predator Mirid, cyrtorhinus environmental safety evaluation method.
Background technology
At 2014 1 year more than 27.2 hundred million mu (China's arable area is 18.3 hundred million mu), its ecological environment security is more and more concerned the grown worldwide area of genetically modified crops.If Transgenic Resistant Herbicide paddy rice realizes commercial growth, will the important component part of field ecosystem be become, also will become an important component part in the integrated control of Scale in Paddy Field Rice Diseases crop smothering.The impact of transgenic paddy rice on non-target organism is an indispensable important content during transgenosis ecological environment security is evaluated.Non-target organism mainly comprises non-target phytophagy insect, pest natural enemy, neutrophilous organism and a few class of economic insects.
Rice fulgorid is one of temperate zone, the topmost insect in subtropics and Tropical Rice field, and Mirid, cyrtorhinus is the important predator of rice fulgorid ovum and nymph.Separately there are some researches show, when not having brown planthopper, Mirid, cyrtorhinus completes its history of life by directly taking food paddy rice juice (what Jingjing, 2013), and therefore, namely Mirid, cyrtorhinus can be used as predator and also can be used as plant eater in paddy ecosystem.
At present, transgenic paddy rice completes mainly through indirectly taking food paddy rice the safety evaluatio of Mirid, cyrtorhinus, such as Bernal(2002) etc. research show, with turn
bt(
bacillus thuringiensis, bacillus thuringiensis,Bt) and gene pest-resistant paddy rice is raised and the brown planthopper that obtains is fed Mirid, cyrtorhinus, growing compared with the control without significant difference of Mirid, cyrtorhinus.And about Mirid, cyrtorhinus directly take food transgenic paddy rice safety evaluatio research very few.
Bleeding sap is that plant is from the liquid dredging tissue spilling that is injured or that cut off.The main component of bleeding sap has moisture, mineral element, amino acid, acid amides, soluble sugar and plant hormone etc., and the quantity of plant bleeding sap and composition can be used as the index (Liang Jiansheng etc., 2003) of improving activity of root system.At present, the research about rice strain bleeding sap mainly concentrates on the mensuration of rice root vigor, there is not yet and reports the research of Mirid, cyrtorhinus safety evaluatio for Transgenic Resistant Herbicide paddy rice about rice strain bleeding sap.And during prior art collection bleeding sap, the general test tube that adopts tips upside down on Culm of Rice, connects bleeding sap with ParafilmTM test tube mouth, and easily miss when collecting bleeding sap like this, and also can spill when being taken out by test tube, collection efficiency is low.
Plant plays a part core in the relation of plant-phytophagy insect-predator, is also the important foundation finding the sustainable controlling soil moist of insect.Transgenic Resistant Herbicide paddy rice will play a significant role in preventing and killing weeds in rice field and hybrid rice seeds are produced, but Transgenic Resistant Herbicide paddy rice also changes rice pest and predator occurs and the potential of development may simultaneously.So, with Transgenic Resistant Herbicide rice strain bleeding sap for experiment material, research Transgenic Resistant Herbicide paddy rice is for the impact of Mirid, cyrtorhinus biological property and prey ability, both the ecological environment security that can be Transgenic Resistant Herbicide paddy rice provides new evaluation method, improve and develop existing Transgenic Resistant Herbicide paddy rice environmental safety assessment technique system, continuous enhancement we to the understanding of Transgenic Resistant Herbicide paddy rice environmental safety, also the population contributing to understanding paddy rice important pests brown planthopper and Natural Enemies of Insect Pests Mirid, cyrtorhinus thereof occurs and growth and decline, to the comprehensive regulation of Scale in Paddy Field Rice Diseases crop smothering, also there is important ecological significance.
Summary of the invention
The present invention is directed to above-mentioned present situation, provide a kind of Transgenic Resistant Herbicide paddy rice to the evaluation method of Mirid, cyrtorhinus environmental safety, after transgenosis rice strain bleeding sap is directly taken food on Mirid, cyrtorhinus, the impact of its Life of Adult and prey ability is studied, thus more comprehensively, the ecological security of the evaluation Herbicide-tolerant Transgenic Rice of science.
To this, the invention provides a kind of Transgenic Resistant Herbicide paddy rice to the evaluation method of Mirid, cyrtorhinus environmental safety, comprise the following steps:
Step S1: determine that the rice varieties of comparative sample obtains each comparative sample, collects the rice strain bleeding sap of described each comparative sample paddy rice respectively;
Step S2: the rice strain bleeding sap absorbing described each comparative sample paddy rice with carrier respectively, then the carrier absorbing rice strain bleeding sap is respectively charged in feeding service container the feeding service container obtaining each comparative sample, the sidewall of described feeding service container is provided with through hole; Prepare the chamber identical with sample size, the feeding service container of described each comparative sample is put into each chamber respectively;
Step S3: the Mirid, cyrtorhinus adult sprouted wings in 24 h is respectively charged into described step S2 and is equipped with in each chamber of corresponding comparative sample feeding service container, every 6 h check the survival number of Mirid, cyrtorhinus adult, until adult is dead;
Step S4: prepare each comparative sample paddy rice rice stem with brown planthopper ovum; Each comparative sample paddy rice rice stem with brown planthopper ovum being put into described step S2 is equipped with in each chamber of corresponding comparative sample feeding service container, the Mirid, cyrtorhinus female adult pest of sprouting wings in 24h is also encased in described each chamber, after 24h, dissect rice strain, obtain the quantity that brown planthopper ovum is preyed on by Mirid, cyrtorhinus;
Step S5: analyze the Life of Adult of Mirid, cyrtorhinus between described each comparative sample and the significance of difference of brown planthopper ovum predatory number, analyzes the impact on Mirid, cyrtorhinus biological property and prey ability of Transgenic Resistant Herbicide paddy rice.
Adopt technique scheme, carrier is loaded in feeding service container with holes, worm can be avoided directly to contact with carrier, decrease influence factor.If directly come to feed insect by carrier absorption rice strain bleeding sap, the area of insects contact liquid is large, easily bedews wing, health, affects experiment effect.Described carrier is have the material absorbing rice strain bleeding sap ability, preferred cotton, cotton balls or cotton.
As a further improvement on the present invention, described comparative sample rice varieties is three, is respectively Transgenic Resistant Herbicide paddy rice, non-transgenic parent control paddy rice and non-transgenic sense worm contrast paddy rice.
As a further improvement on the present invention, also comprise and replace rice strain bleeding sap feeding in contrast with distilled water, repeat the step of step S2 to step S5.Namely comprise: will the cotton balls of distilled water be soaked, load in another one feeding service container; Feeding service container containing the cotton balls soaking distilled water is put into another one chamber, and every 6h checks the survival number of Mirid, cyrtorhinus adult, until adult is dead; Be equipped with in the chamber of soaking distilled water carrier feeding service container described in the paddy rice rice stem of the arbitrary comparative sample with brown planthopper ovum is put into, the Mirid, cyrtorhinus female adult pest of sprouting wings in 24 h is put into and is also encased in chamber, be allowed to condition at predation brown planthopper ovum in chamber; After 24 h, dissect rice strain, obtain the quantity that brown planthopper ovum is preyed on by Mirid, cyrtorhinus, by the data data in contrast obtained.
As a further improvement on the present invention, in step S1, described rice strain bleeding sap adopts rice strain bleeding sap collection container to collect, and the top of described rice strain bleeding sap collection container is provided with rake; Collection method is: be first cut into by Culm of Rice skewed, and what the rake of described rice strain bleeding sap collection container and described Culm of Rice are cut into is skewedly connected, and collects rice strain bleeding sap.Adopt this technical scheme, what utilize the rake of rice strain bleeding sap collection container and described Culm of Rice to be cut into is skewedly connected, flow into after rice strain bleeding sap is drained into rake in rice strain bleeding sap collection container and collect again, this overcome the problem that when conventional method is collected, rice strain bleeding sap easily spills.
As a further improvement on the present invention, in step S2, described feeding service container is cylindrical, hollow pipe.
As a further improvement on the present invention, in described step S4, the method prepared with each comparative sample paddy rice rice stem of brown planthopper ovum is, 24h of being laid eggs on the rice stem of each comparative sample respectively by the female worm of bosom ovum brown planthopper, obtains the paddy rice rice stem of each comparative sample with brown planthopper ovum.
As a further improvement on the present invention, in described step S3, after the Mirid, cyrtorhinus adult sprouted wings in 24 h is encased in chamber, timing glue head dropper supplements the rice strain bleeding sap of described each comparative sample to carrier, ensures that carrier is moistening.
As a further improvement on the present invention, described Mirid, cyrtorhinus is the experimental population that indoor TN1 rice varieties is raised.
As a further improvement on the present invention, described brown planthopper for after raising a generation on TN1 paddy rice, the more different rice varieties accessed in worm cage or product fasten the population raised and obtain.
As a further improvement on the present invention, described carrier is for being at least one in cotton balls, cotton or cotton.
Compared with prior art, beneficial effect of the present invention is:
The invention provides the method for evaluating safety that a kind of Mirid, cyrtorhinus directly takes food transgenic paddy rice, the method can study transgenosis rice strain bleeding sap quickly and accurately on the impact of Mirid, cyrtorhinus; The method is at indoor measurement, and be easy to control, accuracy is high, periodically short, can provide new method for the safety evaluatio of transgenic paddy rice to natural enemy insect.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of an embodiment of the present invention rice strain bleeding sap collection mode;
Fig. 2 is the structural representation of the feeding device of an embodiment of the present invention;
Fig. 3 is that three kinds of rice strain bleeding saps of an embodiment of the present invention are to Mirid, cyrtorhinus female adult pest aging effects comparison diagram;
Fig. 4 is that three kinds of rice strain bleeding saps of an embodiment of the present invention are to Mirid, cyrtorhinus male imago aging effects comparison diagram;
Fig. 5 is that three kinds of rice strain bleeding saps of an embodiment of the present invention and distilled water affect comparison diagram to Mirid, cyrtorhinus predation brown planthopper ovum ability.
Wherein, CK: the experiment process of feeding distilled water;
The experiment process of STN1: feeding TN1 rice strain bleeding sap;
The experiment process of SD68: feeding D68 rice strain bleeding sap;
The experiment process of SBar68-1: feeding Bar68-1 rice strain bleeding sap.
In figure, a, b, ab represent otherness, and same letter represents that both differences are not remarkable, different letter representation significant difference.
Embodiment
The invention provides a kind of Transgenic Resistant Herbicide paddy rice to the evaluation method of Mirid, cyrtorhinus environmental safety, embodiment is, comprises the following steps:
Step S1: collect the rice strain bleeding sap that Transgenic Resistant Herbicide paddy rice, non-transgenic parent control paddy rice and non-transgenic sense worm contrast paddy rice respectively;
Step S2: absorb the rice strain bleeding sap that Transgenic Resistant Herbicide paddy rice, non-transgenic parent control paddy rice and non-transgenic sense worm contrast paddy rice respectively with cotton balls, then be respectively charged in three feeding service containers by the cotton balls absorbing rice strain bleeding sap, the sidewall of described feeding service container is provided with through hole; Three feeding service containers are respectively put in three chambers;
Step S3: the Mirid, cyrtorhinus adult sprouted wings in 24 h is respectively charged into in three chambers, every 6 h check the survival number of Mirid, cyrtorhinus adult, until adult is dead;
Step S4: 24h of being laid eggs on Transgenic Resistant Herbicide paddy rice Bar68-1, non-transgenic parent control paddy rice D68 and non-transgenic sense worm contrast paddy rice TN1 rice stem respectively by the female worm of bosom ovum brown planthopper, obtains the above-mentioned three kinds of paddy rice rice stems with brown planthopper ovum; These the three kinds paddy rice rice stems with brown planthopper ovum being respectively put into step S2 is equipped with in three chambers of the rice strain bleeding sap feeding service container of corresponding comparative sample, the Mirid, cyrtorhinus female adult pest of sprouting wings in 24h is respectively charged in these three chambers, after 24h, dissect rice strain, obtain the quantity that brown planthopper ovum is preyed on by Mirid, cyrtorhinus.
Step S5: between the rice bleeding sap feeding process of comparing the rice strain bleeding sap that adopts Transgenic Resistant Herbicide paddy rice and non-transgenic parent control, non-transgenic sense worm kind, the Life of Adult of Mirid, cyrtorhinus and the significance of difference of brown planthopper ovum predatory number, analyze the impact on Mirid, cyrtorhinus biological property and prey ability of Transgenic Resistant Herbicide paddy rice.
Below in conjunction with accompanying drawing, preferably embodiment of the present invention is described in further detail.
For examination worm source: brown planthopper and Mirid, cyrtorhinus are indoor TN1 rice varieties and raise population.Wherein, after brown planthopper raises a generation on TN1 paddy rice, then access on the different rice material paddy rice of worm cage and raise, obtain the Population of Rice Brown Planthopper of different rice material.
For examination rice varieties: Herbicide-tolerant Transgenic Rice " Bar68-1 " is the Institute of Subtropical Agriculture, The Chinese Academy of Sciences, the Indica rice of Hunan Research Centre for Hybrid Rice and Hong Kong Chinese University's incubation, be will with particle bombardment
baranti-careless ammonium phosphine transgenic paddy rice (the Xiao G. Y. that gene proceeds to rice variety " D68 " and obtains
et al., 2007), non-transgenic parent control kind is " D68 ", provides by the Institute of Subtropical Agriculture, The Chinese Academy of Sciences.Sense worm check variety " TN1 " is provided by Guangxi Academy of Agricultural Sciences's Plant Protection Institute.
Concrete steps are:
Step S1: collect the rice strain bleeding sap that Transgenic Resistant Herbicide paddy rice, non-transgenic parent control paddy rice and non-transgenic sense worm contrast paddy rice respectively;
Get the rice seedling of the Transgenic Resistant Herbicide paddy rice Bar68-1 of 60 ages in days, non-transgenic parent control paddy rice D68 and non-transgenic sense worm contrast paddy rice TN1, remove outer leaf sheath to retread and put, stem the first half is being cut off with the scissors through alcohol disinfecting from native 10 cm places after 24 h, as shown in Figure 1, Culm of Rice 1 is cut into skewed 2, the part that rice strain bleeding sap container 3 contacts with stem stalk also makes tilt port 4, is connected by the tilt port 4 of rice strain bleeding sap container 3 with the bottom of skewed 2.Adopt this technical scheme, collect again after utilizing rice strain bleeding sap to drain into skewed bottom, the problem that when conventional method is collected, rice strain bleeding sap easily spills can be overcome like this.
Step S2: be respectively put in Transgenic Resistant Herbicide paddy rice Bar68-1, the non-transgenic parent control paddy rice D68 of 60 ages in days and non-transgenic sense worm contrast paddy rice TN1 rice strain bleeding sap at cotton balls and soak, putting into cotton balls in addition again soaks in contrast at distilled water, not drip for standard; As shown in Figure 2, then by absorb Transgenic Resistant Herbicide paddy rice Bar68-1 rice strain bleeding sap, non-transgenic parent control paddy rice D68 rice strain bleeding sap, non-transgenic sense worm contrast paddy rice TN1 rice strain bleeding sap and distilled water cotton balls, be respectively charged in four feeding service container pipes 21, the sidewall of described feeding service container pipe 21 is provided with through hole 22; These four feeding service container pipes 21 are respectively put in four chambers 23.
Step S3: as shown in Figure 2, is respectively charged into the Mirid, cyrtorhinus adult sprouted wings in 10/case 24 h in four chambers, and covers test case lid 24, and described tryoff lid 24 is also provided with through hole 25; Every 6 h check the survival number of Mirid, cyrtorhinus adult, until adult is dead; Period need ensure that the moistening need of cotton balls supplement the rice strain bleeding sap of each comparative sample in time with glue head dropper.The each process of above-mentioned test (different rice material bleeding sap or distilled water contrast) repeats 3 times, i.e. each rice material test Mirid, cyrtorhinus female, each 30 of male imago, totally 60 adults, and 4 process shared Mirid, cyrtorhinus adults 240.
Step S4: the female worm of bosom ovum brown planthopper to be laid eggs respectively on Transgenic Resistant Herbicide paddy rice Bar68-1, non-transgenic parent control D68 and non-transgenic sense worm kind TN1 rice stem 24h with the quantity of 5/root, obtain the above-mentioned three kinds of paddy rice rice stems with brown planthopper ovum;
These the three kinds paddy rice rice stems with brown planthopper ovum are put into three chambers 23 of rice strain bleeding sap cotton balls feeding service container that corresponding Transgenic Resistant Herbicide paddy rice, non-transgenic parent control paddy rice and non-transgenic sense worm contrast paddy rice are housed described in step S2 respectively with the quantity of 1/case, then with the quantity of 1/case, the Mirid, cyrtorhinus female adult pest of sprouting wings in 24 h is respectively charged into in three chambers 23, is allowed to condition at the brown planthopper ovum on the different rice material of predation in case.After 24 h, dissect rice strain, obtain the quantity that brown planthopper ovum is preyed on by Mirid, cyrtorhinus.
Prepare the chamber 23 that two are equipped with distilled water cotton balls feeding service container in addition, together with the chamber 23 that distilled water cotton balls feeding service container is housed of step S3, be altogether three chambers 23 that distilled water cotton balls feeding service container is housed, above-mentioned three kinds of paddy rice rice stems with brown planthopper ovum are put into the quantity of 1/case the chamber 23 that three are equipped with distilled water cotton balls feeding service container respectively according to rice varieties, then with the quantity of 1/case, the Mirid, cyrtorhinus female adult pest of sprouting wings in 24 h is respectively charged in these three chambers 23, is allowed to condition at predation brown planthopper ovum in case; After 24 h, dissect rice strain, obtain the quantity that brown planthopper ovum is preyed on by Mirid, cyrtorhinus.
Above-mentioned test has six process altogether, that is:
Process A: produce and have the Bar68-1 rice straw of Bar68-1 Population of Rice Brown Planthopper pieces of an egg, for being soaked with the cotton balls of Bar68-1 bleeding sap (bleeding sap be used for feeding Mirid, cyrtorhinus adult with) in feeding service container;
Treatments B: produce the D68 rice straw having D68 Population of Rice Brown Planthopper pieces of an egg, for being soaked with the cotton balls of D68 bleeding sap in feeding service container;
Process C: produce the TN1 rice straw having TN1 Population of Rice Brown Planthopper pieces of an egg, for being soaked with the cotton balls of TN1 bleeding sap in feeding service container;
Process D: produce the Bar68-1 rice straw having Bar68-1 Population of Rice Brown Planthopper pieces of an egg, for being soaked with the cotton balls of distilled water in feeding service container;
Process E: produce the D68 rice straw having D68 Population of Rice Brown Planthopper pieces of an egg, for being soaked with the cotton balls of distilled water in feeding service container;
Process F: produce the TN1 rice straw having TN1 Population of Rice Brown Planthopper pieces of an egg, for being soaked with the cotton balls of distilled water in feeding service container.
Above-mentioned each process repetition 10 times, i.e. each process test Mirid, cyrtorhinus female adult pest 10, brown planthopper female adult pest 50,6 process share Mirid, cyrtorhinus female adult pest 60, brown planthopper female adult pest 300.
Step S5: compare Transgenic Resistant Herbicide paddy rice Bar68-1 bleeding sap, non-transgenic sense worm contrast paddy rice TN1 bleeding sap, between non-transgenic parent control paddy rice D68 bleeding sap and the process of distilled water blank, female, male imago life-span of Mirid, cyrtorhinus and the significance of difference of brown planthopper ovum predatory number, analyze the impact of Transgenic Resistant Herbicide paddy rice on Mirid, cyrtorhinus biological property and prey ability.
The result display of this example, as shown in Figure 3 and Figure 4, Mirid, cyrtorhinus is after taking food Transgenic Resistant Herbicide paddy rice Bar68-1 bleeding sap, and female, the male imago life-span is compared with the bleeding sap that non-transgenic sense worm contrasts TN1 with non-transgenic parent control D68, and difference is not remarkable.
As shown in Figure 5, can be found by the contrast between above-mentioned six process, no matter whether there is the existence of rice strain bleeding sap, significant difference is there is not in the prey ability of Mirid, cyrtorhinus between three kinds of rice materials, namely Transgenic Resistant Herbicide paddy rice Bar68-1 is on the impact of Mirid, cyrtorhinus adult predation brown planthopper ovum, and to contrast the impact that TN1 preys on brown planthopper ovum to Mirid, cyrtorhinus adult consistent with non-transgenic parent control D68 and non-transgenic sense worm.
Above content is in conjunction with concrete preferred embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (10)
1. Transgenic Resistant Herbicide paddy rice is to the evaluation method of Mirid, cyrtorhinus environmental safety, it is characterized in that, comprises the following steps:
Step S1: determine that the rice varieties of comparative sample obtains each comparative sample, collects the rice strain bleeding sap of described each comparative sample paddy rice respectively;
Step S2: the rice strain bleeding sap absorbing described each comparative sample paddy rice with carrier respectively, then the carrier absorbing rice strain bleeding sap is respectively charged in feeding service container the feeding service container obtaining each comparative sample, the sidewall of described feeding service container is provided with through hole; Prepare the chamber identical with sample size, the feeding service container of described each comparative sample is put into each chamber respectively;
Step S3: the Mirid, cyrtorhinus adult sprouted wings in 24 h is respectively charged into described step S2 and is equipped with in each chamber of corresponding comparative sample feeding service container, every 6 h check the survival number of Mirid, cyrtorhinus adult, until adult is dead;
Step S4: prepare the described each comparative sample paddy rice rice stem with brown planthopper ovum; Each comparative sample paddy rice rice stem with brown planthopper ovum being put into described step S2 is equipped with in each chamber of corresponding comparative sample feeding service container, the Mirid, cyrtorhinus female adult pest of sprouting wings in 24h is also encased in described each chamber, after 24h, dissect rice strain, obtain the quantity that brown planthopper ovum is preyed on by Mirid, cyrtorhinus;
Step S5: the significance of difference analyzing the Life of Adult of Mirid, cyrtorhinus between described each comparative sample and Mirid, cyrtorhinus predation brown planthopper ovum amount, analyzes the impact of Transgenic Resistant Herbicide paddy rice on Mirid, cyrtorhinus biological property and prey ability.
2. evaluation method according to claim 1, is characterized in that: the rice varieties of described each comparative sample is three, is respectively Transgenic Resistant Herbicide paddy rice, non-transgenic parent control paddy rice and non-transgenic sense worm contrast paddy rice.
3. evaluation method according to claim 1, is characterized in that: also comprise and replace rice strain bleeding sap feeding in contrast with distilled water, the step of repeating said steps S2 to step S5.
4. the evaluation method according to claim 1 ~ 3 any one, is characterized in that: in step S1, and described rice strain bleeding sap adopts rice strain bleeding sap collection container to collect, and the top of described rice strain bleeding sap collection container is provided with rake; Concrete operations comprise first is cut into skewed by Culm of Rice, what the rake of described rice strain bleeding sap collection container and described Culm of Rice are cut into is skewedly connected, and collects rice strain bleeding sap.
5. the evaluation method according to claim 1 ~ 3 any one, is characterized in that: in step S2, and described feeding service container is cylindrical, hollow pipe.
6. the evaluation method according to claim 1 ~ 3 any one, it is characterized in that: in described step S4, the method prepared with each comparative sample paddy rice rice stem of brown planthopper ovum is, bosom ovum brown planthopper female worm to be laid eggs respectively on the rice stem of each comparative sample 24h, obtain the paddy rice rice stem of each comparative sample with brown planthopper ovum.
7. the evaluation method according to claim 1 ~ 3 any one, it is characterized in that: in described step S3, after the Mirid, cyrtorhinus adult sprouted wings in 24 h is encased in chamber, timing glue head dropper supplements the rice strain bleeding sap of described each comparative sample to carrier, ensures that carrier is moistening.
8. the evaluation method according to claim 1 ~ 3 any one, is characterized in that: described Mirid, cyrtorhinus is the experimental population that indoor TN1 rice varieties is raised.
9. the evaluation method according to claim 1 ~ 3 any one, is characterized in that: described brown planthopper for after raise a generation on TN1 paddy rice, then is accessed on the different rice materials in worm cage and raises the population obtained.
10. the evaluation method according to claim 1 ~ 3 any one, is characterized in that: described carrier is at least one in cotton balls, cotton or cotton.
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